AlfalfaGEDB | Msa0615150

M. sativa cultivar zhongmu-4 / Msa0615150

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615150	XP_013457284.1	94.805	539	28	0	1	539	1	539	0.0	1071

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615150	sp\|Q0WVM4\|Y2239_ARATH	23.875	511	278	17	124	539	100	594	3.19e-28	122

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615150	MtrunA17_Chr4g0051331	94.805	539	28	0	1	539	1	539	0.0	1071
Msa0615150	MtrunA17_Chr4g0013491	30.818	318	188	9	122	414	294	604	6.49e-32	130
Msa0615150	MtrunA17_Chr1g0201241	23.780	492	291	18	94	507	513	998	2.85e-29	123
Msa0615150	MtrunA17_Chr8g0363501	29.063	320	194	10	122	414	74	387	8.11e-29	120
Msa0615150	MtrunA17_Chr8g0363221	24.424	434	271	11	123	503	414	843	2.67e-28	119
Msa0615150	MtrunA17_Chr8g0362981	24.236	458	282	13	122	521	294	744	5.32e-28	119
Msa0615150	MtrunA17_Chr8g0362871	27.445	317	201	8	123	414	329	641	8.12e-28	118
Msa0615150	MtrunA17_Chr8g0363111	28.571	336	203	9	122	428	538	865	9.34e-28	118
Msa0615150	MtrunA17_Chr8g0361231	28.797	316	193	10	123	414	636	943	2.06e-27	117
Msa0615150	MtrunA17_Chr8g0363241	28.483	323	192	10	122	414	296	609	3.33e-27	116

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615150	AT4G20790.1	51.744	516	238	7	6	514	3	514	0.0	530
Msa0615150	AT2G23950.1	23.875	511	278	17	124	539	100	594	3.24e-29	122
Msa0615150	AT4G30520.1	23.152	514	281	16	123	539	102	598	4.81e-26	112
Msa0615150	AT2G23950.2	27.143	350	192	11	124	414	100	445	1.21e-25	110
Msa0615150	AT1G34110.2	22.517	453	276	14	126	507	601	1049	5.23e-25	110
Msa0615150	AT1G34110.1	22.517	453	276	14	126	507	601	1049	5.58e-25	110
Msa0615150	AT3G55550.1	24.585	301	190	6	257	521	332	631	2.48e-24	107
Msa0615150	AT3G59110.1	25.503	298	183	8	247	507	166	461	3.53e-24	106
Msa0615150	AT2G20300.1	27.083	288	173	8	258	511	336	620	3.99e-24	107
Msa0615150	AT5G45780.2	22.292	480	292	16	124	529	86	558	5.19e-24	106

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Find 87 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGTAGTGTTGAAATC+TGG	0.416076	4_2:+64535677	None:intergenic
AAACCACAAGAATCTTGCTA+AGG	0.548672	4_2:-64534832	Msa0615150:CDS
AAACCTTAGCAAGATTCTTG+TGG	0.561241	4_2:+64534829	None:intergenic
AAAGAGATGCAATGTTTGGT+CGG	0.549488	4_2:+64535495	None:intergenic
AAATAAAGAACAACCAAGCT+TGG	0.541589	4_2:-64534011	Msa0615150:CDS
AACAATGTTGATAAAATTAT+AGG	0.322342	4_2:+64535761	None:intergenic
AACTTTGCACCAAGTTACAC+TGG	0.544145	4_2:-64534068	Msa0615150:CDS
AAGAAAGGTTAAGAAAAGTG+AGG	0.574505	4_2:+64535285	None:intergenic
AAGTAAAGAGATGCAATGTT+TGG	0.479785	4_2:+64535491	None:intergenic
AAGTGAAAATTGAGATGTAT+TGG	0.458478	4_2:-64534917	Msa0615150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTAGATTCATTGCAAAGA+TGG	+	chr4_2:64534134-64534153	None:intergenic	30.0%
GAAATGATTCAACACAATTG+GGG	+	chr4_2:64534158-64534177	None:intergenic	30.0%
GGAAATGATTCAACACAATT+GGG	+	chr4_2:64534159-64534178	None:intergenic	30.0%
AAGTAAAGAGATGCAATGTT+TGG	+	chr4_2:64534204-64534223	None:intergenic	30.0%
TTTCTCCAACAATTCCATAA+AGG	-	chr4_2:64534275-64534294	Msa0615150:intron	30.0%
TTCTCCAACAATTCCATAAA+GGG	-	chr4_2:64534276-64534295	Msa0615150:intron	30.0%
GTGCAATAAAGAACATCATT+TGG	+	chr4_2:64534309-64534328	None:intergenic	30.0%
AGTGAAGAGAAACTTTGAAT+TGG	+	chr4_2:64534381-64534400	None:intergenic	30.0%
AAGAAAGGTTAAGAAAAGTG+AGG	+	chr4_2:64534410-64534429	None:intergenic	30.0%
CTGAGAAACAGTTATAAGAA+AGG	+	chr4_2:64534425-64534444	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	64533892	64535822	64533892	ID=Msa0615150;Name=Msa0615150
chr4_2	mRNA	64533892	64535822	64533892	ID=Msa0615150-mRNA-1;Parent=Msa0615150;Name=Msa0615150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|0\|0\|1\|1\|1\|2\|0\|539
chr4_2	exon	64534562	64535822	64534562	ID=Msa0615150-mRNA-1:exon:18100;Parent=Msa0615150-mRNA-1
chr4_2	exon	64533892	64534250	64533892	ID=Msa0615150-mRNA-1:exon:18099;Parent=Msa0615150-mRNA-1
chr4_2	CDS	64534562	64535822	64534562	ID=Msa0615150-mRNA-1:cds;Parent=Msa0615150-mRNA-1
chr4_2	CDS	64533892	64534250	64533892	ID=Msa0615150-mRNA-1:cds;Parent=Msa0615150-mRNA-1

Gene Sequence

>Msa0615150

ATGGCTACCACCATTTCATCAACTTGCTTCCTCTTTGTTCCTATAATTTTATCAACATTGTTATGTCCCAAAACAACATTATGTGCAACTGTCCTAGAAGACCTCAAAAACTTACACAAACCACCAGATTTCAACACTACAATTTTCAACAACTGTCTCAAAAACCCATCTCTCAGATACTGCACTTCCTCTCCAATGGTGGACCTTGATGAAATATTCAAACACACTATTGTTGCTAGCCATCTTTGCAATGAATCTAACAACCCCAATTGTGTTGAATCATTTCCCAAAATAGATCTTCGAAACCGACCAAACATTGCATCTCTTTACTTGTCTTTCACGTTCTTTTGGAAATATTGTCCTTTGAGTATTCAATCCATTGATTTCTCCAACAATTCCATAAAGGGTGGTTTTCCAAATGATGTTCTTTATTGCACACAAATTCAGTCTCTTGATTTAAGTATCAATGAATTTTCTGGTGAAATTCCAATTCAAAGTTTCTCTTCACTAACTAACCTCACTTTTCTTAACCTTTCTTATAACTGTTTCTCAGAGAGTGAAATTTCTGATTCGCAATTCTTCAAAAAGTTTAATGCTTCTAGTTTTCTTCATTCTGGTGCTCTCATTGATCACAAAAAGTTTACTATAAAAGCTATCATTTTATTGGTTGGTTTTCCTATTCTTGTCATTTTGATGGTAATTTTCATGGGATGGCTTTGTTTTCAAAGACCTGATTTTCTGCCAAGAATACTTCAAAGTAGTAGAGGATATACATTTACACCAGCAATTCTAAAGGCTGCAACTTGTGGTTTTTCGAACAAAAATTTGGTTGGTAAGAGTGATATTGTCCATATCTACAAAGGGGTGTTAAGAGATGGAACTGAAGTGAAAATTGAGATGTATTGGGATGATATATCAAGGGATAGTTATCATAAATTTGTTGAGGAATGCAAGATTCTGTCTGAGTTAAACCACAAGAATCTTGCTAAGGTTTTAGGATGGTGTAAAGGTAGAAAATTCAGGGCTATTATAACAGAATGGACAAACGACGTAAATGTTGAAATGTGGCTATCAGGGGCAAGTCCATCATGGAATCATAGAGTGAAAGTTTTGATGGGAGTTGTTGAATGCATGCTATATCTTCATGAGGAGTGGCCTGAAGTTGATTACGATCTCAAGACAAGTAGTGTTTTGCTATCTGATACACTAGAACCATTGATTTCAAGGTTTCAAGTTGGAGAAAGAAACAACACTCGAAAAAAGGTGTGTAAGTTTGGAGTATTTCTGCTAGAGATGATACTAAACAAGAGCGTTCAAGAGGACTTTGATGGAGATGATGCCGGTTTTGTTAGCTACATGAGAAGCCTTCATCTTGCAGACTTGCAGCAGATGATTGATGAGAGATTAAATGTTACTGAAACAACTTTGCACCAAGTTACACTGGCTTTATCCTTGGGATTAATGTGCATAGACCAATCAAATAAAGAACAACCAAGCTTGGCACAAATATTTAATATTGTATCAAAGGCATACAAGGCTAACCTTGTTTTGCCATCTGCAAATCATAAAACATTTCATGGAGATAAAGTTAAGGGACACAAGCATGTTCAGTTCAGATAA

Protein sequence

>Msa0615150

MATTISSTCFLFVPIILSTLLCPKTTLCATVLEDLKNLHKPPDFNTTIFNNCLKNPSLRYCTSSPMVDLDEIFKHTIVASHLCNESNNPNCVESFPKIDLRNRPNIASLYLSFTFFWKYCPLSIQSIDFSNNSIKGGFPNDVLYCTQIQSLDLSINEFSGEIPIQSFSSLTNLTFLNLSYNCFSESEISDSQFFKKFNASSFLHSGALIDHKKFTIKAIILLVGFPILVILMVIFMGWLCFQRPDFLPRILQSSRGYTFTPAILKAATCGFSNKNLVGKSDIVHIYKGVLRDGTEVKIEMYWDDISRDSYHKFVEECKILSELNHKNLAKVLGWCKGRKFRAIITEWTNDVNVEMWLSGASPSWNHRVKVLMGVVECMLYLHEEWPEVDYDLKTSSVLLSDTLEPLISRFQVGERNNTRKKVCKFGVFLLEMILNKSVQEDFDGDDAGFVSYMRSLHLADLQQMIDERLNVTETTLHQVTLALSLGLMCIDQSNKEQPSLAQIFNIVSKAYKANLVLPSANHKTFHGDKVKGHKHVQFR*