AlfalfaGEDB | Msa0597420

M. sativa cultivar zhongmu-4 / Msa0597420

KEGG: K06627
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009888 GO:0009987 GO:0010015 GO:0010101 GO:0010102 GO:0010311 GO:0010374 GO:0010389 GO:0010440 GO:0010444 GO:0010564 GO:0022402 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048364 GO:0048518 GO:0048527 GO:0048528 GO:0048580 GO:0048582 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051239 GO:0051240 GO:0051726 GO:0065007 GO:0071704 GO:0090304 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1901576 GO:1901987 GO:1901990 GO:1902749 GO:1905392 GO:1905393 GO:2000026 GO:2000038 GO:2000123
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0597420	CAA59768.1	100.000	63	0	0	68	130	390	452	2.73e-32	129

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0597420	sp\|Q38819\|CCA23_ARATH	69.841	63	18	1	68	130	389	450	2.78e-20	88.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0597420	MtrunA17_Chr2g0332171	90.625	64	5	1	68	130	422	485	1.05e-31	117
Msa0597420	MtrunA17_Chr2g0332181	76.190	63	14	1	68	130	368	429	3.15e-23	93.2
Msa0597420	MtrunA17_Chr1g0148681	60.317	63	23	2	68	130	357	417	1.30e-14	68.9
Msa0597420	MtrunA17_Chr3g0138431	57.377	61	24	2	70	130	416	474	2.58e-13	65.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0597420	AT1G15570.1	69.841	63	18	1	68	130	389	450	2.82e-21	88.2
Msa0597420	AT1G80370.1	63.492	63	22	1	68	130	398	459	3.19e-17	77.0
Msa0597420	AT5G11300.1	52.381	63	29	1	68	130	373	434	3.00e-14	68.6
Msa0597420	AT5G25380.3	49.206	63	31	1	68	130	374	435	5.58e-11	58.9
Msa0597420	AT5G25380.1	49.206	63	31	1	68	130	374	435	5.58e-11	58.9
Msa0597420	AT5G25380.4	49.206	63	31	1	68	130	380	441	5.59e-11	58.9

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Find 39 sgRNAs with CRISPR-Local

Find 783 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTGTTCTGAATATGA+TGG	0.489279	4_2:+29649971	Msa0597420:exon
AACAAGTGAAGATTTGCGAA+AGG	0.585275	4_2:-29639550	None:intergenic
AACAATGTTTCAAGCAGTTT+TGG	0.255031	4_2:-29649958	None:intergenic
AAGGTCACTAAGGGTGTGTT+TGG	0.420909	4_2:-29650263	None:intergenic
ACAAGTGAAGATTTGCGAAA+GGG	0.547473	4_2:-29639549	None:intergenic
ACCCTTAGTGACCTTATTAT+GGG	0.375444	4_2:+29650271	Msa0597420:three_prime_UTR
ACCTGAGTCAAGAACGTGAA+AGG	0.553194	4_2:-29639602	None:intergenic
ACTCAGGTTTGGATTGATGG+AGG	0.559679	4_2:+29639617	Msa0597420:CDS
ATAAATCAGTTGCAAATTAG+GGG	0.638054	4_2:-29650015	None:intergenic
ATCTGAAATCAAGACATTCT+AGG	0.364711	4_2:-29650342	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGTGATGAAATAATTGGTTA+GGG	+	chr4_2:29639805-29639824	Msa0597420:intron	30.0%
TAGCCTATAAATTGGTCAAA+GGG	-	chr4_2:29639972-29639991	None:intergenic	30.0%
TTAGCCTATAAATTGGTCAA+AGG	-	chr4_2:29639973-29639992	None:intergenic	30.0%
CAATGACTTAGCCTATAAAT+TGG	-	chr4_2:29639980-29639999	None:intergenic	30.0%
TGGACTCTAACAAACTAAAA+AGG	+	chr4_2:29640084-29640103	Msa0597420:intron	30.0%
TAAGTCATTTGATATGTCTC+TGG	-	chr4_2:29640791-29640810	None:intergenic	30.0%
CCAAAATTAGGGTATAACAA+TGG	+	chr4_2:29640850-29640869	Msa0597420:intron	30.0%
TTCTCATTACTTGTAACTGA+AGG	+	chr4_2:29640951-29640970	Msa0597420:intron	30.0%
CATGGTTGAATATTAGTAGA+TGG	+	chr4_2:29641232-29641251	Msa0597420:intron	30.0%
GTCTCTAAATCAATTCTAAC+TGG	+	chr4_2:29641910-29641929	Msa0597420:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	29639346	29650417	29639346	ID=Msa0597420;Name=Msa0597420
chr4_2	mRNA	29639346	29650417	29639346	ID=Msa0597420-mRNA-1;Parent=Msa0597420;Name=Msa0597420-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|0.25\|0.2\|0.4\|0.75\|1\|5\|433\|130
chr4_2	exon	29639346	29639350	29639346	ID=Msa0597420-mRNA-1:exon:7554;Parent=Msa0597420-mRNA-1
chr4_2	exon	29639532	29639638	29639532	ID=Msa0597420-mRNA-1:exon:7555;Parent=Msa0597420-mRNA-1
chr4_2	exon	29642251	29642339	29642251	ID=Msa0597420-mRNA-1:exon:7556;Parent=Msa0597420-mRNA-1
chr4_2	exon	29644229	29644366	29644229	ID=Msa0597420-mRNA-1:exon:7557;Parent=Msa0597420-mRNA-1
chr4_2	exon	29649931	29650417	29649931	ID=Msa0597420-mRNA-1:exon:7558;Parent=Msa0597420-mRNA-1
chr4_2	CDS	29639346	29639350	29639346	ID=Msa0597420-mRNA-1:cds;Parent=Msa0597420-mRNA-1
chr4_2	CDS	29639532	29639638	29639532	ID=Msa0597420-mRNA-1:cds;Parent=Msa0597420-mRNA-1
chr4_2	CDS	29642251	29642339	29642251	ID=Msa0597420-mRNA-1:cds;Parent=Msa0597420-mRNA-1
chr4_2	CDS	29644229	29644366	29644229	ID=Msa0597420-mRNA-1:cds;Parent=Msa0597420-mRNA-1
chr4_2	CDS	29649931	29649984	29649931	ID=Msa0597420-mRNA-1:cds;Parent=Msa0597420-mRNA-1
chr4_2	three_prime_UTR	29649985	29650417	29649985	ID=Msa0597420-mRNA-1:three_prime_utr;Parent=Msa0597420-mRNA-1

Gene Sequence

>Msa0597420

ATGGCCTTCGTTTTCACCACTTCCCTTTCGCAAATCTTCACTTGTTACGGTTTGATTGGTACGGTTCGAGTTCCTCCTTTCACGTTCTTGACTCAGGTTTGGATTGATGGAGCTAATGTGTTTCATCGTGATTTGAAGCCAAAAAATTATCTTGGTAATGTTGATCGAGAACTCAAGAAATGTGATTTTGGACTTGCTCAAAATCCAACTCTTGAACACTATGCCTCTTACAAAGCATCAGATTTGAAAGCTACAGTTCTTGCATTACAAGATCTACAGCTGAATAGTAATGATGACTGCCCTTTAACCACCATCCGCAAGAAGTACACACAAGACAAGCTCAATTGTGTAGCAGCTTTATCTTCACCAAAACTGCTTGAAACATTGTTCTGA

Protein sequence

>Msa0597420

MAFVFTTSLSQIFTCYGLIGTVRVPPFTFLTQVWIDGANVFHRDLKPKNYLGNVDRELKKCDFGLAQNPTLEHYASYKASDLKATVLALQDLQLNSNDDCPLTTIRKKYTQDKLNCVAALSSPKLLETLF*