AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0599710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0599710 MtrunA17_Chr4g0025061 74.949 491 106 4 8 487 16 500 0.0 712
Msa0599710 MtrunA17_Chr4g0025051 77.991 468 98 1 24 486 7 474 0.0 696
Msa0599710 MtrunA17_Chr4g0024391 85.753 365 52 0 124 488 6 370 0.0 627
Msa0599710 MtrunA17_Chr2g0322391 39.101 445 179 12 63 486 118 491 3.53e-76 250
Msa0599710 MtrunA17_Chr4g0024401 83.193 119 14 2 1 119 1 113 6.15e-58 187
Msa0599710 MtrunA17_Chr4g0075201 34.475 438 180 10 52 470 41 390 1.25e-56 196
Msa0599710 MtrunA17_Chr2g0288871 48.047 256 111 5 245 485 192 440 2.14e-54 191
Msa0599710 MtrunA17_Chr3g0099641 40.426 282 138 7 209 487 139 393 8.95e-54 187
Msa0599710 MtrunA17_Chr7g0270831 59.722 144 55 2 332 473 201 343 1.93e-45 164
Msa0599710 MtrunA17_Chr2g0302391 50.980 153 66 2 327 472 191 341 2.79e-39 147
Msa0599710 MtrunA17_Chr7g0216891 48.387 124 58 1 304 421 170 293 4.28e-32 129
Msa0599710 MtrunA17_Chr4g0051181 58.416 101 42 0 332 432 228 328 1.49e-29 121
Msa0599710 MtrunA17_Chr4g0060271 60.526 76 30 0 332 407 240 315 5.32e-27 114
Msa0599710 MtrunA17_Chr5g0419241 67.925 53 17 0 325 377 55 107 1.12e-21 90.1
Msa0599710 MtrunA17_Chr7g0218081 42.241 116 56 2 304 419 377 481 4.94e-21 95.9
Msa0599710 MtrunA17_Chr5g0418191 43.636 110 53 3 304 409 277 381 5.10e-20 92.4
Msa0599710 MtrunA17_Chr4g0042831 46.914 81 42 1 329 409 260 339 7.76e-19 88.2
Msa0599710 MtrunA17_Chr3g0101131 38.532 109 63 2 320 425 388 495 1.24e-18 89.0
Msa0599710 MtrunA17_Chr5g0445311 43.956 91 50 1 319 409 336 425 2.81e-18 87.8
Msa0599710 MtrunA17_Chr4g0001361 32.450 151 78 4 324 474 139 265 3.57e-18 85.5
Msa0599710 MtrunA17_Chr3g0079691 48.148 81 41 1 329 409 477 556 5.82e-18 87.0
Msa0599710 MtrunA17_Chr7g0249311 46.914 81 42 1 329 409 374 453 3.39e-17 84.7
Msa0599710 MtrunA17_Chr7g0243891 48.148 81 41 1 329 409 356 435 3.45e-17 84.3
Msa0599710 MtrunA17_Chr7g0249301 50.000 76 37 1 334 409 279 353 4.79e-17 82.8
Msa0599710 MtrunA17_Chr3g0106491 39.604 101 60 1 309 409 327 426 6.25e-17 83.2
Msa0599710 MtrunA17_Chr5g0432161 41.935 93 53 1 317 409 391 482 8.46e-17 83.2
Msa0599710 MtrunA17_Chr4g0004911 46.835 79 41 1 329 407 318 395 1.85e-16 82.0
Msa0599710 MtrunA17_Chr3g0136961 38.739 111 61 2 326 431 117 225 4.11e-16 79.0
Msa0599710 MtrunA17_Chr7g0270051 37.273 110 64 2 326 433 30 136 7.44e-16 77.8
Msa0599710 MtrunA17_Chr1g0150271 45.455 66 36 0 342 407 96 161 8.32e-16 77.4
Msa0599710 MtrunA17_Chr2g0319961 44.304 79 44 0 338 416 71 149 8.79e-16 77.4
Msa0599710 MtrunA17_Chr8g0379461 48.485 66 34 0 344 409 73 138 9.90e-16 77.0
Msa0599710 MtrunA17_Chr2g0333851 43.590 78 42 1 333 408 153 230 1.20e-15 77.8
Msa0599710 MtrunA17_Chr5g0447701 40.217 92 54 1 318 409 223 313 1.33e-15 78.6
Msa0599710 MtrunA17_Chr2g0293481 30.636 173 100 5 264 417 166 337 1.38e-15 79.0
Msa0599710 MtrunA17_Chr7g0270191 41.489 94 50 2 332 425 130 218 1.82e-15 77.0
Msa0599710 MtrunA17_Chr4g0051881 39.560 91 54 1 334 424 165 254 2.01e-15 77.4
Msa0599710 MtrunA17_Chr2g0284321 50.000 64 32 0 344 407 32 95 2.60e-15 75.1
Msa0599710 MtrunA17_Chr1g0150281 40.217 92 55 0 332 423 125 216 3.29e-15 76.3
Msa0599710 MtrunA17_Chr8g0386241 34.259 108 70 1 306 413 62 168 5.40e-15 73.2
Msa0599710 MtrunA17_Chr4g0068541 43.421 76 42 1 333 408 194 268 7.00e-15 76.6
Msa0599710 MtrunA17_Chr5g0443131 44.872 78 42 1 334 411 144 220 9.02e-15 75.1
Msa0599710 MtrunA17_Chr2g0294231 41.250 80 46 1 332 411 188 266 1.07e-14 75.9
Msa0599710 MtrunA17_Chr7g0224801 48.571 70 35 1 337 406 83 151 2.37e-14 71.2
Msa0599710 MtrunA17_Chr7g0263531 43.210 81 45 1 327 407 341 420 2.39e-14 75.1
Msa0599710 MtrunA17_Chr7g0248691 45.333 75 41 0 343 417 150 224 5.39e-14 73.2
Msa0599710 MtrunA17_Chr8g0387501 44.444 72 40 0 346 417 128 199 7.18e-14 72.0
Msa0599710 MtrunA17_Chr1g0194481 41.026 78 45 1 334 411 145 221 9.01e-14 72.4
Msa0599710 MtrunA17_Chr7g0248721 45.570 79 41 2 326 404 120 196 1.12e-13 70.5
Msa0599710 MtrunA17_Chr1g0150801 45.205 73 39 1 332 404 87 158 1.40e-13 68.6
Msa0599710 MtrunA17_Chr1g0147881 43.529 85 46 1 332 414 245 329 1.66e-13 71.6
Msa0599710 MtrunA17_Chr1g0202461 39.785 93 54 1 345 437 259 349 1.70e-13 72.0
Msa0599710 MtrunA17_Chr1g0188941 43.939 66 36 1 342 407 46 110 5.76e-13 65.5
Msa0599710 MtrunA17_Chr5g0418781 42.308 78 44 1 327 404 104 180 6.60e-13 68.9
Msa0599710 MtrunA17_Chr2g0289181 38.961 77 47 0 342 418 9 85 7.79e-13 68.2
Msa0599710 MtrunA17_Chr8g0383251 43.243 74 41 1 334 407 95 167 9.30e-13 67.0
Msa0599710 MtrunA17_Chr8g0348311 42.667 75 43 0 343 417 151 225 1.28e-12 68.9
Msa0599710 MtrunA17_Chr3g0129321 44.737 76 41 1 333 408 104 178 1.54e-12 66.6
Msa0599710 MtrunA17_Chr4g0053281 48.387 62 32 0 348 409 267 328 2.14e-12 68.2
Msa0599710 MtrunA17_Chr1g0189391 45.833 72 37 2 334 404 84 154 7.30e-12 66.6
Msa0599710 MtrunA17_Chr1g0184691 49.123 57 29 0 348 404 294 350 7.65e-12 66.6
Msa0599710 MtrunA17_Chr5g0401821 48.333 60 31 0 347 406 199 258 1.52e-11 65.1
Msa0599710 MtrunA17_Chr8g0335811 41.772 79 45 1 326 404 133 210 2.05e-11 63.9
Msa0599710 MtrunA17_Chr4g0074001 41.250 80 46 1 332 411 344 422 2.68e-11 65.9
Msa0599710 MtrunA17_Chr6g0485431 42.500 80 44 2 334 412 141 219 9.36e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0599710 AT4G04450.1 35.886 457 218 13 38 471 14 418 5.39e-69 229
Msa0599710 AT4G04450.2 38.032 376 171 10 106 471 1 324 1.07e-63 213
Msa0599710 AT1G62300.1 63.699 146 52 1 326 470 291 436 2.69e-52 186
Msa0599710 AT4G22070.4 65.541 148 50 1 326 472 276 423 4.60e-50 179
Msa0599710 AT4G22070.3 65.541 148 50 1 326 472 276 423 4.60e-50 179
Msa0599710 AT4G22070.2 65.541 148 50 1 326 472 276 423 4.60e-50 179
Msa0599710 AT4G22070.1 65.541 148 50 1 326 472 276 423 4.60e-50 179
Msa0599710 AT4G01720.1 58.333 144 54 1 332 469 224 367 1.01e-35 139
Msa0599710 AT1G68150.1 47.407 135 66 1 332 466 220 349 4.18e-34 132
Msa0599710 AT5G15130.2 60.000 90 36 0 332 421 170 259 3.97e-32 129
Msa0599710 AT5G15130.1 58.696 92 38 0 330 421 210 301 5.09e-32 129
Msa0599710 AT1G18860.1 29.067 375 195 8 125 476 48 374 4.97e-30 123
Msa0599710 AT1G69810.2 46.341 164 81 5 320 482 118 275 5.40e-29 117
Msa0599710 AT1G69810.1 46.341 164 81 5 320 482 176 333 7.10e-29 118
Msa0599710 AT1G13960.1 49.367 79 39 1 329 407 391 468 4.39e-19 90.5
Msa0599710 AT1G13960.2 49.367 79 39 1 329 407 238 315 6.64e-19 88.6
Msa0599710 AT2G03340.1 36.765 136 66 3 274 409 361 476 1.20e-18 89.0
Msa0599710 AT2G25000.3 32.667 150 99 2 262 410 40 188 1.23e-18 85.9
Msa0599710 AT2G25000.2 32.667 150 99 2 262 410 18 166 2.30e-18 84.7
Msa0599710 AT2G25000.4 32.667 150 99 2 262 410 18 166 2.30e-18 84.7
Msa0599710 AT2G25000.1 32.667 150 99 2 262 410 61 209 2.85e-18 85.1
Msa0599710 AT3G01080.2 43.023 86 48 1 322 407 319 403 9.91e-18 85.9
Msa0599710 AT4G26640.7 40.659 91 53 1 319 409 313 402 1.36e-17 85.9
Msa0599710 AT4G26640.5 40.659 91 53 1 319 409 177 266 1.40e-17 84.7
Msa0599710 AT4G26640.6 40.659 91 53 1 319 409 177 266 1.40e-17 84.7
Msa0599710 AT4G26640.4 40.659 91 53 1 319 409 177 266 1.40e-17 84.7
Msa0599710 AT4G26640.3 40.659 91 53 1 319 409 177 266 1.40e-17 84.7
Msa0599710 AT3G01080.1 43.023 86 48 1 322 407 281 365 1.51e-17 85.1
Msa0599710 AT4G26640.1 40.659 91 53 1 319 409 281 370 1.79e-17 85.5
Msa0599710 AT4G26640.2 40.659 91 53 1 319 409 353 442 1.85e-17 85.5
Msa0599710 AT2G38470.1 46.914 81 42 1 329 409 344 423 2.08e-17 85.5
Msa0599710 AT5G56270.1 46.914 81 42 1 329 409 469 548 2.16e-17 85.9
Msa0599710 AT4G31800.2 38.095 105 60 1 326 425 85 189 1.85e-16 79.3
Msa0599710 AT1G80840.1 42.308 78 43 1 333 408 130 207 2.21e-16 80.1
Msa0599710 AT4G31800.1 38.095 105 60 1 326 425 150 254 2.55e-16 80.1
Msa0599710 AT2G04880.2 38.710 93 56 1 317 409 253 344 2.89e-16 81.6
Msa0599710 AT5G41570.1 41.000 100 54 2 333 432 84 178 3.01e-16 77.0
Msa0599710 AT1G64000.1 50.000 76 37 1 329 404 96 170 3.06e-16 77.4
Msa0599710 AT2G04880.1 39.560 91 54 1 317 407 277 366 3.37e-16 81.3
Msa0599710 AT2G30250.1 43.023 86 48 1 326 411 307 391 4.67e-16 80.5
Msa0599710 AT5G46350.1 38.462 104 63 1 334 437 170 272 5.43e-16 79.3
Msa0599710 AT4G23550.1 44.872 78 43 0 342 419 135 212 6.41e-16 79.0
Msa0599710 AT1G29860.1 40.426 94 51 2 332 425 121 209 8.42e-16 78.2
Msa0599710 AT5G07100.2 38.017 121 64 3 301 411 85 204 1.42e-15 76.3
Msa0599710 AT5G07100.4 38.017 121 64 3 301 411 85 204 1.42e-15 76.3
Msa0599710 AT5G07100.5 38.017 121 64 3 301 411 85 204 1.42e-15 76.3
Msa0599710 AT5G07100.3 38.017 121 64 3 301 411 85 204 1.42e-15 76.3
Msa0599710 AT4G18170.1 47.368 76 39 1 332 407 157 231 1.50e-15 78.2
Msa0599710 AT5G07100.1 38.843 121 63 3 301 411 178 297 1.83e-15 77.8
Msa0599710 AT5G13080.1 49.296 71 35 1 334 404 54 123 1.89e-15 73.9
Msa0599710 AT2G46130.1 46.479 71 37 1 334 404 17 86 4.23e-15 71.6
Msa0599710 AT5G26170.1 47.222 72 37 1 333 404 99 169 5.87e-15 73.2
Msa0599710 AT4G26440.1 46.053 76 40 1 334 409 359 433 6.30e-15 77.8
Msa0599710 AT1G29280.1 45.714 70 38 0 338 407 65 134 6.65e-15 75.1
Msa0599710 AT2G47260.1 40.964 83 48 1 332 414 159 240 6.84e-15 76.3
Msa0599710 AT1G30650.1 43.373 83 46 1 333 414 202 284 1.44e-14 76.3
Msa0599710 AT5G49520.1 41.250 80 46 1 332 411 206 284 1.50e-14 75.9
Msa0599710 AT2G34830.1 43.373 83 46 1 333 414 200 282 1.54e-14 75.9
Msa0599710 AT3G58710.1 43.836 73 41 0 338 410 62 134 1.77e-14 73.9
Msa0599710 AT3G58710.2 43.836 73 41 0 338 410 61 133 1.78e-14 73.9
Msa0599710 AT2G34830.2 43.373 83 46 1 333 414 209 291 1.86e-14 75.9
Msa0599710 AT3G01970.1 48.611 72 36 1 333 404 51 121 2.76e-14 70.5
Msa0599710 AT5G52830.1 42.683 82 47 0 336 417 154 235 3.53e-14 74.3
Msa0599710 AT4G01250.1 44.156 77 43 0 341 417 122 198 7.42e-14 72.8
Msa0599710 AT4G30935.1 34.746 118 72 2 315 432 299 411 2.58e-13 72.4
Msa0599710 AT5G64810.1 44.681 94 48 2 314 404 74 166 3.01e-13 68.9
Msa0599710 AT5G45050.4 32.353 136 86 2 297 432 997 1126 9.24e-13 71.6
Msa0599710 AT5G45050.2 32.353 136 86 2 297 432 1108 1237 1.07e-12 71.2
Msa0599710 AT5G45050.1 32.353 136 86 2 297 432 1136 1265 1.08e-12 71.2
Msa0599710 AT3G62340.1 44.000 75 41 1 333 407 104 177 2.13e-12 68.2
Msa0599710 AT1G55600.1 42.105 76 43 1 334 409 294 368 2.46e-12 69.3
Msa0599710 AT2G30590.1 48.438 64 32 1 348 410 314 377 4.87e-12 67.8
Msa0599710 AT2G23320.1 47.458 59 31 0 348 406 241 299 7.05e-12 67.0
Msa0599710 AT1G69310.4 40.000 75 44 1 330 404 130 203 8.11e-12 66.2
Msa0599710 AT1G69310.2 40.000 75 44 1 330 404 130 203 8.11e-12 66.2
Msa0599710 AT1G69310.1 40.000 75 44 1 330 404 130 203 8.11e-12 66.2
Msa0599710 AT2G44745.1 38.462 91 54 2 316 405 113 202 1.68e-11 64.3
Msa0599710 AT4G24240.1 46.667 60 32 0 347 406 281 340 2.68e-11 65.5
Msa0599710 AT2G24570.1 49.123 57 29 0 348 404 244 300 4.97e-11 64.3
Msa0599710 AT5G28650.1 49.057 53 27 0 352 404 267 319 5.48e-11 64.3
Msa0599710 AT3G04670.1 49.057 53 27 0 352 404 267 319 8.63e-11 63.9

Find 79 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAACTGATCCTTACTTCT+AGG 0.157547 4_2:+39845595 None:intergenic
ACTGGGGCCAATTAAATTTA+TGG 0.164953 4_2:-39845911 Msa0599710:CDS
GCAGAACTCTAAGTGATTAA+AGG 0.177897 4_2:+39843892 None:intergenic
AATAGAGTTAGATTTCTTTA+AGG 0.203537 4_2:-39847743 Msa0599710:CDS
CCAAGATCCATAAATTTAAT+TGG 0.204486 4_2:+39845904 None:intergenic
TGGTTCCTTTGTGGAGTATT+TGG 0.246779 4_2:+39843931 None:intergenic
TCAAGTGTAATTGTTGTAAA+TGG 0.247776 4_2:+39843967 None:intergenic
GTATTTGATGGAAACTTTAA+AGG 0.252144 4_2:-39845961 Msa0599710:CDS
CTTTAACTTCCTTAACATTA+TGG 0.256499 4_2:+39845992 None:intergenic
CCAATTAAATTTATGGATCT+TGG 0.268064 4_2:-39845904 Msa0599710:CDS
TCTTGGATTGGCTAGTAATA+AGG 0.268615 4_2:-39845887 Msa0599710:CDS
CGAGATTGAACGAAAGAAAA+TGG 0.301328 4_2:-39846100 Msa0599710:CDS
AATAAGGGTAAGGAAGTTAA+AGG 0.305666 4_2:-39845871 Msa0599710:CDS
AAGGTTAAAGAAGTGTTTAA+TGG 0.314983 4_2:-39845748 Msa0599710:CDS
TGCTGGATTGGATACTAATA+AGG 0.317920 4_2:-39845767 Msa0599710:CDS
CATAATGTTAAGGAAGTTAA+AGG 0.325037 4_2:-39845991 Msa0599710:CDS
TACTGATACACCATCTTTGT+TGG 0.335785 4_2:-39847659 Msa0599710:CDS
CAAATCTCATCTCATGAAAC+AGG 0.365816 4_2:-39847766 Msa0599710:CDS
AACTGATCTTGGTTCCTTTG+TGG 0.369007 4_2:+39843922 None:intergenic
AAAAGGAAATTTGTTGATGC+TGG 0.371075 4_2:-39845784 Msa0599710:CDS
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4_2:+39844126 None:intergenic
AACTGAGAATGGTGGAGTAC+TGG 0.377375 4_2:-39845929 Msa0599710:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.378946 4_2:+39845277 None:intergenic
AAGGAGTTTGAAACTGATCT+TGG 0.394823 4_2:+39843911 None:intergenic
AAAGGAAAAGAAGTATTTGA+TGG 0.422383 4_2:-39845973 Msa0599710:CDS
GAAATTTGTTGATGCTGGAT+TGG 0.422880 4_2:-39845779 Msa0599710:CDS
TATTTGATGGAAACTTTAAA+GGG 0.425379 4_2:-39845960 Msa0599710:CDS
TAAATTTATGGATCTTGGAT+TGG 0.436594 4_2:-39845899 Msa0599710:CDS
GATGCAAGACCATAATGTTA+AGG 0.441157 4_2:-39846001 Msa0599710:CDS
CTTGGATTGGCTAGTAATAA+GGG 0.449698 4_2:-39845886 Msa0599710:CDS
GTTGTAAATGGAGCAGAAGT+TGG 0.460345 4_2:+39843979 None:intergenic
ACCTGCAGCAGCCAAAGAAA+TGG 0.462652 4_2:-39844127 Msa0599710:CDS
AATGAGAAAACCTAGAAGTA+AGG 0.465713 4_2:-39845605 Msa0599710:CDS
CCTAGAAGTAAGGATCAGTT+AGG 0.469639 4_2:-39845595 Msa0599710:CDS
AGTTAGTGCAAAGGCAATGT+AGG 0.470279 4_2:-39845687 Msa0599710:CDS
ACTGAGAATGGTGGAGTACT+GGG 0.475263 4_2:-39845928 Msa0599710:CDS
ATGATCATTGTCAGTGGCAG+AGG 0.475820 4_2:+39847687 None:intergenic
AGTATTGGTAGTAACATGAT+TGG 0.476430 4_2:+39847448 None:intergenic
TAGTAGTGATCAATCATTGA+TGG 0.477983 4_2:-39847426 Msa0599710:CDS
TGGTTGCTCAGTCCGAAAAC+AGG 0.478137 4_2:-39845245 Msa0599710:intron
GCATCAACATCAAGTAATGA+TGG 0.486827 4_2:-39844066 Msa0599710:CDS
TTTCATGAGATGAGATTTGA+GGG 0.487782 4_2:+39847770 None:intergenic
TAATAACATTACCTCCCTCT+TGG 0.496688 4_2:+39847366 None:intergenic
GTTTCATGAGATGAGATTTG+AGG 0.503147 4_2:+39847769 None:intergenic
TTATTGCTTTAGATCACTGA+TGG 0.505356 4_2:-39845349 Msa0599710:intron
GAGTTAATGATGACTTGGAA+AGG 0.508115 4_2:+39847811 None:intergenic
AAGCATCGAGGTTGCTTCGA+AGG 0.510775 4_2:-39845560 Msa0599710:CDS
ATGATGGAGAATTAGTGAAA+AGG 0.514995 4_2:-39845801 Msa0599710:CDS
CGATAATAAGCTTTAGGACA+CGG 0.531100 4_2:+39845283 None:intergenic
TGGAGGAGTTAATGATGACT+TGG 0.535839 4_2:+39847806 None:intergenic
ATTGGCTAGTAATAAGGGTA+AGG 0.543564 4_2:-39845881 Msa0599710:CDS
TGATTGATCACTACTAGTAT+TGG 0.547112 4_2:+39847433 None:intergenic
AGTTAATGATGACTTGGAAA+GGG 0.551831 4_2:+39847812 None:intergenic
GATCAATCATTGATGGATGA+TGG 0.555481 4_2:-39847419 Msa0599710:CDS
AGATCACTGATGGATGTCAA+TGG 0.557337 4_2:-39845339 Msa0599710:CDS
CTGCAGGTAACAAGTGGTTG+TGG 0.557747 4_2:+39844142 None:intergenic
AAAGGACAAGAAGTTTCTGA+TGG 0.557815 4_2:-39845853 Msa0599710:CDS
AGCTTGAGTTCCAACAAAGA+TGG 0.564900 4_2:+39847649 None:intergenic
ACTTAGTCCCAACGAAAACA+TGG 0.569432 4_2:-39847854 Msa0599710:CDS
TATCGCTGCAGCACATCCCA+TGG 0.572408 4_2:-39845265 Msa0599710:CDS
GATAATAAGCTTTAGGACAC+GGG 0.575823 4_2:+39845284 None:intergenic
GTATTGGTAGTAACATGATT+GGG 0.578993 4_2:+39847449 None:intergenic
GTTAGTGCAAAGGCAATGTA+GGG 0.584846 4_2:-39845686 Msa0599710:CDS
TGCGGACATAAATTGTCGAA+AGG 0.592856 4_2:-39845310 Msa0599710:CDS
TACTAATGCTGAGACTGCGA+TGG 0.595069 4_2:-39845650 Msa0599710:CDS
TGGCTGCTGCAGGTAACAAG+TGG 0.612014 4_2:+39844136 None:intergenic
TTCCAACTTAACAGGTACAA+AGG 0.616787 4_2:-39844209 Msa0599710:intron
ATGAATATGATCATTGTCAG+TGG 0.630171 4_2:+39847681 None:intergenic
AAGAAGCATCTTTGCAGCTG+CGG 0.630421 4_2:+39844092 None:intergenic
TGTTAATGTTGATAATGTTG+AGG 0.631518 4_2:-39845512 Msa0599710:CDS
AGCTAGATCAGAAGAAACCA+TGG 0.635656 4_2:-39845458 Msa0599710:intron
AGGCAGAGACAACTTCATGA+TGG 0.641320 4_2:+39847707 None:intergenic
GAATAGATTACGATTACATG+CGG 0.647686 4_2:-39846041 Msa0599710:CDS
AATCTCCAAATACTCCACAA+AGG 0.678231 4_2:-39843936 Msa0599710:CDS
TTCATGAGATGAGATTTGAG+GGG 0.681830 4_2:+39847771 None:intergenic
CTGAGAATGGTGGAGTACTG+GGG 0.687268 4_2:-39845927 Msa0599710:CDS
ATGGTGGAGAGTTAGTGCAA+AGG 0.698402 4_2:-39845696 Msa0599710:CDS
GGATGTCAATGGAGAAAGTG+CGG 0.714843 4_2:-39845328 Msa0599710:CDS
ATCGACGATGAAAAGCATCG+AGG 0.779533 4_2:-39845572 Msa0599710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGTAAAAAAAACTATAA+TGG + chr4_2:39844196-39844215 None:intergenic 10.0%
!!! TTTTTTTCTTTTCATTTTAT+TGG - chr4_2:39844489-39844508 Msa0599710:intron 10.0%
!!! TTTTTTCTTTTCATTTTATT+GGG - chr4_2:39844490-39844509 Msa0599710:intron 10.0%
!!! AACTTTATTTTTCTTTTATT+TGG - chr4_2:39845131-39845150 Msa0599710:intron 10.0%
!! TGAATAAATAAATTCAATTT+AGG + chr4_2:39845194-39845213 None:intergenic 10.0%
!!! TTGTTTTTTCAAATTATTAT+TGG - chr4_2:39845304-39845323 Msa0599710:CDS 10.0%
!!! AAAAACAATTTTTTTTTAGA+GGG + chr4_2:39846752-39846771 None:intergenic 10.0%
!!! AAAAACAAATTTTTTTTACA+GGG - chr4_2:39846984-39847003 Msa0599710:intron 10.0%
!!! ATACATATTTTGATTTTTAA+TGG + chr4_2:39847390-39847409 None:intergenic 10.0%
!! TATGGTTGAAAAAATTAAAA+TGG + chr4_2:39844644-39844663 None:intergenic 15.0%
!! GTAAGAGATAAAAAAAATAT+AGG + chr4_2:39844721-39844740 None:intergenic 15.0%
!!! ATAGAATATTTTATGTGATT+TGG - chr4_2:39845409-39845428 Msa0599710:intron 15.0%
!! AAAAAGAAAATGAATAATGA+TGG - chr4_2:39845975-39845994 Msa0599710:CDS 15.0%
!! TAAGTAAAATACATGTAATT+TGG + chr4_2:39846593-39846612 None:intergenic 15.0%
!!! TACATGTATTTTACTTATTA+AGG - chr4_2:39846596-39846615 Msa0599710:intron 15.0%
!! AAATATGATTGACTATAATA+TGG - chr4_2:39846688-39846707 Msa0599710:intron 15.0%
!!! CAAAAACAATTTTTTTTTAG+AGG + chr4_2:39846753-39846772 None:intergenic 15.0%
!!! CTCTAAAAAAAAATTGTTTT+TGG - chr4_2:39846751-39846770 Msa0599710:intron 15.0%
!!! AAAAAACAAAAAATTTTGCA+GGG + chr4_2:39846778-39846797 None:intergenic 15.0%
!!! TAAAAAACAAAAAATTTTGC+AGG + chr4_2:39846779-39846798 None:intergenic 15.0%
!!! AAAAAACAAAATATTTTGCA+GGG + chr4_2:39846880-39846899 None:intergenic 15.0%
!!! TAAAAAACAAAATATTTTGC+AGG + chr4_2:39846881-39846900 None:intergenic 15.0%
!!! AAAAACAAAAAAATTTTGCA+GGG + chr4_2:39846911-39846930 None:intergenic 15.0%
!!! CAAAAACAAATTTTTTTTAC+AGG - chr4_2:39846983-39847002 Msa0599710:intron 15.0%
!! TAATTAAAATACTTATGAAG+TGG - chr4_2:39847527-39847546 Msa0599710:intron 15.0%
!! AATAGAGTTAGATTTCTTTA+AGG - chr4_2:39844049-39844068 Msa0599710:CDS 20.0%
!!! TATAATGGACTATTGAATTA+TGG + chr4_2:39844181-39844200 None:intergenic 20.0%
!! TAATTTCATTAAATTCAGCT+AGG + chr4_2:39844455-39844474 None:intergenic 20.0%
!! TTGAAATAAAGGGAAAAAAT+GGG + chr4_2:39844800-39844819 None:intergenic 20.0%
!! TTGTATATGGTTGAAATAAA+GGG + chr4_2:39844810-39844829 None:intergenic 20.0%
!! ATTGTATATGGTTGAAATAA+AGG + chr4_2:39844811-39844830 None:intergenic 20.0%
!! GAAAACATAGATATTGTATA+TGG + chr4_2:39844823-39844842 None:intergenic 20.0%
!! GTCATATTCATTAATTAACA+CGG + chr4_2:39844914-39844933 None:intergenic 20.0%
!! TTTAAAAACACTAACTAATG+CGG - chr4_2:39844952-39844971 Msa0599710:intron 20.0%
!! TCAATTAATAACGAGTTTAA+TGG + chr4_2:39845038-39845057 None:intergenic 20.0%
!! AAACAATAATACTTAAACAC+CGG + chr4_2:39845359-39845378 None:intergenic 20.0%
!!! TTAAGTATTATTGTTTTGTC+CGG - chr4_2:39845363-39845382 Msa0599710:intron 20.0%
!! TATTTGATGGAAACTTTAAA+GGG - chr4_2:39845832-39845851 Msa0599710:CDS 20.0%
!!! AGTTAGTTTTAAGTAAGAAT+GGG - chr4_2:39846255-39846274 Msa0599710:intron 20.0%
!!! CAAAAACAAAAAAATTTTGC+AGG + chr4_2:39846912-39846931 None:intergenic 20.0%
!!! TCAAAAACAAAAAATTTTGC+AGG - chr4_2:39846957-39846976 Msa0599710:intron 20.0%
!!! CAAAAACAAAAAATTTTGCA+GGG - chr4_2:39846958-39846977 Msa0599710:intron 20.0%
!!! AAATCATATTGGCTAAAATA+TGG + chr4_2:39847048-39847067 None:intergenic 20.0%
!! AAGATAATGATTAAAGACTA+AGG + chr4_2:39847155-39847174 None:intergenic 20.0%
!! TCAAAATATGTATTGAAGTT+TGG - chr4_2:39847397-39847416 Msa0599710:CDS 20.0%
!!! TTTTTTCTTCTTCAACAGAT+TGG - chr4_2:39844319-39844338 Msa0599710:intron 25.0%
!! TTTTTGTCTTCTAAATTACG+TGG + chr4_2:39844403-39844422 None:intergenic 25.0%
! TCAACCATATATATGTTTCT+TGG - chr4_2:39844655-39844674 Msa0599710:intron 25.0%
! GTTGAAATAAAGGGAAAAAA+TGG + chr4_2:39844801-39844820 None:intergenic 25.0%
! TTAACACGGTAATATAACAT+TGG + chr4_2:39844900-39844919 None:intergenic 25.0%
! ATTAATAACGAGTTTAATGG+AGG + chr4_2:39845035-39845054 None:intergenic 25.0%
! ACTAACATATACATAAACAC+CGG + chr4_2:39845385-39845404 None:intergenic 25.0%
! TTAGAACACTATTAACATGT+TGG - chr4_2:39845504-39845523 Msa0599710:CDS 25.0%
! TAGAACACTATTAACATGTT+GGG - chr4_2:39845505-39845524 Msa0599710:CDS 25.0%
! CCAACAAAAACAAAATGTAT+TGG + chr4_2:39845533-39845552 None:intergenic 25.0%
!!! CCAATACATTTTGTTTTTGT+TGG - chr4_2:39845530-39845549 Msa0599710:CDS 25.0%
! GATAATCGAATGCAATTTAA+TGG - chr4_2:39845552-39845571 Msa0599710:CDS 25.0%
! AAACATCATCGCATAAAAAA+CGG + chr4_2:39845633-39845652 None:intergenic 25.0%
!! TTGATTGAATTGTTTTCAGA+TGG - chr4_2:39845656-39845675 Msa0599710:CDS 25.0%
!! CTTTAACTTCCTTAACATTA+TGG + chr4_2:39845803-39845822 None:intergenic 25.0%
! CATAATGTTAAGGAAGTTAA+AGG - chr4_2:39845801-39845820 Msa0599710:CDS 25.0%
!!! AAAGGAAAAGAAGTATTTGA+TGG - chr4_2:39845819-39845838 Msa0599710:CDS 25.0%
! GTATTTGATGGAAACTTTAA+AGG - chr4_2:39845831-39845850 Msa0599710:CDS 25.0%
! CCAAGATCCATAAATTTAAT+TGG + chr4_2:39845891-39845910 None:intergenic 25.0%
! CCAATTAAATTTATGGATCT+TGG - chr4_2:39845888-39845907 Msa0599710:CDS 25.0%
! TAAATTTATGGATCTTGGAT+TGG - chr4_2:39845893-39845912 Msa0599710:CDS 25.0%
!!! AAGGTTAAAGAAGTGTTTAA+TGG - chr4_2:39846044-39846063 Msa0599710:CDS 25.0%
!!! GAGTTAGTTTTAAGTAAGAA+TGG - chr4_2:39846254-39846273 Msa0599710:intron 25.0%
!!! GTTAGTTTTAAGTAAGAATG+GGG - chr4_2:39846256-39846275 Msa0599710:intron 25.0%
!!! TGTTAATGTTGATAATGTTG+AGG - chr4_2:39846280-39846299 Msa0599710:intron 25.0%
! ACAATTTATCATACTTACCA+TGG + chr4_2:39846354-39846373 None:intergenic 25.0%
! ATAAATTCTTTCAGCGATAA+AGG + chr4_2:39846421-39846440 None:intergenic 25.0%
! TAATGTGAAAGCAATCATTA+GGG + chr4_2:39846638-39846657 None:intergenic 25.0%
! ATAATGTGAAAGCAATCATT+AGG + chr4_2:39846639-39846658 None:intergenic 25.0%
! TGTTGTTGCATAAATCATAT+TGG + chr4_2:39847059-39847078 None:intergenic 25.0%
!! CATGTTTCTATTCTAGAAAA+TGG - chr4_2:39847308-39847327 Msa0599710:intron 25.0%
!! TCTATTCTAGAAAATGGATT+TGG - chr4_2:39847314-39847333 Msa0599710:intron 25.0%
! TATCTTGATATCTACCATTT+CGG + chr4_2:39847504-39847523 None:intergenic 25.0%
! TAATACAATTCCAACTTAAC+AGG - chr4_2:39847575-39847594 Msa0599710:intron 25.0%
! TCAAGTGTAATTGTTGTAAA+TGG + chr4_2:39847828-39847847 None:intergenic 25.0%
! AGTTAATGATGACTTGGAAA+GGG + chr4_2:39843983-39844002 None:intergenic 30.0%
TTTCATGAGATGAGATTTGA+GGG + chr4_2:39844025-39844044 None:intergenic 30.0%
ATGAATATGATCATTGTCAG+TGG + chr4_2:39844114-39844133 None:intergenic 30.0%
! GTATTGGTAGTAACATGATT+GGG + chr4_2:39844346-39844365 None:intergenic 30.0%
!! AGTATTGGTAGTAACATGAT+TGG + chr4_2:39844347-39844366 None:intergenic 30.0%
TGATTGATCACTACTAGTAT+TGG + chr4_2:39844362-39844381 None:intergenic 30.0%
TAGTAGTGATCAATCATTGA+TGG - chr4_2:39844366-39844385 Msa0599710:intron 30.0%
AGAAGACAAAAAAAACCAAG+AGG - chr4_2:39844411-39844430 Msa0599710:intron 30.0%
GAAGACAAAAAAAACCAAGA+GGG - chr4_2:39844412-39844431 Msa0599710:intron 30.0%
ATACTCACATAGACAACAAT+GGG - chr4_2:39844601-39844620 Msa0599710:intron 30.0%
CCAACCAAGAAACATATATA+TGG + chr4_2:39844662-39844681 None:intergenic 30.0%
CCATATATATGTTTCTTGGT+TGG - chr4_2:39844659-39844678 Msa0599710:intron 30.0%
AAATAAAGGGAAAAAATGGG+TGG + chr4_2:39844797-39844816 None:intergenic 30.0%
CTCGTTATTAATTGATGTAG+TGG - chr4_2:39845043-39845062 Msa0599710:intron 30.0%
!!! GTCAGTTTTATTGTTTTGTC+CGG - chr4_2:39845337-39845356 Msa0599710:CDS 30.0%
AGAACACTATTAACATGTTG+GGG - chr4_2:39845506-39845525 Msa0599710:CDS 30.0%
GAATAGATTACGATTACATG+CGG - chr4_2:39845751-39845770 Msa0599710:CDS 30.0%
AATAAGGGTAAGGAAGTTAA+AGG - chr4_2:39845921-39845940 Msa0599710:CDS 30.0%
! ATGATGGAGAATTAGTGAAA+AGG - chr4_2:39845991-39846010 Msa0599710:CDS 30.0%
!! AAAAGGAAATTTGTTGATGC+TGG - chr4_2:39846008-39846027 Msa0599710:CDS 30.0%
AAGAAAAAGAGAACTGAGAA+TGG - chr4_2:39846077-39846096 Msa0599710:CDS 30.0%
AATGAGAAAACCTAGAAGTA+AGG - chr4_2:39846187-39846206 Msa0599710:intron 30.0%
! TTATTGCTTTAGATCACTGA+TGG - chr4_2:39846443-39846462 Msa0599710:intron 30.0%
! AGATGAAAGTTACCTGTTTT+CGG + chr4_2:39846562-39846581 None:intergenic 30.0%
ATGCAAATCATCACAAAACT+GGG + chr4_2:39846816-39846835 None:intergenic 30.0%
TATGCAAATCATCACAAAAC+TGG + chr4_2:39846817-39846836 None:intergenic 30.0%
! TTTGTGATGATTTGCATACA+TGG - chr4_2:39846819-39846838 Msa0599710:intron 30.0%
! TGAACCCATTTTGTAGTTTT+TGG - chr4_2:39846853-39846872 Msa0599710:intron 30.0%
!!! TTGTTTTTGAAAATAGTCCC+TGG - chr4_2:39846923-39846942 Msa0599710:intron 30.0%
TAACAAACTCAAATCCGAAA+TGG - chr4_2:39847487-39847506 Msa0599710:intron 30.0%
GAAATGGTAGATATCAAGAT+AGG - chr4_2:39847503-39847522 Msa0599710:intron 30.0%
!!! AGTTGTTTTTGCCATTTCTT+TGG + chr4_2:39847679-39847698 None:intergenic 30.0%
GTTGCAATACTTGAAAAACA+AGG + chr4_2:39847789-39847808 None:intergenic 30.0%
!!! TGGGACTAAGTTTTAACTCA+AGG + chr4_2:39843929-39843948 None:intergenic 35.0%
! GAGTTAATGATGACTTGGAA+AGG + chr4_2:39843984-39844003 None:intergenic 35.0%
!! TTGAGGGGTTTGATTTTCAT+TGG + chr4_2:39844009-39844028 None:intergenic 35.0%
TTCATGAGATGAGATTTGAG+GGG + chr4_2:39844024-39844043 None:intergenic 35.0%
GTTTCATGAGATGAGATTTG+AGG + chr4_2:39844026-39844045 None:intergenic 35.0%
CAAATCTCATCTCATGAAAC+AGG - chr4_2:39844026-39844045 Msa0599710:CDS 35.0%
TACTGATACACCATCTTTGT+TGG - chr4_2:39844133-39844152 Msa0599710:CDS 35.0%
! GATCAATCATTGATGGATGA+TGG - chr4_2:39844373-39844392 Msa0599710:intron 35.0%
TAATAACATTACCTCCCTCT+TGG + chr4_2:39844429-39844448 None:intergenic 35.0%
TCGTTAGATATGAAGATGTC+CGG - chr4_2:39844547-39844566 Msa0599710:intron 35.0%
TATGAGTGTCACACATTGAT+GGG + chr4_2:39844574-39844593 None:intergenic 35.0%
CATACTCACATAGACAACAA+TGG - chr4_2:39844600-39844619 Msa0599710:intron 35.0%
TACTCACATAGACAACAATG+GGG - chr4_2:39844602-39844621 Msa0599710:intron 35.0%
! ATTGTGTGTGCAAATTTGCT+TGG - chr4_2:39844996-39845015 Msa0599710:intron 35.0%
! TTGTGTGTGCAAATTTGCTT+GGG - chr4_2:39844997-39845016 Msa0599710:intron 35.0%
ATAACGAGTTTAATGGAGGT+GGG + chr4_2:39845031-39845050 None:intergenic 35.0%
AATAACGAGTTTAATGGAGG+TGG + chr4_2:39845032-39845051 None:intergenic 35.0%
TTGATGTAGTGGCACATTAT+TGG - chr4_2:39845054-39845073 Msa0599710:intron 35.0%
! CCTCTGCTACTCAAATTTTT+CGG - chr4_2:39845229-39845248 Msa0599710:intron 35.0%
! CTCTGCTACTCAAATTTTTC+GGG - chr4_2:39845230-39845249 Msa0599710:intron 35.0%
! CATCGCATAAAAAACGGATT+TGG + chr4_2:39845627-39845646 None:intergenic 35.0%
TTTCAGATGGCTATTCTTCA+AGG - chr4_2:39845669-39845688 Msa0599710:CDS 35.0%
CGAGATTGAACGAAAGAAAA+TGG - chr4_2:39845692-39845711 Msa0599710:CDS 35.0%
GATGCAAGACCATAATGTTA+AGG - chr4_2:39845791-39845810 Msa0599710:CDS 35.0%
AAAAAGAGAACTGAGAATGG+TGG - chr4_2:39845855-39845874 Msa0599710:CDS 35.0%
ACTGGGGCCAATTAAATTTA+TGG - chr4_2:39845881-39845900 Msa0599710:CDS 35.0%
!! TCTTGGATTGGCTAGTAATA+AGG - chr4_2:39845905-39845924 Msa0599710:CDS 35.0%
!! CTTGGATTGGCTAGTAATAA+GGG - chr4_2:39845906-39845925 Msa0599710:CDS 35.0%
ATTGGCTAGTAATAAGGGTA+AGG - chr4_2:39845911-39845930 Msa0599710:CDS 35.0%
AAAGGACAAGAAGTTTCTGA+TGG - chr4_2:39845939-39845958 Msa0599710:CDS 35.0%
!! GAAATTTGTTGATGCTGGAT+TGG - chr4_2:39846013-39846032 Msa0599710:CDS 35.0%
! TGCTGGATTGGATACTAATA+AGG - chr4_2:39846025-39846044 Msa0599710:CDS 35.0%
AAAAAGAGAACTGAGAATGG+TGG - chr4_2:39845855-39845874 Msa0599710:CDS 35.0%
! TCTAGGTTTTCTCATTGATG+AGG + chr4_2:39846183-39846202 None:intergenic 35.0%
!! GATAATAAGCTTTAGGACAC+GGG + chr4_2:39846511-39846530 None:intergenic 35.0%
!! CGATAATAAGCTTTAGGACA+CGG + chr4_2:39846512-39846531 None:intergenic 35.0%
CAAAACGAGGCATATTTGTA+GGG + chr4_2:39846721-39846740 None:intergenic 35.0%
TGCAAATCATCACAAAACTG+GGG + chr4_2:39846815-39846834 None:intergenic 35.0%
GGGACCAAAAACTACAAAAT+GGG + chr4_2:39846860-39846879 None:intergenic 35.0%
AGGGACCAAAAACTACAAAA+TGG + chr4_2:39846861-39846880 None:intergenic 35.0%
!! TTTTTGAAAATAGTCCCTGG+AGG - chr4_2:39846926-39846945 Msa0599710:intron 35.0%
!! TTTTGAAAATAGTCCCTGGA+GGG - chr4_2:39846927-39846946 Msa0599710:intron 35.0%
!! TTTTGAAAATAGTCCCTCCA+GGG + chr4_2:39846943-39846962 None:intergenic 35.0%
!! TTTTTGAAAATAGTCCCTCC+AGG + chr4_2:39846944-39846963 None:intergenic 35.0%
CAAAACGAGACATATTTGCA+GGG - chr4_2:39847015-39847034 Msa0599710:intron 35.0%
TATGTTTGTGACTTCGATCT+AGG + chr4_2:39847124-39847143 None:intergenic 35.0%
TGATATGCATTGAACGATGT+TGG - chr4_2:39847241-39847260 Msa0599710:intron 35.0%
!! AGAAACATGAGCTTTGAAAG+TGG + chr4_2:39847297-39847316 None:intergenic 35.0%
! TTTAATGGACATTGACGTGT+TGG + chr4_2:39847375-39847394 None:intergenic 35.0%
ATGGTTTCTGTATCGAAGAT+TGG + chr4_2:39847428-39847447 None:intergenic 35.0%
TCACATAGTAAGTACATCGA+TGG + chr4_2:39847447-39847466 None:intergenic 35.0%
GTACTTACTATGTGAATGCA+TGG - chr4_2:39847452-39847471 Msa0599710:CDS 35.0%
TTCCAACTTAACAGGTACAA+AGG - chr4_2:39847583-39847602 Msa0599710:intron 35.0%
GCATCAACATCAAGTAATGA+TGG - chr4_2:39847726-39847745 Msa0599710:CDS 35.0%
AATCTCCAAATACTCCACAA+AGG - chr4_2:39847856-39847875 Msa0599710:CDS 35.0%
! AAGGAGTTTGAAACTGATCT+TGG + chr4_2:39847884-39847903 None:intergenic 35.0%
ACTTAGTCCCAACGAAAACA+TGG - chr4_2:39843938-39843957 Msa0599710:CDS 40.0%
! GTAGCATCCATGTTTTCGTT+GGG + chr4_2:39843948-39843967 None:intergenic 40.0%
! TGTAGCATCCATGTTTTCGT+TGG + chr4_2:39843949-39843968 None:intergenic 40.0%
TGGAGGAGTTAATGATGACT+TGG + chr4_2:39843989-39844008 None:intergenic 40.0%
!! AGGGGTTTGATTTTCATTGG+AGG + chr4_2:39844006-39844025 None:intergenic 40.0%
AGCTTGAGTTCCAACAAAGA+TGG + chr4_2:39844146-39844165 None:intergenic 40.0%
GACAAAAAAAACCAAGAGGG+AGG - chr4_2:39844415-39844434 Msa0599710:intron 40.0%
GTATGAGTGTCACACATTGA+TGG + chr4_2:39844575-39844594 None:intergenic 40.0%
CAATGTGTGACACTCATACA+AGG - chr4_2:39844576-39844595 Msa0599710:intron 40.0%
ACTCACATAGACAACAATGG+GGG - chr4_2:39844603-39844622 Msa0599710:intron 40.0%
TAAAATGGAAGTGAGAGCCT+TGG + chr4_2:39844629-39844648 None:intergenic 40.0%
CCGAAAAATTTGAGTAGCAG+AGG + chr4_2:39845232-39845251 None:intergenic 40.0%
GTGTCATATCGATGCAATGA+CGG + chr4_2:39845257-39845276 None:intergenic 40.0%
CGTGCGAATAAAACATGTCA+GGG + chr4_2:39845595-39845614 None:intergenic 40.0%
ACGTGCGAATAAAACATGTC+AGG + chr4_2:39845596-39845615 None:intergenic 40.0%
!! ATGTTTTATTCGCACGTGAG+AGG - chr4_2:39845599-39845618 Msa0599710:CDS 40.0%
GGGAAAAAGAGAACTGAGAA+TGG - chr4_2:39845852-39845871 Msa0599710:CDS 40.0%
AGTTAGTGCAAAGGCAATGT+AGG - chr4_2:39846105-39846124 Msa0599710:intron 40.0%
GTTAGTGCAAAGGCAATGTA+GGG - chr4_2:39846106-39846125 Msa0599710:intron 40.0%
CCTAACTGATCCTTACTTCT+AGG + chr4_2:39846200-39846219 None:intergenic 40.0%
CCTAGAAGTAAGGATCAGTT+AGG - chr4_2:39846197-39846216 Msa0599710:intron 40.0%
AGCTAGATCAGAAGAAACCA+TGG - chr4_2:39846334-39846353 Msa0599710:intron 40.0%
! AGATCACTGATGGATGTCAA+TGG - chr4_2:39846453-39846472 Msa0599710:intron 40.0%
TGCGGACATAAATTGTCGAA+AGG - chr4_2:39846482-39846501 Msa0599710:intron 40.0%
CTGCAGCGATAATAAGCTTT+AGG + chr4_2:39846518-39846537 None:intergenic 40.0%
CCAAAACGAGGCATATTTGT+AGG + chr4_2:39846722-39846741 None:intergenic 40.0%
! CCTACAAATATGCCTCGTTT+TGG - chr4_2:39846719-39846738 Msa0599710:intron 40.0%
! CCTGCAAATATGTCTCGTTT+TGG + chr4_2:39847017-39847036 None:intergenic 40.0%
CCAAAACGAGACATATTTGC+AGG - chr4_2:39847014-39847033 Msa0599710:intron 40.0%
! CTGTGTAAATGCATGGATTG+CGG + chr4_2:39847352-39847371 None:intergenic 40.0%
CACCTTTGTACCTGTTAAGT+TGG + chr4_2:39847588-39847607 None:intergenic 40.0%
GTTGTAAATGGAGCAGAAGT+TGG + chr4_2:39847816-39847835 None:intergenic 40.0%
! TGGTTCCTTTGTGGAGTATT+TGG + chr4_2:39847864-39847883 None:intergenic 40.0%
AACTGATCTTGGTTCCTTTG+TGG + chr4_2:39847873-39847892 None:intergenic 40.0%
AGGCAGAGACAACTTCATGA+TGG + chr4_2:39844088-39844107 None:intergenic 45.0%
ATGATCATTGTCAGTGGCAG+AGG + chr4_2:39844108-39844127 None:intergenic 45.0%
! AAAATGGGTGGCAGCTCTAA+TGG + chr4_2:39844785-39844804 None:intergenic 45.0%
! AACTGAGAATGGTGGAGTAC+TGG - chr4_2:39845863-39845882 Msa0599710:CDS 45.0%
! ACTGAGAATGGTGGAGTACT+GGG - chr4_2:39845864-39845883 Msa0599710:CDS 45.0%
! ATGGTGGAGAGTTAGTGCAA+AGG - chr4_2:39846096-39846115 Msa0599710:CDS 45.0%
TACTAATGCTGAGACTGCGA+TGG - chr4_2:39846142-39846161 Msa0599710:intron 45.0%
!! ATCGACGATGAAAAGCATCG+AGG - chr4_2:39846220-39846239 Msa0599710:intron 45.0%
GGATGTCAATGGAGAAAGTG+CGG - chr4_2:39846464-39846483 Msa0599710:intron 45.0%
! TTTTCGGACTGAGCAACCAT+GGG + chr4_2:39846546-39846565 None:intergenic 45.0%
GAGGGACTAAAACCAAAACG+AGG + chr4_2:39846734-39846753 None:intergenic 45.0%
TCATCACAAAACTGGGGTCA+GGG + chr4_2:39846809-39846828 None:intergenic 45.0%
ATCATCACAAAACTGGGGTC+AGG + chr4_2:39846810-39846829 None:intergenic 45.0%
!! GTGTTGGCTGTGTAAATGCA+TGG + chr4_2:39847359-39847378 None:intergenic 45.0%
!! AAGAAGCATCTTTGCAGCTG+CGG + chr4_2:39847703-39847722 None:intergenic 45.0%
!! ATTATTATACTTATAAATAT+AGG + chr4_2:39844754-39844773 None:intergenic 5.0%
!!! AAATATTTTGTTTTTTAAAA+AGG - chr4_2:39846884-39846903 Msa0599710:intron 5.0%
! GTGTCACACATTGATGGGTC+CGG + chr4_2:39844569-39844588 None:intergenic 50.0%
ATAGACAACAATGGGGGCCA+AGG - chr4_2:39844609-39844628 Msa0599710:intron 50.0%
!! CTGAGAATGGTGGAGTACTG+GGG - chr4_2:39845865-39845884 Msa0599710:CDS 50.0%
!! AAGCATCGAGGTTGCTTCGA+AGG - chr4_2:39846232-39846251 Msa0599710:intron 50.0%
! GTTTTCGGACTGAGCAACCA+TGG + chr4_2:39846547-39846566 None:intergenic 50.0%
TGGTTGCTCAGTCCGAAAAC+AGG - chr4_2:39846547-39846566 Msa0599710:intron 50.0%
CTGCAGGTAACAAGTGGTTG+TGG + chr4_2:39847653-39847672 None:intergenic 50.0%
ACCTGCAGCAGCCAAAGAAA+TGG - chr4_2:39847665-39847684 Msa0599710:CDS 50.0%
TATCGCTGCAGCACATCCCA+TGG - chr4_2:39846527-39846546 Msa0599710:intron 55.0%
TGGCTGCTGCAGGTAACAAG+TGG + chr4_2:39847659-39847678 None:intergenic 55.0%
GCCATTTCTTTGGCTGCTGC+AGG + chr4_2:39847669-39847688 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 39843900 39847914 39843900 ID=Msa0599710;Name=Msa0599710
chr4_2 mRNA 39843900 39847914 39843900 ID=Msa0599710-mRNA-1;Parent=Msa0599710;Name=Msa0599710-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|5|0|488
chr4_2 exon 39847645 39847914 39847645 ID=Msa0599710-mRNA-1:exon:8691;Parent=Msa0599710-mRNA-1
chr4_2 exon 39847378 39847477 39847378 ID=Msa0599710-mRNA-1:exon:8690;Parent=Msa0599710-mRNA-1
chr4_2 exon 39845459 39846123 39845459 ID=Msa0599710-mRNA-1:exon:8689;Parent=Msa0599710-mRNA-1
chr4_2 exon 39845246 39845359 39845246 ID=Msa0599710-mRNA-1:exon:8688;Parent=Msa0599710-mRNA-1
chr4_2 exon 39843900 39844217 39843900 ID=Msa0599710-mRNA-1:exon:8687;Parent=Msa0599710-mRNA-1
chr4_2 CDS 39847645 39847914 39847645 ID=Msa0599710-mRNA-1:cds;Parent=Msa0599710-mRNA-1
chr4_2 CDS 39847378 39847477 39847378 ID=Msa0599710-mRNA-1:cds;Parent=Msa0599710-mRNA-1
chr4_2 CDS 39845459 39846123 39845459 ID=Msa0599710-mRNA-1:cds;Parent=Msa0599710-mRNA-1
chr4_2 CDS 39845246 39845359 39845246 ID=Msa0599710-mRNA-1:cds;Parent=Msa0599710-mRNA-1
chr4_2 CDS 39843900 39844217 39843900 ID=Msa0599710-mRNA-1:cds;Parent=Msa0599710-mRNA-1
Gene Sequence

>Msa0599710

ATGGATTCAAATCTAGTTAATAACAACCTTGAGTTAAAACTTAGTCCCAACGAAAACATGGATGCTACACATAGTACTATCCCTTTCCAAGTCATCATTAACTCCTCCAATGAAAATCAAACCCCTCAAATCTCATCTCATGAAACAGGAATAGAGTTAGATTTCTTTAAGGACAACAACAATGACCATCATGAAGTTGTCTCTGCCTCTGCCACTGACAATGATCATATTCATACTGATACACCATCTTTGTTGGAACTCAAGCTGAGTATTGGTCCCAATCATGTTACTACCAATACTAGTAGTGATCAATCATTGATGGATGATGGCATGCCACGTAATTTAGAAGACAAAAAAAACCAAGAGGGAGGCGAGATTGAACGAAAGAAAATGGAGAACTGTAGATTAAAAATGATGTATGATCAGCTTAGAATAGATTACGATTACATGCGGATGCGTTTTGAGAAAATGATGCAAGACCATAATGTTAAGGAAGTTAAAGGAAAAGAAGTATTTGATGGAAACTTTAAAGGGAAAAAGAGAACTGAGAATGGTGGAGTACTGGGGCCAATTAAATTTATGGATCTTGGATTGGCTAGTAATAAGGGTAAGGAAGTTAAAGGACAAGAAGTTTCTGATGGAAAATTTATAGACAAAAAGAAAATGAATAATGATGGAGAATTAGTGAAAAGGAAATTTGTTGATGCTGGATTGGATACTAATAAGGTTAAAGAAGTGTTTAATGGAAAATGTGAAAAGAAAAAGAGAACTGAGAATGGTGGAGAGTTAGTGCAAAGGCAATGTAGGGATTTTGTGTTGATTACTAATGCTGAGACTGCGATGGATCATGAAGCTTCTTCCTCATCAATGAGAAAACCTAGAAGTAAGGATCAGTTAGGATCGACGATGAAAAGCATCGAGGTTGCTTCGAAGGAGTTAGTTTTAAGTAAGAATGGGGATGTTAATGTTGATAATGTTGAGGCTACATTGACAAAAGCAAGAGTTACTATTAGAGCTAGATCAGAAGAAACCATGATCACTGATGGATGTCAATGGAGAAAGTGCGGACATAAATTGTCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACATCCCATGGTTGCTCAGTCCGAAAACAGGTACAAAGGTGTGATCTTGATAGAACAGTTGTCAACACAACATATGAAGAAAACCACAACCACTTGTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCCGCAGCTGCAAAGATGCTTCTTTCAGCATCAACATCAAGTAATGATGGAAAATTGAATGAAAACTCACTCACAAGAACACTCTTTCCTTGTTTTTCAAGTATTGCAACTATACCAACTTCTGCTCCATTTACAACAATTACACTTGATTATACTCAATCTCCAAATACTCCACAAAGGAACCAAGATCAGTTTCAAACTCCTTTAATCACTTAG
Protein sequence

>Msa0599710

MDSNLVNNNLELKLSPNENMDATHSTIPFQVIINSSNENQTPQISSHETGIELDFFKDNNNDHHEVVSASATDNDHIHTDTPSLLELKLSIGPNHVTTNTSSDQSLMDDGMPRNLEDKKNQEGGEIERKKMENCRLKMMYDQLRIDYDYMRMRFEKMMQDHNVKEVKGKEVFDGNFKGKKRTENGGVLGPIKFMDLGLASNKGKEVKGQEVSDGKFIDKKKMNNDGELVKRKFVDAGLDTNKVKEVFNGKCEKKKRTENGGELVQRQCRDFVLITNAETAMDHEASSSSMRKPRSKDQLGSTMKSIEVASKELVLSKNGDVNVDNVEATLTKARVTIRARSEETMITDGCQWRKCGHKLSKGNPCPKAYYRCSTSHGCSVRKQVQRCDLDRTVVNTTYEENHNHLLPAAAKEMAKTTSAAAKMLLSASTSSNDGKLNENSLTRTLFPCFSSIATIPTSAPFTTITLDYTQSPNTPQRNQDQFQTPLIT*