AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0600180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0600180 MtrunA17_Chr4g0025061 84.738 629 78 4 1 629 1 611 0.0 1016
Msa0600180 MtrunA17_Chr4g0025051 77.153 569 122 4 32 600 7 567 0.0 814
Msa0600180 MtrunA17_Chr4g0024391 73.441 497 123 4 133 629 1 488 0.0 686
Msa0600180 MtrunA17_Chr2g0322391 45.022 462 174 13 205 606 150 591 2.14e-94 303
Msa0600180 MtrunA17_Chr2g0288871 36.813 546 234 15 61 578 68 530 1.65e-82 270
Msa0600180 MtrunA17_Chr4g0075201 57.018 228 84 6 344 565 245 464 4.07e-72 241
Msa0600180 MtrunA17_Chr3g0099641 47.479 238 95 5 346 577 231 444 2.57e-63 217
Msa0600180 MtrunA17_Chr7g0270831 46.124 258 95 7 351 602 201 420 8.90e-57 198
Msa0600180 MtrunA17_Chr2g0302391 48.529 204 90 5 351 542 196 396 2.64e-47 172
Msa0600180 MtrunA17_Chr4g0024401 69.167 120 31 2 16 135 8 121 1.88e-45 157
Msa0600180 MtrunA17_Chr7g0216891 51.613 124 54 1 323 440 170 293 3.27e-37 146
Msa0600180 MtrunA17_Chr4g0051181 37.453 267 135 6 351 592 228 487 8.80e-36 141
Msa0600180 MtrunA17_Chr4g0060271 45.550 191 73 5 327 486 198 388 1.06e-32 133
Msa0600180 MtrunA17_Chr3g0101131 44.037 109 57 2 339 444 388 495 1.04e-24 108
Msa0600180 MtrunA17_Chr5g0418191 50.515 97 43 2 336 428 286 381 1.73e-24 106
Msa0600180 MtrunA17_Chr5g0418191 36.111 108 65 3 367 474 152 255 4.74e-11 65.5
Msa0600180 MtrunA17_Chr7g0218081 48.454 97 49 1 342 438 386 481 3.22e-24 107
Msa0600180 MtrunA17_Chr4g0001361 35.099 151 74 4 343 493 139 265 2.39e-23 101
Msa0600180 MtrunA17_Chr5g0432161 48.387 93 47 1 336 428 391 482 3.67e-23 103
Msa0600180 MtrunA17_Chr4g0042831 51.852 81 38 1 348 428 260 339 6.11e-23 101
Msa0600180 MtrunA17_Chr3g0079691 54.321 81 36 1 348 428 477 556 9.63e-23 103
Msa0600180 MtrunA17_Chr3g0079691 43.750 64 34 2 367 430 281 342 3.21e-11 66.6
Msa0600180 MtrunA17_Chr3g0106491 46.316 95 50 1 333 427 332 425 1.70e-22 101
Msa0600180 MtrunA17_Chr3g0106491 40.230 87 46 3 344 430 180 260 3.47e-12 69.3
Msa0600180 MtrunA17_Chr5g0445311 34.911 169 75 4 349 514 347 483 2.91e-22 101
Msa0600180 MtrunA17_Chr7g0243891 54.321 81 36 1 348 428 356 435 5.88e-22 100
Msa0600180 MtrunA17_Chr4g0004911 53.165 79 36 1 348 426 318 395 6.73e-22 99.8
Msa0600180 MtrunA17_Chr4g0004911 40.698 86 44 3 350 433 170 250 1.75e-11 67.0
Msa0600180 MtrunA17_Chr7g0249301 53.947 76 34 1 353 428 279 353 1.09e-21 97.4
Msa0600180 MtrunA17_Chr4g0051881 41.667 108 62 1 353 460 165 271 2.78e-21 95.5
Msa0600180 MtrunA17_Chr5g0419241 66.038 53 18 0 344 396 55 107 2.82e-21 89.4
Msa0600180 MtrunA17_Chr7g0249311 50.617 81 39 1 348 428 374 453 3.24e-21 98.2
Msa0600180 MtrunA17_Chr7g0270191 45.745 94 46 2 351 444 130 218 3.61e-21 94.4
Msa0600180 MtrunA17_Chr3g0136961 41.837 98 56 1 327 423 98 195 5.28e-21 94.0
Msa0600180 MtrunA17_Chr5g0447701 51.807 83 38 2 347 428 232 313 5.45e-21 95.5
Msa0600180 MtrunA17_Chr2g0333851 50.000 78 37 1 352 427 153 230 8.76e-21 94.0
Msa0600180 MtrunA17_Chr1g0150271 50.000 66 33 0 361 426 96 161 1.27e-20 92.0
Msa0600180 MtrunA17_Chr1g0150281 44.565 92 51 0 351 442 125 216 2.41e-20 91.7
Msa0600180 MtrunA17_Chr1g0150801 52.703 74 34 1 351 424 87 159 9.45e-20 86.7
Msa0600180 MtrunA17_Chr1g0147881 56.716 67 29 0 367 433 263 329 1.29e-19 90.9
Msa0600180 MtrunA17_Chr7g0263531 42.857 98 45 2 329 426 334 420 1.53e-19 91.7
Msa0600180 MtrunA17_Chr7g0263531 44.444 72 38 2 367 438 190 259 1.31e-11 67.0
Msa0600180 MtrunA17_Chr2g0294231 47.500 80 41 1 351 430 188 266 1.99e-19 90.9
Msa0600180 MtrunA17_Chr4g0053281 56.452 62 27 0 367 428 267 328 3.96e-19 89.4
Msa0600180 MtrunA17_Chr2g0293481 31.792 173 98 5 283 436 166 337 4.66e-19 90.9
Msa0600180 MtrunA17_Chr8g0386241 36.697 109 68 1 324 432 61 168 5.34e-19 85.1
Msa0600180 MtrunA17_Chr1g0194481 44.444 90 45 2 353 442 145 229 7.82e-19 88.2
Msa0600180 MtrunA17_Chr7g0224801 52.857 70 32 1 356 425 83 151 1.27e-18 84.0
Msa0600180 MtrunA17_Chr8g0379461 53.968 63 29 0 366 428 76 138 1.50e-18 85.5
Msa0600180 MtrunA17_Chr5g0401821 56.667 60 26 0 366 425 199 258 1.71e-18 86.3
Msa0600180 MtrunA17_Chr7g0270051 42.857 91 49 1 362 452 49 136 2.31e-18 85.5
Msa0600180 MtrunA17_Chr1g0184691 57.895 57 24 0 367 423 294 350 5.18e-18 86.3
Msa0600180 MtrunA17_Chr2g0319961 46.835 79 42 0 357 435 71 149 5.23e-18 84.3
Msa0600180 MtrunA17_Chr5g0443131 47.436 78 40 1 353 430 144 220 8.99e-18 84.7
Msa0600180 MtrunA17_Chr4g0074001 35.669 157 82 4 291 430 268 422 1.25e-17 86.7
Msa0600180 MtrunA17_Chr2g0289181 45.455 77 42 0 361 437 9 85 1.31e-17 82.8
Msa0600180 MtrunA17_Chr7g0248721 45.263 95 47 3 353 447 128 217 1.73e-17 82.0
Msa0600180 MtrunA17_Chr2g0284321 51.562 64 31 0 363 426 32 95 1.95e-17 81.6
Msa0600180 MtrunA17_Chr4g0068541 47.368 76 39 1 352 427 194 268 2.76e-17 84.7
Msa0600180 MtrunA17_Chr8g0335811 48.101 79 40 1 345 423 133 210 3.76e-17 81.3
Msa0600180 MtrunA17_Chr7g0248691 48.000 75 39 0 362 436 150 224 4.77e-17 82.8
Msa0600180 MtrunA17_Chr1g0188941 50.000 66 32 1 361 426 46 110 8.99e-17 76.6
Msa0600180 MtrunA17_Chr3g0129361 43.478 92 50 2 353 444 131 220 1.05e-16 79.7
Msa0600180 MtrunA17_Chr8g0348311 46.667 75 40 0 362 436 151 225 1.79e-16 81.3
Msa0600180 MtrunA17_Chr8g0383251 44.156 77 42 1 353 429 95 170 3.15e-16 77.4
Msa0600180 MtrunA17_Chr4g0071521 53.448 58 27 0 366 423 245 302 3.33e-16 80.1
Msa0600180 MtrunA17_Chr5g0418781 47.436 78 40 1 346 423 104 180 3.43e-16 79.3
Msa0600180 MtrunA17_Chr8g0387501 44.444 72 40 0 365 436 128 199 3.59e-16 79.3
Msa0600180 MtrunA17_Chr8g0383351 39.000 100 59 2 325 423 118 216 2.75e-15 75.9
Msa0600180 MtrunA17_Chr1g0202461 40.860 93 53 1 364 456 259 349 8.54e-15 76.6
Msa0600180 MtrunA17_Chr3g0129321 45.333 75 40 1 353 427 105 178 1.31e-14 73.2
Msa0600180 MtrunA17_Chr7g0228941 42.029 69 39 1 355 423 42 109 3.16e-14 69.7
Msa0600180 MtrunA17_Chr1g0189391 47.222 72 36 2 353 423 84 154 8.99e-14 73.2
Msa0600180 MtrunA17_Chr6g0485431 46.250 80 41 2 353 431 141 219 1.21e-13 72.8
Msa0600180 MtrunA17_Chr3g0139021 42.029 69 39 1 355 423 304 371 7.39e-13 70.9
Msa0600180 MtrunA17_Chr7g0238921 50.877 57 27 1 367 423 125 180 9.93e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0600180 AT4G04450.1 40.152 528 224 16 65 577 53 503 2.82e-85 277
Msa0600180 AT4G04450.2 41.336 479 196 15 114 577 1 409 2.95e-81 264
Msa0600180 AT1G62300.1 50.562 267 109 8 345 593 291 552 1.61e-66 228
Msa0600180 AT4G22070.4 54.400 250 86 6 345 577 276 514 6.80e-61 213
Msa0600180 AT4G22070.3 54.400 250 86 6 345 577 276 514 6.80e-61 213
Msa0600180 AT4G22070.2 54.400 250 86 6 345 577 276 514 6.80e-61 213
Msa0600180 AT4G22070.1 54.400 250 86 6 345 577 276 514 6.80e-61 213
Msa0600180 AT4G01720.1 43.051 295 127 8 300 574 170 443 1.37e-46 172
Msa0600180 AT1G68150.1 61.856 97 37 0 351 447 220 316 2.39e-38 146
Msa0600180 AT5G15130.2 36.162 271 122 9 346 578 165 422 2.81e-38 149
Msa0600180 AT5G15130.1 36.162 271 122 9 346 578 207 464 3.51e-38 149
Msa0600180 AT1G69810.2 46.023 176 84 5 320 494 103 268 1.65e-36 140
Msa0600180 AT1G69810.1 46.023 176 84 5 320 494 161 326 2.07e-36 141
Msa0600180 AT1G18860.1 30.446 381 208 10 139 504 48 386 5.50e-34 137
Msa0600180 AT2G03340.1 44.828 116 54 2 313 428 371 476 2.28e-24 107
Msa0600180 AT2G03340.1 51.667 60 27 2 367 426 251 308 1.21e-11 68.2
Msa0600180 AT1G13960.2 55.696 79 34 1 348 426 238 315 4.20e-24 104
Msa0600180 AT1G13960.2 47.222 72 36 2 367 438 77 146 7.35e-12 67.8
Msa0600180 AT4G26640.5 52.500 80 37 1 349 428 188 266 4.97e-24 105
Msa0600180 AT4G26640.6 52.500 80 37 1 349 428 188 266 4.97e-24 105
Msa0600180 AT4G26640.4 52.500 80 37 1 349 428 188 266 4.97e-24 105
Msa0600180 AT4G26640.3 52.500 80 37 1 349 428 188 266 4.97e-24 105
Msa0600180 AT1G13960.1 55.696 79 34 1 348 426 391 468 5.46e-24 106
Msa0600180 AT1G13960.1 41.111 90 46 3 354 438 212 299 5.48e-12 68.9
Msa0600180 AT2G38470.1 54.321 81 36 1 348 428 344 423 1.17e-23 105
Msa0600180 AT4G26640.1 52.500 80 37 1 349 428 292 370 1.44e-23 105
Msa0600180 AT4G26640.7 52.500 80 37 1 349 428 324 402 1.47e-23 105
Msa0600180 AT4G26640.2 52.500 80 37 1 349 428 364 442 2.07e-23 105
Msa0600180 AT4G31800.2 43.810 105 54 2 345 444 85 189 2.32e-22 97.1
Msa0600180 AT4G31800.1 43.810 105 54 2 345 444 150 254 3.23e-22 98.2
Msa0600180 AT5G56270.1 53.086 81 37 1 348 428 469 548 6.07e-22 101
Msa0600180 AT2G25000.3 32.584 178 103 4 266 429 14 188 8.46e-22 95.5
Msa0600180 AT2G04880.2 45.055 91 49 1 336 426 253 342 9.17e-22 99.4
Msa0600180 AT2G04880.2 49.275 69 31 3 362 429 107 172 8.57e-12 68.2
Msa0600180 AT3G01080.1 48.837 86 43 1 341 426 281 365 1.00e-21 98.6
Msa0600180 AT3G01080.2 48.837 86 43 1 341 426 319 403 1.02e-21 99.4
Msa0600180 AT2G04880.1 45.055 91 49 1 336 426 277 366 1.26e-21 99.0
Msa0600180 AT2G04880.1 49.275 69 31 3 362 429 107 172 9.91e-12 68.2
Msa0600180 AT1G80840.1 47.436 78 39 1 352 427 130 207 3.25e-21 95.1
Msa0600180 AT1G29860.1 46.809 94 45 2 351 444 121 209 3.79e-21 94.7
Msa0600180 AT5G07100.2 48.235 85 43 1 346 430 121 204 3.98e-21 92.8
Msa0600180 AT5G07100.4 48.235 85 43 1 346 430 121 204 3.98e-21 92.8
Msa0600180 AT5G07100.5 48.235 85 43 1 346 430 121 204 3.98e-21 92.8
Msa0600180 AT5G07100.3 48.235 85 43 1 346 430 121 204 3.98e-21 92.8
Msa0600180 AT2G25000.2 34.211 152 94 3 281 429 18 166 5.93e-21 92.8
Msa0600180 AT2G25000.4 34.211 152 94 3 281 429 18 166 5.93e-21 92.8
Msa0600180 AT5G07100.1 48.235 85 43 1 346 430 214 297 7.95e-21 94.4
Msa0600180 AT2G25000.1 34.211 152 94 3 281 429 61 209 1.13e-20 92.8
Msa0600180 AT4G18170.1 44.000 100 55 1 353 452 159 257 1.26e-20 94.0
Msa0600180 AT5G41570.1 45.455 99 49 2 353 451 85 178 1.57e-20 90.1
Msa0600180 AT1G64000.1 53.247 77 35 1 348 424 96 171 1.78e-20 90.1
Msa0600180 AT5G26170.1 54.167 72 32 1 352 423 99 169 1.80e-20 89.7
Msa0600180 AT5G46350.1 54.054 74 33 1 353 426 170 242 8.41e-20 91.7
Msa0600180 AT2G30250.1 46.512 86 45 1 345 430 307 391 1.53e-19 91.7
Msa0600180 AT2G23320.1 59.322 59 24 0 367 425 241 299 1.72e-19 90.5
Msa0600180 AT5G49520.1 46.250 80 42 1 351 430 206 284 3.02e-19 90.9
Msa0600180 AT1G29280.1 50.000 70 35 0 357 426 65 134 4.63e-19 87.8
Msa0600180 AT2G30590.1 57.812 64 26 1 367 429 314 377 6.14e-19 89.7
Msa0600180 AT4G30935.1 40.000 125 71 3 334 456 299 421 6.61e-19 90.5
Msa0600180 AT5G13080.1 52.055 73 34 1 353 425 54 125 9.78e-19 83.6
Msa0600180 AT1G55600.1 48.684 76 38 1 353 428 294 368 1.41e-18 89.7
Msa0600180 AT4G26440.1 51.316 76 36 1 353 428 359 433 2.05e-18 89.7
Msa0600180 AT3G58710.1 47.945 73 38 0 357 429 62 134 2.38e-18 86.3
Msa0600180 AT3G58710.2 47.945 73 38 0 357 429 61 133 2.59e-18 85.9
Msa0600180 AT5G28650.1 60.377 53 21 0 371 423 267 319 5.83e-18 85.9
Msa0600180 AT4G24240.1 56.667 60 26 0 366 425 281 340 8.18e-18 85.9
Msa0600180 AT3G01970.1 51.389 72 34 1 352 423 51 121 1.06e-17 80.9
Msa0600180 AT3G62340.1 52.703 74 34 1 353 426 105 177 1.13e-17 84.3
Msa0600180 AT3G04670.1 57.895 57 24 0 367 423 263 319 1.51e-17 84.7
Msa0600180 AT2G24570.1 59.649 57 23 0 367 423 244 300 1.58e-17 84.7
Msa0600180 AT2G47260.1 45.783 83 44 1 351 433 159 240 1.69e-17 84.7
Msa0600180 AT2G44745.1 39.286 112 63 3 315 423 92 201 1.76e-17 82.4
Msa0600180 AT2G46130.1 46.429 84 43 2 342 424 5 87 1.98e-17 79.0
Msa0600180 AT4G01250.1 46.429 84 43 1 353 436 117 198 2.86e-17 83.6
Msa0600180 AT2G34830.1 43.011 93 46 1 348 433 190 282 3.17e-17 85.1
Msa0600180 AT5G52830.1 45.455 77 42 0 360 436 159 235 3.19e-17 84.0
Msa0600180 AT1G30650.1 43.011 93 46 1 348 433 192 284 3.50e-17 85.1
Msa0600180 AT2G34830.2 43.011 93 46 1 348 433 199 291 4.09e-17 84.7
Msa0600180 AT4G31550.1 57.895 57 24 0 367 423 247 303 4.23e-17 83.6
Msa0600180 AT4G31550.2 57.895 57 24 0 367 423 246 302 4.29e-17 83.2
Msa0600180 AT5G64810.1 45.745 94 47 2 333 423 74 166 6.35e-17 80.1
Msa0600180 AT5G45050.4 26.939 245 156 7 294 529 971 1201 1.48e-16 84.7
Msa0600180 AT5G45050.2 26.939 245 156 7 294 529 1082 1312 1.60e-16 84.3
Msa0600180 AT5G45050.1 26.939 245 156 7 294 529 1110 1340 1.74e-16 84.3
Msa0600180 AT4G23550.1 44.872 78 43 0 361 438 135 212 2.31e-16 80.9
Msa0600180 AT4G39410.1 52.113 71 33 1 353 423 210 279 5.01e-16 79.7
Msa0600180 AT1G69310.4 44.000 75 41 1 349 423 130 203 1.32e-15 78.2
Msa0600180 AT1G69310.2 44.000 75 41 1 349 423 130 203 1.32e-15 78.2
Msa0600180 AT1G69310.1 44.000 75 41 1 349 423 130 203 1.32e-15 78.2
Msa0600180 AT2G37260.2 53.846 65 29 1 361 425 262 325 2.51e-15 78.2
Msa0600180 AT2G37260.2 48.529 68 28 3 367 429 84 149 2.10e-11 66.2
Msa0600180 AT2G37260.3 53.846 65 29 1 361 425 264 327 2.58e-15 78.2
Msa0600180 AT2G37260.3 48.529 68 28 3 367 429 86 151 2.09e-11 66.2
Msa0600180 AT2G37260.1 53.846 65 29 1 361 425 344 407 3.56e-15 78.6
Msa0600180 AT2G37260.1 48.529 68 28 3 367 429 166 231 2.28e-11 67.0
Msa0600180 AT2G21900.1 42.029 69 39 1 361 429 104 171 2.94e-13 69.7
Msa0600180 AT2G46130.2 44.048 84 41 3 342 424 5 83 5.00e-13 66.2
Msa0600180 AT5G45260.1 31.847 157 87 4 296 440 1136 1284 8.11e-13 72.4
Msa0600180 AT4G31800.3 48.438 64 28 1 345 403 150 213 5.81e-12 66.6
Msa0600180 AT5G43290.1 33.824 136 81 7 292 423 40 170 5.74e-11 64.3

Find 117 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGATACACCATCTTTAT+TGG 0.104474 4_2:-41071465 Msa0600180:CDS
CCTAACTGATCTTGACTTCT+AGG 0.119265 4_2:+41069426 None:intergenic
CCAAAACTCTGAGGGATTAA+AGG 0.176833 4_2:+41068092 None:intergenic
GACAAAGCTGCAGGAATATT+TGG 0.214770 4_2:+41067915 None:intergenic
GTTCTCTTCTCTTCTGAATT+AGG 0.215204 4_2:+41071197 None:intergenic
GCTGCTATTACATCCATTAT+TGG 0.245842 4_2:-41067891 Msa0600180:CDS
TAAAGTTTAGGTCTTGTAAT+TGG 0.259816 4_2:+41067700 None:intergenic
CCTTTAATCCCTCAGAGTTT+TGG 0.262834 4_2:-41068092 Msa0600180:CDS
ACTGGGGCCAAGGAAATTTA+TGG 0.276997 4_2:-41069757 Msa0600180:CDS
ACTGTTTGAGCAGTAATATT+TGG 0.280261 4_2:+41067960 None:intergenic
GCGTTCCTTTGTGGAGTATT+TGG 0.301041 4_2:+41068131 None:intergenic
CGTAAGGGTAAGAAAATTAA+AGG 0.314843 4_2:-41069837 Msa0600180:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.318235 4_2:-41069597 Msa0600180:CDS
AGAGCTTGAACAAACGAAAA+TGG 0.333537 4_2:-41069946 Msa0600180:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.335137 4_2:-41069717 Msa0600180:CDS
AGCTGACCCTTCACAAATAT+TGG 0.335185 4_2:-41067988 Msa0600180:CDS
CCAAGGAAATTTATGGAACT+TGG 0.341755 4_2:-41069750 Msa0600180:CDS
AGAAGAAACTTACATGCTTT+CGG 0.356423 4_2:+41069063 None:intergenic
GGATGTCAATGGAGAAAGTA+TGG 0.356478 4_2:-41069158 Msa0600180:CDS
CTACTAGTATTGATAGTAAT+AGG 0.368547 4_2:+41071251 None:intergenic
AGTATCCATGGCTTCAGTTC+CGG 0.373468 4_2:-41071729 None:intergenic
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4_2:+41068326 None:intergenic
AGAGAAGAGAACTAAGAGTA+TGG 0.375742 4_2:-41071186 Msa0600180:CDS
AACTGAGAATGGTGGAGTAC+TGG 0.377375 4_2:-41069775 Msa0600180:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.381386 4_2:+41069107 None:intergenic
TCCCTTTCCTAGTCAATATA+AGG 0.384368 4_2:-41071619 Msa0600180:CDS
AACTGATTTGCGTTCCTTTG+TGG 0.385254 4_2:+41068122 None:intergenic
TTTCAGATGGCTATTCTTCA+AGG 0.389126 4_2:-41069969 Msa0600180:intron
AAAAGGAAATTTGATGATGC+TGG 0.391734 4_2:-41069630 Msa0600180:CDS
GCTGCTGATAATAATAATAA+TGG 0.393320 4_2:-41067840 Msa0600180:CDS
GGGAAAGAGTTGTTATTAAC+CGG 0.393924 4_2:+41071710 None:intergenic
TCTTTGGCTGCTGCAGGTAA+AGG 0.394516 4_2:+41068332 None:intergenic
TCAATTGTAATAGTTGGATA+TGG 0.399033 4_2:+41068167 None:intergenic
GAAATTTATGGAACTTGGAT+TGG 0.404712 4_2:-41069745 Msa0600180:CDS
ACTTGGATTGGCTACTGATA+AGG 0.406023 4_2:-41069733 Msa0600180:CDS
TAACATCCTCATTCTTACTT+AGG 0.406214 4_2:+41069361 None:intergenic
GCAGCAGCAGTGTTGTTGTT+TGG 0.408470 4_2:+41067858 None:intergenic
GCTACCATTGCTATTTCCTT+CGG 0.410299 4_2:+41067806 None:intergenic
CTTGTTTCATGAGATGAGAT+TGG 0.411363 4_2:+41071572 None:intergenic
TCAAGGTTGTTAGTATCATC+AGG 0.414586 4_2:+41071689 None:intergenic
CTTTGGCTGCTGCAGGTAAA+GGG 0.420375 4_2:+41068333 None:intergenic
CCAAGTTCCATAAATTTCCT+TGG 0.423079 4_2:+41069750 None:intergenic
GTGTAATCAATTGTAATAGT+TGG 0.425606 4_2:+41068161 None:intergenic
AACTTACATGCTTTCGGACT+GGG 0.430854 4_2:+41069069 None:intergenic
TGATGGGAAGGAACAAAATC+AGG 0.431895 4_2:-41067727 Msa0600180:CDS
AAAATGAGAATTAAGAATGA+TGG 0.434196 4_2:-41069663 Msa0600180:CDS
TGGCTGAGTATCACCAATAA+TGG 0.440113 4_2:+41067878 None:intergenic
GTATGAATATGATCATTGTC+AGG 0.443290 4_2:+41071485 None:intergenic
ATTGGCTACTGATAAGGTTA+AGG 0.444001 4_2:-41069727 Msa0600180:CDS
ATTGGCTACTGATAAGGTTA+AGG 0.459989 4_2:-41069607 Msa0600180:CDS
ACCTGCAGCAGCCAAAGAAA+TGG 0.461509 4_2:-41068327 Msa0600180:CDS
AGCTTTAGTTCCAATAAAGA+TGG 0.463299 4_2:+41071455 None:intergenic
GACCTTATATTGACTAGGAA+AGG 0.470626 4_2:+41071617 None:intergenic
ACTGAGAATGGTGGAGTACT+GGG 0.475263 4_2:-41069774 Msa0600180:CDS
CCTAGAAGTCAAGATCAGTT+AGG 0.482042 4_2:-41069426 Msa0600180:CDS
ACATGTGGCATCAACAATGA+TGG 0.482281 4_2:-41067744 Msa0600180:CDS
GAAATTTGATGATGCTGGAT+TGG 0.483257 4_2:-41069625 Msa0600180:CDS
GCATCAACATCAAGCAATGA+TGG 0.494138 4_2:-41068266 Msa0600180:CDS
GTTGGTGTCAAGGCAATGTA+AGG 0.496162 4_2:-41069517 Msa0600180:CDS
TGCTGGATTGGCTACTGATA+AGG 0.503894 4_2:-41069613 Msa0600180:CDS
GCTTTCGGACTGGGCAACCA+TGG 0.504496 4_2:+41069078 None:intergenic
ATGATGGAGAATTAATGAAA+AGG 0.504955 4_2:-41069647 Msa0600180:CDS
AAACTTACATGCTTTCGGAC+TGG 0.512195 4_2:+41069068 None:intergenic
GAGAGTGATGCAAGACCGTA+AGG 0.513027 4_2:-41069853 Msa0600180:CDS
GGTATTGCCAATATTTGTGA+AGG 0.514432 4_2:+41067981 None:intergenic
TAGTAGTGATCAATCTATGA+TGG 0.520634 4_2:-41071234 Msa0600180:CDS
CGATAATAAGCTTTAGGACA+CGG 0.531100 4_2:+41069113 None:intergenic
TCATGAAACAAGAACAGCGT+TGG 0.533323 4_2:-41071561 Msa0600180:CDS
TACTTGAAGAACAAGGAAGA+AGG 0.534673 4_2:+41068213 None:intergenic
ACCTTATATTGACTAGGAAA+GGG 0.537487 4_2:+41071618 None:intergenic
TTATTGAGTTAGATCACTGA+TGG 0.542017 4_2:-41069179 Msa0600180:intron
TGGGACTAAGTTTCAACTCA+AGG 0.543159 4_2:+41071672 None:intergenic
GTTGTAATCACAACTTATGA+AGG 0.543545 4_2:-41068368 Msa0600180:CDS
ATGGTGGAGTACTGGGGCCA+AGG 0.545172 4_2:-41069767 Msa0600180:CDS
CTTTCGGACTGGGCAACCAT+GGG 0.546480 4_2:+41069079 None:intergenic
AGGAATTTCCAAAACTCTGA+GGG 0.547821 4_2:+41068084 None:intergenic
ACTTCAGAATGGTGGAGAGT+TGG 0.548067 4_2:-41069535 Msa0600180:CDS
AGTATTGATAGTAATAGGAA+TGG 0.556477 4_2:+41071256 None:intergenic
AAAGGAAAAGAAGTGTTCAC+TGG 0.557236 4_2:-41069579 Msa0600180:CDS
GTATTGATAGTAATAGGAAT+GGG 0.563883 4_2:+41071257 None:intergenic
TATCGCTGCAGCACGACCCA+TGG 0.564450 4_2:-41069095 Msa0600180:CDS
ATAGCAGCTGACAAAGCTGC+AGG 0.574982 4_2:+41067906 None:intergenic
GAAGAGAACTAAGAGTATGG+AGG 0.575623 4_2:-41071183 Msa0600180:intron
GATAATAAGCTTTAGGACAC+GGG 0.575823 4_2:+41069114 None:intergenic
GATCAATCTATGATGGATGA+TGG 0.577380 4_2:-41071227 Msa0600180:CDS
AGATCACTGATGGATGTCAA+TGG 0.579642 4_2:-41069169 Msa0600180:CDS
AGCAACAAAATTATCACATG+TGG 0.580366 4_2:-41067759 Msa0600180:CDS
GTATTGCCAATATTTGTGAA+GGG 0.582929 4_2:+41067982 None:intergenic
CATGTGGCATCAACAATGAT+GGG 0.583795 4_2:-41067743 Msa0600180:CDS
ACTTGAAGAACAAGGAAGAA+GGG 0.585524 4_2:+41068214 None:intergenic
TACAAATGCTGATATTGTGA+TGG 0.589604 4_2:-41069481 Msa0600180:CDS
GATAGTCCTAAGTAAGAATG+AGG 0.591251 4_2:-41069367 Msa0600180:CDS
ATGATCATTGTCAGGGACAG+AGG 0.594495 4_2:+41071493 None:intergenic
TATGAATATGATCATTGTCA+GGG 0.594834 4_2:+41071486 None:intergenic
ATGGTGGAGAGTTGGTGTCA+AGG 0.595504 4_2:-41069527 Msa0600180:CDS
TGGCATCAACAATGATGGGA+AGG 0.604261 4_2:-41067739 Msa0600180:CDS
TTGAGGGATCAGAGAAGCTG+AGG 0.609486 4_2:+41068064 None:intergenic
AATATTTGGACTAGCAGCAA+TGG 0.612014 4_2:+41067929 None:intergenic
GATGTCAATGGAGAAAGTAT+GGG 0.615340 4_2:-41069157 Msa0600180:CDS
TGGAGGACCTTATATTGACT+AGG 0.616940 4_2:+41071612 None:intergenic
TTAAAACTTAACAGGTACAA+AGG 0.618245 4_2:-41068409 Msa0600180:intron
TGTTAATGTTGATAATGTTG+AGG 0.619880 4_2:-41069343 Msa0600180:CDS
AAGTCAAGATCAGTTAGGAT+CGG 0.620379 4_2:-41069421 Msa0600180:CDS
TGGCTGCTGCAGGTAAAGGG+TGG 0.624702 4_2:+41068336 None:intergenic
CAAGGTTGTTAGTATCATCA+GGG 0.625070 4_2:+41071690 None:intergenic
GTCACCGAAGGAAATAGCAA+TGG 0.626521 4_2:-41067810 Msa0600180:CDS
AATGGCAACAATGTCACCGA+AGG 0.626879 4_2:-41067822 Msa0600180:CDS
ATTAACCGGAACTGAAGCCA+TGG 0.627492 4_2:+41071724 None:intergenic
AGCTAGATCAGAAGAAACCA+TGG 0.628836 4_2:-41069289 Msa0600180:intron
GAGGAATTTCCAAAACTCTG+AGG 0.632072 4_2:+41068083 None:intergenic
TTCATGAGATGAGATTGGAG+AGG 0.650096 4_2:+41071577 None:intergenic
AGGCAGAGACAACTTCATGA+TGG 0.653739 4_2:+41071513 None:intergenic
GAATAGATTACAATTACATG+CGG 0.660089 4_2:-41069887 Msa0600180:CDS
AATCTCCAAATACTCCACAA+AGG 0.678231 4_2:-41068136 Msa0600180:CDS
CTGAGAATGGTGGAGTACTG+GGG 0.687268 4_2:-41069773 Msa0600180:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4_2:+41068206 None:intergenic
AGAGTGATGCAAGACCGTAA+GGG 0.707223 4_2:-41069852 Msa0600180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATGATTCT+TGG - chr4_2:41068566-41068585 Msa0600180:intron 10.0%
!! TATAACATAATTATTATTTG+TGG + chr4_2:41068709-41068728 None:intergenic 10.0%
!!! TTATTTTCTCAAATTATTAT+TGG - chr4_2:41069099-41069118 Msa0600180:CDS 10.0%
!! AAATATAATCACATAAAAAA+CGG + chr4_2:41069394-41069413 None:intergenic 10.0%
!!! ATTAATTCTTTTTAGTAAAA+AGG + chr4_2:41070198-41070217 None:intergenic 10.0%
!! CGTAAAAAAATAATAACAAT+GGG + chr4_2:41067996-41068015 None:intergenic 15.0%
!! ACGTAAAAAAATAATAACAA+TGG + chr4_2:41067997-41068016 None:intergenic 15.0%
!!! TTTCTACTATGTTATTTTTT+TGG - chr4_2:41068462-41068481 Msa0600180:intron 15.0%
!! TGTATTATACTTATAAATGT+AGG + chr4_2:41068633-41068652 None:intergenic 15.0%
!! TTTAGTAAATACATGTAATT+TGG + chr4_2:41070370-41070389 None:intergenic 15.0%
!! TAATTAAATTACTTATGAAG+TGG - chr4_2:41070938-41070957 Msa0600180:intron 15.0%
!! TAATACAATTAAAACTTAAC+AGG - chr4_2:41070982-41071001 Msa0600180:intron 15.0%
!! AGCAAAAAAAATTAAAGTTT+AGG + chr4_2:41071714-41071733 None:intergenic 15.0%
!! TAATTTCATTAAATTCTGCT+AGG + chr4_2:41068262-41068281 None:intergenic 20.0%
!!! TTTTGACTCAATAGAATTAA+AGG + chr4_2:41068307-41068326 None:intergenic 20.0%
!!! AAAAATTTTAAAAGAAAGCG+AGG + chr4_2:41068445-41068464 None:intergenic 20.0%
!!! ACTATGTTATTTTTTTGGAA+AGG - chr4_2:41068467-41068486 Msa0600180:intron 20.0%
!! ATATATATATGATTCTTGGT+TGG - chr4_2:41068570-41068589 Msa0600180:intron 20.0%
!! CACTAACATACATATAAAAT+CGG + chr4_2:41069154-41069173 None:intergenic 20.0%
!! AAAATGAGAATTAAGAATGA+TGG - chr4_2:41069736-41069755 Msa0600180:CDS 20.0%
!!! ACATGCATATTTTAAAACAT+TGG - chr4_2:41070545-41070564 Msa0600180:intron 20.0%
!! ACTAAAAATGTTTATGTATG+TGG + chr4_2:41070620-41070639 None:intergenic 20.0%
!!! AATTTTGATTGGTTTTGATT+AGG + chr4_2:41071364-41071383 None:intergenic 20.0%
! TATGAATATGATCATTGTCA+GGG + chr4_2:41067916-41067935 None:intergenic 25.0%
! AACAATGGGATATTGAATTA+TGG + chr4_2:41067982-41068001 None:intergenic 25.0%
!! GTATTGATAGTAATAGGAAT+GGG + chr4_2:41068145-41068164 None:intergenic 25.0%
!!! AGTATTGATAGTAATAGGAA+TGG + chr4_2:41068146-41068165 None:intergenic 25.0%
!!! CTACTAGTATTGATAGTAAT+AGG + chr4_2:41068151-41068170 None:intergenic 25.0%
! TAATTCTATTGAGTCAAAAC+TGG - chr4_2:41068308-41068327 Msa0600180:CDS 25.0%
! AATTCTATTGAGTCAAAACT+GGG - chr4_2:41068309-41068328 Msa0600180:CDS 25.0%
! GTCATATTCATTAATTAACG+TGG + chr4_2:41068801-41068820 None:intergenic 25.0%
! ATTTAATTAGCGTACTATGT+CGG + chr4_2:41068897-41068916 None:intergenic 25.0%
! AAAATGTAACAGTTTATGTC+AGG + chr4_2:41069073-41069092 None:intergenic 25.0%
!!! GTAATTTTTTCACAAAGAAG+AGG + chr4_2:41069204-41069223 None:intergenic 25.0%
!! CATGTTAATAGTGTTCTAAA+AGG + chr4_2:41069264-41069283 None:intergenic 25.0%
! TTAGAACACTATTAACATGA+TGG - chr4_2:41069265-41069284 Msa0600180:intron 25.0%
! TAGAACACTATTAACATGAT+GGG - chr4_2:41069266-41069285 Msa0600180:intron 25.0%
! CCAACAAAATCAAAATACAT+TGG + chr4_2:41069294-41069313 None:intergenic 25.0%
!!! CCAATGTATTTTGATTTTGT+TGG - chr4_2:41069291-41069310 Msa0600180:CDS 25.0%
!! AATCACATAAAAAACGGATT+TGG + chr4_2:41069388-41069407 None:intergenic 25.0%
!! TTCATTGAACTATTTTCAGA+TGG - chr4_2:41069417-41069436 Msa0600180:CDS 25.0%
! GAATAGATTACAATTACATG+CGG - chr4_2:41069512-41069531 Msa0600180:CDS 25.0%
!!! CTTTAATTTTCTTACCCTTA+CGG + chr4_2:41069564-41069583 None:intergenic 25.0%
! AAAGGAAAAAAAGTCTTTGA+TGG - chr4_2:41069580-41069599 Msa0600180:CDS 25.0%
!! ATGATGGAGAATTAATGAAA+AGG - chr4_2:41069752-41069771 Msa0600180:CDS 25.0%
!!! TGTTAATGTTGATAATGTTG+AGG - chr4_2:41070056-41070075 Msa0600180:intron 25.0%
! TAAATACATAGATTGCTGAA+TGG + chr4_2:41070758-41070777 None:intergenic 25.0%
!!! CTGCATATTTTGATTTACAA+TGG + chr4_2:41070801-41070820 None:intergenic 25.0%
! TCAAAATATGCAGTTATGTT+TGG - chr4_2:41070808-41070827 Msa0600180:intron 25.0%
! CAATAAACTCAAATCCAAAA+TGG - chr4_2:41070898-41070917 Msa0600180:intron 25.0%
! TTAAAACTTAACAGGTACAA+AGG - chr4_2:41070990-41071009 Msa0600180:intron 25.0%
! TCAATTGTAATAGTTGGATA+TGG + chr4_2:41071235-41071254 None:intergenic 25.0%
! GTGTAATCAATTGTAATAGT+TGG + chr4_2:41071241-41071260 None:intergenic 25.0%
!! CAAAACCAATCAAAATTTTC+TGG - chr4_2:41071367-41071386 Msa0600180:intron 25.0%
! GCTGCTGATAATAATAATAA+TGG - chr4_2:41071559-41071578 Msa0600180:CDS 25.0%
!! TGTGATAATTTTGTTGCTAT+TGG + chr4_2:41071638-41071657 None:intergenic 25.0%
!! TAAAGTTTAGGTCTTGTAAT+TGG + chr4_2:41071702-41071721 None:intergenic 25.0%
! ACCTTATATTGACTAGGAAA+GGG + chr4_2:41067784-41067803 None:intergenic 30.0%
GTATGAATATGATCATTGTC+AGG + chr4_2:41067917-41067936 None:intergenic 30.0%
TACTGATACACCATCTTTAT+TGG - chr4_2:41067934-41067953 Msa0600180:CDS 30.0%
! AGCTTTAGTTCCAATAAAGA+TGG + chr4_2:41067947-41067966 None:intergenic 30.0%
TAGTAGTGATCAATCTATGA+TGG - chr4_2:41068165-41068184 Msa0600180:CDS 30.0%
! TATATGATTCTTGGTTGGTT+TGG - chr4_2:41068575-41068594 Msa0600180:intron 30.0%
!! CCTGAAAATTTGAGTATTGA+GGG + chr4_2:41069027-41069046 None:intergenic 30.0%
! CCTCAATACTCAAATTTTCA+GGG - chr4_2:41069025-41069044 Msa0600180:intron 30.0%
AGTTTATGTCAGGATCATAT+TGG + chr4_2:41069063-41069082 None:intergenic 30.0%
AGAACACTATTAACATGATG+GGG - chr4_2:41069267-41069286 Msa0600180:intron 30.0%
CGTTCGAATAAAACATGTTA+GGG + chr4_2:41069356-41069375 None:intergenic 30.0%
ACGTTCGAATAAAACATGTT+AGG + chr4_2:41069357-41069376 None:intergenic 30.0%
CGTAAGGGTAAGAAAATTAA+AGG - chr4_2:41069562-41069581 Msa0600180:CDS 30.0%
GAAATTTATGGAACTTGGAT+TGG - chr4_2:41069654-41069673 Msa0600180:CDS 30.0%
GATAAGGTTAAGGAAGTTAA+AGG - chr4_2:41069682-41069701 Msa0600180:CDS 30.0%
!! AAAAGGAAATTTGATGATGC+TGG - chr4_2:41069769-41069788 Msa0600180:CDS 30.0%
GATAAGGTTAAGGAAGTTAA+AGG - chr4_2:41069682-41069701 Msa0600180:CDS 30.0%
AAGAAAAAGAGACTTCAGAA+TGG - chr4_2:41069853-41069872 Msa0600180:CDS 30.0%
TACAAATGCTGATATTGTGA+TGG - chr4_2:41069918-41069937 Msa0600180:CDS 30.0%
TAACATCCTCATTCTTACTT+AGG + chr4_2:41070041-41070060 None:intergenic 30.0%
! ACACTTTTACATACTTACCA+TGG + chr4_2:41070130-41070149 None:intergenic 30.0%
TTATTGAGTTAGATCACTGA+TGG - chr4_2:41070220-41070239 Msa0600180:intron 30.0%
AGAAGAAACTTACATGCTTT+CGG + chr4_2:41070339-41070358 None:intergenic 30.0%
TAATGCGAAAGCAATCAATA+GGG + chr4_2:41070410-41070429 None:intergenic 30.0%
ATAATGCGAAAGCAATCAAT+AGG + chr4_2:41070411-41070430 None:intergenic 30.0%
TATGCTTCTATGCTAGAAAA+TGG - chr4_2:41070719-41070738 Msa0600180:intron 30.0%
GTTGTAATCACAACTTATGA+AGG - chr4_2:41071031-41071050 Msa0600180:intron 30.0%
!!! AGTTGTTTTTGCCATTTCTT+TGG + chr4_2:41071086-41071105 None:intergenic 30.0%
!!! GGTTTTGATTAGGTATTTGA+GGG + chr4_2:41071354-41071373 None:intergenic 30.0%
!!! TGGTTTTGATTAGGTATTTG+AGG + chr4_2:41071355-41071374 None:intergenic 30.0%
!! GTAGTCCAGAAAATTTTGAT+TGG + chr4_2:41071375-41071394 None:intergenic 30.0%
GTATTGCCAATATTTGTGAA+GGG + chr4_2:41071420-41071439 None:intergenic 30.0%
ACTGTTTGAGCAGTAATATT+TGG + chr4_2:41071442-41071461 None:intergenic 30.0%
AGCAACAAAATTATCACATG+TGG - chr4_2:41071640-41071659 Msa0600180:CDS 30.0%
! GGGAAAGAGTTGTTATTAAC+CGG + chr4_2:41067692-41067711 None:intergenic 35.0%
CAAGGTTGTTAGTATCATCA+GGG + chr4_2:41067712-41067731 None:intergenic 35.0%
TCAAGGTTGTTAGTATCATC+AGG + chr4_2:41067713-41067732 None:intergenic 35.0%
ACTTAGTCCCAACAAAAACA+TGG - chr4_2:41067739-41067758 Msa0600180:CDS 35.0%
!! GTAGCATCCATGTTTTTGTT+GGG + chr4_2:41067749-41067768 None:intergenic 35.0%
!! TGTAGCATCCATGTTTTTGT+TGG + chr4_2:41067750-41067769 None:intergenic 35.0%
TCCCTTTCCTAGTCAATATA+AGG - chr4_2:41067780-41067799 Msa0600180:CDS 35.0%
! GACCTTATATTGACTAGGAA+AGG + chr4_2:41067785-41067804 None:intergenic 35.0%
!! AGAGGTTTGATTTTCATTGG+AGG + chr4_2:41067807-41067826 None:intergenic 35.0%
!! TGGAGAGGTTTGATTTTCAT+TGG + chr4_2:41067810-41067829 None:intergenic 35.0%
CTTGTTTCATGAGATGAGAT+TGG + chr4_2:41067830-41067849 None:intergenic 35.0%
!!! TTCTTTTTTCGCATACAGCT+TGG - chr4_2:41068118-41068137 Msa0600180:CDS 35.0%
!! GATCAATCTATGATGGATGA+TGG - chr4_2:41068172-41068191 Msa0600180:CDS 35.0%
GTTCTCTTCTCTTCTGAATT+AGG + chr4_2:41068205-41068224 None:intergenic 35.0%
AGAGAAGAGAACTAAGAGTA+TGG - chr4_2:41068213-41068232 Msa0600180:CDS 35.0%
TCGACACTCTTTAGATTTGA+AGG - chr4_2:41068349-41068368 Msa0600180:CDS 35.0%
!! TCTTTAGATTTGAAGGTGTC+TGG - chr4_2:41068356-41068375 Msa0600180:CDS 35.0%
ACCCACGTACACAAAAATAT+GGG - chr4_2:41068412-41068431 Msa0600180:intron 35.0%
! CCCTCAATACTCAAATTTTC+AGG - chr4_2:41069024-41069043 Msa0600180:intron 35.0%
! CCCTGAAAATTTGAGTATTG+AGG + chr4_2:41069028-41069047 None:intergenic 35.0%
GATCTCACTATTACAATGCA+TGG - chr4_2:41069238-41069257 Msa0600180:intron 35.0%
!! ATGTTTTATTCGAACGTGAG+AGG - chr4_2:41069360-41069379 Msa0600180:CDS 35.0%
TTTCAGATGGCTATTCTTCA+AGG - chr4_2:41069430-41069449 Msa0600180:CDS 35.0%
AGAGCTTGAACAAACGAAAA+TGG - chr4_2:41069453-41069472 Msa0600180:CDS 35.0%
GAGAAAAAGAGAACTGAGAA+TGG - chr4_2:41069613-41069632 Msa0600180:CDS 35.0%
AAAAAGAGAACTGAGAATGG+TGG - chr4_2:41069616-41069635 Msa0600180:CDS 35.0%
CCAAGTTCCATAAATTTCCT+TGG + chr4_2:41069652-41069671 None:intergenic 35.0%
CCAAGGAAATTTATGGAACT+TGG - chr4_2:41069649-41069668 Msa0600180:CDS 35.0%
ATTGGCTACTGATAAGGTTA+AGG - chr4_2:41069672-41069691 Msa0600180:CDS 35.0%
!! GAAATTTGATGATGCTGGAT+TGG - chr4_2:41069774-41069793 Msa0600180:CDS 35.0%
ATTGGCTACTGATAAGGTTA+AGG - chr4_2:41069672-41069691 Msa0600180:CDS 35.0%
!! AAAGGAAAAGAAGTGTTCAC+TGG - chr4_2:41069820-41069839 Msa0600180:CDS 35.0%
AAAAAGAGACTTCAGAATGG+TGG - chr4_2:41069856-41069875 Msa0600180:CDS 35.0%
AAGTCAAGATCAGTTAGGAT+CGG - chr4_2:41069978-41069997 Msa0600180:intron 35.0%
GATAGTCCTAAGTAAGAATG+AGG - chr4_2:41070032-41070051 Msa0600180:intron 35.0%
! GATGTCAATGGAGAAAGTAT+GGG - chr4_2:41070242-41070261 Msa0600180:intron 35.0%
TATGGGCAAAAACTATCGAA+AGG - chr4_2:41070259-41070278 Msa0600180:intron 35.0%
!! GATAATAAGCTTTAGGACAC+GGG + chr4_2:41070288-41070307 None:intergenic 35.0%
!! CGATAATAAGCTTTAGGACA+CGG + chr4_2:41070289-41070308 None:intergenic 35.0%
CATGTTTGTGACTATGATCT+AGG + chr4_2:41070530-41070549 None:intergenic 35.0%
!! AGAAGCATAAGCTTTGAAAG+TGG + chr4_2:41070708-41070727 None:intergenic 35.0%
TCACGTAATAAGCACATTGA+TGG + chr4_2:41070858-41070877 None:intergenic 35.0%
TTATTGCATTCAGCAAATCC+AGG + chr4_2:41070884-41070903 None:intergenic 35.0%
! TAGCTAAGTAGCTACCATTT+TGG + chr4_2:41070915-41070934 None:intergenic 35.0%
AAAATGGTAGCTACTTAGCT+AGG - chr4_2:41070914-41070933 Msa0600180:intron 35.0%
ACTTGAAGAACAAGGAAGAA+GGG + chr4_2:41071188-41071207 None:intergenic 35.0%
TACTTGAAGAACAAGGAAGA+AGG + chr4_2:41071189-41071208 None:intergenic 35.0%
GTTGCAATACTTGAAGAACA+AGG + chr4_2:41071196-41071215 None:intergenic 35.0%
AATCTCCAAATACTCCACAA+AGG - chr4_2:41071263-41071282 Msa0600180:CDS 35.0%
AGGAATTTCCAAAACTCTGA+GGG + chr4_2:41071318-41071337 None:intergenic 35.0%
GGTATTGCCAATATTTGTGA+AGG + chr4_2:41071421-41071440 None:intergenic 35.0%
!! AATATTTGGACTAGCAGCAA+TGG + chr4_2:41071473-41071492 None:intergenic 35.0%
GCTGCTATTACATCCATTAT+TGG - chr4_2:41071508-41071527 Msa0600180:CDS 35.0%
TGGGACTAAGTTTCAACTCA+AGG + chr4_2:41067730-41067749 None:intergenic 40.0%
! TGGAGGACCTTATATTGACT+AGG + chr4_2:41067790-41067809 None:intergenic 40.0%
TTCATGAGATGAGATTGGAG+AGG + chr4_2:41067825-41067844 None:intergenic 40.0%
TCATGAAACAAGAACAGCGT+TGG - chr4_2:41067838-41067857 Msa0600180:CDS 40.0%
! GAAGAGAACTAAGAGTATGG+AGG - chr4_2:41068216-41068235 Msa0600180:CDS 40.0%
GACCCACGTACACAAAAATA+TGG - chr4_2:41068411-41068430 Msa0600180:intron 40.0%
!! GCCCATATTTTTGTGTACGT+GGG + chr4_2:41068416-41068435 None:intergenic 40.0%
GTACACAAAAATATGGGCCA+AGG - chr4_2:41068418-41068437 Msa0600180:intron 40.0%
TTAAAAGAAAGCGAGGACCT+TGG + chr4_2:41068438-41068457 None:intergenic 40.0%
TTAACGTGGCAGCATATCAT+TGG + chr4_2:41068787-41068806 None:intergenic 40.0%
GGATCATATTGGTGCAATGA+CGG + chr4_2:41069052-41069071 None:intergenic 40.0%
!! ACTTGGATTGGCTACTGATA+AGG - chr4_2:41069666-41069685 Msa0600180:CDS 40.0%
! TCTAGGTTTTCTCATTGACG+AGG + chr4_2:41069959-41069978 None:intergenic 40.0%
CCTAACTGATCTTGACTTCT+AGG + chr4_2:41069976-41069995 None:intergenic 40.0%
CCTAGAAGTCAAGATCAGTT+AGG - chr4_2:41069973-41069992 Msa0600180:intron 40.0%
AGCTAGATCAGAAGAAACCA+TGG - chr4_2:41070110-41070129 Msa0600180:intron 40.0%
! AGATCACTGATGGATGTCAA+TGG - chr4_2:41070230-41070249 Msa0600180:intron 40.0%
GGATGTCAATGGAGAAAGTA+TGG - chr4_2:41070241-41070260 Msa0600180:intron 40.0%
CTGCAGCGATAATAAGCTTT+AGG + chr4_2:41070295-41070314 None:intergenic 40.0%
AACTTACATGCTTTCGGACT+GGG + chr4_2:41070333-41070352 None:intergenic 40.0%
AAACTTACATGCTTTCGGAC+TGG + chr4_2:41070334-41070353 None:intergenic 40.0%
GATTTGATATGCCTTGCACA+TGG - chr4_2:41070648-41070667 Msa0600180:intron 40.0%
TTGTTATCCAACCATGTGCA+AGG + chr4_2:41070662-41070681 None:intergenic 40.0%
ATGGTCGTTGTATCGAAGAT+TGG + chr4_2:41070839-41070858 None:intergenic 40.0%
GCATCAACATCAAGCAATGA+TGG - chr4_2:41071133-41071152 Msa0600180:intron 40.0%
!! AACTGATTTGCGTTCCTTTG+TGG + chr4_2:41071280-41071299 None:intergenic 40.0%
! CCAAAACTCTGAGGGATTAA+AGG + chr4_2:41071310-41071329 None:intergenic 40.0%
! CCTTTAATCCCTCAGAGTTT+TGG - chr4_2:41071307-41071326 Msa0600180:intron 40.0%
GAGGAATTTCCAAAACTCTG+AGG + chr4_2:41071319-41071338 None:intergenic 40.0%
AGCTGACCCTTCACAAATAT+TGG - chr4_2:41071411-41071430 Msa0600180:intron 40.0%
GACAAAGCTGCAGGAATATT+TGG + chr4_2:41071487-41071506 None:intergenic 40.0%
TGGCTGAGTATCACCAATAA+TGG + chr4_2:41071524-41071543 None:intergenic 40.0%
!! GCTACCATTGCTATTTCCTT+CGG + chr4_2:41071596-41071615 None:intergenic 40.0%
ACATGTGGCATCAACAATGA+TGG - chr4_2:41071655-41071674 Msa0600180:CDS 40.0%
CATGTGGCATCAACAATGAT+GGG - chr4_2:41071656-41071675 Msa0600180:CDS 40.0%
TGATGGGAAGGAACAAAATC+AGG - chr4_2:41071672-41071691 Msa0600180:CDS 40.0%
AGGCAGAGACAACTTCATGA+TGG + chr4_2:41067889-41067908 None:intergenic 45.0%
ATGATCATTGTCAGGGACAG+AGG + chr4_2:41067909-41067928 None:intergenic 45.0%
! TATGGGTGTCACACATTGAC+GGG + chr4_2:41068383-41068402 None:intergenic 45.0%
! GTATGGGTGTCACACATTGA+CGG + chr4_2:41068384-41068403 None:intergenic 45.0%
TCAATGTGTGACACCCATAC+AGG - chr4_2:41068384-41068403 Msa0600180:CDS 45.0%
CAATGTGTGACACCCATACA+GGG - chr4_2:41068385-41068404 Msa0600180:CDS 45.0%
!! GGCCCATATTTTTGTGTACG+TGG + chr4_2:41068417-41068436 None:intergenic 45.0%
GCAGAAATGCATGACTCTCA+TGG + chr4_2:41068674-41068693 None:intergenic 45.0%
AGAGTGATGCAAGACCGTAA+GGG - chr4_2:41069547-41069566 Msa0600180:CDS 45.0%
! AACTGAGAATGGTGGAGTAC+TGG - chr4_2:41069624-41069643 Msa0600180:CDS 45.0%
! ACTGAGAATGGTGGAGTACT+GGG - chr4_2:41069625-41069644 Msa0600180:CDS 45.0%
ACTGGGGCCAAGGAAATTTA+TGG - chr4_2:41069642-41069661 Msa0600180:CDS 45.0%
!! TGCTGGATTGGCTACTGATA+AGG - chr4_2:41069786-41069805 Msa0600180:CDS 45.0%
! ACTTCAGAATGGTGGAGAGT+TGG - chr4_2:41069864-41069883 Msa0600180:CDS 45.0%
!! GTTGGTGTCAAGGCAATGTA+AGG - chr4_2:41069882-41069901 Msa0600180:CDS 45.0%
TGATATGCCTTGCACATGGT+TGG - chr4_2:41070652-41070671 Msa0600180:intron 45.0%
TTACGCACCTAACACATCGA+TGG - chr4_2:41070775-41070794 Msa0600180:intron 45.0%
ACAATGGCCATCGATGTGTT+AGG + chr4_2:41070785-41070804 None:intergenic 45.0%
GTGCTTATTACGTGAATGCC+TGG - chr4_2:41070863-41070882 Msa0600180:intron 45.0%
! GCGTTCCTTTGTGGAGTATT+TGG + chr4_2:41071271-41071290 None:intergenic 45.0%
AATGGCAACAATGTCACCGA+AGG - chr4_2:41071577-41071596 Msa0600180:CDS 45.0%
GTCACCGAAGGAAATAGCAA+TGG - chr4_2:41071589-41071608 Msa0600180:CDS 45.0%
! TGGCATCAACAATGATGGGA+AGG - chr4_2:41071660-41071679 Msa0600180:CDS 45.0%
!!! TTTTATATTTTTCTTTTATT+TGG - chr4_2:41068926-41068945 Msa0600180:intron 5.0%
GAGAGTGATGCAAGACCGTA+AGG - chr4_2:41069546-41069565 Msa0600180:CDS 50.0%
!! CTGAGAATGGTGGAGTACTG+GGG - chr4_2:41069626-41069645 Msa0600180:CDS 50.0%
!! ATGGTGGAGAGTTGGTGTCA+AGG - chr4_2:41069872-41069891 Msa0600180:CDS 50.0%
CTTTGGCTGCTGCAGGTAAA+GGG + chr4_2:41071069-41071088 None:intergenic 50.0%
TCTTTGGCTGCTGCAGGTAA+AGG + chr4_2:41071070-41071089 None:intergenic 50.0%
ACCTGCAGCAGCCAAAGAAA+TGG - chr4_2:41071072-41071091 Msa0600180:intron 50.0%
TTGAGGGATCAGAGAAGCTG+AGG + chr4_2:41071338-41071357 None:intergenic 50.0%
ATAGCAGCTGACAAAGCTGC+AGG + chr4_2:41071496-41071515 None:intergenic 50.0%
! GCAGCAGCAGTGTTGTTGTT+TGG + chr4_2:41071544-41071563 None:intergenic 50.0%
ACGTGGGTCTGTCCCTGTAT+GGG + chr4_2:41068400-41068419 None:intergenic 55.0%
TACGTGGGTCTGTCCCTGTA+TGG + chr4_2:41068401-41068420 None:intergenic 55.0%
! CTTTCGGACTGGGCAACCAT+GGG + chr4_2:41070323-41070342 None:intergenic 55.0%
GCCATTTCTTTGGCTGCTGC+AGG + chr4_2:41071076-41071095 None:intergenic 55.0%
!! ATGGTGGAGTACTGGGGCCA+AGG - chr4_2:41069632-41069651 Msa0600180:CDS 60.0%
TATCGCTGCAGCACGACCCA+TGG - chr4_2:41070304-41070323 Msa0600180:intron 60.0%
! GCTTTCGGACTGGGCAACCA+TGG + chr4_2:41070324-41070343 None:intergenic 60.0%
TGGCTGCTGCAGGTAAAGGG+TGG + chr4_2:41071066-41071085 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 41067677 41071744 41067677 ID=Msa0600180;Name=Msa0600180
chr4_2 mRNA 41067677 41071744 41067677 ID=Msa0600180-mRNA-1;Parent=Msa0600180;Name=Msa0600180-mRNA-1;_AED=0.02;_eAED=0.03;_QI=0|0|0|1|1|1|5|0|648
chr4_2 exon 41071451 41071744 41071451 ID=Msa0600180-mRNA-1:exon:8938;Parent=Msa0600180-mRNA-1
chr4_2 exon 41071184 41071285 41071184 ID=Msa0600180-mRNA-1:exon:8937;Parent=Msa0600180-mRNA-1
chr4_2 exon 41069290 41069985 41069290 ID=Msa0600180-mRNA-1:exon:8936;Parent=Msa0600180-mRNA-1
chr4_2 exon 41069076 41069189 41069076 ID=Msa0600180-mRNA-1:exon:8935;Parent=Msa0600180-mRNA-1
chr4_2 exon 41067677 41068417 41067677 ID=Msa0600180-mRNA-1:exon:8934;Parent=Msa0600180-mRNA-1
chr4_2 CDS 41071451 41071744 41071451 ID=Msa0600180-mRNA-1:cds;Parent=Msa0600180-mRNA-1
chr4_2 CDS 41071184 41071285 41071184 ID=Msa0600180-mRNA-1:cds;Parent=Msa0600180-mRNA-1
chr4_2 CDS 41069290 41069985 41069290 ID=Msa0600180-mRNA-1:cds;Parent=Msa0600180-mRNA-1
chr4_2 CDS 41069076 41069189 41069076 ID=Msa0600180-mRNA-1:cds;Parent=Msa0600180-mRNA-1
chr4_2 CDS 41067677 41068417 41067677 ID=Msa0600180-mRNA-1:cds;Parent=Msa0600180-mRNA-1
Gene Sequence

>Msa0600180

ATGGCTTCAGTTCCGGTTAATAACAACTCTTTCCCTGATGATACTAACAACCTTGAGTTGAAACTTAGTCCCAACAAAAACATGGATGCTACACATAGTACTATCCCTTTCCTAGTCAATATAAGGTCCTCCAATGAAAATCAAACCTCTCCAATCTCATCTCATGAAACAAGAACAGCGTTGGATTTCTTTGATGACAACAACAATGACCATCATGAAGTTGTCTCTGCCTCTGTCCCTGACAATGATCATATTCATACTGATACACCATCTTTATTGGAACTAAAGCTAAGCCTTGGTCCCATTCCTATTACTATCAATACTAGTAGTGATCAATCTATGATGGATGATGGCATGTCACCTAATTCAGAAGAGAAGAGAACTAAGAGTATGGAGATGGCTATTCTTCAAGGAGAGCTTGAACAAACGAAAATGGAGAACTGTAGACTAAAATTGATGTATGATCTGCTTAGAATAGATTACAATTACATGCGGAAGCGTTTCGAGAGAGTGATGCAAGACCGTAAGGGTAAGAAAATTAAAGGAAAAAAAGTCTTTGATGGAAAATTTAAAGAGAAAAAGAGAACTGAGAATGGTGGAGTACTGGGGCCAAGGAAATTTATGGAACTTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGACAAAAAGTTTCTGATAAAAAATTTGTAGAGAAAATGAGAATTAAGAATGATGGAGAATTAATGAAAAGGAAATTTGATGATGCTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGAAAAGAAGTGTTCACTGGAAAATGTGAAAAGAAAAAGAGACTTCAGAATGGTGGAGAGTTGGTGTCAAGGCAATGTAAGGATCTCGTGTTGATTACAAATGCTGATATTGTGATGGATCACGAAGCTTCGTCCTCGTCAATGAGAAAACCTAGAAGTCAAGATCAGTTAGGATCGGCGATGAAAAACATCGATGTTGCTTCGAATGAGATAGTCCTAAGTAAGAATGAGGATGTTAATGTTGATAATGTTGAGGATTCATTGACAAAAGCAAGAGTTTGTATTAAAGCTAGATCAGAAGAAACCATGATCACTGATGGATGTCAATGGAGAAAGTATGGGCAAAAACTATCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACGACCCATGGTTGCCCAGTCCGAAAGCATGTACAAAGGTGTGCTTTTGACAAAACAGTTGTAATCACAACTTATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCAGCAGCTGCAAAGATACTTCTTTCAGCATCAACATCAAGCAATGATGGACAATTAAATGAAAATTTACTCACAAGAACCCTTCTTCCTTGTTCTTCAAGTATTGCAACTATATCAGCTTCTGCTCCATATCCAACTATTACAATTGATTACACTCAATCTCCAAATACTCCACAAAGGAACGCAAATCAGTTTCAAACTCCTTTAATCCCTCAGAGTTTTGGAAATTCCTCAGCTTCTCTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACATATGTCGAAAGATGCAGCTGACCCTTCACAAATATTGGCAATACCAAATATTACTGCTCAAACAGTTAATGCTGCCATTGCTGCTAGTCCAAATATTCCTGCAGCTTTGTCAGCTGCTATTACATCCATTATTGGTGATACTCAGCCAAACAACAACACTGCTGCTGCTGATAATAATAATAATGGCAACAATGTCACCGAAGGAAATAGCAATGGTAGCAACAATAATGTCACAGAAGCCAATAGCAACAAAATTATCACATGTGGCATCAACAATGATGGGAAGGAACAAAATCAGGAATACCAATTACAAGACCTAAACTTTAATTTTTTTTGCTCAAAGATTTAG
Protein sequence

>Msa0600180

MASVPVNNNSFPDDTNNLELKLSPNKNMDATHSTIPFLVNIRSSNENQTSPISSHETRTALDFFDDNNNDHHEVVSASVPDNDHIHTDTPSLLELKLSLGPIPITINTSSDQSMMDDGMSPNSEEKRTKSMEMAILQGELEQTKMENCRLKLMYDLLRIDYNYMRKRFERVMQDRKGKKIKGKKVFDGKFKEKKRTENGGVLGPRKFMELGLATDKVKEVKGQKVSDKKFVEKMRIKNDGELMKRKFDDAGLATDKVKEVKGKEVFTGKCEKKKRLQNGGELVSRQCKDLVLITNADIVMDHEASSSSMRKPRSQDQLGSAMKNIDVASNEIVLSKNEDVNVDNVEDSLTKARVCIKARSEETMITDGCQWRKYGQKLSKGNPCPKAYYRCSTTHGCPVRKHVQRCAFDKTVVITTYEGNHNHPLPAAAKEMAKTTSAAAKILLSASTSSNDGQLNENLLTRTLLPCSSSIATISASAPYPTITIDYTQSPNTPQRNANQFQTPLIPQSFGNSSASLIPQIPNQNQSKFSGLHMSKDAADPSQILAIPNITAQTVNAAIAASPNIPAALSAAITSIIGDTQPNNNTAAADNNNNGNNVTEGNSNGSNNNVTEANSNKIITCGINNDGKEQNQEYQLQDLNFNFFCSKI*