AlfalfaGEDB | Msa0608800

M. sativa cultivar zhongmu-4 / Msa0608800

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0608800	AES89785.2	91.667	312	17	2	1	309	1	306	0.0	513

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0608800	sp\|Q8VZI9\|ENAP1_ARATH	40.614	293	120	7	13	305	9	247	4.62e-48	164

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0608800	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0608800	MtrunA17_Chr4g0041151	92.532	308	14	2	1	305	1	302	0.0	510
Msa0608800	MtrunA17_Chr2g0283871	51.768	311	120	7	19	306	11	314	4.81e-86	261
Msa0608800	MtrunA17_Chr8g0344681	38.567	293	144	7	27	300	64	339	3.45e-45	157
Msa0608800	MtrunA17_Chr2g0325841	33.671	395	160	11	8	308	23	409	3.41e-39	147
Msa0608800	MtrunA17_Chr2g0331931	30.464	302	147	8	2	296	248	493	4.74e-27	110
Msa0608800	MtrunA17_Chr1g0179531	36.634	101	59	2	31	131	133	228	1.14e-16	80.5
Msa0608800	MtrunA17_Chr6g0485241	37.895	95	53	2	31	124	130	219	4.88e-16	77.8
Msa0608800	MtrunA17_Chr3g0082261	25.175	286	178	9	26	294	33	299	8.26e-15	73.9
Msa0608800	MtrunA17_Chr4g0066271	35.922	103	63	2	30	131	116	216	2.28e-14	73.2
Msa0608800	MtrunA17_Chr1g0164461	33.333	102	60	2	26	127	33	126	4.00e-12	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0608800	AT3G58630.2	46.933	326	124	9	1	302	1	301	2.75e-73	228
Msa0608800	AT3G58630.1	46.933	326	124	9	1	302	1	301	3.52e-73	229
Msa0608800	AT3G11100.1	40.614	293	120	7	13	305	9	247	4.70e-49	164
Msa0608800	AT5G05550.3	40.143	279	113	6	27	305	20	244	3.57e-48	162
Msa0608800	AT5G05550.1	40.143	279	113	6	27	305	20	244	3.88e-48	162
Msa0608800	AT5G05550.2	40.288	278	112	6	27	304	20	243	4.70e-48	162
Msa0608800	AT3G14180.1	59.615	104	38	2	27	130	80	179	2.86e-34	130
Msa0608800	AT1G54060.1	51.485	101	44	2	27	127	90	185	7.09e-22	95.1
Msa0608800	AT3G24490.1	27.925	265	139	6	31	295	90	302	1.55e-21	93.6
Msa0608800	AT3G10030.2	39.669	121	66	3	16	134	144	259	1.56e-20	92.4

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Find 108 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGCACTAAACGTGCTTC+TGG	0.443118	4_2:-56071082	Msa0608800:CDS
AAAGAGACGAAGCATCGTTC+CGG	0.461522	4_2:-56071399	Msa0608800:CDS
AAAGTGTGGAGTCAGAGGAA+TGG	0.557513	4_2:-56071327	Msa0608800:CDS
AAGAAAGGAAGAGAAAACGA+TGG	0.648071	4_2:-56071298	Msa0608800:CDS
ACAGAGACAAATGGTGGAAT+TGG	0.329589	4_2:-56071191	Msa0608800:CDS
ACCCCAGGCGTCGATGAGGG+TGG	0.576167	4_2:+56071855	None:intergenic
ACCGTCTCCACCGTCGCCTC+CGG	0.494561	4_2:-56071485	Msa0608800:CDS
ACGGCGGTTACCAGAGTCCC+TGG	0.554940	4_2:-56071645	Msa0608800:CDS
ACGGGGAACGGCGATGACGA+CGG	0.590204	4_2:+56071910	None:intergenic
ACGTCGCGCTTTCTTGTTAC+CGG	0.397117	4_2:+56071747	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTGAGTTTGGAAAATCGT+CGG	+	chr4_2:56070993-56071012	None:intergenic	35.0%
AAGAAAGGAAGAGAAAACGA+TGG	-	chr4_2:56071689-56071708	Msa0608800:CDS	35.0%
AGAAAGGAAGAGAAAACGAT+GGG	-	chr4_2:56071690-56071709	Msa0608800:CDS	35.0%
AGCTTCAAAACAGAGACAAA+TGG	-	chr4_2:56071787-56071806	Msa0608800:CDS	35.0%
TTCAAAACAGAGACAAATGG+TGG	-	chr4_2:56071790-56071809	Msa0608800:CDS	35.0%
TCAGAGAATGCAGTTATACA+TGG	-	chr4_2:56071856-56071875	Msa0608800:CDS	35.0%
TGTTGAGTTTGGAAAATCGT+CGG	+	chr4_2:56070993-56071012	None:intergenic	35.0%
AAGAAAGGAAGAGAAAACGA+TGG	-	chr4_2:56071689-56071708	Msa0608800:CDS	35.0%
AGAAAGGAAGAGAAAACGAT+GGG	-	chr4_2:56071690-56071709	Msa0608800:CDS	35.0%
AGCTTCAAAACAGAGACAAA+TGG	-	chr4_2:56071787-56071806	Msa0608800:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	56070894	56072115	56070894	ID=Msa0608800;Name=Msa0608800
chr4_2	mRNA	56070894	56072115	56070894	ID=Msa0608800-mRNA-1;Parent=Msa0608800;Name=Msa0608800-mRNA-1;_AED=0.14;_eAED=0.14;_QI=129\|0\|0.5\|0.5\|1\|1\|2\|0\|316
chr4_2	exon	56070894	56070934	56070894	ID=Msa0608800-mRNA-1:exon:14384;Parent=Msa0608800-mRNA-1
chr4_2	exon	56071077	56072115	56071077	ID=Msa0608800-mRNA-1:exon:14383;Parent=Msa0608800-mRNA-1
chr4_2	five_prime_UTR	56071987	56072115	56071987	ID=Msa0608800-mRNA-1:five_prime_utr;Parent=Msa0608800-mRNA-1
chr4_2	CDS	56071077	56071986	56071077	ID=Msa0608800-mRNA-1:cds;Parent=Msa0608800-mRNA-1
chr4_2	CDS	56070894	56070934	56070894	ID=Msa0608800-mRNA-1:cds;Parent=Msa0608800-mRNA-1

Gene Sequence

>Msa0608800

ATGGACATCATCGCCGGAGCTCCGTCACCTATCAACTCCGTTCCGCCGCGTCTTCCGTCGTCATCGCCGTTCCCCGTCCGCGAAGACTGCTGGACCGAAGACGCAACTTCCACCCTCATCGACGCCTGGGGTGAACGCTACCTCGACCTTAACCGCGGTAACCTTCGTCAGAAAACTTGGCAAGAGGTTGCCGACGCCGTCAACGACGTCCACGCCGCCGGTAACAAGAAAGCGCGACGTACAGACGTTCAGTGCAAAAACCGAATCGACACGTTAAAGAAGAAGTACAAGATCGAGAAAGCTAGGGTTTCCGATTCCGACGGCGGTTACCAGAGTCCCTGGCCGTTTTTCACTCGTCTTGACGTTCTCATCGGCGATACTTTTCCGGTCAAAAAACTCTCCCCGCCGGTGAATGTACGGTCTACTCCAACCGTCGTCAAACGTCCACCTCCTCAATCTCCACCGTCTCCACCTTCTCCACCGTCTCCACCGTCGCCTCCGGCGTGGATAATCTCTCATCCGGTCGGTCCCCGATCCGGAACACAAAAACGTCCGGCAATGGCGAAAAGAGACGAAGCATCGTTCCGGCGGAATTTCTCGGCGTTCGCGGCGGCTGCTGCGGCGGCGGCTGAGGCAGAAAGTGTGGAGTCAGAGGAATGGAGGGGGAAGAAAGGAAGAGAAAACGATGGGACCCATAAGGAGTTTGGTTTCAGAGAGCTCGCTCAGGCTATAGAGAGATTCAGTGAAGTATATGAAAGAGTTGAAGCTTCAAAACAGAGACAAATGGTGGAATTGGAGAAGCAGAGGATGCAGTTTGTGAAGGATTTGGAGTATCAGAGAATGCAGTTATACATGGAAACGCAGGTTCAGCTTCAAAAAATCAAACGCACTAAACGTGCTTCTGGTTCCGCAGAAGTTGGGTATTTTGTTACTTGTGATAATGGTAACTGA

Protein sequence

>Msa0608800

MDIIAGAPSPINSVPPRLPSSSPFPVREDCWTEDATSTLIDAWGERYLDLNRGNLRQKTWQEVADAVNDVHAAGNKKARRTDVQCKNRIDTLKKKYKIEKARVSDSDGGYQSPWPFFTRLDVLIGDTFPVKKLSPPVNVRSTPTVVKRPPPQSPPSPPSPPSPPSPPAWIISHPVGPRSGTQKRPAMAKRDEASFRRNFSAFAAAAAAAAEAESVESEEWRGKKGRENDGTHKEFGFRELAQAIERFSEVYERVEASKQRQMVELEKQRMQFVKDLEYQRMQLYMETQVQLQKIKRTKRASGSAEVGYFVTCDNGN*