AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0608870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608870 MtrunA17_Chr4g0041301 100.000 142 0 0 1 142 1 142 5.50e-101 285
Msa0608870 MtrunA17_Chr2g0320211 91.304 138 12 0 5 142 3 140 2.67e-89 256
Msa0608870 MtrunA17_Chr6g0485281 50.435 115 57 0 4 118 9 123 5.81e-34 115
Msa0608870 MtrunA17_Chr6g0457491 87.273 55 7 0 88 142 1 55 7.87e-28 97.8
Msa0608870 MtrunA17_Chr7g0275831 90.000 40 4 0 92 131 1 40 5.29e-19 75.5
Msa0608870 MtrunA17_Chr4g0057871 55.844 77 21 1 32 95 9 85 2.33e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608870 AT3G58680.1 84.507 142 22 0 1 142 1 142 1.32e-86 249
Msa0608870 AT2G42680.1 85.915 142 20 0 1 142 1 142 1.77e-86 249
Msa0608870 AT3G24500.1 50.360 139 67 1 5 141 7 145 1.09e-43 141
Msa0608870 AT3G24500.2 43.820 89 48 1 5 91 7 95 4.84e-17 71.6

Find 73 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCATTGGCCTGGTTTGC+AGG 0.172981 4_2:+56243675 Msa0608870:three_prime_UTR
ATTGGTGTTCAAGAATTAAT+TGG 0.217200 4_2:-56243702 None:intergenic
GACGGCTTTCTCATCTTTCT+TGG 0.250063 4_2:-56241209 None:intergenic
ATCTTTCTTGGCGGCGGCGT+TGG 0.250269 4_2:-56241197 None:intergenic
TTGCTAATTATCTGCTGGTT+TGG 0.292459 4_2:-56243381 None:intergenic
GTTCCCAATCTTGAGAAATA+TGG 0.320777 4_2:-56241150 None:intergenic
CTGTGTTTATCTTGGTTCAT+TGG 0.327924 4_2:+56243662 Msa0608870:three_prime_UTR
TAGCTCAGTGAAGGTGTAAA+TGG 0.330318 4_2:-56241061 None:intergenic
CCATCACTCTGGCTGTTAGA+AGG 0.335469 4_2:+56243859 Msa0608870:three_prime_UTR
TTTCTTGACGGTATCGATAT+CGG 0.338092 4_2:-56241254 None:intergenic
TCTTTCTTGGCGGCGGCGTT+GGG 0.340591 4_2:-56241196 None:intergenic
GGCACGGCGAGCGGCGTTGA+CGG 0.351560 4_2:-56241227 None:intergenic
GTCAACGCCGCTCGCCGTGC+CGG 0.353656 4_2:+56241229 Msa0608870:CDS
GGGTTTGCATGTCACTCTTA+AGG 0.362036 4_2:+56243472 Msa0608870:three_prime_UTR
GTATATGTAAAACATATAAA+TGG 0.389403 4_2:+56243527 Msa0608870:three_prime_UTR
TGACAAGTACTCTGCAAATC+AGG 0.389884 4_2:-56243766 None:intergenic
TAGGCCATATTTCTCAAGAT+TGG 0.393130 4_2:+56241146 Msa0608870:CDS
AGCAAGTTGGAGAGAGCCCT+TGG 0.404653 4_2:+56243399 Msa0608870:CDS
GTTCATTGGCCTGGTTTGCA+GGG 0.414765 4_2:+56243676 Msa0608870:three_prime_UTR
TTGGTGTTCAAGAATTAATT+GGG 0.418275 4_2:-56243701 None:intergenic
CCAACTTGCTAATTATCTGC+TGG 0.425113 4_2:-56243386 None:intergenic
GTGATCCAAGAGTACGAGTC+AGG 0.425391 4_2:+56243348 Msa0608870:CDS
CTTTCCCTGACTCGTACTCT+TGG 0.428066 4_2:-56243353 None:intergenic
AAGTTACAAATTTCAAACTT+AGG 0.430321 4_2:-56243736 None:intergenic
AGGCCATATTTCTCAAGATT+GGG 0.431239 4_2:+56241147 Msa0608870:CDS
ATTTCGCTTATTCGAACAAC+AGG 0.432195 4_2:-56243569 None:intergenic
TTCTTGAACACCAATGGACT+TGG 0.442586 4_2:+56243710 Msa0608870:three_prime_UTR
ATCGGCGCCGGCACGGCGAG+CGG 0.444792 4_2:-56241236 None:intergenic
GCTTTCTTGCGGATGACGAC+TGG 0.447489 4_2:-56241172 None:intergenic
CTGATTTCATGATTGTGCGC+TGG 0.453008 4_2:+56243451 Msa0608870:three_prime_UTR
AGTGAGTGCTGTGTTTATCT+TGG 0.458752 4_2:+56243654 Msa0608870:three_prime_UTR
CATCACTCTGGCTGTTAGAA+GGG 0.473730 4_2:+56243860 Msa0608870:three_prime_UTR
AATTATCTGCTGGTTTGGAA+TGG 0.474147 4_2:-56243376 None:intergenic
GAGATAAACATGTCAGGTCT+AGG 0.474885 4_2:+56241127 Msa0608870:exon
CAGCGCACAATCATGAAATC+AGG 0.477136 4_2:-56243450 None:intergenic
TTTATCTTGGTTCATTGGCC+TGG 0.479258 4_2:+56243667 Msa0608870:three_prime_UTR
TGATTTCATGATTGTGCGCT+GGG 0.486261 4_2:+56243452 Msa0608870:three_prime_UTR
GCACTTCATTGAACACTAAG+AGG 0.487095 4_2:+56241595 Msa0608870:CDS
GTGATGGTGAAGGTGGTTCA+TGG 0.487129 4_2:-56243843 None:intergenic
AATTGGGTCCCCTGCAAACC+AGG 0.492034 4_2:-56243685 None:intergenic
GGCTATAATGCAAGCTAGGA+TGG 0.493126 4_2:+56243186 Msa0608870:CDS
TTGAAAATCACTTGAAAGGA+GGG 0.498570 4_2:-56243609 None:intergenic
AATTAATTCTTGAACACCAA+TGG 0.503254 4_2:+56243704 Msa0608870:three_prime_UTR
TTCATTGAACACTAAGAGGC+TGG 0.503842 4_2:+56241599 Msa0608870:CDS
TGCCACGCAGTTTAGCTCCA+AGG 0.505877 4_2:-56243416 None:intergenic
CCAGCAGATAATTAGCAAGT+TGG 0.509376 4_2:+56243386 Msa0608870:CDS
AACAAAGAGATAAACATGTC+AGG 0.512918 4_2:+56241121 Msa0608870:exon
ATAGCCTTCTTGAGTTCAGT+TGG 0.514235 4_2:-56243169 None:intergenic
AGAAGGCTATAATGCAAGCT+AGG 0.515044 4_2:+56243182 Msa0608870:CDS
TTCATTGGCCTGGTTTGCAG+GGG 0.520676 4_2:+56243677 Msa0608870:three_prime_UTR
GAGGAGCGAACGTTTCTTGA+CGG 0.526730 4_2:-56241266 None:intergenic
CGGCGTTGGGAGCTTTCTTG+CGG 0.528475 4_2:-56241183 None:intergenic
GCCACGCAGTTTAGCTCCAA+GGG 0.531362 4_2:-56243415 None:intergenic
GGCTTTCTCATCTTTCTTGG+CGG 0.532271 4_2:-56241206 None:intergenic
ACTTAGGATTCCAAGTCCAT+TGG 0.537779 4_2:-56243720 None:intergenic
CTTGAAAATCACTTGAAAGG+AGG 0.538825 4_2:-56243610 None:intergenic
TACCTTGAAAATCACTTGAA+AGG 0.542425 4_2:-56243613 None:intergenic
GAACACTAAGAGGCTGGATG+AGG 0.556069 4_2:+56241605 Msa0608870:CDS
GACGGTATCGATATCGGCGC+CGG 0.571328 4_2:-56241248 None:intergenic
ATCACTCTGGCTGTTAGAAG+GGG 0.579964 4_2:+56243861 Msa0608870:three_prime_UTR
TAGGGTTCGTAGCTCAGTGA+AGG 0.583142 4_2:-56241070 None:intergenic
TTTCTCATCTTTCTTGGCGG+CGG 0.585231 4_2:-56241203 None:intergenic
TGTACCAACTGAACTCAAGA+AGG 0.594355 4_2:+56243165 Msa0608870:CDS
TCGTACTCTTGGATCACTTG+AGG 0.598699 4_2:-56243342 None:intergenic
AACCACCTTCACCATCACTC+TGG 0.601888 4_2:+56243848 Msa0608870:three_prime_UTR
AGCCAGAGTGATGGTGAAGG+TGG 0.604553 4_2:-56243850 None:intergenic
TGATCCAAGAGTACGAGTCA+GGG 0.620671 4_2:+56243349 Msa0608870:CDS
TATCGATATCGGCGCCGGCA+CGG 0.624694 4_2:-56241243 None:intergenic
CCTTCTAACAGCCAGAGTGA+TGG 0.626333 4_2:-56243859 None:intergenic
CGAACAACAGGTGAAAATCA+CGG 0.647680 4_2:-56243557 None:intergenic
GCCCTTGGAGCTAAACTGCG+TGG 0.653713 4_2:+56243414 Msa0608870:CDS
AACAGCCAGAGTGATGGTGA+AGG 0.676146 4_2:-56243853 None:intergenic
TCACTCTGGCTGTTAGAAGG+GGG 0.697930 4_2:+56243862 Msa0608870:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTAATTAATTAGTTTT+GGG - chr4_2:56241294-56241313 None:intergenic 10.0%
!!! AAACATTAATTAATTAGTTT+TGG - chr4_2:56241295-56241314 None:intergenic 10.0%
!!! TTAATGTTTTTTTTAACTCT+TGG + chr4_2:56241306-56241325 Msa0608870:intron 15.0%
!!! TTCGTAGATAAAAATAATTT+TGG + chr4_2:56241419-56241438 Msa0608870:intron 15.0%
!!! TTTGATGATTATTTTGTATT+AGG + chr4_2:56241443-56241462 Msa0608870:intron 15.0%
!!! TTGATGATTATTTTGTATTA+GGG + chr4_2:56241444-56241463 Msa0608870:intron 15.0%
!! TAATTTCAATATATTGATCA+TGG + chr4_2:56241484-56241503 Msa0608870:intron 15.0%
!! TTCAAATTATATAATCTGAT+GGG - chr4_2:56242637-56242656 None:intergenic 15.0%
!! TTTCAAATTATATAATCTGA+TGG - chr4_2:56242638-56242657 None:intergenic 15.0%
!! GTATATGTAAAACATATAAA+TGG + chr4_2:56243527-56243546 Msa0608870:three_prime_UTR 15.0%
!!! TTTTTGAATGAGTAGTTATA+TGG + chr4_2:56241738-56241757 Msa0608870:intron 20.0%
!!! TTTTGAATGAGTAGTTATAT+GGG + chr4_2:56241739-56241758 Msa0608870:intron 20.0%
!!! TTCATATAGAAAATTTTGTG+TGG + chr4_2:56241814-56241833 Msa0608870:intron 20.0%
!!! ATTTTGAATTCATGATGTTT+TGG + chr4_2:56241997-56242016 Msa0608870:intron 20.0%
!! CAGAAAAGAAGAAAAAAAAA+TGG - chr4_2:56242218-56242237 None:intergenic 20.0%
!! AAGGAGAAATAAAAAATAAC+TGG + chr4_2:56242361-56242380 Msa0608870:intron 20.0%
!! AGGAGAAATAAAAAATAACT+GGG + chr4_2:56242362-56242381 Msa0608870:intron 20.0%
!! TCAAATTATATAATCTGATG+GGG - chr4_2:56242636-56242655 None:intergenic 20.0%
!! AACCCAATACTTATAAATTA+CGG - chr4_2:56242822-56242841 None:intergenic 20.0%
!! CATTTCCCTAAAAAATATAA+TGG - chr4_2:56243076-56243095 None:intergenic 20.0%
!! AAGTTACAAATTTCAAACTT+AGG - chr4_2:56243739-56243758 None:intergenic 20.0%
!!! AATTAATTAGTTTTGGGAAG+AGG - chr4_2:56241288-56241307 None:intergenic 25.0%
! TTTGAATGAGTAGTTATATG+GGG + chr4_2:56241740-56241759 Msa0608870:intron 25.0%
! ATATGATTCAAACTCAATCA+CGG + chr4_2:56242188-56242207 Msa0608870:intron 25.0%
! TAGATTTCGAAATCAAATTG+TGG + chr4_2:56242286-56242305 Msa0608870:intron 25.0%
! AGATTTCGAAATCAAATTGT+GGG + chr4_2:56242287-56242306 Msa0608870:intron 25.0%
! ATTAAGTAAATGCTAAACAG+TGG - chr4_2:56242430-56242449 None:intergenic 25.0%
! AGCATTTACTTAATTGTTTG+TGG + chr4_2:56242436-56242455 Msa0608870:intron 25.0%
! GCATTTACTTAATTGTTTGT+GGG + chr4_2:56242437-56242456 Msa0608870:intron 25.0%
! CATTTACTTAATTGTTTGTG+GGG + chr4_2:56242438-56242457 Msa0608870:intron 25.0%
! TCTCCGTAATTTATAAGTAT+TGG + chr4_2:56242816-56242835 Msa0608870:intron 25.0%
! CTCCGTAATTTATAAGTATT+GGG + chr4_2:56242817-56242836 Msa0608870:intron 25.0%
!! TCATGATCAAAATTTTCTCT+TGG + chr4_2:56242905-56242924 Msa0608870:intron 25.0%
! ATATCATGCTTAATAGATAG+AGG + chr4_2:56243039-56243058 Msa0608870:intron 25.0%
!!! TGTCCACCATTATATTTTTT+AGG + chr4_2:56243067-56243086 Msa0608870:intron 25.0%
!!! GTCCACCATTATATTTTTTA+GGG + chr4_2:56243068-56243087 Msa0608870:intron 25.0%
! TTCCCTAAAAAATATAATGG+TGG - chr4_2:56243073-56243092 None:intergenic 25.0%
! CATGTTATCAATAACAAACA+TGG + chr4_2:56243294-56243313 Msa0608870:intron 25.0%
!! TTGGTGTTCAAGAATTAATT+GGG - chr4_2:56243704-56243723 None:intergenic 25.0%
!! ATTGGTGTTCAAGAATTAAT+TGG - chr4_2:56243705-56243724 None:intergenic 25.0%
! AATTAATTCTTGAACACCAA+TGG + chr4_2:56243704-56243723 Msa0608870:three_prime_UTR 25.0%
! TGTTTTTCTGTGTCTGTTTA+GGG - chr4_2:56241091-56241110 None:intergenic 30.0%
AACAAAGAGATAAACATGTC+AGG + chr4_2:56241121-56241140 Msa0608870:exon 30.0%
TATGAAAATACTTAAGCTGC+AGG - chr4_2:56241800-56241819 None:intergenic 30.0%
! AAAATTTTGTGTGGAACATG+TGG + chr4_2:56241823-56241842 Msa0608870:intron 30.0%
TTCTACAGCATAAATTTGAG+CGG - chr4_2:56242051-56242070 None:intergenic 30.0%
!!! AACATATTTTGAGTTGTGCT+TGG - chr4_2:56242093-56242112 None:intergenic 30.0%
CAAAATATGTTTGCATAGCT+TGG + chr4_2:56242102-56242121 Msa0608870:intron 30.0%
CAGAAAACAGGACTAAAATA+TGG + chr4_2:56242327-56242346 Msa0608870:intron 30.0%
! AATTATAGGGTGGATTTGAA+TGG + chr4_2:56242462-56242481 Msa0608870:intron 30.0%
! AAATTTTCTCTTGGCTTCAT+TGG + chr4_2:56242914-56242933 Msa0608870:intron 30.0%
AATAAAGCAACTGAACAATC+AGG - chr4_2:56242957-56242976 None:intergenic 30.0%
TAAAGAACATAAAGAGACTG+AGG - chr4_2:56243264-56243283 None:intergenic 30.0%
TTGAAAATCACTTGAAAGGA+GGG - chr4_2:56243612-56243631 None:intergenic 30.0%
TACCTTGAAAATCACTTGAA+AGG - chr4_2:56243616-56243635 None:intergenic 30.0%
!!! AACCTTGTTTTTTCGACAAA+TGG - chr4_2:56243816-56243835 None:intergenic 30.0%
! GTGTTTTTCTGTGTCTGTTT+AGG - chr4_2:56241092-56241111 None:intergenic 35.0%
TAGGCCATATTTCTCAAGAT+TGG + chr4_2:56241146-56241165 Msa0608870:CDS 35.0%
AGGCCATATTTCTCAAGATT+GGG + chr4_2:56241147-56241166 Msa0608870:CDS 35.0%
GTTCCCAATCTTGAGAAATA+TGG - chr4_2:56241153-56241172 None:intergenic 35.0%
!! TTTCTTGACGGTATCGATAT+CGG - chr4_2:56241257-56241276 None:intergenic 35.0%
! GGTTGTGTGTTTTGTGATTT+TGG + chr4_2:56241505-56241524 Msa0608870:intron 35.0%
! GTGTGTTTTGTGATTTTGGA+TGG + chr4_2:56241509-56241528 Msa0608870:intron 35.0%
! TGATTTTGGATGGTGATTGT+TGG + chr4_2:56241519-56241538 Msa0608870:intron 35.0%
CCATTGATAGAATAGAACCT+CGG + chr4_2:56241709-56241728 Msa0608870:intron 35.0%
!! CTTTAAGCACTGCTAACTTT+TGG + chr4_2:56241937-56241956 Msa0608870:intron 35.0%
GCTTGGACTAAAAATCAACA+TGG - chr4_2:56242076-56242095 None:intergenic 35.0%
! AAATATGGCTTGTACACTCA+AGG + chr4_2:56242342-56242361 Msa0608870:intron 35.0%
ATTGTTTGTGGGGCAATTAT+AGG + chr4_2:56242448-56242467 Msa0608870:intron 35.0%
TTGTTTGTGGGGCAATTATA+GGG + chr4_2:56242449-56242468 Msa0608870:intron 35.0%
! CATTGAAACTGTTTTCGCAA+AGG + chr4_2:56242516-56242535 Msa0608870:intron 35.0%
ACTGTCCTTGTAAAGTTGAA+TGG + chr4_2:56242752-56242771 Msa0608870:intron 35.0%
GCTAACCATTCAACTTTACA+AGG - chr4_2:56242760-56242779 None:intergenic 35.0%
AGTGACAACAAAGACGTTAA+AGG - chr4_2:56242790-56242809 None:intergenic 35.0%
ATCTCATCAAGCATGAGTTA+AGG - chr4_2:56242850-56242869 None:intergenic 35.0%
! AGGGAAATGTTAACCACTTT+TGG + chr4_2:56243087-56243106 Msa0608870:intron 35.0%
AACATAAAGAGACTGAGGTT+GGG - chr4_2:56243259-56243278 None:intergenic 35.0%
!! AATTATCTGCTGGTTTGGAA+TGG - chr4_2:56243379-56243398 None:intergenic 35.0%
! TTGCTAATTATCTGCTGGTT+TGG - chr4_2:56243384-56243403 None:intergenic 35.0%
ATTTCGCTTATTCGAACAAC+AGG - chr4_2:56243572-56243591 None:intergenic 35.0%
CTTGAAAATCACTTGAAAGG+AGG - chr4_2:56243613-56243632 None:intergenic 35.0%
!! CTCCTTTCAAGTGATTTTCA+AGG + chr4_2:56243611-56243630 Msa0608870:three_prime_UTR 35.0%
CTGTGTTTATCTTGGTTCAT+TGG + chr4_2:56243662-56243681 Msa0608870:three_prime_UTR 35.0%
CTCCATTTGTCGAAAAAACA+AGG + chr4_2:56243811-56243830 Msa0608870:three_prime_UTR 35.0%
! TAGCTCAGTGAAGGTGTAAA+TGG - chr4_2:56241064-56241083 None:intergenic 40.0%
GAGATAAACATGTCAGGTCT+AGG + chr4_2:56241127-56241146 Msa0608870:exon 40.0%
CGTATTGCGATTGTTGTAGT+TGG + chr4_2:56241336-56241355 Msa0608870:intron 40.0%
GCACTTCATTGAACACTAAG+AGG + chr4_2:56241595-56241614 Msa0608870:CDS 40.0%
TTCATTGAACACTAAGAGGC+TGG + chr4_2:56241599-56241618 Msa0608870:CDS 40.0%
CCGAGGTTCTATTCTATCAA+TGG - chr4_2:56241712-56241731 None:intergenic 40.0%
TCATTCAAAAATCGACACCG+AGG - chr4_2:56241729-56241748 None:intergenic 40.0%
GTTATATGGGGAGAATGATG+AGG + chr4_2:56241752-56241771 Msa0608870:intron 40.0%
TTTGTGTGGAACATGTGGTT+TGG + chr4_2:56241828-56241847 Msa0608870:intron 40.0%
ATGTTTGCATAGCTTGGACA+TGG + chr4_2:56242108-56242127 Msa0608870:intron 40.0%
TGTTTGCATAGCTTGGACAT+GGG + chr4_2:56242109-56242128 Msa0608870:intron 40.0%
TCAAATTGTGGGTCAAGTTC+CGG + chr4_2:56242298-56242317 Msa0608870:intron 40.0%
TGTATTGGAAGTCCATCTAG+CGG + chr4_2:56242559-56242578 Msa0608870:intron 40.0%
AGAGAGAGCGATCACATAAT+GGG - chr4_2:56242671-56242690 None:intergenic 40.0%
! TCTTGCTGTGTGTGTAATTG+TGG + chr4_2:56242694-56242713 Msa0608870:intron 40.0%
!! ACACAGCATGCTTTGGAATT+TGG + chr4_2:56242997-56243016 Msa0608870:intron 40.0%
GATGTGCATGAATCCAAAAG+TGG - chr4_2:56243103-56243122 None:intergenic 40.0%
TCTGTTGACAATCACATGAC+TGG + chr4_2:56243121-56243140 Msa0608870:intron 40.0%
TGTACCAACTGAACTCAAGA+AGG + chr4_2:56243165-56243184 Msa0608870:CDS 40.0%
ATAGCCTTCTTGAGTTCAGT+TGG - chr4_2:56243172-56243191 None:intergenic 40.0%
AGAAGGCTATAATGCAAGCT+AGG + chr4_2:56243182-56243201 Msa0608870:CDS 40.0%
ACATAAAGAGACTGAGGTTG+GGG - chr4_2:56243258-56243277 None:intergenic 40.0%
GAACATAAAGAGACTGAGGT+TGG - chr4_2:56243260-56243279 None:intergenic 40.0%
!! CCAACTTGCTAATTATCTGC+TGG - chr4_2:56243389-56243408 None:intergenic 40.0%
CCAGCAGATAATTAGCAAGT+TGG + chr4_2:56243386-56243405 Msa0608870:CDS 40.0%
TGATTTCATGATTGTGCGCT+GGG + chr4_2:56243452-56243471 Msa0608870:three_prime_UTR 40.0%
CGAACAACAGGTGAAAATCA+CGG - chr4_2:56243560-56243579 None:intergenic 40.0%
! AGTGAGTGCTGTGTTTATCT+TGG + chr4_2:56243654-56243673 Msa0608870:three_prime_UTR 40.0%
TTTATCTTGGTTCATTGGCC+TGG + chr4_2:56243667-56243686 Msa0608870:three_prime_UTR 40.0%
! TTCTTGAACACCAATGGACT+TGG + chr4_2:56243710-56243729 Msa0608870:three_prime_UTR 40.0%
ACTTAGGATTCCAAGTCCAT+TGG - chr4_2:56243723-56243742 None:intergenic 40.0%
TGACAAGTACTCTGCAAATC+AGG - chr4_2:56243769-56243788 None:intergenic 40.0%
TTTCTCATCTTTCTTGGCGG+CGG - chr4_2:56241206-56241225 None:intergenic 45.0%
GGCTTTCTCATCTTTCTTGG+CGG - chr4_2:56241209-56241228 None:intergenic 45.0%
GACGGCTTTCTCATCTTTCT+TGG - chr4_2:56241212-56241231 None:intergenic 45.0%
!! TGTCTACTGACTCTTGCAAC+TGG + chr4_2:56241863-56241882 Msa0608870:intron 45.0%
TTGGTCCAATGTCCTCTAGT+AGG + chr4_2:56242141-56242160 Msa0608870:intron 45.0%
CGTTACCTACTAGAGGACAT+TGG - chr4_2:56242149-56242168 None:intergenic 45.0%
AGTCTGTCGTTACCTACTAG+AGG - chr4_2:56242156-56242175 None:intergenic 45.0%
TTCCGGATCATGCAGAAAAC+AGG + chr4_2:56242315-56242334 Msa0608870:intron 45.0%
! GTCCTGTTTTCTGCATGATC+CGG - chr4_2:56242320-56242339 None:intergenic 45.0%
TTTGTGGGGCAATTATAGGG+TGG + chr4_2:56242452-56242471 Msa0608870:intron 45.0%
! GTTTTCGCAAAGGAAAGCCT+TGG + chr4_2:56242526-56242545 Msa0608870:intron 45.0%
!! AAAGGAAAGCCTTGGCTCTA+AGG + chr4_2:56242534-56242553 Msa0608870:intron 45.0%
CAATACAAGCCTTAGAGCCA+AGG - chr4_2:56242546-56242565 None:intergenic 45.0%
!! CTTGGCTCTAAGGCTTGTAT+TGG + chr4_2:56242544-56242563 Msa0608870:intron 45.0%
CAATGCATGAATCCGCTAGA+TGG - chr4_2:56242574-56242593 None:intergenic 45.0%
GAGAGAGCGATCACATAATG+GGG - chr4_2:56242670-56242689 None:intergenic 45.0%
GAGAGAGAGCGATCACATAA+TGG - chr4_2:56242672-56242691 None:intergenic 45.0%
ATTGTGCACACAGCATGCTT+TGG + chr4_2:56242990-56243009 Msa0608870:intron 45.0%
GGCTATAATGCAAGCTAGGA+TGG + chr4_2:56243186-56243205 Msa0608870:CDS 45.0%
ATAAAGAGACTGAGGTTGGG+GGG - chr4_2:56243256-56243275 None:intergenic 45.0%
CATAAAGAGACTGAGGTTGG+GGG - chr4_2:56243257-56243276 None:intergenic 45.0%
TCGTACTCTTGGATCACTTG+AGG - chr4_2:56243345-56243364 None:intergenic 45.0%
! TGATCCAAGAGTACGAGTCA+GGG + chr4_2:56243349-56243368 Msa0608870:CDS 45.0%
CAGCGCACAATCATGAAATC+AGG - chr4_2:56243453-56243472 None:intergenic 45.0%
CTGATTTCATGATTGTGCGC+TGG + chr4_2:56243451-56243470 Msa0608870:three_prime_UTR 45.0%
GGGTTTGCATGTCACTCTTA+AGG + chr4_2:56243472-56243491 Msa0608870:three_prime_UTR 45.0%
CATCACTCTGGCTGTTAGAA+GGG + chr4_2:56243860-56243879 Msa0608870:three_prime_UTR 45.0%
ATCACTCTGGCTGTTAGAAG+GGG + chr4_2:56243861-56243880 Msa0608870:three_prime_UTR 45.0%
!!! TATTTTTTTTATATCTTAAT+TGG - chr4_2:56243949-56243968 None:intergenic 5.0%
TAGGGTTCGTAGCTCAGTGA+AGG - chr4_2:56241073-56241092 None:intergenic 50.0%
GAGGAGCGAACGTTTCTTGA+CGG - chr4_2:56241269-56241288 None:intergenic 50.0%
GAACACTAAGAGGCTGGATG+AGG + chr4_2:56241605-56241624 Msa0608870:CDS 50.0%
! TGGACATGGGAGTGTGATCT+TGG + chr4_2:56242122-56242141 Msa0608870:intron 50.0%
! GTGATCCAAGAGTACGAGTC+AGG + chr4_2:56243348-56243367 Msa0608870:CDS 50.0%
! CTTTCCCTGACTCGTACTCT+TGG - chr4_2:56243356-56243375 None:intergenic 50.0%
! GTTCATTGGCCTGGTTTGCA+GGG + chr4_2:56243676-56243695 Msa0608870:three_prime_UTR 50.0%
! TTCATTGGCCTGGTTTGCAG+GGG + chr4_2:56243677-56243696 Msa0608870:three_prime_UTR 50.0%
! GTGATGGTGAAGGTGGTTCA+TGG - chr4_2:56243846-56243865 None:intergenic 50.0%
AACCACCTTCACCATCACTC+TGG + chr4_2:56243848-56243867 Msa0608870:three_prime_UTR 50.0%
! AACAGCCAGAGTGATGGTGA+AGG - chr4_2:56243856-56243875 None:intergenic 50.0%
CCTTCTAACAGCCAGAGTGA+TGG - chr4_2:56243862-56243881 None:intergenic 50.0%
CCATCACTCTGGCTGTTAGA+AGG + chr4_2:56243859-56243878 Msa0608870:three_prime_UTR 50.0%
TCACTCTGGCTGTTAGAAGG+GGG + chr4_2:56243862-56243881 Msa0608870:three_prime_UTR 50.0%
GCTTTCTTGCGGATGACGAC+TGG - chr4_2:56241175-56241194 None:intergenic 55.0%
! AGCAAGTTGGAGAGAGCCCT+TGG + chr4_2:56243399-56243418 Msa0608870:CDS 55.0%
! GCCACGCAGTTTAGCTCCAA+GGG - chr4_2:56243418-56243437 None:intergenic 55.0%
! TGCCACGCAGTTTAGCTCCA+AGG - chr4_2:56243419-56243438 None:intergenic 55.0%
! GGTTCATTGGCCTGGTTTGC+AGG + chr4_2:56243675-56243694 Msa0608870:three_prime_UTR 55.0%
AATTGGGTCCCCTGCAAACC+AGG - chr4_2:56243688-56243707 None:intergenic 55.0%
! AGCCAGAGTGATGGTGAAGG+TGG - chr4_2:56243853-56243872 None:intergenic 55.0%
! CGGCGTTGGGAGCTTTCTTG+CGG - chr4_2:56241186-56241205 None:intergenic 60.0%
TCTTTCTTGGCGGCGGCGTT+GGG - chr4_2:56241199-56241218 None:intergenic 60.0%
ATCTTTCTTGGCGGCGGCGT+TGG - chr4_2:56241200-56241219 None:intergenic 60.0%
!! TATCGATATCGGCGCCGGCA+CGG - chr4_2:56241246-56241265 None:intergenic 60.0%
!! GACGGTATCGATATCGGCGC+CGG - chr4_2:56241251-56241270 None:intergenic 60.0%
GCCCTTGGAGCTAAACTGCG+TGG + chr4_2:56243414-56243433 Msa0608870:CDS 60.0%
! GGCACGGCGAGCGGCGTTGA+CGG - chr4_2:56241230-56241249 None:intergenic 75.0%
GTCAACGCCGCTCGCCGTGC+CGG + chr4_2:56241229-56241248 Msa0608870:CDS 75.0%
!!! ATCGGCGCCGGCACGGCGAG+CGG - chr4_2:56241239-56241258 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 56241052 56243977 56241052 ID=Msa0608870;Name=Msa0608870
chr4_2 mRNA 56241052 56243977 56241052 ID=Msa0608870-mRNA-1;Parent=Msa0608870;Name=Msa0608870-mRNA-1;_AED=0.04;_eAED=0.04;_QI=84|1|1|1|1|1|4|531|142
chr4_2 exon 56241052 56241277 56241052 ID=Msa0608870-mRNA-1:exon:14416;Parent=Msa0608870-mRNA-1
chr4_2 exon 56241562 56241645 56241562 ID=Msa0608870-mRNA-1:exon:14417;Parent=Msa0608870-mRNA-1
chr4_2 exon 56243158 56243240 56243158 ID=Msa0608870-mRNA-1:exon:14418;Parent=Msa0608870-mRNA-1
chr4_2 exon 56243327 56243977 56243327 ID=Msa0608870-mRNA-1:exon:14419;Parent=Msa0608870-mRNA-1
chr4_2 five_prime_UTR 56241052 56241135 56241052 ID=Msa0608870-mRNA-1:five_prime_utr;Parent=Msa0608870-mRNA-1
chr4_2 CDS 56241136 56241277 56241136 ID=Msa0608870-mRNA-1:cds;Parent=Msa0608870-mRNA-1
chr4_2 CDS 56241562 56241645 56241562 ID=Msa0608870-mRNA-1:cds;Parent=Msa0608870-mRNA-1
chr4_2 CDS 56243158 56243240 56243158 ID=Msa0608870-mRNA-1:cds;Parent=Msa0608870-mRNA-1
chr4_2 CDS 56243327 56243446 56243327 ID=Msa0608870-mRNA-1:cds;Parent=Msa0608870-mRNA-1
chr4_2 three_prime_UTR 56243447 56243977 56243447 ID=Msa0608870-mRNA-1:three_prime_utr;Parent=Msa0608870-mRNA-1
Gene Sequence

>Msa0608870

ATGTCAGGTCTAGGCCATATTTCTCAAGATTGGGAACCAGTCGTCATCCGCAAGAAAGCTCCCAACGCCGCCGCCAAGAAAGATGAGAAAGCCGTCAACGCCGCTCGCCGTGCCGGCGCCGATATCGATACCGTCAAGAAACATAATGCTGCAACAAACAAAGCTGCATCTAGCAGCACTTCATTGAACACTAAGAGGCTGGATGAGGATACTGAGAATCTAGCTCATGATCGTGTACCAACTGAACTCAAGAAGGCTATAATGCAAGCTAGGATGGACAAAAAGCTTACTCAGTCTCAGCTTGCTCAAATCATCAATGAGAAGCCTCAAGTGATCCAAGAGTACGAGTCAGGGAAAGCCATTCCAAACCAGCAGATAATTAGCAAGTTGGAGAGAGCCCTTGGAGCTAAACTGCGTGGCAAGAAATGA
Protein sequence

>Msa0608870

MSGLGHISQDWEPVVIRKKAPNAAAKKDEKAVNAARRAGADIDTVKKHNAATNKAASSSTSLNTKRLDEDTENLAHDRVPTELKKAIMQARMDKKLTQSQLAQIINEKPQVIQEYESGKAIPNQQIISKLERALGAKLRGKK*