AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0619130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0619130 MtrunA17_Chr3g0116591 36.464 181 82 6 12 162 345 522 1.04e-21 90.9
Msa0619130 MtrunA17_Chr3g0084691 31.852 135 79 3 40 162 561 694 2.21e-13 67.0
Msa0619130 MtrunA17_Chr3g0078551 44.318 88 47 1 75 162 611 696 2.58e-13 66.6
Msa0619130 MtrunA17_Chr4g0023201 42.647 68 39 0 95 162 29 96 2.80e-13 62.8
Msa0619130 MtrunA17_Chr3g0129391 31.579 133 74 4 47 162 915 1047 3.97e-13 66.2
Msa0619130 MtrunA17_Chr8g0361441 33.333 141 72 5 41 162 541 678 5.44e-13 65.9
Msa0619130 MtrunA17_Chr2g0280791 29.630 135 77 3 45 162 560 693 1.04e-12 65.1
Msa0619130 MtrunA17_Chr3g0078521 47.761 67 35 0 96 162 625 691 1.44e-12 64.3
Msa0619130 MtrunA17_Chr3g0078541 47.761 67 35 0 96 162 636 702 1.44e-12 64.3
Msa0619130 MtrunA17_Chr2g0280681 31.884 138 78 4 41 162 536 673 1.71e-12 64.3
Msa0619130 MtrunA17_Chr3g0078531 47.761 67 35 0 96 162 633 699 2.22e-12 63.9
Msa0619130 MtrunA17_Chr1g0158351 31.206 141 79 4 39 162 4 143 2.32e-12 62.4
Msa0619130 MtrunA17_Chr8g0359691 47.059 68 35 1 95 162 769 835 2.99e-12 63.5
Msa0619130 MtrunA17_Chr3g0084661 29.787 141 80 3 40 162 66 205 3.12e-12 63.2
Msa0619130 MtrunA17_Chr2g0280801 30.000 140 80 3 40 162 561 699 3.80e-12 63.2
Msa0619130 MtrunA17_Chr2g0280831 30.000 140 80 3 40 162 129 267 4.75e-12 62.8
Msa0619130 MtrunA17_Chr7g0236161 30.216 139 81 2 40 162 504 642 5.18e-12 62.8
Msa0619130 MtrunA17_Chr7g0236201 30.216 139 81 2 40 162 504 642 5.21e-12 62.8
Msa0619130 MtrunA17_Chr7g0236121 30.216 139 81 2 40 162 538 676 5.23e-12 62.8
Msa0619130 MtrunA17_Chr8g0361261 33.088 136 71 3 45 162 576 709 6.32e-12 62.8
Msa0619130 MtrunA17_Chr8g0361281 46.269 67 36 0 96 162 619 685 7.27e-12 62.4
Msa0619130 MtrunA17_Chr3g0081071 38.372 86 53 0 77 162 16 101 8.78e-12 61.2
Msa0619130 MtrunA17_Chr3g0084681 29.787 141 81 3 39 162 497 636 8.90e-12 62.0
Msa0619130 MtrunA17_Chr6g0467701 41.096 73 43 0 90 162 133 205 9.22e-12 62.0
Msa0619130 MtrunA17_Chr2g0312651 33.333 129 69 4 50 162 854 981 9.29e-12 62.0
Msa0619130 MtrunA17_Chr4g0023111 42.647 68 39 0 95 162 819 886 9.85e-12 62.0
Msa0619130 MtrunA17_Chr4g0020081 44.776 67 37 0 96 162 617 683 1.06e-11 62.0
Msa0619130 MtrunA17_Chr3g0082941 30.597 134 77 3 45 162 212 345 1.07e-11 62.0
Msa0619130 MtrunA17_Chr1g0151041 33.058 121 65 3 45 149 661 781 1.15e-11 62.0
Msa0619130 MtrunA17_Chr4g0042351 32.061 131 69 2 50 162 497 625 1.26e-11 61.6
Msa0619130 MtrunA17_Chr6g0474901 32.090 134 75 4 45 162 605 738 1.27e-11 61.6
Msa0619130 MtrunA17_Chr2g0280691 30.303 132 76 3 47 162 567 698 1.28e-11 61.6
Msa0619130 MtrunA17_Chr8g0375511 30.303 132 73 4 50 162 883 1014 1.30e-11 61.6
Msa0619130 MtrunA17_Chr2g0280781 31.852 135 74 3 45 162 549 682 1.37e-11 61.6
Msa0619130 MtrunA17_Chr7g0251031 37.931 87 54 0 76 162 13 99 1.57e-11 60.5
Msa0619130 MtrunA17_Chr4g0048581 30.597 134 77 2 45 162 570 703 1.60e-11 61.6
Msa0619130 MtrunA17_Chr6g0467641 41.096 73 43 0 90 162 292 364 1.62e-11 61.2
Msa0619130 MtrunA17_Chr2g0280771 33.051 118 61 3 45 145 521 637 1.69e-11 61.2
Msa0619130 MtrunA17_Chr6g0467681 41.096 73 43 0 90 162 363 435 1.70e-11 61.2
Msa0619130 MtrunA17_Chr2g0323231 30.597 134 77 4 45 162 217 350 1.83e-11 61.2
Msa0619130 MtrunA17_Chr6g0467661 41.096 73 43 0 90 162 363 435 1.84e-11 61.2
Msa0619130 MtrunA17_Chr3g0084411 30.000 140 80 3 40 162 533 671 2.21e-11 61.2
Msa0619130 MtrunA17_Chr3g0098781 44.776 67 37 0 96 162 17 83 2.22e-11 60.1
Msa0619130 MtrunA17_Chr3g0081011 29.710 138 81 3 41 162 374 511 2.31e-11 60.8
Msa0619130 MtrunA17_Chr4g0042391 32.117 137 72 3 44 162 492 625 2.46e-11 60.8
Msa0619130 MtrunA17_Chr2g0315151 40.964 83 48 1 80 162 756 837 2.49e-11 60.8
Msa0619130 MtrunA17_Chr4g0042381 31.298 131 70 3 50 162 497 625 2.55e-11 60.8
Msa0619130 MtrunA17_Chr8g0361251 32.836 134 74 3 45 162 545 678 2.86e-11 60.8
Msa0619130 MtrunA17_Chr5g0428521 44.776 67 37 0 96 162 106 172 3.16e-11 59.3
Msa0619130 MtrunA17_Chr7g0251001 40.260 77 46 0 86 162 156 232 3.22e-11 60.5
Msa0619130 MtrunA17_Chr1g0149381 32.743 113 60 3 51 147 545 657 3.44e-11 60.5
Msa0619130 MtrunA17_Chr5g0428581 44.776 67 37 0 96 162 150 216 3.48e-11 60.1
Msa0619130 MtrunA17_Chr8g0361301 33.088 136 71 3 45 162 545 678 3.50e-11 60.5
Msa0619130 MtrunA17_Chr1g0169841 31.783 129 69 5 50 162 858 983 4.22e-11 60.5
Msa0619130 MtrunA17_Chr7g0251061 41.892 74 43 0 89 162 448 521 4.70e-11 60.1
Msa0619130 MtrunA17_Chr4g0072221 31.496 127 73 2 50 162 111 237 5.04e-11 59.7
Msa0619130 MtrunA17_Chr8g0354391 42.254 71 41 0 92 162 36 106 5.08e-11 59.3
Msa0619130 MtrunA17_Chr3g0081031 44.776 67 37 0 96 162 458 524 5.28e-11 60.1
Msa0619130 MtrunA17_Chr8g0340091 31.690 142 76 3 40 162 565 704 5.31e-11 60.1
Msa0619130 MtrunA17_Chr1g0189871 30.488 164 82 7 29 162 812 973 5.57e-11 60.1
Msa0619130 MtrunA17_Chr3g0084431 29.851 134 76 3 46 162 534 666 6.09e-11 59.7
Msa0619130 MtrunA17_Chr6g0454621 30.075 133 77 4 45 162 871 1002 6.76e-11 59.7
Msa0619130 MtrunA17_Chr1g0206551 44.776 67 37 0 96 162 141 207 7.69e-11 59.3
Msa0619130 MtrunA17_Chr8g0357691 41.791 67 39 0 96 162 584 650 8.59e-11 59.3
Msa0619130 MtrunA17_Chr3g0084491 29.851 134 76 3 46 162 546 678 9.31e-11 59.3
Msa0619130 MtrunA17_Chr8g0354351 30.000 140 82 3 39 162 366 505 9.98e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0619130 AT2G31880.1 37.017 181 81 6 12 162 358 535 1.32e-24 99.4
Msa0619130 AT5G62230.2 33.582 134 72 4 45 162 638 770 1.74e-14 70.5
Msa0619130 AT5G62230.1 33.582 134 72 4 45 162 686 818 1.80e-14 70.5
Msa0619130 AT4G27290.1 35.075 134 71 4 45 162 503 636 2.09e-14 70.1
Msa0619130 AT4G27290.2 35.075 134 71 4 45 162 549 682 1.17e-13 68.2
Msa0619130 AT5G07180.1 33.884 121 64 3 45 149 689 809 1.63e-13 67.8
Msa0619130 AT5G07180.2 33.884 121 64 3 45 149 593 713 1.72e-13 67.4
Msa0619130 AT4G03230.4 31.884 138 78 3 41 162 724 861 3.37e-13 66.6
Msa0619130 AT4G03230.1 31.884 138 78 3 41 162 724 861 3.37e-13 66.6
Msa0619130 AT4G03230.7 31.884 138 78 3 41 162 567 704 4.28e-13 66.2
Msa0619130 AT4G03230.3 31.884 138 78 3 41 162 559 696 4.32e-13 66.2
Msa0619130 AT4G03230.5 31.884 138 78 3 41 162 652 789 4.38e-13 66.2
Msa0619130 AT4G03230.2 31.884 138 78 3 41 162 624 761 4.71e-13 66.2
Msa0619130 AT4G03230.6 31.884 138 78 3 41 162 624 761 4.71e-13 66.2
Msa0619130 AT4G03230.8 31.884 138 78 3 41 162 566 703 5.82e-13 65.9
Msa0619130 AT4G39400.1 31.538 130 72 4 50 162 926 1055 9.31e-13 65.5
Msa0619130 AT4G27300.1 31.159 138 79 4 41 162 534 671 2.74e-12 63.9
Msa0619130 AT2G01950.1 34.091 132 68 5 50 162 881 1012 3.20e-12 63.9
Msa0619130 AT1G56145.3 32.558 129 69 5 50 162 627 753 3.27e-12 63.9
Msa0619130 AT1G56145.1 32.558 129 69 5 50 162 730 856 3.31e-12 63.9
Msa0619130 AT3G02130.2 32.061 131 75 4 45 162 895 1024 3.99e-12 63.5
Msa0619130 AT3G02130.1 32.061 131 75 4 45 162 912 1041 3.99e-12 63.5
Msa0619130 AT1G08590.1 34.109 129 66 4 51 162 772 898 5.63e-12 63.2
Msa0619130 AT1G70250.1 37.500 88 53 1 77 162 542 629 9.80e-12 62.4
Msa0619130 AT1G29720.1 39.773 88 52 1 75 162 756 842 1.83e-11 61.6
Msa0619130 AT1G61360.2 31.579 133 75 2 46 162 456 588 2.73e-11 61.2
Msa0619130 AT1G61360.3 31.579 133 75 2 46 162 456 588 2.73e-11 61.2
Msa0619130 AT1G61360.4 31.579 133 75 2 46 162 456 588 2.73e-11 61.2
Msa0619130 AT1G61360.1 31.579 133 75 2 46 162 537 669 2.77e-11 61.2
Msa0619130 AT1G55610.1 31.061 132 72 4 50 162 902 1033 2.91e-11 61.2
Msa0619130 AT1G55610.2 31.061 132 72 4 50 162 902 1033 2.91e-11 61.2
Msa0619130 AT5G57670.2 42.647 68 39 0 95 162 371 438 3.23e-11 60.8
Msa0619130 AT5G06740.1 30.534 131 73 4 50 162 372 502 3.37e-11 60.8
Msa0619130 AT5G06740.2 30.534 131 73 4 50 162 384 514 3.38e-11 60.8
Msa0619130 AT3G13380.1 30.534 131 73 4 50 162 901 1031 3.46e-11 60.8
Msa0619130 AT4G21410.1 35.075 134 69 4 47 162 272 405 3.66e-11 60.8
Msa0619130 AT4G21410.3 35.075 134 69 4 47 162 397 530 3.73e-11 60.8
Msa0619130 AT5G10520.2 31.111 135 73 3 46 162 192 324 4.55e-11 60.5

Find 34 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAAACTTGCTCCCTTATT+TGG 0.162400 4_2:-70167941 None:intergenic
TTCACCACAGATGCACAATT+TGG 0.235256 4_2:-70167543 None:intergenic
AGACGCAAATGGGTTGGTTT+TGG 0.248616 4_2:+70168742 Msa0619130:intron
CTCTGCAATATTGCGATATA+TGG 0.256763 4_2:+70169131 Msa0619130:CDS
GATGAATTATGCGCGAATTA+TGG 0.257687 4_2:-70168993 None:intergenic
TAGCGGAGATGTACCAAATA+AGG 0.268582 4_2:+70167928 Msa0619130:intron
GGCTCATGTGGATGTATTCT+AGG 0.323360 4_2:-70168972 None:intergenic
AGACTTACAATAACAATGTT+TGG 0.366900 4_2:+70169078 Msa0619130:CDS
TATTCTACTTGATAATGATA+TGG 0.371239 4_2:+70169035 Msa0619130:CDS
AACACGGTTATAGACGCAAA+TGG 0.432646 4_2:+70168731 Msa0619130:intron
TACTTACCTGGATGAATATC+CGG 0.442063 4_2:-70167963 None:intergenic
ATACTAATTGTACTATGAGC+AGG 0.455348 4_2:+70167510 None:intergenic
AGCGGAGATGTACCAAATAA+GGG 0.483962 4_2:+70167929 Msa0619130:intron
TGTGGTGAATGTTTCACCTT+GGG 0.502016 4_2:+70167557 Msa0619130:CDS
CTGTGGTGAATGTTTCACCT+TGG 0.505122 4_2:+70167556 Msa0619130:CDS
ACACGGTTATAGACGCAAAT+GGG 0.506140 4_2:+70168732 Msa0619130:intron
AATTCGGAGATTACCACTGT+TGG 0.517513 4_2:+70168773 Msa0619130:CDS
TGTGCGAGTACATGAAGAAC+GGG 0.523879 4_2:+70168856 Msa0619130:CDS
TAGATTTGACAGTCATTACA+TGG 0.534792 4_2:+70168833 Msa0619130:intron
GTACTATGAGCAGGGAACTC+AGG 0.555188 4_2:+70167519 None:intergenic
ATTGCAGTTGGTATCGTGGA+TGG 0.566751 4_2:+70168949 Msa0619130:intron
GGTTATAGACGCAAATGGGT+TGG 0.574153 4_2:+70168736 Msa0619130:intron
TTCACCTTGGGCAATAATTG+TGG 0.583281 4_2:+70167569 Msa0619130:CDS
AGGAGTTTGACTAGTTGTCA+AGG 0.597128 4_2:+70168920 Msa0619130:CDS
TAAGATTGCAGTTGGTATCG+TGG 0.605267 4_2:+70168945 Msa0619130:intron
GCAACCACAATTATTGCCCA+AGG 0.608188 4_2:-70167573 None:intergenic
TACTAATTGTACTATGAGCA+GGG 0.613761 4_2:+70167511 None:intergenic
TGCGCGAATTATGGCTCATG+TGG 0.623387 4_2:-70168984 None:intergenic
GTGTGCGAGTACATGAAGAA+CGG 0.628202 4_2:+70168855 Msa0619130:CDS
CATATATCGCAATATTGCAG+AGG 0.635792 4_2:-70169130 None:intergenic
AGGTCCAAATTGTGCATCTG+TGG 0.637059 4_2:+70167539 Msa0619130:CDS
TTATCAAGTAGAATATTCGT+AGG 0.637865 4_2:-70169027 None:intergenic
CTGTTGGTCTGATTCGACAT+CGG 0.649803 4_2:+70168789 Msa0619130:CDS
ATGTCGAATCAGACCAACAG+TGG 0.658806 4_2:-70168786 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCATAATTTCTATAAAA+TGG + chr4_2:70167663-70167682 Msa0619130:intron 10.0%
!! ATATGTAAGATAATTTAATT+TGG + chr4_2:70167752-70167771 Msa0619130:intron 10.0%
!! TTGTTAAATAAAAGATTTAA+GGG - chr4_2:70168102-70168121 None:intergenic 10.0%
!! TTTGTTAAATAAAAGATTTA+AGG - chr4_2:70168103-70168122 None:intergenic 10.0%
!!! TTTAGTTTTCTATATTTATT+TGG - chr4_2:70168534-70168553 None:intergenic 10.0%
!!! GTTTTTTATATTATTGTTTT+TGG - chr4_2:70168578-70168597 None:intergenic 10.0%
!! AAATCATAATTTCTATAAAA+TGG + chr4_2:70167663-70167682 Msa0619130:intron 10.0%
!! ATATGTAAGATAATTTAATT+TGG + chr4_2:70167752-70167771 Msa0619130:intron 10.0%
!! TTGTTAAATAAAAGATTTAA+GGG - chr4_2:70168102-70168121 None:intergenic 10.0%
!! TTTGTTAAATAAAAGATTTA+AGG - chr4_2:70168103-70168122 None:intergenic 10.0%
!!! TTTAGTTTTCTATATTTATT+TGG - chr4_2:70168534-70168553 None:intergenic 10.0%
!!! GTTTTTTATATTATTGTTTT+TGG - chr4_2:70168578-70168597 None:intergenic 10.0%
!!! TTGGATACAATTTTTTTTAT+AGG - chr4_2:70167901-70167920 None:intergenic 15.0%
!!! TTGGATACAATTTTTTTTAT+AGG - chr4_2:70167901-70167920 None:intergenic 15.0%
!! TAGGAAATAACAAAATTATG+AGG + chr4_2:70167639-70167658 Msa0619130:intron 20.0%
!!! AATATTTTTGCTTCATGTTT+AGG + chr4_2:70167853-70167872 Msa0619130:intron 20.0%
!!! ATATTTTTGCTTCATGTTTA+GGG + chr4_2:70167854-70167873 Msa0619130:intron 20.0%
!! TTGTATCCAATTATTTATAG+CGG + chr4_2:70167911-70167930 Msa0619130:intron 20.0%
!!! TTTTTTTCTCTTTACTGTTT+TGG - chr4_2:70168038-70168057 None:intergenic 20.0%
!! TTTATTTGGATATTTATGCA+TGG - chr4_2:70168520-70168539 None:intergenic 20.0%
!! CATGAAATTAAAGATTAACA+CGG + chr4_2:70168715-70168734 Msa0619130:intron 20.0%
!!! GGTTTTGGAAAAAATTAATT+CGG + chr4_2:70168757-70168776 Msa0619130:CDS 20.0%
!! TGTTGAAAAAAAATTGAAAG+AGG + chr4_2:70168892-70168911 Msa0619130:CDS 20.0%
!!! TATTCTACTTGATAATGATA+TGG + chr4_2:70169035-70169054 Msa0619130:CDS 20.0%
!! TAGGAAATAACAAAATTATG+AGG + chr4_2:70167639-70167658 Msa0619130:intron 20.0%
!!! AATATTTTTGCTTCATGTTT+AGG + chr4_2:70167853-70167872 Msa0619130:intron 20.0%
!!! ATATTTTTGCTTCATGTTTA+GGG + chr4_2:70167854-70167873 Msa0619130:intron 20.0%
!! TTGTATCCAATTATTTATAG+CGG + chr4_2:70167911-70167930 Msa0619130:intron 20.0%
!!! TTTTTTTCTCTTTACTGTTT+TGG - chr4_2:70168038-70168057 None:intergenic 20.0%
!! TTTATTTGGATATTTATGCA+TGG - chr4_2:70168520-70168539 None:intergenic 20.0%
!! CATGAAATTAAAGATTAACA+CGG + chr4_2:70168715-70168734 Msa0619130:intron 20.0%
!!! GGTTTTGGAAAAAATTAATT+CGG + chr4_2:70168757-70168776 Msa0619130:CDS 20.0%
!! TGTTGAAAAAAAATTGAAAG+AGG + chr4_2:70168892-70168911 Msa0619130:CDS 20.0%
!!! TATTCTACTTGATAATGATA+TGG + chr4_2:70169035-70169054 Msa0619130:CDS 20.0%
! CAAATTAAATTTCCTGTCTA+AGG - chr4_2:70167604-70167623 None:intergenic 25.0%
!!! GGAAATTTAATTTGTTACCA+AGG + chr4_2:70167610-70167629 Msa0619130:intron 25.0%
! TTTGTTACCAAGGTTAAAAT+AGG + chr4_2:70167620-70167639 Msa0619130:intron 25.0%
!!! GTTATTTCCTATTTTAACCT+TGG - chr4_2:70167630-70167649 None:intergenic 25.0%
!!! GATAATTTAATTTGGTGTGT+AGG + chr4_2:70167760-70167779 Msa0619130:intron 25.0%
! AATTGCAACATTAGTAATGA+TGG + chr4_2:70167829-70167848 Msa0619130:intron 25.0%
! TTATAGGTCAAATGTATTCA+GGG - chr4_2:70167885-70167904 None:intergenic 25.0%
! TTTATAGGTCAAATGTATTC+AGG - chr4_2:70167886-70167905 None:intergenic 25.0%
! ATTTAAGGGACTAAATTTGA+AGG - chr4_2:70168088-70168107 None:intergenic 25.0%
! TTACCACTTCACTAATATTA+TGG - chr4_2:70168158-70168177 None:intergenic 25.0%
! ATAATATTAGTGAAGTGGTA+AGG + chr4_2:70168157-70168176 Msa0619130:intron 25.0%
!!! CATTTTATTTAGCTCGAATT+CGG + chr4_2:70168283-70168302 Msa0619130:intron 25.0%
!! AATTCGGTTTGTTTAAAGTT+AGG + chr4_2:70168299-70168318 Msa0619130:intron 25.0%
! AAAAATTGAAAGAGGAGAAA+AGG + chr4_2:70168900-70168919 Msa0619130:CDS 25.0%
! TTATCAAGTAGAATATTCGT+AGG - chr4_2:70169030-70169049 None:intergenic 25.0%
! AGACTTACAATAACAATGTT+TGG + chr4_2:70169078-70169097 Msa0619130:CDS 25.0%
! CAAATTAAATTTCCTGTCTA+AGG - chr4_2:70167604-70167623 None:intergenic 25.0%
!!! GGAAATTTAATTTGTTACCA+AGG + chr4_2:70167610-70167629 Msa0619130:intron 25.0%
! TTTGTTACCAAGGTTAAAAT+AGG + chr4_2:70167620-70167639 Msa0619130:intron 25.0%
!!! GTTATTTCCTATTTTAACCT+TGG - chr4_2:70167630-70167649 None:intergenic 25.0%
!!! GATAATTTAATTTGGTGTGT+AGG + chr4_2:70167760-70167779 Msa0619130:intron 25.0%
! AATTGCAACATTAGTAATGA+TGG + chr4_2:70167829-70167848 Msa0619130:intron 25.0%
! TTATAGGTCAAATGTATTCA+GGG - chr4_2:70167885-70167904 None:intergenic 25.0%
! TTTATAGGTCAAATGTATTC+AGG - chr4_2:70167886-70167905 None:intergenic 25.0%
! ATTTAAGGGACTAAATTTGA+AGG - chr4_2:70168088-70168107 None:intergenic 25.0%
! TTACCACTTCACTAATATTA+TGG - chr4_2:70168158-70168177 None:intergenic 25.0%
! ATAATATTAGTGAAGTGGTA+AGG + chr4_2:70168157-70168176 Msa0619130:intron 25.0%
!!! CATTTTATTTAGCTCGAATT+CGG + chr4_2:70168283-70168302 Msa0619130:intron 25.0%
!! AATTCGGTTTGTTTAAAGTT+AGG + chr4_2:70168299-70168318 Msa0619130:intron 25.0%
! AAAAATTGAAAGAGGAGAAA+AGG + chr4_2:70168900-70168919 Msa0619130:CDS 25.0%
! TTATCAAGTAGAATATTCGT+AGG - chr4_2:70169030-70169049 None:intergenic 25.0%
! AGACTTACAATAACAATGTT+TGG + chr4_2:70169078-70169097 Msa0619130:CDS 25.0%
ACATCTCCGCTATAAATAAT+TGG - chr4_2:70167920-70167939 None:intergenic 30.0%
ACTCCATAATATTAGTGAAG+TGG + chr4_2:70168152-70168171 Msa0619130:intron 30.0%
GTATGAATTCCTCCTAAATA+TGG + chr4_2:70168185-70168204 Msa0619130:intron 30.0%
!! ACTCATTTCTAGCCATATTT+AGG - chr4_2:70168200-70168219 None:intergenic 30.0%
GTTCAGTTTATAACTTGACT+CGG + chr4_2:70168248-70168267 Msa0619130:intron 30.0%
TTCAGTTTATAACTTGACTC+GGG + chr4_2:70168249-70168268 Msa0619130:intron 30.0%
TAGATTTGACAGTCATTACA+TGG + chr4_2:70168833-70168852 Msa0619130:intron 30.0%
ACATCTCCGCTATAAATAAT+TGG - chr4_2:70167920-70167939 None:intergenic 30.0%
ACTCCATAATATTAGTGAAG+TGG + chr4_2:70168152-70168171 Msa0619130:intron 30.0%
GTATGAATTCCTCCTAAATA+TGG + chr4_2:70168185-70168204 Msa0619130:intron 30.0%
!! ACTCATTTCTAGCCATATTT+AGG - chr4_2:70168200-70168219 None:intergenic 30.0%
GTTCAGTTTATAACTTGACT+CGG + chr4_2:70168248-70168267 Msa0619130:intron 30.0%
TTCAGTTTATAACTTGACTC+GGG + chr4_2:70168249-70168268 Msa0619130:intron 30.0%
TAGATTTGACAGTCATTACA+TGG + chr4_2:70168833-70168852 Msa0619130:intron 30.0%
TACTTACCTGGATGAATATC+CGG - chr4_2:70167966-70167985 None:intergenic 35.0%
TGATACAAAGACTACTTACC+TGG - chr4_2:70167978-70167997 None:intergenic 35.0%
TAAGGGACTAAATTTGAAGG+TGG - chr4_2:70168085-70168104 None:intergenic 35.0%
!! CATTTCTAGCCATATTTAGG+AGG - chr4_2:70168197-70168216 None:intergenic 35.0%
TGACTCAACACGATAAAAGT+CGG + chr4_2:70168226-70168245 Msa0619130:intron 35.0%
! AGCTCATAATTTGACTCGTT+AGG + chr4_2:70168325-70168344 Msa0619130:intron 35.0%
TCAAGGTATAAGATTGCAGT+TGG + chr4_2:70168937-70168956 Msa0619130:intron 35.0%
GATGAATTATGCGCGAATTA+TGG - chr4_2:70168996-70169015 None:intergenic 35.0%
CATATATCGCAATATTGCAG+AGG - chr4_2:70169133-70169152 None:intergenic 35.0%
CTCTGCAATATTGCGATATA+TGG + chr4_2:70169131-70169150 Msa0619130:CDS 35.0%
TACTTACCTGGATGAATATC+CGG - chr4_2:70167966-70167985 None:intergenic 35.0%
TGATACAAAGACTACTTACC+TGG - chr4_2:70167978-70167997 None:intergenic 35.0%
TAAGGGACTAAATTTGAAGG+TGG - chr4_2:70168085-70168104 None:intergenic 35.0%
!! CATTTCTAGCCATATTTAGG+AGG - chr4_2:70168197-70168216 None:intergenic 35.0%
TGACTCAACACGATAAAAGT+CGG + chr4_2:70168226-70168245 Msa0619130:intron 35.0%
! AGCTCATAATTTGACTCGTT+AGG + chr4_2:70168325-70168344 Msa0619130:intron 35.0%
TCAAGGTATAAGATTGCAGT+TGG + chr4_2:70168937-70168956 Msa0619130:intron 35.0%
GATGAATTATGCGCGAATTA+TGG - chr4_2:70168996-70169015 None:intergenic 35.0%
CATATATCGCAATATTGCAG+AGG - chr4_2:70169133-70169152 None:intergenic 35.0%
CTCTGCAATATTGCGATATA+TGG + chr4_2:70169131-70169150 Msa0619130:CDS 35.0%
TTCACCACAGATGCACAATT+TGG - chr4_2:70167546-70167565 None:intergenic 40.0%
TGTGGTGAATGTTTCACCTT+GGG + chr4_2:70167557-70167576 Msa0619130:CDS 40.0%
TTCACCTTGGGCAATAATTG+TGG + chr4_2:70167569-70167588 Msa0619130:CDS 40.0%
TAGCGGAGATGTACCAAATA+AGG + chr4_2:70167928-70167947 Msa0619130:intron 40.0%
AGCGGAGATGTACCAAATAA+GGG + chr4_2:70167929-70167948 Msa0619130:intron 40.0%
! GCAAAACTTGCTCCCTTATT+TGG - chr4_2:70167944-70167963 None:intergenic 40.0%
! AATAAGGGAGCAAGTTTTGC+CGG + chr4_2:70167944-70167963 Msa0619130:CDS 40.0%
AACACGGTTATAGACGCAAA+TGG + chr4_2:70168731-70168750 Msa0619130:intron 40.0%
ACACGGTTATAGACGCAAAT+GGG + chr4_2:70168732-70168751 Msa0619130:intron 40.0%
AATTCGGAGATTACCACTGT+TGG + chr4_2:70168773-70168792 Msa0619130:CDS 40.0%
! AGGAGTTTGACTAGTTGTCA+AGG + chr4_2:70168920-70168939 Msa0619130:CDS 40.0%
! TAAGATTGCAGTTGGTATCG+TGG + chr4_2:70168945-70168964 Msa0619130:intron 40.0%
TTCACCACAGATGCACAATT+TGG - chr4_2:70167546-70167565 None:intergenic 40.0%
TGTGGTGAATGTTTCACCTT+GGG + chr4_2:70167557-70167576 Msa0619130:CDS 40.0%
TTCACCTTGGGCAATAATTG+TGG + chr4_2:70167569-70167588 Msa0619130:CDS 40.0%
TAGCGGAGATGTACCAAATA+AGG + chr4_2:70167928-70167947 Msa0619130:intron 40.0%
AGCGGAGATGTACCAAATAA+GGG + chr4_2:70167929-70167948 Msa0619130:intron 40.0%
! GCAAAACTTGCTCCCTTATT+TGG - chr4_2:70167944-70167963 None:intergenic 40.0%
! AATAAGGGAGCAAGTTTTGC+CGG + chr4_2:70167944-70167963 Msa0619130:CDS 40.0%
AACACGGTTATAGACGCAAA+TGG + chr4_2:70168731-70168750 Msa0619130:intron 40.0%
ACACGGTTATAGACGCAAAT+GGG + chr4_2:70168732-70168751 Msa0619130:intron 40.0%
AATTCGGAGATTACCACTGT+TGG + chr4_2:70168773-70168792 Msa0619130:CDS 40.0%
! AGGAGTTTGACTAGTTGTCA+AGG + chr4_2:70168920-70168939 Msa0619130:CDS 40.0%
! TAAGATTGCAGTTGGTATCG+TGG + chr4_2:70168945-70168964 Msa0619130:intron 40.0%
AGGTCCAAATTGTGCATCTG+TGG + chr4_2:70167539-70167558 Msa0619130:CDS 45.0%
CTGTGGTGAATGTTTCACCT+TGG + chr4_2:70167556-70167575 Msa0619130:CDS 45.0%
GCAACCACAATTATTGCCCA+AGG - chr4_2:70167576-70167595 None:intergenic 45.0%
! GTTTTGCCGGATATTCATCC+AGG + chr4_2:70167957-70167976 Msa0619130:CDS 45.0%
GGTTATAGACGCAAATGGGT+TGG + chr4_2:70168736-70168755 Msa0619130:intron 45.0%
!!! AGACGCAAATGGGTTGGTTT+TGG + chr4_2:70168742-70168761 Msa0619130:intron 45.0%
ATGTCGAATCAGACCAACAG+TGG - chr4_2:70168789-70168808 None:intergenic 45.0%
! CTGTTGGTCTGATTCGACAT+CGG + chr4_2:70168789-70168808 Msa0619130:CDS 45.0%
ATTCGACATCGGAACGTTCT+TGG + chr4_2:70168800-70168819 Msa0619130:intron 45.0%
GTGTGCGAGTACATGAAGAA+CGG + chr4_2:70168855-70168874 Msa0619130:CDS 45.0%
TGTGCGAGTACATGAAGAAC+GGG + chr4_2:70168856-70168875 Msa0619130:CDS 45.0%
! ATTGCAGTTGGTATCGTGGA+TGG + chr4_2:70168949-70168968 Msa0619130:intron 45.0%
GGCTCATGTGGATGTATTCT+AGG - chr4_2:70168975-70168994 None:intergenic 45.0%
AGGTCCAAATTGTGCATCTG+TGG + chr4_2:70167539-70167558 Msa0619130:CDS 45.0%
CTGTGGTGAATGTTTCACCT+TGG + chr4_2:70167556-70167575 Msa0619130:CDS 45.0%
GCAACCACAATTATTGCCCA+AGG - chr4_2:70167576-70167595 None:intergenic 45.0%
! GTTTTGCCGGATATTCATCC+AGG + chr4_2:70167957-70167976 Msa0619130:CDS 45.0%
GGTTATAGACGCAAATGGGT+TGG + chr4_2:70168736-70168755 Msa0619130:intron 45.0%
!!! AGACGCAAATGGGTTGGTTT+TGG + chr4_2:70168742-70168761 Msa0619130:intron 45.0%
ATGTCGAATCAGACCAACAG+TGG - chr4_2:70168789-70168808 None:intergenic 45.0%
! CTGTTGGTCTGATTCGACAT+CGG + chr4_2:70168789-70168808 Msa0619130:CDS 45.0%
ATTCGACATCGGAACGTTCT+TGG + chr4_2:70168800-70168819 Msa0619130:intron 45.0%
GTGTGCGAGTACATGAAGAA+CGG + chr4_2:70168855-70168874 Msa0619130:CDS 45.0%
TGTGCGAGTACATGAAGAAC+GGG + chr4_2:70168856-70168875 Msa0619130:CDS 45.0%
! ATTGCAGTTGGTATCGTGGA+TGG + chr4_2:70168949-70168968 Msa0619130:intron 45.0%
GGCTCATGTGGATGTATTCT+AGG - chr4_2:70168975-70168994 None:intergenic 45.0%
TGGTTGCAAGCACCTTAGAC+AGG + chr4_2:70167589-70167608 Msa0619130:intron 50.0%
TGCGCGAATTATGGCTCATG+TGG - chr4_2:70168987-70169006 None:intergenic 50.0%
TGGTTGCAAGCACCTTAGAC+AGG + chr4_2:70167589-70167608 Msa0619130:intron 50.0%
TGCGCGAATTATGGCTCATG+TGG - chr4_2:70168987-70169006 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 70167524 70169161 70167524 ID=Msa0619130;Name=Msa0619130
chr4_2 mRNA 70167524 70169161 70167524 ID=Msa0619130-mRNA-1;Parent=Msa0619130;Name=Msa0619130-mRNA-1;_AED=0.37;_eAED=0.79;_QI=0|0|0|1|0|0|5|0|162
chr4_2 exon 70167524 70167590 70167524 ID=Msa0619130-mRNA-1:exon:20408;Parent=Msa0619130-mRNA-1
chr4_2 exon 70167931 70167978 70167931 ID=Msa0619130-mRNA-1:exon:20409;Parent=Msa0619130-mRNA-1
chr4_2 exon 70168744 70168814 70168744 ID=Msa0619130-mRNA-1:exon:20410;Parent=Msa0619130-mRNA-1
chr4_2 exon 70168845 70168941 70168845 ID=Msa0619130-mRNA-1:exon:20411;Parent=Msa0619130-mRNA-1
chr4_2 exon 70168956 70169161 70168956 ID=Msa0619130-mRNA-1:exon:20412;Parent=Msa0619130-mRNA-1
chr4_2 CDS 70167524 70167590 70167524 ID=Msa0619130-mRNA-1:cds;Parent=Msa0619130-mRNA-1
chr4_2 CDS 70167931 70167978 70167931 ID=Msa0619130-mRNA-1:cds;Parent=Msa0619130-mRNA-1
chr4_2 CDS 70168744 70168814 70168744 ID=Msa0619130-mRNA-1:cds;Parent=Msa0619130-mRNA-1
chr4_2 CDS 70168845 70168941 70168845 ID=Msa0619130-mRNA-1:cds;Parent=Msa0619130-mRNA-1
chr4_2 CDS 70168956 70169161 70168956 ID=Msa0619130-mRNA-1:cds;Parent=Msa0619130-mRNA-1
Gene Sequence

>Msa0619130

ATGAGCAGGGAACTCAGGTCCAAATTGTGCATCTGTGGTGAATGTTTCACCTTGGGCAATAATTGTGCGGAGATGTACCAAATAAGGGAGCAAGTTTTGCCGGATATTCATCCAGACGCAAATGGGTTGGTTTTGGAAAAAATTAATTCGGAGATTACCACTGTTGGTCTGATTCGACATCGGAACTCATTACATGGTGTGCGAGTACATGAAGAACGGGAGTTTGCACAATATGTTGAAAAAAAATTGAAAGAGGAGAAAAGGAGTTTGACTAGTTGTCAAGTTGGTATCGTGGATGGCCTAGAATACATCCACATGAGCCATAATTCGCGCATAATTCATCGAGATCTCAAGCCTACGAATATTCTACTTGATAATGATATGGAAGCTAAGATTACAAATTTTAGACTTACAATAACAATGTTTGGTGCGCAGACTCATAGAAGTGTTGATCATGCCTCTGCAATATTGCGATATATGGCGCCATAG
Protein sequence

>Msa0619130

MSRELRSKLCICGECFTLGNNCAEMYQIREQVLPDIHPDANGLVLEKINSEITTVGLIRHRNSLHGVRVHEEREFAQYVEKKLKEEKRSLTSCQVGIVDGLEYIHMSHNSRIIHRDLKPTNILLDNDMEAKITNFRLTITMFGAQTHRSVDHASAILRYMAP*