AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594420 MtrunA17_Chr1g0163701 87.778 180 20 1 99 278 25 202 1.63e-92 304
Msa0594420 MtrunA17_Chr8g0389491 82.581 155 23 2 1 155 17 167 1.46e-72 240
Msa0594420 MtrunA17_Chr3g0140651 85.714 126 18 0 513 638 1573 1698 1.34e-57 219
Msa0594420 MtrunA17_Chr3g0115061 83.193 119 19 1 884 1001 124 242 1.03e-56 204
Msa0594420 MtrunA17_Chr3g0115061 91.139 79 6 1 998 1075 377 455 9.11e-41 157
Msa0594420 MtrunA17_Chr2g0305141 60.622 193 45 3 304 465 829 1021 1.58e-55 211
Msa0594420 MtrunA17_Chr7g0215371 91.667 84 7 0 656 739 14 97 2.01e-44 155
Msa0594420 MtrunA17_Chr1g0176351 70.930 86 23 1 50 135 2 85 1.14e-33 129
Msa0594420 MtrunA17_Chr2g0331241 78.409 88 19 0 155 242 63 150 1.11e-32 134
Msa0594420 MtrunA17_Chr2g0327751 77.273 66 14 1 1065 1130 423 487 1.19e-24 111
Msa0594420 MtrunA17_Chr2g0327751 85.000 40 6 0 848 887 346 385 2.15e-15 81.6
Msa0594420 MtrunA17_Chr1g0156121 81.967 61 11 0 656 716 119 179 2.25e-24 110
Msa0594420 MtrunA17_Chr2g0328251 61.176 85 28 3 50 131 2 84 1.97e-22 103
Msa0594420 MtrunA17_Chr5g0433421 46.154 130 52 6 752 869 238 361 6.83e-19 93.2
Msa0594420 MtrunA17_Chr5g0433361 74.074 54 12 1 752 803 281 334 1.04e-17 89.0
Msa0594420 MtrunA17_Chr5g0433511 37.931 174 78 10 752 912 255 411 1.51e-17 88.6
Msa0594420 MtrunA17_Chr4g0024031 50.000 122 55 4 50 168 2 120 3.14e-17 87.0
Msa0594420 MtrunA17_Chr5g0433471 76.596 47 11 0 757 803 249 295 1.86e-16 85.1
Msa0594420 MtrunA17_Chr2g0328241 53.684 95 39 3 51 142 3 95 7.73e-16 82.8
Msa0594420 MtrunA17_Chr5g0433351 68.519 54 15 1 752 803 245 298 2.08e-15 80.5
Msa0594420 MtrunA17_Chr5g0433371 45.745 94 34 3 712 803 210 288 1.60e-13 75.5
Msa0594420 MtrunA17_Chr4g0030281 63.514 74 21 1 448 515 257 330 1.06e-11 69.3
Msa0594420 MtrunA17_Chr3g0109541 30.097 206 119 9 750 942 241 434 2.68e-11 68.2
Msa0594420 MtrunA17_Chr5g0433321 56.140 57 23 2 749 803 234 290 9.75e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594420 AT2G17900.1 56.897 116 49 1 884 998 133 248 2.84e-37 147
Msa0594420 AT2G17900.1 69.444 72 22 0 1004 1075 393 464 8.76e-28 119
Msa0594420 AT4G32820.1 54.762 126 55 1 513 638 1516 1639 7.71e-34 142
Msa0594420 AT4G32820.2 54.762 126 55 1 513 638 1525 1648 8.21e-34 142
Msa0594420 AT4G32820.3 54.762 126 55 1 513 638 1516 1639 8.68e-34 142
Msa0594420 AT1G19870.2 52.500 80 37 1 661 740 32 110 9.89e-17 85.9
Msa0594420 AT1G19870.1 52.500 80 37 1 661 740 215 293 1.26e-16 85.9

Find 236 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGCAAGCTGCATATTTC+TGG 0.146566 4_2:-22220423 None:intergenic
GAGAATGTTTGCTTTATTTA+TGG 0.171491 4_2:+22220383 Msa0594420:CDS
GAGAACCAATTCCGGTTATT+AGG 0.172098 4_2:+22218813 Msa0594420:CDS
TGTGAAGGCTGCCACAAATT+TGG 0.205381 4_2:+22221937 Msa0594420:CDS
ATTGCATTGTTGGAGATAAA+TGG 0.208228 4_2:+22220501 Msa0594420:CDS
ATGCTAATGTCAAACTTATA+TGG 0.215459 4_2:+22221069 Msa0594420:CDS
TGCAACTGTCTTTGTCTTCC+CGG 0.227998 4_2:-22221105 None:intergenic
GGTTGCACACTCGCCTGTTT+TGG 0.231955 4_2:+22219771 Msa0594420:CDS
ATCTCTTTAATATTGATTTC+AGG 0.234425 4_2:-22221500 None:intergenic
TATGCTGCTTCAATAGTTAA+TGG 0.245321 4_2:+22220624 Msa0594420:CDS
AATGAAAGGGGAGAAATTTA+AGG 0.249586 4_2:+22220926 Msa0594420:CDS
TATGCCGGAGATTGTGATTT+CGG 0.253606 4_2:+22219708 Msa0594420:CDS
ATGCTTCCTGCGCACATCAT+AGG 0.266732 4_2:+22221990 Msa0594420:CDS
ATGCCGGAGATTGTGATTTC+GGG 0.274770 4_2:+22219709 Msa0594420:CDS
ATATGCAGCTTGCATGAAAT+TGG 0.275686 4_2:+22220429 Msa0594420:CDS
AACCATGGATCTGGAGGTTT+TGG 0.285618 4_2:-22220180 None:intergenic
GAATTGTAAAGTAGAAATTA+TGG 0.311895 4_2:-22222158 None:intergenic
CAGCAACAGCTTGAAGTTGT+TGG 0.318313 4_2:-22220047 None:intergenic
AGATCGAAAATTTGAATTAT+CGG 0.318465 4_2:-22221321 None:intergenic
TTCGATTGAGGATTTGTTCC+AGG 0.320753 4_2:-22219799 None:intergenic
AATTGCACTTCATGTTAATC+AGG 0.336059 4_2:+22219510 Msa0594420:CDS
ACTAATTCATCTACATAAAC+TGG 0.340530 4_2:-22219439 None:intergenic
TCTTGCTCTTCACAAACATT+AGG 0.340971 4_2:-22222058 None:intergenic
TGCTGGTTGCAGTCAATTGC+AGG 0.341092 4_2:+22219351 Msa0594420:CDS
TTGTGATTCGGAGCTGAAAA+TGG 0.341881 4_2:-22220870 None:intergenic
ATTTCTAAACATGTTGGGTT+TGG 0.343214 4_2:+22219862 Msa0594420:CDS
ATATGCCAATGAATCTGATT+TGG 0.343250 4_2:+22218880 Msa0594420:CDS
GAAATTTAAGGAAGATGATT+GGG 0.344363 4_2:+22220938 Msa0594420:CDS
TTTCGGTACGTAACAATAGT+AGG 0.349766 4_2:-22218851 None:intergenic
AAAGCAAACTTAGCATTCTA+AGG 0.350820 4_2:-22221208 None:intergenic
AGACATGCTTTCAGAATTGC+AGG 0.351995 4_2:+22219025 Msa0594420:CDS
CTGAATCGTAGATTTCAAAT+TGG 0.359013 4_2:-22218949 None:intergenic
AAGCATTAAGCATGCTAGTT+GGG 0.360383 4_2:-22221973 None:intergenic
ATTGTTTCTGTTGTTGTTGT+TGG 0.362999 4_2:-22218777 None:intergenic
CAAACCCTAATAACCGGAAT+TGG 0.366700 4_2:-22218818 None:intergenic
ATCATCCAGGCTTCAATTAG+AGG 0.366920 4_2:+22220714 Msa0594420:CDS
TGCTAATGTCAAACTTATAT+GGG 0.367336 4_2:+22221070 Msa0594420:CDS
ATGATTATCGCACCAGATTC+CGG 0.368081 4_2:-22220696 None:intergenic
AGAACCAATTCCGGTTATTA+GGG 0.369746 4_2:+22218814 Msa0594420:CDS
CTGGTCTAACAAAGCTTTCA+TGG 0.369804 4_2:-22218717 None:intergenic
AATCAGTAAAGCTAGAATAT+TGG 0.371643 4_2:+22221717 Msa0594420:CDS
AATGTCAATCCTCCGGAATC+TGG 0.373265 4_2:+22220684 Msa0594420:CDS
GGATACACTCTTTGATAGAT+TGG 0.375546 4_2:-22221773 None:intergenic
CAAGCTGCTGTGATTGATAA+TGG 0.378940 4_2:-22221615 None:intergenic
TGACATTAGGCTCAATAAAC+AGG 0.379269 4_2:-22220668 None:intergenic
TTGAAGTTGTTGGTCTTCCT+TGG 0.382764 4_2:-22220037 None:intergenic
TACTTTACAATTCCAACAAT+GGG 0.385747 4_2:+22222168 Msa0594420:CDS
GAGTTGAGACCTTTGGGAAC+AGG 0.386894 4_2:+22221575 Msa0594420:CDS
ATATGGGGACCAACAGGAAC+CGG 0.391798 4_2:+22221086 Msa0594420:CDS
GTTGGAGATAAATGGAAAAC+TGG 0.391848 4_2:+22220509 Msa0594420:CDS
GGTGATCTCCTTCACGATAT+AGG 0.393326 4_2:-22220223 None:intergenic
TGATCTATTTCAGAGTTAGA+AGG 0.393336 4_2:-22221182 None:intergenic
CTAGAAATTACCGATGGCTT+TGG 0.394323 4_2:-22220120 None:intergenic
TTCGCGGTCACTTGGTTAGA+AGG 0.395082 4_2:+22220796 Msa0594420:CDS
CCAGCTGTTCACCCTTTGCT+TGG 0.395564 4_2:+22221794 Msa0594420:CDS
ACCGATGGCTTTGGCTTTGT+AGG 0.399512 4_2:-22220111 None:intergenic
TGACTTCTGTCATCGAGTTC+AGG 0.400277 4_2:-22221248 None:intergenic
CCCATTGTTGAATTGTGATT+CGG 0.401021 4_2:-22220882 None:intergenic
TATGCAGCTTGCATGAAATT+GGG 0.402684 4_2:+22220430 Msa0594420:CDS
TTCAGGAAGCAGATTTGAAC+TGG 0.405127 4_2:-22221231 None:intergenic
GCATGACCATATTGCATTGT+TGG 0.405312 4_2:+22220491 Msa0594420:CDS
ATTGGTTCTCCTCCTCTGTT+CGG 0.409429 4_2:-22218800 None:intergenic
CTTCAATTTCTAAACATGTT+GGG 0.413282 4_2:+22219857 Msa0594420:CDS
GTATTCCAAATCAGATTCAT+TGG 0.413673 4_2:-22218885 None:intergenic
GTTCCAGGACTTCCCAAAAC+AGG 0.414066 4_2:-22219784 None:intergenic
TTATGGAGCAGAGAAGCTTA+TGG 0.416741 4_2:+22220400 Msa0594420:CDS
AAAATTACAGAATCGGTTGC+TGG 0.419127 4_2:+22219334 Msa0594420:CDS
TGACAGTGAGTTGAGACCTT+TGG 0.421001 4_2:+22221568 Msa0594420:CDS
GACCACCCGGGTCCCATTGT+TGG 0.423019 4_2:-22222180 None:intergenic
CAAGATCATGTCTGCTCACT+GGG 0.423727 4_2:+22222099 Msa0594420:CDS
GGAAAACTTGAATGGTATCT+TGG 0.424746 4_2:+22221836 Msa0594420:CDS
TGACATCACAGAGGAGCATT+TGG 0.425504 4_2:+22221430 Msa0594420:CDS
GGTGGTCTCTTGTTCATTGA+CGG 0.427125 4_2:+22222196 Msa0594420:CDS
CACAATTCAACAATGGGATT+AGG 0.428502 4_2:+22220888 Msa0594420:CDS
GACAGTGAGTTGAGACCTTT+GGG 0.429742 4_2:+22221569 Msa0594420:CDS
CAATTAGAGGATATTTGATT+CGG 0.430573 4_2:+22220727 Msa0594420:CDS
GTGACTGAATGTTCTTCAAC+CGG 0.434174 4_2:-22219592 None:intergenic
TTTGGTGTTATATGCACAAA+TGG 0.438018 4_2:+22221448 Msa0594420:CDS
AGAAATTTAAGGAAGATGAT+TGG 0.440291 4_2:+22220937 Msa0594420:CDS
TATCCAACTCACTCTAGGTT+CGG 0.440411 4_2:-22219566 None:intergenic
GATTCCGGAGGATTGACATT+AGG 0.440683 4_2:-22220681 None:intergenic
TTATTACAAACCCTAATAAC+CGG 0.449449 4_2:-22218824 None:intergenic
GATGTTGAAAACAAAGTTCT+AGG 0.450761 4_2:+22219376 Msa0594420:CDS
GTCAGATATGTGAGCTACTA+AGG 0.452229 4_2:-22221411 None:intergenic
CCAAGCAAAGGGTGAACAGC+TGG 0.455085 4_2:-22221794 None:intergenic
CCATGGTTCTCGACGACGAC+GGG 0.455528 4_2:+22220195 Msa0594420:CDS
TAGCTGTCCACGCCACCATC+TGG 0.455607 4_2:-22218736 None:intergenic
GCAAGATCATGTCTGCTCAC+TGG 0.455909 4_2:+22222098 Msa0594420:CDS
AAACTTATATGGGGACCAAC+AGG 0.456440 4_2:+22221080 Msa0594420:CDS
TTCTCCTCAATACTAACAAC+AGG 0.457927 4_2:-22219619 None:intergenic
CAGAGGAGGAGAACCAATTC+CGG 0.457954 4_2:+22218805 Msa0594420:CDS
TTGGTTAGAAGGCATGTTGT+TGG 0.458528 4_2:+22220807 Msa0594420:CDS
TAGATTGAATTCCAAATTTG+TGG 0.459462 4_2:-22221948 None:intergenic
GTTAATCAGGCACGTCTTGA+GGG 0.459816 4_2:+22219523 Msa0594420:CDS
AGCTGTTGTTCGCGGTCACT+TGG 0.459866 4_2:+22220788 Msa0594420:CDS
GTCAATATCGCGAGAATGAA+AGG 0.460190 4_2:+22220912 Msa0594420:CDS
ACCGTTATCGTGTATAATCC+AGG 0.461084 4_2:-22219929 None:intergenic
TCTTCAATTTCTAAACATGT+TGG 0.462683 4_2:+22219856 Msa0594420:CDS
TATGGCTAAAATTACAGAAT+CGG 0.463234 4_2:+22219327 Msa0594420:CDS
TTTGTGGCAGCCTTCACAAA+TGG 0.466152 4_2:-22221932 None:intergenic
AAATTGCATCATTCTTCTGA+TGG 0.470831 4_2:-22219831 None:intergenic
TTCTGAAAGCATGTCTCAAA+AGG 0.471750 4_2:-22219018 None:intergenic
TAAATCATTGACCAAGGCTC+TGG 0.472219 4_2:+22221877 Msa0594420:CDS
TATTGCAGAATTGTGTGTTC+CGG 0.475079 4_2:+22220149 Msa0594420:CDS
TAGTTGTCCCTAGCAGTGCT+TGG 0.478382 4_2:-22221374 None:intergenic
AACAAAGATTACTCCTTGAC+CGG 0.478687 4_2:+22218995 Msa0594420:CDS
CGAACCTGTTGTTAGTATTG+AGG 0.481267 4_2:+22219615 Msa0594420:CDS
TAATCTTTGTTGTAACAAAA+CGG 0.481557 4_2:-22218983 None:intergenic
GTCGTCGAGAACCATGGATC+TGG 0.481594 4_2:-22220189 None:intergenic
CTGATTGTACTCCACTGGCC+TGG 0.483075 4_2:-22219749 None:intergenic
TTATCTCCAACAATGCAATA+TGG 0.485008 4_2:-22220497 None:intergenic
TGCTTCCTGCGCACATCATA+GGG 0.485701 4_2:+22221991 Msa0594420:CDS
ATGCGCATTACATGCAAACT+TGG 0.485744 4_2:-22221537 None:intergenic
CATCTGAGAGTATCATGTAT+AGG 0.489877 4_2:+22220463 Msa0594420:CDS
CCGAATCACAATTCAACAAT+GGG 0.491019 4_2:+22220882 Msa0594420:CDS
TGTTAATCAGGCACGTCTTG+AGG 0.492841 4_2:+22219522 Msa0594420:CDS
CAGGCCAGTGGAGTACAATC+AGG 0.493111 4_2:+22219750 Msa0594420:CDS
TAGAGTGAGTTGGATAAAAC+CGG 0.495923 4_2:+22219573 Msa0594420:CDS
AATTCTTACGACGTTGAAAG+AGG 0.499264 4_2:+22219117 Msa0594420:CDS
ATCTGGTGCGATAATCATCC+AGG 0.501379 4_2:+22220701 Msa0594420:CDS
TTGTGAAGAAAGCTTGGTAA+TGG 0.503125 4_2:+22219201 Msa0594420:CDS
CCAAGGCTCTGGATATACTA+AGG 0.503243 4_2:+22221888 Msa0594420:CDS
ACGAATTACGTCTTTAACAA+AGG 0.503489 4_2:-22220979 None:intergenic
TAAATATTACACAAAGTTGA+AGG 0.503711 4_2:+22218916 Msa0594420:CDS
TCTCAACTCACTGTCACAAA+TGG 0.504130 4_2:-22221561 None:intergenic
TGTAGATGAATTAGTAAAGG+TGG 0.505027 4_2:+22219447 Msa0594420:CDS
TAACTCATGTTCGCGTAAGA+TGG 0.506658 4_2:+22219048 Msa0594420:CDS
GAAGGTAGCTGCCGAAGCTA+TGG 0.507018 4_2:+22219309 Msa0594420:CDS
CTACTTTACAATTCCAACAA+TGG 0.508550 4_2:+22222167 Msa0594420:CDS
TTTGAGGGTCAATTGAATGA+TGG 0.510847 4_2:+22219964 Msa0594420:CDS
CTCAATACTAACAACAGGTT+CGG 0.511030 4_2:-22219614 None:intergenic
ATATCATTCCAAGCACTGCT+AGG 0.511042 4_2:+22221366 Msa0594420:CDS
TTTGTCTTCCCGGTTCCTGT+TGG 0.511536 4_2:-22221095 None:intergenic
GAAAGTTCAGCTGCATCTGT+TGG 0.512071 4_2:+22220069 Msa0594420:CDS
GGTGTTGAACAAAAGTTTGA+GGG 0.515338 4_2:+22219949 Msa0594420:CDS
AATATCCTCTAATTGAAGCC+TGG 0.517071 4_2:-22220719 None:intergenic
ATGTTTGTGAAGAGCAAGAA+AGG 0.517683 4_2:+22222062 Msa0594420:CDS
GAAGCATTAAGCATGCTAGT+TGG 0.517929 4_2:-22221974 None:intergenic
ATGCTTGTGGAAAACTTGAA+TGG 0.524944 4_2:+22221828 Msa0594420:CDS
CGGTGTTGAACAAAAGTTTG+AGG 0.525583 4_2:+22219948 Msa0594420:CDS
GGAGATAAAGTTCAAGAAGT+GGG 0.526531 4_2:+22219247 Msa0594420:CDS
CAGCTTGCCCGATTCAATGT+TGG 0.526935 4_2:+22221631 Msa0594420:CDS
TGAGTTGTACTAATCCCATT+GGG 0.528046 4_2:-22221271 None:intergenic
CCTTAGTATATCCAGAGCCT+TGG 0.528936 4_2:-22221888 None:intergenic
GGAGTCAGTATCAGAAACAT+TGG 0.529789 4_2:+22219468 Msa0594420:CDS
GGCTTGCAATATTATGCTTG+TGG 0.530814 4_2:+22221815 Msa0594420:CDS
GAACCCGAAATCACAATCTC+CGG 0.531441 4_2:-22219712 None:intergenic
AAGTTGCAAGCTGTTGTTCG+CGG 0.532219 4_2:+22220780 Msa0594420:CDS
AATTTAAATGATGCAAAAGA+AGG 0.532407 4_2:+22219655 Msa0594420:CDS
TGAGCCTAATGTCAATCCTC+CGG 0.532718 4_2:+22220677 Msa0594420:CDS
AACAGATGTCGAAAACGCTT+CGG 0.533722 4_2:-22219161 None:intergenic
GCGACAATTGAATCAAAAGA+TGG 0.542926 4_2:+22219226 Msa0594420:CDS
TGTTGGGTTTGGACATGTGC+AGG 0.543371 4_2:+22219873 Msa0594420:CDS
ATGTCTCAAAAGGCCGGTCA+AGG 0.544729 4_2:-22219008 None:intergenic
TTCGGTACGTAACAATAGTA+GGG 0.544923 4_2:-22218850 None:intergenic
CTTTACAACATGTACACAAA+GGG 0.545306 4_2:+22221681 Msa0594420:CDS
TCAATATCGCGAGAATGAAA+GGG 0.547217 4_2:+22220913 Msa0594420:CDS
GTTCATATGCTAATAAATCC+AGG 0.549627 4_2:+22219731 Msa0594420:CDS
TAGGGACAACTACAAGTTAG+TGG 0.549677 4_2:+22221385 Msa0594420:CDS
TCACTGGGAGCTCAAGACTA+AGG 0.550401 4_2:+22222114 Msa0594420:CDS
GAAAGCTTTGTTAGACCAGA+TGG 0.551407 4_2:+22218721 Msa0594420:CDS
TAATATTGCAAGCCAAGCAA+AGG 0.552972 4_2:-22221806 None:intergenic
CACTCTTTGATAGATTGGTA+TGG 0.556224 4_2:-22221768 None:intergenic
TTGAGTTGTACTAATCCCAT+TGG 0.556787 4_2:-22221272 None:intergenic
TGGAGATAAAGTTCAAGAAG+TGG 0.557705 4_2:+22219246 Msa0594420:CDS
ACAATTCCAACAATGGGACC+CGG 0.559676 4_2:+22222174 Msa0594420:CDS
CACCAAAACCTCCAGATCCA+TGG 0.560015 4_2:+22220178 Msa0594420:CDS
GATGGTGGCGTGGACAGCTA+AGG 0.560948 4_2:+22218739 Msa0594420:CDS
TTATGTAGATGAATTAGTAA+AGG 0.561602 4_2:+22219444 Msa0594420:CDS
GAAAGCATGTCTCAAAAGGC+CGG 0.562709 4_2:-22219014 None:intergenic
AGCATTAAGCATGCTAGTTG+GGG 0.562946 4_2:-22221972 None:intergenic
TAAATACTTTCTGATAGCCA+CGG 0.564270 4_2:-22220283 None:intergenic
CTCTAATGAGCATCACATGA+AGG 0.565141 4_2:+22219291 Msa0594420:CDS
AAGTTCTTGTGAAGAAAGCT+TGG 0.566019 4_2:+22219195 Msa0594420:CDS
ATGATACTCTCAGATGTACC+AGG 0.566151 4_2:-22220456 None:intergenic
AAATTTAAGGAAGATGATTG+GGG 0.568109 4_2:+22220939 Msa0594420:CDS
GCAACCTGATTGTACTCCAC+TGG 0.571215 4_2:-22219754 None:intergenic
CAACTACTATTTGAGAGTGT+AGG 0.573815 4_2:-22220579 None:intergenic
AAATACTTTCTGATAGCCAC+GGG 0.574438 4_2:-22220282 None:intergenic
CCCGTCGTCGTCGAGAACCA+TGG 0.576306 4_2:-22220195 None:intergenic
GAGTCAGTATCAGAAACATT+GGG 0.577261 4_2:+22219469 Msa0594420:CDS
CAATTCCAACAATGGGACCC+GGG 0.580754 4_2:+22222175 Msa0594420:CDS
ACCTACAAAGCCAAAGCCAT+CGG 0.580832 4_2:+22220110 Msa0594420:CDS
CCCTGGATTATACACGATAA+CGG 0.582079 4_2:+22219928 Msa0594420:CDS
TATCATTCCAAGCACTGCTA+GGG 0.582091 4_2:+22221367 Msa0594420:CDS
ACATTGTCAGAACTACTCAC+TGG 0.582734 4_2:-22220354 None:intergenic
TTAGAAGGTTCAAAACAAGC+AGG 0.583771 4_2:-22221167 None:intergenic
TCCGAATCACAATTCAACAA+TGG 0.584304 4_2:+22220881 Msa0594420:CDS
TCGCCGAACCTAGAGTGAGT+TGG 0.586741 4_2:+22219563 Msa0594420:CDS
AATATTGCAAGCCAAGCAAA+GGG 0.586895 4_2:-22221805 None:intergenic
GATGACAGAAGTCACCCAAT+GGG 0.587705 4_2:+22221257 Msa0594420:CDS
TTGACGGAGTCAACAAGCAT+GGG 0.590532 4_2:+22222212 Msa0594420:CDS
TCAATGAACAAGAGACCACC+CGG 0.591252 4_2:-22222193 None:intergenic
TTGCATGAAATTGGGGACCC+TGG 0.595617 4_2:+22220438 Msa0594420:CDS
GTGTCGAGTAGTTACTATGC+CGG 0.596592 4_2:+22219693 Msa0594420:CDS
ATTGACGGAGTCAACAAGCA+TGG 0.597028 4_2:+22222211 Msa0594420:CDS
GAAGACAAAGACAGTTGCAT+GGG 0.598974 4_2:+22221109 Msa0594420:CDS
CGATGACAGAAGTCACCCAA+TGG 0.601219 4_2:+22221256 Msa0594420:CDS
ATGCAGCTTGCATGAAATTG+GGG 0.605861 4_2:+22220431 Msa0594420:CDS
GCTGCTTCAATAGTTAATGG+TGG 0.609962 4_2:+22220627 Msa0594420:CDS
TAAGGAATTAGAATCACAGT+TGG 0.612419 4_2:+22222132 Msa0594420:CDS
TATGGGGACCAACAGGAACC+GGG 0.615207 4_2:+22221087 Msa0594420:CDS
TGATGCCCTATGATGTGCGC+AGG 0.615349 4_2:-22221996 None:intergenic
CATTGAAGATCTTGTACCCG+TGG 0.615407 4_2:+22220266 Msa0594420:CDS
CCGTTATCGTGTATAATCCA+GGG 0.615444 4_2:-22219928 None:intergenic
AGAAACAATCCGAACAGAGG+AGG 0.617511 4_2:+22218791 Msa0594420:CDS
GCTGGTTGCAGTCAATTGCA+GGG 0.617729 4_2:+22219352 Msa0594420:CDS
TGCTAATAAATCCAGGCCAG+TGG 0.619546 4_2:+22219738 Msa0594420:CDS
GAGTAGTTCTGACAATGTGT+TGG 0.625077 4_2:+22220359 Msa0594420:CDS
TACAAAGACTCCATTTGTGA+AGG 0.626312 4_2:+22221922 Msa0594420:CDS
GGAAGACAAAGACAGTTGCA+TGG 0.626328 4_2:+22221108 Msa0594420:CDS
TGATATAATCCTGTTCCCAA+AGG 0.626743 4_2:-22221584 None:intergenic
TTAATCAGGCACGTCTTGAG+GGG 0.632115 4_2:+22219524 Msa0594420:CDS
GTCGAGAACCATGGATCTGG+AGG 0.634219 4_2:-22220186 None:intergenic
GCTTTACAACATGTACACAA+AGG 0.634688 4_2:+22221680 Msa0594420:CDS
GATATACTAAGGATTACTCA+TGG 0.637324 4_2:+22221899 Msa0594420:CDS
AACAGAAACAATCCGAACAG+AGG 0.642385 4_2:+22218788 Msa0594420:CDS
AAAAGAAGTTCGATATACCA+AGG 0.642415 4_2:+22220020 Msa0594420:CDS
CAATATCGCGAGAATGAAAG+GGG 0.643003 4_2:+22220914 Msa0594420:CDS
GCTAATGTCAAACTTATATG+GGG 0.644828 4_2:+22221071 Msa0594420:CDS
GTTGAAAACAAAGTTCTAGG+AGG 0.655970 4_2:+22219379 Msa0594420:CDS
TCCATGGTTCTCGACGACGA+CGG 0.660617 4_2:+22220194 Msa0594420:CDS
GGGGAAATCCTATATCGTGA+AGG 0.666362 4_2:+22220215 Msa0594420:CDS
AGCTGTTAAATCATTGACCA+AGG 0.667636 4_2:+22221871 Msa0594420:CDS
TGTTAGACCAGATGGTGGCG+TGG 0.671286 4_2:+22218729 Msa0594420:CDS
TGAATGGTATCTTGGAGACA+CGG 0.675211 4_2:+22221844 Msa0594420:CDS
CATGGTTCTCGACGACGACG+GGG 0.682796 4_2:+22220196 Msa0594420:CDS
AGCTTTGTTAGACCAGATGG+TGG 0.683046 4_2:+22218724 Msa0594420:CDS
TGATACTCTCAGATGTACCA+GGG 0.687333 4_2:-22220455 None:intergenic
ACATGTACACAAAGGGACTG+AGG 0.688671 4_2:+22221688 Msa0594420:CDS
ATTATCGCACCAGATTCCGG+AGG 0.691340 4_2:-22220693 None:intergenic
TCACTGCTAGAAATTACCGA+TGG 0.693196 4_2:-22220126 None:intergenic
TTCCAACAATGGGACCCGGG+TGG 0.731563 4_2:+22222178 Msa0594420:CDS
ATGGTATCTTGGAGACACGG+TGG 0.756768 4_2:+22221847 Msa0594420:CDS
CAATGAACAAGAGACCACCC+GGG 0.774541 4_2:-22222192 None:intergenic
TCACATATCTGACATCACAG+AGG 0.795706 4_2:+22221421 Msa0594420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATATTACACAAAGTTGA+AGG + chr4_2:22218916-22218935 Msa0594420:CDS 20.0%
!!! TAATCTTTGTTGTAACAAAA+CGG - chr4_2:22218986-22219005 None:intergenic 20.0%
!! TTATGTAGATGAATTAGTAA+AGG + chr4_2:22219444-22219463 Msa0594420:CDS 20.0%
!! AATTTAAATGATGCAAAAGA+AGG + chr4_2:22219655-22219674 Msa0594420:CDS 20.0%
!! AGATCGAAAATTTGAATTAT+CGG - chr4_2:22221324-22221343 None:intergenic 20.0%
!!! TTTTACATGTAATACTACAA+TGG + chr4_2:22221477-22221496 Msa0594420:CDS 20.0%
!!! ATCTCTTTAATATTGATTTC+AGG - chr4_2:22221503-22221522 None:intergenic 20.0%
!! GAATTGTAAAGTAGAAATTA+TGG - chr4_2:22222161-22222180 None:intergenic 20.0%
! TTATTACAAACCCTAATAAC+CGG - chr4_2:22218827-22218846 None:intergenic 25.0%
!!! CATTGGCATATTTTGATTTT+CGG - chr4_2:22218871-22218890 None:intergenic 25.0%
! TATGGCTAAAATTACAGAAT+CGG + chr4_2:22219327-22219346 Msa0594420:CDS 25.0%
! ACTAATTCATCTACATAAAC+TGG - chr4_2:22219442-22219461 None:intergenic 25.0%
! TCTTCAATTTCTAAACATGT+TGG + chr4_2:22219856-22219875 Msa0594420:CDS 25.0%
! CTTCAATTTCTAAACATGTT+GGG + chr4_2:22219857-22219876 Msa0594420:CDS 25.0%
!!! GAGAATGTTTGCTTTATTTA+TGG + chr4_2:22220383-22220402 Msa0594420:CDS 25.0%
! CAATTAGAGGATATTTGATT+CGG + chr4_2:22220727-22220746 Msa0594420:CDS 25.0%
! AGAAATTTAAGGAAGATGAT+TGG + chr4_2:22220937-22220956 Msa0594420:CDS 25.0%
! GAAATTTAAGGAAGATGATT+GGG + chr4_2:22220938-22220957 Msa0594420:CDS 25.0%
! AAATTTAAGGAAGATGATTG+GGG + chr4_2:22220939-22220958 Msa0594420:CDS 25.0%
! ATGCTAATGTCAAACTTATA+TGG + chr4_2:22221069-22221088 Msa0594420:CDS 25.0%
! TGCTAATGTCAAACTTATAT+GGG + chr4_2:22221070-22221089 Msa0594420:CDS 25.0%
! TTCAAAAACCAACATTGAAT+CGG - chr4_2:22221642-22221661 None:intergenic 25.0%
! AATCAGTAAAGCTAGAATAT+TGG + chr4_2:22221717-22221736 Msa0594420:CDS 25.0%
! TAGATTGAATTCCAAATTTG+TGG - chr4_2:22221951-22221970 None:intergenic 25.0%
! TACTTTACAATTCCAACAAT+GGG + chr4_2:22222168-22222187 Msa0594420:CDS 25.0%
!! ATTGTTTCTGTTGTTGTTGT+TGG - chr4_2:22218780-22218799 None:intergenic 30.0%
ATATGCCAATGAATCTGATT+TGG + chr4_2:22218880-22218899 Msa0594420:CDS 30.0%
GTATTCCAAATCAGATTCAT+TGG - chr4_2:22218888-22218907 None:intergenic 30.0%
CTGAATCGTAGATTTCAAAT+TGG - chr4_2:22218952-22218971 None:intergenic 30.0%
!! TGTAATTTTAGCCATAGCTT+CGG - chr4_2:22219323-22219342 None:intergenic 30.0%
GATGTTGAAAACAAAGTTCT+AGG + chr4_2:22219376-22219395 Msa0594420:CDS 30.0%
!! AGAAATTGAATGCTTTTTGG+TGG - chr4_2:22219416-22219435 None:intergenic 30.0%
! CACAGAAATTGAATGCTTTT+TGG - chr4_2:22219419-22219438 None:intergenic 30.0%
TGTAGATGAATTAGTAAAGG+TGG + chr4_2:22219447-22219466 Msa0594420:CDS 30.0%
AATTGCACTTCATGTTAATC+AGG + chr4_2:22219510-22219529 Msa0594420:CDS 30.0%
GTTCATATGCTAATAAATCC+AGG + chr4_2:22219731-22219750 Msa0594420:CDS 30.0%
AAATTGCATCATTCTTCTGA+TGG - chr4_2:22219834-22219853 None:intergenic 30.0%
ATTTCTAAACATGTTGGGTT+TGG + chr4_2:22219862-22219881 Msa0594420:CDS 30.0%
!!! AAACTCACTTTTTAGCAATC+AGG + chr4_2:22219903-22219922 Msa0594420:CDS 30.0%
AAAAGAAGTTCGATATACCA+AGG + chr4_2:22220020-22220039 Msa0594420:CDS 30.0%
GCATAATGAAAAATCAGTAG+TGG - chr4_2:22220247-22220266 None:intergenic 30.0%
TAAATACTTTCTGATAGCCA+CGG - chr4_2:22220286-22220305 None:intergenic 30.0%
TTATCTCCAACAATGCAATA+TGG - chr4_2:22220500-22220519 None:intergenic 30.0%
ATTGCATTGTTGGAGATAAA+TGG + chr4_2:22220501-22220520 Msa0594420:CDS 30.0%
! TATGCTGCTTCAATAGTTAA+TGG + chr4_2:22220624-22220643 Msa0594420:CDS 30.0%
AATGAAAGGGGAGAAATTTA+AGG + chr4_2:22220926-22220945 Msa0594420:CDS 30.0%
ACGAATTACGTCTTTAACAA+AGG - chr4_2:22220982-22221001 None:intergenic 30.0%
GCTAATGTCAAACTTATATG+GGG + chr4_2:22221071-22221090 Msa0594420:CDS 30.0%
TGATCTATTTCAGAGTTAGA+AGG - chr4_2:22221185-22221204 None:intergenic 30.0%
AAAGCAAACTTAGCATTCTA+AGG - chr4_2:22221211-22221230 None:intergenic 30.0%
!! TTTGGTGTTATATGCACAAA+TGG + chr4_2:22221448-22221467 Msa0594420:CDS 30.0%
TCAAAAACCAACATTGAATC+GGG - chr4_2:22221641-22221660 None:intergenic 30.0%
!!! GATTCAATGTTGGTTTTTGA+AGG + chr4_2:22221641-22221660 Msa0594420:CDS 30.0%
CTTTACAACATGTACACAAA+GGG + chr4_2:22221681-22221700 Msa0594420:CDS 30.0%
GATATACTAAGGATTACTCA+TGG + chr4_2:22221899-22221918 Msa0594420:CDS 30.0%
TAAGGAATTAGAATCACAGT+TGG + chr4_2:22222132-22222151 Msa0594420:CDS 30.0%
CTACTTTACAATTCCAACAA+TGG + chr4_2:22222167-22222186 Msa0594420:CDS 30.0%
AGAACCAATTCCGGTTATTA+GGG + chr4_2:22218814-22218833 Msa0594420:CDS 35.0%
TTCGGTACGTAACAATAGTA+GGG - chr4_2:22218853-22218872 None:intergenic 35.0%
TTTCGGTACGTAACAATAGT+AGG - chr4_2:22218854-22218873 None:intergenic 35.0%
AACAAAGATTACTCCTTGAC+CGG + chr4_2:22218995-22219014 Msa0594420:CDS 35.0%
! TTCTGAAAGCATGTCTCAAA+AGG - chr4_2:22219021-22219040 None:intergenic 35.0%
AATTCTTACGACGTTGAAAG+AGG + chr4_2:22219117-22219136 Msa0594420:CDS 35.0%
AAGTTCTTGTGAAGAAAGCT+TGG + chr4_2:22219195-22219214 Msa0594420:CDS 35.0%
TTGTGAAGAAAGCTTGGTAA+TGG + chr4_2:22219201-22219220 Msa0594420:CDS 35.0%
GCGACAATTGAATCAAAAGA+TGG + chr4_2:22219226-22219245 Msa0594420:CDS 35.0%
TGGAGATAAAGTTCAAGAAG+TGG + chr4_2:22219246-22219265 Msa0594420:CDS 35.0%
GGAGATAAAGTTCAAGAAGT+GGG + chr4_2:22219247-22219266 Msa0594420:CDS 35.0%
AAAATTACAGAATCGGTTGC+TGG + chr4_2:22219334-22219353 Msa0594420:CDS 35.0%
!! GTTGAAAACAAAGTTCTAGG+AGG + chr4_2:22219379-22219398 Msa0594420:CDS 35.0%
GAGTCAGTATCAGAAACATT+GGG + chr4_2:22219469-22219488 Msa0594420:CDS 35.0%
! TAGAGTGAGTTGGATAAAAC+CGG + chr4_2:22219573-22219592 Msa0594420:CDS 35.0%
CTCAATACTAACAACAGGTT+CGG - chr4_2:22219617-22219636 None:intergenic 35.0%
TTCTCCTCAATACTAACAAC+AGG - chr4_2:22219622-22219641 None:intergenic 35.0%
! TGGTTTTCATTCTTCGATTG+AGG - chr4_2:22219814-22219833 None:intergenic 35.0%
! GGTGTTGAACAAAAGTTTGA+GGG + chr4_2:22219949-22219968 Msa0594420:CDS 35.0%
TTTGAGGGTCAATTGAATGA+TGG + chr4_2:22219964-22219983 Msa0594420:CDS 35.0%
TATTGCAGAATTGTGTGTTC+CGG + chr4_2:22220149-22220168 Msa0594420:CDS 35.0%
AAATACTTTCTGATAGCCAC+GGG - chr4_2:22220285-22220304 None:intergenic 35.0%
ATATGCAGCTTGCATGAAAT+TGG + chr4_2:22220429-22220448 Msa0594420:CDS 35.0%
TATGCAGCTTGCATGAAATT+GGG + chr4_2:22220430-22220449 Msa0594420:CDS 35.0%
!! CATCTGAGAGTATCATGTAT+AGG + chr4_2:22220463-22220482 Msa0594420:CDS 35.0%
GTTGGAGATAAATGGAAAAC+TGG + chr4_2:22220509-22220528 Msa0594420:CDS 35.0%
! CAACTACTATTTGAGAGTGT+AGG - chr4_2:22220582-22220601 None:intergenic 35.0%
TGACATTAGGCTCAATAAAC+AGG - chr4_2:22220671-22220690 None:intergenic 35.0%
AATATCCTCTAATTGAAGCC+TGG - chr4_2:22220722-22220741 None:intergenic 35.0%
TCCGAATCACAATTCAACAA+TGG + chr4_2:22220881-22220900 Msa0594420:CDS 35.0%
CCCATTGTTGAATTGTGATT+CGG - chr4_2:22220885-22220904 None:intergenic 35.0%
CCGAATCACAATTCAACAAT+GGG + chr4_2:22220882-22220901 Msa0594420:CDS 35.0%
CACAATTCAACAATGGGATT+AGG + chr4_2:22220888-22220907 Msa0594420:CDS 35.0%
TCAATATCGCGAGAATGAAA+GGG + chr4_2:22220913-22220932 Msa0594420:CDS 35.0%
TTAGAAGGTTCAAAACAAGC+AGG - chr4_2:22221170-22221189 None:intergenic 35.0%
TGAGTTGTACTAATCCCATT+GGG - chr4_2:22221274-22221293 None:intergenic 35.0%
! TTGAGTTGTACTAATCCCAT+TGG - chr4_2:22221275-22221294 None:intergenic 35.0%
TGATATAATCCTGTTCCCAA+AGG - chr4_2:22221587-22221606 None:intergenic 35.0%
GCTTTACAACATGTACACAA+AGG + chr4_2:22221680-22221699 Msa0594420:CDS 35.0%
CACTCTTTGATAGATTGGTA+TGG - chr4_2:22221771-22221790 None:intergenic 35.0%
GGATACACTCTTTGATAGAT+TGG - chr4_2:22221776-22221795 None:intergenic 35.0%
AATATTGCAAGCCAAGCAAA+GGG - chr4_2:22221808-22221827 None:intergenic 35.0%
TAATATTGCAAGCCAAGCAA+AGG - chr4_2:22221809-22221828 None:intergenic 35.0%
ATGCTTGTGGAAAACTTGAA+TGG + chr4_2:22221828-22221847 Msa0594420:CDS 35.0%
GGAAAACTTGAATGGTATCT+TGG + chr4_2:22221836-22221855 Msa0594420:CDS 35.0%
AGCTGTTAAATCATTGACCA+AGG + chr4_2:22221871-22221890 Msa0594420:CDS 35.0%
TACAAAGACTCCATTTGTGA+AGG + chr4_2:22221922-22221941 Msa0594420:CDS 35.0%
AAGCATTAAGCATGCTAGTT+GGG - chr4_2:22221976-22221995 None:intergenic 35.0%
TCTTGCTCTTCACAAACATT+AGG - chr4_2:22222061-22222080 None:intergenic 35.0%
ATGTTTGTGAAGAGCAAGAA+AGG + chr4_2:22222062-22222081 Msa0594420:CDS 35.0%
!! GAAAGCTTTGTTAGACCAGA+TGG + chr4_2:22218721-22218740 Msa0594420:CDS 40.0%
AACAGAAACAATCCGAACAG+AGG + chr4_2:22218788-22218807 Msa0594420:CDS 40.0%
GAGAACCAATTCCGGTTATT+AGG + chr4_2:22218813-22218832 Msa0594420:CDS 40.0%
CAAACCCTAATAACCGGAAT+TGG - chr4_2:22218821-22218840 None:intergenic 40.0%
AGACATGCTTTCAGAATTGC+AGG + chr4_2:22219025-22219044 Msa0594420:CDS 40.0%
TAACTCATGTTCGCGTAAGA+TGG + chr4_2:22219048-22219067 Msa0594420:CDS 40.0%
AACAGATGTCGAAAACGCTT+CGG - chr4_2:22219164-22219183 None:intergenic 40.0%
CTCTAATGAGCATCACATGA+AGG + chr4_2:22219291-22219310 Msa0594420:CDS 40.0%
GGAGTCAGTATCAGAAACAT+TGG + chr4_2:22219468-22219487 Msa0594420:CDS 40.0%
TATCCAACTCACTCTAGGTT+CGG - chr4_2:22219569-22219588 None:intergenic 40.0%
!! GGTTTTATCCAACTCACTCT+AGG - chr4_2:22219574-22219593 None:intergenic 40.0%
GTGACTGAATGTTCTTCAAC+CGG - chr4_2:22219595-22219614 None:intergenic 40.0%
CGAACCTGTTGTTAGTATTG+AGG + chr4_2:22219615-22219634 Msa0594420:CDS 40.0%
TATGCCGGAGATTGTGATTT+CGG + chr4_2:22219708-22219727 Msa0594420:CDS 40.0%
! TTCGATTGAGGATTTGTTCC+AGG - chr4_2:22219802-22219821 None:intergenic 40.0%
!! TTTTTAGCAATCAGGATCCC+TGG + chr4_2:22219911-22219930 Msa0594420:CDS 40.0%
CCGTTATCGTGTATAATCCA+GGG - chr4_2:22219931-22219950 None:intergenic 40.0%
CCCTGGATTATACACGATAA+CGG + chr4_2:22219928-22219947 Msa0594420:CDS 40.0%
ACCGTTATCGTGTATAATCC+AGG - chr4_2:22219932-22219951 None:intergenic 40.0%
! CGGTGTTGAACAAAAGTTTG+AGG + chr4_2:22219948-22219967 Msa0594420:CDS 40.0%
!! TTGAAGTTGTTGGTCTTCCT+TGG - chr4_2:22220040-22220059 None:intergenic 40.0%
!!! CATCTGTTGGTTTTCAGTCA+CGG + chr4_2:22220082-22220101 Msa0594420:CDS 40.0%
CTAGAAATTACCGATGGCTT+TGG - chr4_2:22220123-22220142 None:intergenic 40.0%
TCACTGCTAGAAATTACCGA+TGG - chr4_2:22220129-22220148 None:intergenic 40.0%
ACATTGTCAGAACTACTCAC+TGG - chr4_2:22220357-22220376 None:intergenic 40.0%
!! GAGTAGTTCTGACAATGTGT+TGG + chr4_2:22220359-22220378 Msa0594420:CDS 40.0%
TTATGGAGCAGAGAAGCTTA+TGG + chr4_2:22220400-22220419 Msa0594420:CDS 40.0%
TCATGCAAGCTGCATATTTC+TGG - chr4_2:22220426-22220445 None:intergenic 40.0%
ATGCAGCTTGCATGAAATTG+GGG + chr4_2:22220431-22220450 Msa0594420:CDS 40.0%
TGATACTCTCAGATGTACCA+GGG - chr4_2:22220458-22220477 None:intergenic 40.0%
ATGATACTCTCAGATGTACC+AGG - chr4_2:22220459-22220478 None:intergenic 40.0%
GCATGACCATATTGCATTGT+TGG + chr4_2:22220491-22220510 Msa0594420:CDS 40.0%
GCTGCTTCAATAGTTAATGG+TGG + chr4_2:22220627-22220646 Msa0594420:CDS 40.0%
ATGATTATCGCACCAGATTC+CGG - chr4_2:22220699-22220718 None:intergenic 40.0%
ATCATCCAGGCTTCAATTAG+AGG + chr4_2:22220714-22220733 Msa0594420:CDS 40.0%
! TTGGTTAGAAGGCATGTTGT+TGG + chr4_2:22220807-22220826 Msa0594420:CDS 40.0%
! TTGTGATTCGGAGCTGAAAA+TGG - chr4_2:22220873-22220892 None:intergenic 40.0%
GTCAATATCGCGAGAATGAA+AGG + chr4_2:22220912-22220931 Msa0594420:CDS 40.0%
CAATATCGCGAGAATGAAAG+GGG + chr4_2:22220914-22220933 Msa0594420:CDS 40.0%
AAACTTATATGGGGACCAAC+AGG + chr4_2:22221080-22221099 Msa0594420:CDS 40.0%
GAAGACAAAGACAGTTGCAT+GGG + chr4_2:22221109-22221128 Msa0594420:CDS 40.0%
TTCAGGAAGCAGATTTGAAC+TGG - chr4_2:22221234-22221253 None:intergenic 40.0%
! ATATCATTCCAAGCACTGCT+AGG + chr4_2:22221366-22221385 Msa0594420:CDS 40.0%
! TATCATTCCAAGCACTGCTA+GGG + chr4_2:22221367-22221386 Msa0594420:CDS 40.0%
TAGGGACAACTACAAGTTAG+TGG + chr4_2:22221385-22221404 Msa0594420:CDS 40.0%
GTCAGATATGTGAGCTACTA+AGG - chr4_2:22221414-22221433 None:intergenic 40.0%
TCACATATCTGACATCACAG+AGG + chr4_2:22221421-22221440 Msa0594420:CDS 40.0%
ATGCGCATTACATGCAAACT+TGG - chr4_2:22221540-22221559 None:intergenic 40.0%
TCTCAACTCACTGTCACAAA+TGG - chr4_2:22221564-22221583 None:intergenic 40.0%
CAAGCTGCTGTGATTGATAA+TGG - chr4_2:22221618-22221637 None:intergenic 40.0%
GGCTTGCAATATTATGCTTG+TGG + chr4_2:22221815-22221834 Msa0594420:CDS 40.0%
TGAATGGTATCTTGGAGACA+CGG + chr4_2:22221844-22221863 Msa0594420:CDS 40.0%
TAAATCATTGACCAAGGCTC+TGG + chr4_2:22221877-22221896 Msa0594420:CDS 40.0%
AGCATTAAGCATGCTAGTTG+GGG - chr4_2:22221975-22221994 None:intergenic 40.0%
! GAAGCATTAAGCATGCTAGT+TGG - chr4_2:22221977-22221996 None:intergenic 40.0%
!! AGCTTTGTTAGACCAGATGG+TGG + chr4_2:22218724-22218743 Msa0594420:CDS 45.0%
AGAAACAATCCGAACAGAGG+AGG + chr4_2:22218791-22218810 Msa0594420:CDS 45.0%
! ATTGGTTCTCCTCCTCTGTT+CGG - chr4_2:22218803-22218822 None:intergenic 45.0%
! GAAAGCATGTCTCAAAAGGC+CGG - chr4_2:22219017-22219036 None:intergenic 45.0%
TGTTAATCAGGCACGTCTTG+AGG + chr4_2:22219522-22219541 Msa0594420:CDS 45.0%
GTTAATCAGGCACGTCTTGA+GGG + chr4_2:22219523-22219542 Msa0594420:CDS 45.0%
TTAATCAGGCACGTCTTGAG+GGG + chr4_2:22219524-22219543 Msa0594420:CDS 45.0%
GTGTCGAGTAGTTACTATGC+CGG + chr4_2:22219693-22219712 Msa0594420:CDS 45.0%
ATGCCGGAGATTGTGATTTC+GGG + chr4_2:22219709-22219728 Msa0594420:CDS 45.0%
GAACCCGAAATCACAATCTC+CGG - chr4_2:22219715-22219734 None:intergenic 45.0%
TGCTAATAAATCCAGGCCAG+TGG + chr4_2:22219738-22219757 Msa0594420:CDS 45.0%
CAGCAACAGCTTGAAGTTGT+TGG - chr4_2:22220050-22220069 None:intergenic 45.0%
GAAAGTTCAGCTGCATCTGT+TGG + chr4_2:22220069-22220088 Msa0594420:CDS 45.0%
ACCTACAAAGCCAAAGCCAT+CGG + chr4_2:22220110-22220129 Msa0594420:CDS 45.0%
! AACCATGGATCTGGAGGTTT+TGG - chr4_2:22220183-22220202 None:intergenic 45.0%
GGGGAAATCCTATATCGTGA+AGG + chr4_2:22220215-22220234 Msa0594420:CDS 45.0%
GGTGATCTCCTTCACGATAT+AGG - chr4_2:22220226-22220245 None:intergenic 45.0%
CATTGAAGATCTTGTACCCG+TGG + chr4_2:22220266-22220285 Msa0594420:CDS 45.0%
TGAGCCTAATGTCAATCCTC+CGG + chr4_2:22220677-22220696 Msa0594420:CDS 45.0%
GATTCCGGAGGATTGACATT+AGG - chr4_2:22220684-22220703 None:intergenic 45.0%
AATGTCAATCCTCCGGAATC+TGG + chr4_2:22220684-22220703 Msa0594420:CDS 45.0%
ATCTGGTGCGATAATCATCC+AGG + chr4_2:22220701-22220720 Msa0594420:CDS 45.0%
AAGTTGCAAGCTGTTGTTCG+CGG + chr4_2:22220780-22220799 Msa0594420:CDS 45.0%
TGCAACTGTCTTTGTCTTCC+CGG - chr4_2:22221108-22221127 None:intergenic 45.0%
GGAAGACAAAGACAGTTGCA+TGG + chr4_2:22221108-22221127 Msa0594420:CDS 45.0%
TGACTTCTGTCATCGAGTTC+AGG - chr4_2:22221251-22221270 None:intergenic 45.0%
GATGACAGAAGTCACCCAAT+GGG + chr4_2:22221257-22221276 Msa0594420:CDS 45.0%
!! TGACATCACAGAGGAGCATT+TGG + chr4_2:22221430-22221449 Msa0594420:CDS 45.0%
! TGACAGTGAGTTGAGACCTT+TGG + chr4_2:22221568-22221587 Msa0594420:CDS 45.0%
! GACAGTGAGTTGAGACCTTT+GGG + chr4_2:22221569-22221588 Msa0594420:CDS 45.0%
ACATGTACACAAAGGGACTG+AGG + chr4_2:22221688-22221707 Msa0594420:CDS 45.0%
CCTTAGTATATCCAGAGCCT+TGG - chr4_2:22221891-22221910 None:intergenic 45.0%
!! CCAAGGCTCTGGATATACTA+AGG + chr4_2:22221888-22221907 Msa0594420:CDS 45.0%
TTTGTGGCAGCCTTCACAAA+TGG - chr4_2:22221935-22221954 None:intergenic 45.0%
TGTGAAGGCTGCCACAAATT+TGG + chr4_2:22221937-22221956 Msa0594420:CDS 45.0%
CAAGATCATGTCTGCTCACT+GGG + chr4_2:22222099-22222118 Msa0594420:CDS 45.0%
ACAATTCCAACAATGGGACC+CGG + chr4_2:22222174-22222193 Msa0594420:CDS 45.0%
TCAATGAACAAGAGACCACC+CGG - chr4_2:22222196-22222215 None:intergenic 45.0%
GGTGGTCTCTTGTTCATTGA+CGG + chr4_2:22222196-22222215 Msa0594420:CDS 45.0%
ATTGACGGAGTCAACAAGCA+TGG + chr4_2:22222211-22222230 Msa0594420:CDS 45.0%
TTGACGGAGTCAACAAGCAT+GGG + chr4_2:22222212-22222231 Msa0594420:CDS 45.0%
CAGAGGAGGAGAACCAATTC+CGG + chr4_2:22218805-22218824 Msa0594420:CDS 50.0%
ATGTCTCAAAAGGCCGGTCA+AGG - chr4_2:22219011-22219030 None:intergenic 50.0%
! TGCTGGTTGCAGTCAATTGC+AGG + chr4_2:22219351-22219370 Msa0594420:CDS 50.0%
GCTGGTTGCAGTCAATTGCA+GGG + chr4_2:22219352-22219371 Msa0594420:CDS 50.0%
GCAACCTGATTGTACTCCAC+TGG - chr4_2:22219757-22219776 None:intergenic 50.0%
! GTTGCACACTCGCCTGTTTT+GGG + chr4_2:22219772-22219791 Msa0594420:CDS 50.0%
GTTCCAGGACTTCCCAAAAC+AGG - chr4_2:22219787-22219806 None:intergenic 50.0%
TGTTGGGTTTGGACATGTGC+AGG + chr4_2:22219873-22219892 Msa0594420:CDS 50.0%
!! ACCGATGGCTTTGGCTTTGT+AGG - chr4_2:22220114-22220133 None:intergenic 50.0%
CACCAAAACCTCCAGATCCA+TGG + chr4_2:22220178-22220197 Msa0594420:CDS 50.0%
TTGCATGAAATTGGGGACCC+TGG + chr4_2:22220438-22220457 Msa0594420:CDS 50.0%
ATTATCGCACCAGATTCCGG+AGG - chr4_2:22220696-22220715 None:intergenic 50.0%
TTCGCGGTCACTTGGTTAGA+AGG + chr4_2:22220796-22220815 Msa0594420:CDS 50.0%
ATATGGGGACCAACAGGAAC+CGG + chr4_2:22221086-22221105 Msa0594420:CDS 50.0%
TTTGTCTTCCCGGTTCCTGT+TGG - chr4_2:22221098-22221117 None:intergenic 50.0%
CGATGACAGAAGTCACCCAA+TGG + chr4_2:22221256-22221275 Msa0594420:CDS 50.0%
TAGTTGTCCCTAGCAGTGCT+TGG - chr4_2:22221377-22221396 None:intergenic 50.0%
! GAGTTGAGACCTTTGGGAAC+AGG + chr4_2:22221575-22221594 Msa0594420:CDS 50.0%
CAGCTTGCCCGATTCAATGT+TGG + chr4_2:22221631-22221650 Msa0594420:CDS 50.0%
ATGGTATCTTGGAGACACGG+TGG + chr4_2:22221847-22221866 Msa0594420:CDS 50.0%
ATGCTTCCTGCGCACATCAT+AGG + chr4_2:22221990-22222009 Msa0594420:CDS 50.0%
TGCTTCCTGCGCACATCATA+GGG + chr4_2:22221991-22222010 Msa0594420:CDS 50.0%
GCAAGATCATGTCTGCTCAC+TGG + chr4_2:22222098-22222117 Msa0594420:CDS 50.0%
TCACTGGGAGCTCAAGACTA+AGG + chr4_2:22222114-22222133 Msa0594420:CDS 50.0%
CAATTCCAACAATGGGACCC+GGG + chr4_2:22222175-22222194 Msa0594420:CDS 50.0%
CAATGAACAAGAGACCACCC+GGG - chr4_2:22222195-22222214 None:intergenic 50.0%
! TGTTAGACCAGATGGTGGCG+TGG + chr4_2:22218729-22218748 Msa0594420:CDS 55.0%
GAAGGTAGCTGCCGAAGCTA+TGG + chr4_2:22219309-22219328 Msa0594420:CDS 55.0%
TCGCCGAACCTAGAGTGAGT+TGG + chr4_2:22219563-22219582 Msa0594420:CDS 55.0%
CTGATTGTACTCCACTGGCC+TGG - chr4_2:22219752-22219771 None:intergenic 55.0%
CAGGCCAGTGGAGTACAATC+AGG + chr4_2:22219750-22219769 Msa0594420:CDS 55.0%
! GGTTGCACACTCGCCTGTTT+TGG + chr4_2:22219771-22219790 Msa0594420:CDS 55.0%
!!! TCGCCTGTTTTGGGAAGTCC+TGG + chr4_2:22219781-22219800 Msa0594420:CDS 55.0%
!! GGATCTGGAGGTTTTGGTGC+TGG - chr4_2:22220177-22220196 None:intergenic 55.0%
GTCGAGAACCATGGATCTGG+AGG - chr4_2:22220189-22220208 None:intergenic 55.0%
GTCGTCGAGAACCATGGATC+TGG - chr4_2:22220192-22220211 None:intergenic 55.0%
TCCATGGTTCTCGACGACGA+CGG + chr4_2:22220194-22220213 Msa0594420:CDS 55.0%
AGCTGTTGTTCGCGGTCACT+TGG + chr4_2:22220788-22220807 Msa0594420:CDS 55.0%
TATGGGGACCAACAGGAACC+GGG + chr4_2:22221087-22221106 Msa0594420:CDS 55.0%
CCAAGCAAAGGGTGAACAGC+TGG - chr4_2:22221797-22221816 None:intergenic 55.0%
CCAGCTGTTCACCCTTTGCT+TGG + chr4_2:22221794-22221813 Msa0594420:CDS 55.0%
TGATGCCCTATGATGTGCGC+AGG - chr4_2:22221999-22222018 None:intergenic 55.0%
TAGCTGTCCACGCCACCATC+TGG - chr4_2:22218739-22218758 None:intergenic 60.0%
! GATGGTGGCGTGGACAGCTA+AGG + chr4_2:22218739-22218758 Msa0594420:CDS 60.0%
CCATGGTTCTCGACGACGAC+GGG + chr4_2:22220195-22220214 Msa0594420:CDS 60.0%
CATGGTTCTCGACGACGACG+GGG + chr4_2:22220196-22220215 Msa0594420:CDS 60.0%
TTCCAACAATGGGACCCGGG+TGG + chr4_2:22222178-22222197 Msa0594420:CDS 60.0%
!!! GGAGGTTTTGGTGCTGGCGC+CGG - chr4_2:22220171-22220190 None:intergenic 65.0%
CCCGTCGTCGTCGAGAACCA+TGG - chr4_2:22220198-22220217 None:intergenic 65.0%
GACCACCCGGGTCCCATTGT+TGG - chr4_2:22222183-22222202 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 22218719 22222252 22218719 ID=Msa0594420;Name=Msa0594420
chr4_2 mRNA 22218719 22222252 22218719 ID=Msa0594420-mRNA-1;Parent=Msa0594420;Name=Msa0594420-mRNA-1;_AED=0.34;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|1177
chr4_2 exon 22218719 22222252 22218719 ID=Msa0594420-mRNA-1:exon:6050;Parent=Msa0594420-mRNA-1
chr4_2 CDS 22218719 22222252 22218719 ID=Msa0594420-mRNA-1:cds;Parent=Msa0594420-mRNA-1
Gene Sequence

>Msa0594420

ATGAAAGCTTTGTTAGACCAGATGGTGGCGTGGACAGCTAAGGTTTACAACAACAACGCCAACAACAACAACAGAAACAATCCGAACAGAGGAGGAGAACCAATTCCGGTTATTAGGGTTTGTAATAACATCCCTACTATTGTTACGTACCGAAAATCAAAATATGCCAATGAATCTGATTTGGAATACTATGAGCGTAAATATTACACAAAGTTGAAGGATGATTACTACCAATTTGAAATCTACGATTCAGTATATCGTTGTCCGTTTTGTTACAACAAAGATTACTCCTTGACCGGCCTTTTGAGACATGCTTTCAGAATTGCAGGTAACTCATGTTCGCGTAAGATGGTTAAAGATATTGCTCGACATTCTGTTTTGATAACATATATTCTAAGAATTCTTACGACGTTGAAAGAGGAGATCGACGTTGCTCAGACGACCGAAGCGTTTTCGACATCTGTTTCGACTAATGCAAGTTCTTGTGAAGAAAGCTTGGTAATGGCTGCGACAATTGAATCAAAAGATGGAGATAAAGTTCAAGAAGTGGGTTGTAGAACATCTGACACAATCTCTAATGAGCATCACATGAAGGTAGCTGCCGAAGCTATGGCTAAAATTACAGAATCGGTTGCTGGTTGCAGTCAATTGCAGGGTGATGTTGAAAACAAAGTTCTAGGAGGCTCATCTCTCTCCACCAAAAAGCATTCAATTTCTGTGCCAGTTTATGTAGATGAATTAGTAAAGGTGGAGTCAGTATCAGAAACATTGGGTACAGAAATTGTACTTGAAATTGCACTTCATGTTAATCAGGCACGTCTTGAGGGGTTAGTAGTACATTCAGTCGCCGAACCTAGAGTGAGTTGGATAAAACCGGTTGAAGAACATTCAGTCACCGAACCTGTTGTTAGTATTGAGGAGAAGTTGAATGTTCAAAATTTAAATGATGCAAAAGAAGGCACTCATCATGTTCAGTGTCGAGTAGTTACTATGCCGGAGATTGTGATTTCGGGTTCATATGCTAATAAATCCAGGCCAGTGGAGTACAATCAGGTTGCACACTCGCCTGTTTTGGGAAGTCCTGGAACAAATCCTCAATCGAAGAATGAAAACCATCAGAAGAATGATGCAATTTTGTCTTCAATTTCTAAACATGTTGGGTTTGGACATGTGCAGGATTCTTCAAACTCACTTTTTAGCAATCAGGATCCCTGGATTATACACGATAACGGTGTTGAACAAAAGTTTGAGGGTCAATTGAATGATGGTCTCTATCAGACATTCAAACAAAATTTTACATTAAAAGAAGTTCGATATACCAAGGAAGACCAACAACTTCAAGCTGTTGCTGAAAGTTCAGCTGCATCTGTTGGTTTTCAGTCACGGAGTCTACCTACAAAGCCAAAGCCATCGGTAATTTCTAGCAGTGATATTGCAGAATTGTGTGTTCCGGCGCCAGCACCAAAACCTCCAGATCCATGGTTCTCGACGACGACGGGGAAATCCTATATCGTGAAGGAGATCACCACTACTGATTTTTCATTATGCATTGAAGATCTTGTACCCGTGGCTATCAGAAAGTATTTAAAAGCTATGATTTCAACGATGCGTCATTCTCAGACTATTGCTTCTGTTCCAGTGAGTAGTTCTGACAATGTGTTGGAGAGAATGTTTGCTTTATTTATGGAGCAGAGAAGCTTATGGCCAGAAATATGCAGCTTGCATGAAATTGGGGACCCTGGTACATCTGAGAGTATCATGTATAGGTATCTGCATGACCATATTGCATTGTTGGAGATAAATGGAAAACTGGAAACTTTTGAAACAATTAATGAGAAGATTCGAAAATGCTTTAAGAATCCTACACTCTCAAATAGTAGTTGTGCAAAAGTTTGTAAGCATACTTATGCTGCTTCAATAGTTAATGGTGGTTCAGTTGATGAAACAAACCTGTTTATTGAGCCTAATGTCAATCCTCCGGAATCTGGTGCGATAATCATCCAGGCTTCAATTAGAGGATATTTGATTCGGAGAGCACTTCTAAAGAGTAAGAATGTAGTGAAGTTGCAAGCTGTTGTTCGCGGTCACTTGGTTAGAAGGCATGTTGTTGGAGCATCGCGATGTATTCAAGCTATTACTAAAATGCAAGCTCCATTTTCAGCTCCGAATCACAATTCAACAATGGGATTAGGAGTCAATATCGCGAGAATGAAAGGGGAGAAATTTAAGGAAGATGATTGGGGTTTTATCGAGCAATCTTCCTTTGTTAAAGACGTAATTCGTTCTCAGAATCTGAATGAATCACAGCAAGAAGCAATTTCAAGCTGTGTTAGTATGATGAATTGTTATCATGCTAATGTCAAACTTATATGGGGACCAACAGGAACCGGGAAGACAAAGACAGTTGCATGGGAAAACAGTCTTGATGATTGTAAAAAAGAAAGTATTCCTGCTTGTTTTGAACCTTCTAACTCTGAAATAGATCATTTCCTTAGAATGCTAAGTTTGCTTTCCAGTTCAAATCTGCTTCCTGAACTCGATGACAGAAGTCACCCAATGGGATTAGTACAACTCAATGCAATGCGTCTAGCTGACATTTTCTCCGATAATTCAAATTTTCGATCTTACATGATACTTTGTTTTACTGATATCATTCCAAGCACTGCTAGGGACAACTACAAGTTAGTGGAGACCTTAGTAGCTCACATATCTGACATCACAGAGGAGCATTTGGTGTTATATGCACAAATGGCTAATATTTTACATGTAATACTACAATGGCCTGAAATCAATATTAAAGAGATTGCAGAATTTTTTTCCAAGTTTGCATGTAATGCGCATACCATTTGTGACAGTGAGTTGAGACCTTTGGGAACAGGATTATATCATGTTGTTTCCATTATCAATCACAGCAGCTTGCCCGATTCAATGTTGGTTTTTGAAGGAAGAGTAGCAGTACGTGCTTTACAACATGTACACAAAGGGACTGAGGTATTGAAATCAGTAAAGCTAGAATATTGGAGAGAAGCTTTAGCATATTGCAAATTGACCATACCAATCTATCAAAGAGTGTATCCAGCTGTTCACCCTTTGCTTGGCTTGCAATATTATGCTTGTGGAAAACTTGAATGGTATCTTGGAGACACGGTGGAAGCTGTTAAATCATTGACCAAGGCTCTGGATATACTAAGGATTACTCATGGTACAAAGACTCCATTTGTGAAGGCTGCCACAAATTTGGAATTCAATCTAACCCCAACTAGCATGCTTAATGCTTCCTGCGCACATCATAGGGCATCATTATTTGTCAACTTCTTTGCAAATCTTCATTGTTTTGTTCCTAATGTTTGTGAAGAGCAAGAAAGGAACTTTTTCGTTCGCAAGATCATGTCTGCTCACTGGGAGCTCAAGACTAAGGAATTAGAATCACAGTTGGTATCCATAATTTCTACTTTACAATTCCAACAATGGGACCCGGGTGGTCTCTTGTTCATTGACGGAGTCAACAAGCATGGGAGCTCTTTATCAAAATGA
Protein sequence

>Msa0594420

MKALLDQMVAWTAKVYNNNANNNNRNNPNRGGEPIPVIRVCNNIPTIVTYRKSKYANESDLEYYERKYYTKLKDDYYQFEIYDSVYRCPFCYNKDYSLTGLLRHAFRIAGNSCSRKMVKDIARHSVLITYILRILTTLKEEIDVAQTTEAFSTSVSTNASSCEESLVMAATIESKDGDKVQEVGCRTSDTISNEHHMKVAAEAMAKITESVAGCSQLQGDVENKVLGGSSLSTKKHSISVPVYVDELVKVESVSETLGTEIVLEIALHVNQARLEGLVVHSVAEPRVSWIKPVEEHSVTEPVVSIEEKLNVQNLNDAKEGTHHVQCRVVTMPEIVISGSYANKSRPVEYNQVAHSPVLGSPGTNPQSKNENHQKNDAILSSISKHVGFGHVQDSSNSLFSNQDPWIIHDNGVEQKFEGQLNDGLYQTFKQNFTLKEVRYTKEDQQLQAVAESSAASVGFQSRSLPTKPKPSVISSSDIAELCVPAPAPKPPDPWFSTTTGKSYIVKEITTTDFSLCIEDLVPVAIRKYLKAMISTMRHSQTIASVPVSSSDNVLERMFALFMEQRSLWPEICSLHEIGDPGTSESIMYRYLHDHIALLEINGKLETFETINEKIRKCFKNPTLSNSSCAKVCKHTYAASIVNGGSVDETNLFIEPNVNPPESGAIIIQASIRGYLIRRALLKSKNVVKLQAVVRGHLVRRHVVGASRCIQAITKMQAPFSAPNHNSTMGLGVNIARMKGEKFKEDDWGFIEQSSFVKDVIRSQNLNESQQEAISSCVSMMNCYHANVKLIWGPTGTGKTKTVAWENSLDDCKKESIPACFEPSNSEIDHFLRMLSLLSSSNLLPELDDRSHPMGLVQLNAMRLADIFSDNSNFRSYMILCFTDIIPSTARDNYKLVETLVAHISDITEEHLVLYAQMANILHVILQWPEINIKEIAEFFSKFACNAHTICDSELRPLGTGLYHVVSIINHSSLPDSMLVFEGRVAVRALQHVHKGTEVLKSVKLEYWREALAYCKLTIPIYQRVYPAVHPLLGLQYYACGKLEWYLGDTVEAVKSLTKALDILRITHGTKTPFVKAATNLEFNLTPTSMLNASCAHHRASLFVNFFANLHCFVPNVCEEQERNFFVRKIMSAHWELKTKELESQLVSIISTLQFQQWDPGGLLFIDGVNKHGSSLSK*