AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0607880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0607880 MtrunA17_Chr4g0039661 97.302 556 14 1 16 571 1 555 0.0 1109
Msa0607880 MtrunA17_Chr2g0319091 38.177 351 192 12 235 570 238 578 2.22e-53 191
Msa0607880 MtrunA17_Chr3g0101141 35.876 354 210 10 229 571 195 542 5.03e-53 189
Msa0607880 MtrunA17_Chr5g0445331 34.286 385 232 9 202 571 154 532 9.24e-53 188
Msa0607880 MtrunA17_Chr7g0245601 34.332 367 219 10 212 571 441 792 2.79e-52 191
Msa0607880 MtrunA17_Chr3g0110971 34.884 387 216 12 206 571 216 587 3.67e-52 188
Msa0607880 MtrunA17_Chr7g0224271 31.294 425 236 11 172 571 280 673 2.48e-51 187
Msa0607880 MtrunA17_Chr4g0038761 34.167 360 208 11 223 571 101 442 3.82e-51 182
Msa0607880 MtrunA17_Chr3g0125811 31.383 376 235 8 210 571 204 570 5.51e-50 181
Msa0607880 MtrunA17_Chr2g0306641 32.120 467 263 19 122 571 58 487 1.02e-49 179
Msa0607880 MtrunA17_Chr1g0159461 31.452 372 240 7 210 571 226 592 1.23e-49 181
Msa0607880 MtrunA17_Chr1g0183641 33.871 372 224 10 206 570 120 476 1.51e-49 178
Msa0607880 MtrunA17_Chr5g0447691 33.684 380 237 9 202 571 170 544 2.65e-49 179
Msa0607880 MtrunA17_Chr3g0114841 31.536 371 232 7 219 570 132 499 2.16e-44 164
Msa0607880 MtrunA17_Chr4g0057291 30.287 383 239 12 206 570 289 661 3.20e-44 167
Msa0607880 MtrunA17_Chr4g0057891 27.792 385 249 7 206 571 146 520 3.09e-43 162
Msa0607880 MtrunA17_Chr4g0076091 32.286 350 220 9 232 570 199 542 4.39e-43 162
Msa0607880 MtrunA17_Chr8g0376381 30.952 378 228 14 206 571 166 522 1.59e-42 160
Msa0607880 MtrunA17_Chr1g0200401 31.899 395 230 12 205 571 76 459 2.82e-42 158
Msa0607880 MtrunA17_Chr2g0290241 30.857 350 227 8 232 571 81 425 1.02e-41 155
Msa0607880 MtrunA17_Chr5g0424131 31.232 349 223 6 237 570 152 498 1.27e-38 148
Msa0607880 MtrunA17_Chr3g0085681 28.009 432 239 13 200 571 17 436 3.13e-38 146
Msa0607880 MtrunA17_Chr2g0328341 28.684 380 244 10 207 570 269 637 2.14e-37 147
Msa0607880 MtrunA17_Chr2g0328391 26.772 381 254 10 207 570 296 668 1.01e-36 145
Msa0607880 MtrunA17_Chr4g0031721 29.024 379 247 9 206 570 313 683 1.19e-36 145
Msa0607880 MtrunA17_Chr4g0031741 27.676 383 255 9 201 570 276 649 1.23e-36 144
Msa0607880 MtrunA17_Chr2g0328381 28.684 380 244 10 207 570 272 640 6.05e-36 142
Msa0607880 MtrunA17_Chr8g0357601 28.571 385 245 10 206 571 179 552 9.49e-36 141
Msa0607880 MtrunA17_Chr5g0401131 29.771 393 240 13 202 571 111 490 1.02e-35 140
Msa0607880 MtrunA17_Chr5g0396491 25.688 436 244 9 206 571 53 478 1.20e-35 139
Msa0607880 MtrunA17_Chr4g0031711 27.198 364 239 9 225 570 377 732 1.25e-35 142
Msa0607880 MtrunA17_Chr2g0294461 27.062 388 252 11 206 571 207 585 4.74e-35 139
Msa0607880 MtrunA17_Chr4g0038851 26.545 437 238 12 206 571 45 469 1.31e-34 136
Msa0607880 MtrunA17_Chr2g0294471 26.579 380 261 8 206 571 201 576 2.74e-34 137
Msa0607880 MtrunA17_Chr8g0383981 30.479 397 246 15 186 570 117 495 3.98e-34 135
Msa0607880 MtrunA17_Chr2g0328411 25.464 377 260 7 206 570 286 653 6.44e-34 137
Msa0607880 MtrunA17_Chr4g0031701 25.441 397 278 8 178 560 223 615 6.55e-34 136
Msa0607880 MtrunA17_Chr4g0031731 27.429 350 236 8 225 560 270 615 2.26e-33 134
Msa0607880 MtrunA17_Chr4g0012071 29.639 415 240 16 179 571 167 551 2.49e-33 134
Msa0607880 MtrunA17_Chr7g0238891 26.836 354 241 8 232 571 391 740 8.35e-33 134
Msa0607880 MtrunA17_Chr2g0328431 25.464 377 260 7 206 570 286 653 1.68e-32 132
Msa0607880 MtrunA17_Chr7g0270031 29.076 368 244 10 214 571 194 554 2.06e-32 131
Msa0607880 MtrunA17_Chr2g0328401 26.111 360 245 7 190 536 271 622 4.14e-32 131
Msa0607880 MtrunA17_Chr2g0328361 28.342 374 228 11 207 570 271 614 5.76e-32 130
Msa0607880 MtrunA17_Chr7g0230771 30.933 375 235 12 206 571 372 731 4.46e-31 128
Msa0607880 MtrunA17_Chr7g0230731 30.133 375 238 11 206 571 369 728 2.37e-30 126
Msa0607880 MtrunA17_Chr3g0085361 27.714 350 184 10 279 571 13 350 3.78e-30 121
Msa0607880 MtrunA17_Chr2g0329591 27.735 393 241 11 208 571 73 451 7.24e-29 119
Msa0607880 MtrunA17_Chr2g0328371 29.845 258 158 7 277 521 1 248 2.25e-28 114
Msa0607880 MtrunA17_Chr7g0236851 29.496 417 254 16 174 570 260 656 5.67e-24 106
Msa0607880 MtrunA17_Chr5g0401421 24.429 438 263 13 196 570 13 445 3.02e-23 102
Msa0607880 MtrunA17_Chr4g0053901 23.673 452 276 14 173 570 31 467 6.11e-20 92.8
Msa0607880 MtrunA17_Chr1g0194621 21.271 362 251 10 231 571 170 518 3.35e-19 90.9
Msa0607880 MtrunA17_Chr8g0344101 25.521 384 257 10 210 571 224 600 5.97e-19 90.5
Msa0607880 MtrunA17_Chr8g0373561 25.770 357 217 13 237 571 211 541 7.16e-16 80.9
Msa0607880 MtrunA17_Chr7g0243151 23.823 361 235 13 234 571 241 584 8.41e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0607880 AT5G66770.1 54.770 608 199 15 16 571 1 584 0.0 537
Msa0607880 AT3G50650.1 50.171 584 221 14 16 571 1 542 4.05e-163 476
Msa0607880 AT5G41920.1 32.843 408 233 12 182 571 16 400 6.01e-55 191
Msa0607880 AT5G48150.1 33.858 381 236 7 202 571 115 490 1.99e-53 189
Msa0607880 AT5G48150.4 33.858 381 236 7 202 571 115 490 1.99e-53 189
Msa0607880 AT5G48150.2 33.858 381 236 7 202 571 115 490 1.99e-53 189
Msa0607880 AT5G48150.3 33.858 381 236 7 202 571 115 490 1.99e-53 189
Msa0607880 AT1G50600.3 34.474 380 234 8 202 571 152 526 6.79e-53 189
Msa0607880 AT1G50600.1 34.474 380 234 8 202 571 223 597 2.29e-52 189
Msa0607880 AT3G03450.1 34.127 378 225 13 206 571 180 545 2.84e-52 187
Msa0607880 AT3G54220.1 33.784 370 218 11 212 570 296 649 3.64e-52 189
Msa0607880 AT1G50600.2 34.474 380 234 8 202 571 227 601 3.75e-52 188
Msa0607880 AT2G04890.1 34.085 355 210 12 228 571 72 413 5.84e-49 175
Msa0607880 AT5G17490.1 32.061 393 244 12 189 571 137 516 8.11e-46 169
Msa0607880 AT1G66350.1 29.149 470 276 16 124 571 72 506 3.67e-45 167
Msa0607880 AT1G21450.1 28.000 400 273 8 182 571 199 593 5.56e-44 165
Msa0607880 AT1G63100.1 31.776 321 184 8 272 570 321 628 4.26e-43 163
Msa0607880 AT1G63100.2 31.949 313 178 8 280 570 354 653 6.85e-43 163
Msa0607880 AT4G17230.1 31.649 376 242 8 206 571 155 525 3.98e-42 159
Msa0607880 AT4G17230.2 31.649 376 242 8 206 571 155 525 3.98e-42 159
Msa0607880 AT1G55580.1 31.842 380 204 9 239 571 74 445 7.22e-42 157
Msa0607880 AT2G01570.1 33.141 347 206 14 235 571 251 581 9.47e-42 159
Msa0607880 AT1G14920.1 31.519 349 209 12 235 571 199 529 7.50e-41 155
Msa0607880 AT3G46600.2 27.763 371 255 7 210 571 5 371 1.17e-39 149
Msa0607880 AT4G08250.1 29.429 350 237 5 229 570 129 476 6.55e-39 149
Msa0607880 AT3G46600.1 27.763 371 255 7 210 571 213 579 7.76e-39 150
Msa0607880 AT3G46600.3 27.763 371 255 7 210 571 181 547 1.19e-38 149
Msa0607880 AT1G07520.2 28.382 377 251 7 210 570 155 528 8.56e-38 147
Msa0607880 AT1G07520.4 28.382 377 251 7 210 570 155 528 8.56e-38 147
Msa0607880 AT4G37650.1 29.101 378 238 11 218 571 158 529 9.25e-38 147
Msa0607880 AT1G07520.3 28.382 377 251 7 210 570 320 693 9.78e-38 149
Msa0607880 AT1G07520.1 28.382 377 251 7 210 570 319 692 9.81e-38 148
Msa0607880 AT5G59450.1 26.551 403 278 8 183 571 200 598 1.55e-37 147
Msa0607880 AT1G07530.1 26.633 398 270 9 187 570 375 764 1.98e-36 145
Msa0607880 AT2G29065.1 27.763 389 239 10 210 570 253 627 9.65e-36 142
Msa0607880 AT2G29060.2 25.416 421 295 10 164 570 239 654 2.29e-35 141
Msa0607880 AT2G29060.1 25.178 421 296 10 164 570 276 691 2.55e-35 141
Msa0607880 AT2G37650.1 25.217 345 236 7 240 571 378 713 4.64e-28 119
Msa0607880 AT2G37650.2 25.217 345 236 7 240 571 366 701 5.55e-28 119
Msa0607880 AT4G00150.1 28.338 367 224 13 221 571 211 554 2.02e-26 114
Msa0607880 AT5G52510.1 26.615 387 248 12 208 571 267 640 2.45e-26 114
Msa0607880 AT1G50420.1 31.278 227 139 5 220 440 71 286 4.42e-24 106
Msa0607880 AT1G50420.1 32.414 145 90 3 432 571 338 479 8.13e-15 77.4
Msa0607880 AT2G45160.1 28.070 399 227 16 206 571 268 639 5.02e-24 107
Msa0607880 AT3G60630.1 28.488 344 220 9 242 571 291 622 7.89e-24 106
Msa0607880 AT3G49950.1 27.451 357 225 12 239 570 60 407 4.02e-23 102
Msa0607880 AT3G13840.1 25.459 381 250 9 208 571 147 510 2.88e-20 94.7
Msa0607880 AT4G36710.1 23.947 380 259 12 206 571 122 485 4.64e-17 84.7

Find 157 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4_2:-54876647 Msa0607880:CDS
AATATTCATATAGTTGATTT+TGG 0.139541 4_2:-54876734 Msa0607880:CDS
CTCTATTGACTGTTTCTTCT+TGG 0.170351 4_2:-54875977 Msa0607880:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4_2:+54876647 None:intergenic
ATATGGGCATGCATCGTTTA+AGG 0.214693 4_2:+54876817 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4_2:-54876438 Msa0607880:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4_2:+54876941 None:intergenic
CTCTGTAAATTTGATTTGTA+TGG 0.250531 4_2:-54875516 Msa0607880:three_prime_UTR
GGTATACCTGCTATGGCTTT+AGG 0.252034 4_2:-54876626 Msa0607880:CDS
TCACCTAAAGTAACAATCTT+AGG 0.256338 4_2:+54876362 None:intergenic
AAATGTGCGAACTTTGAATA+TGG 0.257358 4_2:+54876800 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4_2:+54876942 None:intergenic
TTGATGATATTGGCTCAAAT+TGG 0.286520 4_2:-54875947 Msa0607880:three_prime_UTR
AATCCTAAGATTGTTACTTT+AGG 0.287104 4_2:-54876365 Msa0607880:CDS
GTTGATGTTGCACTGGTATT+AGG 0.301823 4_2:-54875624 Msa0607880:three_prime_UTR
AAGGTTATGGACTTGTAAAA+TGG 0.302281 4_2:-54875588 Msa0607880:three_prime_UTR
TGTGGAGTGGGTGGTTGGAT+TGG 0.309124 4_2:+54877133 None:intergenic
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4_2:+54877001 None:intergenic
GTGGAGTGGGTGGTTGGATT+GGG 0.321937 4_2:+54877134 None:intergenic
TTCGATGAGTGAAGCAATTT+CGG 0.323142 4_2:+54877033 None:intergenic
CAAATTCTTCTTCAACTACT+TGG 0.323189 4_2:-54876865 Msa0607880:CDS
TATTTGTCTAAATATCAAAA+AGG 0.329898 4_2:-54875486 Msa0607880:three_prime_UTR
TAGGGCTTGTGAAAAGTAAA+AGG 0.330479 4_2:+54876925 None:intergenic
TAATTAAATTAATTATCGTT+TGG 0.333699 4_2:-54875738 Msa0607880:three_prime_UTR
TGGCGTTGATTTGATGATAT+TGG 0.335085 4_2:-54875957 Msa0607880:exon
AATTAAATTAATTATCGTTT+GGG 0.335442 4_2:-54875737 Msa0607880:three_prime_UTR
GGATTCGCCGGAGAGGTTTC+AGG 0.345000 4_2:-54876234 Msa0607880:CDS
CTTGTTGAGCTATTGCCATT+AGG 0.345192 4_2:+54877590 None:intergenic
GCTATCGGCGGCGGACATTA+AGG 0.345403 4_2:+54877252 None:intergenic
TCGATTTACTGAGATGGGTT+AGG 0.351223 4_2:+54876981 None:intergenic
TTGTGATACCTAGGATGTTT+TGG 0.353661 4_2:+54877503 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4_2:-54876674 Msa0607880:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.358736 4_2:+54876467 None:intergenic
ATTACATTTGTACCTGCCTT+GGG 0.362140 4_2:+54878541 None:intergenic
TTGAAATGGGTCGGTGAAAT+CGG 0.367983 4_2:+54877387 None:intergenic
GGACCCGACCCGACTTGAAA+TGG 0.372343 4_2:+54877373 None:intergenic
ACCCTCACTCAACCACCTTC+CGG 0.372404 4_2:-54877309 Msa0607880:CDS
AGAGAGATGTTGTTTATAGA+TGG 0.381205 4_2:-54878573 None:intergenic
ATCAACTATATGAATATTGT+TGG 0.382716 4_2:+54876739 None:intergenic
TATTACATTTGTACCTGCCT+TGG 0.386304 4_2:+54878540 None:intergenic
TAGTTCTTTGTATTCTCTTG+TGG 0.398410 4_2:-54876042 Msa0607880:CDS
TGAAAACAAAAGAAAGGAAA+AGG 0.401725 4_2:+54875678 None:intergenic
CGATTTACTGAGATGGGTTA+GGG 0.403048 4_2:+54876982 None:intergenic
CAAAGCAGTGATCAAGGTTA+TGG 0.404632 4_2:-54875601 Msa0607880:three_prime_UTR
TGAAAACGACGATGTCGTTT+CGG 0.406416 4_2:+54877087 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4_2:-54876200 Msa0607880:CDS
TGAGTGAAGCAATTTCGGTT+AGG 0.410733 4_2:+54877038 None:intergenic
GAGTGAAGCAATTTCGGTTA+GGG 0.413751 4_2:+54877039 None:intergenic
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4_2:-54876712 Msa0607880:CDS
CTATGCAGATTGGATATGTT+TGG 0.419938 4_2:-54875802 Msa0607880:three_prime_UTR
TATTGGCTCAAATTGGGAAA+AGG 0.422482 4_2:-54875940 Msa0607880:three_prime_UTR
CCGATTTCGACTCCGACGAC+TGG 0.423487 4_2:-54877282 Msa0607880:CDS
GACCCGACTCTCACCACAAC+CGG 0.424081 4_2:-54877191 Msa0607880:CDS
ATTGGCTCAAATTGGGAAAA+GGG 0.425217 4_2:-54875939 Msa0607880:three_prime_UTR
GGCGAATCCAACGCCATGTT+CGG 0.426377 4_2:+54876248 None:intergenic
TGATGATATTGGCTCAAATT+GGG 0.430939 4_2:-54875946 Msa0607880:three_prime_UTR
ATTTGAAGCGGTACGGTGGC+GGG 0.431027 4_2:+54877157 None:intergenic
GATTCATCAAGAAGCTCAAT+AGG 0.436690 4_2:+54876506 None:intergenic
GATTTGAAGCGGTACGGTGG+CGG 0.443082 4_2:+54877156 None:intergenic
TTTGGTGATGATGATGTTGT+TGG 0.448206 4_2:+54877521 None:intergenic
TGAAATCGATTTACTGAGAT+GGG 0.451988 4_2:+54876976 None:intergenic
CCAGTCGTCGGAGTCGAAAT+CGG 0.452148 4_2:+54877282 None:intergenic
ATCCGGTTGTGGTGAGAGTC+GGG 0.456439 4_2:+54877189 None:intergenic
TCACCGACCCATTTCAAGTC+GGG 0.456993 4_2:-54877381 Msa0607880:CDS
GTAGCTGAAATCGATGAAAC+TGG 0.464413 4_2:+54876596 None:intergenic
TGCGTTGTGGAGTGGGTGGT+TGG 0.467268 4_2:+54877128 None:intergenic
GAGTGAGGGTCGAGTGGTGA+AGG 0.470291 4_2:+54877322 None:intergenic
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4_2:-54876178 Msa0607880:CDS
AATTTCTGGTATACCTGCTA+TGG 0.471549 4_2:-54876633 Msa0607880:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4_2:-54876174 Msa0607880:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4_2:+54876801 None:intergenic
GAATTTGCAGGTGGTGGGAA+TGG 0.473934 4_2:-54877638 Msa0607880:intron
GACCCATTTCAAGTCGGGTC+GGG 0.475045 4_2:-54877376 Msa0607880:CDS
TCTGTAAATTTGATTTGTAT+GGG 0.480060 4_2:-54875515 Msa0607880:three_prime_UTR
GTTTCATCGATTTCAGCTAC+TGG 0.480562 4_2:-54876593 Msa0607880:CDS
AGTGATGTAGAAGAAGATAC+TGG 0.481681 4_2:+54877433 None:intergenic
GTGAAATCGATTTACTGAGA+TGG 0.489246 4_2:+54876975 None:intergenic
AGGTTATGGACTTGTAAAAT+GGG 0.491169 4_2:-54875587 Msa0607880:three_prime_UTR
CGGAGTCGAAATCGGAGAAC+CGG 0.495727 4_2:+54877290 None:intergenic
ATCAATTGAAAACAAAAGAA+AGG 0.496213 4_2:+54875672 None:intergenic
CGGGTCGGGCATGATGAAAA+CGG 0.497777 4_2:+54877208 None:intergenic
GAAAACAAAAGAAAGGAAAA+GGG 0.498963 4_2:+54875679 None:intergenic
ATCGGAGTGGCTATCGGCGG+CGG 0.500643 4_2:+54877243 None:intergenic
TGGCGAAACGAGCCCAAGGC+AGG 0.504353 4_2:-54878553 Msa0607880:intron
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4_2:-54876191 Msa0607880:CDS
GACCCGACCCGACTTGAAAT+GGG 0.507947 4_2:+54877374 None:intergenic
AAACGGGTTGAGATGGAAAT+CGG 0.511523 4_2:+54877225 None:intergenic
CACTGACAGTGGAAACCTAA+TGG 0.513258 4_2:-54877605 Msa0607880:CDS
GGGTCGGGCATGATGAAAAC+GGG 0.514429 4_2:+54877209 None:intergenic
TGAGAGTTTGGAACCGAACA+TGG 0.520432 4_2:-54876261 Msa0607880:CDS
AAATTCTTCTTCAACTACTT+GGG 0.520685 4_2:-54876864 Msa0607880:CDS
ACGTTGTTGTTGTTGTGCCA+TGG 0.520915 4_2:+54877457 None:intergenic
TTTGGAACCGAACATGGCGT+TGG 0.523106 4_2:-54876255 Msa0607880:CDS
GGTGAAGAAGCTATGCAGAT+TGG 0.523544 4_2:-54875812 Msa0607880:three_prime_UTR
CATCATCACCAAAACATCCT+AGG 0.524123 4_2:-54877511 Msa0607880:CDS
GGAAAAGGGTTTCTTCCACC+TGG 0.528044 4_2:-54875925 Msa0607880:three_prime_UTR
GATTGGGATTTGAAGCGGTA+CGG 0.528703 4_2:+54877150 None:intergenic
ATTAGGCAAAGCAGTGATCA+AGG 0.531199 4_2:-54875607 Msa0607880:three_prime_UTR
ATGGAAATCGGAGTGGCTAT+CGG 0.534057 4_2:+54877237 None:intergenic
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4_2:-54876711 Msa0607880:CDS
GTTGTGGTGAGAGTCGGGTC+GGG 0.536815 4_2:+54877194 None:intergenic
GTGGCGGGTAAGAGAAGATC+CGG 0.537694 4_2:+54877172 None:intergenic
AGCTCAATAGGCGTAAGAAT+CGG 0.539095 4_2:+54876518 None:intergenic
TGAAGCTAGTTTGACGACTC+GGG 0.539102 4_2:-54876336 Msa0607880:CDS
ATGGACTTGTAAAATGGGAA+TGG 0.542364 4_2:-54875582 Msa0607880:three_prime_UTR
TTTCGACTCCGACGACTGGA+TGG 0.543044 4_2:-54877278 Msa0607880:CDS
ATGAAGCTAGTTTGACGACT+CGG 0.543619 4_2:-54876337 Msa0607880:CDS
GATCCGGTTGTGGTGAGAGT+CGG 0.547170 4_2:+54877188 None:intergenic
GGTGAAGGGAAGCTGAAAGC+GGG 0.548051 4_2:+54877337 None:intergenic
CAAGAAGAAACAGTCAATAG+AGG 0.549819 4_2:+54875978 None:intergenic
AGTAAATCGATTTCACAAAA+CGG 0.553570 4_2:-54876968 Msa0607880:CDS
GAACATGGCGTTGGATTCGC+CGG 0.557782 4_2:-54876246 Msa0607880:CDS
AGTGAGGGTCGAGTGGTGAA+GGG 0.560260 4_2:+54877323 None:intergenic
GCTAGTTTGACGACTCGGGT+TGG 0.562442 4_2:-54876332 Msa0607880:CDS
CGACCCATTTCAAGTCGGGT+CGG 0.565104 4_2:-54877377 Msa0607880:CDS
AACCGGAAGGTGGTTGAGTG+AGG 0.565488 4_2:+54877307 None:intergenic
TCTCTGTCTCACTACATCCA+TGG 0.569966 4_2:-54877474 Msa0607880:CDS
AGGGAAGCTGAAAGCGGGAT+CGG 0.570525 4_2:+54877342 None:intergenic
GTAGCATGTTGATGTTGCAC+TGG 0.570751 4_2:-54875631 Msa0607880:three_prime_UTR
GAAGTTCCTAAAGCCATAGC+AGG 0.575286 4_2:+54876620 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4_2:-54876157 Msa0607880:CDS
CTCTCCACCTGAAACCTCTC+CGG 0.581104 4_2:+54876227 None:intergenic
GGTTGTGGTGAGAGTCGGGT+CGG 0.584555 4_2:+54877193 None:intergenic
AGAGAGGGTTTGTGATACCT+AGG 0.592419 4_2:+54877494 None:intergenic
ATGATGAAAACGGGTTGAGA+TGG 0.594967 4_2:+54877218 None:intergenic
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4_2:-54876153 Msa0607880:CDS
GGTTGGATTGGGATTTGAAG+CGG 0.600133 4_2:+54877145 None:intergenic
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4_2:-54876171 Msa0607880:CDS
GGATGTAGTGAGACAGAGAG+AGG 0.601119 4_2:+54877478 None:intergenic
GCAATTCTTGAAGCAACAGA+AGG 0.601796 4_2:-54876764 Msa0607880:CDS
TTCACCGACCCATTTCAAGT+CGG 0.604924 4_2:-54877382 Msa0607880:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4_2:-54876183 Msa0607880:CDS
ACCGGAAGGTGGTTGAGTGA+GGG 0.606602 4_2:+54877308 None:intergenic
GTGGTTGAGTGAGGGTCGAG+TGG 0.609203 4_2:+54877316 None:intergenic
GTCAAGCTAAGATATTGTTG+TGG 0.613839 4_2:-54876076 Msa0607880:CDS
TGGGATTTGAAGCGGTACGG+TGG 0.615546 4_2:+54877153 None:intergenic
TAAGGTATCCATCCAGTCGT+CGG 0.619410 4_2:+54877270 None:intergenic
AAAGAAAGGAAAAGGGTGTG+TGG 0.623975 4_2:+54875686 None:intergenic
AATGGGTCGGTGAAATCGGT+AGG 0.625917 4_2:+54877391 None:intergenic
TTCGCCGGAGAGGTTTCAGG+TGG 0.626303 4_2:-54876231 Msa0607880:CDS
GGTAAGAGAAGATCCGGTTG+TGG 0.627421 4_2:+54877178 None:intergenic
GAAATCGGAGAACCGGAAGG+TGG 0.633501 4_2:+54877297 None:intergenic
GATGTAGTGAGACAGAGAGA+GGG 0.639502 4_2:+54877479 None:intergenic
TAGATGGCGAAACGAGCCCA+AGG 0.643090 4_2:-54878557 Msa0607880:CDS
GGGTCGGTGAAATCGGTAGG+TGG 0.649301 4_2:+54877394 None:intergenic
TGGCGTTGGATTCGCCGGAG+AGG 0.656610 4_2:-54876241 Msa0607880:CDS
GCTTACATGTGCACTGACAG+TGG 0.657212 4_2:-54877616 Msa0607880:CDS
GTCGAAATCGGAGAACCGGA+AGG 0.658754 4_2:+54877294 None:intergenic
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4_2:-54876184 Msa0607880:CDS
TGGTGAAGGGAAGCTGAAAG+CGG 0.662293 4_2:+54877336 None:intergenic
CGACCCGACTTGAAATGGGT+CGG 0.673110 4_2:+54877378 None:intergenic
GAAATCGGAGTGGCTATCGG+CGG 0.678873 4_2:+54877240 None:intergenic
TCGGTAGGTGGAAAAGACAA+AGG 0.681946 4_2:+54877406 None:intergenic
AGAAGCTATTGAAGATTGTG+AGG 0.683841 4_2:+54876889 None:intergenic
AAACGACGATGTCGTTTCGG+TGG 0.687537 4_2:+54877090 None:intergenic
GGTTGAGATGGAAATCGGAG+TGG 0.716922 4_2:+54877230 None:intergenic
GTAGGTGGAAAAGACAAAGG+TGG 0.720227 4_2:+54877409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAATTAATTATCGTT+TGG - chr4_2:54878297-54878316 Msa0607880:intron 10.0%
!! AATTAAATTAATTATCGTTT+GGG - chr4_2:54878298-54878317 Msa0607880:intron 10.0%
!!! AATATTCATATAGTTGATTT+TGG - chr4_2:54877301-54877320 Msa0607880:CDS 15.0%
!!! TTGATTTTCCTTTTTTTTTT+TGG - chr4_2:54878381-54878400 Msa0607880:intron 15.0%
!!! TGATTTTCCTTTTTTTTTTT+GGG - chr4_2:54878382-54878401 Msa0607880:intron 15.0%
!! TATTTGTCTAAATATCAAAA+AGG - chr4_2:54878549-54878568 Msa0607880:intron 15.0%
!! TTTATATGATGTTGTATTGT+GGG - chr4_2:54875654-54875673 Msa0607880:three_prime_UTR 20.0%
!! AAATAAAAAATGTAAGAGAG+TGG - chr4_2:54876036-54876055 Msa0607880:CDS 20.0%
!! AATAAAAAATGTAAGAGAGT+GGG - chr4_2:54876037-54876056 Msa0607880:CDS 20.0%
!!! ATGAAACTGTCTTTTAAAAT+GGG + chr4_2:54876089-54876108 None:intergenic 20.0%
!!! AATGAAACTGTCTTTTAAAA+TGG + chr4_2:54876090-54876109 None:intergenic 20.0%
!! ATCAACTATATGAATATTGT+TGG + chr4_2:54877299-54877318 None:intergenic 20.0%
!! ATCAATTGAAAACAAAAGAA+AGG + chr4_2:54878366-54878385 None:intergenic 20.0%
!!! TCTGTAAATTTGATTTGTAT+GGG - chr4_2:54878520-54878539 Msa0607880:intron 20.0%
! GTTTATATGATGTTGTATTG+TGG - chr4_2:54875653-54875672 Msa0607880:three_prime_UTR 25.0%
! CAAAATCCTTATTCTCAAAT+TGG + chr4_2:54875690-54875709 None:intergenic 25.0%
! AAATGTGAGTTAGTTAAAGA+TGG + chr4_2:54875874-54875893 None:intergenic 25.0%
! AACACCAAAGAAATATAAGA+CGG + chr4_2:54875910-54875929 None:intergenic 25.0%
!!! ACTGAATATTTTGAATTTGC+AGG - chr4_2:54876385-54876404 Msa0607880:CDS 25.0%
!!! AATTGCTTGATTTGGTTTTT+GGG + chr4_2:54877029-54877048 None:intergenic 25.0%
!! AGTAAATCGATTTCACAAAA+CGG - chr4_2:54877067-54877086 Msa0607880:CDS 25.0%
! AAAAGGAAACTCTTTGATTA+GGG + chr4_2:54877096-54877115 None:intergenic 25.0%
! TAAAAGGAAACTCTTTGATT+AGG + chr4_2:54877097-54877116 None:intergenic 25.0%
! AAATTCTTCTTCAACTACTT+GGG - chr4_2:54877171-54877190 Msa0607880:CDS 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG - chr4_2:54877314-54877333 Msa0607880:CDS 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG - chr4_2:54877670-54877689 Msa0607880:intron 25.0%
!! TCAATTCATAATGTTTTGCT+TGG - chr4_2:54878202-54878221 Msa0607880:intron 25.0%
! GAAAACAAAAGAAAGGAAAA+GGG + chr4_2:54878359-54878378 None:intergenic 25.0%
! TGAAAACAAAAGAAAGGAAA+AGG + chr4_2:54878360-54878379 None:intergenic 25.0%
!!! CTCTGTAAATTTGATTTGTA+TGG - chr4_2:54878519-54878538 Msa0607880:intron 25.0%
CAGGTACAAATGTAATATAG+AGG - chr4_2:54875501-54875520 Msa0607880:three_prime_UTR 30.0%
!! TCTCAATCCCAATTTTGTTT+CGG + chr4_2:54875543-54875562 None:intergenic 30.0%
ATATGTATTCGCAGTAACAA+TGG - chr4_2:54875562-54875581 Msa0607880:three_prime_UTR 30.0%
TGATCTCCAATTTGAGAATA+AGG - chr4_2:54875681-54875700 Msa0607880:three_prime_UTR 30.0%
TTGTCTATACTCAAAGAAAG+CGG - chr4_2:54875707-54875726 Msa0607880:three_prime_UTR 30.0%
! AAACAAAGTGCTTCCAAATT+CGG + chr4_2:54875765-54875784 None:intergenic 30.0%
TTATCATCGTTAAAGTGTGA+GGG + chr4_2:54875850-54875869 None:intergenic 30.0%
ATTATCATCGTTAAAGTGTG+AGG + chr4_2:54875851-54875870 None:intergenic 30.0%
TACACCGTCTTATATTTCTT+TGG - chr4_2:54875903-54875922 Msa0607880:three_prime_UTR 30.0%
TTATAGTCGTTCAATGTTCT+AGG - chr4_2:54876110-54876129 Msa0607880:CDS 30.0%
!! ATGATGATGGTGTAATAGAT+TGG + chr4_2:54876291-54876310 None:intergenic 30.0%
TAATTAGATGTATGAGAGAG+AGG + chr4_2:54876332-54876351 None:intergenic 30.0%
!! GAATATTTTGAATTTGCAGG+TGG - chr4_2:54876388-54876407 Msa0607880:CDS 30.0%
!!! GTTGTTCTTGTTGTTGTTTT+TGG + chr4_2:54876478-54876497 None:intergenic 30.0%
!!! CAATTGCTTGATTTGGTTTT+TGG + chr4_2:54877030-54877049 None:intergenic 30.0%
! TGAAATCGATTTACTGAGAT+GGG + chr4_2:54877062-54877081 None:intergenic 30.0%
CAAATTCTTCTTCAACTACT+TGG - chr4_2:54877170-54877189 Msa0607880:CDS 30.0%
AATGTGCGAACTTTGAATAT+GGG + chr4_2:54877237-54877256 None:intergenic 30.0%
AAATGTGCGAACTTTGAATA+TGG + chr4_2:54877238-54877257 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG - chr4_2:54877313-54877332 Msa0607880:CDS 30.0%
AATTGTTCAAGGGATTCAAT+GGG - chr4_2:54877324-54877343 Msa0607880:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG + chr4_2:54877391-54877410 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG - chr4_2:54877388-54877407 Msa0607880:CDS 30.0%
! GCTTCAGTTGTATAATCTTT+TGG - chr4_2:54877597-54877616 Msa0607880:CDS 30.0%
TCACCTAAAGTAACAATCTT+AGG + chr4_2:54877676-54877695 None:intergenic 30.0%
TAGTTCTTTGTATTCTCTTG+TGG - chr4_2:54877993-54878012 Msa0607880:intron 30.0%
!! TTGATGATATTGGCTCAAAT+TGG - chr4_2:54878088-54878107 Msa0607880:intron 30.0%
!! TGATGATATTGGCTCAAATT+GGG - chr4_2:54878089-54878108 Msa0607880:intron 30.0%
! ACTCTGCAATACAAGATTTT+TGG - chr4_2:54878268-54878287 Msa0607880:intron 30.0%
! CTCTGCAATACAAGATTTTT+GGG - chr4_2:54878269-54878288 Msa0607880:intron 30.0%
CATGCTACCCAAAAAAAAAA+AGG + chr4_2:54878392-54878411 None:intergenic 30.0%
AAGGTTATGGACTTGTAAAA+TGG - chr4_2:54878447-54878466 Msa0607880:intron 30.0%
AGGTTATGGACTTGTAAAAT+GGG - chr4_2:54878448-54878467 Msa0607880:intron 30.0%
ATTACATTTGTACCTGCCTT+GGG + chr4_2:54875497-54875516 None:intergenic 35.0%
TATTACATTTGTACCTGCCT+TGG + chr4_2:54875498-54875517 None:intergenic 35.0%
!! TCAATCCCAATTTTGTTTCG+GGG + chr4_2:54875541-54875560 None:intergenic 35.0%
!! CTCAATCCCAATTTTGTTTC+GGG + chr4_2:54875542-54875561 None:intergenic 35.0%
TCAACTTACATCGAACACAA+TGG + chr4_2:54875622-54875641 None:intergenic 35.0%
ATTGTGTTCGATGTAAGTTG+AGG - chr4_2:54875621-54875640 Msa0607880:three_prime_UTR 35.0%
CAACAATGACATACCGAATT+TGG - chr4_2:54875749-54875768 Msa0607880:three_prime_UTR 35.0%
CTAACTTGAGTTCGATTCTT+CGG + chr4_2:54875975-54875994 None:intergenic 35.0%
AATCGAACTCAAGTTAGTTG+CGG - chr4_2:54875978-54875997 Msa0607880:CDS 35.0%
AAATCACACACACACTTCAA+CGG - chr4_2:54876184-54876203 Msa0607880:CDS 35.0%
GAAGTAGAAAAGAGTGAGTA+AGG + chr4_2:54876237-54876256 None:intergenic 35.0%
! GATGGTGTAATAGATTGGTT+TGG + chr4_2:54876286-54876305 None:intergenic 35.0%
!! GTTATGGTGATTGATGATGA+TGG + chr4_2:54876304-54876323 None:intergenic 35.0%
TCATCATCAATCACCATAAC+AGG - chr4_2:54876304-54876323 Msa0607880:CDS 35.0%
!! TATTTTGAATTTGCAGGTGG+TGG - chr4_2:54876391-54876410 Msa0607880:CDS 35.0%
! ATTTTGAATTTGCAGGTGGT+GGG - chr4_2:54876392-54876411 Msa0607880:CDS 35.0%
!! TTTGGTGATGATGATGTTGT+TGG + chr4_2:54876517-54876536 None:intergenic 35.0%
! TTGTGATACCTAGGATGTTT+TGG + chr4_2:54876535-54876554 None:intergenic 35.0%
AGTGATGTAGAAGAAGATAC+TGG + chr4_2:54876605-54876624 None:intergenic 35.0%
TTCGATGAGTGAAGCAATTT+CGG + chr4_2:54877005-54877024 None:intergenic 35.0%
AGGGTTTCAATTGCTTGATT+TGG + chr4_2:54877037-54877056 None:intergenic 35.0%
! GTGAAATCGATTTACTGAGA+TGG + chr4_2:54877063-54877082 None:intergenic 35.0%
TAGGGCTTGTGAAAAGTAAA+AGG + chr4_2:54877113-54877132 None:intergenic 35.0%
!! TGAGGTGATTTTGTTGGTTA+GGG + chr4_2:54877131-54877150 None:intergenic 35.0%
! AGATTGTGAGGTGATTTTGT+TGG + chr4_2:54877137-54877156 None:intergenic 35.0%
AGAAGCTATTGAAGATTGTG+AGG + chr4_2:54877149-54877168 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG - chr4_2:54877323-54877342 Msa0607880:CDS 35.0%
!! AATTTCTGGTATACCTGCTA+TGG - chr4_2:54877402-54877421 Msa0607880:CDS 35.0%
GATTCATCAAGAAGCTCAAT+AGG + chr4_2:54877532-54877551 None:intergenic 35.0%
! CTTTTGTATTCAGCCAAATG+AGG - chr4_2:54877555-54877574 Msa0607880:CDS 35.0%
TTAACAGCTAAAGCCTCATT+TGG + chr4_2:54877571-54877590 None:intergenic 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG - chr4_2:54877762-54877781 Msa0607880:intron 35.0%
GTCAAGCTAAGATATTGTTG+TGG - chr4_2:54877959-54877978 Msa0607880:intron 35.0%
CAAGAAGAAACAGTCAATAG+AGG + chr4_2:54878060-54878079 None:intergenic 35.0%
! CTCTATTGACTGTTTCTTCT+TGG - chr4_2:54878058-54878077 Msa0607880:intron 35.0%
!! TGGCGTTGATTTGATGATAT+TGG - chr4_2:54878078-54878097 Msa0607880:intron 35.0%
!! TATTGGCTCAAATTGGGAAA+AGG - chr4_2:54878095-54878114 Msa0607880:intron 35.0%
! ATTGGCTCAAATTGGGAAAA+GGG - chr4_2:54878096-54878115 Msa0607880:intron 35.0%
ACACCAGAGAAAAAAAAACC+AGG + chr4_2:54878131-54878150 None:intergenic 35.0%
!!! TGGTTTTTTTTTCTCTGGTG+TGG - chr4_2:54878130-54878149 Msa0607880:intron 35.0%
CTATGCAGATTGGATATGTT+TGG - chr4_2:54878233-54878252 Msa0607880:intron 35.0%
!!! GGGTTGTATTTTTAGCAGTT+TGG - chr4_2:54878318-54878337 Msa0607880:intron 35.0%
ATGGACTTGTAAAATGGGAA+TGG - chr4_2:54878453-54878472 Msa0607880:intron 35.0%
TCTTTCCCCCGAAACAAAAT+TGG - chr4_2:54875532-54875551 Msa0607880:three_prime_UTR 40.0%
CTTTCCCCCGAAACAAAATT+GGG - chr4_2:54875533-54875552 Msa0607880:three_prime_UTR 40.0%
!! CAATCCCAATTTTGTTTCGG+GGG + chr4_2:54875540-54875559 None:intergenic 40.0%
TCTATACTCAAAGAAAGCGG+TGG - chr4_2:54875710-54875729 Msa0607880:three_prime_UTR 40.0%
AAAATGTAAGAGAGTGGGTG+TGG - chr4_2:54876042-54876061 Msa0607880:CDS 40.0%
AAATGTAAGAGAGTGGGTGT+GGG - chr4_2:54876043-54876062 Msa0607880:CDS 40.0%
GTTGAAGTGTGTGTGTGATT+TGG + chr4_2:54876185-54876204 None:intergenic 40.0%
CTTGTTGAGCTATTGCCATT+AGG + chr4_2:54876448-54876467 None:intergenic 40.0%
CATCATCACCAAAACATCCT+AGG - chr4_2:54876524-54876543 Msa0607880:CDS 40.0%
TTGAAATGGGTCGGTGAAAT+CGG + chr4_2:54876651-54876670 None:intergenic 40.0%
!! AAACGGGTTGAGATGGAAAT+CGG + chr4_2:54876813-54876832 None:intergenic 40.0%
!! ATGATGAAAACGGGTTGAGA+TGG + chr4_2:54876820-54876839 None:intergenic 40.0%
!!! TTAGTGTCGTTTTTGCGTTG+TGG + chr4_2:54876923-54876942 None:intergenic 40.0%
! TGAAAACGACGATGTCGTTT+CGG + chr4_2:54876951-54876970 None:intergenic 40.0%
!!! GGTTTTAAGAAGTGGGTTTG+TGG + chr4_2:54876978-54876997 None:intergenic 40.0%
!!! CGGTTAGGGTTTTAAGAAGT+GGG + chr4_2:54876985-54877004 None:intergenic 40.0%
!!! TCGGTTAGGGTTTTAAGAAG+TGG + chr4_2:54876986-54877005 None:intergenic 40.0%
GAGTGAAGCAATTTCGGTTA+GGG + chr4_2:54876999-54877018 None:intergenic 40.0%
TGAGTGAAGCAATTTCGGTT+AGG + chr4_2:54877000-54877019 None:intergenic 40.0%
!! CGATTTACTGAGATGGGTTA+GGG + chr4_2:54877056-54877075 None:intergenic 40.0%
! TCGATTTACTGAGATGGGTT+AGG + chr4_2:54877057-54877076 None:intergenic 40.0%
!! GTGAGGTGATTTTGTTGGTT+AGG + chr4_2:54877132-54877151 None:intergenic 40.0%
ATATGGGCATGCATCGTTTA+AGG + chr4_2:54877221-54877240 None:intergenic 40.0%
GCAATTCTTGAAGCAACAGA+AGG - chr4_2:54877271-54877290 Msa0607880:CDS 40.0%
GTAGCTGAAATCGATGAAAC+TGG + chr4_2:54877442-54877461 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+TGG - chr4_2:54877442-54877461 Msa0607880:CDS 40.0%
AGCTCAATAGGCGTAAGAAT+CGG + chr4_2:54877520-54877539 None:intergenic 40.0%
!!! CAAACGAAGCGCTTTTTCAA+CGG + chr4_2:54877638-54877657 None:intergenic 40.0%
! ATGAAGCTAGTTTGACGACT+CGG - chr4_2:54877698-54877717 Msa0607880:intron 40.0%
TTGCTTGGTAGGAGAATTGA+TGG - chr4_2:54877835-54877854 Msa0607880:intron 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG - chr4_2:54877844-54877863 Msa0607880:intron 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG - chr4_2:54877891-54877910 Msa0607880:intron 40.0%
!! AAGGTTTTGATGGAAAGCTG+TGG - chr4_2:54877901-54877920 Msa0607880:intron 40.0%
!!! AAGCTGTGGTTTTGAATCAG+TGG - chr4_2:54877915-54877934 Msa0607880:intron 40.0%
!!! AGCTGTGGTTTTGAATCAGT+GGG - chr4_2:54877916-54877935 Msa0607880:intron 40.0%
!!! TCAACCTGCTTTTTTGTCTC+TGG - chr4_2:54878020-54878039 Msa0607880:intron 40.0%
CCAGAGAAAAAAAAACCAGG+TGG + chr4_2:54878128-54878147 None:intergenic 40.0%
!!! CCACCTGGTTTTTTTTTCTC+TGG - chr4_2:54878125-54878144 Msa0607880:intron 40.0%
! AAAGAAAGGAAAAGGGTGTG+TGG + chr4_2:54878352-54878371 None:intergenic 40.0%
! GTTGATGTTGCACTGGTATT+AGG - chr4_2:54878411-54878430 Msa0607880:intron 40.0%
ATTAGGCAAAGCAGTGATCA+AGG - chr4_2:54878428-54878447 Msa0607880:intron 40.0%
CAAAGCAGTGATCAAGGTTA+TGG - chr4_2:54878434-54878453 Msa0607880:intron 40.0%
GTTTCGGGGGAAAGAAAAAG+AGG + chr4_2:54875527-54875546 None:intergenic 45.0%
TCAAGTTAGTTGCGGACATG+CGG - chr4_2:54875986-54876005 Msa0607880:CDS 45.0%
AAGGAAACACTCACTCACTC+GGG + chr4_2:54876144-54876163 None:intergenic 45.0%
CAAGGAAACACTCACTCACT+CGG + chr4_2:54876145-54876164 None:intergenic 45.0%
! ATTTGGAGTGGAGAGAAGAG+AGG + chr4_2:54876168-54876187 None:intergenic 45.0%
! AGTGTGTGTGTGATTTGGAG+TGG + chr4_2:54876180-54876199 None:intergenic 45.0%
AGTGAGTAAGGTGAGAGAGT+GGG + chr4_2:54876225-54876244 None:intergenic 45.0%
TGAGAGAGAGGAACCTGTTA+TGG + chr4_2:54876320-54876339 None:intergenic 45.0%
CACTGACAGTGGAAACCTAA+TGG - chr4_2:54876430-54876449 Msa0607880:CDS 45.0%
AGAGAGGGTTTGTGATACCT+AGG + chr4_2:54876544-54876563 None:intergenic 45.0%
GATGTAGTGAGACAGAGAGA+GGG + chr4_2:54876559-54876578 None:intergenic 45.0%
TCTCTGTCTCACTACATCCA+TGG - chr4_2:54876561-54876580 Msa0607880:CDS 45.0%
! ACGTTGTTGTTGTTGTGCCA+TGG + chr4_2:54876581-54876600 None:intergenic 45.0%
GTAGGTGGAAAAGACAAAGG+TGG + chr4_2:54876629-54876648 None:intergenic 45.0%
TCGGTAGGTGGAAAAGACAA+AGG + chr4_2:54876632-54876651 None:intergenic 45.0%
! TTCACCGACCCATTTCAAGT+CGG - chr4_2:54876653-54876672 Msa0607880:CDS 45.0%
! TAAGGTATCCATCCAGTCGT+CGG + chr4_2:54876768-54876787 None:intergenic 45.0%
ATGGAAATCGGAGTGGCTAT+CGG + chr4_2:54876801-54876820 None:intergenic 45.0%
! GATTGGGATTTGAAGCGGTA+CGG + chr4_2:54876888-54876907 None:intergenic 45.0%
! GGTTGGATTGGGATTTGAAG+CGG + chr4_2:54876893-54876912 None:intergenic 45.0%
!! TCGTTTTTGCGTTGTGGAGT+GGG + chr4_2:54876917-54876936 None:intergenic 45.0%
GCATTTGCAACTCGTTCTTC+TGG - chr4_2:54877361-54877380 Msa0607880:CDS 45.0%
GGTATACCTGCTATGGCTTT+AGG - chr4_2:54877409-54877428 Msa0607880:CDS 45.0%
GAAGTTCCTAAAGCCATAGC+AGG + chr4_2:54877418-54877437 None:intergenic 45.0%
!! CGTTGAAAAAGCGCTTCGTT+TGG - chr4_2:54877636-54877655 Msa0607880:intron 45.0%
! TGAAGCTAGTTTGACGACTC+GGG - chr4_2:54877699-54877718 Msa0607880:intron 45.0%
! TGAGAGTTTGGAACCGAACA+TGG - chr4_2:54877774-54877793 Msa0607880:intron 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG - chr4_2:54877824-54877843 Msa0607880:intron 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG - chr4_2:54877851-54877870 Msa0607880:intron 45.0%
! TGATGGTGTGATTGGTGTGA+GGG - chr4_2:54877852-54877871 Msa0607880:intron 45.0%
GGATGGAGGATAAAGAACAG+TGG - chr4_2:54877878-54877897 Msa0607880:intron 45.0%
GGAGGATAAAGAACAGTGGA+AGG - chr4_2:54877882-54877901 Msa0607880:intron 45.0%
CAAGCCAGAGACAAAAAAGC+AGG + chr4_2:54878027-54878046 None:intergenic 45.0%
!!! CTGCTTTTTTGTCTCTGGCT+TGG - chr4_2:54878025-54878044 Msa0607880:intron 45.0%
GGTGAAGAAGCTATGCAGAT+TGG - chr4_2:54878223-54878242 Msa0607880:intron 45.0%
GTAGCATGTTGATGTTGCAC+TGG - chr4_2:54878404-54878423 Msa0607880:intron 45.0%
AAACACTCACTCACTCGGGA+GGG + chr4_2:54876140-54876159 None:intergenic 50.0%
GAGTGAGTAAGGTGAGAGAG+TGG + chr4_2:54876226-54876245 None:intergenic 50.0%
!! GAATTTGCAGGTGGTGGGAA+TGG - chr4_2:54876397-54876416 Msa0607880:CDS 50.0%
! GCTTACATGTGCACTGACAG+TGG - chr4_2:54876419-54876438 Msa0607880:CDS 50.0%
GGATGTAGTGAGACAGAGAG+AGG + chr4_2:54876560-54876579 None:intergenic 50.0%
AATGGGTCGGTGAAATCGGT+AGG + chr4_2:54876647-54876666 None:intergenic 50.0%
! TCACCGACCCATTTCAAGTC+GGG - chr4_2:54876654-54876673 Msa0607880:CDS 50.0%
!! AAAGCGGGATCGGTGTTTTC+AGG + chr4_2:54876686-54876705 None:intergenic 50.0%
TGGTGAAGGGAAGCTGAAAG+CGG + chr4_2:54876702-54876721 None:intergenic 50.0%
GGTTGAGATGGAAATCGGAG+TGG + chr4_2:54876808-54876827 None:intergenic 50.0%
GGTAAGAGAAGATCCGGTTG+TGG + chr4_2:54876860-54876879 None:intergenic 50.0%
! TTTTTGCGTTGTGGAGTGGG+TGG + chr4_2:54876914-54876933 None:intergenic 50.0%
!!! GTCGTTTTTGCGTTGTGGAG+TGG + chr4_2:54876918-54876937 None:intergenic 50.0%
! AAACGACGATGTCGTTTCGG+TGG + chr4_2:54876948-54876967 None:intergenic 50.0%
! TTTGGAACCGAACATGGCGT+TGG - chr4_2:54877780-54877799 Msa0607880:intron 50.0%
! CAGGTGGAGAGTCTTTTGCT+TGG - chr4_2:54877820-54877839 Msa0607880:intron 50.0%
GGAAAAGGGTTTCTTCCACC+TGG - chr4_2:54878110-54878129 Msa0607880:intron 50.0%
TAGATGGCGAAACGAGCCCA+AGG - chr4_2:54875478-54875497 Msa0607880:three_prime_UTR 55.0%
GAAACACTCACTCACTCGGG+AGG + chr4_2:54876141-54876160 None:intergenic 55.0%
GAGTGGAGAGAAGAGAGGCA+AGG + chr4_2:54876163-54876182 None:intergenic 55.0%
! CGACCCGACTTGAAATGGGT+CGG + chr4_2:54876660-54876679 None:intergenic 55.0%
! CGACCCATTTCAAGTCGGGT+CGG - chr4_2:54876658-54876677 Msa0607880:CDS 55.0%
! GACCCATTTCAAGTCGGGTC+GGG - chr4_2:54876659-54876678 Msa0607880:CDS 55.0%
! GACCCGACCCGACTTGAAAT+GGG + chr4_2:54876664-54876683 None:intergenic 55.0%
AGGGAAGCTGAAAGCGGGAT+CGG + chr4_2:54876696-54876715 None:intergenic 55.0%
GGTGAAGGGAAGCTGAAAGC+GGG + chr4_2:54876701-54876720 None:intergenic 55.0%
AGTGAGGGTCGAGTGGTGAA+GGG + chr4_2:54876715-54876734 None:intergenic 55.0%
ACCCTCACTCAACCACCTTC+CGG - chr4_2:54876726-54876745 Msa0607880:CDS 55.0%
ACCGGAAGGTGGTTGAGTGA+GGG + chr4_2:54876730-54876749 None:intergenic 55.0%
AACCGGAAGGTGGTTGAGTG+AGG + chr4_2:54876731-54876750 None:intergenic 55.0%
GAAATCGGAGAACCGGAAGG+TGG + chr4_2:54876741-54876760 None:intergenic 55.0%
GTCGAAATCGGAGAACCGGA+AGG + chr4_2:54876744-54876763 None:intergenic 55.0%
CGGAGTCGAAATCGGAGAAC+CGG + chr4_2:54876748-54876767 None:intergenic 55.0%
CCAGTCGTCGGAGTCGAAAT+CGG + chr4_2:54876756-54876775 None:intergenic 55.0%
TTTCGACTCCGACGACTGGA+TGG - chr4_2:54876757-54876776 Msa0607880:CDS 55.0%
GAAATCGGAGTGGCTATCGG+CGG + chr4_2:54876798-54876817 None:intergenic 55.0%
GGGTCGGGCATGATGAAAAC+GGG + chr4_2:54876829-54876848 None:intergenic 55.0%
CGGGTCGGGCATGATGAAAA+CGG + chr4_2:54876830-54876849 None:intergenic 55.0%
ATCCGGTTGTGGTGAGAGTC+GGG + chr4_2:54876849-54876868 None:intergenic 55.0%
GATCCGGTTGTGGTGAGAGT+CGG + chr4_2:54876850-54876869 None:intergenic 55.0%
!! GTGGCGGGTAAGAGAAGATC+CGG + chr4_2:54876866-54876885 None:intergenic 55.0%
! ATTTGAAGCGGTACGGTGGC+GGG + chr4_2:54876881-54876900 None:intergenic 55.0%
! GATTTGAAGCGGTACGGTGG+CGG + chr4_2:54876882-54876901 None:intergenic 55.0%
! TGGGATTTGAAGCGGTACGG+TGG + chr4_2:54876885-54876904 None:intergenic 55.0%
GTGGAGTGGGTGGTTGGATT+GGG + chr4_2:54876904-54876923 None:intergenic 55.0%
TGTGGAGTGGGTGGTTGGAT+TGG + chr4_2:54876905-54876924 None:intergenic 55.0%
! GCTAGTTTGACGACTCGGGT+TGG - chr4_2:54877703-54877722 Msa0607880:intron 55.0%
GGCGAATCCAACGCCATGTT+CGG + chr4_2:54877790-54877809 None:intergenic 55.0%
! GAACATGGCGTTGGATTCGC+CGG - chr4_2:54877789-54877808 Msa0607880:intron 55.0%
CTCTCCACCTGAAACCTCTC+CGG + chr4_2:54877811-54877830 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG - chr4_2:54877857-54877876 Msa0607880:intron 55.0%
GATTGGTGTGAGGGAGAGGA+TGG - chr4_2:54877861-54877880 Msa0607880:intron 55.0%
! TGGGACCAAGAGCACGAGGA+TGG - chr4_2:54876062-54876081 Msa0607880:CDS 60.0%
TGGGACCATCCTCGTGCTCT+TGG + chr4_2:54876070-54876089 None:intergenic 60.0%
GGGTCGGTGAAATCGGTAGG+TGG + chr4_2:54876644-54876663 None:intergenic 60.0%
! GGACCCGACCCGACTTGAAA+TGG + chr4_2:54876665-54876684 None:intergenic 60.0%
GAGTGAGGGTCGAGTGGTGA+AGG + chr4_2:54876716-54876735 None:intergenic 60.0%
GTGGTTGAGTGAGGGTCGAG+TGG + chr4_2:54876722-54876741 None:intergenic 60.0%
CCGATTTCGACTCCGACGAC+TGG - chr4_2:54876753-54876772 Msa0607880:CDS 60.0%
GCTATCGGCGGCGGACATTA+AGG + chr4_2:54876786-54876805 None:intergenic 60.0%
GTTGTGGTGAGAGTCGGGTC+GGG + chr4_2:54876844-54876863 None:intergenic 60.0%
GGTTGTGGTGAGAGTCGGGT+CGG + chr4_2:54876845-54876864 None:intergenic 60.0%
GACCCGACTCTCACCACAAC+CGG - chr4_2:54876844-54876863 Msa0607880:CDS 60.0%
TGCGTTGTGGAGTGGGTGGT+TGG + chr4_2:54876910-54876929 None:intergenic 60.0%
GGATTCGCCGGAGAGGTTTC+AGG - chr4_2:54877801-54877820 Msa0607880:intron 60.0%
TTCGCCGGAGAGGTTTCAGG+TGG - chr4_2:54877804-54877823 Msa0607880:intron 60.0%
TGGTGTGAGGGAGAGGATGG+AGG - chr4_2:54877864-54877883 Msa0607880:intron 60.0%
TGGCGAAACGAGCCCAAGGC+AGG - chr4_2:54875482-54875501 Msa0607880:three_prime_UTR 65.0%
! GGTGTGGGACCAAGAGCACG+AGG - chr4_2:54876058-54876077 Msa0607880:CDS 65.0%
ATCGGAGTGGCTATCGGCGG+CGG + chr4_2:54876795-54876814 None:intergenic 65.0%
! TGGCGTTGGATTCGCCGGAG+AGG - chr4_2:54877794-54877813 Msa0607880:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 54875468 54878589 54875468 ID=Msa0607880;Name=Msa0607880
chr4_2 mRNA 54875468 54878589 54875468 ID=Msa0607880-mRNA-1;Parent=Msa0607880;Name=Msa0607880-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0|0|0.5|1|1|1|2|503|571
chr4_2 exon 54875468 54877650 54875468 ID=Msa0607880-mRNA-1:exon:13841;Parent=Msa0607880-mRNA-1
chr4_2 exon 54878554 54878589 54878554 ID=Msa0607880-mRNA-1:exon:13840;Parent=Msa0607880-mRNA-1
chr4_2 CDS 54878554 54878589 54878554 ID=Msa0607880-mRNA-1:cds;Parent=Msa0607880-mRNA-1
chr4_2 CDS 54875971 54877650 54875971 ID=Msa0607880-mRNA-1:cds;Parent=Msa0607880-mRNA-1
chr4_2 three_prime_UTR 54875468 54875970 54875468 ID=Msa0607880-mRNA-1:three_prime_utr;Parent=Msa0607880-mRNA-1
Gene Sequence

>Msa0607880

ATGTTGTTTATAGATGGCGAAACGAGCCCAAGGCAGGTGGTGGGAATGGCTTACATGTGCACTGACAGTGGAAACCTAATGGCAATAGCTCAACAAGTCATCAACCAAAAACAACAACAAGAACAACATGAACAACAACAACACCAACAACATCATCATCACCAAAACATCCTAGGTATCACAAACCCTCTCTCTGTCTCACTACATCCATGGCACAACAACAACAACGTTCCAGTATCTTCTTCTACATCACTACCACCTTTGTCTTTTCCACCTACCGATTTCACCGACCCATTTCAAGTCGGGTCGGGTCCTGAAAACACCGATCCCGCTTTCAGCTTCCCTTCACCACTCGACCCTCACTCAACCACCTTCCGGTTCTCCGATTTCGACTCCGACGACTGGATGGATACCTTAATGTCCGCCGCCGATAGCCACTCCGATTTCCATCTCAACCCGTTTTCATCATGCCCGACCCGACTCTCACCACAACCGGATCTTCTCTTACCCGCCACCGTACCGCTTCAAATCCCAATCCAACCACCCACTCCACAACGCAAAAACGACACTAACACCACCGAAACGACATCGTCGTTTTCATCTTCCACAAACCCACTTCTTAAAACCCTAACCGAAATTGCTTCACTCATCGAAACCCAAAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGTAAATCGATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCCTAACCAACAAAATCACCTCACAATCTTCAATAGCTTCTTCAAATTCTTCTTCAACTACTTGGGAAGAGTTAACACTCTCTTACAAAGCCTTAAACGATGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCAATTCTTGAAGCAACAGAAGGATCCAACAATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCGCCAGTTTCATCGATTTCAGCTACTGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTTACGCCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAGCCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCCGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATATGAAGCTAGTTTGACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAACCGAACATGGCGTTGGATTCGCCGGAGAGGTTTCAGGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGCTGTGGTTTTGAATCAGTGGGTTTGAGTCATTATGCAATTAGTCAAGCTAAGATATTGTTGTGGAATTATAGTTATAGTTCTTTGTATTCTCTTGTGGAATCTCAACCTGCTTTTTTGTCTCTGGCTTGGAAAGATGTTCCTCTATTGACTGTTTCTTCTTGGCGTTGA
Protein sequence

>Msa0607880

MLFIDGETSPRQVVGMAYMCTDSGNLMAIAQQVINQKQQQEQHEQQQHQQHHHHQNILGITNPLSVSLHPWHNNNNVPVSSSTSLPPLSFPPTDFTDPFQVGSGPENTDPAFSFPSPLDPHSTTFRFSDFDSDDWMDTLMSAADSHSDFHLNPFSSCPTRLSPQPDLLLPATVPLQIPIQPPTPQRKNDTNTTETTSSFSSSTNPLLKTLTEIASLIETQKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITSQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNNIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGFESVGLSHYAISQAKILLWNYSYSSLYSLVESQPAFLSLAWKDVPLLTVSSWR*