AlfalfaGEDB | Msa0630530

M. sativa cultivar zhongmu-4 / Msa0630530

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630530	XP_003604788.2	99.446	542	3	0	1	542	1	542	0.0	1102

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630530	sp\|Q8H186\|PBL1_ARATH	38.636	308	177	6	153	451	45	349	1.85e-62	213

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630530	A0A396I2K1	99.446	542	3	0	1	542	1	542	0.0	1102

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630530	MtrunA17_Chr4g0008091	99.446	542	3	0	1	542	1	542	0.0	1102
Msa0630530	MtrunA17_Chr7g0244861	81.785	549	90	5	1	542	1	546	0.0	835
Msa0630530	MtrunA17_Chr4g0012871	62.931	464	145	10	75	528	127	573	4.99e-179	516
Msa0630530	MtrunA17_Chr5g0416311	39.316	351	195	10	153	491	60	404	1.71e-66	221
Msa0630530	MtrunA17_Chr5g0408941	41.083	314	172	7	146	453	69	375	6.55e-64	214
Msa0630530	MtrunA17_Chr8g0388941	40.333	300	167	7	157	450	59	352	1.84e-63	212
Msa0630530	MtrunA17_Chr7g0264461	35.952	331	192	6	153	466	78	405	2.28e-62	211
Msa0630530	MtrunA17_Chr1g0180951	38.816	304	176	5	153	449	73	373	2.85e-62	210
Msa0630530	MtrunA17_Chr7g0216551	37.654	324	189	5	137	453	94	411	1.00e-61	210
Msa0630530	MtrunA17_Chr7g0238671	39.172	314	178	7	153	457	57	366	3.22e-61	207

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630530	AT3G08760.3	59.640	555	178	14	1	526	3	540	0.0	586
Msa0630530	AT3G08760.1	59.640	555	178	14	1	526	3	540	0.0	586
Msa0630530	AT3G08760.2	59.640	555	178	14	1	526	3	540	0.0	586
Msa0630530	AT2G28940.2	38.141	312	185	5	148	451	81	392	5.76e-64	216
Msa0630530	AT3G55450.1	38.636	308	177	6	153	451	45	349	1.88e-63	213
Msa0630530	AT3G55450.2	38.636	308	177	6	153	451	82	386	9.82e-63	212
Msa0630530	AT5G02290.2	36.494	348	202	8	151	489	50	387	1.94e-62	210
Msa0630530	AT5G02290.1	36.494	348	202	8	151	489	50	387	1.94e-62	210
Msa0630530	AT1G14370.1	36.243	378	206	10	144	491	54	426	2.66e-62	211
Msa0630530	AT5G15080.1	39.088	307	176	5	154	453	127	429	3.50e-62	212

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Find 169 sgRNAs with CRISPR-Local

Find 387 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGCGTAAGCCCTGGCG+AGG	0.668387	4_3:-12471210	Msa0630530:CDS
AAACCTATCGATGGAGACAC+TGG	0.568870	4_3:-12469137	Msa0630530:CDS
AAACTCCATACATTGCTCTT+CGG	0.542880	4_3:+12469097	None:intergenic
AAAGGAAGCTTTATACATGG+TGG	0.661487	4_3:+12470728	None:intergenic
AAAGGTCCAGAAGCATATAA+AGG	0.459116	4_3:+12470855	None:intergenic
AAGAGAGAAATTTGGTGAAG+TGG	0.459703	4_3:-12469012	Msa0630530:CDS
AAGAGCAATGTATGGAGTTT+CGG	0.398674	4_3:-12469094	Msa0630530:CDS
AAGCTGCGAAGTGAAGGTTT+TGG	0.466866	4_3:+12473409	None:intergenic
AAGTGAGCGAGTATGGGTTT+GGG	0.382339	4_3:+12471163	None:intergenic
AAGTTTCGCAGTGACCCAGT+TGG	0.669758	4_3:+12470822	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTGAAGAAATGGGTAAAAA+TGG	+	chr4_3:12468406-12468425	None:intergenic	30.0%
AGAAAATTGAACAAACCTGA+AGG	+	chr4_3:12468470-12468489	None:intergenic	30.0%
TTCATGTAGCTGAATTATTG+TGG	-	chr4_3:12468752-12468771	Msa0630530:CDS	30.0%
TTCATGCAGTTAAACTAGTA+AGG	-	chr4_3:12468781-12468800	Msa0630530:CDS	30.0%
AATCGCAAATGAGGTTTATA+AGG	-	chr4_3:12468977-12468996	Msa0630530:CDS	30.0%
AGGAATGGAGAAAAAAAGTT+TGG	-	chr4_3:12469221-12469240	Msa0630530:intron	30.0%
AATTGCATGAGTTACGATTT+AGG	-	chr4_3:12469309-12469328	Msa0630530:CDS	30.0%
TTTCATGTGACCTATATTGT+TGG	+	chr4_3:12469360-12469379	None:intergenic	30.0%
TTAAGATTCCTATGATTCCA+GGG	+	chr4_3:12469434-12469453	None:intergenic	30.0%
ATTAAGATTCCTATGATTCC+AGG	+	chr4_3:12469435-12469454	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	12468262	12473481	12468262	ID=Msa0630530;Name=Msa0630530
chr4_3	mRNA	12468262	12473481	12468262	ID=Msa0630530-mRNA-1;Parent=Msa0630530;Name=Msa0630530-mRNA-1;_AED=0.06;_eAED=0.06;_QI=253\|1\|1\|1\|0.75\|0.6\|5\|291\|542
chr4_3	exon	12468262	12469167	12468262	ID=Msa0630530-mRNA-1:exon:3642;Parent=Msa0630530-mRNA-1
chr4_3	exon	12469302	12469421	12469302	ID=Msa0630530-mRNA-1:exon:3641;Parent=Msa0630530-mRNA-1
chr4_3	exon	12469802	12470059	12469802	ID=Msa0630530-mRNA-1:exon:3640;Parent=Msa0630530-mRNA-1
chr4_3	exon	12470656	12471332	12470656	ID=Msa0630530-mRNA-1:exon:3639;Parent=Msa0630530-mRNA-1
chr4_3	exon	12473270	12473481	12473270	ID=Msa0630530-mRNA-1:exon:3638;Parent=Msa0630530-mRNA-1
chr4_3	five_prime_UTR	12473270	12473481	12473270	ID=Msa0630530-mRNA-1:five_prime_utr;Parent=Msa0630530-mRNA-1
chr4_3	five_prime_UTR	12471292	12471332	12471292	ID=Msa0630530-mRNA-1:five_prime_utr;Parent=Msa0630530-mRNA-1
chr4_3	CDS	12470656	12471291	12470656	ID=Msa0630530-mRNA-1:cds;Parent=Msa0630530-mRNA-1
chr4_3	CDS	12469802	12470059	12469802	ID=Msa0630530-mRNA-1:cds;Parent=Msa0630530-mRNA-1
chr4_3	CDS	12469302	12469421	12469302	ID=Msa0630530-mRNA-1:cds;Parent=Msa0630530-mRNA-1
chr4_3	CDS	12468553	12469167	12468553	ID=Msa0630530-mRNA-1:cds;Parent=Msa0630530-mRNA-1
chr4_3	three_prime_UTR	12468262	12468552	12468262	ID=Msa0630530-mRNA-1:three_prime_utr;Parent=Msa0630530-mRNA-1

Gene Sequence

>Msa0630530

ATGGGGTGTTTTACTATATTGAAGAGAAAGAAGAAAAAGCCTGATCAGATTGTGTATGTAAAACGCGTAAGCCCTGGCGAGGATTCACCTACTGTACTGCCCGAACCCCAAACCCATACTCGCTCACTTCAGTCTGCGCCTCCTAGTTTTAAGATCAGAGTGAAACCCATTCAACCTAGCAATAAAGCCACTAATAATAGAATACGAGCATTGTCTGCTCCATCGAGTCTTGATGATGCAGAACAAGATGCATTGGCTACCATTGAGTATGAAGAACAAGAAGGGTCAAAATACCGAACTGGATCATGGAAGGAGCAGCGTTCGCCTAGTCCACAACCATTACCGCTTCCATCTCCTAAGGGTGGTGGTACACTGAAGACTGTTGGAAGCTTTAAGTTGGGGACAGCTAGTAGTCCTTTATATGCTTCTGGACCTTTGCCGCTTCCACCAACTGGGTCACTGCGAAACTTTTCTTATGATGAACTTGCTGCTGCTTGCCTCAATTTCTCTTCAGATCGATACATGTCAGAATCTCTTTCATCCACCATGTATAAAGCTTCCTTTGGTGATGATACCTCAACTTCAAGTTCAAAGAAGTTTGAAGCTACCGTCACACGCCTTCGCCCATCATCTCAGGGCCTGAAGGAATTCATAAATGAGGTTAATACTCTTGCATCATTGCAGCATCCAAACCTCTGTAGATTGCTAGGATTTCACGCAGGTGATGGTTCAGAGCATAGAATGTTGGTTTATGAGAGGCTATACCATGGAAGCTTGGACCGCTTATTGTATGGGAGATCTGATGGACCATCAATTGATTGGAATACAAGAATGAAAATTGCAATATGTGCTGCACTAGGTCTTTCTTTCTTGCATGAAGAAGGGCCTTTTCAGGCAATGTATAATGAATTTTCAACAGCTAACATTCAGATTGACAAAGATTTCAGTGCAAAGCTTTCAGGATATGGTTGTGTTGGACATGTTCCGAAGGAAGAGATTTCAAGCAGTTCATCTGCCGTTGGAAACCTATCGATGGAGACACTGGAGAAAGGAATGCTTACTCCGAAGAGCAATGTATGGAGTTTCGGAATTTTCCTTCTAGAGCTACTTACAGGAAGAAAGAATCTCGATAGCCGTCACCCCAAGGAAGAGAGAAATTTGGTGAAGTGGAGCAGGCCTTTCCTGTCTGATAATCATCGTTTGTCAATGATCATGGATCCTCAACTTAAAGGCCGCTTTCCTTCTAAAGCGGCGAGTACAATAGCTAACATTGCACAAAGATGCCTTCAAATGGAGCCATCAGAACGACCAACCATGGGAACAGTTGTTGAGCAGCTGAAAAAGATACAAGATTTGAAGCATTCTAGCAGATTCCCGCTGCAAGAACCAGCACAAATGTCGAGATCACCAAGTCTTAACGGTATCAACAATCCTGCACCAAGGCCAAGTTTCTCTCCATCACCATCATCCAGAGCCCTGGTATCTGTCTCACCTCCAAGATGGTCGGGAGTGTCAATTCAACTTCCGCCTCGTGCTTTTTCTTCAACCCTCTATTTGGAGGAGCTTGATAGGCAAGAAAGCCGCAAGTCACTGTCAGCCTCTAGGAAGGCTAGTGTTGAAGGATTTTGA

Protein sequence

>Msa0630530

MGCFTILKRKKKKPDQIVYVKRVSPGEDSPTVLPEPQTHTRSLQSAPPSFKIRVKPIQPSNKATNNRIRALSAPSSLDDAEQDALATIEYEEQEGSKYRTGSWKEQRSPSPQPLPLPSPKGGGTLKTVGSFKLGTASSPLYASGPLPLPPTGSLRNFSYDELAAACLNFSSDRYMSESLSSTMYKASFGDDTSTSSSKKFEATVTRLRPSSQGLKEFINEVNTLASLQHPNLCRLLGFHAGDGSEHRMLVYERLYHGSLDRLLYGRSDGPSIDWNTRMKIAICAALGLSFLHEEGPFQAMYNEFSTANIQIDKDFSAKLSGYGCVGHVPKEEISSSSSAVGNLSMETLEKGMLTPKSNVWSFGIFLLELLTGRKNLDSRHPKEERNLVKWSRPFLSDNHRLSMIMDPQLKGRFPSKAASTIANIAQRCLQMEPSERPTMGTVVEQLKKIQDLKHSSRFPLQEPAQMSRSPSLNGINNPAPRPSFSPSPSSRALVSVSPPRWSGVSIQLPPRAFSSTLYLEELDRQESRKSLSASRKASVEGF*