AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0611690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0611690 MtrunA17_Chr4g0045361 94.286 420 20 2 1 419 1 417 0.0 717
Msa0611690 MtrunA17_Chr4g0062921 39.788 377 158 14 7 367 5 328 3.18e-65 214
Msa0611690 MtrunA17_Chr1g0198601 35.097 359 143 12 44 371 10 309 5.35e-46 162
Msa0611690 MtrunA17_Chr5g0422621 35.942 345 142 12 41 371 16 295 1.79e-44 158
Msa0611690 MtrunA17_Chr5g0402631 58.993 139 51 3 274 409 211 346 1.47e-43 155
Msa0611690 MtrunA17_Chr1g0175081 47.059 68 36 0 274 341 19 86 2.74e-15 71.2
Msa0611690 MtrunA17_Chr7g0255711 50.746 67 32 1 270 336 84 149 3.15e-15 75.9
Msa0611690 MtrunA17_Chr7g0243081 40.698 86 51 0 245 330 39 124 3.59e-14 73.6
Msa0611690 MtrunA17_Chr2g0288601 50.769 65 32 0 263 327 57 121 4.69e-14 68.9
Msa0611690 MtrunA17_Chr7g0261911 51.471 68 33 0 277 344 262 329 6.65e-14 73.2
Msa0611690 MtrunA17_Chr5g0417521 55.172 58 26 0 274 331 12 69 7.88e-14 72.4
Msa0611690 MtrunA17_Chr1g0190301 61.111 54 21 0 277 330 175 228 1.20e-13 72.0
Msa0611690 MtrunA17_Chr8g0366281 48.980 98 43 4 251 341 142 239 1.35e-13 71.6
Msa0611690 MtrunA17_Chr7g0254981 49.315 73 36 1 269 341 42 113 1.59e-13 70.5
Msa0611690 MtrunA17_Chr2g0304841 48.052 77 35 3 276 351 212 284 1.71e-13 72.4
Msa0611690 MtrunA17_Chr7g0274471 62.963 54 20 0 277 330 218 271 2.07e-13 71.6
Msa0611690 MtrunA17_Chr1g0149841 50.000 68 33 1 272 339 186 252 2.22e-13 71.6
Msa0611690 MtrunA17_Chr5g0400241 47.826 69 35 1 268 336 302 369 2.29e-13 71.6
Msa0611690 MtrunA17_Chr4g0067981 53.731 67 30 1 274 340 212 277 2.42e-13 72.0
Msa0611690 MtrunA17_Chr4g0031961 47.059 68 35 1 273 340 127 193 2.90e-13 70.1
Msa0611690 MtrunA17_Chr1g0162931 63.265 49 18 0 282 330 23 71 3.94e-13 69.3
Msa0611690 MtrunA17_Chr4g0040481 43.820 89 45 2 273 357 154 241 4.23e-13 70.5
Msa0611690 MtrunA17_Chr2g0294911 50.746 67 32 1 274 340 208 273 8.01e-13 70.1
Msa0611690 MtrunA17_Chr1g0210911 61.224 49 19 0 282 330 27 75 8.11e-13 69.3
Msa0611690 MtrunA17_Chr3g0137101 42.529 87 38 2 276 351 197 282 8.42e-13 70.1
Msa0611690 MtrunA17_Chr2g0291021 60.000 55 22 0 277 331 49 103 9.37e-13 68.9
Msa0611690 MtrunA17_Chr4g0054981 31.250 128 76 2 209 336 253 368 1.16e-12 69.7
Msa0611690 MtrunA17_Chr5g0410741 46.465 99 48 2 248 341 187 285 1.26e-12 68.9
Msa0611690 MtrunA17_Chr2g0321461 53.448 58 27 0 274 331 42 99 2.20e-12 68.2
Msa0611690 MtrunA17_Chr6g0479431 35.616 146 83 4 264 402 184 325 2.47e-12 67.8
Msa0611690 MtrunA17_Chr1g0149831 48.438 64 29 1 276 339 127 186 3.23e-12 65.9
Msa0611690 MtrunA17_Chr6g0461641 51.724 58 28 0 274 331 39 96 3.32e-12 67.8
Msa0611690 MtrunA17_Chr1g0161431 47.761 67 34 1 274 340 197 262 3.76e-12 68.2
Msa0611690 MtrunA17_Chr1g0175091 53.448 58 27 0 274 331 255 312 5.56e-12 67.0
Msa0611690 MtrunA17_Chr1g0154261 35.338 133 77 3 272 403 121 245 1.14e-11 65.5
Msa0611690 MtrunA17_Chr4g0074411 45.205 73 39 1 274 346 198 269 1.15e-11 66.6
Msa0611690 MtrunA17_Chr5g0409731 54.098 61 26 1 277 335 25 85 1.28e-11 65.1
Msa0611690 MtrunA17_Chr8g0356501 55.172 58 26 0 274 331 34 91 1.38e-11 65.5
Msa0611690 MtrunA17_Chr4g0042451 51.724 58 28 0 274 331 14 71 1.70e-11 65.1
Msa0611690 MtrunA17_Chr4g0009901 49.180 61 30 1 275 335 166 225 2.26e-11 64.3
Msa0611690 MtrunA17_Chr2g0324891 49.180 61 30 1 276 336 160 219 2.47e-11 65.1
Msa0611690 MtrunA17_Chr3g0095611 50.000 56 28 0 275 330 17 72 2.49e-11 63.2
Msa0611690 MtrunA17_Chr1g0197171 56.364 55 24 0 277 331 44 98 2.55e-11 64.3
Msa0611690 MtrunA17_Chr2g0310011 55.172 58 26 0 274 331 32 89 2.78e-11 64.7
Msa0611690 MtrunA17_Chr3g0109981 49.315 73 36 1 269 340 266 338 3.03e-11 65.1
Msa0611690 MtrunA17_Chr2g0320231 45.455 66 36 0 265 330 85 150 4.84e-11 63.9
Msa0611690 MtrunA17_Chr7g0258551 50.877 57 28 0 274 330 209 265 5.70e-11 63.9
Msa0611690 MtrunA17_Chr2g0290991 56.364 55 24 0 277 331 39 93 5.93e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0611690 AT2G03500.1 40.533 375 158 10 7 368 5 327 5.49e-64 212
Msa0611690 AT4G37180.1 35.027 374 159 10 9 377 12 306 5.19e-49 170
Msa0611690 AT4G37180.2 34.840 376 164 12 9 377 12 313 5.33e-47 165
Msa0611690 AT4G37180.3 35.110 319 137 8 9 327 12 260 1.71e-42 151
Msa0611690 AT1G49560.1 53.797 158 56 6 261 405 172 325 2.85e-37 138
Msa0611690 AT1G68670.1 69.892 93 20 1 274 358 212 304 7.63e-36 135
Msa0611690 AT1G68670.1 35.000 120 62 4 36 139 8 127 1.20e-11 66.2
Msa0611690 AT1G13300.1 70.787 89 21 2 276 359 180 268 5.24e-34 130
Msa0611690 AT1G25550.1 54.622 119 47 2 274 385 206 324 2.53e-33 128
Msa0611690 AT3G25790.1 57.292 96 36 1 281 371 200 295 1.81e-30 120
Msa0611690 AT1G49560.2 66.667 81 21 2 251 327 164 242 6.44e-25 103
Msa0611690 AT1G25550.2 83.333 54 9 0 274 327 206 259 1.17e-24 102
Msa0611690 AT3G25790.2 78.723 47 10 0 281 327 200 246 1.64e-19 88.2
Msa0611690 AT3G10760.1 50.704 71 34 1 266 336 94 163 2.77e-15 77.0
Msa0611690 AT5G05090.1 50.746 67 32 1 270 336 74 139 2.88e-15 75.9
Msa0611690 AT2G40970.1 50.746 67 32 1 270 336 98 163 4.65e-15 75.1
Msa0611690 AT2G38300.1 46.053 76 41 0 255 330 32 107 1.56e-14 74.7
Msa0611690 AT5G59570.2 50.000 64 31 1 273 336 137 199 1.77e-14 73.9
Msa0611690 AT5G59570.1 50.000 64 31 1 273 336 137 199 1.77e-14 73.9
Msa0611690 AT2G06020.1 35.652 115 72 1 232 346 41 153 1.82e-13 71.2
Msa0611690 AT3G04450.3 50.685 73 35 1 258 330 220 291 1.89e-13 71.2
Msa0611690 AT5G58080.1 50.794 63 30 1 274 336 191 252 3.57e-13 71.6
Msa0611690 AT3G16857.3 43.137 102 44 2 276 377 166 253 3.57e-13 71.6
Msa0611690 AT3G16857.2 43.137 102 44 2 276 377 236 323 3.75e-13 71.6
Msa0611690 AT3G04450.1 43.333 90 50 1 258 347 220 308 3.78e-13 71.2
Msa0611690 AT3G46640.2 48.438 64 32 1 273 336 140 202 4.00e-13 70.5
Msa0611690 AT3G46640.1 48.438 64 32 1 273 336 140 202 4.00e-13 70.5
Msa0611690 AT3G16857.1 43.137 102 44 2 276 377 236 323 4.03e-13 71.6
Msa0611690 AT3G13040.3 58.929 56 23 0 276 331 240 295 4.54e-13 71.2
Msa0611690 AT3G13040.1 58.929 56 23 0 276 331 240 295 4.54e-13 71.2
Msa0611690 AT3G13040.2 58.929 56 23 0 276 331 240 295 4.54e-13 71.2
Msa0611690 AT3G04450.4 43.333 90 50 1 258 347 180 268 4.96e-13 70.9
Msa0611690 AT3G04450.2 43.333 90 50 1 258 347 180 268 4.96e-13 70.9
Msa0611690 AT2G01760.3 54.839 62 27 1 275 336 133 193 5.16e-13 70.1
Msa0611690 AT3G46640.3 48.438 64 32 1 273 336 140 202 5.56e-13 70.1
Msa0611690 AT4G28610.1 52.239 67 31 1 277 342 225 291 5.73e-13 70.5
Msa0611690 AT2G01760.2 54.839 62 27 1 275 336 226 286 6.35e-13 70.5
Msa0611690 AT2G01760.1 54.839 62 27 1 275 336 198 258 6.56e-13 70.1
Msa0611690 AT2G01060.1 60.000 55 22 0 277 331 15 69 8.79e-13 68.9
Msa0611690 AT5G29000.4 56.140 57 25 0 274 330 228 284 1.51e-12 68.6
Msa0611690 AT5G18240.2 55.172 58 26 0 274 331 42 99 1.77e-12 68.9
Msa0611690 AT5G18240.3 55.172 58 26 0 274 331 42 99 1.77e-12 68.9
Msa0611690 AT2G20400.1 53.125 64 29 1 277 340 231 293 1.95e-12 68.9
Msa0611690 AT2G20400.2 50.000 70 34 1 277 345 231 300 1.97e-12 68.9
Msa0611690 AT5G18240.5 55.172 58 26 0 274 331 42 99 2.02e-12 68.9
Msa0611690 AT5G18240.1 55.172 58 26 0 274 331 42 99 2.15e-12 68.9
Msa0611690 AT5G18240.4 55.172 58 26 0 274 331 42 99 2.15e-12 68.9
Msa0611690 AT3G04030.1 53.448 58 27 0 274 331 42 99 2.37e-12 68.6
Msa0611690 AT3G04030.3 53.448 58 27 0 274 331 42 99 2.68e-12 68.6
Msa0611690 AT5G29000.2 59.259 54 22 0 277 330 231 284 4.06e-12 67.8
Msa0611690 AT5G29000.1 59.259 54 22 0 277 330 188 241 4.25e-12 67.8
Msa0611690 AT5G29000.3 59.259 54 22 0 277 330 188 241 4.25e-12 67.8
Msa0611690 AT5G06800.3 44.156 77 40 2 255 331 173 246 6.55e-12 66.2
Msa0611690 AT2G02060.2 50.000 62 31 0 269 330 21 82 6.64e-12 65.9
Msa0611690 AT2G02060.1 50.000 62 31 0 269 330 21 82 6.64e-12 65.9
Msa0611690 AT1G14600.1 51.613 62 30 0 269 330 15 76 7.08e-12 65.9
Msa0611690 AT5G45580.1 48.529 68 35 0 277 344 7 74 7.29e-12 65.9
Msa0611690 AT2G25180.1 46.154 65 34 1 276 340 194 257 7.82e-12 67.4
Msa0611690 AT5G45580.2 47.826 69 36 0 277 345 23 91 7.89e-12 65.9
Msa0611690 AT1G32240.2 45.455 88 47 1 265 351 200 287 9.23e-12 66.6
Msa0611690 AT3G12730.1 55.172 58 26 0 274 331 20 77 1.11e-11 64.7
Msa0611690 AT1G32240.1 45.455 88 47 1 265 351 200 287 1.15e-11 66.6
Msa0611690 AT5G16560.1 50.000 78 37 2 265 340 206 283 2.65e-11 65.5
Msa0611690 AT1G67710.3 51.724 58 28 0 279 336 121 178 2.81e-11 65.5
Msa0611690 AT1G67710.2 51.724 58 28 0 279 336 130 187 2.92e-11 65.5
Msa0611690 AT1G67710.1 51.724 58 28 0 279 336 195 252 3.64e-11 65.5
Msa0611690 AT5G06800.1 44.156 77 40 2 255 331 173 246 4.50e-11 64.7
Msa0611690 AT5G06800.2 44.156 77 40 2 255 331 173 246 4.51e-11 64.7
Msa0611690 AT1G79430.2 55.172 58 26 0 274 331 31 88 4.55e-11 64.3
Msa0611690 AT3G24120.1 55.172 58 26 0 274 331 38 95 5.18e-11 63.5
Msa0611690 AT4G13640.1 56.364 55 24 0 277 331 37 91 5.64e-11 63.5
Msa0611690 AT4G13640.2 56.364 55 24 0 277 331 37 91 5.73e-11 63.5
Msa0611690 AT3G24120.2 56.364 55 24 0 277 331 41 95 5.87e-11 63.5
Msa0611690 AT2G42660.1 55.102 49 22 0 282 330 55 103 8.62e-11 62.4
Msa0611690 AT2G40260.1 46.154 65 35 0 266 330 71 135 9.74e-11 63.5

Find 130 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAGTTCTTGGAGGTTTA+TGG 0.186325 4_2:+60021574 Msa0611690:CDS
GTGATGAGATTTATATAAAA+TGG 0.205392 4_2:-60019227 None:intergenic
CTCTTTCCTTTCTGCATTGT+AGG 0.213979 4_2:+60022314 Msa0611690:three_prime_UTR
ATTCTTGTTGAGGAGTTAAA+AGG 0.219449 4_2:-60019270 None:intergenic
GGTTCTTCCTTCTCTTCTTT+AGG 0.234160 4_2:-60020285 None:intergenic
AAAGAGAGCCTATTCCTTAC+AGG 0.270823 4_2:-60020306 None:intergenic
GAGGAAATTAGCGATGGTTT+TGG 0.303370 4_2:-60019418 None:intergenic
TTCAGCCGCAGCAGACTTCT+AGG 0.305420 4_2:+60020454 Msa0611690:CDS
TTTAGTCCAATGTTATGTTT+TGG 0.308011 4_2:+60022065 Msa0611690:three_prime_UTR
GATCAGTCTGTTGTAGTTCT+TGG 0.310407 4_2:+60021564 Msa0611690:CDS
ACAGAAGAATACGGTGAAAA+AGG 0.316862 4_2:-60020234 None:intergenic
ACAAATCTCCGGTGCAATTC+CGG 0.337882 4_2:-60020498 None:intergenic
GGAATAAACTCTTCCAAAAC+TGG 0.355348 4_2:-60019802 None:intergenic
TTGTAGGGTTGTAGCCTTGT+AGG 0.361502 4_2:+60022330 Msa0611690:three_prime_UTR
TAGATGTGAATGTGAAATTA+AGG 0.363270 4_2:-60019111 None:intergenic
TCCACAGAGTCCACTTCATT+TGG 0.366064 4_2:+60021653 Msa0611690:CDS
ATGGAGTTTGGAGAGTTTAT+CGG 0.366175 4_2:-60019469 None:intergenic
ATGAGTTCTCTAATTTGCTT+TGG 0.366259 4_2:-60021141 None:intergenic
GTAGGATTAAGAGAAGATAA+TGG 0.366733 4_2:-60022419 None:intergenic
AGAGTTTATCGGAGGTTGTT+TGG 0.367071 4_2:-60019458 None:intergenic
AGAGAGGAAATCATGGAGTT+TGG 0.369125 4_2:-60019481 None:intergenic
GATCGAGAAACAGTTCTAAC+AGG 0.377734 4_2:+60020376 Msa0611690:CDS
GCAGCAAAGATAAGAAGAAT+TGG 0.382485 4_2:+60019896 Msa0611690:CDS
AGAGAACTCATGCAAGTTGA+TGG 0.388920 4_2:+60021153 Msa0611690:CDS
GGATGAGTTCTGTTCAGTTA+TGG 0.403657 4_2:+60019917 Msa0611690:CDS
CGCATACTCGGCTGCACGGT+TGG 0.404437 4_2:-60020420 None:intergenic
AAGAGTCTGAGGGAAGGATT+TGG 0.407195 4_2:+60020345 Msa0611690:CDS
TGATGAGATTTATATAAAAT+GGG 0.411321 4_2:-60019226 None:intergenic
ATTTATCACTTGTGAAATTG+AGG 0.416780 4_2:+60022445 Msa0611690:three_prime_UTR
AGGAAGAACCTGTAAGGAAT+AGG 0.418786 4_2:+60020298 Msa0611690:CDS
TTCTTGTTGAGGAGTTAAAA+GGG 0.425040 4_2:-60019269 None:intergenic
CAGTCTGTTGTAGTTCTTGG+AGG 0.429416 4_2:+60021567 Msa0611690:CDS
AGACGCCGTACTGCTACCTT+TGG 0.431495 4_2:-60021625 None:intergenic
TCTTTCCTTTCTGCATTGTA+GGG 0.437001 4_2:+60022315 Msa0611690:three_prime_UTR
AGAGTATCGGTTCTCATAGT+AGG 0.438676 4_2:+60022370 Msa0611690:three_prime_UTR
TACATTCCACGATAAGAATC+AGG 0.439928 4_2:+60021976 Msa0611690:three_prime_UTR
ATCGGAGGTTGTTTGGATTG+TGG 0.443195 4_2:-60019451 None:intergenic
TCTTCACTTCATCATTAGTC+AGG 0.447623 4_2:-60021176 None:intergenic
AGTACGAACCGTCGTTTAAG+AGG 0.448869 4_2:-60019566 None:intergenic
GAAGAAGAAGATTCTTGTTG+AGG 0.451264 4_2:-60019280 None:intergenic
CATCTCTGAAATTTGTGAAG+TGG 0.455715 4_2:-60022127 None:intergenic
CCTTGTAGTAGTAATAGAAA+TGG 0.456389 4_2:+60020195 Msa0611690:CDS
CAGTTGGTGATGTCCCTCCT+CGG 0.457090 4_2:-60021686 None:intergenic
ACATGGAAAAGTTGGTGTAT+AGG 0.458002 4_2:+60021776 Msa0611690:CDS
GAATACATAGAAGAGGCTAT+TGG 0.458107 4_2:+60021878 Msa0611690:three_prime_UTR
GTTAATGCGTTGCAGAAGCT+TGG 0.458197 4_2:+60020519 Msa0611690:CDS
TCCCTCAGACTCTTCACCTC+AGG 0.461715 4_2:-60020336 None:intergenic
CCATTTCTATTACTACTACA+AGG 0.463294 4_2:-60020195 None:intergenic
AAAGGTAGCAGTACGGCGTC+TGG 0.473278 4_2:+60021627 Msa0611690:CDS
TGATCAATCTCTTTCTTCAA+AGG 0.473313 4_2:-60019823 None:intergenic
TGTAGGGTTGTAGCCTTGTA+GGG 0.473411 4_2:+60022331 Msa0611690:three_prime_UTR
AGTCCACTTCATTTGGCTAC+AGG 0.481755 4_2:+60021660 Msa0611690:CDS
AGATTTATATAAAATGGGAA+TGG 0.481854 4_2:-60019221 None:intergenic
AAATCTAAACTGAGTTCTGC+AGG 0.491660 4_2:-60019378 None:intergenic
TGAGGGAAGGATTTGGTTCG+AGG 0.493121 4_2:+60020352 Msa0611690:CDS
TACTACTACAAGGTTGAAAA+CGG 0.497197 4_2:-60020185 None:intergenic
ATTCACAGACATGGAAAAGT+TGG 0.497674 4_2:+60021768 Msa0611690:CDS
GATTTATATAAAATGGGAAT+GGG 0.500404 4_2:-60019220 None:intergenic
AAGAGAAGGAAGAACCTGTA+AGG 0.506152 4_2:+60020292 Msa0611690:CDS
AGCGGCGCAGCACGCATACT+CGG 0.508461 4_2:-60020432 None:intergenic
AGTTGGTGATGTCCCTCCTC+GGG 0.513085 4_2:-60021685 None:intergenic
ATCAGCACCTAAAGAAGAGA+AGG 0.517481 4_2:+60020278 Msa0611690:CDS
TGATTCTTATCGTGGAATGT+AGG 0.518021 4_2:-60021974 None:intergenic
CAGAAGCTTGGAGGTTCTCA+AGG 0.522479 4_2:+60020531 Msa0611690:CDS
TTTGCAGATAAAAGTATACA+TGG 0.523808 4_2:+60021821 Msa0611690:three_prime_UTR
CTAGGAAACAGAGGAGATGC+TGG 0.524226 4_2:+60020472 Msa0611690:CDS
CGAGTCGAGAGAGGAAATCA+TGG 0.525149 4_2:-60019488 None:intergenic
CTCCTGAGGTGAAGAGTCTG+AGG 0.527871 4_2:+60020334 Msa0611690:CDS
GAGTCGTTGTATTGTTCTTG+AGG 0.536314 4_2:-60021600 None:intergenic
GAAGTCTGCTGCGGCTGAAG+CGG 0.537379 4_2:-60020450 None:intergenic
TGATCAGTACCACTTGCAGC+TGG 0.538762 4_2:-60021546 None:intergenic
ATACTCGGCTGCACGGTTGG+AGG 0.545035 4_2:-60020417 None:intergenic
AATGCGTTGCAGAAGCTTGG+AGG 0.554542 4_2:+60020522 Msa0611690:CDS
AGAGAAAGCAACGACGAATG+AGG 0.554931 4_2:-60019034 None:intergenic
CATCCACCAAAACATAACAT+TGG 0.559517 4_2:-60022071 None:intergenic
ACAGAGGAGATGCTGGTCAC+CGG 0.560535 4_2:+60020479 Msa0611690:CDS
GAACTCTCCGCGTATGAAGT+CGG 0.562568 4_2:-60021524 None:intergenic
AAATGACCTGATTCTTATCG+TGG 0.563659 4_2:-60021982 None:intergenic
GTTCGATTTAGATAGAGTAT+CGG 0.565543 4_2:+60022357 Msa0611690:three_prime_UTR
TCTTGTTGAGGAGTTAAAAG+GGG 0.565631 4_2:-60019268 None:intergenic
GCTGGTCACCGGAATTGCAC+CGG 0.565693 4_2:+60020490 Msa0611690:CDS
GCAACGCATTAACAAATCTC+CGG 0.573053 4_2:-60020509 None:intergenic
TAAATCGAACAGTCCCTACA+AGG 0.577392 4_2:-60022344 None:intergenic
TCACAAGTGATAAATAGAGT+AGG 0.583966 4_2:-60022437 None:intergenic
ACAATTTATGACTGCAGCTG+AGG 0.584213 4_2:+60020161 Msa0611690:CDS
GTCACAGGAACAGAAGAATA+CGG 0.587549 4_2:-60020243 None:intergenic
ATGGGAATGGGAATAGAATG+AGG 0.589702 4_2:-60019208 None:intergenic
TGTAGTAGTAATAGAAATGG+AGG 0.591912 4_2:+60020198 Msa0611690:CDS
TCCATGCTCCTCTTAAACGA+CGG 0.593486 4_2:+60019558 Msa0611690:CDS
AATACATGAATACATAGAAG+AGG 0.594019 4_2:+60021871 Msa0611690:three_prime_UTR
GCAGGAACAACACAACCCAT+TGG 0.594618 4_2:-60019360 None:intergenic
ATGATAGAGGAAATTAGCGA+TGG 0.597212 4_2:-60019424 None:intergenic
TGACAGAAAAGGGAGGAAGA+AGG 0.597320 4_2:+60020138 Msa0611690:intron
TGGCTACAGGATCCCGAGGA+GGG 0.600904 4_2:+60021673 Msa0611690:CDS
ACCAACTGAAGGTGATAGCA+TGG 0.601076 4_2:+60021701 Msa0611690:CDS
TCTTAATAAATTAGAAACAA+AGG 0.606924 4_2:+60020005 Msa0611690:CDS
GATCCTGTAGCCAAATGAAG+TGG 0.613037 4_2:-60021663 None:intergenic
TGAATGTGAAATTAAGGATG+CGG 0.615466 4_2:-60019105 None:intergenic
TACAACCCTACAATGCAGAA+AGG 0.616109 4_2:-60022320 None:intergenic
AAAAGTCATATTCACAGACA+TGG 0.616407 4_2:+60021759 Msa0611690:CDS
GATAATGTCACTGTTGTCAC+AGG 0.616977 4_2:-60020258 None:intergenic
AGCACGCATACTCGGCTGCA+CGG 0.618042 4_2:-60020424 None:intergenic
GTTCATCTTCGAGTCGAGAG+AGG 0.619371 4_2:-60019497 None:intergenic
GGATTGTGGAAAGATGATAG+AGG 0.619765 4_2:-60019437 None:intergenic
CAATACAACGACTCTTCCAA+AGG 0.620750 4_2:+60021609 Msa0611690:CDS
TCCATGCTATCACCTTCAGT+TGG 0.626142 4_2:-60021702 None:intergenic
TCCTGAGGTGAAGAGTCTGA+GGG 0.627907 4_2:+60020335 Msa0611690:CDS
GAGGGAAGGATTTGGTTCGA+GGG 0.630808 4_2:+60020353 Msa0611690:CDS
TTTATCTGCAAAATTCATGG+TGG 0.631088 4_2:-60021810 None:intergenic
GAGGTGAAGAGTCTGAGGGA+AGG 0.631423 4_2:+60020339 Msa0611690:CDS
TTGCAGATAAAAGTATACAT+GGG 0.633381 4_2:+60021822 Msa0611690:three_prime_UTR
AGAAGATAATGGATCCTCAA+CGG 0.637759 4_2:-60022408 None:intergenic
GAGTTTGGAGAGTTTATCGG+AGG 0.638169 4_2:-60019466 None:intergenic
CGGAGAGTTCCAGCTGCAAG+TGG 0.640628 4_2:+60021537 Msa0611690:CDS
TTTAAGAGGAGCATGGAGAG+AGG 0.641505 4_2:-60019552 None:intergenic
GGAGGGACATCACCAACTGA+AGG 0.643345 4_2:+60021690 Msa0611690:CDS
ACTTCATACTGCAACCGTTG+AGG 0.647866 4_2:+60022394 Msa0611690:three_prime_UTR
CTCTTCCAAAGGTAGCAGTA+CGG 0.648767 4_2:+60021620 Msa0611690:CDS
AGGGAAGGATTTGGTTCGAG+GGG 0.651342 4_2:+60020354 Msa0611690:CDS
CAGATAAAAGTATACATGGG+AGG 0.654402 4_2:+60021825 Msa0611690:three_prime_UTR
AGCAGACTTCTAGGAAACAG+AGG 0.657415 4_2:+60020463 Msa0611690:CDS
CATTTGGCTACAGGATCCCG+AGG 0.661979 4_2:+60021669 Msa0611690:CDS
ACCGTCGTTTAAGAGGAGCA+TGG 0.663203 4_2:-60019559 None:intergenic
AGAAATACCGACTTCATACG+CGG 0.668200 4_2:+60021517 Msa0611690:intron
ACCACGACAGGAAAACACAA+AGG 0.668802 4_2:-60022184 None:intergenic
ATCGAGAAACAGTTCTAACA+GGG 0.670598 4_2:+60020377 Msa0611690:CDS
GCCAAATGAAGTGGACTCTG+TGG 0.677464 4_2:-60021654 None:intergenic
TTGGCTACAGGATCCCGAGG+AGG 0.679289 4_2:+60021672 Msa0611690:CDS
TGTTTCCTAGAAGTCTGCTG+CGG 0.688682 4_2:-60020459 None:intergenic
TTGGTGATGACAGAAAAGGG+AGG 0.712344 4_2:+60020131 Msa0611690:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATGAGATTTATATAAAAT+GGG - chr4_2:60019229-60019248 None:intergenic 15.0%
!!! TTTTTTGTTTGATGAATTTT+GGG - chr4_2:60019336-60019355 None:intergenic 15.0%
!!! TTTTTTTGTTTGATGAATTT+TGG - chr4_2:60019337-60019356 None:intergenic 15.0%
!! TCTTAATAAATTAGAAACAA+AGG + chr4_2:60020005-60020024 Msa0611690:CDS 15.0%
!!! TTTTTTCTTGTCTAATTTTT+AGG - chr4_2:60020075-60020094 None:intergenic 15.0%
!! AATCTATAATATTTCCAAAT+TGG - chr4_2:60020676-60020695 None:intergenic 15.0%
!! ATTATAATCTGTCAAAAAAA+GGG + chr4_2:60022269-60022288 Msa0611690:three_prime_UTR 15.0%
!! GATTTATATAAAATGGGAAT+GGG - chr4_2:60019223-60019242 None:intergenic 20.0%
!! AGATTTATATAAAATGGGAA+TGG - chr4_2:60019224-60019243 None:intergenic 20.0%
!! GTGATGAGATTTATATAAAA+TGG - chr4_2:60019230-60019249 None:intergenic 20.0%
!! TTGAATAAAGTTATCAACTT+TGG + chr4_2:60019667-60019686 Msa0611690:intron 20.0%
!! ATTATAGATTAGTGAATTGA+TGG + chr4_2:60020686-60020705 Msa0611690:intron 20.0%
!! TAATAACATTACATCTTGTT+TGG + chr4_2:60021078-60021097 Msa0611690:intron 20.0%
!! CATTATAATCTGTCAAAAAA+AGG + chr4_2:60022268-60022287 Msa0611690:three_prime_UTR 20.0%
!! TTATAATCTGTCAAAAAAAG+GGG + chr4_2:60022270-60022289 Msa0611690:three_prime_UTR 20.0%
! TAGATGTGAATGTGAAATTA+AGG - chr4_2:60019114-60019133 None:intergenic 25.0%
! TAATAAGAAGAAGAAGAAGT+GGG - chr4_2:60019138-60019157 None:intergenic 25.0%
! ATAATAAGAAGAAGAAGAAG+TGG - chr4_2:60019139-60019158 None:intergenic 25.0%
! TCAAACAAAAAAAGATCCAA+TGG + chr4_2:60019344-60019363 Msa0611690:exon 25.0%
! CAAACAAAAAAAGATCCAAT+GGG + chr4_2:60019345-60019364 Msa0611690:exon 25.0%
!! TGATGATGATGATATTGAAA+GGG - chr4_2:60019592-60019611 None:intergenic 25.0%
!! ATGATGATGATGATATTGAA+AGG - chr4_2:60019593-60019612 None:intergenic 25.0%
!! ACCCTTTTGAATTTTTTATG+TGG + chr4_2:60019641-60019660 Msa0611690:intron 25.0%
! CCCACATAAAAAATTCAAAA+GGG - chr4_2:60019645-60019664 None:intergenic 25.0%
!! CCCTTTTGAATTTTTTATGT+GGG + chr4_2:60019642-60019661 Msa0611690:intron 25.0%
! ACCCACATAAAAAATTCAAA+AGG - chr4_2:60019646-60019665 None:intergenic 25.0%
!!! TTTGGAGTTTTTGATTCAAT+GGG + chr4_2:60019685-60019704 Msa0611690:intron 25.0%
!!! AAACTTTACTGTTTTTGTGA+TGG + chr4_2:60020564-60020583 Msa0611690:intron 25.0%
!!! ATGCTAGTCTTAAAAGTTTA+AGG + chr4_2:60020724-60020743 Msa0611690:intron 25.0%
! TGTTTAAATTTAGATCAGCA+AGG + chr4_2:60020828-60020847 Msa0611690:intron 25.0%
! ATATAGACAAGTCAATTTGT+AGG + chr4_2:60020870-60020889 Msa0611690:intron 25.0%
! GTTAAGACCAAAAAACATAT+AGG + chr4_2:60020997-60021016 Msa0611690:intron 25.0%
!! CAATTTTGCATACATAGTTT+TGG + chr4_2:60021048-60021067 Msa0611690:intron 25.0%
! CTTTATTAACCTTAGTTACT+AGG + chr4_2:60021255-60021274 Msa0611690:intron 25.0%
! TATAAACGATGTATACTAGA+TGG + chr4_2:60021360-60021379 Msa0611690:intron 25.0%
! TTGAGTTATCAAGTTTGAAT+TGG + chr4_2:60021462-60021481 Msa0611690:intron 25.0%
!! ACTTTTATCTGCAAAATTCA+TGG - chr4_2:60021816-60021835 None:intergenic 25.0%
!! TTTGCAGATAAAAGTATACA+TGG + chr4_2:60021821-60021840 Msa0611690:three_prime_UTR 25.0%
!! TTGCAGATAAAAGTATACAT+GGG + chr4_2:60021822-60021841 Msa0611690:three_prime_UTR 25.0%
! AATACATGAATACATAGAAG+AGG + chr4_2:60021871-60021890 Msa0611690:three_prime_UTR 25.0%
!! TTTAGTCCAATGTTATGTTT+TGG + chr4_2:60022065-60022084 Msa0611690:three_prime_UTR 25.0%
TGAATGTGAAATTAAGGATG+CGG - chr4_2:60019108-60019127 None:intergenic 30.0%
TTCTTGTTGAGGAGTTAAAA+GGG - chr4_2:60019272-60019291 None:intergenic 30.0%
ATTCTTGTTGAGGAGTTAAA+AGG - chr4_2:60019273-60019292 None:intergenic 30.0%
!!! CTTTGGAGTTTTTGATTCAA+TGG + chr4_2:60019684-60019703 Msa0611690:intron 30.0%
!! TTGGAGTTTTTGATTCAATG+GGG + chr4_2:60019686-60019705 Msa0611690:intron 30.0%
! TTTTGTGTATGCAGCAATTT+TGG + chr4_2:60019735-60019754 Msa0611690:intron 30.0%
! TAAAAATTCTGTGCCAGTTT+TGG + chr4_2:60019789-60019808 Msa0611690:CDS 30.0%
TGATCAATCTCTTTCTTCAA+AGG - chr4_2:60019826-60019845 None:intergenic 30.0%
TTGCAACAGTAATCTTAATG+AGG + chr4_2:60020095-60020114 Msa0611690:intron 30.0%
TGCAACAGTAATCTTAATGA+GGG + chr4_2:60020096-60020115 Msa0611690:intron 30.0%
!!! ATGAGGGTTTTGTTTTTGTT+TGG + chr4_2:60020112-60020131 Msa0611690:intron 30.0%
TACTACTACAAGGTTGAAAA+CGG - chr4_2:60020188-60020207 None:intergenic 30.0%
CCATTTCTATTACTACTACA+AGG - chr4_2:60020198-60020217 None:intergenic 30.0%
CCTTGTAGTAGTAATAGAAA+TGG + chr4_2:60020195-60020214 Msa0611690:CDS 30.0%
TGTAGTAGTAATAGAAATGG+AGG + chr4_2:60020198-60020217 Msa0611690:CDS 30.0%
!! ACACTCAATTTTGACCAATT+TGG + chr4_2:60020659-60020678 Msa0611690:intron 30.0%
TGATAATCCATAGTTTGGTT+GGG - chr4_2:60020950-60020969 None:intergenic 30.0%
!!! TGATAGGCCTATATGTTTTT+TGG - chr4_2:60021007-60021026 None:intergenic 30.0%
AATTGCAAAGGCAAATCATT+TGG - chr4_2:60021033-60021052 None:intergenic 30.0%
CTATGTATGCAAAATTGCAA+AGG - chr4_2:60021045-60021064 None:intergenic 30.0%
!! ATGAGTTCTCTAATTTGCTT+TGG - chr4_2:60021144-60021163 None:intergenic 30.0%
ACCAAAAACTTACTTGCAAA+TGG - chr4_2:60021203-60021222 None:intergenic 30.0%
TAATAAAGCATGCAGTTAAG+AGG - chr4_2:60021243-60021262 None:intergenic 30.0%
AAAAATCTGAGAGTTATAGC+TGG + chr4_2:60021736-60021755 Msa0611690:CDS 30.0%
AAAAGTCATATTCACAGACA+TGG + chr4_2:60021759-60021778 Msa0611690:CDS 30.0%
TTTATCTGCAAAATTCATGG+TGG - chr4_2:60021813-60021832 None:intergenic 30.0%
!!! AGGCTATTGGAATTTTTTGA+AGG + chr4_2:60021891-60021910 Msa0611690:three_prime_UTR 30.0%
GACTAAAAATATCTCTTCTG+GGG - chr4_2:60022052-60022071 None:intergenic 30.0%
GGACTAAAAATATCTCTTCT+GGG - chr4_2:60022053-60022072 None:intergenic 30.0%
TGGACTAAAAATATCTCTTC+TGG - chr4_2:60022054-60022073 None:intergenic 30.0%
!!! GTTATTTCTATTTTGTGCTC+TGG + chr4_2:60022241-60022260 Msa0611690:three_prime_UTR 30.0%
GTTCGATTTAGATAGAGTAT+CGG + chr4_2:60022357-60022376 Msa0611690:three_prime_UTR 30.0%
GTAGGATTAAGAGAAGATAA+TGG - chr4_2:60022422-60022441 None:intergenic 30.0%
TCACAAGTGATAAATAGAGT+AGG - chr4_2:60022440-60022459 None:intergenic 30.0%
TCTTGTTGAGGAGTTAAAAG+GGG - chr4_2:60019271-60019290 None:intergenic 35.0%
GAAGAAGAAGATTCTTGTTG+AGG - chr4_2:60019283-60019302 None:intergenic 35.0%
! TGAATTTTGGGCGTTAGATT+TGG - chr4_2:60019324-60019343 None:intergenic 35.0%
AAATCTAAACTGAGTTCTGC+AGG - chr4_2:60019381-60019400 None:intergenic 35.0%
! AGGAAATTAGCGATGGTTTT+GGG - chr4_2:60019420-60019439 None:intergenic 35.0%
ATGATAGAGGAAATTAGCGA+TGG - chr4_2:60019427-60019446 None:intergenic 35.0%
ATGGAGTTTGGAGAGTTTAT+CGG - chr4_2:60019472-60019491 None:intergenic 35.0%
!! TTTTGATTCAATGGGGCTAA+TGG + chr4_2:60019693-60019712 Msa0611690:intron 35.0%
TTTGATTCAATGGGGCTAAT+GGG + chr4_2:60019694-60019713 Msa0611690:intron 35.0%
!!! TGCAGCAATTTTGGTTTTGA+AGG + chr4_2:60019744-60019763 Msa0611690:intron 35.0%
!!! AGCAATTTTGGTTTTGAAGG+AGG + chr4_2:60019747-60019766 Msa0611690:intron 35.0%
!!! TTTGGTTTTGAAGGAGGAAT+TGG + chr4_2:60019753-60019772 Msa0611690:CDS 35.0%
GGAATAAACTCTTCCAAAAC+TGG - chr4_2:60019805-60019824 None:intergenic 35.0%
GCAGCAAAGATAAGAAGAAT+TGG + chr4_2:60019896-60019915 Msa0611690:CDS 35.0%
!! TTGTTTGGTGATGACAGAAA+AGG + chr4_2:60020127-60020146 Msa0611690:intron 35.0%
!! TGTTTGGTGATGACAGAAAA+GGG + chr4_2:60020128-60020147 Msa0611690:intron 35.0%
ACAGAAGAATACGGTGAAAA+AGG - chr4_2:60020237-60020256 None:intergenic 35.0%
! ATCGAGAAACAGTTCTAACA+GGG + chr4_2:60020377-60020396 Msa0611690:CDS 35.0%
TCTTATGTAGTTGTGTTGTG+AGG + chr4_2:60020629-60020648 Msa0611690:intron 35.0%
! TTTAGTTCACCAGTGAAGTT+AGG + chr4_2:60020802-60020821 Msa0611690:intron 35.0%
TTAGTTCACCAGTGAAGTTA+GGG + chr4_2:60020803-60020822 Msa0611690:intron 35.0%
TAAACAATCCCTAACTTCAC+TGG - chr4_2:60020814-60020833 None:intergenic 35.0%
AATCTGATCAAGGATTGTTC+CGG - chr4_2:60020919-60020938 None:intergenic 35.0%
GGAGGTTATTAATCTGATCA+AGG - chr4_2:60020929-60020948 None:intergenic 35.0%
CTGATAATCCATAGTTTGGT+TGG - chr4_2:60020951-60020970 None:intergenic 35.0%
!!! GTTTTTTGGTCTTAACCTTC+CGG - chr4_2:60020993-60021012 None:intergenic 35.0%
GCAAATCATTTGGACTTGAT+AGG - chr4_2:60021023-60021042 None:intergenic 35.0%
TCTTCACTTCATCATTAGTC+AGG - chr4_2:60021179-60021198 None:intergenic 35.0%
! GCCATTTGCAAGTAAGTTTT+TGG + chr4_2:60021199-60021218 Msa0611690:intron 35.0%
CATCAAACACCTAGTAACTA+AGG - chr4_2:60021267-60021286 None:intergenic 35.0%
AGTTACTAGGTGTTTGATGA+TGG + chr4_2:60021268-60021287 Msa0611690:intron 35.0%
TCATATCGTATGTGCATTCA+TGG + chr4_2:60021404-60021423 Msa0611690:intron 35.0%
! AGGCATTTAGGATTGAGATT+TGG + chr4_2:60021439-60021458 Msa0611690:intron 35.0%
TTGTAGTTCTTGGAGGTTTA+TGG + chr4_2:60021574-60021593 Msa0611690:CDS 35.0%
ATTCACAGACATGGAAAAGT+TGG + chr4_2:60021768-60021787 Msa0611690:CDS 35.0%
!! ACATGGAAAAGTTGGTGTAT+AGG + chr4_2:60021776-60021795 Msa0611690:CDS 35.0%
! CAGATAAAAGTATACATGGG+AGG + chr4_2:60021825-60021844 Msa0611690:three_prime_UTR 35.0%
GAATACATAGAAGAGGCTAT+TGG + chr4_2:60021878-60021897 Msa0611690:three_prime_UTR 35.0%
TGATTCTTATCGTGGAATGT+AGG - chr4_2:60021977-60021996 None:intergenic 35.0%
TACATTCCACGATAAGAATC+AGG + chr4_2:60021976-60021995 Msa0611690:three_prime_UTR 35.0%
AAATGACCTGATTCTTATCG+TGG - chr4_2:60021985-60022004 None:intergenic 35.0%
!! AGTCCAATGTTATGTTTTGG+TGG + chr4_2:60022068-60022087 Msa0611690:three_prime_UTR 35.0%
CATCCACCAAAACATAACAT+TGG - chr4_2:60022074-60022093 None:intergenic 35.0%
!! CATCTCTGAAATTTGTGAAG+TGG - chr4_2:60022130-60022149 None:intergenic 35.0%
! TTTTCTCAAAGACACTGAAC+AGG + chr4_2:60022208-60022227 Msa0611690:three_prime_UTR 35.0%
TGTCAAAAAAAGGGGCTTTA+AGG + chr4_2:60022278-60022297 Msa0611690:three_prime_UTR 35.0%
! GTCAAAAAAAGGGGCTTTAA+GGG + chr4_2:60022279-60022298 Msa0611690:three_prime_UTR 35.0%
TCTTTCCTTTCTGCATTGTA+GGG + chr4_2:60022315-60022334 Msa0611690:three_prime_UTR 35.0%
!! AGAAGATAATGGATCCTCAA+CGG - chr4_2:60022411-60022430 None:intergenic 35.0%
ATGGGAATGGGAATAGAATG+AGG - chr4_2:60019211-60019230 None:intergenic 40.0%
!!! ATGGTTTTGGGAACGAAAGT+TGG - chr4_2:60019408-60019427 None:intergenic 40.0%
! GAGGAAATTAGCGATGGTTT+TGG - chr4_2:60019421-60019440 None:intergenic 40.0%
GGATTGTGGAAAGATGATAG+AGG - chr4_2:60019440-60019459 None:intergenic 40.0%
AGAGTTTATCGGAGGTTGTT+TGG - chr4_2:60019461-60019480 None:intergenic 40.0%
AGAGAGGAAATCATGGAGTT+TGG - chr4_2:60019484-60019503 None:intergenic 40.0%
GGATGAGTTCTGTTCAGTTA+TGG + chr4_2:60019917-60019936 Msa0611690:CDS 40.0%
ACAATTTATGACTGCAGCTG+AGG + chr4_2:60020161-60020180 Msa0611690:CDS 40.0%
GTCACAGGAACAGAAGAATA+CGG - chr4_2:60020246-60020265 None:intergenic 40.0%
GATAATGTCACTGTTGTCAC+AGG - chr4_2:60020261-60020280 None:intergenic 40.0%
ATCAGCACCTAAAGAAGAGA+AGG + chr4_2:60020278-60020297 Msa0611690:CDS 40.0%
GGTTCTTCCTTCTCTTCTTT+AGG - chr4_2:60020288-60020307 None:intergenic 40.0%
AAGAGAAGGAAGAACCTGTA+AGG + chr4_2:60020292-60020311 Msa0611690:CDS 40.0%
AGGAAGAACCTGTAAGGAAT+AGG + chr4_2:60020298-60020317 Msa0611690:CDS 40.0%
AAAGAGAGCCTATTCCTTAC+AGG - chr4_2:60020309-60020328 None:intergenic 40.0%
! GATCGAGAAACAGTTCTAAC+AGG + chr4_2:60020376-60020395 Msa0611690:CDS 40.0%
GCAACGCATTAACAAATCTC+CGG - chr4_2:60020512-60020531 None:intergenic 40.0%
! TAGTGAATTGATGGATGTGC+TGG + chr4_2:60020695-60020714 Msa0611690:intron 40.0%
AGATCAGCAAGGAGACAAAT+AGG + chr4_2:60020839-60020858 Msa0611690:intron 40.0%
TCTACCCCGAATTCAAATCT+TGG + chr4_2:60020893-60020912 Msa0611690:intron 40.0%
TTCCGGCCAAGATTTGAATT+CGG - chr4_2:60020902-60020921 None:intergenic 40.0%
ATAACCTCCCAACCAAACTA+TGG + chr4_2:60020940-60020959 Msa0611690:intron 40.0%
TAATCCATAGTTTGGTTGGG+AGG - chr4_2:60020947-60020966 None:intergenic 40.0%
GGGACTGATAATCCATAGTT+TGG - chr4_2:60020955-60020974 None:intergenic 40.0%
! AGAGAACTCATGCAAGTTGA+TGG + chr4_2:60021153-60021172 Msa0611690:CDS 40.0%
ATTCATGGTGTCTGGTTATG+AGG + chr4_2:60021419-60021438 Msa0611690:intron 40.0%
! TGTCTGGTTATGAGGCATTT+AGG + chr4_2:60021427-60021446 Msa0611690:intron 40.0%
!! AGAAATACCGACTTCATACG+CGG + chr4_2:60021517-60021536 Msa0611690:intron 40.0%
GATCAGTCTGTTGTAGTTCT+TGG + chr4_2:60021564-60021583 Msa0611690:CDS 40.0%
GAGTCGTTGTATTGTTCTTG+AGG - chr4_2:60021603-60021622 None:intergenic 40.0%
CAATACAACGACTCTTCCAA+AGG + chr4_2:60021609-60021628 Msa0611690:CDS 40.0%
! CTTTGAGAAAAAACCACGAC+AGG - chr4_2:60022199-60022218 None:intergenic 40.0%
CTCTTTCCTTTCTGCATTGT+AGG + chr4_2:60022314-60022333 Msa0611690:three_prime_UTR 40.0%
TACAACCCTACAATGCAGAA+AGG - chr4_2:60022323-60022342 None:intergenic 40.0%
TAAATCGAACAGTCCCTACA+AGG - chr4_2:60022347-60022366 None:intergenic 40.0%
!! AGAGTATCGGTTCTCATAGT+AGG + chr4_2:60022370-60022389 Msa0611690:three_prime_UTR 40.0%
AGAGAAAGCAACGACGAATG+AGG - chr4_2:60019037-60019056 None:intergenic 45.0%
ATCGGAGGTTGTTTGGATTG+TGG - chr4_2:60019454-60019473 None:intergenic 45.0%
GAGTTTGGAGAGTTTATCGG+AGG - chr4_2:60019469-60019488 None:intergenic 45.0%
! TTTAAGAGGAGCATGGAGAG+AGG - chr4_2:60019555-60019574 None:intergenic 45.0%
TCCATGCTCCTCTTAAACGA+CGG + chr4_2:60019558-60019577 Msa0611690:CDS 45.0%
AGTACGAACCGTCGTTTAAG+AGG - chr4_2:60019569-60019588 None:intergenic 45.0%
!! TTGGTGATGACAGAAAAGGG+AGG + chr4_2:60020131-60020150 Msa0611690:intron 45.0%
TGACAGAAAAGGGAGGAAGA+AGG + chr4_2:60020138-60020157 Msa0611690:intron 45.0%
AAGAGTCTGAGGGAAGGATT+TGG + chr4_2:60020345-60020364 Msa0611690:CDS 45.0%
TGTTTCCTAGAAGTCTGCTG+CGG - chr4_2:60020462-60020481 None:intergenic 45.0%
! AGCAGACTTCTAGGAAACAG+AGG + chr4_2:60020463-60020482 Msa0611690:CDS 45.0%
ACAAATCTCCGGTGCAATTC+CGG - chr4_2:60020501-60020520 None:intergenic 45.0%
GTTAATGCGTTGCAGAAGCT+TGG + chr4_2:60020519-60020538 Msa0611690:CDS 45.0%
TCCGGCCAAGATTTGAATTC+GGG - chr4_2:60020901-60020920 None:intergenic 45.0%
TCTTAACCTTCCGGTTCCTA+GGG - chr4_2:60020984-60021003 None:intergenic 45.0%
GTATGTGCATTCATGGTGTC+TGG + chr4_2:60021411-60021430 Msa0611690:intron 45.0%
CAGTCTGTTGTAGTTCTTGG+AGG + chr4_2:60021567-60021586 Msa0611690:CDS 45.0%
CTCTTCCAAAGGTAGCAGTA+CGG + chr4_2:60021620-60021639 Msa0611690:CDS 45.0%
TCCACAGAGTCCACTTCATT+TGG + chr4_2:60021653-60021672 Msa0611690:CDS 45.0%
AGTCCACTTCATTTGGCTAC+AGG + chr4_2:60021660-60021679 Msa0611690:CDS 45.0%
GATCCTGTAGCCAAATGAAG+TGG - chr4_2:60021666-60021685 None:intergenic 45.0%
ACCAACTGAAGGTGATAGCA+TGG + chr4_2:60021701-60021720 Msa0611690:CDS 45.0%
TCCATGCTATCACCTTCAGT+TGG - chr4_2:60021705-60021724 None:intergenic 45.0%
! ACCTTTGTGTTTTCCTGTCG+TGG + chr4_2:60022183-60022202 Msa0611690:three_prime_UTR 45.0%
ACCACGACAGGAAAACACAA+AGG - chr4_2:60022187-60022206 None:intergenic 45.0%
!! TTGTAGGGTTGTAGCCTTGT+AGG + chr4_2:60022330-60022349 Msa0611690:three_prime_UTR 45.0%
!! TGTAGGGTTGTAGCCTTGTA+GGG + chr4_2:60022331-60022350 Msa0611690:three_prime_UTR 45.0%
ACTTCATACTGCAACCGTTG+AGG + chr4_2:60022394-60022413 Msa0611690:three_prime_UTR 45.0%
GCAGGAACAACACAACCCAT+TGG - chr4_2:60019363-60019382 None:intergenic 50.0%
CGAGTCGAGAGAGGAAATCA+TGG - chr4_2:60019491-60019510 None:intergenic 50.0%
GTTCATCTTCGAGTCGAGAG+AGG - chr4_2:60019500-60019519 None:intergenic 50.0%
ACCGTCGTTTAAGAGGAGCA+TGG - chr4_2:60019562-60019581 None:intergenic 50.0%
! GCTCTCTTTTCTGACTCCTG+AGG + chr4_2:60020320-60020339 Msa0611690:CDS 50.0%
TCCTGAGGTGAAGAGTCTGA+GGG + chr4_2:60020335-60020354 Msa0611690:CDS 50.0%
! TGAGGGAAGGATTTGGTTCG+AGG + chr4_2:60020352-60020371 Msa0611690:CDS 50.0%
! GAGGGAAGGATTTGGTTCGA+GGG + chr4_2:60020353-60020372 Msa0611690:CDS 50.0%
! AGGGAAGGATTTGGTTCGAG+GGG + chr4_2:60020354-60020373 Msa0611690:CDS 50.0%
CTAGGAAACAGAGGAGATGC+TGG + chr4_2:60020472-60020491 Msa0611690:CDS 50.0%
AATGCGTTGCAGAAGCTTGG+AGG + chr4_2:60020522-60020541 Msa0611690:CDS 50.0%
CAGAAGCTTGGAGGTTCTCA+AGG + chr4_2:60020531-60020550 Msa0611690:CDS 50.0%
CCGGCCAAGATTTGAATTCG+GGG - chr4_2:60020900-60020919 None:intergenic 50.0%
CCCCGAATTCAAATCTTGGC+CGG + chr4_2:60020897-60020916 Msa0611690:intron 50.0%
TATCAGTCCCTCCTTTCCCT+AGG + chr4_2:60020965-60020984 Msa0611690:intron 50.0%
GTCTTAACCTTCCGGTTCCT+AGG - chr4_2:60020985-60021004 None:intergenic 50.0%
GAACTCTCCGCGTATGAAGT+CGG - chr4_2:60021527-60021546 None:intergenic 50.0%
TGATCAGTACCACTTGCAGC+TGG - chr4_2:60021549-60021568 None:intergenic 50.0%
! GCCAAATGAAGTGGACTCTG+TGG - chr4_2:60021657-60021676 None:intergenic 50.0%
CTCCTGAGGTGAAGAGTCTG+AGG + chr4_2:60020334-60020353 Msa0611690:CDS 55.0%
TCCCTCAGACTCTTCACCTC+AGG - chr4_2:60020339-60020358 None:intergenic 55.0%
GAGGTGAAGAGTCTGAGGGA+AGG + chr4_2:60020339-60020358 Msa0611690:CDS 55.0%
TTCAGCCGCAGCAGACTTCT+AGG + chr4_2:60020454-60020473 Msa0611690:CDS 55.0%
ACAGAGGAGATGCTGGTCAC+CGG + chr4_2:60020479-60020498 Msa0611690:CDS 55.0%
TCCCTCCTTTCCCTAGGAAC+CGG + chr4_2:60020971-60020990 Msa0611690:intron 55.0%
TCCGGTTCCTAGGGAAAGGA+GGG - chr4_2:60020975-60020994 None:intergenic 55.0%
TTCCGGTTCCTAGGGAAAGG+AGG - chr4_2:60020976-60020995 None:intergenic 55.0%
TCCTTTCCCTAGGAACCGGA+AGG + chr4_2:60020975-60020994 Msa0611690:intron 55.0%
ACCTTCCGGTTCCTAGGGAA+AGG - chr4_2:60020979-60020998 None:intergenic 55.0%
AGACGCCGTACTGCTACCTT+TGG - chr4_2:60021628-60021647 None:intergenic 55.0%
AAAGGTAGCAGTACGGCGTC+TGG + chr4_2:60021627-60021646 Msa0611690:CDS 55.0%
CATTTGGCTACAGGATCCCG+AGG + chr4_2:60021669-60021688 Msa0611690:CDS 55.0%
!! AGTTGGTGATGTCCCTCCTC+GGG - chr4_2:60021688-60021707 None:intergenic 55.0%
!! CAGTTGGTGATGTCCCTCCT+CGG - chr4_2:60021689-60021708 None:intergenic 55.0%
GGAGGGACATCACCAACTGA+AGG + chr4_2:60021690-60021709 Msa0611690:CDS 55.0%
ATACTCGGCTGCACGGTTGG+AGG - chr4_2:60020420-60020439 None:intergenic 60.0%
AGCACGCATACTCGGCTGCA+CGG - chr4_2:60020427-60020446 None:intergenic 60.0%
GAAGTCTGCTGCGGCTGAAG+CGG - chr4_2:60020453-60020472 None:intergenic 60.0%
GCTGGTCACCGGAATTGCAC+CGG + chr4_2:60020490-60020509 Msa0611690:CDS 60.0%
CGGAGAGTTCCAGCTGCAAG+TGG + chr4_2:60021537-60021556 Msa0611690:CDS 60.0%
TTGGCTACAGGATCCCGAGG+AGG + chr4_2:60021672-60021691 Msa0611690:CDS 60.0%
TGGCTACAGGATCCCGAGGA+GGG + chr4_2:60021673-60021692 Msa0611690:CDS 60.0%
CGCATACTCGGCTGCACGGT+TGG - chr4_2:60020423-60020442 None:intergenic 65.0%
AGCGGCGCAGCACGCATACT+CGG - chr4_2:60020435-60020454 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 60019027 60022466 60019027 ID=Msa0611690;Name=Msa0611690
chr4_2 mRNA 60019027 60022466 60019027 ID=Msa0611690-mRNA-1;Parent=Msa0611690;Name=Msa0611690-mRNA-1;_AED=0.11;_eAED=0.11;_QI=336|0.75|0.8|1|1|1|5|669|419
chr4_2 exon 60019027 60019579 60019027 ID=Msa0611690-mRNA-1:exon:16037;Parent=Msa0611690-mRNA-1
chr4_2 exon 60019749 60020026 60019749 ID=Msa0611690-mRNA-1:exon:16038;Parent=Msa0611690-mRNA-1
chr4_2 exon 60020144 60020552 60020144 ID=Msa0611690-mRNA-1:exon:16039;Parent=Msa0611690-mRNA-1
chr4_2 exon 60021133 60021209 60021133 ID=Msa0611690-mRNA-1:exon:16040;Parent=Msa0611690-mRNA-1
chr4_2 exon 60021519 60022466 60021519 ID=Msa0611690-mRNA-1:exon:16041;Parent=Msa0611690-mRNA-1
chr4_2 five_prime_UTR 60019027 60019362 60019027 ID=Msa0611690-mRNA-1:five_prime_utr;Parent=Msa0611690-mRNA-1
chr4_2 CDS 60019363 60019579 60019363 ID=Msa0611690-mRNA-1:cds;Parent=Msa0611690-mRNA-1
chr4_2 CDS 60019749 60020026 60019749 ID=Msa0611690-mRNA-1:cds;Parent=Msa0611690-mRNA-1
chr4_2 CDS 60020144 60020552 60020144 ID=Msa0611690-mRNA-1:cds;Parent=Msa0611690-mRNA-1
chr4_2 CDS 60021133 60021209 60021133 ID=Msa0611690-mRNA-1:cds;Parent=Msa0611690-mRNA-1
chr4_2 CDS 60021519 60021797 60021519 ID=Msa0611690-mRNA-1:cds;Parent=Msa0611690-mRNA-1
chr4_2 three_prime_UTR 60021798 60022466 60021798 ID=Msa0611690-mRNA-1:three_prime_utr;Parent=Msa0611690-mRNA-1
Gene Sequence

>Msa0611690

ATGGGTTGTGTTGTTCCTGCAGAACTCAGTTTAGATTTTAGACCAACTTTCGTTCCCAAAACCATCGCTAATTTCCTCTATCATCTTTCCACAATCCAAACAACCTCCGATAAACTCTCCAAACTCCATGATTTCCTCTCTCGACTCGAAGATGAACTCAATAAGATCGATGCTTTCAAACGTGAACTACCTCTCTCCATGCTCCTCTTAAACGACGCAATTTTGGTTTTGAAGGAGGAATTGGAAAAATGTAGAAGTAAAAATTCTGTGCCAGTTTTGGAAGAGTTTATTCCTTTGAAGAAAGAGATTGATCAGAGTGAAGAGAATAATAATAATGATAGAGACAAGAACAACAACAATGAATGCAGCAAAGATAAGAAGAATTGGATGAGTTCTGTTCAGTTATGGAATAACAACACAACTACTTCTAATAATAATGTCTCTGATCATCATCATAAACATAACAATCATAATCTTAATAAATTAGAAACAAAGAAAAGGGAGGAAGAAGGACAATTTATGACTGCAGCTGAGGACCGTTTTCAACCTTGTAGTAGTAATAGAAATGGAGGAAGTGCTTTTATGCCTTTTTCACCGTATTCTTCTGTTCCTGTGACAACAGTGACATTATCAGCACCTAAAGAAGAGAAGGAAGAACCTGTAAGGAATAGGCTCTCTTTTCTGACTCCTGAGGTGAAGAGTCTGAGGGAAGGATTTGGTTCGAGGGGATCGAGAAACAGTTCTAACAGGGCAGTTTCGTCGTCGTCTCCTCCAACCGTGCAGCCGAGTATGCGTGCTGCGCCGCTTCAGCCGCAGCAGACTTCTAGGAAACAGAGGAGATGCTGGTCACCGGAATTGCACCGGAGATTTGTTAATGCGTTGCAGAAGCTTGGAGGTTCTCAAGCAGCAACTCCAAAGCAAATTAGAGAACTCATGCAAGTTGATGGCCTGACTAATGATGAAGTGAAGAGCCATTTGCAAAAATACCGACTTCATACGCGGAGAGTTCCAGCTGCAAGTGGTACTGATCAGTCTGTTGTAGTTCTTGGAGGTTTATGGATGCCTCAAGAACAATACAACGACTCTTCCAAAGGTAGCAGTACGGCGTCTGGTTCTCCACAGAGTCCACTTCATTTGGCTACAGGATCCCGAGGAGGGACATCACCAACTGAAGGTGATAGCATGGACGATGATGATGAAAAATCTGAGAGTTATAGCTGGAAAAGTCATATTCACAGACATGGAAAAGTTGGTGTATAG
Protein sequence

>Msa0611690

MGCVVPAELSLDFRPTFVPKTIANFLYHLSTIQTTSDKLSKLHDFLSRLEDELNKIDAFKRELPLSMLLLNDAILVLKEELEKCRSKNSVPVLEEFIPLKKEIDQSEENNNNDRDKNNNNECSKDKKNWMSSVQLWNNNTTTSNNNVSDHHHKHNNHNLNKLETKKREEEGQFMTAAEDRFQPCSSNRNGGSAFMPFSPYSSVPVTTVTLSAPKEEKEEPVRNRLSFLTPEVKSLREGFGSRGSRNSSNRAVSSSSPPTVQPSMRAAPLQPQQTSRKQRRCWSPELHRRFVNALQKLGGSQAATPKQIRELMQVDGLTNDEVKSHLQKYRLHTRRVPAASGTDQSVVVLGGLWMPQEQYNDSSKGSSTASGSPQSPLHLATGSRGGTSPTEGDSMDDDDEKSESYSWKSHIHRHGKVGV*