AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0610820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610820 MtrunA17_Chr4g0043891 92.800 125 2 2 32 156 1 118 5.96e-78 232
Msa0610820 MtrunA17_Chr8g0392991 60.345 116 35 3 37 152 94 198 7.48e-37 130
Msa0610820 MtrunA17_Chr7g0238431 71.429 70 20 0 32 101 1 70 3.42e-31 111
Msa0610820 MtrunA17_Chr5g0436661 39.552 134 71 2 39 162 33 166 2.34e-27 101
Msa0610820 MtrunA17_Chr5g0405351 67.164 67 21 1 35 100 26 92 3.36e-27 103
Msa0610820 MtrunA17_Chr1g0153171 68.750 64 20 0 38 101 89 152 1.77e-26 100
Msa0610820 MtrunA17_Chr8g0385441 84.615 52 8 0 50 101 93 144 2.25e-26 98.2
Msa0610820 MtrunA17_Chr3g0125111 84.615 52 8 0 50 101 91 142 4.08e-26 97.4
Msa0610820 MtrunA17_Chr4g0031341 66.667 63 21 0 39 101 25 87 7.68e-26 99.0
Msa0610820 MtrunA17_Chr7g0223471 47.761 67 35 0 34 100 42 108 1.48e-18 81.3
Msa0610820 MtrunA17_Chr1g0212511 46.341 82 38 1 39 120 85 160 3.30e-18 79.0
Msa0610820 MtrunA17_Chr2g0283141 44.928 69 38 0 34 102 50 118 6.19e-18 79.7
Msa0610820 MtrunA17_Chr3g0144021 42.623 61 35 0 39 99 82 142 7.96e-14 66.2
Msa0610820 MtrunA17_Chr6g0460691 38.961 77 47 0 38 114 31 107 4.33e-13 65.5
Msa0610820 MtrunA17_Chr7g0222981 36.559 93 49 1 39 131 68 150 5.24e-13 63.5
Msa0610820 MtrunA17_Chr7g0254251 45.098 51 28 0 38 88 27 77 9.25e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610820 AT3G18010.1 53.659 123 45 2 37 156 74 187 9.51e-36 127
Msa0610820 AT2G28610.1 65.714 70 24 0 32 101 1 70 3.22e-29 107
Msa0610820 AT5G59340.1 64.706 68 24 0 37 104 12 79 1.69e-27 103
Msa0610820 AT3G11260.1 59.701 67 27 0 39 105 24 90 3.27e-26 98.6
Msa0610820 AT1G46480.2 63.768 69 24 1 33 101 85 152 4.53e-26 98.2
Msa0610820 AT2G17950.1 60.759 79 30 1 35 112 34 112 5.23e-26 100
Msa0610820 AT2G01500.1 74.545 55 14 0 39 93 61 115 1.44e-25 99.0
Msa0610820 AT1G46480.1 63.768 69 24 1 33 101 85 152 2.66e-25 97.8
Msa0610820 AT5G05770.1 59.375 64 26 0 39 102 29 92 8.14e-23 88.2
Msa0610820 AT2G33880.1 44.286 70 39 0 34 103 50 119 2.14e-17 78.2
Msa0610820 AT2G33880.2 44.286 70 39 0 34 103 50 119 2.36e-17 78.2
Msa0610820 AT5G45980.1 38.824 85 52 0 16 100 32 116 9.71e-17 76.3
Msa0610820 AT4G35550.1 49.180 61 31 0 39 99 99 159 9.81e-16 72.8
Msa0610820 AT1G20700.2 45.000 60 33 0 39 98 33 92 2.70e-14 66.6
Msa0610820 AT1G20700.3 45.000 60 33 0 39 98 95 154 8.41e-14 66.6
Msa0610820 AT1G20700.1 45.000 60 33 0 39 98 95 154 9.75e-14 66.6
Msa0610820 AT3G03660.1 49.057 53 27 0 38 90 32 84 6.24e-13 65.1
Msa0610820 AT3G03660.2 49.057 53 27 0 38 90 32 84 8.82e-13 65.1
Msa0610820 AT3G03660.4 49.057 53 27 0 38 90 32 84 1.05e-12 64.7
Msa0610820 AT3G03660.3 49.057 53 27 0 38 90 32 84 1.41e-12 64.7
Msa0610820 AT5G17810.1 43.396 53 30 0 38 90 20 72 3.43e-11 60.1
Msa0610820 AT5G17810.2 43.396 53 30 0 38 90 20 72 3.43e-11 60.1
Msa0610820 AT5G17810.3 43.396 53 30 0 38 90 20 72 3.44e-11 60.1

Find 29 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTCTCGCCTTGTGATTC+TGG 0.194023 4_2:-58712646 None:intergenic
GCTTCGCAGCTTCGACAATT+TGG 0.206061 4_2:+58712590 Msa0610820:CDS
ATTTCTCTTGTCTTTGTTGA+TGG 0.263889 4_2:+58713343 Msa0610820:CDS
AGAGAAAGAGAAGTACGTTA+TGG 0.359866 4_2:+58712733 Msa0610820:CDS
CGACAATTTGGAAAGATTGA+AGG 0.396848 4_2:+58712602 Msa0610820:CDS
GAGAAAGAGAAGTACGTTAT+GGG 0.401887 4_2:+58712734 Msa0610820:CDS
GAAGCTGCGAAGCTATTTGT+TGG 0.428703 4_2:-58712580 None:intergenic
CTTGCCTATACAATTCTTCA+AGG 0.428836 4_2:-58712529 None:intergenic
TAGTCCTTGAAGAATTGTAT+AGG 0.431151 4_2:+58712525 Msa0610820:CDS
GTTGGACTCCACCTTGAACT+TGG 0.444756 4_2:-58712488 None:intergenic
CTTGAGATGGAGGAAACAAT+TGG 0.464032 4_2:+58712686 Msa0610820:CDS
TGCATCCTCAACCAAGTTCA+AGG 0.472729 4_2:+58712477 Msa0610820:CDS
CTCCACCTTGAACTTGGTTG+AGG 0.502185 4_2:-58712482 None:intergenic
TGTTGGATTTGTTCAGCTGA+TGG 0.503667 4_2:-58712563 None:intergenic
TTGTTCACTGCAGTTTGAAG+TGG 0.506780 4_2:-58713307 None:intergenic
TCAAGGACTAGTAATTGAAC+TGG 0.509163 4_2:-58712512 None:intergenic
GAAGCGTCGTCGCCTTGAGA+TGG 0.530642 4_2:+58712673 Msa0610820:CDS
ACTAGTAATTGAACTGGTGT+TGG 0.537149 4_2:-58712506 None:intergenic
CTTGAAGAATTGTATAGGCA+AGG 0.540070 4_2:+58712530 Msa0610820:CDS
TCAGAAAAGCAAGAAGCTGC+AGG 0.540109 4_2:+58712761 Msa0610820:CDS
CAATTGTTTCCTCCATCTCA+AGG 0.548253 4_2:-58712685 None:intergenic
GCGTCGTCGCCTTGAGATGG+AGG 0.576407 4_2:+58712676 Msa0610820:CDS
TTGAAGAATTGTATAGGCAA+GGG 0.590766 4_2:+58712531 Msa0610820:CDS
ACAGCTCATGCAGCTGAGCA+AGG 0.595931 4_2:+58712440 Msa0610820:CDS
AAACTGCAGTGAACAAGCAG+AGG 0.632631 4_2:+58713314 Msa0610820:CDS
AGTTAAAGAGACAAAGAAGT+GGG 0.667079 4_2:+58713281 Msa0610820:CDS
CTATTGGTTCCAGAATCACA+AGG 0.685006 4_2:+58712637 Msa0610820:CDS
GAGTTAAAGAGACAAAGAAG+TGG 0.689037 4_2:+58713280 Msa0610820:CDS
ATCCTCAACCAAGTTCAAGG+TGG 0.762805 4_2:+58712480 Msa0610820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTTCTTTCAAATA+TGG - chr4_2:58713107-58713126 None:intergenic 10.0%
!!! AAATTTTTTTCTTTCAAATA+TGG - chr4_2:58713107-58713126 None:intergenic 10.0%
!!! TTGTTGTTTATTCAATTTAA+TGG + chr4_2:58713128-58713147 Msa0610820:intron 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG + chr4_2:58713159-58713178 Msa0610820:intron 15.0%
!!! TTGTTGTTTATTCAATTTAA+TGG + chr4_2:58713128-58713147 Msa0610820:intron 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG + chr4_2:58713159-58713178 Msa0610820:intron 15.0%
!! TCATGAGTGAAATATAATAT+AGG - chr4_2:58712845-58712864 None:intergenic 20.0%
!! AGAAAAAAAAATGACCAAAT+GGG - chr4_2:58712967-58712986 None:intergenic 20.0%
!! AAGAAAAAAAAATGACCAAA+TGG - chr4_2:58712968-58712987 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG - chr4_2:58713000-58713019 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG - chr4_2:58713083-58713102 None:intergenic 20.0%
!! TCATGAGTGAAATATAATAT+AGG - chr4_2:58712845-58712864 None:intergenic 20.0%
!! AGAAAAAAAAATGACCAAAT+GGG - chr4_2:58712967-58712986 None:intergenic 20.0%
!! AAGAAAAAAAAATGACCAAA+TGG - chr4_2:58712968-58712987 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG - chr4_2:58713000-58713019 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG - chr4_2:58713083-58713102 None:intergenic 20.0%
! AAGGAAAAAATGTGTTCTAT+TGG + chr4_2:58712621-58712640 Msa0610820:CDS 25.0%
! TACAGCTAGATATTATCTTT+AGG + chr4_2:58712786-58712805 Msa0610820:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG + chr4_2:58712787-58712806 Msa0610820:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG + chr4_2:58712797-58712816 Msa0610820:intron 25.0%
!! AAAGATACATAGCTTTTGTA+AGG + chr4_2:58712901-58712920 Msa0610820:intron 25.0%
! AAATATCAATTTAGCCCATT+TGG + chr4_2:58712950-58712969 Msa0610820:intron 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG - chr4_2:58713001-58713020 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG + chr4_2:58712998-58713017 Msa0610820:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG + chr4_2:58713052-58713071 Msa0610820:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG + chr4_2:58713228-58713247 Msa0610820:intron 25.0%
! AAGGAAAAAATGTGTTCTAT+TGG + chr4_2:58712621-58712640 Msa0610820:CDS 25.0%
! TACAGCTAGATATTATCTTT+AGG + chr4_2:58712786-58712805 Msa0610820:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG + chr4_2:58712787-58712806 Msa0610820:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG + chr4_2:58712797-58712816 Msa0610820:intron 25.0%
!! AAAGATACATAGCTTTTGTA+AGG + chr4_2:58712901-58712920 Msa0610820:intron 25.0%
! AAATATCAATTTAGCCCATT+TGG + chr4_2:58712950-58712969 Msa0610820:intron 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG - chr4_2:58713001-58713020 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG + chr4_2:58712998-58713017 Msa0610820:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG + chr4_2:58713052-58713071 Msa0610820:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG + chr4_2:58713228-58713247 Msa0610820:intron 25.0%
TAGTCCTTGAAGAATTGTAT+AGG + chr4_2:58712525-58712544 Msa0610820:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG + chr4_2:58712531-58712550 Msa0610820:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG + chr4_2:58712809-58712828 Msa0610820:intron 30.0%
ACACACACTTCAAATTGTAA+GGG - chr4_2:58713198-58713217 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG + chr4_2:58713281-58713300 Msa0610820:CDS 30.0%
TAGTCCTTGAAGAATTGTAT+AGG + chr4_2:58712525-58712544 Msa0610820:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG + chr4_2:58712531-58712550 Msa0610820:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG + chr4_2:58712809-58712828 Msa0610820:intron 30.0%
ACACACACTTCAAATTGTAA+GGG - chr4_2:58713198-58713217 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG + chr4_2:58713281-58713300 Msa0610820:CDS 30.0%
ACTAGTAATTGAACTGGTGT+TGG - chr4_2:58712509-58712528 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG - chr4_2:58712515-58712534 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG - chr4_2:58712532-58712551 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG + chr4_2:58712530-58712549 Msa0610820:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG + chr4_2:58712602-58712621 Msa0610820:CDS 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG + chr4_2:58712733-58712752 Msa0610820:CDS 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG + chr4_2:58712734-58712753 Msa0610820:CDS 35.0%
! CATAGCTTTTGTAAGGATCT+CGG + chr4_2:58712908-58712927 Msa0610820:intron 35.0%
CACACACACTTCAAATTGTA+AGG - chr4_2:58713199-58713218 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG + chr4_2:58713235-58713254 Msa0610820:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG + chr4_2:58713252-58713271 Msa0610820:intron 35.0%
GAGTTAAAGAGACAAAGAAG+TGG + chr4_2:58713280-58713299 Msa0610820:CDS 35.0%
ACTAGTAATTGAACTGGTGT+TGG - chr4_2:58712509-58712528 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG - chr4_2:58712515-58712534 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG - chr4_2:58712532-58712551 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG + chr4_2:58712530-58712549 Msa0610820:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG + chr4_2:58712602-58712621 Msa0610820:CDS 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG + chr4_2:58712733-58712752 Msa0610820:CDS 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG + chr4_2:58712734-58712753 Msa0610820:CDS 35.0%
! CATAGCTTTTGTAAGGATCT+CGG + chr4_2:58712908-58712927 Msa0610820:intron 35.0%
CACACACACTTCAAATTGTA+AGG - chr4_2:58713199-58713218 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG + chr4_2:58713235-58713254 Msa0610820:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG + chr4_2:58713252-58713271 Msa0610820:intron 35.0%
GAGTTAAAGAGACAAAGAAG+TGG + chr4_2:58713280-58713299 Msa0610820:CDS 35.0%
! TGTTGGATTTGTTCAGCTGA+TGG - chr4_2:58712566-58712585 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG + chr4_2:58712637-58712656 Msa0610820:CDS 40.0%
CAATTGTTTCCTCCATCTCA+AGG - chr4_2:58712688-58712707 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG + chr4_2:58712686-58712705 Msa0610820:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG + chr4_2:58713255-58713274 Msa0610820:intron 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG + chr4_2:58713258-58713277 Msa0610820:intron 40.0%
TTGTTCACTGCAGTTTGAAG+TGG - chr4_2:58713310-58713329 None:intergenic 40.0%
! TGTTGGATTTGTTCAGCTGA+TGG - chr4_2:58712566-58712585 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG + chr4_2:58712637-58712656 Msa0610820:CDS 40.0%
CAATTGTTTCCTCCATCTCA+AGG - chr4_2:58712688-58712707 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG + chr4_2:58712686-58712705 Msa0610820:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG + chr4_2:58713255-58713274 Msa0610820:intron 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG + chr4_2:58713258-58713277 Msa0610820:intron 40.0%
TTGTTCACTGCAGTTTGAAG+TGG - chr4_2:58713310-58713329 None:intergenic 40.0%
TGCATCCTCAACCAAGTTCA+AGG + chr4_2:58712477-58712496 Msa0610820:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG + chr4_2:58712480-58712499 Msa0610820:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG - chr4_2:58712583-58712602 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG + chr4_2:58712761-58712780 Msa0610820:CDS 45.0%
AAACTGCAGTGAACAAGCAG+AGG + chr4_2:58713314-58713333 Msa0610820:CDS 45.0%
TGCATCCTCAACCAAGTTCA+AGG + chr4_2:58712477-58712496 Msa0610820:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG + chr4_2:58712480-58712499 Msa0610820:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG - chr4_2:58712583-58712602 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG + chr4_2:58712761-58712780 Msa0610820:CDS 45.0%
AAACTGCAGTGAACAAGCAG+AGG + chr4_2:58713314-58713333 Msa0610820:CDS 45.0%
CTCCACCTTGAACTTGGTTG+AGG - chr4_2:58712485-58712504 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG - chr4_2:58712491-58712510 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG + chr4_2:58712590-58712609 Msa0610820:CDS 50.0%
CTCCACCTTGAACTTGGTTG+AGG - chr4_2:58712485-58712504 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG - chr4_2:58712491-58712510 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG + chr4_2:58712590-58712609 Msa0610820:CDS 50.0%
ACAGCTCATGCAGCTGAGCA+AGG + chr4_2:58712440-58712459 Msa0610820:CDS 55.0%
GCTCTCTCGCCTTGTGATTC+TGG - chr4_2:58712649-58712668 None:intergenic 55.0%
ACAGCTCATGCAGCTGAGCA+AGG + chr4_2:58712440-58712459 Msa0610820:CDS 55.0%
GCTCTCTCGCCTTGTGATTC+TGG - chr4_2:58712649-58712668 None:intergenic 55.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG + chr4_2:58712673-58712692 Msa0610820:CDS 60.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG + chr4_2:58712673-58712692 Msa0610820:CDS 60.0%
GCGTCGTCGCCTTGAGATGG+AGG + chr4_2:58712676-58712695 Msa0610820:CDS 65.0%
GCGTCGTCGCCTTGAGATGG+AGG + chr4_2:58712676-58712695 Msa0610820:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 58712383 58713362 58712383 ID=Msa0610820;Name=Msa0610820
chr4_2 mRNA 58712383 58713362 58712383 ID=Msa0610820-mRNA-1;Parent=Msa0610820;Name=Msa0610820-mRNA-1;_AED=0.16;_eAED=0.19;_QI=0|0|0|1|1|1|2|0|164
chr4_2 exon 58712383 58712782 58712383 ID=Msa0610820-mRNA-1:exon:15481;Parent=Msa0610820-mRNA-1
chr4_2 exon 58713268 58713362 58713268 ID=Msa0610820-mRNA-1:exon:15482;Parent=Msa0610820-mRNA-1
chr4_2 CDS 58712383 58712782 58712383 ID=Msa0610820-mRNA-1:cds;Parent=Msa0610820-mRNA-1
chr4_2 CDS 58713268 58713362 58713268 ID=Msa0610820-mRNA-1:cds;Parent=Msa0610820-mRNA-1
Gene Sequence

>Msa0610820

ATGTGTTTTTTTTTTTTTTTTGAACAATTTTCTTCATGTGTTTTGTATATATATTATACAGCTCATGCAGCTGAGCAAGGTACAAGCAATACTATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAGGTCTGTAATTTCTCTTGTCTTTGTTGA

Protein sequence

>Msa0610820

MCFFFFFEQFSSCVLYIYYTAHAAEQGTSNTMHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRRRLEMEETIGSSSTEDKKEKEKYVMGNSEKQEAAETGGGVKETKKWATTSNCSEQAEVCNFSCLC*