AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0593820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0593820 MtrunA17_Chr4g0012071 94.569 534 27 2 93 625 20 552 0.0 1029
Msa0593820 MtrunA17_Chr7g0230771 54.095 525 190 9 143 625 217 732 1.97e-169 500
Msa0593820 MtrunA17_Chr7g0230771 35.122 205 103 9 1 189 4 194 1.00e-12 71.2
Msa0593820 MtrunA17_Chr7g0230731 53.524 525 193 9 143 625 214 729 5.80e-166 491
Msa0593820 MtrunA17_Chr8g0373561 34.324 370 225 10 267 625 180 542 2.67e-53 191
Msa0593820 MtrunA17_Chr4g0038761 28.150 373 231 11 272 624 87 442 4.05e-32 129
Msa0593820 MtrunA17_Chr8g0376381 25.967 362 252 6 270 624 170 522 4.80e-32 130
Msa0593820 MtrunA17_Chr2g0306641 26.570 414 259 12 231 625 101 488 7.27e-32 129
Msa0593820 MtrunA17_Chr7g0224271 27.934 426 256 15 230 625 270 674 9.73e-32 130
Msa0593820 MtrunA17_Chr5g0445331 26.862 376 249 9 270 624 162 532 1.00e-31 129
Msa0593820 MtrunA17_Chr4g0039661 29.897 388 221 15 266 624 190 555 7.55e-31 127
Msa0593820 MtrunA17_Chr3g0110971 25.995 377 248 8 270 624 220 587 2.24e-30 126
Msa0593820 MtrunA17_Chr3g0101141 25.326 383 246 11 268 624 174 542 4.97e-30 124
Msa0593820 MtrunA17_Chr5g0447691 24.661 369 262 7 270 624 178 544 1.26e-29 123
Msa0593820 MtrunA17_Chr7g0245601 26.829 369 233 8 274 624 443 792 6.55e-28 119
Msa0593820 MtrunA17_Chr2g0290241 25.132 378 256 10 268 625 56 426 6.42e-27 114
Msa0593820 MtrunA17_Chr2g0319091 24.163 418 268 11 235 623 181 578 1.68e-26 114
Msa0593820 MtrunA17_Chr4g0057291 25.722 381 244 11 270 623 293 661 1.38e-25 112
Msa0593820 MtrunA17_Chr1g0159461 26.882 372 250 11 270 624 226 592 9.06e-25 108
Msa0593820 MtrunA17_Chr4g0076091 25.066 379 251 10 270 625 176 544 9.20e-25 108
Msa0593820 MtrunA17_Chr3g0125811 25.538 372 255 8 270 624 204 570 1.50e-24 108
Msa0593820 MtrunA17_Chr1g0183641 25.995 377 236 12 270 625 124 478 5.87e-24 105
Msa0593820 MtrunA17_Chr5g0424131 25.490 408 263 15 250 625 102 500 1.19e-21 99.0
Msa0593820 MtrunA17_Chr3g0114841 28.239 301 199 10 337 625 206 501 1.47e-21 98.6
Msa0593820 MtrunA17_Chr5g0403741 27.778 234 151 8 401 625 51 275 3.60e-21 94.0
Msa0593820 MtrunA17_Chr7g0270031 23.776 429 291 10 228 624 130 554 6.01e-21 97.1
Msa0593820 MtrunA17_Chr4g0057891 24.433 397 262 13 256 625 136 521 2.02e-20 95.1
Msa0593820 MtrunA17_Chr2g0328381 25.067 375 250 12 270 623 276 640 2.44e-19 92.4
Msa0593820 MtrunA17_Chr8g0383981 26.963 382 239 16 263 623 133 495 3.60e-19 91.3
Msa0593820 MtrunA17_Chr2g0294471 25.197 381 260 12 265 624 200 576 9.78e-19 90.1
Msa0593820 MtrunA17_Chr2g0328431 22.222 414 289 10 232 623 251 653 6.64e-18 87.8
Msa0593820 MtrunA17_Chr7g0238891 21.914 397 286 9 248 624 348 740 1.60e-17 86.7
Msa0593820 MtrunA17_Chr2g0294461 24.479 384 262 12 265 624 206 585 2.28e-17 85.9
Msa0593820 MtrunA17_Chr2g0328341 23.262 374 258 10 270 623 273 637 3.06e-17 85.5
Msa0593820 MtrunA17_Chr5g0401131 21.108 379 272 8 270 624 115 490 2.88e-16 82.0
Msa0593820 MtrunA17_Chr2g0328391 23.839 323 217 8 274 574 304 619 5.85e-16 81.6
Msa0593820 MtrunA17_Chr2g0328411 21.684 392 275 10 251 623 275 653 1.62e-15 80.1
Msa0593820 MtrunA17_Chr8g0357601 21.914 397 267 10 258 624 169 552 4.51e-15 78.6
Msa0593820 MtrunA17_Chr4g0031741 21.505 372 267 8 270 623 285 649 4.88e-15 78.6
Msa0593820 MtrunA17_Chr2g0328371 24.706 255 171 7 337 577 2 249 1.91e-14 73.6
Msa0593820 MtrunA17_Chr1g0194621 23.776 286 198 9 349 624 243 518 4.22e-14 75.5
Msa0593820 MtrunA17_Chr4g0031711 24.458 323 216 8 225 529 321 633 6.42e-14 75.1
Msa0593820 MtrunA17_Chr2g0328361 25.087 287 187 11 270 541 275 548 1.56e-13 73.9
Msa0593820 MtrunA17_Chr4g0031701 24.910 277 186 7 270 529 255 526 4.25e-13 72.4
Msa0593820 MtrunA17_Chr2g0329591 21.951 369 246 10 288 624 93 451 4.63e-13 71.6
Msa0593820 MtrunA17_Chr4g0031731 24.549 277 187 7 270 529 255 526 7.41e-13 71.6
Msa0593820 MtrunA17_Chr2g0328401 22.523 222 154 6 229 440 250 463 2.11e-12 70.1
Msa0593820 MtrunA17_Chr4g0031721 18.660 418 304 11 234 623 274 683 1.06e-11 68.2
Msa0593820 MtrunA17_Chr5g0396491 27.746 173 117 3 270 438 57 225 2.75e-11 66.2
Msa0593820 MtrunA17_Chr4g0038851 31.351 185 113 4 270 446 49 227 5.09e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0593820 AT4G00150.1 41.864 633 279 17 4 625 1 555 4.55e-134 404
Msa0593820 AT2G45160.1 39.435 672 325 24 4 625 1 640 1.01e-111 349
Msa0593820 AT3G60630.1 39.545 660 324 25 4 625 1 623 1.60e-109 343
Msa0593820 AT4G36710.1 32.447 376 221 11 268 624 124 485 1.47e-51 185
Msa0593820 AT2G04890.1 28.495 372 237 9 270 624 54 413 5.97e-38 145
Msa0593820 AT5G66770.1 29.091 385 250 12 256 624 207 584 1.48e-36 145
Msa0593820 AT2G01570.1 26.431 367 248 8 270 624 225 581 4.46e-30 125
Msa0593820 AT3G50650.1 27.649 387 239 12 262 624 173 542 5.40e-30 125
Msa0593820 AT5G17490.1 27.989 368 240 10 270 624 161 516 6.03e-30 124
Msa0593820 AT3G54220.1 27.198 364 239 7 274 623 298 649 7.73e-29 122
Msa0593820 AT4G17230.1 26.579 380 255 9 263 624 152 525 9.83e-29 121
Msa0593820 AT4G17230.2 26.579 380 255 9 263 624 152 525 9.83e-29 121
Msa0593820 AT3G03450.1 25.203 369 255 8 270 624 184 545 1.02e-28 121
Msa0593820 AT5G48150.1 23.607 377 271 8 263 624 116 490 4.10e-28 118
Msa0593820 AT5G48150.4 23.607 377 271 8 263 624 116 490 4.10e-28 118
Msa0593820 AT5G48150.2 23.607 377 271 8 263 624 116 490 4.10e-28 118
Msa0593820 AT5G48150.3 23.607 377 271 8 263 624 116 490 4.10e-28 118
Msa0593820 AT1G21450.1 25.135 370 260 7 270 624 226 593 4.33e-28 119
Msa0593820 AT1G14920.1 25.069 363 258 7 270 624 173 529 8.66e-28 118
Msa0593820 AT1G66350.1 25.000 368 248 10 270 625 156 507 1.14e-27 117
Msa0593820 AT1G50600.3 23.301 412 273 10 236 624 135 526 7.97e-27 115
Msa0593820 AT5G41920.1 25.401 374 243 9 270 624 44 400 8.44e-27 113
Msa0593820 AT1G50600.1 23.301 412 273 10 236 624 206 597 2.58e-26 114
Msa0593820 AT1G50600.2 23.301 412 273 10 236 624 210 601 2.87e-26 114
Msa0593820 AT1G63100.2 25.849 383 238 13 275 623 283 653 3.18e-22 101
Msa0593820 AT1G63100.1 25.849 383 238 13 275 623 258 628 4.33e-22 101
Msa0593820 AT1G55580.1 24.590 305 215 6 335 624 141 445 2.97e-19 91.3
Msa0593820 AT4G08250.1 26.246 301 203 10 337 623 181 476 3.72e-18 88.2
Msa0593820 AT5G59450.1 21.925 374 270 8 270 624 228 598 7.42e-18 87.8
Msa0593820 AT4G37650.1 20.573 384 275 9 270 624 147 529 3.16e-17 85.5
Msa0593820 AT3G46600.1 21.563 371 271 8 270 624 213 579 1.28e-14 77.4
Msa0593820 AT1G07530.1 20.290 414 311 10 229 623 351 764 1.94e-14 77.4
Msa0593820 AT3G46600.3 21.563 371 271 8 270 624 181 547 2.98e-14 76.3
Msa0593820 AT3G46600.2 21.984 373 267 9 270 624 5 371 4.40e-14 74.7
Msa0593820 AT1G07520.3 25.743 202 139 5 274 466 324 523 9.53e-14 75.1
Msa0593820 AT1G07520.1 25.743 202 139 5 274 466 323 522 9.61e-14 75.1
Msa0593820 AT1G07520.2 25.743 202 139 5 274 466 159 358 1.20e-13 74.3
Msa0593820 AT1G07520.4 25.743 202 139 5 274 466 159 358 1.20e-13 74.3
Msa0593820 AT1G50420.1 29.016 193 120 4 270 449 58 246 2.41e-12 70.1
Msa0593820 AT2G29065.1 21.180 373 269 9 274 623 257 627 1.25e-11 68.2
Msa0593820 AT2G29060.1 20.912 373 273 10 270 623 322 691 3.63e-11 66.6
Msa0593820 AT2G29060.2 20.912 373 273 10 270 623 285 654 3.85e-11 66.6

Find 158 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAAGAATCCTCTCTTTA+TGG 0.092963 4_2:+20374614 Msa0593820:three_prime_UTR
CCTAATGTTTGTTAACTAAA+TGG 0.126757 4_2:+20374445 Msa0593820:three_prime_UTR
TTACACAACAATACACTTTA+AGG 0.131559 4_2:+20371987 Msa0593820:five_prime_UTR
AAAATAAAGATTGAGTATTT+TGG 0.160504 4_2:-20371710 None:intergenic
AATTGCGTGCTGAGATATTT+TGG 0.187631 4_2:-20371801 None:intergenic
TTACAATGTGTTACCGCTTT+TGG 0.235424 4_2:+20373043 Msa0593820:CDS
GTCTTGTCTTTCTTGATCTA+AGG 0.247486 4_2:+20374641 Msa0593820:three_prime_UTR
TGTAATGCCTTTCAATCTTT+AGG 0.257542 4_2:-20374066 None:intergenic
GATAAGTGGCTAATTATTAA+AGG 0.259137 4_2:+20374335 Msa0593820:exon
TAGTAAGAAATGAACAATTT+AGG 0.267323 4_2:+20374586 Msa0593820:three_prime_UTR
AGACAAGGCATAGTAATTTA+TGG 0.269109 4_2:-20371575 None:intergenic
TGTGGAGCCCACTTCAGTTC+TGG 0.271815 4_2:+20372614 Msa0593820:CDS
TTGAAATTCCTCAAAAGTTA+AGG 0.300516 4_2:-20372472 None:intergenic
CCATTTAGTTAACAAACATT+AGG 0.311269 4_2:-20374445 None:intergenic
CATGTTATTGATTTCGATAT+CGG 0.319001 4_2:+20373572 Msa0593820:CDS
GACAATGTTGAAGATGAAAA+TGG 0.331833 4_2:-20372660 None:intergenic
TCCAAAGTTACGACCACTTT+CGG 0.339309 4_2:-20373929 None:intergenic
TTAAAGGTTGAAATTTCTTA+AGG 0.345571 4_2:+20374351 Msa0593820:three_prime_UTR
ATTGTTTACAGAAACTGATT+TGG 0.353647 4_2:-20371607 None:intergenic
TGATGGATTACTTATGAAAC+TGG 0.357205 4_2:-20373867 None:intergenic
CATTTAGTTAACAAACATTA+GGG 0.374065 4_2:-20374444 None:intergenic
TCTTGATCTAAGGCTGTGTT+TGG 0.374269 4_2:+20374651 Msa0593820:three_prime_UTR
ATTGATTTCGATATCGGGTT+TGG 0.379458 4_2:+20373578 Msa0593820:CDS
CTAATGTTTGTTAACTAAAT+GGG 0.381359 4_2:+20374446 Msa0593820:three_prime_UTR
ACAATAATAGAAACTAATTA+TGG 0.382308 4_2:-20371750 None:intergenic
TGAAATTCCTCAAAAGTTAA+GGG 0.383228 4_2:-20372471 None:intergenic
GGTTCAAGCGCAAGGGATAT+TGG 0.384808 4_2:+20373304 Msa0593820:CDS
ATCAACCTCAGCAACAAAAT+TGG 0.386235 4_2:+20372565 Msa0593820:CDS
ACTAATTATGGTTATGATAA+GGG 0.386415 4_2:-20371738 None:intergenic
TAAACACTCGATCGAGTGTT+TGG 0.388594 4_2:+20374705 Msa0593820:three_prime_UTR
CTTCCACTCTTGTGCTATGC+TGG 0.398096 4_2:+20374272 Msa0593820:CDS
TGAATGAAGCATTAACTGTA+AGG 0.398966 4_2:-20373396 None:intergenic
AACTAATTATGGTTATGATA+AGG 0.416365 4_2:-20371739 None:intergenic
GGAAGCTCAAGCCGAGTGTT+TGG 0.423122 4_2:+20374207 Msa0593820:CDS
AATTAGCTGTAATTTGTGTT+TGG 0.426950 4_2:+20374759 Msa0593820:three_prime_UTR
CAAATATACTCTGCTGAAGC+TGG 0.434387 4_2:-20373238 None:intergenic
ATTTCAAGTGAAGGGAATGT+TGG 0.436223 4_2:+20372488 Msa0593820:CDS
TTACTTATGAAACTGGAAAC+AGG 0.436478 4_2:-20373860 None:intergenic
AATATCCCTTGCGCTTGAAC+CGG 0.438162 4_2:-20373302 None:intergenic
TCAAGCCCTCATTGAAGCCT+TGG 0.443453 4_2:+20373532 Msa0593820:CDS
GCCGAAAGTGGTCGTAACTT+TGG 0.444136 4_2:+20373928 Msa0593820:CDS
CTTCACATTCAGCAACTTCA+CGG 0.445110 4_2:+20374186 Msa0593820:CDS
CTTTGAAAAGGCTTACCAAT+TGG 0.446191 4_2:-20373350 None:intergenic
ATGTTATTGATTTCGATATC+GGG 0.446925 4_2:+20373573 Msa0593820:CDS
TTGTAATTCTCAGGAATGAA+TGG 0.453236 4_2:-20373026 None:intergenic
GAATGTGAAGGGAGAGAAAC+CGG 0.460030 4_2:-20374173 None:intergenic
GGTAACACATTGTAATTCTC+AGG 0.461882 4_2:-20373035 None:intergenic
AAGCCGAGTGTTTGGTTCAA+AGG 0.464484 4_2:+20374215 Msa0593820:CDS
TGGTATGAGAATGGAGGATT+GGG 0.465608 4_2:+20372803 Msa0593820:CDS
TAGAAAGAAGCCCTTTGAAA+AGG 0.471306 4_2:-20373362 None:intergenic
AGCCGAGTGTTTGGTTCAAA+GGG 0.474056 4_2:+20374216 Msa0593820:CDS
GAAGATGAAAATGGAGAACT+AGG 0.474745 4_2:-20372651 None:intergenic
AAGGTTGAAATTTCTTAAGG+TGG 0.475998 4_2:+20374354 Msa0593820:three_prime_UTR
TGGGTTGAACATGGACGCTT+CGG 0.477095 4_2:+20372977 Msa0593820:CDS
AAAGAACAAGGACAAAGCTT+AGG 0.481534 4_2:-20371640 None:intergenic
AAGTTGGCAAACTGAAGAAC+GGG 0.484332 4_2:-20373500 None:intergenic
AATCACCAGCTCTCACCAAT+TGG 0.485962 4_2:+20373335 Msa0593820:CDS
AAGGGCTTCTTTCTACATGA+AGG 0.487466 4_2:+20373370 Msa0593820:CDS
CTTGCCATCATGTCCTCTAC+CGG 0.490539 4_2:+20373796 Msa0593820:CDS
GGCTTGATTACAAGTAAAGT+TGG 0.491191 4_2:-20373516 None:intergenic
CAGTTAATGCTTCATTCAAA+CGG 0.491869 4_2:+20373401 Msa0593820:CDS
TGGTGGTGGTGGTATGAGAA+TGG 0.492769 4_2:+20372794 Msa0593820:CDS
TGCTCAGATATCAATGTGGC+AGG 0.493264 4_2:-20371663 None:intergenic
AACTCATCTCAATTTCAACT+TGG 0.495720 4_2:+20374305 Msa0593820:CDS
TTTGAGGAATTTCAAGTGAA+GGG 0.497110 4_2:+20372480 Msa0593820:CDS
GCTGATAGAAGCTGGTAATC+CGG 0.498189 4_2:+20373283 Msa0593820:CDS
AGGAACATGTTTCTTCAGTC+CGG 0.498389 4_2:+20374154 Msa0593820:CDS
CCTTTCCAAGGCTTCAATGA+GGG 0.498450 4_2:-20373537 None:intergenic
GTGGTATGAGAATGGAGGAT+TGG 0.504718 4_2:+20372802 Msa0593820:CDS
TTCAAATAGCAATACTTCAA+TGG 0.504902 4_2:+20372716 Msa0593820:CDS
TTTGATAGTAATGAAGCCAT+TGG 0.505073 4_2:+20373827 Msa0593820:CDS
GTAATTTGTCACGCTGGTTG+TGG 0.506339 4_2:-20374123 None:intergenic
TCCAATTGCGAAGCAGCAAA+AGG 0.508239 4_2:+20373181 Msa0593820:CDS
GATGTTGAAGACCCTTATGC+TGG 0.508840 4_2:+20372864 Msa0593820:CDS
CAACCGACGACTGCTGTTGT+TGG 0.513401 4_2:-20373157 None:intergenic
AACTTGGAGATGTTGATAAG+TGG 0.518300 4_2:+20374321 Msa0593820:exon
TCCACTTGAAATCCTCTCAC+AGG 0.520356 4_2:-20374241 None:intergenic
TCTTGAATCCAGAACTGAAG+TGG 0.526232 4_2:-20372622 None:intergenic
CTGGTCAAGAAGTGTTTGCT+AGG 0.526401 4_2:+20373069 Msa0593820:CDS
TTCAATCTCGTTGCAAGATG+AGG 0.530780 4_2:-20373684 None:intergenic
TATGATGTCTCTTTGAACAT+TGG 0.534220 4_2:+20374502 Msa0593820:three_prime_UTR
CTGGATGCTTGCATCATCTC+CGG 0.537282 4_2:-20373219 None:intergenic
TGAACGACATTGGTGGGAAG+AGG 0.544276 4_2:-20373977 None:intergenic
TTGTCATCTTCTCATGGTAG+TGG 0.544818 4_2:+20372678 Msa0593820:CDS
ATCTTGCAAAGTGGTGGTTG+TGG 0.551252 4_2:+20372897 Msa0593820:CDS
TGCTAGGAGACATCAGCAGC+AGG 0.551740 4_2:+20373085 Msa0593820:CDS
GGTAATCCGGTTCAAGCGCA+AGG 0.552039 4_2:+20373296 Msa0593820:CDS
TCAACATTGTCATCTTCTCA+TGG 0.552805 4_2:+20372672 Msa0593820:CDS
TGTTTGTTAACTAAATGGGT+TGG 0.553496 4_2:+20374450 Msa0593820:three_prime_UTR
AATTCGATCGAACCTTTCCA+AGG 0.555850 4_2:-20373549 None:intergenic
GGTAGTGGTAGTAATGGTAA+TGG 0.556024 4_2:+20372693 Msa0593820:CDS
ACAACAACATGGGTTGAACA+TGG 0.557271 4_2:+20372968 Msa0593820:CDS
GCTTACCAATTGGTGAGAGC+TGG 0.557459 4_2:-20373340 None:intergenic
AAGGTGAGTTCTAGTACTAC+CGG 0.560768 4_2:+20373200 Msa0593820:CDS
CTCTTGTGCTATGCTGGCAG+AGG 0.560950 4_2:+20374278 Msa0593820:CDS
CCCTCATTGAAGCCTTGGAA+AGG 0.561376 4_2:+20373537 Msa0593820:CDS
ACTGCTGAGCTGATAGAAGC+TGG 0.561856 4_2:+20373275 Msa0593820:CDS
ATGTACTCCTAAAGATTGAA+AGG 0.562335 4_2:+20374059 Msa0593820:CDS
AAAGCAGCTAAGGCCGAAAG+TGG 0.562649 4_2:+20373916 Msa0593820:CDS
GTTCAAAGGGCACCTGTGAG+AGG 0.570717 4_2:+20374229 Msa0593820:CDS
AGAGCCACAGGGAAGCATGA+TGG 0.571215 4_2:-20373884 None:intergenic
CATCATTTGAAAGATGAGCA+AGG 0.573001 4_2:-20372752 None:intergenic
TCATGATGATGAGGAAACAA+TGG 0.573020 4_2:-20373119 None:intergenic
TCTCATGGTAGTGGTAGTAA+TGG 0.576556 4_2:+20372687 Msa0593820:CDS
ACCATCAACAAAATCATCCT+CGG 0.579390 4_2:+20374097 Msa0593820:CDS
TCAATCTCGTTGCAAGATGA+GGG 0.580762 4_2:-20373683 None:intergenic
TGAAGAAACATGTTCCTCCA+TGG 0.581165 4_2:-20374148 None:intergenic
CAGCATAGCACAAGAGTGGA+AGG 0.583062 4_2:-20374271 None:intergenic
TCAAAGAAATGACCCGGTAG+AGG 0.583745 4_2:-20373809 None:intergenic
AGAACATGAACGACATTGGT+GGG 0.584347 4_2:-20373983 None:intergenic
ACCTTTCCAAGGCTTCAATG+AGG 0.587226 4_2:-20373538 None:intergenic
AACAAAATCTTGCAAAGTGG+TGG 0.589673 4_2:+20372891 Msa0593820:CDS
AAGAACATGAACGACATTGG+TGG 0.589769 4_2:-20373984 None:intergenic
TGGGAAGAGGAACATCCATT+CGG 0.591209 4_2:-20373964 None:intergenic
TTGGATCATCAACAACAACA+TGG 0.592763 4_2:+20372957 Msa0593820:CDS
TTCAAACGGTAACAATCTCA+CGG 0.592949 4_2:+20373415 Msa0593820:CDS
AACAGGAAAGTTCACACCAA+TGG 0.593516 4_2:-20373843 None:intergenic
CTTGAATCCAGAACTGAAGT+GGG 0.594064 4_2:-20372621 None:intergenic
TTGAAGAACATGAACGACAT+TGG 0.594485 4_2:-20373987 None:intergenic
AGCGTGACAAATTACCTCCA+TGG 0.597025 4_2:+20374131 Msa0593820:CDS
GAGATTGCACTAAGAAGTAA+CGG 0.597858 4_2:+20373626 Msa0593820:CDS
AAAGTTGGCAAACTGAAGAA+CGG 0.598516 4_2:-20373501 None:intergenic
GATAACAGAGAAACAGTGAA+AGG 0.602580 4_2:-20374543 None:intergenic
TGGATCATCAACAACAACAT+GGG 0.602702 4_2:+20372958 Msa0593820:CDS
CGTGAAGTTGCTGAATGTGA+AGG 0.606027 4_2:-20374185 None:intergenic
CTGCCAGCATAGCACAAGAG+TGG 0.610500 4_2:-20374275 None:intergenic
TGGTGGTGGTATGAGAATGG+AGG 0.610885 4_2:+20372797 Msa0593820:CDS
TAAAGAAGATTATTGCTATG+TGG 0.612360 4_2:+20372596 Msa0593820:CDS
TTGCCATCATGTCCTCTACC+GGG 0.614713 4_2:+20373797 Msa0593820:CDS
TGACCCGGTAGAGGACATGA+TGG 0.615427 4_2:-20373800 None:intergenic
TGAACTTAAACGACATGTTG+AGG 0.620897 4_2:-20373748 None:intergenic
CAATGTGGCAGGAAAGAACA+AGG 0.624770 4_2:-20371652 None:intergenic
ATGAGAATGGAGGATTGGGA+AGG 0.628687 4_2:+20372807 Msa0593820:CDS
ATGGAGGTAATTTGTCACGC+TGG 0.629161 4_2:-20374129 None:intergenic
AAGATGAAAATGGAGAACTA+GGG 0.633076 4_2:-20372650 None:intergenic
ACAGCAGTAATCTTAAGTGA+AGG 0.636223 4_2:-20373653 None:intergenic
GTAATCCGGTTCAAGCGCAA+GGG 0.638818 4_2:+20373297 Msa0593820:CDS
CAAGACTAAACCATAAAGAG+AGG 0.643558 4_2:-20374624 None:intergenic
AAGCGCAAGGGATATTGGCG+CGG 0.648472 4_2:+20373309 Msa0593820:CDS
AGATGGATGCGTGCATACGT+AGG 0.650132 4_2:-20371830 None:intergenic
TGCCCTTTGAACCAAACACT+CGG 0.651926 4_2:-20374218 None:intergenic
ACCTGTGAGAGGATTTCAAG+TGG 0.654432 4_2:+20374240 Msa0593820:CDS
TGCTGCAGATCATGATGATG+AGG 0.657062 4_2:-20373128 None:intergenic
GTGAAGTTGCTGAATGTGAA+GGG 0.658589 4_2:-20374184 None:intergenic
ATGGACGCTTCGGTTCAACA+AGG 0.660128 4_2:+20372987 Msa0593820:CDS
ATATCGGGTTTGGAGTACAA+TGG 0.666838 4_2:+20373588 Msa0593820:CDS
TTACTATCAAAGAAATGACC+CGG 0.669847 4_2:-20373815 None:intergenic
CACGCTGGTTGTGGTGACCG+AGG 0.670125 4_2:-20374114 None:intergenic
TCTTGACCAGAATCCAAAAG+CGG 0.685854 4_2:-20373056 None:intergenic
TGGACGCTTCGGTTCAACAA+GGG 0.687015 4_2:+20372988 Msa0593820:CDS
ATTTGGACATGTCAGAGACA+AGG 0.688777 4_2:-20371590 None:intergenic
TTACCAACAACAGCAGTCGT+CGG 0.697756 4_2:+20373154 Msa0593820:CDS
AGAAACATGTTCCTCCATGG+AGG 0.701239 4_2:-20374145 None:intergenic
TAATCCATCATGCTTCCCTG+TGG 0.706732 4_2:+20373880 Msa0593820:CDS
CAATCTTTAGGAGTACATCG+AGG 0.714128 4_2:-20374054 None:intergenic
TTGAACAAAATCTTGCAAAG+TGG 0.719051 4_2:+20372888 Msa0593820:CDS
AAAATGCTCAGATATCAATG+TGG 0.751076 4_2:-20371667 None:intergenic
GTGACAAATTACCTCCATGG+AGG 0.762900 4_2:+20374134 Msa0593820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGATGATTTTTTTTATT+GGG - chr4_2:20372132-20372151 None:intergenic 10.0%
!!! AAAAAGATGATTTTTTTTAT+TGG - chr4_2:20372133-20372152 None:intergenic 10.0%
!!! AAAATAAAGATTGAGTATTT+TGG - chr4_2:20371713-20371732 None:intergenic 15.0%
!! ACAATAATAGAAACTAATTA+TGG - chr4_2:20371753-20371772 None:intergenic 15.0%
!!! AAAGATGATTTTTTTTATTG+GGG - chr4_2:20372131-20372150 None:intergenic 15.0%
!!! AAAATCATCTTTTTTTCATA+AGG + chr4_2:20372139-20372158 Msa0593820:intron 15.0%
!!! AAATCATCTTTTTTTCATAA+GGG + chr4_2:20372140-20372159 Msa0593820:intron 15.0%
!!! ATTTTGAAAATGAAACTAAT+CGG - chr4_2:20373449-20373468 None:intergenic 15.0%
!!! ATTAGTTTCATTTTCAAAAT+TGG + chr4_2:20373449-20373468 Msa0593820:CDS 15.0%
!!! ATTGTTTGTGTAATTTTTTT+AGG + chr4_2:20374402-20374421 Msa0593820:three_prime_UTR 15.0%
!! ACTAATTATGGTTATGATAA+GGG - chr4_2:20371741-20371760 None:intergenic 20.0%
!! AACTAATTATGGTTATGATA+AGG - chr4_2:20371742-20371761 None:intergenic 20.0%
!!! TTTTTTTTATTGGGGTATTA+GGG - chr4_2:20372123-20372142 None:intergenic 20.0%
!!! ATTTTTTTTATTGGGGTATT+AGG - chr4_2:20372124-20372143 None:intergenic 20.0%
!!! TTGAAATTAAATCTGTTTTC+TGG + chr4_2:20372274-20372293 Msa0593820:intron 20.0%
!!! TGAAATTAAATCTGTTTTCT+GGG + chr4_2:20372275-20372294 Msa0593820:intron 20.0%
!!! TTGTTTTTGTTTTTAGATGA+AGG + chr4_2:20372443-20372462 Msa0593820:intron 20.0%
!! TTAAAGGTTGAAATTTCTTA+AGG + chr4_2:20374351-20374370 Msa0593820:three_prime_UTR 20.0%
!! CATTTAGTTAACAAACATTA+GGG - chr4_2:20374447-20374466 None:intergenic 20.0%
!!! CTAATGTTTGTTAACTAAAT+GGG + chr4_2:20374446-20374465 Msa0593820:three_prime_UTR 20.0%
!! TAGTAAGAAATGAACAATTT+AGG + chr4_2:20374586-20374605 Msa0593820:three_prime_UTR 20.0%
! ATTGTTTACAGAAACTGATT+TGG - chr4_2:20371610-20371629 None:intergenic 25.0%
! TTACACAACAATACACTTTA+AGG + chr4_2:20371987-20372006 Msa0593820:five_prime_UTR 25.0%
!!! TTTTTTCCTTTTAGCCAAAT+GGG - chr4_2:20372230-20372249 None:intergenic 25.0%
!!! TTTTTTTCCTTTTAGCCAAA+TGG - chr4_2:20372231-20372250 None:intergenic 25.0%
!! GAAATTAAATCTGTTTTCTG+GGG + chr4_2:20372276-20372295 Msa0593820:intron 25.0%
! TTCTTTGTTCTTGTTCAAAT+TGG + chr4_2:20372409-20372428 Msa0593820:intron 25.0%
! TGAAATTCCTCAAAAGTTAA+GGG - chr4_2:20372474-20372493 None:intergenic 25.0%
! TTGAAATTCCTCAAAAGTTA+AGG - chr4_2:20372475-20372494 None:intergenic 25.0%
! TAAAGAAGATTATTGCTATG+TGG + chr4_2:20372596-20372615 Msa0593820:CDS 25.0%
! TTCAAATAGCAATACTTCAA+TGG + chr4_2:20372716-20372735 Msa0593820:CDS 25.0%
!!! TCACTTTTGAGGTTAATTAT+GGG + chr4_2:20372840-20372859 Msa0593820:CDS 25.0%
!!! TCTCAAAATGTTGATTTTCA+TGG + chr4_2:20372921-20372940 Msa0593820:CDS 25.0%
!!! CATGTTATTGATTTCGATAT+CGG + chr4_2:20373572-20373591 Msa0593820:CDS 25.0%
!!! ATGTTATTGATTTCGATATC+GGG + chr4_2:20373573-20373592 Msa0593820:CDS 25.0%
!!! TTCATTTTTTAAAGCAGCTA+AGG + chr4_2:20373906-20373925 Msa0593820:CDS 25.0%
! GATAAGTGGCTAATTATTAA+AGG + chr4_2:20374335-20374354 Msa0593820:exon 25.0%
! CCATTTAGTTAACAAACATT+AGG - chr4_2:20374448-20374467 None:intergenic 25.0%
!! CCTAATGTTTGTTAACTAAA+TGG + chr4_2:20374445-20374464 Msa0593820:three_prime_UTR 25.0%
! AATTAAGAATCCTCTCTTTA+TGG + chr4_2:20374614-20374633 Msa0593820:three_prime_UTR 25.0%
AGACAAGGCATAGTAATTTA+TGG - chr4_2:20371578-20371597 None:intergenic 30.0%
AAAATGCTCAGATATCAATG+TGG - chr4_2:20371670-20371689 None:intergenic 30.0%
!! GTGTTGTGTTTTTGTTAAGA+TGG - chr4_2:20371850-20371869 None:intergenic 30.0%
! AAAACAAGTACAAGTTGAGT+AGG - chr4_2:20372015-20372034 None:intergenic 30.0%
! GAACAAAGGTGTAAACTTTA+TGG + chr4_2:20372331-20372350 Msa0593820:intron 30.0%
!! TTTGAGACTTTTGAGTCATA+TGG + chr4_2:20372357-20372376 Msa0593820:intron 30.0%
!! TTGAGACTTTTGAGTCATAT+GGG + chr4_2:20372358-20372377 Msa0593820:intron 30.0%
!! TTTTGAGTCATATGGGTTAT+TGG + chr4_2:20372365-20372384 Msa0593820:intron 30.0%
!! TTTTGAGGAATTTCAAGTGA+AGG + chr4_2:20372479-20372498 Msa0593820:CDS 30.0%
! TTTGAGGAATTTCAAGTGAA+GGG + chr4_2:20372480-20372499 Msa0593820:CDS 30.0%
AAGATGAAAATGGAGAACTA+GGG - chr4_2:20372653-20372672 None:intergenic 30.0%
GACAATGTTGAAGATGAAAA+TGG - chr4_2:20372663-20372682 None:intergenic 30.0%
GAGAATAGTGAAGAAAAATG+TGG + chr4_2:20372774-20372793 Msa0593820:CDS 30.0%
AATAGTGAAGAAAAATGTGG+TGG + chr4_2:20372777-20372796 Msa0593820:CDS 30.0%
!!! GTCACTTTTGAGGTTAATTA+TGG + chr4_2:20372839-20372858 Msa0593820:CDS 30.0%
! TTTTGTTCAATCCAGCATAA+GGG - chr4_2:20372878-20372897 None:intergenic 30.0%
! ATTTTGTTCAATCCAGCATA+AGG - chr4_2:20372879-20372898 None:intergenic 30.0%
TTGAACAAAATCTTGCAAAG+TGG + chr4_2:20372888-20372907 Msa0593820:CDS 30.0%
!!! CAAAATGTTGATTTTCATGG+TGG + chr4_2:20372924-20372943 Msa0593820:CDS 30.0%
TTGTAATTCTCAGGAATGAA+TGG - chr4_2:20373029-20373048 None:intergenic 30.0%
! AATTGGTAAGCCTTTTCAAA+GGG + chr4_2:20373352-20373371 Msa0593820:CDS 30.0%
! TGAATGAAGCATTAACTGTA+AGG - chr4_2:20373399-20373418 None:intergenic 30.0%
CAGTTAATGCTTCATTCAAA+CGG + chr4_2:20373401-20373420 Msa0593820:CDS 30.0%
TTACTATCAAAGAAATGACC+CGG - chr4_2:20373818-20373837 None:intergenic 30.0%
! TTTGATAGTAATGAAGCCAT+TGG + chr4_2:20373827-20373846 Msa0593820:CDS 30.0%
TTACTTATGAAACTGGAAAC+AGG - chr4_2:20373863-20373882 None:intergenic 30.0%
!! TGATGGATTACTTATGAAAC+TGG - chr4_2:20373870-20373889 None:intergenic 30.0%
TTAAAAAATGAAGAGCCACA+GGG - chr4_2:20373898-20373917 None:intergenic 30.0%
TTTAAAAAATGAAGAGCCAC+AGG - chr4_2:20373899-20373918 None:intergenic 30.0%
ATGTACTCCTAAAGATTGAA+AGG + chr4_2:20374059-20374078 Msa0593820:CDS 30.0%
TGTAATGCCTTTCAATCTTT+AGG - chr4_2:20374069-20374088 None:intergenic 30.0%
AACTCATCTCAATTTCAACT+TGG + chr4_2:20374305-20374324 Msa0593820:CDS 30.0%
AAGGTTGAAATTTCTTAAGG+TGG + chr4_2:20374354-20374373 Msa0593820:three_prime_UTR 30.0%
! TGTTTGTTAACTAAATGGGT+TGG + chr4_2:20374450-20374469 Msa0593820:three_prime_UTR 30.0%
TATGATGTCTCTTTGAACAT+TGG + chr4_2:20374502-20374521 Msa0593820:three_prime_UTR 30.0%
AAAGAACAAGGACAAAGCTT+AGG - chr4_2:20371643-20371662 None:intergenic 35.0%
! AATTGCGTGCTGAGATATTT+TGG - chr4_2:20371804-20371823 None:intergenic 35.0%
ATCAAGATTCAAGACCCATT+TGG + chr4_2:20372213-20372232 Msa0593820:intron 35.0%
!!! CATATGGGTTATTGGTTTTG+TGG + chr4_2:20372373-20372392 Msa0593820:intron 35.0%
! ATTTCAAGTGAAGGGAATGT+TGG + chr4_2:20372488-20372507 Msa0593820:CDS 35.0%
ATCAACCTCAGCAACAAAAT+TGG + chr4_2:20372565-20372584 Msa0593820:CDS 35.0%
! AATGACCAATTTTGTTGCTG+AGG - chr4_2:20372573-20372592 None:intergenic 35.0%
GAAGATGAAAATGGAGAACT+AGG - chr4_2:20372654-20372673 None:intergenic 35.0%
TCAACATTGTCATCTTCTCA+TGG + chr4_2:20372672-20372691 Msa0593820:CDS 35.0%
CATCATTTGAAAGATGAGCA+AGG - chr4_2:20372755-20372774 None:intergenic 35.0%
AACAAAATCTTGCAAAGTGG+TGG + chr4_2:20372891-20372910 Msa0593820:CDS 35.0%
!! GTTGATTTTCATGGTGGTTT+TGG + chr4_2:20372930-20372949 Msa0593820:CDS 35.0%
TTGGATCATCAACAACAACA+TGG + chr4_2:20372957-20372976 Msa0593820:CDS 35.0%
TGGATCATCAACAACAACAT+GGG + chr4_2:20372958-20372977 Msa0593820:CDS 35.0%
GGTAACACATTGTAATTCTC+AGG - chr4_2:20373038-20373057 None:intergenic 35.0%
! TTACAATGTGTTACCGCTTT+TGG + chr4_2:20373043-20373062 Msa0593820:CDS 35.0%
! TCATGATGATGAGGAAACAA+TGG - chr4_2:20373122-20373141 None:intergenic 35.0%
! CTTTGAAAAGGCTTACCAAT+TGG - chr4_2:20373353-20373372 None:intergenic 35.0%
! CAATTGGTAAGCCTTTTCAA+AGG + chr4_2:20373351-20373370 Msa0593820:CDS 35.0%
! TAGAAAGAAGCCCTTTGAAA+AGG - chr4_2:20373365-20373384 None:intergenic 35.0%
TTCAAACGGTAACAATCTCA+CGG + chr4_2:20373415-20373434 Msa0593820:CDS 35.0%
AAAGTTGGCAAACTGAAGAA+CGG - chr4_2:20373504-20373523 None:intergenic 35.0%
GGCTTGATTACAAGTAAAGT+TGG - chr4_2:20373519-20373538 None:intergenic 35.0%
! ATTGATTTCGATATCGGGTT+TGG + chr4_2:20373578-20373597 Msa0593820:CDS 35.0%
GAGATTGCACTAAGAAGTAA+CGG + chr4_2:20373626-20373645 Msa0593820:CDS 35.0%
ACAGCAGTAATCTTAAGTGA+AGG - chr4_2:20373656-20373675 None:intergenic 35.0%
TGAACTTAAACGACATGTTG+AGG - chr4_2:20373751-20373770 None:intergenic 35.0%
TTGAAGAACATGAACGACAT+TGG - chr4_2:20373990-20374009 None:intergenic 35.0%
!!! TGATTTTGTTGATGGTTGGT+TGG - chr4_2:20374093-20374112 None:intergenic 35.0%
!!! AGGATGATTTTGTTGATGGT+TGG - chr4_2:20374097-20374116 None:intergenic 35.0%
ACCATCAACAAAATCATCCT+CGG + chr4_2:20374097-20374116 Msa0593820:CDS 35.0%
! AACTTGGAGATGTTGATAAG+TGG + chr4_2:20374321-20374340 Msa0593820:exon 35.0%
GATAACAGAGAAACAGTGAA+AGG - chr4_2:20374546-20374565 None:intergenic 35.0%
CAAGACTAAACCATAAAGAG+AGG - chr4_2:20374627-20374646 None:intergenic 35.0%
GTCTTGTCTTTCTTGATCTA+AGG + chr4_2:20374641-20374660 Msa0593820:three_prime_UTR 35.0%
ATTTGGACATGTCAGAGACA+AGG - chr4_2:20371593-20371612 None:intergenic 40.0%
TCAAGACCCATTTGGCTAAA+AGG + chr4_2:20372221-20372240 Msa0593820:intron 40.0%
!!! AAATCTGTTTTCTGGGGTAG+TGG + chr4_2:20372282-20372301 Msa0593820:intron 40.0%
AGAGGGTAAGAAGAGAACAA+AGG + chr4_2:20372317-20372336 Msa0593820:intron 40.0%
CTTGAATCCAGAACTGAAGT+GGG - chr4_2:20372624-20372643 None:intergenic 40.0%
TCTTGAATCCAGAACTGAAG+TGG - chr4_2:20372625-20372644 None:intergenic 40.0%
TTGTCATCTTCTCATGGTAG+TGG + chr4_2:20372678-20372697 Msa0593820:CDS 40.0%
!! TCTCATGGTAGTGGTAGTAA+TGG + chr4_2:20372687-20372706 Msa0593820:CDS 40.0%
! GGTAGTGGTAGTAATGGTAA+TGG + chr4_2:20372693-20372712 Msa0593820:CDS 40.0%
AGTGAAGAAAAATGTGGTGG+TGG + chr4_2:20372780-20372799 Msa0593820:CDS 40.0%
TGGTATGAGAATGGAGGATT+GGG + chr4_2:20372803-20372822 Msa0593820:CDS 40.0%
!!! GTCAAGATCAGTCACTTTTG+AGG + chr4_2:20372829-20372848 Msa0593820:CDS 40.0%
!!! TCATGGTGGTTTTGGTGTTT+TGG + chr4_2:20372938-20372957 Msa0593820:CDS 40.0%
! ACAACAACATGGGTTGAACA+TGG + chr4_2:20372968-20372987 Msa0593820:CDS 40.0%
TCTTGACCAGAATCCAAAAG+CGG - chr4_2:20373059-20373078 None:intergenic 40.0%
! AAGGTGAGTTCTAGTACTAC+CGG + chr4_2:20373200-20373219 Msa0593820:CDS 40.0%
CAAATATACTCTGCTGAAGC+TGG - chr4_2:20373241-20373260 None:intergenic 40.0%
AAGGGCTTCTTTCTACATGA+AGG + chr4_2:20373370-20373389 Msa0593820:CDS 40.0%
AAGTTGGCAAACTGAAGAAC+GGG - chr4_2:20373503-20373522 None:intergenic 40.0%
AATTCGATCGAACCTTTCCA+AGG - chr4_2:20373552-20373571 None:intergenic 40.0%
! ATATCGGGTTTGGAGTACAA+TGG + chr4_2:20373588-20373607 Msa0593820:CDS 40.0%
TCAATCTCGTTGCAAGATGA+GGG - chr4_2:20373686-20373705 None:intergenic 40.0%
TTCAATCTCGTTGCAAGATG+AGG - chr4_2:20373687-20373706 None:intergenic 40.0%
AACAGGAAAGTTCACACCAA+TGG - chr4_2:20373846-20373865 None:intergenic 40.0%
TCCAAAGTTACGACCACTTT+CGG - chr4_2:20373932-20373951 None:intergenic 40.0%
AGAACATGAACGACATTGGT+GGG - chr4_2:20373986-20374005 None:intergenic 40.0%
AAGAACATGAACGACATTGG+TGG - chr4_2:20373987-20374006 None:intergenic 40.0%
! CAATCTTTAGGAGTACATCG+AGG - chr4_2:20374057-20374076 None:intergenic 40.0%
! ACCGAGGATGATTTTGTTGA+TGG - chr4_2:20374101-20374120 None:intergenic 40.0%
TGAAGAAACATGTTCCTCCA+TGG - chr4_2:20374151-20374170 None:intergenic 40.0%
AGGAACATGTTTCTTCAGTC+CGG + chr4_2:20374154-20374173 Msa0593820:CDS 40.0%
GTGAAGTTGCTGAATGTGAA+GGG - chr4_2:20374187-20374206 None:intergenic 40.0%
CTTCACATTCAGCAACTTCA+CGG + chr4_2:20374186-20374205 Msa0593820:CDS 40.0%
! TCTTGATCTAAGGCTGTGTT+TGG + chr4_2:20374651-20374670 Msa0593820:three_prime_UTR 40.0%
TAAACACTCGATCGAGTGTT+TGG + chr4_2:20374705-20374724 Msa0593820:three_prime_UTR 40.0%
CAATGTGGCAGGAAAGAACA+AGG - chr4_2:20371655-20371674 None:intergenic 45.0%
TGCTCAGATATCAATGTGGC+AGG - chr4_2:20371666-20371685 None:intergenic 45.0%
!!! TTCTGGGGTAGTGGATTTTG+TGG + chr4_2:20372291-20372310 Msa0593820:intron 45.0%
!!! TCTGGGGTAGTGGATTTTGT+GGG + chr4_2:20372292-20372311 Msa0593820:intron 45.0%
!!! TAGTGGATTTTGTGGGGAAG+AGG + chr4_2:20372299-20372318 Msa0593820:intron 45.0%
!! AGTGGATTTTGTGGGGAAGA+GGG + chr4_2:20372300-20372319 Msa0593820:intron 45.0%
! GGCTTTGCCCTTAACTTTTG+AGG + chr4_2:20372464-20372483 Msa0593820:CDS 45.0%
!! GAAGAAAAATGTGGTGGTGG+TGG + chr4_2:20372783-20372802 Msa0593820:CDS 45.0%
GTGGTATGAGAATGGAGGAT+TGG + chr4_2:20372802-20372821 Msa0593820:CDS 45.0%
ATGAGAATGGAGGATTGGGA+AGG + chr4_2:20372807-20372826 Msa0593820:CDS 45.0%
GATGTTGAAGACCCTTATGC+TGG + chr4_2:20372864-20372883 Msa0593820:CDS 45.0%
ATCTTGCAAAGTGGTGGTTG+TGG + chr4_2:20372897-20372916 Msa0593820:CDS 45.0%
!! GTGTTACCGCTTTTGGATTC+TGG + chr4_2:20373050-20373069 Msa0593820:CDS 45.0%
!! CTGGTCAAGAAGTGTTTGCT+AGG + chr4_2:20373069-20373088 Msa0593820:CDS 45.0%
! TGCTGCAGATCATGATGATG+AGG - chr4_2:20373131-20373150 None:intergenic 45.0%
TTACCAACAACAGCAGTCGT+CGG + chr4_2:20373154-20373173 Msa0593820:CDS 45.0%
TCCAATTGCGAAGCAGCAAA+AGG + chr4_2:20373181-20373200 Msa0593820:CDS 45.0%
!! ACCTTTTGCTGCTTCGCAAT+TGG - chr4_2:20373185-20373204 None:intergenic 45.0%
GCTGATAGAAGCTGGTAATC+CGG + chr4_2:20373283-20373302 Msa0593820:CDS 45.0%
AATATCCCTTGCGCTTGAAC+CGG - chr4_2:20373305-20373324 None:intergenic 45.0%
AATCACCAGCTCTCACCAAT+TGG + chr4_2:20373335-20373354 Msa0593820:CDS 45.0%
CCTTTCCAAGGCTTCAATGA+GGG - chr4_2:20373540-20373559 None:intergenic 45.0%
ACCTTTCCAAGGCTTCAATG+AGG - chr4_2:20373541-20373560 None:intergenic 45.0%
TCAAAGAAATGACCCGGTAG+AGG - chr4_2:20373812-20373831 None:intergenic 45.0%
TAATCCATCATGCTTCCCTG+TGG + chr4_2:20373880-20373899 Msa0593820:CDS 45.0%
TGGGAAGAGGAACATCCATT+CGG - chr4_2:20373967-20373986 None:intergenic 45.0%
! GTAATTTGTCACGCTGGTTG+TGG - chr4_2:20374126-20374145 None:intergenic 45.0%
! ATGGAGGTAATTTGTCACGC+TGG - chr4_2:20374132-20374151 None:intergenic 45.0%
AGCGTGACAAATTACCTCCA+TGG + chr4_2:20374131-20374150 Msa0593820:CDS 45.0%
GTGACAAATTACCTCCATGG+AGG + chr4_2:20374134-20374153 Msa0593820:CDS 45.0%
AGAAACATGTTCCTCCATGG+AGG - chr4_2:20374148-20374167 None:intergenic 45.0%
GAATGTGAAGGGAGAGAAAC+CGG - chr4_2:20374176-20374195 None:intergenic 45.0%
CGTGAAGTTGCTGAATGTGA+AGG - chr4_2:20374188-20374207 None:intergenic 45.0%
! AAGCCGAGTGTTTGGTTCAA+AGG + chr4_2:20374215-20374234 Msa0593820:CDS 45.0%
! AGCCGAGTGTTTGGTTCAAA+GGG + chr4_2:20374216-20374235 Msa0593820:CDS 45.0%
TGCCCTTTGAACCAAACACT+CGG - chr4_2:20374221-20374240 None:intergenic 45.0%
ACCTGTGAGAGGATTTCAAG+TGG + chr4_2:20374240-20374259 Msa0593820:CDS 45.0%
TCCACTTGAAATCCTCTCAC+AGG - chr4_2:20374244-20374263 None:intergenic 45.0%
!!! TGTTTTTTAAATTTTTTAAT+GGG - chr4_2:20371779-20371798 None:intergenic 5.0%
!!! TTGTTTTTTAAATTTTTTAA+TGG - chr4_2:20371780-20371799 None:intergenic 5.0%
AGATGGATGCGTGCATACGT+AGG - chr4_2:20371833-20371852 None:intergenic 50.0%
!!! CTGGGGTAGTGGATTTTGTG+GGG + chr4_2:20372293-20372312 Msa0593820:intron 50.0%
!! TGGTGGTGGTGGTATGAGAA+TGG + chr4_2:20372794-20372813 Msa0593820:CDS 50.0%
!! TGGTGGTGGTATGAGAATGG+AGG + chr4_2:20372797-20372816 Msa0593820:CDS 50.0%
! TGGGTTGAACATGGACGCTT+CGG + chr4_2:20372977-20372996 Msa0593820:CDS 50.0%
ATGGACGCTTCGGTTCAACA+AGG + chr4_2:20372987-20373006 Msa0593820:CDS 50.0%
TGGACGCTTCGGTTCAACAA+GGG + chr4_2:20372988-20373007 Msa0593820:CDS 50.0%
CTGGATGCTTGCATCATCTC+CGG - chr4_2:20373222-20373241 None:intergenic 50.0%
ACTGCTGAGCTGATAGAAGC+TGG + chr4_2:20373275-20373294 Msa0593820:CDS 50.0%
GTAATCCGGTTCAAGCGCAA+GGG + chr4_2:20373297-20373316 Msa0593820:CDS 50.0%
GGTTCAAGCGCAAGGGATAT+TGG + chr4_2:20373304-20373323 Msa0593820:CDS 50.0%
GCTTACCAATTGGTGAGAGC+TGG - chr4_2:20373343-20373362 None:intergenic 50.0%
TCAAGCCCTCATTGAAGCCT+TGG + chr4_2:20373532-20373551 Msa0593820:CDS 50.0%
CCCTCATTGAAGCCTTGGAA+AGG + chr4_2:20373537-20373556 Msa0593820:CDS 50.0%
CTTGCCATCATGTCCTCTAC+CGG + chr4_2:20373796-20373815 Msa0593820:CDS 50.0%
TTGCCATCATGTCCTCTACC+GGG + chr4_2:20373797-20373816 Msa0593820:CDS 50.0%
AAAGCAGCTAAGGCCGAAAG+TGG + chr4_2:20373916-20373935 Msa0593820:CDS 50.0%
GCCGAAAGTGGTCGTAACTT+TGG + chr4_2:20373928-20373947 Msa0593820:CDS 50.0%
GGACAAAAACTGCGACCGAA+TGG + chr4_2:20373949-20373968 Msa0593820:CDS 50.0%
TGAACGACATTGGTGGGAAG+AGG - chr4_2:20373980-20373999 None:intergenic 50.0%
CAGCATAGCACAAGAGTGGA+AGG - chr4_2:20374274-20374293 None:intergenic 50.0%
CTTCCACTCTTGTGCTATGC+TGG + chr4_2:20374272-20374291 Msa0593820:CDS 50.0%
TGTGGAGCCCACTTCAGTTC+TGG + chr4_2:20372614-20372633 Msa0593820:CDS 55.0%
TGCTAGGAGACATCAGCAGC+AGG + chr4_2:20373085-20373104 Msa0593820:CDS 55.0%
CAACCGACGACTGCTGTTGT+TGG - chr4_2:20373160-20373179 None:intergenic 55.0%
GGTAATCCGGTTCAAGCGCA+AGG + chr4_2:20373296-20373315 Msa0593820:CDS 55.0%
AAGCGCAAGGGATATTGGCG+CGG + chr4_2:20373309-20373328 Msa0593820:CDS 55.0%
TGACCCGGTAGAGGACATGA+TGG - chr4_2:20373803-20373822 None:intergenic 55.0%
! AGAGCCACAGGGAAGCATGA+TGG - chr4_2:20373887-20373906 None:intergenic 55.0%
GGAAGCTCAAGCCGAGTGTT+TGG + chr4_2:20374207-20374226 Msa0593820:CDS 55.0%
!! GTTCAAAGGGCACCTGTGAG+AGG + chr4_2:20374229-20374248 Msa0593820:CDS 55.0%
CTGCCAGCATAGCACAAGAG+TGG - chr4_2:20374278-20374297 None:intergenic 55.0%
CTCTTGTGCTATGCTGGCAG+AGG + chr4_2:20374278-20374297 Msa0593820:CDS 55.0%
! CACGCTGGTTGTGGTGACCG+AGG - chr4_2:20374117-20374136 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 20371558 20374781 20371558 ID=Msa0593820;Name=Msa0593820
chr4_2 mRNA 20371558 20374781 20371558 ID=Msa0593820-mRNA-1;Parent=Msa0593820;Name=Msa0593820-mRNA-1;_AED=0.00;_eAED=0.00;_QI=451|1|1|1|0|0.5|2|445|625
chr4_2 exon 20371558 20372008 20371558 ID=Msa0593820-mRNA-1:exon:5778;Parent=Msa0593820-mRNA-1
chr4_2 exon 20372459 20374781 20372459 ID=Msa0593820-mRNA-1:exon:5779;Parent=Msa0593820-mRNA-1
chr4_2 five_prime_UTR 20371558 20372008 20371558 ID=Msa0593820-mRNA-1:five_prime_utr;Parent=Msa0593820-mRNA-1
chr4_2 CDS 20372459 20374336 20372459 ID=Msa0593820-mRNA-1:cds;Parent=Msa0593820-mRNA-1
chr4_2 three_prime_UTR 20374337 20374781 20374337 ID=Msa0593820-mRNA-1:three_prime_utr;Parent=Msa0593820-mRNA-1
Gene Sequence

>Msa0593820

ATGAAGGCTTTGCCCTTAACTTTTGAGGAATTTCAAGTGAAGGGAATGTTGGATAATTTCTCTTCAACTTCAAATTCAGTTTCAGATTCATTTTCACTGCTTTTGAATCAACCTCAGCAACAAAATTGGTCATTAGATAAAGAAGATTATTGCTATGTGGAGCCCACTTCAGTTCTGGATTCAAGAAGAAGCCCTAGTTCTCCATTTTCATCTTCAACATTGTCATCTTCTCATGGTAGTGGTAGTAATGGTAATGGTTCAAATAGCAATACTTCAATGGTAGTTTTTGAGAACCTTGCTCATCTTTCAAATGATGAGAATAGTGAAGAAAAATGTGGTGGTGGTGGTATGAGAATGGAGGATTGGGAAGGTCAAGATCAGTCACTTTTGAGGTTAATTATGGGAGATGTTGAAGACCCTTATGCTGGATTGAACAAAATCTTGCAAAGTGGTGGTTGTGGTTCTCAAAATGTTGATTTTCATGGTGGTTTTGGTGTTTTGGATCATCAACAACAACATGGGTTGAACATGGACGCTTCGGTTCAACAAGGGAATTACAATGTTTTTCCATTCATTCCTGAGAATTACAATGTGTTACCGCTTTTGGATTCTGGTCAAGAAGTGTTTGCTAGGAGACATCAGCAGCAGGAAACACAGCTTCCATTGTTTCCTCATCATCATGATCTGCAGCAGCATTACCAACAACAGCAGTCGTCGGTTGTTCCAATTGCGAAGCAGCAAAAGGTGAGTTCTAGTACTACCGGAGATGATGCAAGCATCCAGCTTCAGCAGAGTATATTTGATCAGCTATTCAAAACTGCTGAGCTGATAGAAGCTGGTAATCCGGTTCAAGCGCAAGGGATATTGGCGCGGCTCAATCACCAGCTCTCACCAATTGGTAAGCCTTTTCAAAGGGCTTCTTTCTACATGAAGGAAGCCTTACAGTTAATGCTTCATTCAAACGGTAACAATCTCACGGCTTTTTCGCCGATTAGTTTCATTTTCAAAATTGGAGCTTATAAGTCTTTTTCAGAAATATCACCCGTTCTTCAGTTTGCCAACTTTACTTGTAATCAAGCCCTCATTGAAGCCTTGGAAAGGTTCGATCGAATTCATGTTATTGATTTCGATATCGGGTTTGGAGTACAATGGTCGTCTTTTATGCAAGAGATTGCACTAAGAAGTAACGGTAAGCCTTCACTTAAGATTACTGCTGTTGTATCGCCCTCATCTTGCAACGAGATTGAACTCAATTTCACTCAAGAAAATTTGAGTCAGTATGCAAAAGACCTCAACATGTCGTTTAAGTTCAACGTTTTGAACATTGAATCGTTGAACTTGCCATCATGTCCTCTACCGGGTCATTTCTTTGATAGTAATGAAGCCATTGGTGTGAACTTTCCTGTTTCCAGTTTCATAAGTAATCCATCATGCTTCCCTGTGGCTCTTCATTTTTTAAAGCAGCTAAGGCCGAAAGTGGTCGTAACTTTGGACAAAAACTGCGACCGAATGGATGTTCCTCTTCCCACCAATGTCGTTCATGTTCTTCAATGTTACTCAGCTTTACTTGAATCACTTGATGCTGTGAACGTGAACCTCGATGTACTCCTAAAGATTGAAAGGCATTACATCCAACCAACCATCAACAAAATCATCCTCGGTCACCACAACCAGCGTGACAAATTACCTCCATGGAGGAACATGTTTCTTCAGTCCGGTTTCTCTCCCTTCACATTCAGCAACTTCACGGAAGCTCAAGCCGAGTGTTTGGTTCAAAGGGCACCTGTGAGAGGATTTCAAGTGGAAAGAAAGCCTTCCACTCTTGTGCTATGCTGGCAGAGGAAAGAACTCATCTCAATTTCAACTTGGAGATGTTGA
Protein sequence

>Msa0593820

MKALPLTFEEFQVKGMLDNFSSTSNSVSDSFSLLLNQPQQQNWSLDKEDYCYVEPTSVLDSRRSPSSPFSSSTLSSSHGSGSNGNGSNSNTSMVVFENLAHLSNDENSEEKCGGGGMRMEDWEGQDQSLLRLIMGDVEDPYAGLNKILQSGGCGSQNVDFHGGFGVLDHQQQHGLNMDASVQQGNYNVFPFIPENYNVLPLLDSGQEVFARRHQQQETQLPLFPHHHDLQQHYQQQQSSVVPIAKQQKVSSSTTGDDASIQLQQSIFDQLFKTAELIEAGNPVQAQGILARLNHQLSPIGKPFQRASFYMKEALQLMLHSNGNNLTAFSPISFIFKIGAYKSFSEISPVLQFANFTCNQALIEALERFDRIHVIDFDIGFGVQWSSFMQEIALRSNGKPSLKITAVVSPSSCNEIELNFTQENLSQYAKDLNMSFKFNVLNIESLNLPSCPLPGHFFDSNEAIGVNFPVSSFISNPSCFPVALHFLKQLRPKVVVTLDKNCDRMDVPLPTNVVHVLQCYSALLESLDAVNVNLDVLLKIERHYIQPTINKIILGHHNQRDKLPPWRNMFLQSGFSPFTFSNFTEAQAECLVQRAPVRGFQVERKPSTLVLCWQRKELISISTWRC*