AlfalfaGEDB | Msa0591990

M. sativa cultivar zhongmu-4 / Msa0591990

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591990	XP_013455021.2	70.498	261	55	1	1	239	1	261	1.40e-115	355
Msa0591990	XP_013455021.2	80.707	311	36	2	259	547	3	311	1.50e-176	510

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591990	sp\|Q8L9Y3\|ARR14_ARATH	35.115	262	107	7	263	465	4	261	4.48e-40	152
Msa0591990	sp\|Q8L9Y3\|ARR14_ARATH	42.667	150	82	3	7	154	4	151	6.32e-31	127

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591990	A0A396I0H3	64.504	262	70	2	1	239	1	262	1.64e-102	321
Msa0591990	A0A396I0H3	76.282	312	49	3	259	547	3	312	7.64e-165	479

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TFs/TRs:

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Gene ID	Type	Classification
Msa0591990	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591990	MtrunA17_Chr4g0009851	76.282	312	49	3	259	547	3	312	1.47e-168	479
Msa0591990	MtrunA17_Chr4g0009851	64.504	262	70	2	1	239	1	262	3.16e-106	321
Msa0591990	MtrunA17_Chr4g0009981	77.778	261	34	2	309	547	1	259	1.12e-141	409
Msa0591990	MtrunA17_Chr4g0009981	64.593	209	52	1	53	239	1	209	1.49e-79	250
Msa0591990	MtrunA17_Chr4g0009901	75.954	262	41	1	1	240	1	262	6.07e-136	395
Msa0591990	MtrunA17_Chr4g0009901	70.711	239	46	2	260	476	4	240	6.39e-107	321
Msa0591990	MtrunA17_Chr4g0011071	77.119	118	25	1	311	428	35	150	2.53e-56	186
Msa0591990	MtrunA17_Chr4g0011071	73.451	113	30	0	58	170	38	150	8.50e-54	180
Msa0591990	MtrunA17_Chr4g0010001	89.535	86	9	0	260	345	4	89	5.26e-51	169
Msa0591990	MtrunA17_Chr4g0010001	83.146	89	15	0	1	89	1	89	1.69e-48	162

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591990	AT2G01760.1	35.115	262	107	7	263	465	4	261	4.56e-41	152
Msa0591990	AT2G01760.1	42.667	150	82	3	7	154	4	151	6.43e-32	127
Msa0591990	AT1G67710.1	34.457	267	117	6	267	477	8	272	5.51e-39	149
Msa0591990	AT1G67710.1	34.836	244	108	4	11	203	8	251	4.74e-34	135
Msa0591990	AT2G01760.2	31.724	290	107	8	263	465	4	289	1.41e-35	138
Msa0591990	AT2G01760.2	35.955	178	82	4	7	154	4	179	1.98e-26	112
Msa0591990	AT4G31920.1	32.129	249	134	4	266	482	13	258	7.66e-34	135
Msa0591990	AT4G31920.1	33.476	233	121	5	10	208	13	245	7.99e-30	124
Msa0591990	AT2G25180.1	33.745	243	109	4	266	460	13	251	6.05e-32	130
Msa0591990	AT2G25180.1	32.787	244	110	4	10	203	13	252	3.35e-28	119

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Find 100 sgRNAs with CRISPR-Local

Find 463 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTAACCTGTAAATGGC+TGG	0.535893	4_2:+17030282	None:intergenic
AAAAGAAACACATGCAAAGA+AGG	0.478150	4_2:-17037339	Msa0591990:CDS
AAAAGAAACACTTGCAAAGA+AGG	0.435703	4_2:-17030529	Msa0591990:CDS
AAAGTGACTAACCTTGATAA+TGG	0.378712	4_2:+17037930	None:intergenic
AAGAAGACGTCAAGAGCATG+TGG	0.649026	4_2:-17037469	Msa0591990:CDS
AAGTCATAAACATCCATGTT+TGG	0.465091	4_2:+17030930	None:intergenic
AAGTCATAAGCATCCATGTT+TGG	0.471983	4_2:+17037705	None:intergenic
AATAAAGAATATGAAATTGT+TGG	0.416766	4_2:-17030606	Msa0591990:CDS
AATAAAGAATATGAAATTGT+TGG	0.416766	4_2:-17037416	Msa0591990:CDS
AATAAATTCTTACTAATGAC+AGG	0.461511	4_2:+17030876	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGTGACTAACCTTGATAA+TGG	+	chr4_2:17029748-17029767	None:intergenic	30.0%
CATTCTTTCCAACAAATTCA+AGG	+	chr4_2:17029914-17029933	None:intergenic	30.0%
TATGAAGTCTATTCAATGTG+GGG	-	chr4_2:17030153-17030172	Msa0591990:intron	30.0%
ATATGAAATTGTTGGAAGCT+TGG	-	chr4_2:17030267-17030286	Msa0591990:intron	30.0%
GGTTCAAGAAAATAGAAAAC+GGG	-	chr4_2:17030291-17030310	Msa0591990:CDS	30.0%
GTTCAAGAAAATAGAAAACG+GGG	-	chr4_2:17030292-17030311	Msa0591990:CDS	30.0%
AAAAGAAACACATGCAAAGA+AGG	-	chr4_2:17030336-17030355	Msa0591990:CDS	30.0%
AATTGCATCAACGATTTCTA+TGG	-	chr4_2:17030380-17030399	Msa0591990:intron	30.0%
ATTGCATCAACGATTTCTAT+GGG	-	chr4_2:17030381-17030400	Msa0591990:intron	30.0%
AGGAAACAATCATACTATCA+AGG	+	chr4_2:17030423-17030442	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	17029638	17038059	17029638	ID=Msa0591990;Name=Msa0591990
chr4_2	mRNA	17029638	17038059	17029638	ID=Msa0591990-mRNA-1;Parent=Msa0591990;Name=Msa0591990-mRNA-1;_AED=0.14;_eAED=0.15;_QI=0\|0\|0\|1\|0.77\|0.8\|10\|0\|547
chr4_2	exon	17037942	17038059	17037942	ID=Msa0591990-mRNA-1:exon:4948;Parent=Msa0591990-mRNA-1
chr4_2	exon	17037664	17037813	17037664	ID=Msa0591990-mRNA-1:exon:4947;Parent=Msa0591990-mRNA-1
chr4_2	exon	17037336	17037575	17037336	ID=Msa0591990-mRNA-1:exon:4946;Parent=Msa0591990-mRNA-1
chr4_2	exon	17037104	17037180	17037104	ID=Msa0591990-mRNA-1:exon:4945;Parent=Msa0591990-mRNA-1
chr4_2	exon	17036793	17036982	17036793	ID=Msa0591990-mRNA-1:exon:4944;Parent=Msa0591990-mRNA-1
chr4_2	exon	17031171	17031281	17031171	ID=Msa0591990-mRNA-1:exon:4943;Parent=Msa0591990-mRNA-1
chr4_2	exon	17030889	17031038	17030889	ID=Msa0591990-mRNA-1:exon:4942;Parent=Msa0591990-mRNA-1
chr4_2	exon	17030526	17030771	17030526	ID=Msa0591990-mRNA-1:exon:4941;Parent=Msa0591990-mRNA-1
chr4_2	exon	17030291	17030367	17030291	ID=Msa0591990-mRNA-1:exon:4940;Parent=Msa0591990-mRNA-1
chr4_2	exon	17029638	17029922	17029638	ID=Msa0591990-mRNA-1:exon:4939;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17037942	17038059	17037942	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17037664	17037813	17037664	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17037336	17037575	17037336	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17037104	17037180	17037104	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17036793	17036982	17036793	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17031171	17031281	17031171	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17030889	17031038	17030889	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17030526	17030771	17030526	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17030291	17030367	17030291	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1
chr4_2	CDS	17029638	17029922	17029638	ID=Msa0591990-mRNA-1:cds;Parent=Msa0591990-mRNA-1

Gene Sequence

>Msa0591990

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCAAGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAATGGACTGTTTCGATTTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTAAAACTCAACATTCCTGTCATTATGATGGCTGTTGATTTCGCAAAAAGTTCTATTATGAAGTCTATTCAATGTGGGGCTTGTACATATTGGACTAAGCCTTTGGTTGAAGAAGACGTCAAGAGCATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGATAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCAGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACGTGGGAAGAAAAAATCAAAGTGGAGTGATAGCTTTCAAGACGAGTGCATTGACAAGGTTCATAGTGATGGCTTTCAAACTCAATCGATCGATCAGGACCAGAACGATAACTTTCAAACTGAGCTGACTGATCAGGACCGGAATGATGTTTTCACTGACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTGTCCTCAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGTTACCAAATGCAATACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAAACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAGCAAGTTCTATCATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGACCATGTGGCAACATGTAGTAAGAATTAGAAACTGTTTGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTCGGTGGTTCAAAAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGAAGTATAGAAATTATTTAAAGAGCTCAACCGAAGAGAAAAAAGGTAGAATGAAAAAATCAAAGAGGAGTCCTTTTGAAACACAATTTAGCGTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCGACCACACAATGTCATGACAATTGCGACACACGACAACAATGGATGGAAATTAGTGAATCCGACATTACTAACAACATTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA

Protein sequence

>Msa0591990

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYQVTKCNTASDALNLLERMDCFDLMLIDAHMPNMDAYDFVQNVTLKLNIPVIMMAVDFAKSSIMKSIQCGACTYWTKPLVEEDVKSMWQHVVRNGSTENKEYEIVGSLVVQENRKRGREDDNASKETHAKKAQARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKRGKKKSKWSDSFQDECIDKVHSDGFQTQSIDQDQNDNFQTELTDQDRNDVFTDNPSFPAGLKVIAIDHDITVLNSIEEMCNRYHYQVTKCNTASDALNLLERKDCFDVMLIDAHMPNMDVYDFVQNVTLQLNIPVIMMAVDSTASSIMKSIECGACEYWTKPLVEKEVKTMWQHVVRIRNCLTENKEYEIVGSSVVQKNRKRGREDANASKETLAKKARATPKKILKIMDVPDMKKEHVASHLQKYRNYLKSSTEEKKGRMKKSKRSPFETQFSVEATHSMPEQDQSFLLNSTTQCHDNCDTRQQWMEISESDITNNIFSDLPNLFPYVDDDLSSLVW*