AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0631910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0631910 MtrunA17_Chr4g0009901 88.686 274 31 0 1 274 1 274 0.0 504
Msa0631910 MtrunA17_Chr4g0009851 78.008 241 52 1 1 240 1 241 1.47e-134 382
Msa0631910 MtrunA17_Chr4g0009981 76.596 188 44 0 53 240 1 188 5.67e-101 295
Msa0631910 MtrunA17_Chr4g0011071 81.022 137 26 0 55 191 35 171 5.53e-80 239
Msa0631910 MtrunA17_Chr1g0161431 45.490 255 108 3 2 225 3 257 6.47e-71 229
Msa0631910 MtrunA17_Chr4g0009991 82.906 117 19 1 101 217 1 116 4.07e-63 194
Msa0631910 MtrunA17_Chr4g0067981 40.964 249 121 2 10 232 31 279 7.01e-59 198
Msa0631910 MtrunA17_Chr2g0294911 42.083 240 115 2 10 225 29 268 7.26e-59 197
Msa0631910 MtrunA17_Chr6g0471041 47.414 232 114 5 10 238 14 240 1.18e-57 189
Msa0631910 MtrunA17_Chr3g0123131 40.984 244 116 3 10 225 13 256 4.60e-57 191
Msa0631910 MtrunA17_Chr8g0376881 39.245 265 126 4 9 238 24 288 1.95e-56 189
Msa0631910 MtrunA17_Chr4g0010001 83.146 89 15 0 1 89 1 89 4.21e-52 165
Msa0631910 MtrunA17_Chr1g0149841 40.081 247 120 3 7 225 2 248 5.98e-52 176
Msa0631910 MtrunA17_Chr8g0391911 40.244 246 121 3 10 229 9 254 6.95e-52 170
Msa0631910 MtrunA17_Chr7g0275401 38.000 250 126 4 7 227 2 251 1.43e-49 165
Msa0631910 MtrunA17_Chr5g0423031 83.146 89 15 0 1 89 1 89 3.94e-48 158
Msa0631910 MtrunA17_Chr5g0423031 59.259 81 31 1 184 262 80 160 8.03e-18 79.3
Msa0631910 MtrunA17_Chr3g0137101 37.500 248 123 3 10 225 8 255 1.04e-47 167
Msa0631910 MtrunA17_Chr7g0275891 36.293 259 124 5 7 224 2 260 1.88e-47 161
Msa0631910 MtrunA17_Chr1g0149851 35.849 265 127 5 7 228 2 266 1.70e-44 157
Msa0631910 MtrunA17_Chr4g0074401 35.060 251 133 5 10 231 17 266 2.38e-44 158
Msa0631910 MtrunA17_Chr4g0074411 34.818 247 136 4 10 231 18 264 6.16e-44 157
Msa0631910 MtrunA17_Chr6g0456631 45.752 153 81 1 10 160 9 161 2.20e-43 145
Msa0631910 MtrunA17_Chr2g0304841 33.071 254 133 3 10 226 18 271 3.20e-43 155
Msa0631910 MtrunA17_Chr8g0383961 41.880 234 105 7 1 229 1 208 1.39e-42 146
Msa0631910 MtrunA17_Chr3g0134461 36.667 210 106 4 10 217 8 192 8.37e-37 129
Msa0631910 MtrunA17_Chr3g0134481 35.071 211 101 3 10 219 8 183 2.39e-35 125
Msa0631910 MtrunA17_Chr4g0074421 35.912 181 94 3 59 217 2 182 1.66e-33 120
Msa0631910 MtrunA17_Chr1g0149831 37.297 185 86 3 68 225 1 182 1.22e-30 114
Msa0631910 MtrunA17_Chr6g0473731 34.247 146 82 2 89 224 10 151 3.63e-18 83.6
Msa0631910 MtrunA17_Chr5g0417521 61.818 55 20 1 168 221 15 69 2.69e-17 80.5
Msa0631910 MtrunA17_Chr6g0461641 63.636 55 19 1 168 221 42 96 9.13e-17 79.3
Msa0631910 MtrunA17_Chr2g0321461 53.623 69 30 2 154 221 32 99 8.21e-16 76.6
Msa0631910 MtrunA17_Chr8g0350761 25.177 282 162 10 12 246 19 298 4.36e-15 74.7
Msa0631910 MtrunA17_Chr2g0324891 43.617 94 50 3 133 224 125 217 1.54e-14 73.2
Msa0631910 MtrunA17_Chr7g0254981 44.318 88 46 2 159 243 41 128 1.61e-14 72.0
Msa0631910 MtrunA17_Chr7g0261911 51.429 70 33 1 168 236 262 331 1.82e-14 72.8
Msa0631910 MtrunA17_Chr7g0255711 55.932 59 26 0 167 225 90 148 2.60e-14 71.6
Msa0631910 MtrunA17_Chr4g0031961 49.275 69 35 0 157 225 120 188 2.68e-14 71.6
Msa0631910 MtrunA17_Chr4g0042451 56.364 55 23 1 168 221 17 71 3.67e-14 71.6
Msa0631910 MtrunA17_Chr7g0274471 61.111 54 20 1 168 220 218 271 7.70e-14 70.9
Msa0631910 MtrunA17_Chr1g0190301 56.897 58 24 1 164 220 171 228 1.11e-13 70.5
Msa0631910 MtrunA17_Chr2g0283951 43.373 83 41 3 150 230 75 153 1.20e-13 70.1
Msa0631910 MtrunA17_Chr1g0162931 50.000 68 32 2 154 220 5 71 1.37e-13 69.3
Msa0631910 MtrunA17_Chr4g0040481 55.172 58 26 0 167 224 157 214 1.40e-13 70.1
Msa0631910 MtrunA17_Chr1g0198601 47.500 80 34 2 149 227 199 271 2.26e-13 69.3
Msa0631910 MtrunA17_Chr4g0062921 46.753 77 39 2 158 233 226 301 2.73e-13 69.3
Msa0631910 MtrunA17_Chr5g0400241 46.667 75 36 1 167 241 310 380 3.40e-13 69.3
Msa0631910 MtrunA17_Chr5g0400241 28.571 126 85 3 10 133 14 136 2.02e-11 63.9
Msa0631910 MtrunA17_Chr4g0054981 55.357 56 25 0 167 222 309 364 4.53e-13 68.9
Msa0631910 MtrunA17_Chr7g0243081 51.786 56 26 1 166 220 69 124 7.81e-13 67.8
Msa0631910 MtrunA17_Chr3g0095611 50.000 64 31 1 158 220 9 72 9.94e-13 65.9
Msa0631910 MtrunA17_Chr1g0175091 52.632 57 26 1 166 221 256 312 1.65e-12 67.0
Msa0631910 MtrunA17_Chr7g0258551 53.571 56 25 1 166 220 210 265 2.04e-12 66.6
Msa0631910 MtrunA17_Chr2g0291021 58.182 55 22 1 168 221 49 103 2.18e-12 66.2
Msa0631910 MtrunA17_Chr1g0210911 47.143 70 34 2 154 220 6 75 3.24e-12 65.9
Msa0631910 MtrunA17_Chr8g0379121 43.836 73 35 2 149 220 97 164 5.69e-12 65.5
Msa0631910 MtrunA17_Chr8g0356501 44.444 81 40 2 164 239 33 113 6.22e-12 65.1
Msa0631910 MtrunA17_Chr2g0320231 43.243 74 38 2 148 220 80 150 7.32e-12 65.1
Msa0631910 MtrunA17_Chr2g0310011 40.000 100 51 3 164 254 31 130 8.04e-12 64.7
Msa0631910 MtrunA17_Chr2g0290991 49.315 73 35 2 164 235 35 106 1.10e-11 63.9
Msa0631910 MtrunA17_Chr4g0045361 46.377 69 36 1 158 225 264 332 1.26e-11 64.3
Msa0631910 MtrunA17_Chr5g0422621 52.459 61 28 1 167 226 197 257 1.51e-11 63.9
Msa0631910 MtrunA17_Chr1g0197171 54.237 59 26 1 164 221 40 98 1.71e-11 63.2
Msa0631910 MtrunA17_Chr1g0175071 54.098 61 27 1 164 223 173 233 1.81e-11 63.5
Msa0631910 MtrunA17_Chr5g0409731 46.377 69 34 2 164 229 21 89 2.75e-11 62.8
Msa0631910 MtrunA17_Chr1g0175081 45.614 57 30 1 166 221 20 76 3.31e-11 58.9
Msa0631910 MtrunA17_Chr2g0288601 49.153 59 29 1 160 217 63 121 3.58e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0631910 AT2G01760.1 40.000 260 117 5 7 229 4 261 1.05e-58 192
Msa0631910 AT1G67710.1 40.530 264 126 5 11 243 8 271 3.89e-53 181
Msa0631910 AT2G01760.2 35.294 306 129 7 7 245 4 307 7.96e-53 177
Msa0631910 AT3G16857.1 38.662 269 119 5 10 232 33 301 6.85e-52 180
Msa0631910 AT3G16857.2 38.662 269 119 5 10 232 33 301 8.73e-52 180
Msa0631910 AT2G25180.1 34.982 283 156 4 10 268 13 291 7.65e-48 168
Msa0631910 AT4G31920.1 37.500 232 133 3 10 229 13 244 2.40e-46 163
Msa0631910 AT5G58080.1 35.769 260 131 7 10 242 14 264 1.69e-39 145
Msa0631910 AT3G16857.3 37.391 230 98 5 49 232 2 231 3.79e-39 144
Msa0631910 AT2G01760.3 38.384 198 84 4 68 229 1 196 8.98e-38 135
Msa0631910 AT1G67710.2 40.291 206 93 4 68 243 1 206 8.88e-36 133
Msa0631910 AT3G62670.1 33.784 222 124 6 28 226 53 274 7.50e-33 125
Msa0631910 AT1G49190.1 30.303 264 151 8 10 242 30 291 6.78e-32 122
Msa0631910 AT5G49240.1 30.488 246 142 5 1 217 27 272 3.65e-31 117
Msa0631910 AT1G67710.3 39.247 186 83 4 88 243 12 197 2.74e-27 110
Msa0631910 AT5G07210.2 28.148 270 138 7 14 229 16 283 8.89e-25 103
Msa0631910 AT5G07210.1 27.338 278 138 8 14 229 16 291 2.73e-22 96.7
Msa0631910 AT1G68210.1 25.632 277 143 7 8 225 7 279 2.77e-18 85.1
Msa0631910 AT1G49190.2 32.000 125 84 1 10 133 30 154 2.98e-18 84.7
Msa0631910 AT2G27070.1 25.651 269 141 8 14 225 16 282 7.93e-17 80.5
Msa0631910 AT5G29000.4 37.302 126 64 4 103 220 166 284 1.83e-16 78.2
Msa0631910 AT3G04030.1 61.818 55 20 1 168 221 45 99 2.85e-16 78.2
Msa0631910 AT5G29000.1 42.157 102 51 3 120 220 147 241 2.99e-16 78.2
Msa0631910 AT5G29000.3 42.157 102 51 3 120 220 147 241 2.99e-16 78.2
Msa0631910 AT3G04030.3 61.818 55 20 1 168 221 45 99 3.14e-16 78.2
Msa0631910 AT5G18240.2 54.167 72 31 2 151 221 29 99 3.26e-16 78.2
Msa0631910 AT5G18240.3 54.167 72 31 2 151 221 29 99 3.26e-16 78.2
Msa0631910 AT5G29000.2 37.302 126 64 4 103 220 166 284 3.50e-16 78.2
Msa0631910 AT5G18240.1 54.167 72 31 2 151 221 29 99 3.72e-16 78.2
Msa0631910 AT5G18240.4 54.167 72 31 2 151 221 29 99 3.72e-16 78.2
Msa0631910 AT5G18240.5 54.167 72 31 2 151 221 29 99 3.79e-16 78.2
Msa0631910 AT5G59570.2 50.649 77 35 2 149 225 125 198 3.21e-15 74.3
Msa0631910 AT5G59570.1 50.649 77 35 2 149 225 125 198 3.21e-15 74.3
Msa0631910 AT3G04030.2 57.407 54 23 0 168 221 45 98 3.31e-15 75.1
Msa0631910 AT2G01060.1 49.398 83 39 2 157 236 4 86 7.12e-15 73.6
Msa0631910 AT5G05090.1 55.932 59 26 0 167 225 80 138 9.20e-15 72.8
Msa0631910 AT3G10760.1 55.932 59 26 0 167 225 104 162 2.21e-14 72.4
Msa0631910 AT2G40970.1 54.237 59 27 0 167 225 104 162 2.97e-14 71.2
Msa0631910 AT3G46640.3 54.237 59 27 0 167 225 143 201 3.38e-14 72.0
Msa0631910 AT3G46640.2 54.237 59 27 0 167 225 143 201 3.52e-14 71.6
Msa0631910 AT3G46640.1 54.237 59 27 0 167 225 143 201 3.52e-14 71.6
Msa0631910 AT5G44190.1 58.621 58 24 0 167 224 146 203 5.72e-14 71.6
Msa0631910 AT5G44190.2 58.621 58 24 0 167 224 146 203 6.54e-14 71.2
Msa0631910 AT3G04450.4 57.407 54 22 1 168 220 198 251 7.86e-14 71.2
Msa0631910 AT3G04450.2 57.407 54 22 1 168 220 198 251 7.86e-14 71.2
Msa0631910 AT3G04450.1 57.407 54 22 1 168 220 238 291 8.05e-14 71.2
Msa0631910 AT3G04450.3 57.407 54 22 1 168 220 238 291 1.15e-13 70.1
Msa0631910 AT2G40260.1 41.584 101 52 3 134 233 51 145 1.18e-13 70.9
Msa0631910 AT4G28610.1 60.000 55 21 1 168 221 225 279 1.42e-13 70.5
Msa0631910 AT1G25550.1 46.835 79 40 2 154 230 194 272 3.09e-13 69.3
Msa0631910 AT1G69580.1 40.909 88 45 3 168 254 34 115 3.28e-13 68.9
Msa0631910 AT1G69580.2 40.909 88 45 3 168 254 34 115 3.46e-13 68.9
Msa0631910 AT4G13640.1 40.594 101 41 3 168 267 37 119 3.60e-13 68.6
Msa0631910 AT4G13640.2 40.594 101 41 3 168 267 37 119 3.80e-13 68.6
Msa0631910 AT2G20570.1 52.308 65 31 0 160 224 145 209 5.72e-13 68.9
Msa0631910 AT1G14600.1 61.538 52 19 1 170 220 25 76 1.19e-12 66.6
Msa0631910 AT3G24120.1 37.755 98 52 2 168 256 41 138 1.24e-12 67.0
Msa0631910 AT3G24120.2 37.755 98 52 2 168 256 41 138 1.29e-12 67.0
Msa0631910 AT5G06800.3 58.182 55 22 1 168 221 192 246 2.10e-12 66.2
Msa0631910 AT5G06800.2 58.182 55 22 1 168 221 192 246 3.30e-12 66.2
Msa0631910 AT5G06800.1 58.182 55 22 1 168 221 192 246 3.46e-12 66.2
Msa0631910 AT4G04605.1 53.571 56 25 1 166 220 12 67 3.75e-12 64.7
Msa0631910 AT3G13040.3 53.571 56 25 1 167 221 240 295 4.10e-12 66.2
Msa0631910 AT3G13040.1 53.571 56 25 1 167 221 240 295 4.10e-12 66.2
Msa0631910 AT3G13040.2 53.571 56 25 1 167 221 240 295 4.10e-12 66.2
Msa0631910 AT2G20570.2 58.182 55 23 0 170 224 171 225 4.83e-12 66.2
Msa0631910 AT1G79430.2 43.678 87 43 2 168 253 34 115 5.77e-12 65.5
Msa0631910 AT2G20400.2 59.259 54 21 1 168 220 231 284 7.16e-12 65.5
Msa0631910 AT4G18020.3 50.000 66 32 1 159 224 288 352 7.21e-12 65.9
Msa0631910 AT4G18020.2 50.000 66 32 1 159 224 288 352 7.21e-12 65.9
Msa0631910 AT4G18020.1 50.000 66 32 1 159 224 288 352 7.21e-12 65.9
Msa0631910 AT4G18020.6 50.000 66 32 1 159 224 288 352 7.21e-12 65.9
Msa0631910 AT4G18020.9 50.000 66 32 1 159 224 288 352 7.21e-12 65.9
Msa0631910 AT2G20400.1 59.259 54 21 1 168 220 231 284 7.28e-12 65.5
Msa0631910 AT4G18020.8 50.000 66 32 1 159 224 288 352 7.38e-12 65.5
Msa0631910 AT4G18020.11 50.000 66 32 1 159 224 288 352 7.38e-12 65.5
Msa0631910 AT4G18020.4 50.000 66 32 1 159 224 288 352 7.38e-12 65.5
Msa0631910 AT4G18020.5 50.000 66 32 1 159 224 288 352 7.38e-12 65.5
Msa0631910 AT4G18020.10 50.000 66 32 1 159 224 288 352 7.38e-12 65.5
Msa0631910 AT4G18020.7 50.000 66 32 1 159 224 288 352 7.38e-12 65.5
Msa0631910 AT3G12730.1 37.755 98 58 2 168 264 23 118 7.50e-12 63.9
Msa0631910 AT5G60100.2 30.894 123 79 3 14 131 64 185 1.21e-11 65.1
Msa0631910 AT2G42660.1 46.032 63 33 1 159 220 41 103 1.30e-11 63.5
Msa0631910 AT5G60100.7 30.894 123 79 3 14 131 64 185 1.57e-11 64.3
Msa0631910 AT5G60100.6 30.894 123 79 3 14 131 64 185 1.57e-11 64.3
Msa0631910 AT5G60100.4 30.894 123 79 3 14 131 64 185 1.57e-11 64.3
Msa0631910 AT5G60100.5 30.894 123 79 3 14 131 64 185 1.76e-11 64.3
Msa0631910 AT5G60100.3 30.894 123 79 3 14 131 64 185 1.76e-11 64.3
Msa0631910 AT5G60100.1 30.894 123 79 3 14 131 64 185 1.76e-11 64.3
Msa0631910 AT2G03500.1 50.000 62 30 1 167 227 232 293 1.79e-11 64.3
Msa0631910 AT5G45580.1 52.542 59 27 1 164 221 3 61 2.70e-11 62.8
Msa0631910 AT5G45580.2 52.542 59 27 1 164 221 19 77 3.21e-11 62.8
Msa0631910 AT2G38300.1 48.214 56 28 1 166 220 52 107 4.85e-11 62.8

Find 64 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGCATCAACGATTTCTA+TGG 0.226165 4_3:-15342943 Msa0631910:CDS
CAAAATGATTCTCAAAATTA+TGG 0.243357 4_3:-15342796 Msa0631910:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4_3:-15342751 Msa0631910:intron
ATTATGGATGTTCCTGGTTT+AGG 0.300681 4_3:-15342780 Msa0631910:CDS
ATCCTACCTTTACAACCTTA+TGG 0.307805 4_3:-15342462 Msa0631910:CDS
CTCAAAATTATGGATGTTCC+TGG 0.317981 4_3:-15342786 Msa0631910:CDS
CTTGAATTTGTTGGAAAGAA+AGG 0.326280 4_3:-15343439 Msa0631910:CDS
AGAAATCGTTGATGCAATTC+TGG 0.327125 4_3:+15342947 None:intergenic
CTAAGAATTCTTTAATTAAA+CGG 0.349796 4_3:+15342431 None:intergenic
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4_3:-15343448 Msa0631910:CDS
AAAGTGACTAACCTTGATAA+TGG 0.378712 4_3:+15343606 None:intergenic
CTGAATGGATTGATCAAAAT+CGG 0.378920 4_3:-15342526 Msa0631910:CDS
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4_3:-15343156 Msa0631910:CDS
CGAATATTGGACTAAGCCTT+TGG 0.392176 4_3:-15343143 Msa0631910:CDS
CTTTCTTTCCAACAAATTCA+AGG 0.396613 4_3:+15343440 None:intergenic
TCTTTAATTAAACGGCATCT+AGG 0.403700 4_3:+15342439 None:intergenic
ATCAGAAGCTGTGTTACATT+TGG 0.412873 4_3:+15343464 None:intergenic
AATAAAGAATATGAAATTGT+TGG 0.416766 4_3:-15343064 Msa0631910:CDS
TGATCAAGAAAATAGAAAAC+GGG 0.418887 4_3:-15343032 Msa0631910:CDS
TGGCAACATGTTATAAGAAA+AGG 0.422808 4_3:-15343097 Msa0631910:CDS
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4_3:-15342622 Msa0631910:intron
ATATGAAATTGTTGGAAGCT+TGG 0.432524 4_3:-15343056 Msa0631910:CDS
AATAAATTCTTACTGATGAC+TGG 0.451449 4_3:+15343327 None:intergenic
AACAGTGATGTCATTATCAA+TGG 0.466318 4_3:+15343661 None:intergenic
TTGAGACCAGCAGGGAATGA+TGG 0.467536 4_3:+15343692 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.468019 4_3:+15342964 None:intergenic
AAGTCATAAGCATCCATGTT+TGG 0.471983 4_3:+15343381 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_3:-15342973 Msa0631910:CDS
TTGATCAAGAAAATAGAAAA+CGG 0.477078 4_3:-15343033 Msa0631910:CDS
TGTACTCGATTCCATTATCA+AGG 0.477393 4_3:-15343617 Msa0631910:intron
AAAAGAAACACATGCAAAGA+AGG 0.478150 4_3:-15342987 Msa0631910:CDS
CACTGTTCTTAACACTATTG+AGG 0.478157 4_3:-15343645 Msa0631910:CDS
ACGATGACTTTCTAGCTGAA+TGG 0.481545 4_3:-15342502 Msa0631910:CDS
GAATGGATTGACTGTGGGGC+GGG 0.487587 4_3:-15342485 Msa0631910:CDS
TAGCTGAATGGATTGACTGT+GGG 0.492334 4_3:-15342490 Msa0631910:CDS
CTTCATAATTGAACTTTCTG+TGG 0.494002 4_3:+15343186 None:intergenic
GCAATAACCTTGAGACCAGC+AGG 0.494358 4_3:+15343683 None:intergenic
TAAACGGCATCTAGGCCATA+AGG 0.496018 4_3:+15342447 None:intergenic
ATTCTGGCGACCAAGACAAA+CGG 0.509916 4_3:+15342963 None:intergenic
AGGAAAGAATCATACTATCA+AGG 0.510494 4_3:+15342903 None:intergenic
TAGGATTCATTTGAAGAGAT+CGG 0.515087 4_3:-15342603 Msa0631910:CDS
GATGTTCCTGGTTTAGGTGT+AGG 0.515547 4_3:-15342774 Msa0631910:CDS
ATTATGAAGTCTATTGAATG+TGG 0.515558 4_3:-15343172 Msa0631910:CDS
CTATGGGCTGTCAATCAACT+AGG 0.518108 4_3:-15342926 Msa0631910:CDS
GTGATAGCTCTCAAGCTGAA+TGG 0.519299 4_3:-15342541 Msa0631910:CDS
ATCATTCCCTGCTGGTCTCA+AGG 0.520371 4_3:-15343690 Msa0631910:CDS
GACAACCCATCATTCCCTGC+TGG 0.534739 4_3:-15343698 Msa0631910:CDS
TGAGACCAGCAGGGAATGAT+GGG 0.539790 4_3:+15343693 None:intergenic
TTATGAAGTCTATTGAATGT+GGG 0.546749 4_3:-15343171 Msa0631910:CDS
ATTGCATCAACGATTTCTAT+GGG 0.552524 4_3:-15342942 Msa0631910:CDS
TCTAGGCCATAAGGTTGTAA+AGG 0.556671 4_3:+15342456 None:intergenic
GACTGGAATGTTGAGCTGTA+AGG 0.570940 4_3:+15343344 None:intergenic
CTAGCTGAATGGATTGACTG+TGG 0.582882 4_3:-15342491 Msa0631910:CDS
TCTTATAACATGTTGCCACA+TGG 0.594344 4_3:+15343102 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.601142 4_3:-15343170 Msa0631910:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4_3:+15342460 None:intergenic
TAATACTCATATGCCAAACA+TGG 0.605586 4_3:-15343394 Msa0631910:CDS
AGCTGAATGGATTGACTGTG+GGG 0.620097 4_3:-15342489 Msa0631910:CDS
TGAATGGATTGACTGTGGGG+CGG 0.657142 4_3:-15342486 Msa0631910:CDS
ACATGTCCTACACCTAAACC+AGG 0.664952 4_3:+15342768 None:intergenic
CGGCTGATGAAATAAAACAC+GGG 0.670080 4_3:-15342583 Msa0631910:CDS
TCGGCTGATGAAATAAAACA+CGG 0.697835 4_3:-15342584 Msa0631910:CDS
CAATAACCTTGAGACCAGCA+GGG 0.699486 4_3:+15343684 None:intergenic
GATCAAGAAAATAGAAAACG+GGG 0.722642 4_3:-15343031 Msa0631910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG - chr4_3:15343089-15343108 Msa0631910:CDS 15.0%
!! AAAAATAAGTGAGATAAAAA+TGG + chr4_3:15343324-15343343 None:intergenic 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG + chr4_3:15343358-15343377 None:intergenic 15.0%
!! AATAAAGAATATGAAATTGT+TGG - chr4_3:15343089-15343108 Msa0631910:CDS 15.0%
!! AAAAATAAGTGAGATAAAAA+TGG + chr4_3:15343324-15343343 None:intergenic 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG + chr4_3:15343358-15343377 None:intergenic 15.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG - chr4_3:15342935-15342954 Msa0631910:CDS 20.0%
!! TTGATCAAGAAAATAGAAAA+CGG - chr4_3:15343120-15343139 Msa0631910:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG + chr4_3:15343359-15343378 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG - chr4_3:15343357-15343376 Msa0631910:CDS 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG - chr4_3:15342935-15342954 Msa0631910:CDS 20.0%
!! TTGATCAAGAAAATAGAAAA+CGG - chr4_3:15343120-15343139 Msa0631910:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG + chr4_3:15343359-15343378 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG - chr4_3:15343357-15343376 Msa0631910:CDS 20.0%
! AATAAATTCTTACTGATGAC+TGG + chr4_3:15342829-15342848 None:intergenic 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4_3:15342981-15343000 Msa0631910:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4_3:15342982-15343001 Msa0631910:CDS 25.0%
! TGATCAAGAAAATAGAAAAC+GGG - chr4_3:15343121-15343140 Msa0631910:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG + chr4_3:15343273-15343292 None:intergenic 25.0%
! AATAAATTCTTACTGATGAC+TGG + chr4_3:15342829-15342848 None:intergenic 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4_3:15342981-15343000 Msa0631910:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4_3:15342982-15343001 Msa0631910:CDS 25.0%
! TGATCAAGAAAATAGAAAAC+GGG - chr4_3:15343121-15343140 Msa0631910:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG + chr4_3:15343273-15343292 None:intergenic 25.0%
AACAGTGATGTCATTATCAA+TGG + chr4_3:15342495-15342514 None:intergenic 30.0%
AAAGTGACTAACCTTGATAA+TGG + chr4_3:15342550-15342569 None:intergenic 30.0%
CTTTCTTTCCAACAAATTCA+AGG + chr4_3:15342716-15342735 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+AGG - chr4_3:15342714-15342733 Msa0631910:intron 30.0%
TAATACTCATATGCCAAACA+TGG - chr4_3:15342759-15342778 Msa0631910:CDS 30.0%
CTTCATAATTGAACTTTCTG+TGG + chr4_3:15342970-15342989 None:intergenic 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4_3:15342983-15343002 Msa0631910:CDS 30.0%
!! TTGAACTGTTTTTCAACCAA+AGG + chr4_3:15343029-15343048 None:intergenic 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4_3:15343056-15343075 Msa0631910:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG - chr4_3:15343097-15343116 Msa0631910:CDS 30.0%
GATCAAGAAAATAGAAAACG+GGG - chr4_3:15343122-15343141 Msa0631910:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG - chr4_3:15343166-15343185 Msa0631910:CDS 30.0%
AATTGCATCAACGATTTCTA+TGG - chr4_3:15343210-15343229 Msa0631910:intron 30.0%
ATTGCATCAACGATTTCTAT+GGG - chr4_3:15343211-15343230 Msa0631910:intron 30.0%
AGGAAAGAATCATACTATCA+AGG + chr4_3:15343253-15343272 None:intergenic 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG - chr4_3:15343413-15343432 Msa0631910:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG - chr4_3:15343531-15343550 Msa0631910:intron 30.0%
TAGGATTCATTTGAAGAGAT+CGG - chr4_3:15343550-15343569 Msa0631910:intron 30.0%
!! CTGAATGGATTGATCAAAAT+CGG - chr4_3:15343627-15343646 Msa0631910:CDS 30.0%
AACAGTGATGTCATTATCAA+TGG + chr4_3:15342495-15342514 None:intergenic 30.0%
AAAGTGACTAACCTTGATAA+TGG + chr4_3:15342550-15342569 None:intergenic 30.0%
CTTTCTTTCCAACAAATTCA+AGG + chr4_3:15342716-15342735 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+AGG - chr4_3:15342714-15342733 Msa0631910:intron 30.0%
TAATACTCATATGCCAAACA+TGG - chr4_3:15342759-15342778 Msa0631910:CDS 30.0%
CTTCATAATTGAACTTTCTG+TGG + chr4_3:15342970-15342989 None:intergenic 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4_3:15342983-15343002 Msa0631910:CDS 30.0%
!! TTGAACTGTTTTTCAACCAA+AGG + chr4_3:15343029-15343048 None:intergenic 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4_3:15343056-15343075 Msa0631910:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG - chr4_3:15343097-15343116 Msa0631910:CDS 30.0%
GATCAAGAAAATAGAAAACG+GGG - chr4_3:15343122-15343141 Msa0631910:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG - chr4_3:15343166-15343185 Msa0631910:CDS 30.0%
AATTGCATCAACGATTTCTA+TGG - chr4_3:15343210-15343229 Msa0631910:intron 30.0%
ATTGCATCAACGATTTCTAT+GGG - chr4_3:15343211-15343230 Msa0631910:intron 30.0%
AGGAAAGAATCATACTATCA+AGG + chr4_3:15343253-15343272 None:intergenic 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG - chr4_3:15343413-15343432 Msa0631910:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG - chr4_3:15343531-15343550 Msa0631910:intron 30.0%
TAGGATTCATTTGAAGAGAT+CGG - chr4_3:15343550-15343569 Msa0631910:intron 30.0%
!! CTGAATGGATTGATCAAAAT+CGG - chr4_3:15343627-15343646 Msa0631910:CDS 30.0%
CACTGTTCTTAACACTATTG+AGG - chr4_3:15342508-15342527 Msa0631910:CDS 35.0%
TGTACTCGATTCCATTATCA+AGG - chr4_3:15342536-15342555 Msa0631910:CDS 35.0%
ATCAGAAGCTGTGTTACATT+TGG + chr4_3:15342692-15342711 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG - chr4_3:15342705-15342724 Msa0631910:intron 35.0%
AAGTCATAAGCATCCATGTT+TGG + chr4_3:15342775-15342794 None:intergenic 35.0%
! TGAGCTGTAAGGTAACTTTT+TGG + chr4_3:15342801-15342820 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG - chr4_3:15343036-15343055 Msa0631910:CDS 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4_3:15343054-15343073 None:intergenic 35.0%
AGAAATCGTTGATGCAATTC+TGG + chr4_3:15343209-15343228 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+TGG - chr4_3:15343367-15343386 Msa0631910:CDS 35.0%
ATTATGGATGTTCCTGGTTT+AGG - chr4_3:15343373-15343392 Msa0631910:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr4_3:15343402-15343421 Msa0631910:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG - chr4_3:15343417-15343436 Msa0631910:CDS 35.0%
TCGGCTGATGAAATAAAACA+CGG - chr4_3:15343569-15343588 Msa0631910:intron 35.0%
ACGGGAAGAAAAAAACTAAG+TGG - chr4_3:15343588-15343607 Msa0631910:intron 35.0%
ATCCTACCTTTACAACCTTA+TGG - chr4_3:15343691-15343710 Msa0631910:CDS 35.0%
CACTGTTCTTAACACTATTG+AGG - chr4_3:15342508-15342527 Msa0631910:CDS 35.0%
TGTACTCGATTCCATTATCA+AGG - chr4_3:15342536-15342555 Msa0631910:CDS 35.0%
ATCAGAAGCTGTGTTACATT+TGG + chr4_3:15342692-15342711 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG - chr4_3:15342705-15342724 Msa0631910:intron 35.0%
AAGTCATAAGCATCCATGTT+TGG + chr4_3:15342775-15342794 None:intergenic 35.0%
! TGAGCTGTAAGGTAACTTTT+TGG + chr4_3:15342801-15342820 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG - chr4_3:15343036-15343055 Msa0631910:CDS 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4_3:15343054-15343073 None:intergenic 35.0%
AGAAATCGTTGATGCAATTC+TGG + chr4_3:15343209-15343228 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+TGG - chr4_3:15343367-15343386 Msa0631910:CDS 35.0%
ATTATGGATGTTCCTGGTTT+AGG - chr4_3:15343373-15343392 Msa0631910:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr4_3:15343402-15343421 Msa0631910:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG - chr4_3:15343417-15343436 Msa0631910:CDS 35.0%
TCGGCTGATGAAATAAAACA+CGG - chr4_3:15343569-15343588 Msa0631910:intron 35.0%
ACGGGAAGAAAAAAACTAAG+TGG - chr4_3:15343588-15343607 Msa0631910:intron 35.0%
ATCCTACCTTTACAACCTTA+TGG - chr4_3:15343691-15343710 Msa0631910:CDS 35.0%
CGAATATTGGACTAAGCCTT+TGG - chr4_3:15343010-15343029 Msa0631910:CDS 40.0%
CGGCTGATGAAATAAAACAC+GGG - chr4_3:15343570-15343589 Msa0631910:intron 40.0%
!! ACGATGACTTTCTAGCTGAA+TGG - chr4_3:15343651-15343670 Msa0631910:CDS 40.0%
!! TAGCTGAATGGATTGACTGT+GGG - chr4_3:15343663-15343682 Msa0631910:CDS 40.0%
TCTAGGCCATAAGGTTGTAA+AGG + chr4_3:15343700-15343719 None:intergenic 40.0%
CGAATATTGGACTAAGCCTT+TGG - chr4_3:15343010-15343029 Msa0631910:CDS 40.0%
CGGCTGATGAAATAAAACAC+GGG - chr4_3:15343570-15343589 Msa0631910:intron 40.0%
!! ACGATGACTTTCTAGCTGAA+TGG - chr4_3:15343651-15343670 Msa0631910:CDS 40.0%
!! TAGCTGAATGGATTGACTGT+GGG - chr4_3:15343663-15343682 Msa0631910:CDS 40.0%
TCTAGGCCATAAGGTTGTAA+AGG + chr4_3:15343700-15343719 None:intergenic 40.0%
CAATAACCTTGAGACCAGCA+GGG + chr4_3:15342472-15342491 None:intergenic 45.0%
GACTGGAATGTTGAGCTGTA+AGG + chr4_3:15342812-15342831 None:intergenic 45.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4_3:15342997-15343016 Msa0631910:CDS 45.0%
ATTCTGGCGACCAAGACAAA+CGG + chr4_3:15343193-15343212 None:intergenic 45.0%
! CTATGGGCTGTCAATCAACT+AGG - chr4_3:15343227-15343246 Msa0631910:intron 45.0%
GATGTTCCTGGTTTAGGTGT+AGG - chr4_3:15343379-15343398 Msa0631910:CDS 45.0%
ACATGTCCTACACCTAAACC+AGG + chr4_3:15343388-15343407 None:intergenic 45.0%
GTGATAGCTCTCAAGCTGAA+TGG - chr4_3:15343612-15343631 Msa0631910:intron 45.0%
!! CTAGCTGAATGGATTGACTG+TGG - chr4_3:15343662-15343681 Msa0631910:CDS 45.0%
!! AGCTGAATGGATTGACTGTG+GGG - chr4_3:15343664-15343683 Msa0631910:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG + chr4_3:15343696-15343715 None:intergenic 45.0%
TAAACGGCATCTAGGCCATA+AGG + chr4_3:15343709-15343728 None:intergenic 45.0%
CAATAACCTTGAGACCAGCA+GGG + chr4_3:15342472-15342491 None:intergenic 45.0%
GACTGGAATGTTGAGCTGTA+AGG + chr4_3:15342812-15342831 None:intergenic 45.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4_3:15342997-15343016 Msa0631910:CDS 45.0%
ATTCTGGCGACCAAGACAAA+CGG + chr4_3:15343193-15343212 None:intergenic 45.0%
! CTATGGGCTGTCAATCAACT+AGG - chr4_3:15343227-15343246 Msa0631910:intron 45.0%
GATGTTCCTGGTTTAGGTGT+AGG - chr4_3:15343379-15343398 Msa0631910:CDS 45.0%
ACATGTCCTACACCTAAACC+AGG + chr4_3:15343388-15343407 None:intergenic 45.0%
GTGATAGCTCTCAAGCTGAA+TGG - chr4_3:15343612-15343631 Msa0631910:intron 45.0%
!! CTAGCTGAATGGATTGACTG+TGG - chr4_3:15343662-15343681 Msa0631910:CDS 45.0%
!! AGCTGAATGGATTGACTGTG+GGG - chr4_3:15343664-15343683 Msa0631910:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG + chr4_3:15343696-15343715 None:intergenic 45.0%
TAAACGGCATCTAGGCCATA+AGG + chr4_3:15343709-15343728 None:intergenic 45.0%
TGAGACCAGCAGGGAATGAT+GGG + chr4_3:15342463-15342482 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG + chr4_3:15342464-15342483 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG - chr4_3:15342463-15342482 Msa0631910:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG + chr4_3:15342473-15342492 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4_3:15343180-15343199 Msa0631910:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG + chr4_3:15343192-15343211 None:intergenic 50.0%
!! TGAATGGATTGACTGTGGGG+CGG - chr4_3:15343667-15343686 Msa0631910:CDS 50.0%
TGAGACCAGCAGGGAATGAT+GGG + chr4_3:15342463-15342482 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG + chr4_3:15342464-15342483 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG - chr4_3:15342463-15342482 Msa0631910:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG + chr4_3:15342473-15342492 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4_3:15343180-15343199 Msa0631910:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG + chr4_3:15343192-15343211 None:intergenic 50.0%
!! TGAATGGATTGACTGTGGGG+CGG - chr4_3:15343667-15343686 Msa0631910:CDS 50.0%
GACAACCCATCATTCCCTGC+TGG - chr4_3:15342455-15342474 Msa0631910:exon 55.0%
!! GAATGGATTGACTGTGGGGC+GGG - chr4_3:15343668-15343687 Msa0631910:CDS 55.0%
GACAACCCATCATTCCCTGC+TGG - chr4_3:15342455-15342474 Msa0631910:exon 55.0%
!! GAATGGATTGACTGTGGGGC+GGG - chr4_3:15343668-15343687 Msa0631910:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 15342440 15343735 15342440 ID=Msa0631910;Name=Msa0631910
chr4_3 mRNA 15342440 15343735 15342440 ID=Msa0631910-mRNA-1;Parent=Msa0631910;Name=Msa0631910-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0.25|0.2|1|1|1|5|17|274
chr4_3 exon 15342440 15342630 15342440 ID=Msa0631910-mRNA-1:exon:4361;Parent=Msa0631910-mRNA-1
chr4_3 exon 15342752 15342828 15342752 ID=Msa0631910-mRNA-1:exon:4360;Parent=Msa0631910-mRNA-1
chr4_3 exon 15342918 15343223 15342918 ID=Msa0631910-mRNA-1:exon:4359;Parent=Msa0631910-mRNA-1
chr4_3 exon 15343340 15343489 15343340 ID=Msa0631910-mRNA-1:exon:4358;Parent=Msa0631910-mRNA-1
chr4_3 exon 15343618 15343735 15343618 ID=Msa0631910-mRNA-1:exon:4357;Parent=Msa0631910-mRNA-1
chr4_3 CDS 15343618 15343735 15343618 ID=Msa0631910-mRNA-1:cds;Parent=Msa0631910-mRNA-1
chr4_3 CDS 15343340 15343489 15343340 ID=Msa0631910-mRNA-1:cds;Parent=Msa0631910-mRNA-1
chr4_3 CDS 15342918 15343223 15342918 ID=Msa0631910-mRNA-1:cds;Parent=Msa0631910-mRNA-1
chr4_3 CDS 15342752 15342828 15342752 ID=Msa0631910-mRNA-1:cds;Parent=Msa0631910-mRNA-1
chr4_3 CDS 15342457 15342630 15342457 ID=Msa0631910-mRNA-1:cds;Parent=Msa0631910-mRNA-1
chr4_3 three_prime_UTR 15342440 15342456 15342440 ID=Msa0631910-mRNA-1:three_prime_utr;Parent=Msa0631910-mRNA-1
Gene Sequence

>Msa0631910

ATGGCTTCTTTCATTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATAATGACATCACTGTTCTTAACACTATTGAGGATATGTGTACTCGATTCCATTATCAAGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTAATACTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAAAGTTACCTTACAGCTCAACATTCCAGTCATCATGATGGCTGTTGATTCCACAGAAAGTTCAATTATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAACAGTTCAAGACCATGTGGCAACATGTTATAAGAAAAGGTTTGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTTGATCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGTCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAATTGCATCAACGATTTCTATGGGCTGTCAATCAACTAGGCCTTGATAAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCTGGTTTAGGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAATAAAACACGGGAAGAAAAAAACTAAGTGGAGTGATAGCTCTCAAGCTGAATGGATTGATCAAAATCGGAACGATGACTTTCTAGCTGAATGGATTGACTGTGGGGCGGGATCCTACCTTTACAACCTTATGGCCTAG
Protein sequence

>Msa0631910

MASFIDNPSFPAGLKVIAIDNDITVLNTIEDMCTRFHYQVTKCNTASDALNLLERKDCFDVMLINTHMPNMDAYDFVQKVTLQLNIPVIMMAVDSTESSIMKSIECGACEYWTKPLVEKQFKTMWQHVIRKGLTENKEYEIVGSLVDQENRKRGREDANVSKETHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADEIKHGKKKTKWSDSSQAEWIDQNRNDDFLAEWIDCGAGSYLYNLMA*