AlfalfaGEDB | Msa0591970

M. sativa cultivar zhongmu-4 / Msa0591970

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591970	XP_013455021.2	86.901	313	39	1	1	313	1	311	0.0	569

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591970	sp\|Q8L9Y3\|ARR14_ARATH	38.403	263	121	5	7	232	4	262	2.16e-53	182

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0591970	TF	GARP-ARR-B

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591970	MtrunA17_Chr4g0009851	84.076	314	47	2	1	313	1	312	0.0	544
Msa0591970	MtrunA17_Chr4g0009981	86.207	261	34	1	53	313	1	259	8.27e-169	469
Msa0591970	MtrunA17_Chr4g0009901	75.207	242	58	1	1	242	1	240	2.05e-128	367
Msa0591970	MtrunA17_Chr4g0011071	78.571	140	28	1	55	194	35	172	4.16e-74	225
Msa0591970	MtrunA17_Chr6g0471041	46.075	293	139	8	10	296	14	293	1.78e-69	221
Msa0591970	MtrunA17_Chr1g0161431	37.808	365	160	9	10	309	11	373	1.85e-67	222
Msa0591970	MtrunA17_Chr4g0009991	78.992	119	22	2	101	219	1	116	5.38e-58	182
Msa0591970	MtrunA17_Chr3g0123131	39.526	253	120	5	10	231	13	263	1.94e-55	189
Msa0591970	MtrunA17_Chr8g0376881	39.313	262	126	6	9	239	24	283	8.48e-55	186
Msa0591970	MtrunA17_Chr2g0294911	38.868	265	134	4	10	248	29	291	1.12e-53	184

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591970	AT2G01760.1	38.403	263	121	5	7	232	4	262	2.20e-54	182
Msa0591970	AT1G67710.1	39.700	267	125	8	11	243	8	272	6.85e-52	179
Msa0591970	AT3G16857.1	36.364	308	138	11	10	259	33	340	3.07e-49	174
Msa0591970	AT3G16857.2	36.364	308	138	11	10	259	33	340	4.29e-49	174
Msa0591970	AT2G01760.2	35.052	291	120	7	7	232	4	290	1.78e-48	167
Msa0591970	AT4G31920.1	35.622	233	140	2	10	232	13	245	3.62e-44	159
Msa0591970	AT2G25180.1	38.333	240	124	6	10	226	13	251	2.77e-42	154
Msa0591970	AT3G16857.3	34.572	269	118	9	49	259	2	270	5.16e-38	142
Msa0591970	AT5G58080.1	36.614	254	127	7	10	236	14	260	9.20e-38	142
Msa0591970	AT1G67710.2	39.713	209	91	7	68	243	1	207	2.18e-34	130

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Find 58 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAACACTTGCAAAGA+AGG	0.435703	4_2:-16962611	Msa0591970:CDS
AAAAGTGTTGCTCATAATGT+TGG	0.490442	4_2:+16961808	None:intergenic
AAAATGAACCTGTAAATGGC+TGG	0.527802	4_2:+16962366	None:intergenic
AAGTCATAAACATCCATGTC+TGG	0.491105	4_2:+16963008	None:intergenic
AATAAAGAATATGAAATTGT+TGG	0.416766	4_2:-16962688	Msa0591970:CDS
AATAAATTCTTACTAATGAC+AGG	0.461511	4_2:+16962954	None:intergenic
AATGTGGGGCTTGCGAATAT+TGG	0.385194	4_2:-16962786	Msa0591970:CDS
AATTGCAATAACCTTGAGAC+CGG	0.581075	4_2:+16963282	None:intergenic
ACATATTGCCAGCCATTTAC+AGG	0.282271	4_2:-16962374	Msa0591970:intron
ACATCTCCTCAATAGAGTTA+AGG	0.467190	4_2:+16963242	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAGTTACCAAATGTAGTA+TGG	-	chr4_2:16961965-16961984	Msa0591970:CDS	30.0%
CATGAAATCTATTGAATGTG+GGG	-	chr4_2:16962264-16962283	Msa0591970:intron	30.0%
TAAGCCTTTGATTGAAAAAG+AGG	-	chr4_2:16962303-16962322	Msa0591970:intron	30.0%
CATGTTGCAAGAATTAGAAA+CGG	-	chr4_2:16962343-16962362	Msa0591970:intron	30.0%
ATATGAAATTGTTGGAAGCT+CGG	-	chr4_2:16962384-16962403	Msa0591970:CDS	30.0%
GGTTCAAGAAAATAGAAAAC+GGG	-	chr4_2:16962408-16962427	Msa0591970:CDS	30.0%
GTTCAAGAAAATAGAAAACG+GGG	-	chr4_2:16962409-16962428	Msa0591970:CDS	30.0%
AAAAGAAACACTTGCAAAGA+AGG	-	chr4_2:16962453-16962472	Msa0591970:intron	30.0%
AACTGAGAAACTATAGACTT+AGG	+	chr4_2:16962766-16962785	None:intergenic	30.0%
TGATTTACAACAACAGACTT+AGG	+	chr4_2:16962797-16962816	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	16961748	16963338	16961748	ID=Msa0591970;Name=Msa0591970
chr4_2	mRNA	16961748	16963338	16961748	ID=Msa0591970-mRNA-1;Parent=Msa0591970;Name=Msa0591970-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0\|0\|1\|1\|1\|5\|0\|313
chr4_2	exon	16963221	16963338	16963221	ID=Msa0591970-mRNA-1:exon:4910;Parent=Msa0591970-mRNA-1
chr4_2	exon	16962967	16963116	16962967	ID=Msa0591970-mRNA-1:exon:4909;Parent=Msa0591970-mRNA-1
chr4_2	exon	16962542	16962853	16962542	ID=Msa0591970-mRNA-1:exon:4908;Parent=Msa0591970-mRNA-1
chr4_2	exon	16962375	16962451	16962375	ID=Msa0591970-mRNA-1:exon:4907;Parent=Msa0591970-mRNA-1
chr4_2	exon	16961748	16962032	16961748	ID=Msa0591970-mRNA-1:exon:4906;Parent=Msa0591970-mRNA-1
chr4_2	CDS	16963221	16963338	16963221	ID=Msa0591970-mRNA-1:cds;Parent=Msa0591970-mRNA-1
chr4_2	CDS	16962967	16963116	16962967	ID=Msa0591970-mRNA-1:cds;Parent=Msa0591970-mRNA-1
chr4_2	CDS	16962542	16962853	16962542	ID=Msa0591970-mRNA-1:cds;Parent=Msa0591970-mRNA-1
chr4_2	CDS	16962375	16962451	16962375	ID=Msa0591970-mRNA-1:cds;Parent=Msa0591970-mRNA-1
chr4_2	CDS	16961748	16962032	16961748	ID=Msa0591970-mRNA-1:cds;Parent=Msa0591970-mRNA-1

Gene Sequence

>Msa0591970

ATGGCAGTTTTCACTGACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTCTCCTTAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAAAAAGTTCTATCATGAAATCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGATTGAAAAAGAGGTCAAGATCATGTGGCAACATGTTGCAAGAATTAGAAACGGTTTGATTGAAAATAAAGAATATGAAATTGTTGGAAGCTCGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCACGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATGGGGCAACGCCAAAAACGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATATTGCCAGCCATTTACAGAAATATAGAAATCGGTTAAAGAGCTCAACAGAAGAGACAGAAGGTAGGATGAAAAAATCAAAGACGAGTCCTCTTGAAACAAAATTTGACTTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACGACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA

Protein sequence

>Msa0591970

MAVFTDNPSFPAGLKVIAIDHDITLLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTKSSIMKSIECGACEYWTKPLIEKEVKIMWQHVARIRNGLIENKEYEIVGSSVVQENRKRGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDGATPKTILKIMDVPDMKKEHIASHLQKYRNRLKSSTEETEGRMKKSKTSPLETKFDLEATHSMPEQDQSFLLNSATQCRDNCDAQRHWVEIGESNIMSNTFSDLPNLFPYVDDDLSSLVW*