Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | XP_013457087.1 | 95.973 | 298 | 7 | 2 | 1 | 293 | 1 | 298 | 0.0 | 587 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | sp|Q9FZA4|DOF14_ARATH | 81.356 | 59 | 10 | 1 | 50 | 107 | 27 | 85 | 1.65e-28 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | A0A072UPW6 | 95.973 | 298 | 7 | 2 | 1 | 293 | 1 | 298 | 0.0 | 587 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | MtrunA17_Chr4g0047761 | 95.804 | 286 | 7 | 2 | 13 | 293 | 35 | 320 | 0.0 | 564 |
| Msa0612970 | MtrunA17_Chr2g0282051 | 58.689 | 305 | 83 | 14 | 1 | 293 | 1 | 274 | 8.30e-97 | 286 |
| Msa0612970 | MtrunA17_Chr2g0328021 | 41.989 | 181 | 82 | 6 | 50 | 227 | 62 | 222 | 3.29e-31 | 117 |
| Msa0612970 | MtrunA17_Chr4g0047001 | 54.472 | 123 | 45 | 3 | 17 | 132 | 16 | 134 | 1.38e-30 | 118 |
| Msa0612970 | MtrunA17_Chr3g0127201 | 67.105 | 76 | 20 | 2 | 29 | 103 | 10 | 81 | 3.72e-29 | 112 |
| Msa0612970 | MtrunA17_Chr8g0374251 | 59.259 | 81 | 32 | 1 | 48 | 127 | 66 | 146 | 8.01e-29 | 112 |
| Msa0612970 | MtrunA17_Chr8g0374111 | 60.256 | 78 | 30 | 1 | 46 | 122 | 25 | 102 | 9.00e-29 | 110 |
| Msa0612970 | MtrunA17_Chr2g0326001 | 31.923 | 260 | 144 | 10 | 36 | 269 | 25 | 277 | 1.36e-27 | 108 |
| Msa0612970 | MtrunA17_Chr8g0346551 | 55.422 | 83 | 36 | 1 | 37 | 118 | 11 | 93 | 2.65e-27 | 107 |
| Msa0612970 | MtrunA17_Chr3g0126201 | 55.556 | 99 | 42 | 2 | 9 | 105 | 9 | 107 | 1.32e-26 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0612970 | AT1G28310.1 | 81.356 | 59 | 10 | 1 | 50 | 107 | 27 | 85 | 1.68e-29 | 114 |
| Msa0612970 | AT3G55370.1 | 41.081 | 185 | 90 | 6 | 50 | 227 | 76 | 248 | 2.21e-29 | 114 |
| Msa0612970 | AT1G28310.2 | 81.356 | 59 | 10 | 1 | 50 | 107 | 51 | 109 | 2.54e-29 | 114 |
| Msa0612970 | AT3G55370.2 | 41.081 | 185 | 90 | 6 | 50 | 227 | 76 | 248 | 2.61e-29 | 114 |
| Msa0612970 | AT3G55370.3 | 41.081 | 185 | 90 | 6 | 50 | 227 | 121 | 293 | 3.95e-29 | 114 |
| Msa0612970 | AT5G60850.1 | 69.863 | 73 | 21 | 1 | 50 | 121 | 51 | 123 | 1.85e-28 | 111 |
| Msa0612970 | AT1G51700.1 | 60.274 | 73 | 29 | 0 | 38 | 110 | 21 | 93 | 6.77e-28 | 107 |
| Msa0612970 | AT5G66940.1 | 51.546 | 97 | 45 | 2 | 23 | 119 | 6 | 100 | 2.21e-27 | 106 |
| Msa0612970 | AT5G60200.1 | 80.000 | 55 | 10 | 1 | 50 | 103 | 55 | 109 | 4.64e-27 | 106 |
| Msa0612970 | AT5G65590.1 | 53.933 | 89 | 34 | 2 | 28 | 109 | 13 | 101 | 4.72e-27 | 107 |
Find 58 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGACACTGGACTAAAGG+TGG | 0.598362 | 4_2:+61510269 | Msa0612970:CDS |
| AAAAGAGCTTGAGGTTGAAA+AGG | 0.429717 | 4_2:-61510651 | None:intergenic |
| AAAGAGCTTGAGGTTGAAAA+GGG | 0.516394 | 4_2:-61510650 | None:intergenic |
| AAAGTTCAAAGTATTGGAAT+TGG | 0.333779 | 4_2:+61510816 | Msa0612970:CDS |
| AAGCAGGTTATTTCTAGCAG+TGG | 0.536340 | 4_2:+61510044 | Msa0612970:CDS |
| AAGTTCAAAGTATTGGAATT+GGG | 0.421160 | 4_2:+61510817 | Msa0612970:CDS |
| AATAACTTATCAGAAAACAA+AGG | 0.648214 | 4_2:+61510536 | Msa0612970:CDS |
| AATGGCATGTTTACTAGTAC+TGG | 0.395485 | 4_2:+61510584 | Msa0612970:CDS |
| ACTCTACGCAATGTTCCTGT+TGG | 0.603022 | 4_2:+61510293 | Msa0612970:CDS |
| AGAGCTTGATATAGAGATGA+AGG | 0.482587 | 4_2:-61510473 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCTCCATAGTAATAAACAT+TGG | - | chr4_2:61510699-61510718 | None:intergenic | 30.0% | |
| CAATGTTTATTACTATGGAG+AGG | + | chr4_2:61510697-61510716 | Msa0612970:CDS | 30.0% | |
| CTGATCTAAAGAATCCTTAT+TGG | + | chr4_2:61510864-61510883 | Msa0612970:CDS | 30.0% | |
| ATATCAGAATCATCCCAATA+AGG | - | chr4_2:61510881-61510900 | None:intergenic | 30.0% | |
| CTCTCCATAGTAATAAACAT+TGG | - | chr4_2:61510699-61510718 | None:intergenic | 30.0% | |
| CAATGTTTATTACTATGGAG+AGG | + | chr4_2:61510697-61510716 | Msa0612970:CDS | 30.0% | |
| CTGATCTAAAGAATCCTTAT+TGG | + | chr4_2:61510864-61510883 | Msa0612970:CDS | 30.0% | |
| ATATCAGAATCATCCCAATA+AGG | - | chr4_2:61510881-61510900 | None:intergenic | 30.0% | |
| TGTAAAAGACACTGGACTAA+AGG | + | chr4_2:61510266-61510285 | Msa0612970:CDS | 35.0% | |
| ATTCAAACACCTCTTCAAGT+TGG | + | chr4_2:61510434-61510453 | Msa0612970:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 61510029 | 61510910 | 61510029 | ID=Msa0612970;Name=Msa0612970 |
| chr4_2 | mRNA | 61510029 | 61510910 | 61510029 | ID=Msa0612970-mRNA-1;Parent=Msa0612970;Name=Msa0612970-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|293 |
| chr4_2 | exon | 61510029 | 61510910 | 61510029 | ID=Msa0612970-mRNA-1:exon:16798;Parent=Msa0612970-mRNA-1 |
| chr4_2 | CDS | 61510029 | 61510910 | 61510029 | ID=Msa0612970-mRNA-1:cds;Parent=Msa0612970-mRNA-1 |
| Gene Sequence |
>Msa0612970 ATGGGTTTGAGTTCTAAGCAGGTTATTTCTAGCAGTGGACTTGATTGGAAACAAACCTTATTAGAATCTCAAGATTTGGAACTTCCAAAGCCTCATCATCTAATGAGAAAACAACAACAGCAACAAAGTCAGCAACAAGCAGAACATTTGAACTGTCCAAGATGTGATTCAACAAACACAAAGTTTTGTTACTACAACAATTACAACAAGTCTCAACCTCGTCATTTCTGTAGAGCTTGTAAAAGACACTGGACTAAAGGTGGAACTCTACGCAATGTTCCTGTTGGTGGTGGCAGAAAGAATAAGAGGATCAAGAAACCAACCACACCAGTTACTTCTTCCACCACCACCATAACTACTACTACTTGCACCACCTCAATTACAAATATGAATAGCAACATGGCAATTCAAACACCTCTTCAAGTTGGTGATCAAAAAAGCATTCCTTCATCTCTATATCAAGCTCTCATTCATCCACCATCTTTGCTTCTACAACAGAATATGATGAATAACTTATCAGAAAACAAAGGTTTTGGTGTTGGTAATGGTAATGTTAATGGCATGTTTACTAGTACTGGTTCAACTTTGCACCTTCCTCATCAAAATCAAACCATGCTCTTTCCCTTTTCAACCTCAAGCTCTTTTGACACAACTACTTGTTCATCTTCCAATGTTTATTACTATGGAGAGGAGTTCAAAACAATAGAAGAACCAATAATTCCAAGCACTGGCTGCACTAACACAAAGCCATGGGAGATACCAGCAGCATCAACAAGTGGTGGCATGGAAAGTTCAAAGTATTGGAATTGGGAAGACATTGATTCATTGGTCTCAACTGATCTAAAGAATCCTTATTGGGATGATTCTGATATCAAACCTTGA |
| Protein sequence |
>Msa0612970 MGLSSKQVISSSGLDWKQTLLESQDLELPKPHHLMRKQQQQQSQQQAEHLNCPRCDSTNTKFCYYNNYNKSQPRHFCRACKRHWTKGGTLRNVPVGGGRKNKRIKKPTTPVTSSTTTITTTTCTTSITNMNSNMAIQTPLQVGDQKSIPSSLYQALIHPPSLLLQQNMMNNLSENKGFGVGNGNVNGMFTSTGSTLHLPHQNQTMLFPFSTSSSFDTTTCSSSNVYYYGEEFKTIEEPIIPSTGCTNTKPWEIPAASTSGGMESSKYWNWEDIDSLVSTDLKNPYWDDSDIKP* |
