AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0621520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621520 MtrunA17_Chr2g0287061 98.423 761 12 0 12 772 1 761 0.0 1568
Msa0621520 MtrunA17_Chr5g0448761 37.043 629 342 14 192 768 58 684 6.38e-130 405
Msa0621520 MtrunA17_Chr5g0440451 37.239 623 341 11 193 768 42 661 2.48e-122 389
Msa0621520 MtrunA17_Chr5g0440451 37.778 90 56 0 39 128 42 131 3.89e-12 70.1
Msa0621520 MtrunA17_Chr5g0448511 42.986 442 241 6 337 768 16 456 8.69e-122 376
Msa0621520 MtrunA17_Chr1g0202341 35.191 628 349 13 192 768 49 669 2.47e-120 377
Msa0621520 MtrunA17_Chr6g0458391 31.150 748 429 15 7 711 60 764 3.59e-110 353
Msa0621520 MtrunA17_Chr6g0458391 29.480 173 99 4 130 282 22 191 2.50e-16 83.6
Msa0621520 MtrunA17_Chr3g0118611 40.991 444 254 6 332 769 118 559 1.67e-102 333
Msa0621520 MtrunA17_Chr1g0207551 34.744 567 327 8 186 712 26 589 9.19e-101 329
Msa0621520 MtrunA17_Chr2g0291581 31.960 607 375 12 194 764 71 675 1.43e-96 315
Msa0621520 MtrunA17_Chr2g0291581 32.759 116 68 2 22 128 54 168 4.79e-11 66.2
Msa0621520 MtrunA17_Chr2g0329031 33.607 610 362 14 193 767 13 614 3.27e-96 315
Msa0621520 MtrunA17_Chr3g0080351 30.533 619 369 13 192 766 74 675 3.02e-89 299
Msa0621520 MtrunA17_Chr5g0441581 31.777 557 343 10 189 710 27 581 7.98e-88 291
Msa0621520 MtrunA17_Chr8g0363841 31.514 568 339 13 195 716 18 581 3.20e-83 278
Msa0621520 MtrunA17_Chr1g0203721 29.822 617 380 11 196 763 65 677 8.61e-83 282
Msa0621520 MtrunA17_Chr1g0203721 34.400 125 69 1 42 153 65 189 6.27e-12 69.3
Msa0621520 MtrunA17_Chr2g0324011 29.422 571 353 14 193 718 9 574 4.65e-79 265
Msa0621520 MtrunA17_Chr2g0324011 36.036 111 53 4 35 130 5 112 8.39e-11 65.5
Msa0621520 MtrunA17_Chr2g0296781 31.681 565 319 15 200 710 3 554 2.94e-77 264
Msa0621520 MtrunA17_Chr2g0291591 31.712 514 302 10 159 630 33 539 3.14e-77 259
Msa0621520 MtrunA17_Chr1g0203051 29.217 575 349 15 168 699 62 621 2.84e-76 259
Msa0621520 MtrunA17_Chr6g0477261 36.641 393 222 8 337 710 59 443 1.09e-74 255
Msa0621520 MtrunA17_Chr1g0196541 29.910 555 340 14 200 709 3 553 6.06e-72 249
Msa0621520 MtrunA17_Chr3g0082121 29.126 618 380 18 194 766 43 647 1.93e-71 247
Msa0621520 MtrunA17_Chr2g0287651 28.521 568 337 13 199 712 80 632 2.45e-67 239
Msa0621520 MtrunA17_Chr1g0198761 28.664 614 362 17 195 741 48 652 1.98e-57 209
Msa0621520 MtrunA17_Chr2g0276841 26.445 571 351 15 194 709 70 626 2.29e-57 208
Msa0621520 MtrunA17_Chr2g0294841 26.003 573 365 13 187 707 38 603 2.45e-50 189
Msa0621520 MtrunA17_Chr2g0290391 26.770 579 346 18 192 709 49 610 1.06e-49 186
Msa0621520 MtrunA17_Chr1g0164591 32.645 242 154 5 315 550 72 310 4.09e-40 150
Msa0621520 MtrunA17_Chr5g0431001 27.765 443 246 13 176 555 40 471 8.27e-40 154
Msa0621520 MtrunA17_Chr7g0258861 31.058 293 162 8 193 446 9 300 9.72e-31 123
Msa0621520 MtrunA17_Chr7g0258861 30.233 129 65 3 40 154 10 127 2.69e-11 65.5
Msa0621520 MtrunA17_Chr2g0329421 42.157 102 47 1 193 282 26 127 1.48e-21 93.6
Msa0621520 MtrunA17_Chr2g0329421 41.176 102 48 1 39 128 26 127 4.65e-18 83.6
Msa0621520 MtrunA17_Chr2g0308841 25.893 224 159 4 493 711 5 226 1.68e-21 95.1
Msa0621520 MtrunA17_Chr4g0033611 42.202 109 51 1 193 289 75 183 1.85e-21 94.7
Msa0621520 MtrunA17_Chr4g0033611 37.255 102 52 1 39 128 75 176 5.94e-16 78.6
Msa0621520 MtrunA17_Chr7g0233911 34.091 176 90 4 39 189 33 207 4.40e-19 86.7
Msa0621520 MtrunA17_Chr7g0233911 44.231 104 44 2 193 282 33 136 7.79e-18 83.2
Msa0621520 MtrunA17_Chr2g0329361 36.735 147 70 3 37 166 58 198 6.75e-19 87.0
Msa0621520 MtrunA17_Chr2g0329361 33.019 106 56 1 192 282 59 164 2.20e-13 70.9
Msa0621520 MtrunA17_Chr3g0078041 35.075 134 73 1 39 158 49 182 7.98e-19 86.3
Msa0621520 MtrunA17_Chr3g0078041 35.659 129 69 1 193 307 49 177 5.94e-18 84.0
Msa0621520 MtrunA17_Chr1g0149131 27.826 115 80 1 437 548 1 115 4.65e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621520 AT3G06250.2 59.533 771 295 9 12 772 1 764 0.0 928
Msa0621520 AT3G06250.1 59.533 771 295 9 12 772 1 764 0.0 928
Msa0621520 AT3G06250.3 59.533 771 295 9 12 772 1 764 0.0 928
Msa0621520 AT5G18960.2 56.456 790 314 11 5 772 7 788 0.0 885
Msa0621520 AT5G18960.1 56.456 790 314 11 5 772 7 788 0.0 885
Msa0621520 AT4G38180.1 36.320 625 348 11 192 768 71 693 4.51e-128 401
Msa0621520 AT4G38180.1 40.870 115 51 2 31 128 63 177 8.51e-14 75.9
Msa0621520 AT2G27110.2 37.997 629 358 14 163 768 16 635 6.59e-123 389
Msa0621520 AT2G27110.1 37.997 629 358 14 163 768 16 635 6.59e-123 389
Msa0621520 AT2G27110.3 41.649 461 252 8 322 768 33 490 4.59e-109 348
Msa0621520 AT4G15090.1 33.171 615 363 11 192 763 49 658 1.32e-105 343
Msa0621520 AT4G15090.5 33.171 615 363 11 192 763 49 658 1.32e-105 343
Msa0621520 AT4G15090.3 33.171 615 363 11 192 763 49 658 1.32e-105 343
Msa0621520 AT4G15090.2 33.171 615 363 11 192 763 49 658 1.32e-105 343
Msa0621520 AT4G15090.4 33.171 615 363 11 192 763 49 658 1.32e-105 343
Msa0621520 AT4G38170.1 40.363 441 251 7 337 769 21 457 1.70e-104 332
Msa0621520 AT1G52520.1 32.846 615 369 14 194 768 85 695 1.77e-100 326
Msa0621520 AT1G52520.1 28.188 149 95 2 40 179 85 230 6.06e-11 66.6
Msa0621520 AT3G22170.2 31.301 607 364 11 192 758 68 661 3.13e-99 326
Msa0621520 AT3G22170.1 31.301 607 364 11 192 758 68 661 3.13e-99 326
Msa0621520 AT1G76320.3 34.124 548 324 10 199 713 2 545 2.07e-89 295
Msa0621520 AT1G76320.1 34.124 548 324 10 199 713 2 545 1.32e-88 295
Msa0621520 AT1G76320.4 34.124 548 324 10 199 713 2 545 1.32e-88 295
Msa0621520 AT1G76320.2 34.124 548 324 10 199 713 2 545 1.33e-88 295
Msa0621520 AT2G32250.5 32.037 540 321 8 197 713 41 557 3.09e-86 289
Msa0621520 AT2G32250.2 32.037 540 321 8 197 713 41 557 7.29e-86 289
Msa0621520 AT2G32250.4 32.037 540 321 8 197 713 41 557 7.29e-86 289
Msa0621520 AT2G32250.6 32.037 540 321 8 197 713 41 557 9.11e-86 289
Msa0621520 AT2G32250.1 32.037 540 321 8 197 713 41 557 9.11e-86 289
Msa0621520 AT2G32250.3 32.037 540 321 8 197 713 41 557 9.11e-86 289
Msa0621520 AT2G32250.8 31.970 538 320 8 199 713 2 516 1.85e-85 287
Msa0621520 AT2G32250.7 31.970 538 320 8 199 713 2 516 2.51e-85 287
Msa0621520 AT1G80010.1 28.617 629 389 14 194 768 95 717 9.95e-85 284
Msa0621520 AT1G80010.1 31.579 114 69 1 27 131 82 195 2.09e-11 68.2
Msa0621520 AT4G19990.5 29.791 574 331 10 182 712 15 559 7.29e-75 258
Msa0621520 AT4G19990.3 29.791 574 331 10 182 712 15 559 9.08e-75 258
Msa0621520 AT4G19990.4 29.791 574 331 10 182 712 15 559 9.08e-75 258
Msa0621520 AT4G19990.7 29.791 574 331 10 182 712 29 573 9.42e-75 258
Msa0621520 AT4G19990.6 29.791 574 331 10 182 712 15 559 1.69e-74 258
Msa0621520 AT4G19990.2 29.791 574 331 10 182 712 15 559 1.69e-74 258
Msa0621520 AT4G19990.1 30.898 479 291 7 251 712 47 502 5.07e-67 235
Msa0621520 AT5G28530.1 28.696 575 336 14 194 707 55 616 2.02e-59 214
Msa0621520 AT5G28530.3 28.348 575 334 14 194 707 55 612 2.95e-59 213
Msa0621520 AT5G28530.2 28.348 575 334 14 194 707 55 612 2.95e-59 213
Msa0621520 AT1G10240.2 27.711 581 347 15 186 709 40 604 8.25e-59 212
Msa0621520 AT1G10240.1 27.711 581 347 15 186 709 40 604 8.25e-59 212
Msa0621520 AT5G28530.4 26.111 540 332 14 194 707 55 553 5.65e-46 174
Msa0621520 AT5G28530.5 25.741 540 330 14 194 707 55 549 8.15e-46 174
Msa0621520 AT3G59470.3 48.039 102 41 1 193 282 69 170 1.65e-23 100
Msa0621520 AT3G59470.3 39.655 116 51 2 25 128 62 170 7.85e-19 87.4
Msa0621520 AT3G59470.1 48.039 102 41 1 193 282 69 170 1.65e-23 100
Msa0621520 AT3G59470.1 39.655 116 51 2 25 128 62 170 7.85e-19 87.4
Msa0621520 AT3G59470.2 48.039 102 41 1 193 282 81 182 2.05e-23 100
Msa0621520 AT3G59470.2 39.655 116 51 2 25 128 74 182 1.14e-18 87.0
Msa0621520 AT3G07500.1 38.532 109 54 1 187 282 26 134 3.29e-19 87.4
Msa0621520 AT3G07500.1 39.806 103 49 1 39 128 32 134 3.09e-16 79.0
Msa0621520 AT4G12850.1 38.732 142 61 3 193 314 10 145 6.79e-19 85.5
Msa0621520 AT4G12850.1 36.923 130 72 1 39 158 10 139 2.03e-18 84.3
Msa0621520 AT2G43280.1 36.232 138 74 1 35 158 17 154 7.15e-19 86.3
Msa0621520 AT2G43280.1 36.364 132 70 2 192 309 20 151 6.69e-16 77.8
Msa0621520 AT4G12850.4 42.982 114 52 2 193 293 10 123 1.72e-18 83.2
Msa0621520 AT4G12850.4 42.574 101 48 1 39 129 10 110 1.85e-18 83.2
Msa0621520 AT4G12850.2 40.909 110 52 2 30 129 4 110 2.56e-18 82.4
Msa0621520 AT4G12850.2 44.000 100 46 1 193 282 10 109 1.39e-17 80.5
Msa0621520 AT2G43280.2 36.441 118 61 1 55 158 2 119 1.81e-15 75.5
Msa0621520 AT2G43280.2 36.522 115 59 2 209 309 2 116 3.41e-13 68.9

Find 175 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGCTCATGCAGCATATT+TGG 0.062384 4_2:-73637501 Msa0621520:CDS
GTAATTGCATGAGCATATTT+TGG 0.147366 4_2:-73637052 Msa0621520:CDS
CTGGGTTCCGTTCTATTTAA+AGG 0.169921 4_2:-73636532 Msa0621520:CDS
TTCTTTGGTGGAAGTTTAAA+TGG 0.183946 4_2:-73636465 Msa0621520:CDS
TGGTGAATGGCGACATATTT+AGG 0.203007 4_2:-73637211 Msa0621520:CDS
TTGCAGTGCTTTAGTTATCT+TGG 0.241216 4_2:-73636255 Msa0621520:CDS
AGGGTTCAGTTAAATGATTC+TGG 0.241282 4_2:-73637815 Msa0621520:CDS
CATGCAGCATATTTGGGTTT+TGG 0.247228 4_2:-73637494 Msa0621520:CDS
CACAAGCAACCTGTGCTTCT+TGG 0.254421 4_2:-73636912 Msa0621520:CDS
ACAAGAGCTCGCCGGTCTTA+TGG 0.267988 4_2:-73636700 Msa0621520:CDS
TGAGAGAGAAATAGTGGAAT+TGG 0.284495 4_2:+73638500 None:intergenic
GATTCCATGCTTTCTTTCAT+AGG 0.296754 4_2:+73636489 None:intergenic
AAAGGGAAGGTAGTAGGATT+TGG 0.298296 4_2:-73638124 Msa0621520:intron
ATAACGCCAAAGGGAGAAAT+AGG 0.315995 4_2:+73637626 None:intergenic
GAAAGCATGGAATCATTCTT+TGG 0.319532 4_2:-73636480 Msa0621520:CDS
TTCATCAATGCTTCAATTCC+AGG 0.329107 4_2:-73638460 Msa0621520:intron
CATGCTCATGCAGCATATTT+GGG 0.329135 4_2:-73637500 Msa0621520:CDS
TGCAGTGCTTTAGTTATCTT+GGG 0.331311 4_2:-73636254 Msa0621520:CDS
GAGAGAGAAATAGTGGAATT+GGG 0.339020 4_2:+73638501 None:intergenic
GGTGAACCTATTTCTCCCTT+TGG 0.344972 4_2:-73637632 Msa0621520:CDS
CAAGAGCTCGCCGGTCTTAT+GGG 0.345700 4_2:-73636699 Msa0621520:CDS
TGGGTTCCGTTCTATTTAAA+GGG 0.346420 4_2:-73636531 Msa0621520:CDS
TATGCCATGGAAGTTGATAA+TGG 0.354230 4_2:-73637074 Msa0621520:CDS
AGGGTCCCTTAGCAGATATT+TGG 0.360553 4_2:-73636214 Msa0621520:CDS
GAATTGAAGCATTGATGAAT+TGG 0.360557 4_2:+73638464 None:intergenic
CTGCATGAGCATGGTAAAAT+TGG 0.364754 4_2:+73637510 None:intergenic
TAAAATTGGTAGGCTTCATT+AGG 0.371960 4_2:+73637524 None:intergenic
ATCGAATGGGATTTAAAGTT+CGG 0.379216 4_2:-73637949 Msa0621520:CDS
AAAAGATCATAATCATGATC+TGG 0.383971 4_2:-73637306 Msa0621520:CDS
AAGGGAAGGTAGTAGGATTT+GGG 0.388720 4_2:-73638123 Msa0621520:intron
ATATAGAAAGAGTGTTTATA+TGG 0.389131 4_2:-73636967 Msa0621520:CDS
TAGTGGAATTGGGGACAAAT+TGG 0.389183 4_2:+73638511 None:intergenic
CGCTTTGTGTGCTCAAAAGA+AGG 0.394545 4_2:-73637416 Msa0621520:CDS
AATGCTGTACTCGTCAAATA+TGG 0.394678 4_2:-73636603 Msa0621520:CDS
CAGTAGAAGAACAGTGTAGA+AGG 0.396970 4_2:-73636320 Msa0621520:CDS
CTGGTTGTCCGGCATTCATA+AGG 0.399148 4_2:-73637835 Msa0621520:CDS
CTTCTACACTGTTCTTCTAC+TGG 0.401126 4_2:+73636321 None:intergenic
GAAAAGCGATGATTGGTTAC+TGG 0.410855 4_2:+73636747 None:intergenic
CTCGCCGGTCTTATGGGTAA+TGG 0.410939 4_2:-73636693 Msa0621520:CDS
CCACAACGAAAAGCGATGAT+TGG 0.412913 4_2:+73636740 None:intergenic
GCCTTACAGCCAACTCTTGA+AGG 0.417533 4_2:+73637383 None:intergenic
CGTCAAATTCATCGACTGTC+TGG 0.424860 4_2:+73636634 None:intergenic
TCGCCGGTCTTATGGGTAAT+GGG 0.426877 4_2:-73636692 Msa0621520:CDS
CGAGGAGGTAAACACTGAAT+TGG 0.426954 4_2:-73637231 Msa0621520:CDS
TGGTAATGAGTAATGCAATT+GGG 0.428684 4_2:-73638063 Msa0621520:CDS
CCCTTCAAGAGTTGGCTGTA+AGG 0.428884 4_2:-73637384 Msa0621520:CDS
AGATACTCATGTAGTCAGTT+TGG 0.430685 4_2:-73637014 Msa0621520:CDS
ATTGATGAATTGGATTGAAA+TGG 0.432866 4_2:+73638474 None:intergenic
GATAACTACCCTGTCCTAAG+AGG 0.442106 4_2:-73637185 Msa0621520:CDS
TTCATTCCGCGTTATGAAAG+AGG 0.443774 4_2:-73636423 Msa0621520:CDS
GCTACAACTGATATCAAGAT+TGG 0.449490 4_2:+73636137 None:intergenic
TGGAATTGAGAGAGAAATAG+TGG 0.454215 4_2:+73638494 None:intergenic
CTGGTAATGAGTAATGCAAT+TGG 0.460733 4_2:-73638064 Msa0621520:CDS
TCATGATCTGGGCCTTGAAA+AGG 0.461115 4_2:-73637294 Msa0621520:CDS
TGTCTAGCCCCTCTTAGGAC+AGG 0.462265 4_2:+73637176 None:intergenic
TCAAGACAAGCAGAAGATAC+AGG 0.463603 4_2:-73637104 Msa0621520:CDS
CATCAGCTCAGCTCAAGAAC+TGG 0.472629 4_2:-73637854 Msa0621520:CDS
TAAGTGTGGAAATGATGATA+TGG 0.473912 4_2:-73636187 Msa0621520:CDS
AAAAGAAGAACTTGAAGTAC+AGG 0.474189 4_2:-73637585 Msa0621520:CDS
AGGATCATGGAGTAGCTCTC+AGG 0.475535 4_2:-73635974 Msa0621520:CDS
GGGTTTAAAATCTATGAAGA+AGG 0.477376 4_2:-73636234 Msa0621520:CDS
AGCGACGTCGTGAAGAAGAA+AGG 0.478827 4_2:-73636395 Msa0621520:CDS
GAGTAATGCAATTGGGGAGG+AGG 0.480640 4_2:-73638056 Msa0621520:CDS
GCATCCTGTTGGTACTGAAC+TGG 0.481228 4_2:-73638083 Msa0621520:CDS
AAAATCACATTAGAAGTGAT+TGG 0.484311 4_2:-73637154 Msa0621520:CDS
TGGTTGTCCGGCATTCATAA+GGG 0.485031 4_2:-73637834 Msa0621520:CDS
AGCCAACTCTTGAAGGGTGC+TGG 0.486059 4_2:+73637390 None:intergenic
AGGATTAAGAATCAACCATC+AGG 0.487093 4_2:-73637353 Msa0621520:CDS
TGCTCCAACGTTGCAGCCGA+AGG 0.487468 4_2:-73637726 Msa0621520:CDS
TCACTTGGTTGTTTCAGACA+TGG 0.489216 4_2:-73636854 Msa0621520:CDS
AACTGAACCCTTATGAATGC+CGG 0.489602 4_2:+73637827 None:intergenic
ACCAACATATGGTTCGATCC+TGG 0.490539 4_2:+73637562 None:intergenic
CTGAACTCTTGACCAACATA+TGG 0.491150 4_2:+73637551 None:intergenic
GTTAAATGATTCTGGAAAGT+GGG 0.491161 4_2:-73637807 Msa0621520:CDS
ATTCATCGACTGTCTGGCTC+TGG 0.491387 4_2:+73636640 None:intergenic
TCTTTGTAGACATGTGTTGA+GGG 0.493205 4_2:-73636088 Msa0621520:CDS
CAGCTCAAGAACTGGTTGTC+CGG 0.494844 4_2:-73637846 Msa0621520:CDS
TGAACTCTATGAGAGGTGTT+TGG 0.495126 4_2:+73636442 None:intergenic
AGGAAGAAAATGCTTGAAGT+AGG 0.495697 4_2:-73637659 Msa0621520:CDS
AGAATCAACCATCAGGAAAG+TGG 0.496214 4_2:-73637346 Msa0621520:CDS
GAAGCCTTCGGCTGCAACGT+TGG 0.496394 4_2:+73637722 None:intergenic
TTCTTTGTAGACATGTGTTG+AGG 0.499747 4_2:-73636089 Msa0621520:CDS
ATATCGTATGGTAATCGAAT+GGG 0.500171 4_2:-73637962 Msa0621520:CDS
CAATTCAGTGTTTACCTCCT+CGG 0.500177 4_2:+73637232 None:intergenic
ATGGATTGAAGGACAATGCT+TGG 0.502036 4_2:-73636584 Msa0621520:CDS
CACTTGGTTGTTTCAGACAT+GGG 0.502099 4_2:-73636853 Msa0621520:CDS
AAAGATCATAATCATGATCT+GGG 0.502626 4_2:-73637305 Msa0621520:CDS
TTCCAGCACCCTTCAAGAGT+TGG 0.503277 4_2:-73637392 Msa0621520:CDS
AGTTAAATGATTCTGGAAAG+TGG 0.505016 4_2:-73637808 Msa0621520:CDS
ACTCGTCAAATATGGATTGA+AGG 0.507225 4_2:-73636595 Msa0621520:CDS
AGGGTGGGAGAAAACTTTGT+TGG 0.507509 4_2:-73635843 Msa0621520:CDS
GGAGAAAACTTTGTTGGCAC+AGG 0.508807 4_2:-73635837 Msa0621520:CDS
AGCTAAAGAGTTTGATGCGA+TGG 0.509137 4_2:-73635951 Msa0621520:CDS
ATTACCAGTTCAGTACCAAC+AGG 0.512740 4_2:+73638079 None:intergenic
TCGATAGCTGAAGAAGCCTT+CGG 0.519394 4_2:+73637710 None:intergenic
ATCAAGATGTTTCAATCCAG+AGG 0.519473 4_2:-73636788 Msa0621520:CDS
ACTCCAACAAAAGTAGCAAA+TGG 0.521017 4_2:+73636942 None:intergenic
GTATGAGAAGCGTGCATCCT+GGG 0.521503 4_2:-73636550 Msa0621520:CDS
ATGGGATTTAAAGTTCGGAT+TGG 0.522097 4_2:-73637944 Msa0621520:CDS
CTACTCCATGATCCTATGTC+GGG 0.522238 4_2:+73635982 None:intergenic
GTTGTGGCAAATTAATGCAA+AGG 0.522725 4_2:-73636724 Msa0621520:CDS
TTTCTTGTGATCCGTACATA+GGG 0.523624 4_2:-73638024 Msa0621520:CDS
CCAAATATGCTGCATGAGCA+TGG 0.525898 4_2:+73637501 None:intergenic
TACCTCCTCGGTCAAAATGT+TGG 0.526158 4_2:+73637244 None:intergenic
TTGGCTGTAAGGCTTATTTG+AGG 0.528311 4_2:-73637373 Msa0621520:CDS
TACGCACCAAATATCTGCTA+AGG 0.529081 4_2:+73636208 None:intergenic
AGATTTGTGTGTTCGAAGGA+GGG 0.530744 4_2:-73637878 Msa0621520:CDS
ATTACCATTATCAACTTCCA+TGG 0.531538 4_2:+73637070 None:intergenic
ATAAAACTTAAGTGCATCAT+CGG 0.532978 4_2:+73637985 None:intergenic
TGTATGAGAAGCGTGCATCC+TGG 0.538953 4_2:-73636551 Msa0621520:CDS
ATTTGGGATAATGATTGTAA+AGG 0.539019 4_2:-73638107 Msa0621520:CDS
TTTATCGATCGAGAACTAAC+GGG 0.539965 4_2:-73637916 Msa0621520:CDS
CTTAAAATTAATAACGCCAA+AGG 0.540683 4_2:+73637616 None:intergenic
GTCTAGCCCCTCTTAGGACA+GGG 0.541407 4_2:+73637177 None:intergenic
TCGGTCAAAATGTTGGAAGC+AGG 0.543963 4_2:+73637251 None:intergenic
TTAAAATTAATAACGCCAAA+GGG 0.546905 4_2:+73637617 None:intergenic
CTTTATCGATCGAGAACTAA+CGG 0.547367 4_2:-73637917 Msa0621520:CDS
TAGAAGATTTGTGTGTTCGA+AGG 0.548021 4_2:-73637882 Msa0621520:CDS
AGAGAGAAATAGTGGAATTG+GGG 0.548230 4_2:+73638502 None:intergenic
AAGATTTGTGTGTTCGAAGG+AGG 0.548897 4_2:-73637879 Msa0621520:CDS
GGGTAAGAGCAATGTCTGGC+CGG 0.549763 4_2:-73636833 Msa0621520:CDS
GGAAGAAAATGCTTGAAGTA+GGG 0.553026 4_2:-73637658 Msa0621520:CDS
GCATCTAAATACATAGATGC+TGG 0.554139 4_2:-73635913 Msa0621520:CDS
CCTCAGCATTTCTCGTCCAT+CGG 0.554803 4_2:+73636016 None:intergenic
ACATGGGTAAGAGCAATGTC+TGG 0.554918 4_2:-73636837 Msa0621520:CDS
TCGACTACCCACTTTCCTGA+TGG 0.556567 4_2:+73637338 None:intergenic
GTAAACACTGAATTGGTGAA+TGG 0.557824 4_2:-73637224 Msa0621520:CDS
GAAGCACAGGTTGCTTGTGG+TGG 0.561382 4_2:+73636916 None:intergenic
TATATCGTATGGTAATCGAA+TGG 0.564167 4_2:-73637963 Msa0621520:CDS
ATAACTACCCTGTCCTAAGA+GGG 0.564605 4_2:-73637184 Msa0621520:CDS
TCGATGAATTTGACGCCTCG+TGG 0.565144 4_2:-73636626 Msa0621520:CDS
GCTACTCCATGATCCTATGT+CGG 0.573087 4_2:+73635981 None:intergenic
AATTCCTATGAAAGAAAGCA+TGG 0.575352 4_2:-73636493 Msa0621520:CDS
AGATATTTGGTGCGTAAGTG+TGG 0.576360 4_2:-73636201 Msa0621520:CDS
GAATCAACCATCAGGAAAGT+GGG 0.580534 4_2:-73637345 Msa0621520:CDS
AGAGCACATCCAAGAAGCAC+AGG 0.580657 4_2:+73636903 None:intergenic
AAGAACTTGAAGTACAGGCC+AGG 0.583073 4_2:-73637580 Msa0621520:CDS
GGTTTAAAATCTATGAAGAA+GGG 0.583657 4_2:-73636233 Msa0621520:CDS
TGTCAGATGTTTGAATATGA+AGG 0.583906 4_2:-73636114 Msa0621520:CDS
CCTTACAGCCAACTCTTGAA+GGG 0.585947 4_2:+73637384 None:intergenic
GCTTTGTGTGCTCAAAAGAA+GGG 0.587200 4_2:-73637415 Msa0621520:CDS
AATGTCCCCTTTAAATAGAA+CGG 0.589456 4_2:+73636525 None:intergenic
CAAGAAGCACAGGTTGCTTG+TGG 0.592747 4_2:+73636913 None:intergenic
ATTGTAAAGGAGCATCCTGT+TGG 0.597584 4_2:-73638094 Msa0621520:CDS
GGGTTCCGTTCTATTTAAAG+GGG 0.600294 4_2:-73636530 Msa0621520:CDS
GCCAGGATCGAACCATATGT+TGG 0.601674 4_2:-73637563 Msa0621520:CDS
AATCTGAAGAGTCTTTCACT+TGG 0.601960 4_2:-73636869 Msa0621520:CDS
CTTTAAATAGAACGGAACCC+AGG 0.602743 4_2:+73636533 None:intergenic
CTGAAGTCACTCGCAAGCCA+AGG 0.602865 4_2:-73637679 Msa0621520:CDS
CTATTACTGACAGAGGCTGC+CGG 0.603031 4_2:+73636814 None:intergenic
GGTAATGAGTAATGCAATTG+GGG 0.604332 4_2:-73638062 Msa0621520:CDS
ATGAGCATGGTAAAATTGGT+AGG 0.604922 4_2:+73637514 None:intergenic
AGCATGGAATCATTCTTTGG+TGG 0.604989 4_2:-73636477 Msa0621520:CDS
TCGTGAAGAAGAAAGGAAAG+AGG 0.606355 4_2:-73636388 Msa0621520:CDS
CTCGCTACATCTTACACCGA+TGG 0.607094 4_2:-73636032 Msa0621520:CDS
GAACCCATTACCCATAAGAC+CGG 0.608640 4_2:+73636689 None:intergenic
GCAAAGGAACAAGAGCTCGC+CGG 0.611525 4_2:-73636708 Msa0621520:CDS
AAAATGCTTGAAGTAGGGAA+TGG 0.612218 4_2:-73637653 Msa0621520:CDS
ACGCACCAAATATCTGCTAA+GGG 0.623887 4_2:+73636209 None:intergenic
CGCGGAATGAACTCTATGAG+AGG 0.627384 4_2:+73636435 None:intergenic
CGGTGTAAGATGTAGCGAGA+AGG 0.633470 4_2:+73636036 None:intergenic
TCAAGATGTTTCAATCCAGA+GGG 0.646236 4_2:-73636787 Msa0621520:CDS
AATGAGTAATGCAATTGGGG+AGG 0.656176 4_2:-73638059 Msa0621520:CDS
AATTGGAGAGGTGTAAAGCA+CGG 0.656686 4_2:+73638528 None:intergenic
TCGATCGAGAACTAACGGGT+CGG 0.658018 4_2:-73637912 Msa0621520:CDS
TGGACGAGAAATGCTGAGGA+TGG 0.668666 4_2:-73636012 Msa0621520:CDS
ACCCTCTCGTAAAATCTCGT+AGG 0.677797 4_2:+73635861 None:intergenic
TGATCAGCTATTACTGACAG+AGG 0.686515 4_2:+73636807 None:intergenic
CCGATGGACGAGAAATGCTG+AGG 0.692169 4_2:-73636016 Msa0621520:CDS
GAATTGGGGACAAATTGGAG+AGG 0.694086 4_2:+73638516 None:intergenic
TAACTACCCTGTCCTAAGAG+GGG 0.695809 4_2:-73637183 Msa0621520:CDS
TCAAGACCTCTTTCATAACG+CGG 0.715495 4_2:+73636417 None:intergenic
GACGAGTACAGCATTCCACG+AGG 0.753419 4_2:+73636611 None:intergenic
TGGAGAGGTGTAAAGCACGG+AGG 0.774334 4_2:+73638531 None:intergenic
TATATGATGTATCTACAACG+AGG 0.828520 4_2:+73636985 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAATGAAAAATTTACC+TGG + chr4_2:73635923-73635942 None:intergenic 20.0%
!! ACTAAGAAATCGAAATAATT+GGG + chr4_2:73635998-73636017 None:intergenic 20.0%
!! AACTAAGAAATCGAAATAAT+TGG + chr4_2:73635999-73636018 None:intergenic 20.0%
!!! CTTAAGTTTTATATATCGTA+TGG - chr4_2:73636388-73636407 Msa0621520:CDS 20.0%
!! TTAAAATTAATAACGCCAAA+GGG + chr4_2:73636748-73636767 None:intergenic 20.0%
!!! ATATAGAAAGAGTGTTTATA+TGG - chr4_2:73637395-73637414 Msa0621520:CDS 20.0%
! ATTGATGAATTGGATTGAAA+TGG + chr4_2:73635891-73635910 None:intergenic 25.0%
! AGGAGGTAAATTAATTACTA+CGG + chr4_2:73636060-73636079 None:intergenic 25.0%
! ATAAAATTGTCATCATCTGA+AGG + chr4_2:73636080-73636099 None:intergenic 25.0%
!!! CTTTTGTTTGCTTTGAAAAA+GGG - chr4_2:73636221-73636240 Msa0621520:CDS 25.0%
!! ATTTGGGATAATGATTGTAA+AGG - chr4_2:73636255-73636274 Msa0621520:CDS 25.0%
! ATAAAACTTAAGTGCATCAT+CGG + chr4_2:73636380-73636399 None:intergenic 25.0%
! CTTAAAATTAATAACGCCAA+AGG + chr4_2:73636749-73636768 None:intergenic 25.0%
!!! TTGTTTCGTTCTAAAAATGA+CGG - chr4_2:73636907-73636926 Msa0621520:CDS 25.0%
!!! GATTATGATCTTTTTCAAGA+CGG + chr4_2:73637050-73637069 None:intergenic 25.0%
! AAAAGATCATAATCATGATC+TGG - chr4_2:73637056-73637075 Msa0621520:CDS 25.0%
! AAAGATCATAATCATGATCT+GGG - chr4_2:73637057-73637076 Msa0621520:CDS 25.0%
! AAAATCACATTAGAAGTGAT+TGG - chr4_2:73637208-73637227 Msa0621520:CDS 25.0%
!! TAATTGCATGAGCATATTTT+GGG - chr4_2:73637311-73637330 Msa0621520:CDS 25.0%
!! GGTTTAAAATCTATGAAGAA+GGG - chr4_2:73638129-73638148 Msa0621520:intron 25.0%
!! GAATTGAAGCATTGATGAAT+TGG + chr4_2:73635901-73635920 None:intergenic 30.0%
GGAATGGATCGAAATAATTA+GGG + chr4_2:73635948-73635967 None:intergenic 30.0%
!!! GCTTTTGTTTGCTTTGAAAA+AGG - chr4_2:73636220-73636239 Msa0621520:CDS 30.0%
! TGGTAATGAGTAATGCAATT+GGG - chr4_2:73636299-73636318 Msa0621520:CDS 30.0%
AAAAACTCTAACCCTATGTA+CGG + chr4_2:73636352-73636371 None:intergenic 30.0%
TATATCGTATGGTAATCGAA+TGG - chr4_2:73636399-73636418 Msa0621520:CDS 30.0%
ATATCGTATGGTAATCGAAT+GGG - chr4_2:73636400-73636419 Msa0621520:CDS 30.0%
! ATCGAATGGGATTTAAAGTT+CGG - chr4_2:73636413-73636432 Msa0621520:CDS 30.0%
! AGTTAAATGATTCTGGAAAG+TGG - chr4_2:73636554-73636573 Msa0621520:CDS 30.0%
! GTTAAATGATTCTGGAAAGT+GGG - chr4_2:73636555-73636574 Msa0621520:CDS 30.0%
! GGTTGTTGATCATTTTCATA+AGG - chr4_2:73636576-73636595 Msa0621520:CDS 30.0%
AAAAGAAGAACTTGAAGTAC+AGG - chr4_2:73636777-73636796 Msa0621520:CDS 30.0%
TAAAATTGGTAGGCTTCATT+AGG + chr4_2:73636841-73636860 None:intergenic 30.0%
ATTACCATTATCAACTTCCA+TGG + chr4_2:73637295-73637314 None:intergenic 30.0%
! GTAATTGCATGAGCATATTT+TGG - chr4_2:73637310-73637329 Msa0621520:CDS 30.0%
TATATGATGTATCTACAACG+AGG + chr4_2:73637380-73637399 None:intergenic 30.0%
AATGTCCCCTTTAAATAGAA+CGG + chr4_2:73637840-73637859 None:intergenic 30.0%
AATTCCTATGAAAGAAAGCA+TGG - chr4_2:73637869-73637888 Msa0621520:CDS 30.0%
TTCTTTGGTGGAAGTTTAAA+TGG - chr4_2:73637897-73637916 Msa0621520:CDS 30.0%
! GGGTTTAAAATCTATGAAGA+AGG - chr4_2:73638128-73638147 Msa0621520:intron 30.0%
TAAGTGTGGAAATGATGATA+TGG - chr4_2:73638175-73638194 Msa0621520:intron 30.0%
TGTCAGATGTTTGAATATGA+AGG - chr4_2:73638248-73638267 Msa0621520:intron 30.0%
!! GTTGAGGGTTTTTCAAATAT+TGG - chr4_2:73638289-73638308 Msa0621520:intron 30.0%
! AGAGAGAAATAGTGGAATTG+GGG + chr4_2:73635863-73635882 None:intergenic 35.0%
! GAGAGAGAAATAGTGGAATT+GGG + chr4_2:73635864-73635883 None:intergenic 35.0%
! TGAGAGAGAAATAGTGGAAT+TGG + chr4_2:73635865-73635884 None:intergenic 35.0%
TGGAATTGAGAGAGAAATAG+TGG + chr4_2:73635871-73635890 None:intergenic 35.0%
TTCATCAATGCTTCAATTCC+AGG - chr4_2:73635902-73635921 Msa0621520:CDS 35.0%
GGGAATGGATCGAAATAATT+AGG + chr4_2:73635949-73635968 None:intergenic 35.0%
CAATAAAAAGTCAAAGCAGC+GGG + chr4_2:73635969-73635988 None:intergenic 35.0%
GCAATAAAAAGTCAAAGCAG+CGG + chr4_2:73635970-73635989 None:intergenic 35.0%
AAATTGTCATCATCTGAAGG+AGG + chr4_2:73636077-73636096 None:intergenic 35.0%
! AGTGCTTTTCTCTTAATGTG+TGG - chr4_2:73636192-73636211 Msa0621520:CDS 35.0%
!! TGTTTGCTTTGAAAAAGGGA+AGG - chr4_2:73636225-73636244 Msa0621520:CDS 35.0%
TTTGAAAAAGGGAAGGTAGT+AGG - chr4_2:73636232-73636251 Msa0621520:CDS 35.0%
! CTGGTAATGAGTAATGCAAT+TGG - chr4_2:73636298-73636317 Msa0621520:CDS 35.0%
GGTAATGAGTAATGCAATTG+GGG - chr4_2:73636300-73636319 Msa0621520:CDS 35.0%
! TTTTCTTGTGATCCGTACAT+AGG - chr4_2:73636337-73636356 Msa0621520:CDS 35.0%
TTTCTTGTGATCCGTACATA+GGG - chr4_2:73636338-73636357 Msa0621520:CDS 35.0%
! ATGGGATTTAAAGTTCGGAT+TGG - chr4_2:73636418-73636437 Msa0621520:CDS 35.0%
CTTTATCGATCGAGAACTAA+CGG - chr4_2:73636445-73636464 Msa0621520:CDS 35.0%
TTTATCGATCGAGAACTAAC+GGG - chr4_2:73636446-73636465 Msa0621520:CDS 35.0%
TAGAAGATTTGTGTGTTCGA+AGG - chr4_2:73636480-73636499 Msa0621520:CDS 35.0%
AGGGTTCAGTTAAATGATTC+TGG - chr4_2:73636547-73636566 Msa0621520:CDS 35.0%
!! CACTTTCATTTTCAAGAGTG+TGG + chr4_2:73636609-73636628 None:intergenic 35.0%
! ACTTCAAGCATTTTCTTCCT+TGG + chr4_2:73636703-73636722 None:intergenic 35.0%
AGGAAGAAAATGCTTGAAGT+AGG - chr4_2:73636703-73636722 Msa0621520:CDS 35.0%
GGAAGAAAATGCTTGAAGTA+GGG - chr4_2:73636704-73636723 Msa0621520:CDS 35.0%
AAAATGCTTGAAGTAGGGAA+TGG - chr4_2:73636709-73636728 Msa0621520:CDS 35.0%
ATGAGCATGGTAAAATTGGT+AGG + chr4_2:73636851-73636870 None:intergenic 35.0%
AGGATTAAGAATCAACCATC+AGG - chr4_2:73637009-73637028 Msa0621520:CDS 35.0%
! TTGTCTTACGTTCCTTTTCA+AGG + chr4_2:73637083-73637102 None:intergenic 35.0%
GTAAACACTGAATTGGTGAA+TGG - chr4_2:73637138-73637157 Msa0621520:CDS 35.0%
! TACAGGATTCTTTTATGCCA+TGG - chr4_2:73637275-73637294 Msa0621520:CDS 35.0%
!! TATGCCATGGAAGTTGATAA+TGG - chr4_2:73637288-73637307 Msa0621520:CDS 35.0%
AGATACTCATGTAGTCAGTT+TGG - chr4_2:73637348-73637367 Msa0621520:CDS 35.0%
!!! GTACCATTTGCTACTTTTGT+TGG - chr4_2:73637417-73637436 Msa0621520:CDS 35.0%
ACTCCAACAAAAGTAGCAAA+TGG + chr4_2:73637423-73637442 None:intergenic 35.0%
AATCTGAAGAGTCTTTCACT+TGG - chr4_2:73637493-73637512 Msa0621520:CDS 35.0%
ATCAAGATGTTTCAATCCAG+AGG - chr4_2:73637574-73637593 Msa0621520:CDS 35.0%
TCAAGATGTTTCAATCCAGA+GGG - chr4_2:73637575-73637594 Msa0621520:CDS 35.0%
GTTGTGGCAAATTAATGCAA+AGG - chr4_2:73637638-73637657 Msa0621520:CDS 35.0%
AATGCTGTACTCGTCAAATA+TGG - chr4_2:73637759-73637778 Msa0621520:CDS 35.0%
! ACTCGTCAAATATGGATTGA+AGG - chr4_2:73637767-73637786 Msa0621520:CDS 35.0%
TGGGTTCCGTTCTATTTAAA+GGG - chr4_2:73637831-73637850 Msa0621520:CDS 35.0%
GATTCCATGCTTTCTTTCAT+AGG + chr4_2:73637876-73637895 None:intergenic 35.0%
! GAAAGCATGGAATCATTCTT+TGG - chr4_2:73637882-73637901 Msa0621520:CDS 35.0%
TTGCAGTGCTTTAGTTATCT+TGG - chr4_2:73638107-73638126 Msa0621520:CDS 35.0%
TGCAGTGCTTTAGTTATCTT+GGG - chr4_2:73638108-73638127 Msa0621520:CDS 35.0%
GCTACAACTGATATCAAGAT+TGG + chr4_2:73638228-73638247 None:intergenic 35.0%
TTCTTTGTAGACATGTGTTG+AGG - chr4_2:73638273-73638292 Msa0621520:intron 35.0%
TCTTTGTAGACATGTGTTGA+GGG - chr4_2:73638274-73638293 Msa0621520:intron 35.0%
GCATCTAAATACATAGATGC+TGG - chr4_2:73638449-73638468 Msa0621520:intron 35.0%
! AATTGGAGAGGTGTAAAGCA+CGG + chr4_2:73635837-73635856 None:intergenic 40.0%
TAGTGGAATTGGGGACAAAT+TGG + chr4_2:73635854-73635873 None:intergenic 40.0%
TGCATCATAACTTTCCATGC+AGG - chr4_2:73636165-73636184 Msa0621520:CDS 40.0%
!! GAAAAGCACTTATTCCTGCA+TGG + chr4_2:73636182-73636201 None:intergenic 40.0%
AAAGGGAAGGTAGTAGGATT+TGG - chr4_2:73636238-73636257 Msa0621520:CDS 40.0%
AAGGGAAGGTAGTAGGATTT+GGG - chr4_2:73636239-73636258 Msa0621520:CDS 40.0%
!! ATTGTAAAGGAGCATCCTGT+TGG - chr4_2:73636268-73636287 Msa0621520:CDS 40.0%
ATTACCAGTTCAGTACCAAC+AGG + chr4_2:73636286-73636305 None:intergenic 40.0%
AATGAGTAATGCAATTGGGG+AGG - chr4_2:73636303-73636322 Msa0621520:CDS 40.0%
AAGATTTGTGTGTTCGAAGG+AGG - chr4_2:73636483-73636502 Msa0621520:CDS 40.0%
AGATTTGTGTGTTCGAAGGA+GGG - chr4_2:73636484-73636503 Msa0621520:CDS 40.0%
AACTGAACCCTTATGAATGC+CGG + chr4_2:73636538-73636557 None:intergenic 40.0%
ATAACGCCAAAGGGAGAAAT+AGG + chr4_2:73636739-73636758 None:intergenic 40.0%
CTGAACTCTTGACCAACATA+TGG + chr4_2:73636814-73636833 None:intergenic 40.0%
CTGCATGAGCATGGTAAAAT+TGG + chr4_2:73636855-73636874 None:intergenic 40.0%
CATGCTCATGCAGCATATTT+GGG - chr4_2:73636862-73636881 Msa0621520:CDS 40.0%
!! CATGCAGCATATTTGGGTTT+TGG - chr4_2:73636868-73636887 Msa0621520:CDS 40.0%
!!! TTGGGTTTTGGAATTCGAGT+CGG - chr4_2:73636880-73636899 Msa0621520:CDS 40.0%
GCTTTGTGTGCTCAAAAGAA+GGG - chr4_2:73636947-73636966 Msa0621520:CDS 40.0%
TTGGCTGTAAGGCTTATTTG+AGG - chr4_2:73636989-73637008 Msa0621520:CDS 40.0%
AGAATCAACCATCAGGAAAG+TGG - chr4_2:73637016-73637035 Msa0621520:CDS 40.0%
GAATCAACCATCAGGAAAGT+GGG - chr4_2:73637017-73637036 Msa0621520:CDS 40.0%
CAATTCAGTGTTTACCTCCT+CGG + chr4_2:73637133-73637152 None:intergenic 40.0%
TGGTGAATGGCGACATATTT+AGG - chr4_2:73637151-73637170 Msa0621520:CDS 40.0%
ATAACTACCCTGTCCTAAGA+GGG - chr4_2:73637178-73637197 Msa0621520:CDS 40.0%
TCAAGACAAGCAGAAGATAC+AGG - chr4_2:73637258-73637277 Msa0621520:CDS 40.0%
!! ATGAGCATATTTTGGGCTGA+CGG - chr4_2:73637318-73637337 Msa0621520:CDS 40.0%
! TCACTTGGTTGTTTCAGACA+TGG - chr4_2:73637508-73637527 Msa0621520:CDS 40.0%
! CACTTGGTTGTTTCAGACAT+GGG - chr4_2:73637509-73637528 Msa0621520:CDS 40.0%
TGATCAGCTATTACTGACAG+AGG + chr4_2:73637558-73637577 None:intergenic 40.0%
! GAAAAGCGATGATTGGTTAC+TGG + chr4_2:73637618-73637637 None:intergenic 40.0%
! ATGGATTGAAGGACAATGCT+TGG - chr4_2:73637778-73637797 Msa0621520:CDS 40.0%
CTTTAAATAGAACGGAACCC+AGG + chr4_2:73637832-73637851 None:intergenic 40.0%
CTGGGTTCCGTTCTATTTAA+AGG - chr4_2:73637830-73637849 Msa0621520:CDS 40.0%
GGGTTCCGTTCTATTTAAAG+GGG - chr4_2:73637832-73637851 Msa0621520:CDS 40.0%
! TTAAAGGGGACATTTTCTGC+TGG - chr4_2:73637846-73637865 Msa0621520:CDS 40.0%
AGCATGGAATCATTCTTTGG+TGG - chr4_2:73637885-73637904 Msa0621520:CDS 40.0%
!! TGAACTCTATGAGAGGTGTT+TGG + chr4_2:73637923-73637942 None:intergenic 40.0%
TTCATTCCGCGTTATGAAAG+AGG - chr4_2:73637939-73637958 Msa0621520:CDS 40.0%
TCAAGACCTCTTTCATAACG+CGG + chr4_2:73637948-73637967 None:intergenic 40.0%
TCGTGAAGAAGAAAGGAAAG+AGG - chr4_2:73637974-73637993 Msa0621520:CDS 40.0%
CTTCTACACTGTTCTTCTAC+TGG + chr4_2:73638044-73638063 None:intergenic 40.0%
CAGTAGAAGAACAGTGTAGA+AGG - chr4_2:73638042-73638061 Msa0621520:CDS 40.0%
ACGCACCAAATATCTGCTAA+GGG + chr4_2:73638156-73638175 None:intergenic 40.0%
TACGCACCAAATATCTGCTA+AGG + chr4_2:73638157-73638176 None:intergenic 40.0%
!! AGATATTTGGTGCGTAAGTG+TGG - chr4_2:73638161-73638180 Msa0621520:intron 40.0%
! TTTTTCCCGACATAGGATCA+TGG - chr4_2:73638375-73638394 Msa0621520:intron 40.0%
AGCTAAAGAGTTTGATGCGA+TGG - chr4_2:73638411-73638430 Msa0621520:intron 40.0%
GAATTGGGGACAAATTGGAG+AGG + chr4_2:73635849-73635868 None:intergenic 45.0%
AAAAGTCAAAGCAGCGGGAA+TGG + chr4_2:73635964-73635983 None:intergenic 45.0%
TGGTTGTCCGGCATTCATAA+GGG - chr4_2:73636528-73636547 Msa0621520:CDS 45.0%
TCGATAGCTGAAGAAGCCTT+CGG + chr4_2:73636655-73636674 None:intergenic 45.0%
! GGTGAACCTATTTCTCCCTT+TGG - chr4_2:73636730-73636749 Msa0621520:CDS 45.0%
AAGAACTTGAAGTACAGGCC+AGG - chr4_2:73636782-73636801 Msa0621520:CDS 45.0%
ACCAACATATGGTTCGATCC+TGG + chr4_2:73636803-73636822 None:intergenic 45.0%
CCAAATATGCTGCATGAGCA+TGG + chr4_2:73636864-73636883 None:intergenic 45.0%
CCATGCTCATGCAGCATATT+TGG - chr4_2:73636861-73636880 Msa0621520:CDS 45.0%
CGCTTTGTGTGCTCAAAAGA+AGG - chr4_2:73636946-73636965 Msa0621520:CDS 45.0%
CCTTACAGCCAACTCTTGAA+GGG + chr4_2:73636981-73637000 None:intergenic 45.0%
TCATGATCTGGGCCTTGAAA+AGG - chr4_2:73637068-73637087 Msa0621520:CDS 45.0%
TCGGTCAAAATGTTGGAAGC+AGG + chr4_2:73637114-73637133 None:intergenic 45.0%
!! TGCTTCCAACATTTTGACCG+AGG - chr4_2:73637113-73637132 Msa0621520:CDS 45.0%
!! TTCCAACATTTTGACCGAGG+AGG - chr4_2:73637116-73637135 Msa0621520:CDS 45.0%
TACCTCCTCGGTCAAAATGT+TGG + chr4_2:73637121-73637140 None:intergenic 45.0%
CGAGGAGGTAAACACTGAAT+TGG - chr4_2:73637131-73637150 Msa0621520:CDS 45.0%
GATAACTACCCTGTCCTAAG+AGG - chr4_2:73637177-73637196 Msa0621520:CDS 45.0%
TAACTACCCTGTCCTAAGAG+GGG - chr4_2:73637179-73637198 Msa0621520:CDS 45.0%
! GATTTTGTCTAGCCCCTCTT+AGG + chr4_2:73637194-73637213 None:intergenic 45.0%
ACATGGGTAAGAGCAATGTC+TGG - chr4_2:73637525-73637544 Msa0621520:CDS 45.0%
!!! AGACTTTTGCTATTGCCCTC+TGG + chr4_2:73637593-73637612 None:intergenic 45.0%
CCACAACGAAAAGCGATGAT+TGG + chr4_2:73637625-73637644 None:intergenic 45.0%
!! CCAATCATCGCTTTTCGTTG+TGG - chr4_2:73637622-73637641 Msa0621520:CDS 45.0%
GAACCCATTACCCATAAGAC+CGG + chr4_2:73637676-73637695 None:intergenic 45.0%
! CGTCAAATTCATCGACTGTC+TGG + chr4_2:73637731-73637750 None:intergenic 45.0%
AGGGTCCCTTAGCAGATATT+TGG - chr4_2:73638148-73638167 Msa0621520:intron 45.0%
! GATGGTGTTTTTCCCGACAT+AGG - chr4_2:73638368-73638387 Msa0621520:intron 45.0%
CTACTCCATGATCCTATGTC+GGG + chr4_2:73638383-73638402 None:intergenic 45.0%
GCTACTCCATGATCCTATGT+CGG + chr4_2:73638384-73638403 None:intergenic 45.0%
! TGCCTACGAGATTTTACGAG+AGG - chr4_2:73638499-73638518 Msa0621520:five_prime_UTR 45.0%
! GCCTACGAGATTTTACGAGA+GGG - chr4_2:73638500-73638519 Msa0621520:five_prime_UTR 45.0%
ACCCTCTCGTAAAATCTCGT+AGG + chr4_2:73638504-73638523 None:intergenic 45.0%
! TACGAGATTTTACGAGAGGG+TGG - chr4_2:73638503-73638522 Msa0621520:five_prime_UTR 45.0%
! ACGAGATTTTACGAGAGGGT+GGG - chr4_2:73638504-73638523 Msa0621520:five_prime_UTR 45.0%
AGGGTGGGAGAAAACTTTGT+TGG - chr4_2:73638519-73638538 Msa0621520:five_prime_UTR 45.0%
GGAGAAAACTTTGTTGGCAC+AGG - chr4_2:73638525-73638544 Msa0621520:five_prime_UTR 45.0%
! GCATCCTGTTGGTACTGAAC+TGG - chr4_2:73636279-73636298 Msa0621520:CDS 50.0%
GAGTAATGCAATTGGGGAGG+AGG - chr4_2:73636306-73636325 Msa0621520:CDS 50.0%
! TCGATCGAGAACTAACGGGT+CGG - chr4_2:73636450-73636469 Msa0621520:CDS 50.0%
CATCAGCTCAGCTCAAGAAC+TGG - chr4_2:73636508-73636527 Msa0621520:CDS 50.0%
CAGCTCAAGAACTGGTTGTC+CGG - chr4_2:73636516-73636535 Msa0621520:CDS 50.0%
CTGGTTGTCCGGCATTCATA+AGG - chr4_2:73636527-73636546 Msa0621520:CDS 50.0%
GCCAGGATCGAACCATATGT+TGG - chr4_2:73636799-73636818 Msa0621520:CDS 50.0%
TTCCAGCACCCTTCAAGAGT+TGG - chr4_2:73636970-73636989 Msa0621520:CDS 50.0%
CCCTTCAAGAGTTGGCTGTA+AGG - chr4_2:73636978-73636997 Msa0621520:CDS 50.0%
GCCTTACAGCCAACTCTTGA+AGG + chr4_2:73636982-73637001 None:intergenic 50.0%
TCGACTACCCACTTTCCTGA+TGG + chr4_2:73637027-73637046 None:intergenic 50.0%
! CAAGAAGCACAGGTTGCTTG+TGG + chr4_2:73637452-73637471 None:intergenic 50.0%
CACAAGCAACCTGTGCTTCT+TGG - chr4_2:73637450-73637469 Msa0621520:CDS 50.0%
! AGAGCACATCCAAGAAGCAC+AGG + chr4_2:73637462-73637481 None:intergenic 50.0%
CTATTACTGACAGAGGCTGC+CGG + chr4_2:73637551-73637570 None:intergenic 50.0%
! TCGCCGGTCTTATGGGTAAT+GGG - chr4_2:73637670-73637689 Msa0621520:CDS 50.0%
! ATTCATCGACTGTCTGGCTC+TGG + chr4_2:73637725-73637744 None:intergenic 50.0%
TCGATGAATTTGACGCCTCG+TGG - chr4_2:73637736-73637755 Msa0621520:CDS 50.0%
! TGTATGAGAAGCGTGCATCC+TGG - chr4_2:73637811-73637830 Msa0621520:CDS 50.0%
! GTATGAGAAGCGTGCATCCT+GGG - chr4_2:73637812-73637831 Msa0621520:CDS 50.0%
CGCGGAATGAACTCTATGAG+AGG + chr4_2:73637930-73637949 None:intergenic 50.0%
AGCGACGTCGTGAAGAAGAA+AGG - chr4_2:73637967-73637986 Msa0621520:CDS 50.0%
CGGTGTAAGATGTAGCGAGA+AGG + chr4_2:73638329-73638348 None:intergenic 50.0%
CTCGCTACATCTTACACCGA+TGG - chr4_2:73638330-73638349 Msa0621520:intron 50.0%
CCTCAGCATTTCTCGTCCAT+CGG + chr4_2:73638349-73638368 None:intergenic 50.0%
TGGACGAGAAATGCTGAGGA+TGG - chr4_2:73638350-73638369 Msa0621520:intron 50.0%
AGGATCATGGAGTAGCTCTC+AGG - chr4_2:73638388-73638407 Msa0621520:intron 50.0%
CTGAAGTCACTCGCAAGCCA+AGG - chr4_2:73636683-73636702 Msa0621520:CDS 55.0%
! AGCCAACTCTTGAAGGGTGC+TGG + chr4_2:73636975-73636994 None:intergenic 55.0%
GTCTAGCCCCTCTTAGGACA+GGG + chr4_2:73637188-73637207 None:intergenic 55.0%
TGTCTAGCCCCTCTTAGGAC+AGG + chr4_2:73637189-73637208 None:intergenic 55.0%
GAAGCACAGGTTGCTTGTGG+TGG + chr4_2:73637449-73637468 None:intergenic 55.0%
GGGTAAGAGCAATGTCTGGC+CGG - chr4_2:73637529-73637548 Msa0621520:CDS 55.0%
GCAAAGGAACAAGAGCTCGC+CGG - chr4_2:73637654-73637673 Msa0621520:CDS 55.0%
ACAAGAGCTCGCCGGTCTTA+TGG - chr4_2:73637662-73637681 Msa0621520:CDS 55.0%
! CAAGAGCTCGCCGGTCTTAT+GGG - chr4_2:73637663-73637682 Msa0621520:CDS 55.0%
! CTCGCCGGTCTTATGGGTAA+TGG - chr4_2:73637669-73637688 Msa0621520:CDS 55.0%
GACGAGTACAGCATTCCACG+AGG + chr4_2:73637754-73637773 None:intergenic 55.0%
CCGATGGACGAGAAATGCTG+AGG - chr4_2:73638346-73638365 Msa0621520:intron 55.0%
TGCTCCAACGTTGCAGCCGA+AGG - chr4_2:73636636-73636655 Msa0621520:CDS 60.0%
GAAGCCTTCGGCTGCAACGT+TGG + chr4_2:73636643-73636662 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 73635834 73638550 73635834 ID=Msa0621520;Name=Msa0621520
chr4_2 mRNA 73635834 73638550 73635834 ID=Msa0621520-mRNA-1;Parent=Msa0621520;Name=Msa0621520-mRNA-1;_AED=0.08;_eAED=0.90;_QI=75|0|0.5|1|0|0|2|0|772
chr4_2 exon 73635834 73638137 73635834 ID=Msa0621520-mRNA-1:exon:22138;Parent=Msa0621520-mRNA-1
chr4_2 exon 73638461 73638550 73638461 ID=Msa0621520-mRNA-1:exon:22137;Parent=Msa0621520-mRNA-1
chr4_2 five_prime_UTR 73638476 73638550 73638476 ID=Msa0621520-mRNA-1:five_prime_utr;Parent=Msa0621520-mRNA-1
chr4_2 CDS 73638461 73638475 73638461 ID=Msa0621520-mRNA-1:cds;Parent=Msa0621520-mRNA-1
chr4_2 CDS 73635834 73638137 73635834 ID=Msa0621520-mRNA-1:cds;Parent=Msa0621520-mRNA-1
Gene Sequence

>Msa0621520

ATGCTTCAATTCCAGGTAGTAGGATTTGGGATAATGATTGTAAAGGAGCATCCTGTTGGTACTGAACTGGTAATGAGTAATGCAATTGGGGAGGAGGAAATTGATTTTTCTTGTGATCCGTACATAGGGTTAGAGTTTTTTAATGCCGATGATGCACTTAAGTTTTATATATCGTATGGTAATCGAATGGGATTTAAAGTTCGGATTGGTCAACTTTATCGATCGAGAACTAACGGGTCGGTTTCTTCTAGAAGATTTGTGTGTTCGAAGGAGGGACATCAGCTCAGCTCAAGAACTGGTTGTCCGGCATTCATAAGGGTTCAGTTAAATGATTCTGGAAAGTGGGTTGTTGATCATTTTCATAAGGATCATAACCACACTCTTGAAAATGAAAGTGAAAGTTTTGCTCCAACGTTGCAGCCGAAGGCTTCTTCAGCTATCGAGTCTTCTACTGAAGTCACTCGCAAGCCAAGGAAGAAAATGCTTGAAGTAGGGAATGGTGAACCTATTTCTCCCTTTGGCGTTATTAATTTTAAGCGTATGAGAAAAGAAGAACTTGAAGTACAGGCCAGGATCGAACCATATGTTGGTCAAGAGTTCAGTACTCCTAATGAAGCCTACCAATTTTACCATGCTCATGCAGCATATTTGGGTTTTGGAATTCGAGTCGGTCAATTGTTTCGTTCTAAAAATGACGGATCAATTACATCTCGACGCTTTGTGTGCTCAAAAGAAGGGTTCCAGCACCCTTCAAGAGTTGGCTGTAAGGCTTATTTGAGGATTAAGAATCAACCATCAGGAAAGTGGGTAGTCGACCGTCTTGAAAAAGATCATAATCATGATCTGGGCCTTGAAAAGGAACGTAAGACAAAAAGTCTTCCTGCTTCCAACATTTTGACCGAGGAGGTAAACACTGAATTGGTGAATGGCGACATATTTAGGATAGATAACTACCCTGTCCTAAGAGGGGCTAGACAAAATCACATTAGAAGTGATTGGTACAACATGCTTTTAGAGTATTTTCAGTCAAGACAAGCAGAAGATACAGGATTCTTTTATGCCATGGAAGTTGATAATGGTAATTGCATGAGCATATTTTGGGCTGACGGAAGATCTAGATACTCATGTAGTCAGTTTGGCGATGTCCTCGTTGTAGATACATCATATAGAAAGAGTGTTTATATGGTACCATTTGCTACTTTTGTTGGAGTTAACCACCACAAGCAACCTGTGCTTCTTGGATGTGCTCTAATTGCTGATGAATCTGAAGAGTCTTTCACTTGGTTGTTTCAGACATGGGTAAGAGCAATGTCTGGCCGGCAGCCTCTGTCAGTAATAGCTGATCAAGATGTTTCAATCCAGAGGGCAATAGCAAAAGTCTTTCCAGTAACCAATCATCGCTTTTCGTTGTGGCAAATTAATGCAAAGGAACAAGAGCTCGCCGGTCTTATGGGTAATGGGTTCACTAAAGATTATGAAAATTGTGTTTACCAGAGCCAGACAGTCGATGAATTTGACGCCTCGTGGAATGCTGTACTCGTCAAATATGGATTGAAGGACAATGCTTGGCTAAAAGAAATGTATGAGAAGCGTGCATCCTGGGTTCCGTTCTATTTAAAGGGGACATTTTCTGCTGGAATTCCTATGAAAGAAAGCATGGAATCATTCTTTGGTGGAAGTTTAAATGGCCAAACACCTCTCATAGAGTTCATTCCGCGTTATGAAAGAGGTCTTGAGCGACGTCGTGAAGAAGAAAGGAAAGAGGATTTTAATACTTCTAATTTTCAAACTTTTTTGCAAACGAAAGAACCAGTAGAAGAACAGTGTAGAAGGCTTTACACTCTTGCAGTATTCAAAGTATTTCAGAAAGAGCTTTTGCAGTGCTTTAGTTATCTTGGGTTTAAAATCTATGAAGAAGGGTCCCTTAGCAGATATTTGGTGCGTAAGTGTGGAAATGATGATATGGTGAAGCATGTAGTTACATTCAATGCATCCAATCTTGATATCAGTTGTAGCTGTCAGATGTTTGAATATGAAGGTGTTCTTTGTAGACATGTGTTGAGGGTTTTTCAAATATTGGAAGTAAGAGAAGTTCCTTCTCGCTACATCTTACACCGATGGACGAGAAATGCTGAGGATGGTGTTTTTCCCGACATAGGATCATGGAGTAGCTCTCAGGAGCTAAAGAGTTTGATGCGATGGAGTCTAAGAGAAACAGCATCTAAATACATAGATGCTGGTGCATCATCAATTGAAAAGTATAAACTTGCCTACGAGATTTTACGAGAGGGTGGGAGAAAACTTTGTTGGCACAGGTGA
Protein sequence

>Msa0621520

MLQFQVVGFGIMIVKEHPVGTELVMSNAIGEEEIDFSCDPYIGLEFFNADDALKFYISYGNRMGFKVRIGQLYRSRTNGSVSSRRFVCSKEGHQLSSRTGCPAFIRVQLNDSGKWVVDHFHKDHNHTLENESESFAPTLQPKASSAIESSTEVTRKPRKKMLEVGNGEPISPFGVINFKRMRKEELEVQARIEPYVGQEFSTPNEAYQFYHAHAAYLGFGIRVGQLFRSKNDGSITSRRFVCSKEGFQHPSRVGCKAYLRIKNQPSGKWVVDRLEKDHNHDLGLEKERKTKSLPASNILTEEVNTELVNGDIFRIDNYPVLRGARQNHIRSDWYNMLLEYFQSRQAEDTGFFYAMEVDNGNCMSIFWADGRSRYSCSQFGDVLVVDTSYRKSVYMVPFATFVGVNHHKQPVLLGCALIADESEESFTWLFQTWVRAMSGRQPLSVIADQDVSIQRAIAKVFPVTNHRFSLWQINAKEQELAGLMGNGFTKDYENCVYQSQTVDEFDASWNAVLVKYGLKDNAWLKEMYEKRASWVPFYLKGTFSAGIPMKESMESFFGGSLNGQTPLIEFIPRYERGLERRREEERKEDFNTSNFQTFLQTKEPVEEQCRRLYTLAVFKVFQKELLQCFSYLGFKIYEEGSLSRYLVRKCGNDDMVKHVVTFNASNLDISCSCQMFEYEGVLCRHVLRVFQILEVREVPSRYILHRWTRNAEDGVFPDIGSWSSSQELKSLMRWSLRETASKYIDAGASSIEKYKLAYEILREGGRKLCWHR*