Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0604870 | XP_013456334.1 | 98.841 | 1122 | 13 | 0 | 1 | 1122 | 1 | 1122 | 0.0 | 2284 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0604870 | sp|Q9ZQ31|STY13_ARATH | 26.453 | 344 | 169 | 12 | 780 | 1107 | 109 | 384 | 4.59e-22 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0604870 | A0A072UX07 | 98.841 | 1122 | 13 | 0 | 1 | 1122 | 1 | 1122 | 0.0 | 2284 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0604870 | MtrunA17_Chr4g0034221 | 98.841 | 1122 | 13 | 0 | 1 | 1122 | 1 | 1122 | 0.0 | 2284 |
| Msa0604870 | MtrunA17_Chr4g0051191 | 27.469 | 324 | 166 | 10 | 782 | 1105 | 159 | 413 | 1.10e-21 | 99.4 |
| Msa0604870 | MtrunA17_Chr3g0090261 | 28.076 | 317 | 143 | 12 | 800 | 1107 | 96 | 336 | 2.28e-21 | 97.4 |
| Msa0604870 | MtrunA17_Chr2g0282321 | 31.034 | 232 | 121 | 7 | 796 | 1027 | 171 | 363 | 3.50e-21 | 98.2 |
| Msa0604870 | MtrunA17_Chr3g0115171 | 29.530 | 298 | 138 | 11 | 806 | 1103 | 305 | 530 | 4.06e-21 | 99.0 |
| Msa0604870 | MtrunA17_Chr5g0424501 | 30.483 | 269 | 115 | 12 | 837 | 1103 | 241 | 439 | 3.25e-20 | 95.5 |
| Msa0604870 | MtrunA17_Chr3g0133541 | 27.844 | 334 | 152 | 13 | 802 | 1121 | 315 | 573 | 9.74e-20 | 95.1 |
| Msa0604870 | MtrunA17_Chr3g0117731 | 28.990 | 307 | 149 | 10 | 812 | 1116 | 81 | 320 | 1.20e-19 | 92.0 |
| Msa0604870 | MtrunA17_Chr5g0424581 | 30.483 | 269 | 115 | 12 | 837 | 1103 | 174 | 372 | 3.63e-19 | 91.3 |
| Msa0604870 | MtrunA17_Chr5g0424651 | 30.258 | 271 | 113 | 12 | 837 | 1103 | 370 | 568 | 5.73e-19 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0604870 | AT1G04210.1 | 55.160 | 1095 | 461 | 12 | 33 | 1113 | 33 | 1111 | 0.0 | 1140 |
| Msa0604870 | AT4G31170.7 | 26.899 | 316 | 164 | 10 | 792 | 1107 | 137 | 385 | 3.16e-23 | 104 |
| Msa0604870 | AT4G31170.6 | 26.899 | 316 | 164 | 10 | 792 | 1107 | 137 | 385 | 3.16e-23 | 104 |
| Msa0604870 | AT4G31170.5 | 26.899 | 316 | 164 | 10 | 792 | 1107 | 137 | 385 | 3.16e-23 | 104 |
| Msa0604870 | AT4G31170.1 | 26.899 | 316 | 164 | 10 | 792 | 1107 | 137 | 385 | 3.16e-23 | 104 |
| Msa0604870 | AT4G31170.2 | 26.899 | 316 | 164 | 10 | 792 | 1107 | 137 | 385 | 3.16e-23 | 104 |
| Msa0604870 | AT4G31170.3 | 26.899 | 316 | 164 | 10 | 792 | 1107 | 137 | 385 | 3.16e-23 | 104 |
| Msa0604870 | AT4G31170.8 | 26.899 | 316 | 164 | 10 | 792 | 1107 | 137 | 385 | 3.16e-23 | 104 |
| Msa0604870 | AT2G24360.1 | 26.453 | 344 | 169 | 12 | 780 | 1107 | 109 | 384 | 4.67e-23 | 103 |
| Msa0604870 | AT1G63700.1 | 28.090 | 267 | 126 | 9 | 837 | 1103 | 450 | 650 | 3.88e-20 | 97.4 |
Find 274 sgRNAs with CRISPR-Local
Find 652 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATCTACTGTTAGATG+AGG | 0.669477 | 4_2:-50636662 | None:intergenic |
| AAAATCACAGAGCTTCACAG+TGG | 0.725075 | 4_2:-50640224 | None:intergenic |
| AAAATGATATTCCAGGCATG+TGG | 0.666273 | 4_2:-50638143 | None:intergenic |
| AAACAAAGAGGCATTCAGAT+CGG | 0.509562 | 4_2:+50637136 | Msa0604870:CDS |
| AAACCCTTCAATTTATGCAA+CGG | 0.543522 | 4_2:-50633244 | None:intergenic |
| AAACTTTGAATATAATTCTT+TGG | 0.223578 | 4_2:+50639777 | Msa0604870:CDS |
| AAAGAGCTTGAGCTTTCTAA+GGG | 0.551657 | 4_2:+50633268 | Msa0604870:CDS |
| AAATACAACCAACAAACCCA+TGG | 0.590879 | 4_2:-50642049 | None:intergenic |
| AAATCACAGAGCTTCACAGT+GGG | 0.643973 | 4_2:-50640223 | None:intergenic |
| AAATCTGGAACTTCCGGTAC+CGG | 0.492836 | 4_2:+50633015 | Msa0604870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TACTTCAACCTCTAAATTCT+AGG | + | chr4_2:50632779-50632798 | Msa0604870:five_prime_UTR | 30.0% | |
| ACTTCAACCTCTAAATTCTA+GGG | + | chr4_2:50632780-50632799 | Msa0604870:five_prime_UTR | 30.0% | |
| TTGAAAACGCTGAAATTCTT+TGG | + | chr4_2:50633127-50633146 | Msa0604870:CDS | 30.0% | |
| TGAGATTAATTTATTTGCGC+CGG | + | chr4_2:50633153-50633172 | Msa0604870:CDS | 30.0% | |
| AAACCCTTCAATTTATGCAA+CGG | - | chr4_2:50633247-50633266 | None:intergenic | 30.0% | |
| GCAAAAAAATGTGTCTTCTA+TGG | + | chr4_2:50633696-50633715 | Msa0604870:intron | 30.0% | |
| CATCAATAACAAACACTAAC+TGG | - | chr4_2:50634099-50634118 | None:intergenic | 30.0% | |
| TTCAAAGAACTAATCTCTGA+CGG | - | chr4_2:50634290-50634309 | None:intergenic | 30.0% | |
| GAAAGTTGCGAATAATAAGT+TGG | + | chr4_2:50634325-50634344 | Msa0604870:CDS | 30.0% | |
| AAGTCAACCTATTATTTGAC+AGG | - | chr4_2:50634400-50634419 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 50632754 | 50642119 | 50632754 | ID=Msa0604870;Name=Msa0604870 |
| chr4_2 | mRNA | 50632754 | 50642119 | 50632754 | ID=Msa0604870-mRNA-1;Parent=Msa0604870;Name=Msa0604870-mRNA-1;_AED=0.06;_eAED=0.06;_QI=130|1|1|1|1|1|11|484|1122 |
| chr4_2 | exon | 50632754 | 50633380 | 50632754 | ID=Msa0604870-mRNA-1:exon:11926;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50634211 | 50634475 | 50634211 | ID=Msa0604870-mRNA-1:exon:11927;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50636343 | 50636502 | 50636343 | ID=Msa0604870-mRNA-1:exon:11928;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50636626 | 50637370 | 50636626 | ID=Msa0604870-mRNA-1:exon:11929;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50637533 | 50637962 | 50637533 | ID=Msa0604870-mRNA-1:exon:11930;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50638077 | 50638258 | 50638077 | ID=Msa0604870-mRNA-1:exon:11931;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50639443 | 50639602 | 50639443 | ID=Msa0604870-mRNA-1:exon:11932;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50639733 | 50639960 | 50639733 | ID=Msa0604870-mRNA-1:exon:11933;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50640043 | 50640393 | 50640043 | ID=Msa0604870-mRNA-1:exon:11934;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50641175 | 50641276 | 50641175 | ID=Msa0604870-mRNA-1:exon:11935;Parent=Msa0604870-mRNA-1 |
| chr4_2 | exon | 50641387 | 50642119 | 50641387 | ID=Msa0604870-mRNA-1:exon:11936;Parent=Msa0604870-mRNA-1 |
| chr4_2 | five_prime_UTR | 50632754 | 50632883 | 50632754 | ID=Msa0604870-mRNA-1:five_prime_utr;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50632884 | 50633380 | 50632884 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50634211 | 50634475 | 50634211 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50636343 | 50636502 | 50636343 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50636626 | 50637370 | 50636626 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50637533 | 50637962 | 50637533 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50638077 | 50638258 | 50638077 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50639443 | 50639602 | 50639443 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50639733 | 50639960 | 50639733 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50640043 | 50640393 | 50640043 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50641175 | 50641276 | 50641175 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | CDS | 50641387 | 50641635 | 50641387 | ID=Msa0604870-mRNA-1:cds;Parent=Msa0604870-mRNA-1 |
| chr4_2 | three_prime_UTR | 50641636 | 50642119 | 50641636 | ID=Msa0604870-mRNA-1:three_prime_utr;Parent=Msa0604870-mRNA-1 |
| Gene Sequence |
>Msa0604870 ATGGAAATTCTCCCCTCCGACGAAACCCCACCGGAAACCTCCGCTATTCCCGAAAACCCCACCACTCCGATCGATGATTCCCCAAACCCTACCGGAATCGAAGAAGGTGTAACCCTCGACGTATCGGGAAAAAATCTGGAACTTCCGGTACCGGAGAATTCTTCAGACGATGCAGTGGAGAGTTTGTACATGTACAAGAACGTTTACAGTTTGATTCCGAAGTCGGTGGGTGGTTTAATGCGTTTGAAAACGCTGAAATTCTTTGGAAATGAGATTAATTTATTTGCGCCGGAGTTTGGGAACATGACGAGGTTAGAGAGGTTGCAGATGAAGGTATCTTCGCCGGGAATTGGTGGGTTGCCGTTGCATAAATTGAAGGGTTTGAAAGAGCTTGAGCTTTCTAAGGGTCCTTCTAGACCTTCTGCTTTTCCTATTTTGACTGAGATTGCTGCTCTCACGTGCTTGACCAAGCTTTGTATTTGTCACTTCAATATAAGATATCTTCCTCCGGAAATTGGGTGCTTAACGAATCTGGAATATCTTGATCTTTCATTCAATAAAATGAAGACATTGCCGTCAGAGATTAGTTCTTTGAAAGCATTAATATCGATGAAAGTTGCGAATAATAAGTTGGTGGAGTTACCGCCGGCTTTGACCTCACTATCAAGGTTGGAGAGTTTGGACCTGTCAAATAATAGGTTGACTTCGTTAGGATCACTTGAACTTAGCTCAATGCATAGACTTCAGAACTTAAGTCTTCAGCACAACAAGCTGCCCAGCATTTTTCAAATTCCTTCATGGATATGCTGTAATATGGAAGGAAATGATGGTGATGGATGCAAAGACAGTTATAGCAGTTCTTCTGTTGAAATGGATGTCTATGAAAGCAATTTGCCGGAAAGTGAGGAAACCTTTTCTCATGGACCTGATACCATCTCTTCAAGCGTATTAACAAACTCCTCATCTAACAGTAGATGTTTTGCAGCCTGGAAGTCATCAGGTAAACGGTGGAAGCGGCGACACAGTAAACAGCATAAAACTCGTCAAGAGCAGAAAGCTCGTCAAGAGCGGTTGAATAACAGCAGGAAGTGGAAAGGTGTAGATCATGACCAGTTGTTAAGCAAGAAGATCCACAGAATTTCTGAACCAGAAAATCTGGATACTCTTGTCTCTGAAAACTGCACAGAAACTGTCTCAGACAATGGAAGCCTGGATGACAATCATAAAAAAATATTCTCGGAAGAAGCAGCTCAAAACAATTTAATTGATAATATCGACAATGATGAGGTAATCATTGAAAAGCAATTTTCTCAAGAAGATTGCTGCACTGCAGAAAGTAAAGACGAACGAGATGCATCCTCATGCTCCTTAGACAATGGGCAAAGTGAACAGGATGGAGCATCTTGTTCAGATTTTTCGAAGTGTAGTTTTAAAACAAAGAGGCATTCAGATCGGGATCTTGATAATCCTAAACCCTGCAAGTCTAGAAAACCGATTGGTGATGGCTCATTATTGTCTTCCAAGTACAGTAAAATTTCATTTTGTGGCATTGAGGACCATCTATCAGATGGCTTTTATGATGCGGGGCGTGATCGTCCGTTTATGCCTCTTGAGAATTACGAGCAAAATCAGTGTCTTGCTTCTCGTGAAGTCATCCTGTTAGACAGACAAAGGGATGAAGAATTGGATGCCATTCTGCTATCTGCTCAGGGACTGGTCTCTAATTTGAAGCAGTTAAATGGTTTAAACAAACCTGGGAGCCAGGATGAAGTTGATAACTTGCAGACAGCATCACTGCTTGCACTCTTTGTATCTGATCATTTTGGCGGAAGTGATAGAGGTGCTATTATAGAAAGGGCACGCAAATCTGTTTCTGGCTCAAACTATAGTAAGCCTTTTGTTTGTACATGTTCGACCGGAAGCAGCACGAGTATAAATGCTGCAACTGAACCAGTTGTTAATGCCATAGAAGATATCAATCTTTCTATGATCTCTGAAAAATCTCTTGATTCTATTAAAAAAAGGCGAAATTCAATCATTGTTCCAATTGGCTCTGTTCAGTACGGGGTCTGTAGGCACAGAGCTCTTCTTTTTAAGTATTTATGTGATCACATGGAGCCACCTGTACCTTGCGAGCTAGTTAGGGGTTACCTGGATTTTTCACCACATGCCTGGAATATCATTTTAATAAAGAGGAGTGATACATGGGTTCGAATGCTTGTTGATGCTTGCCGACCTTATGATATTAGAGAAGAGAAAGACACCGAATATTTCTGCAGGTACATTCCTCTTCGTCGAACCCAAATTCCTCTTTCAACTAGCATTCTTCCCAGTCCTGATAATTCCTTTCCATCTCTCTCTAATTGTGATGAACTTGAGAAAAAAGCTTTAACTACATTAGTTCGGTGCAAATATGGATCAGTTGAGGCTGCTGTGAAGGTGCGTACTTTGGAAGTACAGGAAAATTCAGCAGACAAAATAAAAAACTTTGAATATAATTCTTTGGGAGAAATCAGAATTCTTGGTGCTTTTAAACACCCCTGTATAGTGGAAATATATGGACACCAAATATCATGCATATGGACTATCTCAGCTGATGGTAATCCGAAGCATCGAGTGTTGAGATCTGCAATTTTTATGGAATATGTTAAAGGGGGCTCCTTAAAAACCTATCTAGAGAAGATGTCAGAAGCTGGTGAAAAGCATGTTCCTGTGGAGTTAGCGTTGTATATTGCCAAAGATGTCTCATGCGCTTTGTTGGAGCTGCATTCAAAGCACATAATTCACCGTGACATAAAAAGTGAAAACATTCTGTTCGATTGTGATAGGAAGAGAGATGATGGAACTCCCACTGTGAAGCTCTGTGATTTTGATAGTGCAGTGCCACTAAGATCACCTCTACATGCATGCTGTATTGCTCATGTAGGAACACCTCCTCCTTCTTTGTGCGTGGGAACACCTCGGTGGATGGCTCCAGAGGTCATGCGAACTATGTACAAGAAAAACACTTATGGATTGGAAGCTGATATTTGGTCATTTGGATGTTTACTTCTGGAGATGCTAACTTTGCAAATTCCATATTTTGGAGTACCTGATTCACACGTCCATGATAGTCTGCAGATGGGTAAACGACCACAACTAACTGATGAGCTGGAGGCATTGAGCTCAATGAATGAACCCACAATGATTCAATCTGGTGAAGAGCTGGAAAAATCAGATGCTGAGACAGATACATTGAAGTTCCTTGTTGATTTGTTTCATAGGTGCGTGGAAGAAAATCCAAATGAACGACCTACAGCTGAAGAAATCCATGAAATGCTGCTTGGACACACAAGTCGCATACAAGTTCAAAATGTTAGTGAGATTTAG |
| Protein sequence |
>Msa0604870 MEILPSDETPPETSAIPENPTTPIDDSPNPTGIEEGVTLDVSGKNLELPVPENSSDDAVESLYMYKNVYSLIPKSVGGLMRLKTLKFFGNEINLFAPEFGNMTRLERLQMKVSSPGIGGLPLHKLKGLKELELSKGPSRPSAFPILTEIAALTCLTKLCICHFNIRYLPPEIGCLTNLEYLDLSFNKMKTLPSEISSLKALISMKVANNKLVELPPALTSLSRLESLDLSNNRLTSLGSLELSSMHRLQNLSLQHNKLPSIFQIPSWICCNMEGNDGDGCKDSYSSSSVEMDVYESNLPESEETFSHGPDTISSSVLTNSSSNSRCFAAWKSSGKRWKRRHSKQHKTRQEQKARQERLNNSRKWKGVDHDQLLSKKIHRISEPENLDTLVSENCTETVSDNGSLDDNHKKIFSEEAAQNNLIDNIDNDEVIIEKQFSQEDCCTAESKDERDASSCSLDNGQSEQDGASCSDFSKCSFKTKRHSDRDLDNPKPCKSRKPIGDGSLLSSKYSKISFCGIEDHLSDGFYDAGRDRPFMPLENYEQNQCLASREVILLDRQRDEELDAILLSAQGLVSNLKQLNGLNKPGSQDEVDNLQTASLLALFVSDHFGGSDRGAIIERARKSVSGSNYSKPFVCTCSTGSSTSINAATEPVVNAIEDINLSMISEKSLDSIKKRRNSIIVPIGSVQYGVCRHRALLFKYLCDHMEPPVPCELVRGYLDFSPHAWNIILIKRSDTWVRMLVDACRPYDIREEKDTEYFCRYIPLRRTQIPLSTSILPSPDNSFPSLSNCDELEKKALTTLVRCKYGSVEAAVKVRTLEVQENSADKIKNFEYNSLGEIRILGAFKHPCIVEIYGHQISCIWTISADGNPKHRVLRSAIFMEYVKGGSLKTYLEKMSEAGEKHVPVELALYIAKDVSCALLELHSKHIIHRDIKSENILFDCDRKRDDGTPTVKLCDFDSAVPLRSPLHACCIAHVGTPPPSLCVGTPRWMAPEVMRTMYKKNTYGLEADIWSFGCLLLEMLTLQIPYFGVPDSHVHDSLQMGKRPQLTDELEALSSMNEPTMIQSGEELEKSDAETDTLKFLVDLFHRCVEENPNERPTAEEIHEMLLGHTSRIQVQNVSEI* |
