Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | XP_013457087.1 | 95.973 | 298 | 7 | 2 | 1 | 293 | 1 | 298 | 0.0 | 587 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | sp|Q9FZA4|DOF14_ARATH | 81.356 | 59 | 10 | 1 | 50 | 107 | 27 | 85 | 1.65e-28 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | A0A072UPW6 | 95.973 | 298 | 7 | 2 | 1 | 293 | 1 | 298 | 0.0 | 587 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0612970 | TF | C2C2-Dof |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | MtrunA17_Chr4g0047761 | 95.804 | 286 | 7 | 2 | 13 | 293 | 35 | 320 | 0.0 | 564 |
| Msa0612970 | MtrunA17_Chr2g0282051 | 58.689 | 305 | 83 | 14 | 1 | 293 | 1 | 274 | 8.30e-97 | 286 |
| Msa0612970 | MtrunA17_Chr2g0328021 | 41.989 | 181 | 82 | 6 | 50 | 227 | 62 | 222 | 3.29e-31 | 117 |
| Msa0612970 | MtrunA17_Chr4g0047001 | 54.472 | 123 | 45 | 3 | 17 | 132 | 16 | 134 | 1.38e-30 | 118 |
| Msa0612970 | MtrunA17_Chr3g0127201 | 67.105 | 76 | 20 | 2 | 29 | 103 | 10 | 81 | 3.72e-29 | 112 |
| Msa0612970 | MtrunA17_Chr8g0374251 | 59.259 | 81 | 32 | 1 | 48 | 127 | 66 | 146 | 8.01e-29 | 112 |
| Msa0612970 | MtrunA17_Chr8g0374111 | 60.256 | 78 | 30 | 1 | 46 | 122 | 25 | 102 | 9.00e-29 | 110 |
| Msa0612970 | MtrunA17_Chr2g0326001 | 31.923 | 260 | 144 | 10 | 36 | 269 | 25 | 277 | 1.36e-27 | 108 |
| Msa0612970 | MtrunA17_Chr8g0346551 | 55.422 | 83 | 36 | 1 | 37 | 118 | 11 | 93 | 2.65e-27 | 107 |
| Msa0612970 | MtrunA17_Chr3g0126201 | 55.556 | 99 | 42 | 2 | 9 | 105 | 9 | 107 | 1.32e-26 | 106 |
| Msa0612970 | MtrunA17_Chr2g0282511 | 85.714 | 49 | 7 | 0 | 50 | 98 | 45 | 93 | 1.70e-26 | 105 |
| Msa0612970 | MtrunA17_Chr8g0367161 | 64.179 | 67 | 23 | 1 | 45 | 110 | 27 | 93 | 2.11e-26 | 106 |
| Msa0612970 | MtrunA17_Chr1g0177251 | 52.174 | 92 | 43 | 1 | 51 | 141 | 13 | 104 | 4.17e-26 | 104 |
| Msa0612970 | MtrunA17_Chr8g0341671 | 74.545 | 55 | 14 | 0 | 46 | 100 | 16 | 70 | 4.36e-26 | 102 |
| Msa0612970 | MtrunA17_Chr7g0223311 | 80.357 | 56 | 9 | 1 | 52 | 105 | 79 | 134 | 4.98e-26 | 105 |
| Msa0612970 | MtrunA17_Chr7g0237921 | 81.132 | 53 | 9 | 1 | 52 | 103 | 78 | 130 | 5.87e-26 | 105 |
| Msa0612970 | MtrunA17_Chr4g0031411 | 58.228 | 79 | 31 | 1 | 50 | 126 | 75 | 153 | 6.08e-26 | 104 |
| Msa0612970 | MtrunA17_Chr4g0010151 | 76.364 | 55 | 12 | 1 | 50 | 103 | 82 | 136 | 7.55e-26 | 105 |
| Msa0612970 | MtrunA17_Chr4g0030051 | 69.697 | 66 | 19 | 1 | 37 | 101 | 28 | 93 | 7.63e-26 | 103 |
| Msa0612970 | MtrunA17_Chr4g0061761 | 61.333 | 75 | 29 | 0 | 48 | 122 | 38 | 112 | 2.57e-25 | 102 |
| Msa0612970 | MtrunA17_Chr2g0308011 | 81.633 | 49 | 9 | 0 | 50 | 98 | 37 | 85 | 3.76e-25 | 100 |
| Msa0612970 | MtrunA17_Chr1g0188851 | 60.606 | 66 | 25 | 1 | 39 | 103 | 32 | 97 | 4.09e-25 | 101 |
| Msa0612970 | MtrunA17_Chr3g0117601 | 48.624 | 109 | 45 | 4 | 1 | 98 | 1 | 109 | 4.70e-24 | 99.8 |
| Msa0612970 | MtrunA17_Chr5g0411951 | 72.549 | 51 | 14 | 0 | 48 | 98 | 41 | 91 | 6.83e-24 | 99.4 |
| Msa0612970 | MtrunA17_Chr3g0092111 | 58.462 | 65 | 27 | 0 | 39 | 103 | 125 | 189 | 3.60e-23 | 98.6 |
| Msa0612970 | MtrunA17_Chr7g0217581 | 50.000 | 92 | 39 | 2 | 52 | 136 | 234 | 325 | 6.86e-23 | 98.6 |
| Msa0612970 | MtrunA17_Chr6g0460721 | 42.241 | 116 | 51 | 2 | 7 | 121 | 61 | 161 | 8.33e-23 | 96.3 |
| Msa0612970 | MtrunA17_Chr2g0283811 | 67.308 | 52 | 17 | 0 | 52 | 103 | 49 | 100 | 8.51e-23 | 92.4 |
| Msa0612970 | MtrunA17_Chr2g0282441 | 61.905 | 63 | 24 | 0 | 36 | 98 | 11 | 73 | 8.88e-23 | 96.3 |
| Msa0612970 | MtrunA17_Chr4g0042481 | 70.370 | 54 | 15 | 1 | 52 | 104 | 124 | 177 | 1.57e-22 | 97.1 |
| Msa0612970 | MtrunA17_Chr7g0254161 | 56.164 | 73 | 31 | 1 | 50 | 121 | 101 | 173 | 2.07e-22 | 96.3 |
| Msa0612970 | MtrunA17_Chr6g0460731 | 54.545 | 77 | 34 | 1 | 50 | 125 | 96 | 172 | 2.14e-22 | 95.5 |
| Msa0612970 | MtrunA17_Chr6g0453321 | 66.667 | 54 | 18 | 0 | 50 | 103 | 136 | 189 | 2.31e-22 | 96.7 |
| Msa0612970 | MtrunA17_Chr5g0417561 | 41.593 | 113 | 63 | 2 | 38 | 148 | 105 | 216 | 5.86e-22 | 94.4 |
| Msa0612970 | MtrunA17_Chr5g0417551 | 42.593 | 108 | 59 | 2 | 38 | 143 | 113 | 219 | 6.62e-22 | 94.4 |
| Msa0612970 | MtrunA17_Chr5g0417571 | 53.030 | 66 | 31 | 0 | 39 | 104 | 127 | 192 | 7.95e-22 | 93.2 |
| Msa0612970 | MtrunA17_Chr8g0355261 | 65.455 | 55 | 18 | 1 | 50 | 103 | 118 | 172 | 1.48e-21 | 94.0 |
| Msa0612970 | MtrunA17_Chr5g0417601 | 53.030 | 66 | 31 | 0 | 39 | 104 | 129 | 194 | 2.95e-20 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | AT1G28310.1 | 81.356 | 59 | 10 | 1 | 50 | 107 | 27 | 85 | 1.68e-29 | 114 |
| Msa0612970 | AT3G55370.1 | 41.081 | 185 | 90 | 6 | 50 | 227 | 76 | 248 | 2.21e-29 | 114 |
| Msa0612970 | AT1G28310.2 | 81.356 | 59 | 10 | 1 | 50 | 107 | 51 | 109 | 2.54e-29 | 114 |
| Msa0612970 | AT3G55370.2 | 41.081 | 185 | 90 | 6 | 50 | 227 | 76 | 248 | 2.61e-29 | 114 |
| Msa0612970 | AT3G55370.3 | 41.081 | 185 | 90 | 6 | 50 | 227 | 121 | 293 | 3.95e-29 | 114 |
| Msa0612970 | AT5G60850.1 | 69.863 | 73 | 21 | 1 | 50 | 121 | 51 | 123 | 1.85e-28 | 111 |
| Msa0612970 | AT1G51700.1 | 60.274 | 73 | 29 | 0 | 38 | 110 | 21 | 93 | 6.77e-28 | 107 |
| Msa0612970 | AT5G66940.1 | 51.546 | 97 | 45 | 2 | 23 | 119 | 6 | 100 | 2.21e-27 | 106 |
| Msa0612970 | AT5G60200.1 | 80.000 | 55 | 10 | 1 | 50 | 103 | 55 | 109 | 4.64e-27 | 106 |
| Msa0612970 | AT5G65590.1 | 53.933 | 89 | 34 | 2 | 28 | 109 | 13 | 101 | 4.72e-27 | 107 |
| Msa0612970 | AT3G50410.1 | 72.581 | 62 | 16 | 1 | 46 | 106 | 26 | 87 | 5.36e-27 | 106 |
| Msa0612970 | AT1G07640.2 | 48.246 | 114 | 43 | 3 | 8 | 105 | 21 | 134 | 8.67e-27 | 107 |
| Msa0612970 | AT1G07640.3 | 48.246 | 114 | 43 | 3 | 8 | 105 | 29 | 142 | 1.01e-26 | 107 |
| Msa0612970 | AT1G07640.1 | 60.811 | 74 | 27 | 1 | 34 | 105 | 5 | 78 | 1.93e-26 | 105 |
| Msa0612970 | AT3G21270.1 | 73.684 | 57 | 15 | 0 | 50 | 106 | 29 | 85 | 3.74e-26 | 102 |
| Msa0612970 | AT2G28510.1 | 69.841 | 63 | 18 | 1 | 42 | 103 | 40 | 102 | 3.85e-26 | 104 |
| Msa0612970 | AT3G61850.2 | 70.492 | 61 | 17 | 1 | 48 | 107 | 60 | 120 | 7.13e-26 | 103 |
| Msa0612970 | AT3G61850.3 | 70.492 | 61 | 17 | 1 | 48 | 107 | 60 | 120 | 7.13e-26 | 103 |
| Msa0612970 | AT3G61850.1 | 70.492 | 61 | 17 | 1 | 48 | 107 | 72 | 132 | 7.38e-26 | 104 |
| Msa0612970 | AT3G61850.5 | 70.492 | 61 | 17 | 1 | 48 | 107 | 60 | 120 | 1.01e-25 | 104 |
| Msa0612970 | AT2G28810.2 | 31.970 | 269 | 144 | 6 | 36 | 280 | 63 | 316 | 1.03e-25 | 104 |
| Msa0612970 | AT2G28810.1 | 66.667 | 63 | 21 | 0 | 36 | 98 | 80 | 142 | 1.14e-25 | 104 |
| Msa0612970 | AT3G61850.4 | 70.492 | 61 | 17 | 1 | 48 | 107 | 72 | 132 | 1.25e-25 | 104 |
| Msa0612970 | AT3G45610.1 | 71.930 | 57 | 15 | 1 | 48 | 103 | 38 | 94 | 1.38e-25 | 102 |
| Msa0612970 | AT2G37590.1 | 57.534 | 73 | 30 | 1 | 34 | 105 | 73 | 145 | 2.06e-25 | 103 |
| Msa0612970 | AT4G24060.1 | 62.121 | 66 | 24 | 1 | 39 | 103 | 42 | 107 | 3.84e-25 | 103 |
| Msa0612970 | AT5G02460.1 | 76.364 | 55 | 12 | 1 | 50 | 103 | 95 | 149 | 5.66e-25 | 103 |
| Msa0612970 | AT4G38000.1 | 71.698 | 53 | 15 | 0 | 50 | 102 | 41 | 93 | 1.93e-24 | 99.4 |
| Msa0612970 | AT5G62430.1 | 47.872 | 94 | 46 | 1 | 19 | 109 | 20 | 113 | 2.46e-24 | 100 |
| Msa0612970 | AT1G47655.1 | 73.077 | 52 | 14 | 0 | 47 | 98 | 26 | 77 | 7.09e-24 | 97.1 |
| Msa0612970 | AT3G52440.1 | 72.727 | 55 | 14 | 1 | 52 | 105 | 27 | 81 | 7.45e-24 | 97.8 |
| Msa0612970 | AT2G46590.1 | 76.000 | 50 | 12 | 0 | 48 | 97 | 66 | 115 | 8.48e-24 | 99.8 |
| Msa0612970 | AT2G46590.2 | 76.000 | 50 | 12 | 0 | 48 | 97 | 78 | 127 | 9.32e-24 | 99.8 |
| Msa0612970 | AT3G52440.2 | 72.727 | 55 | 14 | 1 | 52 | 105 | 46 | 100 | 1.03e-23 | 97.8 |
| Msa0612970 | AT4G21050.1 | 58.209 | 67 | 27 | 1 | 52 | 117 | 26 | 92 | 1.21e-23 | 96.3 |
| Msa0612970 | AT1G21340.1 | 69.643 | 56 | 16 | 1 | 52 | 106 | 40 | 95 | 1.24e-23 | 97.4 |
| Msa0612970 | AT4G00940.1 | 67.213 | 61 | 19 | 1 | 48 | 107 | 66 | 126 | 1.25e-23 | 98.2 |
| Msa0612970 | AT4G00940.3 | 67.213 | 61 | 19 | 1 | 48 | 107 | 66 | 126 | 1.25e-23 | 98.2 |
| Msa0612970 | AT4G00940.2 | 67.213 | 61 | 19 | 1 | 48 | 107 | 66 | 126 | 1.25e-23 | 98.2 |
| Msa0612970 | AT3G47500.1 | 70.370 | 54 | 16 | 0 | 50 | 103 | 110 | 163 | 3.06e-23 | 99.4 |
| Msa0612970 | AT1G69570.1 | 41.270 | 126 | 63 | 3 | 52 | 167 | 134 | 258 | 3.85e-23 | 98.6 |
| Msa0612970 | AT1G64620.1 | 60.000 | 60 | 24 | 0 | 39 | 98 | 38 | 97 | 7.82e-23 | 97.1 |
| Msa0612970 | AT5G39660.3 | 58.462 | 65 | 27 | 0 | 39 | 103 | 127 | 191 | 8.24e-23 | 98.2 |
| Msa0612970 | AT5G39660.1 | 58.462 | 65 | 27 | 0 | 39 | 103 | 127 | 191 | 8.24e-23 | 98.2 |
| Msa0612970 | AT5G39660.2 | 58.462 | 65 | 27 | 0 | 39 | 103 | 127 | 191 | 8.24e-23 | 98.2 |
| Msa0612970 | AT1G29160.1 | 67.308 | 52 | 17 | 0 | 52 | 103 | 64 | 115 | 1.58e-22 | 92.4 |
| Msa0612970 | AT2G34140.1 | 64.815 | 54 | 19 | 0 | 50 | 103 | 58 | 111 | 1.60e-22 | 92.0 |
| Msa0612970 | AT5G62940.1 | 78.723 | 47 | 10 | 0 | 52 | 98 | 75 | 121 | 1.91e-22 | 96.3 |
| Msa0612970 | AT1G26790.1 | 66.667 | 54 | 18 | 0 | 50 | 103 | 111 | 164 | 2.57e-22 | 95.9 |
| Msa0612970 | AT4G21040.1 | 61.667 | 60 | 21 | 1 | 52 | 109 | 27 | 86 | 1.56e-21 | 91.3 |
| Msa0612970 | AT4G21080.1 | 44.706 | 85 | 47 | 0 | 52 | 136 | 27 | 111 | 2.76e-21 | 90.9 |
| Msa0612970 | AT4G21030.1 | 60.870 | 46 | 18 | 0 | 52 | 97 | 23 | 68 | 5.88e-13 | 66.6 |
Find 58 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCCAGTGCTTGGAATTAT+TGG | 0.224649 | 4_2:-61510740 | None:intergenic |
| TCTTCCAATGTTTATTACTA+TGG | 0.226715 | 4_2:+61510692 | Msa0612970:CDS |
| CTGATCTAAAGAATCCTTAT+TGG | 0.245243 | 4_2:+61510864 | Msa0612970:CDS |
| CTTATTAGAATCTCAAGATT+TGG | 0.267364 | 4_2:+61510085 | Msa0612970:CDS |
| AAAGTTCAAAGTATTGGAAT+TGG | 0.333779 | 4_2:+61510816 | Msa0612970:CDS |
| CAAATCTTGAGATTCTAATA+AGG | 0.337572 | 4_2:-61510084 | None:intergenic |
| TTGGGAAGACATTGATTCAT+TGG | 0.357990 | 4_2:+61510835 | Msa0612970:CDS |
| GTTGGTAATGGTAATGTTAA+TGG | 0.368454 | 4_2:+61510566 | Msa0612970:CDS |
| CTCATTAGATGATGAGGCTT+TGG | 0.369809 | 4_2:-61510113 | None:intergenic |
| TTATCAGAAAACAAAGGTTT+TGG | 0.373479 | 4_2:+61510542 | Msa0612970:CDS |
| TGATCTAAAGAATCCTTATT+GGG | 0.381365 | 4_2:+61510865 | Msa0612970:CDS |
| TTTCTAGCAGTGGACTTGAT+TGG | 0.385715 | 4_2:+61510054 | Msa0612970:CDS |
| AATGGCATGTTTACTAGTAC+TGG | 0.395485 | 4_2:+61510584 | Msa0612970:CDS |
| ATATCAGAATCATCCCAATA+AGG | 0.403157 | 4_2:-61510878 | None:intergenic |
| TTCTTTCTGCCACCACCAAC+AGG | 0.404107 | 4_2:-61510308 | None:intergenic |
| AAGTTCAAAGTATTGGAATT+GGG | 0.421160 | 4_2:+61510817 | Msa0612970:CDS |
| AAAAGAGCTTGAGGTTGAAA+AGG | 0.429717 | 4_2:-61510651 | None:intergenic |
| AGTAGTAGTTATGGTGGTGG+TGG | 0.454724 | 4_2:-61510369 | None:intergenic |
| CATGGGTTTGAGTTCTAAGC+AGG | 0.458863 | 4_2:+61510028 | None:intergenic |
| GCTATTCATATTTGTAATTG+AGG | 0.463164 | 4_2:-61510402 | None:intergenic |
| GGTGCAAGTAGTAGTAGTTA+TGG | 0.473348 | 4_2:-61510378 | None:intergenic |
| GCATGGAAAGTTCAAAGTAT+TGG | 0.482582 | 4_2:+61510810 | Msa0612970:CDS |
| AGAGCTTGATATAGAGATGA+AGG | 0.482587 | 4_2:-61510473 | None:intergenic |
| CCACCACTTGTTGATGCTGC+TGG | 0.497067 | 4_2:-61510788 | None:intergenic |
| GTGTTTGTTGAATCACATCT+TGG | 0.501929 | 4_2:-61510185 | None:intergenic |
| GTGTTAGTGCAGCCAGTGCT+TGG | 0.509724 | 4_2:-61510749 | None:intergenic |
| CTACGCAATGTTCCTGTTGG+TGG | 0.512399 | 4_2:+61510296 | Msa0612970:CDS |
| TGTAAAAGACACTGGACTAA+AGG | 0.514243 | 4_2:+61510266 | Msa0612970:CDS |
| AAAGAGCTTGAGGTTGAAAA+GGG | 0.516394 | 4_2:-61510650 | None:intergenic |
| TTCTGTTGTAGAAGCAAAGA+TGG | 0.517894 | 4_2:-61510506 | None:intergenic |
| CGCAATGTTCCTGTTGGTGG+TGG | 0.518071 | 4_2:+61510299 | Msa0612970:CDS |
| AAGCAGGTTATTTCTAGCAG+TGG | 0.536340 | 4_2:+61510044 | Msa0612970:CDS |
| GATGCTGCTGGTATCTCCCA+TGG | 0.542943 | 4_2:-61510776 | None:intergenic |
| GAACCAATAATTCCAAGCAC+TGG | 0.555313 | 4_2:+61510737 | Msa0612970:CDS |
| GGTTGAAAAGGGAAAGAGCA+TGG | 0.558342 | 4_2:-61510639 | None:intergenic |
| AGCATCAACAAGTGGTGGCA+TGG | 0.561290 | 4_2:+61510793 | Msa0612970:CDS |
| CTCTCCATAGTAATAAACAT+TGG | 0.562058 | 4_2:-61510696 | None:intergenic |
| ATTCAAACACCTCTTCAAGT+TGG | 0.568059 | 4_2:+61510434 | Msa0612970:CDS |
| GTGGTGGTGGAAGAAGTAAC+TGG | 0.571697 | 4_2:-61510356 | None:intergenic |
| AGTTGTGTCAAAAGAGCTTG+AGG | 0.572542 | 4_2:-61510660 | None:intergenic |
| GAAGAAGTAACTGGTGTGGT+TGG | 0.574360 | 4_2:-61510347 | None:intergenic |
| GTAGAGCTTGTAAAAGACAC+TGG | 0.584007 | 4_2:+61510258 | Msa0612970:CDS |
| GCTGCACTAACACAAAGCCA+TGG | 0.594454 | 4_2:+61510759 | Msa0612970:CDS |
| CAATGTTTATTACTATGGAG+AGG | 0.597791 | 4_2:+61510697 | Msa0612970:CDS |
| AAAAGACACTGGACTAAAGG+TGG | 0.598362 | 4_2:+61510269 | Msa0612970:CDS |
| ACTCTACGCAATGTTCCTGT+TGG | 0.603022 | 4_2:+61510293 | Msa0612970:CDS |
| ATTCATATTTGTAATTGAGG+TGG | 0.614882 | 4_2:-61510399 | None:intergenic |
| CTGCACTAACACAAAGCCAT+GGG | 0.628496 | 4_2:+61510760 | Msa0612970:CDS |
| AGTAGTAGTAGTTATGGTGG+TGG | 0.635889 | 4_2:-61510372 | None:intergenic |
| GTGGTGGCAGAAAGAATAAG+AGG | 0.637344 | 4_2:+61510315 | Msa0612970:CDS |
| CCAGCAGCATCAACAAGTGG+TGG | 0.642482 | 4_2:+61510788 | Msa0612970:CDS |
| GCAAGTAGTAGTAGTTATGG+TGG | 0.645803 | 4_2:-61510375 | None:intergenic |
| AATAACTTATCAGAAAACAA+AGG | 0.648214 | 4_2:+61510536 | Msa0612970:CDS |
| TACAAATATGAATAGCAACA+TGG | 0.667766 | 4_2:+61510409 | Msa0612970:CDS |
| CAAGCTCTACAGAAATGACG+AGG | 0.668993 | 4_2:-61510245 | None:intergenic |
| GGTGGAAGAAGTAACTGGTG+TGG | 0.685888 | 4_2:-61510351 | None:intergenic |
| ATACCAGCAGCATCAACAAG+TGG | 0.686074 | 4_2:+61510785 | Msa0612970:CDS |
| TGTTGTAGAAGCAAAGATGG+TGG | 0.693926 | 4_2:-61510503 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAACTTATCAGAAAACAA+AGG | + | chr4_2:61510536-61510555 | Msa0612970:CDS | 20.0% |
| !! | AATAACTTATCAGAAAACAA+AGG | + | chr4_2:61510536-61510555 | Msa0612970:CDS | 20.0% |
| ! | CAAATCTTGAGATTCTAATA+AGG | - | chr4_2:61510087-61510106 | None:intergenic | 25.0% |
| ! | CTTATTAGAATCTCAAGATT+TGG | + | chr4_2:61510085-61510104 | Msa0612970:CDS | 25.0% |
| ! | ATTCATATTTGTAATTGAGG+TGG | - | chr4_2:61510402-61510421 | None:intergenic | 25.0% |
| ! | GCTATTCATATTTGTAATTG+AGG | - | chr4_2:61510405-61510424 | None:intergenic | 25.0% |
| ! | TACAAATATGAATAGCAACA+TGG | + | chr4_2:61510409-61510428 | Msa0612970:CDS | 25.0% |
| !! | TTATCAGAAAACAAAGGTTT+TGG | + | chr4_2:61510542-61510561 | Msa0612970:CDS | 25.0% |
| ! | TCTTCCAATGTTTATTACTA+TGG | + | chr4_2:61510692-61510711 | Msa0612970:CDS | 25.0% |
| !!! | AAAGTTCAAAGTATTGGAAT+TGG | + | chr4_2:61510816-61510835 | Msa0612970:CDS | 25.0% |
| !!! | AAGTTCAAAGTATTGGAATT+GGG | + | chr4_2:61510817-61510836 | Msa0612970:CDS | 25.0% |
| ! | TGATCTAAAGAATCCTTATT+GGG | + | chr4_2:61510865-61510884 | Msa0612970:CDS | 25.0% |
| ! | CAAATCTTGAGATTCTAATA+AGG | - | chr4_2:61510087-61510106 | None:intergenic | 25.0% |
| ! | CTTATTAGAATCTCAAGATT+TGG | + | chr4_2:61510085-61510104 | Msa0612970:CDS | 25.0% |
| ! | ATTCATATTTGTAATTGAGG+TGG | - | chr4_2:61510402-61510421 | None:intergenic | 25.0% |
| ! | GCTATTCATATTTGTAATTG+AGG | - | chr4_2:61510405-61510424 | None:intergenic | 25.0% |
| ! | TACAAATATGAATAGCAACA+TGG | + | chr4_2:61510409-61510428 | Msa0612970:CDS | 25.0% |
| !! | TTATCAGAAAACAAAGGTTT+TGG | + | chr4_2:61510542-61510561 | Msa0612970:CDS | 25.0% |
| ! | TCTTCCAATGTTTATTACTA+TGG | + | chr4_2:61510692-61510711 | Msa0612970:CDS | 25.0% |
| !!! | AAAGTTCAAAGTATTGGAAT+TGG | + | chr4_2:61510816-61510835 | Msa0612970:CDS | 25.0% |
| !!! | AAGTTCAAAGTATTGGAATT+GGG | + | chr4_2:61510817-61510836 | Msa0612970:CDS | 25.0% |
| ! | TGATCTAAAGAATCCTTATT+GGG | + | chr4_2:61510865-61510884 | Msa0612970:CDS | 25.0% |
| ! | TGTTTTCTCATTAGATGATG+AGG | - | chr4_2:61510122-61510141 | None:intergenic | 30.0% |
| !! | GTTGGTAATGGTAATGTTAA+TGG | + | chr4_2:61510566-61510585 | Msa0612970:CDS | 30.0% |
| CTCTCCATAGTAATAAACAT+TGG | - | chr4_2:61510699-61510718 | None:intergenic | 30.0% | |
| CAATGTTTATTACTATGGAG+AGG | + | chr4_2:61510697-61510716 | Msa0612970:CDS | 30.0% | |
| CTGATCTAAAGAATCCTTAT+TGG | + | chr4_2:61510864-61510883 | Msa0612970:CDS | 30.0% | |
| ATATCAGAATCATCCCAATA+AGG | - | chr4_2:61510881-61510900 | None:intergenic | 30.0% | |
| ! | TGTTTTCTCATTAGATGATG+AGG | - | chr4_2:61510122-61510141 | None:intergenic | 30.0% |
| !! | GTTGGTAATGGTAATGTTAA+TGG | + | chr4_2:61510566-61510585 | Msa0612970:CDS | 30.0% |
| CTCTCCATAGTAATAAACAT+TGG | - | chr4_2:61510699-61510718 | None:intergenic | 30.0% | |
| CAATGTTTATTACTATGGAG+AGG | + | chr4_2:61510697-61510716 | Msa0612970:CDS | 30.0% | |
| CTGATCTAAAGAATCCTTAT+TGG | + | chr4_2:61510864-61510883 | Msa0612970:CDS | 30.0% | |
| ATATCAGAATCATCCCAATA+AGG | - | chr4_2:61510881-61510900 | None:intergenic | 30.0% | |
| ! | GTGTTTGTTGAATCACATCT+TGG | - | chr4_2:61510188-61510207 | None:intergenic | 35.0% |
| TGTAAAAGACACTGGACTAA+AGG | + | chr4_2:61510266-61510285 | Msa0612970:CDS | 35.0% | |
| ATTCAAACACCTCTTCAAGT+TGG | + | chr4_2:61510434-61510453 | Msa0612970:CDS | 35.0% | |
| ! | TTTTGATCACCAACTTGAAG+AGG | - | chr4_2:61510446-61510465 | None:intergenic | 35.0% |
| AGAGCTTGATATAGAGATGA+AGG | - | chr4_2:61510476-61510495 | None:intergenic | 35.0% | |
| TTCTGTTGTAGAAGCAAAGA+TGG | - | chr4_2:61510509-61510528 | None:intergenic | 35.0% | |
| !! | GAAAACAAAGGTTTTGGTGT+TGG | + | chr4_2:61510548-61510567 | Msa0612970:CDS | 35.0% |
| !!! | AAAGGTTTTGGTGTTGGTAA+TGG | + | chr4_2:61510554-61510573 | Msa0612970:CDS | 35.0% |
| AATGGCATGTTTACTAGTAC+TGG | + | chr4_2:61510584-61510603 | Msa0612970:CDS | 35.0% | |
| !! | TGGTTTGATTTTGATGAGGA+AGG | - | chr4_2:61510622-61510641 | None:intergenic | 35.0% |
| !! | AGCATGGTTTGATTTTGATG+AGG | - | chr4_2:61510626-61510645 | None:intergenic | 35.0% |
| AAAGAGCTTGAGGTTGAAAA+GGG | - | chr4_2:61510653-61510672 | None:intergenic | 35.0% | |
| AAAAGAGCTTGAGGTTGAAA+AGG | - | chr4_2:61510654-61510673 | None:intergenic | 35.0% | |
| GCATGGAAAGTTCAAAGTAT+TGG | + | chr4_2:61510810-61510829 | Msa0612970:CDS | 35.0% | |
| !! | TTGGGAAGACATTGATTCAT+TGG | + | chr4_2:61510835-61510854 | Msa0612970:CDS | 35.0% |
| ! | GTGTTTGTTGAATCACATCT+TGG | - | chr4_2:61510188-61510207 | None:intergenic | 35.0% |
| TGTAAAAGACACTGGACTAA+AGG | + | chr4_2:61510266-61510285 | Msa0612970:CDS | 35.0% | |
| ATTCAAACACCTCTTCAAGT+TGG | + | chr4_2:61510434-61510453 | Msa0612970:CDS | 35.0% | |
| ! | TTTTGATCACCAACTTGAAG+AGG | - | chr4_2:61510446-61510465 | None:intergenic | 35.0% |
| AGAGCTTGATATAGAGATGA+AGG | - | chr4_2:61510476-61510495 | None:intergenic | 35.0% | |
| TTCTGTTGTAGAAGCAAAGA+TGG | - | chr4_2:61510509-61510528 | None:intergenic | 35.0% | |
| !! | GAAAACAAAGGTTTTGGTGT+TGG | + | chr4_2:61510548-61510567 | Msa0612970:CDS | 35.0% |
| !!! | AAAGGTTTTGGTGTTGGTAA+TGG | + | chr4_2:61510554-61510573 | Msa0612970:CDS | 35.0% |
| AATGGCATGTTTACTAGTAC+TGG | + | chr4_2:61510584-61510603 | Msa0612970:CDS | 35.0% | |
| !! | TGGTTTGATTTTGATGAGGA+AGG | - | chr4_2:61510622-61510641 | None:intergenic | 35.0% |
| !! | AGCATGGTTTGATTTTGATG+AGG | - | chr4_2:61510626-61510645 | None:intergenic | 35.0% |
| AAAGAGCTTGAGGTTGAAAA+GGG | - | chr4_2:61510653-61510672 | None:intergenic | 35.0% | |
| AAAAGAGCTTGAGGTTGAAA+AGG | - | chr4_2:61510654-61510673 | None:intergenic | 35.0% | |
| GCATGGAAAGTTCAAAGTAT+TGG | + | chr4_2:61510810-61510829 | Msa0612970:CDS | 35.0% | |
| !! | TTGGGAAGACATTGATTCAT+TGG | + | chr4_2:61510835-61510854 | Msa0612970:CDS | 35.0% |
| !! | AAGCAGGTTATTTCTAGCAG+TGG | + | chr4_2:61510044-61510063 | Msa0612970:CDS | 40.0% |
| ! | TTTCTAGCAGTGGACTTGAT+TGG | + | chr4_2:61510054-61510073 | Msa0612970:CDS | 40.0% |
| CTCATTAGATGATGAGGCTT+TGG | - | chr4_2:61510116-61510135 | None:intergenic | 40.0% | |
| GTAGAGCTTGTAAAAGACAC+TGG | + | chr4_2:61510258-61510277 | Msa0612970:CDS | 40.0% | |
| AAAAGACACTGGACTAAAGG+TGG | + | chr4_2:61510269-61510288 | Msa0612970:CDS | 40.0% | |
| AGTAGTAGTAGTTATGGTGG+TGG | - | chr4_2:61510375-61510394 | None:intergenic | 40.0% | |
| GCAAGTAGTAGTAGTTATGG+TGG | - | chr4_2:61510378-61510397 | None:intergenic | 40.0% | |
| GGTGCAAGTAGTAGTAGTTA+TGG | - | chr4_2:61510381-61510400 | None:intergenic | 40.0% | |
| TGTTGTAGAAGCAAAGATGG+TGG | - | chr4_2:61510506-61510525 | None:intergenic | 40.0% | |
| AGTTGTGTCAAAAGAGCTTG+AGG | - | chr4_2:61510663-61510682 | None:intergenic | 40.0% | |
| GAACCAATAATTCCAAGCAC+TGG | + | chr4_2:61510737-61510756 | Msa0612970:CDS | 40.0% | |
| !! | AAGCAGGTTATTTCTAGCAG+TGG | + | chr4_2:61510044-61510063 | Msa0612970:CDS | 40.0% |
| ! | TTTCTAGCAGTGGACTTGAT+TGG | + | chr4_2:61510054-61510073 | Msa0612970:CDS | 40.0% |
| CTCATTAGATGATGAGGCTT+TGG | - | chr4_2:61510116-61510135 | None:intergenic | 40.0% | |
| GTAGAGCTTGTAAAAGACAC+TGG | + | chr4_2:61510258-61510277 | Msa0612970:CDS | 40.0% | |
| AAAAGACACTGGACTAAAGG+TGG | + | chr4_2:61510269-61510288 | Msa0612970:CDS | 40.0% | |
| AGTAGTAGTAGTTATGGTGG+TGG | - | chr4_2:61510375-61510394 | None:intergenic | 40.0% | |
| GCAAGTAGTAGTAGTTATGG+TGG | - | chr4_2:61510378-61510397 | None:intergenic | 40.0% | |
| GGTGCAAGTAGTAGTAGTTA+TGG | - | chr4_2:61510381-61510400 | None:intergenic | 40.0% | |
| TGTTGTAGAAGCAAAGATGG+TGG | - | chr4_2:61510506-61510525 | None:intergenic | 40.0% | |
| AGTTGTGTCAAAAGAGCTTG+AGG | - | chr4_2:61510663-61510682 | None:intergenic | 40.0% | |
| GAACCAATAATTCCAAGCAC+TGG | + | chr4_2:61510737-61510756 | Msa0612970:CDS | 40.0% | |
| CAAGCTCTACAGAAATGACG+AGG | - | chr4_2:61510248-61510267 | None:intergenic | 45.0% | |
| ACTCTACGCAATGTTCCTGT+TGG | + | chr4_2:61510293-61510312 | Msa0612970:CDS | 45.0% | |
| GTGGTGGCAGAAAGAATAAG+AGG | + | chr4_2:61510315-61510334 | Msa0612970:CDS | 45.0% | |
| GAAGAAGTAACTGGTGTGGT+TGG | - | chr4_2:61510350-61510369 | None:intergenic | 45.0% | |
| !! | AGTAGTAGTTATGGTGGTGG+TGG | - | chr4_2:61510372-61510391 | None:intergenic | 45.0% |
| GGTTGAAAAGGGAAAGAGCA+TGG | - | chr4_2:61510642-61510661 | None:intergenic | 45.0% | |
| CAGCCAGTGCTTGGAATTAT+TGG | - | chr4_2:61510743-61510762 | None:intergenic | 45.0% | |
| CTGCACTAACACAAAGCCAT+GGG | + | chr4_2:61510760-61510779 | Msa0612970:CDS | 45.0% | |
| ATACCAGCAGCATCAACAAG+TGG | + | chr4_2:61510785-61510804 | Msa0612970:CDS | 45.0% | |
| CAAGCTCTACAGAAATGACG+AGG | - | chr4_2:61510248-61510267 | None:intergenic | 45.0% | |
| ACTCTACGCAATGTTCCTGT+TGG | + | chr4_2:61510293-61510312 | Msa0612970:CDS | 45.0% | |
| GTGGTGGCAGAAAGAATAAG+AGG | + | chr4_2:61510315-61510334 | Msa0612970:CDS | 45.0% | |
| GAAGAAGTAACTGGTGTGGT+TGG | - | chr4_2:61510350-61510369 | None:intergenic | 45.0% | |
| !! | AGTAGTAGTTATGGTGGTGG+TGG | - | chr4_2:61510372-61510391 | None:intergenic | 45.0% |
| GGTTGAAAAGGGAAAGAGCA+TGG | - | chr4_2:61510642-61510661 | None:intergenic | 45.0% | |
| CAGCCAGTGCTTGGAATTAT+TGG | - | chr4_2:61510743-61510762 | None:intergenic | 45.0% | |
| CTGCACTAACACAAAGCCAT+GGG | + | chr4_2:61510760-61510779 | Msa0612970:CDS | 45.0% | |
| ATACCAGCAGCATCAACAAG+TGG | + | chr4_2:61510785-61510804 | Msa0612970:CDS | 45.0% | |
| CTACGCAATGTTCCTGTTGG+TGG | + | chr4_2:61510296-61510315 | Msa0612970:CDS | 50.0% | |
| TTCTTTCTGCCACCACCAAC+AGG | - | chr4_2:61510311-61510330 | None:intergenic | 50.0% | |
| GGTGGAAGAAGTAACTGGTG+TGG | - | chr4_2:61510354-61510373 | None:intergenic | 50.0% | |
| !! | GTGGTGGTGGAAGAAGTAAC+TGG | - | chr4_2:61510359-61510378 | None:intergenic | 50.0% |
| GCTGCACTAACACAAAGCCA+TGG | + | chr4_2:61510759-61510778 | Msa0612970:CDS | 50.0% | |
| AGCATCAACAAGTGGTGGCA+TGG | + | chr4_2:61510793-61510812 | Msa0612970:CDS | 50.0% | |
| CTACGCAATGTTCCTGTTGG+TGG | + | chr4_2:61510296-61510315 | Msa0612970:CDS | 50.0% | |
| TTCTTTCTGCCACCACCAAC+AGG | - | chr4_2:61510311-61510330 | None:intergenic | 50.0% | |
| GGTGGAAGAAGTAACTGGTG+TGG | - | chr4_2:61510354-61510373 | None:intergenic | 50.0% | |
| !! | GTGGTGGTGGAAGAAGTAAC+TGG | - | chr4_2:61510359-61510378 | None:intergenic | 50.0% |
| GCTGCACTAACACAAAGCCA+TGG | + | chr4_2:61510759-61510778 | Msa0612970:CDS | 50.0% | |
| AGCATCAACAAGTGGTGGCA+TGG | + | chr4_2:61510793-61510812 | Msa0612970:CDS | 50.0% | |
| ! | CGCAATGTTCCTGTTGGTGG+TGG | + | chr4_2:61510299-61510318 | Msa0612970:CDS | 55.0% |
| GTGTTAGTGCAGCCAGTGCT+TGG | - | chr4_2:61510752-61510771 | None:intergenic | 55.0% | |
| GATGCTGCTGGTATCTCCCA+TGG | - | chr4_2:61510779-61510798 | None:intergenic | 55.0% | |
| ! | CCACCACTTGTTGATGCTGC+TGG | - | chr4_2:61510791-61510810 | None:intergenic | 55.0% |
| CCAGCAGCATCAACAAGTGG+TGG | + | chr4_2:61510788-61510807 | Msa0612970:CDS | 55.0% | |
| ! | CGCAATGTTCCTGTTGGTGG+TGG | + | chr4_2:61510299-61510318 | Msa0612970:CDS | 55.0% |
| GTGTTAGTGCAGCCAGTGCT+TGG | - | chr4_2:61510752-61510771 | None:intergenic | 55.0% | |
| GATGCTGCTGGTATCTCCCA+TGG | - | chr4_2:61510779-61510798 | None:intergenic | 55.0% | |
| ! | CCACCACTTGTTGATGCTGC+TGG | - | chr4_2:61510791-61510810 | None:intergenic | 55.0% |
| CCAGCAGCATCAACAAGTGG+TGG | + | chr4_2:61510788-61510807 | Msa0612970:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 61510029 | 61510910 | 61510029 | ID=Msa0612970;Name=Msa0612970 |
| chr4_2 | mRNA | 61510029 | 61510910 | 61510029 | ID=Msa0612970-mRNA-1;Parent=Msa0612970;Name=Msa0612970-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|293 |
| chr4_2 | exon | 61510029 | 61510910 | 61510029 | ID=Msa0612970-mRNA-1:exon:16798;Parent=Msa0612970-mRNA-1 |
| chr4_2 | CDS | 61510029 | 61510910 | 61510029 | ID=Msa0612970-mRNA-1:cds;Parent=Msa0612970-mRNA-1 |
| Gene Sequence |
| Protein sequence |
