Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0618380 | AET04474.1 | 92.308 | 156 | 12 | 0 | 1 | 156 | 1 | 156 | 1.93e-95 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0618380 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATACCTTCAAATGAAAT+TGG | 0.214941 | 4_2:-69343727 | Msa0618380:CDS |
| CATAAAACTGGCTTGTCTTT+TGG | 0.254968 | 4_2:+69344221 | None:intergenic |
| CTTTGAGGTTTCCCCAGTTT+TGG | 0.256311 | 4_2:+69344177 | None:intergenic |
| ATTCTCAAACTACGTCTCTT+TGG | 0.299791 | 4_2:+69343874 | None:intergenic |
| TCAAACAATAAGGCCTCTTC+TGG | 0.301830 | 4_2:+69343763 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.314815 | 4_2:+69344248 | None:intergenic |
| GATGTTTCTGATTCAGATAA+TGG | 0.318368 | 4_2:-69344062 | Msa0618380:intron |
| CTGTTCGTAACAAGTTCTTT+TGG | 0.340372 | 4_2:+69344131 | None:intergenic |
| TTTGGTCCCTGGCGCCATGC+TGG | 0.374123 | 4_2:+69344239 | None:intergenic |
| GAAAACAACGTTTGAGAGAA+TGG | 0.387976 | 4_2:+69344153 | None:intergenic |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | 4_2:-69344190 | Msa0618380:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | 4_2:+69344209 | None:intergenic |
| CCTTCAAATGAAATTGGCCT+TGG | 0.419265 | 4_2:-69343721 | Msa0618380:CDS |
| CCAAGGCCAATTTCATTTGA+AGG | 0.421742 | 4_2:+69343721 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.436034 | 4_2:-69344246 | Msa0618380:CDS |
| GTTTGAGAGAATGGTCTTTG+AGG | 0.475222 | 4_2:+69344162 | None:intergenic |
| TCTTCTGGAGATGACTCATC+TGG | 0.489997 | 4_2:+69343778 | None:intergenic |
| TCTTGTTGAAACTTCTCCAT+CGG | 0.530728 | 4_2:+69343832 | None:intergenic |
| ATCTGAATCAGAAACATCAA+TGG | 0.539843 | 4_2:+69344067 | None:intergenic |
| TTCTACATAGAAGAACCGTC+TGG | 0.572389 | 4_2:+69343682 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.585142 | 4_2:-69344245 | Msa0618380:CDS |
| AGATGAGTCATCTCCAGAAG+AGG | 0.604525 | 4_2:-69343776 | Msa0618380:CDS |
| GCGGTTCTTCTTAAACCAGA+CGG | 0.605404 | 4_2:-69343697 | Msa0618380:CDS |
| AAATGAAATTGGCCTTGGAG+CGG | 0.608265 | 4_2:-69343716 | Msa0618380:CDS |
| CAATCAGAAGATGACACCGA+TGG | 0.614119 | 4_2:-69343848 | Msa0618380:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | 4_2:-69344189 | Msa0618380:CDS |
| GTTTAAGAAGAACCGCTCCA+AGG | 0.637380 | 4_2:+69343704 | None:intergenic |
| AAAGAACTTGTTACGAACAG+TGG | 0.644687 | 4_2:-69344128 | Msa0618380:CDS |
| CATCACCAGCAACTCCAGCA+TGG | 0.651498 | 4_2:-69344253 | Msa0618380:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | 4_2:-69344188 | Msa0618380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTAAAGAAAAGAGTGAAAA+TGG | + | chr4_2:69343930-69343949 | None:intergenic | 25.0% |
| !!! | TGATATATACTACTTTTGTG+AGG | - | chr4_2:69343951-69343970 | Msa0618380:intron | 25.0% |
| !!! | GATATATACTACTTTTGTGA+GGG | - | chr4_2:69343952-69343971 | Msa0618380:intron | 25.0% |
| !! | GTTCACATTTTCAAACAATA+AGG | + | chr4_2:69344194-69344213 | None:intergenic | 25.0% |
| ! | TTCATACCTTCAAATGAAAT+TGG | - | chr4_2:69344217-69344236 | Msa0618380:CDS | 25.0% |
| ATCTGAATCAGAAACATCAA+TGG | + | chr4_2:69343880-69343899 | None:intergenic | 30.0% | |
| ! | GATGTTTCTGATTCAGATAA+TGG | - | chr4_2:69343882-69343901 | Msa0618380:CDS | 30.0% |
| !!! | CATAAAACTGGCTTGTCTTT+TGG | + | chr4_2:69343726-69343745 | None:intergenic | 35.0% |
| TGGACTAAGATACCAAAACT+GGG | - | chr4_2:69343755-69343774 | Msa0618380:CDS | 35.0% | |
| GAAAACAACGTTTGAGAGAA+TGG | + | chr4_2:69343794-69343813 | None:intergenic | 35.0% | |
| ! | CTGTTCGTAACAAGTTCTTT+TGG | + | chr4_2:69343816-69343835 | None:intergenic | 35.0% |
| !! | AAAGAACTTGTTACGAACAG+TGG | - | chr4_2:69343816-69343835 | Msa0618380:CDS | 35.0% |
| !! | ATATACTACTTTTGTGAGGG+TGG | - | chr4_2:69343955-69343974 | Msa0618380:intron | 35.0% |
| !! | TATACTACTTTTGTGAGGGT+GGG | - | chr4_2:69343956-69343975 | Msa0618380:intron | 35.0% |
| ATTCTCAAACTACGTCTCTT+TGG | + | chr4_2:69344073-69344092 | None:intergenic | 35.0% | |
| TCTTGTTGAAACTTCTCCAT+CGG | + | chr4_2:69344115-69344134 | None:intergenic | 35.0% | |
| CCACATGCATTGCATAAAAC+TGG | + | chr4_2:69343738-69343757 | None:intergenic | 40.0% | |
| !! | CCAGTTTTATGCAATGCATG+TGG | - | chr4_2:69343735-69343754 | Msa0618380:CDS | 40.0% |
| ! | GTGGACTAAGATACCAAAAC+TGG | - | chr4_2:69343754-69343773 | Msa0618380:CDS | 40.0% |
| GGACTAAGATACCAAAACTG+GGG | - | chr4_2:69343756-69343775 | Msa0618380:CDS | 40.0% | |
| GTTTGAGAGAATGGTCTTTG+AGG | + | chr4_2:69343785-69343804 | None:intergenic | 40.0% | |
| TCAAACAATAAGGCCTCTTC+TGG | + | chr4_2:69344184-69344203 | None:intergenic | 40.0% | |
| CCAAGGCCAATTTCATTTGA+AGG | + | chr4_2:69344226-69344245 | None:intergenic | 40.0% | |
| ! | CCTTCAAATGAAATTGGCCT+TGG | - | chr4_2:69344223-69344242 | Msa0618380:CDS | 40.0% |
| !! | AAATGAAATTGGCCTTGGAG+CGG | - | chr4_2:69344228-69344247 | Msa0618380:CDS | 40.0% |
| !!! | CTTTGAGGTTTCCCCAGTTT+TGG | + | chr4_2:69343770-69343789 | None:intergenic | 45.0% |
| CAATCAGAAGATGACACCGA+TGG | - | chr4_2:69344096-69344115 | Msa0618380:CDS | 45.0% | |
| ! | TCTTCTGGAGATGACTCATC+TGG | + | chr4_2:69344169-69344188 | None:intergenic | 45.0% |
| AGATGAGTCATCTCCAGAAG+AGG | - | chr4_2:69344168-69344187 | Msa0618380:CDS | 45.0% | |
| GTTTAAGAAGAACCGCTCCA+AGG | + | chr4_2:69344243-69344262 | None:intergenic | 45.0% | |
| GCGGTTCTTCTTAAACCAGA+CGG | - | chr4_2:69344247-69344266 | Msa0618380:CDS | 45.0% | |
| CATCACCAGCAACTCCAGCA+TGG | - | chr4_2:69343691-69343710 | Msa0618380:CDS | 55.0% | |
| !! | CTGGCTTGTCTTTTGGTCCC+TGG | + | chr4_2:69343719-69343738 | None:intergenic | 55.0% |
| TGGCGCCATGCTGGAGTTGC+TGG | + | chr4_2:69343699-69343718 | None:intergenic | 65.0% | |
| AGCAACTCCAGCATGGCGCC+AGG | - | chr4_2:69343698-69343717 | Msa0618380:CDS | 65.0% | |
| GCAACTCCAGCATGGCGCCA+GGG | - | chr4_2:69343699-69343718 | Msa0618380:CDS | 65.0% | |
| TTTGGTCCCTGGCGCCATGC+TGG | + | chr4_2:69343708-69343727 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 69343684 | 69344282 | 69343684 | ID=Msa0618380;Name=Msa0618380 |
| chr4_2 | mRNA | 69343684 | 69344282 | 69343684 | ID=Msa0618380-mRNA-1;Parent=Msa0618380;Name=Msa0618380-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|1|2|0|156 |
| chr4_2 | exon | 69344063 | 69344282 | 69344063 | ID=Msa0618380-mRNA-1:exon:20050;Parent=Msa0618380-mRNA-1 |
| chr4_2 | exon | 69343684 | 69343934 | 69343684 | ID=Msa0618380-mRNA-1:exon:20049;Parent=Msa0618380-mRNA-1 |
| chr4_2 | CDS | 69344063 | 69344282 | 69344063 | ID=Msa0618380-mRNA-1:cds;Parent=Msa0618380-mRNA-1 |
| chr4_2 | CDS | 69343684 | 69343934 | 69343684 | ID=Msa0618380-mRNA-1:cds;Parent=Msa0618380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
