AlfalfaGEDB | Msa0596500

M. sativa cultivar zhongmu-4 / Msa0596500

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009889 GO:0010035 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0596500	TKY57837.1	45.930	172	76	4	3	158	8	178	1.16e-40	149

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0596500	sp\|Q9C8W9\|NAC30_ARATH	30.189	159	80	6	4	139	9	159	2.30e-11	65.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0596500	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0596500	MtrunA17_Chr1g0200561	27.869	183	101	6	4	163	19	193	1.00e-12	65.9
Msa0596500	MtrunA17_Chr5g0398971	29.936	157	79	6	4	136	7	156	1.39e-12	65.1
Msa0596500	MtrunA17_Chr4g0056541	30.769	156	77	6	4	136	7	154	5.28e-12	63.5
Msa0596500	MtrunA17_Chr5g0405211	29.936	157	77	7	4	136	8	155	5.90e-12	63.5
Msa0596500	MtrunA17_Chr4g0017921	30.508	177	89	10	4	156	8	174	1.05e-11	62.8
Msa0596500	MtrunA17_Chr1g0147421	31.126	151	87	5	2	136	25	174	1.17e-11	62.8
Msa0596500	MtrunA17_Chr4g0018391	31.410	156	76	7	4	136	7	154	1.40e-11	62.4
Msa0596500	MtrunA17_Chr1g0183741	33.113	151	77	9	4	136	10	154	3.60e-11	60.8
Msa0596500	MtrunA17_Chr3g0109821	30.323	155	81	7	4	136	6	155	4.10e-11	61.2
Msa0596500	MtrunA17_Chr7g0261411	33.557	149	79	8	4	136	10	154	4.46e-11	60.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0596500	AT2G27300.1	31.293	147	79	5	4	135	14	153	1.43e-13	68.6
Msa0596500	AT3G17730.1	31.613	155	78	8	4	136	6	154	2.31e-13	67.0
Msa0596500	AT1G71930.2	30.189	159	80	6	4	139	9	159	2.34e-12	65.1
Msa0596500	AT1G71930.1	30.189	159	80	6	4	139	9	159	2.34e-12	65.1
Msa0596500	AT3G49530.2	28.571	154	91	6	1	136	10	162	2.37e-12	65.1
Msa0596500	AT3G49530.1	28.571	154	91	6	1	136	10	162	2.98e-12	65.1
Msa0596500	AT1G62700.1	30.769	156	77	7	4	136	7	154	3.02e-12	64.7
Msa0596500	AT1G62700.2	30.769	156	77	7	4	136	7	154	3.02e-12	64.7
Msa0596500	AT4G36160.2	30.573	157	76	7	4	136	14	161	4.23e-12	64.3
Msa0596500	AT4G36160.1	30.573	157	76	7	4	136	10	157	4.23e-12	64.3

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Find 44 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGCGGCAAACAACAAA+AGG	0.449123	4_2:-26863849	None:intergenic
AACAAGGAGCTCATCTGAAG+GGG	0.618835	4_2:-26863363	None:intergenic
AAGGAACTCAGCAACTTAAG+GGG	0.605135	4_2:+26863973	Msa0596500:CDS
ACCTTCATCGACATCAGAAA+AGG	0.512248	4_2:-26863913	None:intergenic
AGAACGCGATCAAAACTAGC+TGG	0.546737	4_2:+26863681	Msa0596500:CDS
AGAGGAGGCATTGTAAGATT+GGG	0.450614	4_2:+26863558	Msa0596500:CDS
AGGAACTCAGCAACTTAAGG+GGG	0.676579	4_2:+26863974	Msa0596500:CDS
AGGAGTGGCTTGAGTAGCCA+TGG	0.558328	4_2:-26864039	None:intergenic
AGGCTATGCATCGCATTCCA+TGG	0.571087	4_2:+26864022	Msa0596500:CDS
AGTAAAATTCCCTTGGGTGA+CGG	0.539767	4_2:-26863511	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCCAACAAAAACAATGAAG+AGG	+	chr4_2:26863397-26863416	Msa0596500:CDS	35.0%
GAAGAGGTTCAGAATTTCAA+TGG	+	chr4_2:26863413-26863432	Msa0596500:CDS	35.0%
ATAAGTTCAGCTCCTTAATC+AGG	-	chr4_2:26863445-26863464	None:intergenic	35.0%
TGAAGCAGTAAAATTCCCTT+GGG	-	chr4_2:26863520-26863539	None:intergenic	35.0%
TAACAAGGAGCTCATCTGAA+GGG	-	chr4_2:26863367-26863386	None:intergenic	40.0%
GGACTAAGAGGCAATTCTAA+TGG	-	chr4_2:26863479-26863498	None:intergenic	40.0%
AGTAAAATTCCCTTGGGTGA+CGG	-	chr4_2:26863514-26863533	None:intergenic	40.0%
GTGAAGCAGTAAAATTCCCT+TGG	-	chr4_2:26863521-26863540	None:intergenic	40.0%
TTCCCAAATGACATACCTGA+GGG	-	chr4_2:26863736-26863755	None:intergenic	40.0%
TTTCCCAAATGACATACCTG+AGG	-	chr4_2:26863737-26863756	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	26863332	26864064	26863332	ID=Msa0596500;Name=Msa0596500
chr4_2	mRNA	26863332	26864064	26863332	ID=Msa0596500-mRNA-1;Parent=Msa0596500;Name=Msa0596500-mRNA-1;_AED=0.27;_eAED=0.31;_QI=0\|0\|0\|0.66\|0.5\|0.33\|3\|0\|199
chr4_2	exon	26863332	26863468	26863332	ID=Msa0596500-mRNA-1:exon:7136;Parent=Msa0596500-mRNA-1
chr4_2	exon	26863496	26863739	26863496	ID=Msa0596500-mRNA-1:exon:7137;Parent=Msa0596500-mRNA-1
chr4_2	exon	26863846	26864064	26863846	ID=Msa0596500-mRNA-1:exon:7138;Parent=Msa0596500-mRNA-1
chr4_2	CDS	26863332	26863468	26863332	ID=Msa0596500-mRNA-1:cds;Parent=Msa0596500-mRNA-1
chr4_2	CDS	26863496	26863739	26863496	ID=Msa0596500-mRNA-1:cds;Parent=Msa0596500-mRNA-1
chr4_2	CDS	26863846	26864064	26863846	ID=Msa0596500-mRNA-1:cds;Parent=Msa0596500-mRNA-1

Gene Sequence

>Msa0596500

ATGGAATTCCTTCCACCTGGTGTTGGCTTCGCCCCTTCAGATGAGCTCCTTGTTAGCTACTACCTTGCCAACAAAAACAATGAAGAGGTTCAGAATTTCAATGGTTCTGACCTGATTAAGGAGCTGAACTTATACCATCCATCATTTTTCTTCCGTCACCCAAGGGAATTTTACTGCTTCACAGCCAAAGTTGATGAGCAGAGGAGGCATTGTAAGATTGGGTTTTGGATGCGTAAGGGAGTTGTTCGAGACATTTGTGGAGTTGATAACGTGGTTGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCCCTCAGAACGCGATCAAAACTAGCTGGACTATGTACGAGTACACTCAGGTTAACAACCCTCAGGCTCCTTTTGTTGTTTGCCGCGTTTTTGACTGCCCTAGAGGGATGGATCTGGACTCTGAATTTGGAGCCTTTTCTGATGTCGATGAAGGTGATTATGCTGATGACGACTTCGATGACAAGGCGAATGAAGGAACTCAGCAACTTAAGGGGGCTGGGGCTAGTTCTAGTGCTGAGCAAGGCTATGCATCGCATTCCATGGCTACTCAAGCCACTCCTTAG

Protein sequence

>Msa0596500

MEFLPPGVGFAPSDELLVSYYLANKNNEEVQNFNGSDLIKELNLYHPSFFFRHPREFYCFTAKVDEQRRHCKIGFWMRKGVVRDICGVDNVVVGKKSLFMFYIGNSPQNAIKTSWTMYEYTQVNNPQAPFVVCRVFDCPRGMDLDSEFGAFSDVDEGDYADDDFDDKANEGTQQLKGAGASSSAEQGYASHSMATQATP*