AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0617520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0617520 MtrunA17_Chr8g0377401 95.257 253 12 0 1 253 1 253 1.02e-158 448
Msa0617520 MtrunA17_Chr8g0377421 60.116 346 76 2 1 330 1 300 1.75e-136 394
Msa0617520 MtrunA17_Chr3g0122411 50.552 453 187 12 1 435 1 434 4.75e-115 343
Msa0617520 MtrunA17_Chr8g0377391 77.976 168 21 1 259 410 1 168 4.27e-81 247
Msa0617520 MtrunA17_Chr8g0377431 55.844 231 59 5 1 215 1 204 1.01e-57 188
Msa0617520 MtrunA17_Chr8g0360361 63.158 76 28 0 159 234 62 137 3.64e-29 112
Msa0617520 MtrunA17_Chr7g0258471 65.789 76 26 0 159 234 139 214 3.62e-28 115
Msa0617520 MtrunA17_Chr8g0377411 63.043 92 32 1 331 422 1 90 1.18e-27 105
Msa0617520 MtrunA17_Chr4g0046991 58.140 86 34 1 150 235 63 146 3.07e-27 106
Msa0617520 MtrunA17_Chr8g0334671 44.521 146 70 4 94 234 120 259 3.58e-27 110
Msa0617520 MtrunA17_Chr7g0231911 45.038 131 52 2 116 234 99 221 8.08e-26 107
Msa0617520 MtrunA17_Chr5g0420051 58.667 75 31 0 160 234 179 253 7.58e-25 106
Msa0617520 MtrunA17_Chr4g0061651 39.490 157 71 5 99 234 42 195 2.67e-24 104
Msa0617520 MtrunA17_Chr2g0282521 57.333 75 32 0 160 234 62 136 6.28e-24 97.1
Msa0617520 MtrunA17_Chr7g0224821 51.765 85 41 0 150 234 65 149 2.62e-23 100
Msa0617520 MtrunA17_Chr3g0132091 45.872 109 57 1 162 270 73 179 7.40e-23 99.0
Msa0617520 MtrunA17_Chr2g0308321 41.406 128 59 3 122 234 117 243 1.01e-22 100
Msa0617520 MtrunA17_Chr1g0175031 48.077 104 36 2 123 208 17 120 1.23e-21 90.5
Msa0617520 MtrunA17_Chr1g0162871 45.872 109 51 2 127 234 75 176 1.66e-21 97.8
Msa0617520 MtrunA17_Chr2g0302791 46.218 119 56 2 127 241 104 218 2.08e-21 97.4
Msa0617520 MtrunA17_Chr8g0386271 44.737 114 60 1 162 275 99 209 2.16e-21 95.5
Msa0617520 MtrunA17_Chr1g0194121 56.757 74 32 0 161 234 146 219 8.68e-21 95.5
Msa0617520 MtrunA17_Chr2g0286661 34.483 174 97 5 162 329 103 265 2.35e-20 94.0
Msa0617520 MtrunA17_Chr7g0270541 38.125 160 87 4 127 286 113 260 2.59e-20 94.0
Msa0617520 MtrunA17_Chr2g0317041 50.769 65 31 1 144 208 82 145 1.64e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0617520 AT1G27360.2 42.745 255 117 8 1 234 1 247 2.06e-38 143
Msa0617520 AT1G27360.1 42.745 255 117 8 1 234 1 247 2.06e-38 143
Msa0617520 AT1G27360.3 42.745 255 117 8 1 234 1 247 2.06e-38 143
Msa0617520 AT1G27360.4 42.745 255 117 8 1 234 1 247 2.06e-38 143
Msa0617520 AT5G43270.3 34.483 435 194 21 1 393 1 386 2.74e-38 144
Msa0617520 AT5G43270.1 34.483 435 194 21 1 393 1 386 2.74e-38 144
Msa0617520 AT5G43270.2 34.483 435 194 21 1 393 1 386 2.74e-38 144
Msa0617520 AT1G27370.6 41.633 245 114 7 12 234 11 248 7.77e-37 138
Msa0617520 AT1G27370.1 41.633 245 114 7 12 234 11 248 2.25e-36 138
Msa0617520 AT1G27370.5 41.633 245 114 7 12 234 11 248 2.25e-36 138
Msa0617520 AT1G27370.3 41.633 245 114 7 12 234 11 248 2.25e-36 138
Msa0617520 AT1G27370.2 41.633 245 114 7 12 234 11 248 2.25e-36 138
Msa0617520 AT1G27370.4 41.633 245 114 7 12 234 11 248 2.25e-36 138
Msa0617520 AT1G27370.7 41.633 245 114 7 12 234 11 248 2.25e-36 138
Msa0617520 AT2G42200.1 45.517 145 56 4 160 304 72 193 9.82e-29 116
Msa0617520 AT3G57920.1 49.541 109 48 2 129 234 27 131 1.90e-27 112
Msa0617520 AT1G02065.1 50.000 114 51 1 127 234 147 260 4.23e-27 111
Msa0617520 AT1G69170.1 62.069 87 30 2 150 234 111 196 6.27e-27 112
Msa0617520 AT2G33810.1 58.667 75 31 0 161 235 53 127 8.06e-25 99.4
Msa0617520 AT3G15270.1 56.757 74 32 0 161 234 62 135 2.97e-23 96.7
Msa0617520 AT1G53160.2 58.108 74 31 0 161 234 53 126 2.60e-22 94.0
Msa0617520 AT1G53160.1 58.108 74 31 0 161 234 53 126 2.60e-22 94.0
Msa0617520 AT1G53160.3 58.108 74 31 0 161 234 53 126 2.69e-22 93.6
Msa0617520 AT5G50570.1 54.667 75 34 0 160 234 99 173 3.19e-22 97.8
Msa0617520 AT5G50570.2 54.667 75 34 0 160 234 99 173 3.19e-22 97.8
Msa0617520 AT5G50670.1 54.667 75 34 0 160 234 99 173 3.19e-22 97.8
Msa0617520 AT5G50670.2 54.667 75 34 0 160 234 99 173 3.19e-22 97.8
Msa0617520 AT3G60030.1 57.534 73 31 0 162 234 127 199 2.50e-21 97.4
Msa0617520 AT2G47070.1 48.958 96 42 1 139 234 90 178 1.00e-20 95.5
Msa0617520 AT1G20980.1 56.000 75 33 0 160 234 118 192 4.03e-20 94.0
Msa0617520 AT1G76580.2 56.000 75 33 0 160 234 112 186 1.78e-19 91.7
Msa0617520 AT1G76580.3 56.000 75 33 0 160 234 112 186 1.96e-19 91.7
Msa0617520 AT1G76580.1 56.000 75 33 0 160 234 112 186 3.54e-19 90.9
Msa0617520 AT1G02065.2 47.191 89 41 1 127 209 147 235 2.07e-17 82.0
Msa0617520 AT5G18830.1 46.575 73 39 0 162 234 138 210 6.30e-16 80.9
Msa0617520 AT5G18830.3 46.575 73 39 0 162 234 138 210 6.31e-16 80.9
Msa0617520 AT5G18830.2 46.575 73 39 0 162 234 138 210 6.70e-16 80.5
Msa0617520 AT2G47070.2 60.417 48 19 0 187 234 2 49 1.67e-11 67.0

Find 77 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCTGTTTGAGTTTGA+TGG 0.225842 4_2:-68069827 Msa0617520:CDS
CATTGATCCTCTTGGTTTCA+AGG 0.239743 4_2:+68068715 None:intergenic
CAAGGACTTTGGATGTAAAA+TGG 0.260990 4_2:+68068733 None:intergenic
TCAATCTAAAACTCGGTAAA+AGG 0.273664 4_2:-68070274 Msa0617520:CDS
ACTTTCAAATGCAAATTTAC+TGG 0.299044 4_2:+68070397 None:intergenic
TATAACTACCATAGGAGATT+TGG 0.321384 4_2:+68070091 None:intergenic
GCTTCAATAGGCCCTTCTTT+AGG 0.325945 4_2:+68070341 None:intergenic
CCTCTTGGTTTCAAGGACTT+TGG 0.327846 4_2:+68068722 None:intergenic
AGCTTTACCTGCTACATTGC+TGG 0.356873 4_2:+68070045 None:intergenic
ACAAAGATGATTGACTTGAT+TGG 0.359832 4_2:+68069732 None:intergenic
CTCTAGCCTCATCAGAATAT+TGG 0.364549 4_2:-68068291 Msa0617520:CDS
GTGCCAATGTTGTCGTTTAT+AGG 0.365991 4_2:+68068795 None:intergenic
TATGGTAGTTATAGATGGTT+TGG 0.373519 4_2:-68070081 Msa0617520:CDS
GACATTCATGTTAGTCCAAC+TGG 0.375986 4_2:+68068152 None:intergenic
GCAGTCTTTGAATAAGCAAA+TGG 0.377299 4_2:+68068885 None:intergenic
CAAAGATGATTGACTTGATT+GGG 0.382950 4_2:+68069733 None:intergenic
CCGCCTCTCTGCCAGGTTGA+AGG 0.385535 4_2:-68070179 Msa0617520:CDS
TGCTATGGCTTGAGCGGTAC+TGG 0.386033 4_2:+68068336 None:intergenic
TCTCCTATGGTAGTTATAGA+TGG 0.387501 4_2:-68070086 Msa0617520:CDS
CTGACCTGCATTGATCCTCT+TGG 0.395116 4_2:+68068707 None:intergenic
CTCTGCCAGGTTGAAGGTTG+TGG 0.395224 4_2:-68070173 Msa0617520:CDS
CTCCCTCTTCACAGTGGGAA+TGG 0.400387 4_2:-68070634 Msa0617520:CDS
CCAACTGGAAGTGGTTATTC+TGG 0.417567 4_2:-68070503 Msa0617520:CDS
CATCAAACTCAAACAGATCA+TGG 0.444519 4_2:+68069828 None:intergenic
GGTGCATTAGAAGCTTCAAT+AGG 0.450824 4_2:+68070329 None:intergenic
ATGTTCTAGAGAAACGGATT+TGG 0.456583 4_2:+68068372 None:intergenic
AAAATTGAATTACCTAAAGA+AGG 0.460103 4_2:-68070353 Msa0617520:CDS
TCCCTCTTCACAGTGGGAAT+GGG 0.463221 4_2:-68070633 Msa0617520:CDS
ATCTAAATGTTCTAGAGAAA+CGG 0.467117 4_2:+68068366 None:intergenic
TGGTTGTTCGATACGCCAAC+TGG 0.470221 4_2:-68070518 Msa0617520:CDS
CAATCAGTTCATCACCAACT+AGG 0.472722 4_2:-68070433 Msa0617520:CDS
TTCGATACGCCAACTGGAAG+TGG 0.476010 4_2:-68070512 Msa0617520:CDS
ACATTGTAAGTTCTGAGCAT+TGG 0.495722 4_2:+68070202 None:intergenic
AATTCGATACTCCAGTTTGG+TGG 0.496264 4_2:+68070560 None:intergenic
GACGCGTCATCTTTAGTTGT+TGG 0.503063 4_2:-68070239 Msa0617520:CDS
ACAATGTCCGCCTCTCTGCC+AGG 0.504313 4_2:-68070186 Msa0617520:CDS
TCTAATTCGATACTCCAGTT+TGG 0.509220 4_2:+68070557 None:intergenic
TGACAGAAGAGAGGAAGCAC+AGG 0.512878 4_2:+68068420 None:intergenic
CAGTCTTTGAATAAGCAAAT+GGG 0.517662 4_2:+68068886 None:intergenic
GATAGCATAGTTACAGTACT+TGG 0.519974 4_2:+68068771 None:intergenic
CTCTTCTGTCAACCAGTCCG+TGG 0.520942 4_2:-68068408 Msa0617520:CDS
TTTACCATTGGAATCATCAT+GGG 0.526394 4_2:+68070373 None:intergenic
CGTCATCTTTAGTTGTTGGA+AGG 0.527554 4_2:-68070235 Msa0617520:CDS
AGATCATTCCAAATCTCCTA+TGG 0.535644 4_2:-68070099 Msa0617520:CDS
TTGTGGCCTTGACCTTTCAT+CGG 0.541799 4_2:-68070156 Msa0617520:CDS
CGCATATGAATCCCACGGAC+TGG 0.545835 4_2:+68068396 None:intergenic
TAGACCGAGAAAATAATGTC+TGG 0.549417 4_2:-68070538 Msa0617520:CDS
TCCCATTCCCACTGTGAAGA+GGG 0.552746 4_2:+68070632 None:intergenic
AATCAGTTCATCACCAACTA+GGG 0.553568 4_2:-68070432 Msa0617520:CDS
AAACCATCTATAACTACCAT+AGG 0.563385 4_2:+68070083 None:intergenic
CTGACTCGTTGTGTGTGCTA+TGG 0.564583 4_2:+68068321 None:intergenic
ACTGGTGAAAACACGACTCA+AGG 0.568330 4_2:+68068170 None:intergenic
TGAGTTTGAGATCCACATGC+TGG 0.569545 4_2:+68068240 None:intergenic
TCTTCTGTCAACCAGTCCGT+GGG 0.576912 4_2:-68068407 Msa0617520:CDS
CAAAATCTCCCTCTTCACAG+TGG 0.588317 4_2:-68070640 Msa0617520:CDS
AAATTGAATTACCTAAAGAA+GGG 0.590524 4_2:-68070352 Msa0617520:CDS
CAAGGCCACAACCTTCAACC+TGG 0.591428 4_2:+68070168 None:intergenic
CGGTAAAAGGATGAAATTCG+AGG 0.600138 4_2:-68070261 Msa0617520:CDS
CTAGATACACAAGAAAGCAT+TGG 0.604314 4_2:+68070308 None:intergenic
CCATTGCTCAATCTAAAACT+CGG 0.604403 4_2:-68070281 Msa0617520:CDS
ATCCCATTCCCACTGTGAAG+AGG 0.604938 4_2:+68070631 None:intergenic
AAAATCTCCCTCTTCACAGT+GGG 0.605421 4_2:-68070639 Msa0617520:CDS
AAGCCTATAAACGACAACAT+TGG 0.614145 4_2:-68068798 Msa0617520:CDS
GAAACCAAGAGGATCAATGC+AGG 0.614595 4_2:-68068711 Msa0617520:intron
AGTTCCCATGATGATTCCAA+TGG 0.616986 4_2:-68070377 Msa0617520:CDS
CCAGAATAACCACTTCCAGT+TGG 0.626070 4_2:+68070503 None:intergenic
CAATATTCTGATGAGGCTAG+AGG 0.629859 4_2:+68068292 None:intergenic
AATATGTCGATTCCAGCATG+TGG 0.630965 4_2:-68068252 Msa0617520:CDS
CAACCTTCAACCTGGCAGAG+AGG 0.635133 4_2:+68070176 None:intergenic
CCAAAGTCCTTGAAACCAAG+AGG 0.636381 4_2:-68068722 Msa0617520:CDS
TTTGGATGTAAAATGGACAT+CGG 0.653646 4_2:+68068740 None:intergenic
CGGACTGGTTGACAGAAGAG+AGG 0.658311 4_2:+68068411 None:intergenic
ACTGTGCCAATATTCTGATG+AGG 0.658353 4_2:+68068285 None:intergenic
CCTTCAACCTGGCAGAGAGG+CGG 0.660903 4_2:+68070179 None:intergenic
TCTTTGTCTTCGTCACCCTG+TGG 0.667989 4_2:-68069716 Msa0617520:intron
TTGGTCGCATATGAATCCCA+CGG 0.682290 4_2:+68068391 None:intergenic
TGTGTGTGCTATGGCTTGAG+CGG 0.704952 4_2:+68068330 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAACATAATCTATTTATTT+TGG - chr4_2:68070198-68070217 Msa0617520:CDS 10.0%
!!! AGGAAATAATAGTATTATTT+TGG - chr4_2:68068831-68068850 Msa0617520:CDS 15.0%
!!! ATTAAACTTGCTTTTTTTTT+TGG - chr4_2:68068908-68068927 Msa0617520:CDS 15.0%
!! AAACATAAATGAACTAAAAT+TGG + chr4_2:68069801-68069820 None:intergenic 15.0%
!! AAAATTGAATTACCTAAAGA+AGG - chr4_2:68068462-68068481 Msa0617520:CDS 20.0%
!! AAATTGAATTACCTAAAGAA+GGG - chr4_2:68068463-68068482 Msa0617520:CDS 20.0%
!!! CAGTTTTACAATAATGATTA+CGG + chr4_2:68069062-68069081 None:intergenic 20.0%
!!! TTTTAACTTCTTGTAAATAC+TGG - chr4_2:68069408-68069427 Msa0617520:intron 20.0%
!!! GTCATTTTTCATTTATTTCA+TGG - chr4_2:68069430-68069449 Msa0617520:intron 20.0%
!!! CATTTATTTCATGGATTTTT+TGG - chr4_2:68069439-68069458 Msa0617520:intron 20.0%
!!! AGTTGAATTTTTATTACATG+AGG + chr4_2:68069743-68069762 None:intergenic 20.0%
!! TGTAATTGATTAGAGTAATA+TGG - chr4_2:68070258-68070277 Msa0617520:CDS 20.0%
!! ATTTTCAGTAGCTTTTGTAT+CGG + chr4_2:68068223-68068242 None:intergenic 25.0%
!!! TAATTTTTGGTTCTACATCA+GGG - chr4_2:68068343-68068362 Msa0617520:CDS 25.0%
! ACTTTCAAATGCAAATTTAC+TGG + chr4_2:68068421-68068440 None:intergenic 25.0%
!! AGGTAATTCAATTTTACCAT+TGG + chr4_2:68068457-68068476 None:intergenic 25.0%
!!! AACTTGCTTTTTTTTTTGGA+TGG - chr4_2:68068912-68068931 Msa0617520:intron 25.0%
! GAAAAATAAGTATTACCACA+GGG + chr4_2:68069117-68069136 None:intergenic 25.0%
! AGAAAAATAAGTATTACCAC+AGG + chr4_2:68069118-68069137 None:intergenic 25.0%
! AATCAAATAACAAGCAGAAA+CGG + chr4_2:68069160-68069179 None:intergenic 25.0%
!!! TTCTGCTTGTTATTTGATTT+AGG - chr4_2:68069161-68069180 Msa0617520:intron 25.0%
! GATTAAGCATCTTTATCTTT+AGG + chr4_2:68069484-68069503 None:intergenic 25.0%
!!! TGTATAGTGGATTTACTTAA+AGG - chr4_2:68069626-68069645 Msa0617520:intron 25.0%
! GATTAGAAGATAAAAGAGAA+TGG + chr4_2:68069874-68069893 None:intergenic 25.0%
!!! AAGAGAAAATCTTTTGTTTG+GGG + chr4_2:68069996-68070015 None:intergenic 25.0%
!!! CAAGAGAAAATCTTTTGTTT+GGG + chr4_2:68069997-68070016 None:intergenic 25.0%
!! TCAAGAGAAAATCTTTTGTT+TGG + chr4_2:68069998-68070017 None:intergenic 25.0%
! AAACTTAGTAGAAAATCATG+AGG + chr4_2:68070158-68070177 None:intergenic 25.0%
!!! ATCTAAATGTTCTAGAGAAA+CGG + chr4_2:68070452-68070471 None:intergenic 25.0%
! TTTTCAGTAGCTTTTGTATC+GGG + chr4_2:68068222-68068241 None:intergenic 30.0%
AAAAATTACAACCACCAAAC+TGG - chr4_2:68068244-68068263 Msa0617520:CDS 30.0%
! ACAACCAGACATTATTTTCT+CGG + chr4_2:68068284-68068303 None:intergenic 30.0%
!!! CTAATTTTTGGTTCTACATC+AGG - chr4_2:68068342-68068361 Msa0617520:CDS 30.0%
TTTACCATTGGAATCATCAT+GGG + chr4_2:68068445-68068464 None:intergenic 30.0%
! TTTTACCATTGGAATCATCA+TGG + chr4_2:68068446-68068465 None:intergenic 30.0%
! TCAATCTAAAACTCGGTAAA+AGG - chr4_2:68068541-68068560 Msa0617520:intron 30.0%
! AATAATGTTTTGCCGATGAA+AGG + chr4_2:68068674-68068693 None:intergenic 30.0%
TATAACTACCATAGGAGATT+TGG + chr4_2:68068727-68068746 None:intergenic 30.0%
AAACCATCTATAACTACCAT+AGG + chr4_2:68068735-68068754 None:intergenic 30.0%
TATGGTAGTTATAGATGGTT+TGG - chr4_2:68068734-68068753 Msa0617520:CDS 30.0%
TTAACAACGGTATGATTGTT+TGG - chr4_2:68068795-68068814 Msa0617520:CDS 30.0%
TGTTTGGATCTAATTGACAT+AGG - chr4_2:68068811-68068830 Msa0617520:CDS 30.0%
!! CAAAGATGATTGACTTGATT+GGG + chr4_2:68069085-68069104 None:intergenic 30.0%
!! ACAAAGATGATTGACTTGAT+TGG + chr4_2:68069086-68069105 None:intergenic 30.0%
!! TTGATTTAGGTTGTTAAACC+TGG - chr4_2:68069174-68069193 Msa0617520:intron 30.0%
!!! GACTTCTAGATTTTTTTTCC+AGG + chr4_2:68069195-68069214 None:intergenic 30.0%
TTCCTGCTTACTAACTTTAT+AGG - chr4_2:68069314-68069333 Msa0617520:intron 30.0%
TACCTATAAAGTTAGTAAGC+AGG + chr4_2:68069319-68069338 None:intergenic 30.0%
TTATCTTATTCGTGTCACAA+AGG + chr4_2:68069506-68069525 None:intergenic 30.0%
ATAATAACCATGCAGAGTAA+TGG - chr4_2:68069559-68069578 Msa0617520:intron 30.0%
TCATATTAGAGTTGCTAGAA+GGG - chr4_2:68069582-68069601 Msa0617520:intron 30.0%
AAACAGTTATCGTGATTGTT+TGG - chr4_2:68069656-68069675 Msa0617520:intron 30.0%
TGATGTTCCTAAAACTAGAT+TGG - chr4_2:68069694-68069713 Msa0617520:intron 30.0%
AACTAAAATTGGCTACTCTT+CGG + chr4_2:68069790-68069809 None:intergenic 30.0%
CAGTCTTTGAATAAGCAAAT+GGG + chr4_2:68069932-68069951 None:intergenic 30.0%
!! AGAGAAAATCTTTTGTTTGG+GGG + chr4_2:68069995-68070014 None:intergenic 30.0%
TTTGGATGTAAAATGGACAT+CGG + chr4_2:68070078-68070097 None:intergenic 30.0%
TTACTCTAATCAATTACACC+TGG + chr4_2:68070256-68070275 None:intergenic 30.0%
AAATGAATAACACTGTGCAA+GGG + chr4_2:68070305-68070324 None:intergenic 30.0%
TCTAATTCGATACTCCAGTT+TGG + chr4_2:68068261-68068280 None:intergenic 35.0%
TAGACCGAGAAAATAATGTC+TGG - chr4_2:68068277-68068296 Msa0617520:CDS 35.0%
AATCAGTTCATCACCAACTA+GGG - chr4_2:68068383-68068402 Msa0617520:CDS 35.0%
! GTTTTGCTAATATCCCTAGT+TGG + chr4_2:68068399-68068418 None:intergenic 35.0%
! CTAGATACACAAGAAAGCAT+TGG + chr4_2:68068510-68068529 None:intergenic 35.0%
! CCATTGCTCAATCTAAAACT+CGG - chr4_2:68068534-68068553 Msa0617520:intron 35.0%
!! ACATTGTAAGTTCTGAGCAT+TGG + chr4_2:68068616-68068635 None:intergenic 35.0%
AGATCATTCCAAATCTCCTA+TGG - chr4_2:68068716-68068735 Msa0617520:CDS 35.0%
TCTCCTATGGTAGTTATAGA+TGG - chr4_2:68068729-68068748 Msa0617520:CDS 35.0%
CAGGTAAAGCTTCTTAACAA+CGG - chr4_2:68068782-68068801 Msa0617520:CDS 35.0%
CACTTTGCTTAAAATGCTGA+AGG - chr4_2:68068962-68068981 Msa0617520:intron 35.0%
CATCAAACTCAAACAGATCA+TGG + chr4_2:68068990-68069009 None:intergenic 35.0%
! CATGATCTGTTTGAGTTTGA+TGG - chr4_2:68068988-68069007 Msa0617520:intron 35.0%
AAGCAGAAACGGATATAAAC+TGG + chr4_2:68069149-68069168 None:intergenic 35.0%
! AGTGTCTATTGTAACTTGCA+AGG + chr4_2:68069343-68069362 None:intergenic 35.0%
AATATGACCATTACTCTGCA+TGG + chr4_2:68069569-68069588 None:intergenic 35.0%
GTCATATTAGAGTTGCTAGA+AGG - chr4_2:68069581-68069600 Msa0617520:intron 35.0%
!!! ACTCACACCAATCTAGTTTT+AGG + chr4_2:68069704-68069723 None:intergenic 35.0%
GCAGTCTTTGAATAAGCAAA+TGG + chr4_2:68069933-68069952 None:intergenic 35.0%
!! GCTGTTTTGTATGTTTTGCA+AGG + chr4_2:68069967-68069986 None:intergenic 35.0%
AAGCCTATAAACGACAACAT+TGG - chr4_2:68070017-68070036 Msa0617520:intron 35.0%
GATAGCATAGTTACAGTACT+TGG + chr4_2:68070047-68070066 None:intergenic 35.0%
CAAGGACTTTGGATGTAAAA+TGG + chr4_2:68070085-68070104 None:intergenic 35.0%
CAAATGAATAACACTGTGCA+AGG + chr4_2:68070306-68070325 None:intergenic 35.0%
! CTTGCACAGTGTTATTCATT+TGG - chr4_2:68070304-68070323 Msa0617520:CDS 35.0%
! TTTGGTAGAATACCTTTTGC+AGG - chr4_2:68070322-68070341 Msa0617520:CDS 35.0%
AAATCTTCTACACCTGCAAA+AGG + chr4_2:68070337-68070356 None:intergenic 35.0%
!! ATGTTCTAGAGAAACGGATT+TGG + chr4_2:68070446-68070465 None:intergenic 35.0%
AAAATCTCCCTCTTCACAGT+GGG - chr4_2:68068176-68068195 Msa0617520:CDS 40.0%
!! CTTTTGTATCGGGTAATGGT+AGG + chr4_2:68068212-68068231 None:intergenic 40.0%
! GTAGCTTTTGTATCGGGTAA+TGG + chr4_2:68068216-68068235 None:intergenic 40.0%
AATTCGATACTCCAGTTTGG+TGG + chr4_2:68068258-68068277 None:intergenic 40.0%
!!! TCTGGCTCTGAGCTAATTTT+TGG - chr4_2:68068330-68068349 Msa0617520:CDS 40.0%
CAATCAGTTCATCACCAACT+AGG - chr4_2:68068382-68068401 Msa0617520:CDS 40.0%
AGTTCCCATGATGATTCCAA+TGG - chr4_2:68068438-68068457 Msa0617520:CDS 40.0%
GGTGCATTAGAAGCTTCAAT+AGG + chr4_2:68068489-68068508 None:intergenic 40.0%
!!! CCGAGTTTTAGATTGAGCAA+TGG + chr4_2:68068537-68068556 None:intergenic 40.0%
CGGTAAAAGGATGAAATTCG+AGG - chr4_2:68068554-68068573 Msa0617520:intron 40.0%
CGTCATCTTTAGTTGTTGGA+AGG - chr4_2:68068580-68068599 Msa0617520:intron 40.0%
! AGGGATTGCTATTAGAGTTG+TGG - chr4_2:68069601-68069620 Msa0617520:intron 40.0%
! GGGATTGCTATTAGAGTTGT+GGG - chr4_2:68069602-68069621 Msa0617520:intron 40.0%
! GTGCCAATGTTGTCGTTTAT+AGG + chr4_2:68070023-68070042 None:intergenic 40.0%
CATTGATCCTCTTGGTTTCA+AGG + chr4_2:68070103-68070122 None:intergenic 40.0%
CATGGTGGTAGCAATCAATA+TGG + chr4_2:68070224-68070243 None:intergenic 40.0%
TACACCTGGCAATATTTGCA+TGG + chr4_2:68070242-68070261 None:intergenic 40.0%
CAATATTCTGATGAGGCTAG+AGG + chr4_2:68070526-68070545 None:intergenic 40.0%
CTCTAGCCTCATCAGAATAT+TGG - chr4_2:68070524-68070543 Msa0617520:CDS 40.0%
ACTGTGCCAATATTCTGATG+AGG + chr4_2:68070533-68070552 None:intergenic 40.0%
!! AATATGTCGATTCCAGCATG+TGG - chr4_2:68070563-68070582 Msa0617520:CDS 40.0%
CAAAATCTCCCTCTTCACAG+TGG - chr4_2:68068175-68068194 Msa0617520:CDS 45.0%
CCAGAATAACCACTTCCAGT+TGG + chr4_2:68068315-68068334 None:intergenic 45.0%
CCAACTGGAAGTGGTTATTC+TGG - chr4_2:68068312-68068331 Msa0617520:CDS 45.0%
GCTTCAATAGGCCCTTCTTT+AGG + chr4_2:68068477-68068496 None:intergenic 45.0%
GACGCGTCATCTTTAGTTGT+TGG - chr4_2:68068576-68068595 Msa0617520:intron 45.0%
! TTGTGGCCTTGACCTTTCAT+CGG - chr4_2:68068659-68068678 Msa0617520:intron 45.0%
! GTTTTGCCGATGAAAGGTCA+AGG + chr4_2:68068668-68068687 None:intergenic 45.0%
! GATTTTGCCAGCAATGTAGC+AGG - chr4_2:68068763-68068782 Msa0617520:CDS 45.0%
AGCTTTACCTGCTACATTGC+TGG + chr4_2:68068773-68068792 None:intergenic 45.0%
! AGTCTAAGACCATGCTATGC+AGG - chr4_2:68069211-68069230 Msa0617520:intron 45.0%
CAACTATAGCCTGCATAGCA+TGG + chr4_2:68069223-68069242 None:intergenic 45.0%
! TAGAGTTGTGGGCTGTATAG+TGG - chr4_2:68069613-68069632 Msa0617520:intron 45.0%
CCTCTTGGTTTCAAGGACTT+TGG + chr4_2:68070096-68070115 None:intergenic 45.0%
CCAAAGTCCTTGAAACCAAG+AGG - chr4_2:68070093-68070112 Msa0617520:CDS 45.0%
GAAACCAAGAGGATCAATGC+AGG - chr4_2:68070104-68070123 Msa0617520:CDS 45.0%
ACCACCATGCAAATATTGCC+AGG - chr4_2:68070235-68070254 Msa0617520:CDS 45.0%
ACCTGGCAATATTTGCATGG+TGG + chr4_2:68070239-68070258 None:intergenic 45.0%
TTGGTCGCATATGAATCCCA+CGG + chr4_2:68070427-68070446 None:intergenic 45.0%
! TGAGTTTGAGATCCACATGC+TGG + chr4_2:68070578-68070597 None:intergenic 45.0%
ACTGGTGAAAACACGACTCA+AGG + chr4_2:68070648-68070667 None:intergenic 45.0%
! TGAGTCGTGTTTTCACCAGT+TGG - chr4_2:68070648-68070667 Msa0617520:CDS 45.0%
TCCCTCTTCACAGTGGGAAT+GGG - chr4_2:68068182-68068201 Msa0617520:CDS 50.0%
TCCCATTCCCACTGTGAAGA+GGG + chr4_2:68068186-68068205 None:intergenic 50.0%
ATCCCATTCCCACTGTGAAG+AGG + chr4_2:68068187-68068206 None:intergenic 50.0%
TGGTTGTTCGATACGCCAAC+TGG - chr4_2:68068297-68068316 Msa0617520:CDS 50.0%
TTCGATACGCCAACTGGAAG+TGG - chr4_2:68068303-68068322 Msa0617520:CDS 50.0%
TCTTTGTCTTCGTCACCCTG+TGG - chr4_2:68069099-68069118 Msa0617520:intron 50.0%
! CTGACCTGCATTGATCCTCT+TGG + chr4_2:68070111-68070130 None:intergenic 50.0%
! TGACAGAAGAGAGGAAGCAC+AGG + chr4_2:68070398-68070417 None:intergenic 50.0%
TCTTCTGTCAACCAGTCCGT+GGG - chr4_2:68070408-68070427 Msa0617520:CDS 50.0%
TGTGTGTGCTATGGCTTGAG+CGG + chr4_2:68070488-68070507 None:intergenic 50.0%
! CTGACTCGTTGTGTGTGCTA+TGG + chr4_2:68070497-68070516 None:intergenic 50.0%
CTCCCTCTTCACAGTGGGAA+TGG - chr4_2:68068181-68068200 Msa0617520:CDS 55.0%
CAACCTTCAACCTGGCAGAG+AGG + chr4_2:68068642-68068661 None:intergenic 55.0%
! CTCTGCCAGGTTGAAGGTTG+TGG - chr4_2:68068642-68068661 Msa0617520:intron 55.0%
CAAGGCCACAACCTTCAACC+TGG + chr4_2:68068650-68068669 None:intergenic 55.0%
!! CGGACTGGTTGACAGAAGAG+AGG + chr4_2:68070407-68070426 None:intergenic 55.0%
CTCTTCTGTCAACCAGTCCG+TGG - chr4_2:68070407-68070426 Msa0617520:CDS 55.0%
CGCATATGAATCCCACGGAC+TGG + chr4_2:68070422-68070441 None:intergenic 55.0%
TGCTATGGCTTGAGCGGTAC+TGG + chr4_2:68070482-68070501 None:intergenic 55.0%
ACAATGTCCGCCTCTCTGCC+AGG - chr4_2:68068629-68068648 Msa0617520:intron 60.0%
CCTTCAACCTGGCAGAGAGG+CGG + chr4_2:68068639-68068658 None:intergenic 60.0%
! CCGCCTCTCTGCCAGGTTGA+AGG - chr4_2:68068636-68068655 Msa0617520:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 68068162 68070675 68068162 ID=Msa0617520;Name=Msa0617520
chr4_2 mRNA 68068162 68070675 68068162 ID=Msa0617520-mRNA-1;Parent=Msa0617520;Name=Msa0617520-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|0|0|1|1|1|4|0|435
chr4_2 exon 68070053 68070675 68070053 ID=Msa0617520-mRNA-1:exon:19553;Parent=Msa0617520-mRNA-1
chr4_2 exon 68069717 68069853 68069717 ID=Msa0617520-mRNA-1:exon:19552;Parent=Msa0617520-mRNA-1
chr4_2 exon 68068712 68068927 68068712 ID=Msa0617520-mRNA-1:exon:19551;Parent=Msa0617520-mRNA-1
chr4_2 exon 68068162 68068493 68068162 ID=Msa0617520-mRNA-1:exon:19550;Parent=Msa0617520-mRNA-1
chr4_2 CDS 68070053 68070675 68070053 ID=Msa0617520-mRNA-1:cds;Parent=Msa0617520-mRNA-1
chr4_2 CDS 68069717 68069853 68069717 ID=Msa0617520-mRNA-1:cds;Parent=Msa0617520-mRNA-1
chr4_2 CDS 68068712 68068927 68068712 ID=Msa0617520-mRNA-1:cds;Parent=Msa0617520-mRNA-1
chr4_2 CDS 68068162 68068493 68068162 ID=Msa0617520-mRNA-1:cds;Parent=Msa0617520-mRNA-1
Gene Sequence

>Msa0617520

ATGGATTTGGATGCAAAATCTCCCTCTTCACAGTGGGAATGGGATAGCCTACCATTACCCGATACAAAAGCTACTGAAAATAAAAAATTACAACCACCAAACTGGAGTATCGAATTAGACCGAGAAAATAATGTCTGGTTGTTCGATACGCCAACTGGAAGTGGTTATTCTGGCTCTGAGCTAATTTTTGGTTCTACATCAGGGAGTTCGAAATCAGATTCAATCAGTTCATCACCAACTAGGGATATTAGCAAAACCAGTAAATTTGCATTTGAAAGTTCCCATGATGATTCCAATGGTAAAATTGAATTACCTAAAGAAGGGCCTATTGAAGCTTCTAATGCACCAATGCTTTCTTGTGTATCTAGTGAACCATTGCTCAATCTAAAACTCGGTAAAAGGATGAAATTCGAGGACGCGTCATCTTTAGTTGTTGGAAGGAAATGTAAGTCCAATGCTCAGAACTTACAATGTCCGCCTCTCTGCCAGGTTGAAGGTTGTGGCCTTGACCTTTCATCGGCAAAACATTATTATCGCAAACGAAGAGTTTGTGTAGATCATTCCAAATCTCCTATGGTAGTTATAGATGGTTTGGAACGACGATTTTGCCAGCAATGTAGCAGGTTCCATGATCTGTTTGAGTTTGATGGAAAGAAGAAAAGCTGCAGACGACGTCTTTCACAACACAATGCATTGCGCCGTAATCATTATTGTAAAACTGCCCAATCAAGTCAATCATCTTTGTCTTCGTCACCCTGTGATGAGAAGCAACAGATGAACCCATTTGCTTATTCAAAGACTGCTATAAATTTAGCCTTGCAAAACATACAAAACAGCGAGCTCCCCCAAACAAAAGATTTTCTCTTGAAGCCTATAAACGACAACATTGGCACACCAAGTACTGTAACTATGCTATCTGATGATTCCGATGTCCATTTTACATCCAAAGTCCTTGAAACCAAGAGGATCAATGCAGGTGTAGAAGATTTCATTAGCTTCTCTGATACAGATGCTACACAAGACTTCACCTGTGCTTCCTCTCTTCTGTCAACCAGTCCGTGGGATTCATATGCGACCAAATCCGTTTCTCTAGAACATTTAGATTGTACAACCAGTACCGCTCAAGCCATAGCACACACAACGAGTCAGTGTATGCCTCTAGCCTCATCAGAATATTGGCACAGTGTTCTTGATCAATATGTCGATTCCAGCATGTGGATCTCAAACTCAGACTGTGAAGAAAGCGATCATTTTCAAGAATTTCAATGTTCTGAGAACCTTGAGTCGTGTTTTCACCAGTTGGACTAA
Protein sequence

>Msa0617520

MDLDAKSPSSQWEWDSLPLPDTKATENKKLQPPNWSIELDRENNVWLFDTPTGSGYSGSELIFGSTSGSSKSDSISSSPTRDISKTSKFAFESSHDDSNGKIELPKEGPIEASNAPMLSCVSSEPLLNLKLGKRMKFEDASSLVVGRKCKSNAQNLQCPPLCQVEGCGLDLSSAKHYYRKRRVCVDHSKSPMVVIDGLERRFCQQCSRFHDLFEFDGKKKSCRRRLSQHNALRRNHYCKTAQSSQSSLSSSPCDEKQQMNPFAYSKTAINLALQNIQNSELPQTKDFLLKPINDNIGTPSTVTMLSDDSDVHFTSKVLETKRINAGVEDFISFSDTDATQDFTCASSLLSTSPWDSYATKSVSLEHLDCTTSTAQAIAHTTSQCMPLASSEYWHSVLDQYVDSSMWISNSDCEESDHFQEFQCSENLESCFHQLD*