AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0631930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0631930 MtrunA17_Chr4g0009851 87.500 312 38 1 1 311 1 312 0.0 565
Msa0631930 MtrunA17_Chr4g0009981 84.170 259 41 0 53 311 1 259 5.55e-166 461
Msa0631930 MtrunA17_Chr4g0009901 71.250 240 69 0 1 240 1 240 4.65e-121 348
Msa0631930 MtrunA17_Chr4g0011071 78.102 137 30 0 55 191 35 171 1.78e-76 231
Msa0631930 MtrunA17_Chr6g0471041 46.711 304 141 8 1 294 1 293 8.49e-74 233
Msa0631930 MtrunA17_Chr1g0161431 36.944 360 162 8 13 307 14 373 3.64e-66 218
Msa0631930 MtrunA17_Chr3g0123131 40.678 236 112 3 13 220 16 251 6.77e-58 195
Msa0631930 MtrunA17_Chr4g0009991 76.068 117 27 1 101 217 1 116 1.57e-56 179
Msa0631930 MtrunA17_Chr4g0067981 41.026 234 112 2 13 220 34 267 1.97e-56 193
Msa0631930 MtrunA17_Chr2g0294911 38.433 268 139 3 13 254 32 299 2.94e-56 191
Msa0631930 MtrunA17_Chr8g0376881 40.171 234 114 3 13 220 28 261 1.05e-55 189
Msa0631930 MtrunA17_Chr7g0275401 37.705 244 123 3 13 227 8 251 6.55e-51 170
Msa0631930 MtrunA17_Chr1g0149841 39.831 236 114 3 13 220 8 243 9.48e-50 172
Msa0631930 MtrunA17_Chr8g0391911 40.435 230 111 3 14 217 13 242 9.69e-49 163
Msa0631930 MtrunA17_Chr3g0137101 39.583 240 113 4 13 220 11 250 5.42e-48 170
Msa0631930 MtrunA17_Chr8g0383961 42.481 266 115 8 1 254 1 240 1.49e-47 160
Msa0631930 MtrunA17_Chr7g0275891 34.007 297 151 7 13 266 8 302 4.77e-45 156
Msa0631930 MtrunA17_Chr1g0149851 33.444 302 157 6 13 270 8 309 4.37e-43 155
Msa0631930 MtrunA17_Chr5g0423031 71.910 89 25 0 1 89 1 89 9.52e-43 145
Msa0631930 MtrunA17_Chr4g0074401 32.794 247 138 4 13 231 20 266 1.99e-42 154
Msa0631930 MtrunA17_Chr4g0010001 71.910 89 25 0 1 89 1 89 2.16e-42 141
Msa0631930 MtrunA17_Chr2g0304841 34.524 252 128 4 13 227 21 272 3.05e-42 154
Msa0631930 MtrunA17_Chr4g0074411 33.607 244 137 4 13 231 21 264 5.80e-42 153
Msa0631930 MtrunA17_Chr3g0134461 39.614 207 98 4 13 217 11 192 1.60e-40 140
Msa0631930 MtrunA17_Chr3g0134481 34.842 221 106 3 4 223 4 187 2.71e-38 134
Msa0631930 MtrunA17_Chr6g0456631 43.919 148 81 1 14 159 13 160 9.30e-38 132
Msa0631930 MtrunA17_Chr4g0074421 36.464 181 93 3 59 217 2 182 1.75e-36 129
Msa0631930 MtrunA17_Chr1g0149831 38.333 180 81 3 68 220 1 177 9.98e-31 115
Msa0631930 MtrunA17_Chr6g0473731 30.220 182 109 3 89 257 10 186 9.22e-22 95.1
Msa0631930 MtrunA17_Chr8g0350761 23.481 362 203 9 13 307 20 374 2.17e-20 91.3
Msa0631930 MtrunA17_Chr7g0223971 28.169 213 97 5 91 254 1 206 1.65e-17 82.8
Msa0631930 MtrunA17_Chr2g0324891 39.560 91 53 2 136 224 127 217 5.14e-14 72.0
Msa0631930 MtrunA17_Chr5g0417521 47.826 69 31 2 168 231 15 83 1.01e-13 70.9
Msa0631930 MtrunA17_Chr7g0254981 39.796 98 56 2 159 255 41 136 1.76e-13 69.3
Msa0631930 MtrunA17_Chr7g0261911 43.011 93 48 3 159 249 253 342 2.74e-13 70.1
Msa0631930 MtrunA17_Chr6g0461641 55.556 54 23 1 168 220 42 95 5.45e-13 68.9
Msa0631930 MtrunA17_Chr4g0040481 46.552 58 31 0 167 224 157 214 6.57e-13 68.9
Msa0631930 MtrunA17_Chr5g0400241 34.940 83 54 0 167 249 310 392 1.37e-12 68.2
Msa0631930 MtrunA17_Chr4g0042451 51.852 54 25 1 168 220 17 70 1.58e-12 67.4
Msa0631930 MtrunA17_Chr2g0321461 53.704 54 24 1 168 220 45 98 2.14e-12 67.0
Msa0631930 MtrunA17_Chr2g0291021 57.407 54 22 1 168 220 49 102 5.92e-12 65.5
Msa0631930 MtrunA17_Chr7g0274471 47.222 72 36 2 168 237 218 289 9.66e-12 65.5
Msa0631930 MtrunA17_Chr8g0345901 28.226 124 85 2 12 131 34 157 1.04e-11 65.5
Msa0631930 MtrunA17_Chr7g0242521 54.286 70 23 4 155 220 5 69 1.13e-11 63.5
Msa0631930 MtrunA17_Chr4g0054981 50.000 54 27 0 167 220 309 362 1.16e-11 65.5
Msa0631930 MtrunA17_Chr7g0255711 48.276 58 30 0 167 224 90 147 2.41e-11 63.5
Msa0631930 MtrunA17_Chr1g0190301 51.724 58 27 1 164 220 171 228 2.65e-11 63.9
Msa0631930 MtrunA17_Chr1g0198601 46.575 73 31 2 149 220 199 264 3.04e-11 63.5
Msa0631930 MtrunA17_Chr4g0031961 46.552 58 31 0 167 224 130 187 3.54e-11 63.2
Msa0631930 MtrunA17_Chr7g0258551 50.000 56 27 1 166 220 210 265 5.82e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0631930 AT2G01760.1 38.039 255 119 5 13 230 10 262 4.22e-54 181
Msa0631930 AT1G67710.1 41.772 237 109 4 13 220 10 246 8.07e-54 184
Msa0631930 AT3G16857.1 38.976 254 109 6 13 220 36 289 1.54e-50 177
Msa0631930 AT3G16857.2 38.976 254 109 6 13 220 36 289 1.89e-50 177
Msa0631930 AT2G01760.2 34.276 283 119 6 13 230 10 290 4.02e-48 166
Msa0631930 AT4G31920.1 36.179 246 142 4 13 246 16 258 3.67e-47 166
Msa0631930 AT2G25180.1 36.638 232 123 3 13 220 16 247 5.81e-44 159
Msa0631930 AT5G58080.1 38.793 232 116 5 13 220 17 246 2.47e-40 149
Msa0631930 AT3G16857.3 37.615 218 90 6 49 220 2 219 5.49e-39 145
Msa0631930 AT2G01760.3 35.678 199 90 4 68 230 1 197 1.39e-36 133
Msa0631930 AT1G67710.2 41.436 181 78 3 68 220 1 181 9.09e-35 131
Msa0631930 AT5G49240.1 30.508 236 137 4 9 217 37 272 7.84e-31 117
Msa0631930 AT1G49190.1 26.074 326 194 9 16 303 36 352 1.89e-27 110
Msa0631930 AT3G62670.1 29.098 244 148 6 6 226 33 274 4.22e-27 110
Msa0631930 AT1G67710.3 39.752 161 69 3 88 220 12 172 4.14e-26 107
Msa0631930 AT5G07210.2 27.203 261 134 7 14 220 16 274 4.79e-24 102
Msa0631930 AT5G07210.1 26.394 269 134 8 14 220 16 282 1.66e-21 95.1
Msa0631930 AT2G27070.1 25.379 264 138 9 14 220 16 277 1.43e-18 86.7
Msa0631930 AT5G29000.1 34.161 161 90 5 103 254 123 276 2.09e-17 82.0
Msa0631930 AT5G29000.3 34.161 161 90 5 103 254 123 276 2.09e-17 82.0
Msa0631930 AT5G29000.2 34.161 161 90 5 103 254 166 319 2.58e-17 82.4
Msa0631930 AT5G29000.4 36.429 140 73 5 103 233 166 298 2.62e-17 81.3
Msa0631930 AT1G49190.2 31.933 119 80 1 16 133 36 154 7.81e-15 75.5
Msa0631930 AT2G01060.1 52.308 65 30 1 157 220 4 68 3.19e-14 72.0
Msa0631930 AT5G44190.1 51.724 58 28 0 167 224 146 203 1.17e-13 71.2
Msa0631930 AT5G44190.2 51.724 58 28 0 167 224 146 203 1.40e-13 70.9
Msa0631930 AT3G04030.1 55.556 54 23 1 168 220 45 98 4.23e-13 69.7
Msa0631930 AT3G04030.3 55.556 54 23 1 168 220 45 98 4.91e-13 69.3
Msa0631930 AT4G28610.1 46.067 89 44 3 168 254 225 311 5.97e-13 69.3
Msa0631930 AT5G18240.2 49.296 71 34 2 151 220 29 98 6.06e-13 69.3
Msa0631930 AT5G18240.3 49.296 71 34 2 151 220 29 98 6.06e-13 69.3
Msa0631930 AT5G18240.5 49.296 71 34 2 151 220 29 98 6.93e-13 68.9
Msa0631930 AT5G18240.1 49.296 71 34 2 151 220 29 98 6.97e-13 68.9
Msa0631930 AT5G18240.4 49.296 71 34 2 151 220 29 98 6.97e-13 68.9
Msa0631930 AT5G59570.2 43.056 72 38 1 149 220 125 193 2.33e-12 67.0
Msa0631930 AT5G59570.1 43.056 72 38 1 149 220 125 193 2.33e-12 67.0
Msa0631930 AT3G04450.1 44.737 76 35 2 168 242 238 307 2.48e-12 67.4
Msa0631930 AT3G04450.3 53.704 54 24 1 168 220 238 291 2.66e-12 66.6
Msa0631930 AT3G04030.2 52.830 53 25 0 168 220 45 97 2.78e-12 67.4
Msa0631930 AT5G60100.5 30.894 123 79 3 14 131 64 185 3.02e-12 67.4
Msa0631930 AT5G60100.3 30.894 123 79 3 14 131 64 185 3.02e-12 67.4
Msa0631930 AT5G60100.1 30.894 123 79 3 14 131 64 185 3.02e-12 67.4
Msa0631930 AT5G60100.2 30.894 123 79 3 14 131 64 185 3.04e-12 67.4
Msa0631930 AT5G60100.7 30.894 123 79 3 14 131 64 185 3.05e-12 67.0
Msa0631930 AT5G60100.6 30.894 123 79 3 14 131 64 185 3.05e-12 67.0
Msa0631930 AT5G60100.4 30.894 123 79 3 14 131 64 185 3.05e-12 67.0
Msa0631930 AT3G04450.4 44.737 76 35 2 168 242 198 267 3.34e-12 67.0
Msa0631930 AT3G04450.2 44.737 76 35 2 168 242 198 267 3.34e-12 67.0
Msa0631930 AT2G20570.1 48.276 58 30 0 167 224 152 209 4.32e-12 66.6
Msa0631930 AT1G69580.1 51.852 54 25 1 168 220 34 87 9.09e-12 65.5
Msa0631930 AT2G40970.1 50.000 54 27 0 167 220 104 157 9.13e-12 64.3
Msa0631930 AT1G69580.2 51.852 54 25 1 168 220 34 87 1.00e-11 65.5
Msa0631930 AT5G24470.1 27.778 126 81 3 13 131 49 171 1.09e-11 65.9
Msa0631930 AT5G05090.1 41.176 85 38 2 136 220 61 133 1.24e-11 64.3
Msa0631930 AT4G18020.8 38.158 76 44 1 150 225 281 353 1.45e-11 65.5
Msa0631930 AT4G18020.11 38.158 76 44 1 150 225 281 353 1.45e-11 65.5
Msa0631930 AT4G18020.4 38.158 76 44 1 150 225 281 353 1.45e-11 65.5
Msa0631930 AT4G18020.5 38.158 76 44 1 150 225 281 353 1.45e-11 65.5
Msa0631930 AT4G18020.10 38.158 76 44 1 150 225 281 353 1.45e-11 65.5
Msa0631930 AT4G18020.7 38.158 76 44 1 150 225 281 353 1.45e-11 65.5
Msa0631930 AT4G18020.3 38.158 76 44 1 150 225 281 353 1.70e-11 65.1
Msa0631930 AT4G18020.2 38.158 76 44 1 150 225 281 353 1.70e-11 65.1
Msa0631930 AT4G18020.1 38.158 76 44 1 150 225 281 353 1.70e-11 65.1
Msa0631930 AT4G18020.6 38.158 76 44 1 150 225 281 353 1.70e-11 65.1
Msa0631930 AT4G18020.9 38.158 76 44 1 150 225 281 353 1.70e-11 65.1
Msa0631930 AT3G10760.1 50.000 54 27 0 167 220 104 157 1.83e-11 64.3
Msa0631930 AT2G20570.2 49.091 55 28 0 170 224 171 225 3.03e-11 64.3
Msa0631930 AT3G46640.3 40.260 77 43 1 144 220 123 196 3.08e-11 63.9
Msa0631930 AT3G46640.2 48.148 54 28 0 167 220 143 196 3.33e-11 63.5
Msa0631930 AT3G46640.1 48.148 54 28 0 167 220 143 196 3.33e-11 63.5
Msa0631930 AT4G04605.1 37.624 101 51 3 166 264 12 102 4.66e-11 62.0
Msa0631930 AT5G06800.3 53.704 54 24 1 168 220 192 245 9.96e-11 62.0

Find 55 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGTTGCCAGCCATTTAC+AGG 0.283862 4_3:-15405950 Msa0631930:intron
GTGTAATCGATGCAATTATA+AGG 0.312923 4_3:-15406772 Msa0631930:CDS
ACATCTCCTCGACGAAATTA+AGG 0.321564 4_3:+15406793 None:intergenic
CAACAACATTGGGTGGAAAT+TGG 0.345706 4_3:-15405286 Msa0631930:CDS
TGAGTTGTAGGGTAACATTT+TGG 0.373812 4_3:+15406532 None:intergenic
GATAAATCATCATCAACATA+TGG 0.380393 4_3:+15405214 None:intergenic
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4_3:-15406356 Msa0631930:CDS
CGAATATTGGACTAAGCCTT+TGG 0.392176 4_3:-15406343 Msa0631930:CDS
ATCAGAAGCCATGCTACATT+TGG 0.418312 4_3:+15406641 None:intergenic
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4_3:+15406617 None:intergenic
TGGCAACATGTTATAAGAAA+AGG 0.422808 4_3:-15406297 Msa0631930:CDS
TGATGGTTTGTCGGTGAAAA+GGG 0.427097 4_3:+15406863 None:intergenic
AAAAGTGTTGCTCATAATGT+TGG 0.434928 4_3:+15405258 None:intergenic
CAGAATTATGAAGAAAACTT+TGG 0.439068 4_3:+15405338 None:intergenic
GTTTCAAGAAAATAGAAAAC+GGG 0.441425 4_3:-15406232 Msa0631930:CDS
TCAACATATGGGAACAAGTT+GGG 0.453000 4_3:+15405226 None:intergenic
ATGATGGTTTGTCGGTGAAA+AGG 0.455733 4_3:+15406862 None:intergenic
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4_3:-15406616 Msa0631930:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4_3:+15406504 None:intergenic
TTCTCGCGACCAAGACAAAC+GGG 0.470376 4_3:+15406164 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_3:-15406173 Msa0631930:CDS
TGTAATCGATGCAATTATAA+GGG 0.476930 4_3:-15406771 Msa0631930:intron
TTGCGATGCACAACAACATT+GGG 0.477561 4_3:-15405296 Msa0631930:CDS
AAAAGAAACACATGCAAAGA+AGG 0.478150 4_3:-15406187 Msa0631930:CDS
TTGAGACCTTCTGATAATGA+TGG 0.479264 4_3:+15406846 None:intergenic
TACTCACTCAATACCGGAGC+AGG 0.481604 4_3:-15405362 Msa0631930:CDS
AAGTCATAAACATCCATGTC+TGG 0.491105 4_3:+15406558 None:intergenic
TTCTGATAATGATGGTTTGT+CGG 0.493949 4_3:+15406854 None:intergenic
ATATGAAATTGTTGGAAGCT+CGG 0.504899 4_3:-15406256 Msa0631930:CDS
TGTTTCAAGAAAATAGAAAA+CGG 0.506234 4_3:-15406233 Msa0631930:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4_3:+15406103 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4_3:-15406372 Msa0631930:CDS
AAGCTAAATTGTGTTTCAAG+AGG 0.513615 4_3:+15405391 None:intergenic
AGAAAACTTTGGTCCTGCTC+CGG 0.527471 4_3:+15405349 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4_3:-15406625 Msa0631930:CDS
TTATGAAGTCTATTGAATGT+GGG 0.532174 4_3:-15406371 Msa0631930:CDS
AAAACGAACCTGTAAATGGC+TGG 0.537566 4_3:+15405942 None:intergenic
TTCAGTTACCAAATGTAGCA+TGG 0.538628 4_3:-15406649 Msa0631930:intron
AGCTCAACCGATGAGACAAA+AGG 0.540383 4_3:-15405439 Msa0631930:CDS
ATTCTCGCGACCAAGACAAA+CGG 0.543227 4_3:+15406163 None:intergenic
CTACGTGCAGTCAATCAACT+AGG 0.547867 4_3:-15406126 Msa0631930:CDS
ATTGCGATGCACAACAACAT+TGG 0.547991 4_3:-15405297 Msa0631930:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4_3:+15405215 None:intergenic
ATCAACATATGGGAACAAGT+TGG 0.552596 4_3:+15405225 None:intergenic
AGAAGCTACTCACTCAATAC+CGG 0.567403 4_3:-15405368 Msa0631930:CDS
ATCATTATCAGAAGGTCTCA+AGG 0.582734 4_3:-15406844 Msa0631930:CDS
CGATGCACAACAACATTGGG+TGG 0.592869 4_3:-15405293 Msa0631930:CDS
AGAACTTTCTGCAGAATCAA+CGG 0.593830 4_3:+15406395 None:intergenic
TCTTATAACATGTTGCCACA+TGG 0.594344 4_3:+15406302 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.598969 4_3:-15406370 Msa0631930:CDS
CACTCTCCTTAATTTCGTCG+AGG 0.625060 4_3:-15406799 Msa0631930:CDS
TGATGCTCATATGCCAGACA+TGG 0.629107 4_3:-15406571 Msa0631930:CDS
GACAAACCATCATTATCAGA+AGG 0.636966 4_3:-15406852 Msa0631930:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4_3:-15405201 Msa0631930:CDS
TTTCAAGAAAATAGAAAACG+GGG 0.736712 4_3:-15406231 Msa0631930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAAATATGAAATTGT+TGG - chr4_3:15405801-15405820 Msa0631930:intron 15.0%
!!! ATAATTTTCAGAATCTTTTT+TGG + chr4_3:15406071-15406090 None:intergenic 15.0%
!!! AAAAAAGATTCTGAAAATTA+TGG - chr4_3:15406070-15406089 Msa0631930:intron 15.0%
!! TTATTGTAAACTGAAATTTA+TGG - chr4_3:15406537-15406556 Msa0631930:CDS 15.0%
!! AACAAAAAATATGAAATTGT+TGG - chr4_3:15405801-15405820 Msa0631930:intron 15.0%
!!! ATAATTTTCAGAATCTTTTT+TGG + chr4_3:15406071-15406090 None:intergenic 15.0%
!!! AAAAAAGATTCTGAAAATTA+TGG - chr4_3:15406070-15406089 Msa0631930:intron 15.0%
!! TTATTGTAAACTGAAATTTA+TGG - chr4_3:15406537-15406556 Msa0631930:CDS 15.0%
!! AAAGATGAAAACTTTGAATA+AGG + chr4_3:15405337-15405356 None:intergenic 20.0%
!!! TTCAAAGTTTTCATCTTTTT+TGG - chr4_3:15405339-15405358 Msa0631930:CDS 20.0%
!! AATAAATTCTTACTAATGAC+AGG + chr4_3:15405564-15405583 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG - chr4_3:15405647-15405666 Msa0631930:intron 20.0%
!! TGTTTCAAGAAAATAGAAAA+CGG - chr4_3:15405832-15405851 Msa0631930:intron 20.0%
!! AGCATAGTAAAAAAGTAATT+AGG + chr4_3:15405985-15406004 None:intergenic 20.0%
!!! ATGTTTTTCTCTTATTTTTC+AGG - chr4_3:15406038-15406057 Msa0631930:intron 20.0%
!!! TGTTTTTCTCTTATTTTTCA+GGG - chr4_3:15406039-15406058 Msa0631930:intron 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG + chr4_3:15406102-15406121 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG + chr4_3:15406853-15406872 None:intergenic 20.0%
!! AAAGATGAAAACTTTGAATA+AGG + chr4_3:15405337-15405356 None:intergenic 20.0%
!!! TTCAAAGTTTTCATCTTTTT+TGG - chr4_3:15405339-15405358 Msa0631930:CDS 20.0%
!! AATAAATTCTTACTAATGAC+AGG + chr4_3:15405564-15405583 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG - chr4_3:15405647-15405666 Msa0631930:intron 20.0%
!! TGTTTCAAGAAAATAGAAAA+CGG - chr4_3:15405832-15405851 Msa0631930:intron 20.0%
!! AGCATAGTAAAAAAGTAATT+AGG + chr4_3:15405985-15406004 None:intergenic 20.0%
!!! ATGTTTTTCTCTTATTTTTC+AGG - chr4_3:15406038-15406057 Msa0631930:intron 20.0%
!!! TGTTTTTCTCTTATTTTTCA+GGG - chr4_3:15406039-15406058 Msa0631930:intron 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG + chr4_3:15406102-15406121 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG + chr4_3:15406853-15406872 None:intergenic 20.0%
! TGTAATCGATGCAATTATAA+GGG - chr4_3:15405294-15405313 Msa0631930:CDS 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4_3:15405693-15405712 Msa0631930:intron 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4_3:15405694-15405713 Msa0631930:intron 25.0%
! GTTTCAAGAAAATAGAAAAC+GGG - chr4_3:15405833-15405852 Msa0631930:intron 25.0%
! TTTCAAGAAAATAGAAAACG+GGG - chr4_3:15405834-15405853 Msa0631930:intron 25.0%
!!! GTTTTTCTCTTATTTTTCAG+GGG - chr4_3:15406040-15406059 Msa0631930:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG + chr4_3:15406101-15406120 None:intergenic 25.0%
! ATGCATAAGCTATTAAATTC+AGG + chr4_3:15406358-15406377 None:intergenic 25.0%
! TAACAGCTAGAAAAAGAATT+TGG + chr4_3:15406392-15406411 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG + chr4_3:15406501-15406520 None:intergenic 25.0%
! CAGAATTATGAAGAAAACTT+TGG + chr4_3:15406730-15406749 None:intergenic 25.0%
! GATAAATCATCATCAACATA+TGG + chr4_3:15406854-15406873 None:intergenic 25.0%
! TGTAATCGATGCAATTATAA+GGG - chr4_3:15405294-15405313 Msa0631930:CDS 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4_3:15405693-15405712 Msa0631930:intron 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4_3:15405694-15405713 Msa0631930:intron 25.0%
! GTTTCAAGAAAATAGAAAAC+GGG - chr4_3:15405833-15405852 Msa0631930:intron 25.0%
! TTTCAAGAAAATAGAAAACG+GGG - chr4_3:15405834-15405853 Msa0631930:intron 25.0%
!!! GTTTTTCTCTTATTTTTCAG+GGG - chr4_3:15406040-15406059 Msa0631930:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG + chr4_3:15406101-15406120 None:intergenic 25.0%
! ATGCATAAGCTATTAAATTC+AGG + chr4_3:15406358-15406377 None:intergenic 25.0%
! TAACAGCTAGAAAAAGAATT+TGG + chr4_3:15406392-15406411 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG + chr4_3:15406501-15406520 None:intergenic 25.0%
! CAGAATTATGAAGAAAACTT+TGG + chr4_3:15406730-15406749 None:intergenic 25.0%
! GATAAATCATCATCAACATA+TGG + chr4_3:15406854-15406873 None:intergenic 25.0%
! TTCTGATAATGATGGTTTGT+CGG + chr4_3:15405214-15405233 None:intergenic 30.0%
GTGTAATCGATGCAATTATA+AGG - chr4_3:15405293-15405312 Msa0631930:CDS 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4_3:15405695-15405714 Msa0631930:intron 30.0%
!! TTGAACTGTTTTTCAACCAA+AGG + chr4_3:15405741-15405760 None:intergenic 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4_3:15405768-15405787 Msa0631930:intron 30.0%
ATATGAAATTGTTGGAAGCT+CGG - chr4_3:15405809-15405828 Msa0631930:intron 30.0%
AAAAGAAACACATGCAAAGA+AGG - chr4_3:15405878-15405897 Msa0631930:intron 30.0%
AGGAAAGAATCATACTATCA+AGG + chr4_3:15405965-15405984 None:intergenic 30.0%
TACAAAAACGAACCTGTAAA+TGG + chr4_3:15406130-15406149 None:intergenic 30.0%
AGAATTACAACAACAGACTT+AGG + chr4_3:15406227-15406246 None:intergenic 30.0%
TTGTATAACATGCAAGACTA+AGG + chr4_3:15406273-15406292 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+CGG + chr4_3:15406483-15406502 None:intergenic 30.0%
AAGCTAAATTGTGTTTCAAG+AGG + chr4_3:15406677-15406696 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG + chr4_3:15406810-15406829 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG - chr4_3:15406864-15406883 Msa0631930:CDS 30.0%
! TTCTGATAATGATGGTTTGT+CGG + chr4_3:15405214-15405233 None:intergenic 30.0%
GTGTAATCGATGCAATTATA+AGG - chr4_3:15405293-15405312 Msa0631930:CDS 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4_3:15405695-15405714 Msa0631930:intron 30.0%
!! TTGAACTGTTTTTCAACCAA+AGG + chr4_3:15405741-15405760 None:intergenic 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4_3:15405768-15405787 Msa0631930:intron 30.0%
ATATGAAATTGTTGGAAGCT+CGG - chr4_3:15405809-15405828 Msa0631930:intron 30.0%
AAAAGAAACACATGCAAAGA+AGG - chr4_3:15405878-15405897 Msa0631930:intron 30.0%
AGGAAAGAATCATACTATCA+AGG + chr4_3:15405965-15405984 None:intergenic 30.0%
TACAAAAACGAACCTGTAAA+TGG + chr4_3:15406130-15406149 None:intergenic 30.0%
AGAATTACAACAACAGACTT+AGG + chr4_3:15406227-15406246 None:intergenic 30.0%
TTGTATAACATGCAAGACTA+AGG + chr4_3:15406273-15406292 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+CGG + chr4_3:15406483-15406502 None:intergenic 30.0%
AAGCTAAATTGTGTTTCAAG+AGG + chr4_3:15406677-15406696 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG + chr4_3:15406810-15406829 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG - chr4_3:15406864-15406883 Msa0631930:CDS 30.0%
GACAAACCATCATTATCAGA+AGG - chr4_3:15405213-15405232 Msa0631930:CDS 35.0%
! TTGAGACCTTCTGATAATGA+TGG + chr4_3:15405222-15405241 None:intergenic 35.0%
ATCATTATCAGAAGGTCTCA+AGG - chr4_3:15405221-15405240 Msa0631930:CDS 35.0%
TTCAGTTACCAAATGTAGCA+TGG - chr4_3:15405416-15405435 Msa0631930:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG + chr4_3:15405451-15405470 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG - chr4_3:15405449-15405468 Msa0631930:CDS 35.0%
AAGTCATAAACATCCATGTC+TGG + chr4_3:15405510-15405529 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG + chr4_3:15405536-15405555 None:intergenic 35.0%
!!! GACAGGAATTTTGAGTTGTA+GGG + chr4_3:15405547-15405566 None:intergenic 35.0%
!!! TGACAGGAATTTTGAGTTGT+AGG + chr4_3:15405548-15405567 None:intergenic 35.0%
AGAACTTTCTGCAGAATCAA+CGG + chr4_3:15405673-15405692 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG - chr4_3:15405748-15405767 Msa0631930:intron 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4_3:15405766-15405785 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG + chr4_3:15405877-15405896 None:intergenic 35.0%
AAAAGATCACTGACAAACCT+AGG + chr4_3:15406203-15406222 None:intergenic 35.0%
! CATCTTACCTTTTGTCTCAT+CGG + chr4_3:15406636-15406655 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG + chr4_3:15406842-15406861 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG + chr4_3:15406843-15406862 None:intergenic 35.0%
GACAAACCATCATTATCAGA+AGG - chr4_3:15405213-15405232 Msa0631930:CDS 35.0%
! TTGAGACCTTCTGATAATGA+TGG + chr4_3:15405222-15405241 None:intergenic 35.0%
ATCATTATCAGAAGGTCTCA+AGG - chr4_3:15405221-15405240 Msa0631930:CDS 35.0%
TTCAGTTACCAAATGTAGCA+TGG - chr4_3:15405416-15405435 Msa0631930:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG + chr4_3:15405451-15405470 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG - chr4_3:15405449-15405468 Msa0631930:CDS 35.0%
AAGTCATAAACATCCATGTC+TGG + chr4_3:15405510-15405529 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG + chr4_3:15405536-15405555 None:intergenic 35.0%
!!! GACAGGAATTTTGAGTTGTA+GGG + chr4_3:15405547-15405566 None:intergenic 35.0%
!!! TGACAGGAATTTTGAGTTGT+AGG + chr4_3:15405548-15405567 None:intergenic 35.0%
AGAACTTTCTGCAGAATCAA+CGG + chr4_3:15405673-15405692 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG - chr4_3:15405748-15405767 Msa0631930:intron 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4_3:15405766-15405785 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG + chr4_3:15405877-15405896 None:intergenic 35.0%
AAAAGATCACTGACAAACCT+AGG + chr4_3:15406203-15406222 None:intergenic 35.0%
! CATCTTACCTTTTGTCTCAT+CGG + chr4_3:15406636-15406655 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG + chr4_3:15406842-15406861 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG + chr4_3:15406843-15406862 None:intergenic 35.0%
! TGATGGTTTGTCGGTGAAAA+GGG + chr4_3:15405205-15405224 None:intergenic 40.0%
! ATGATGGTTTGTCGGTGAAA+AGG + chr4_3:15405206-15405225 None:intergenic 40.0%
ACATCTCCTCGACGAAATTA+AGG + chr4_3:15405275-15405294 None:intergenic 40.0%
ATCAGAAGCCATGCTACATT+TGG + chr4_3:15405427-15405446 None:intergenic 40.0%
TTCTGATGCCTTCAACATGT+TGG - chr4_3:15405440-15405459 Msa0631930:CDS 40.0%
CGAATATTGGACTAAGCCTT+TGG - chr4_3:15405722-15405741 Msa0631930:intron 40.0%
AAAACGAACCTGTAAATGGC+TGG + chr4_3:15406126-15406145 None:intergenic 40.0%
AAACCTAGGCAACTAGACTT+AGG + chr4_3:15406189-15406208 None:intergenic 40.0%
GAGTAGCTTCAGCTACTTAT+AGG - chr4_3:15406313-15406332 Msa0631930:CDS 40.0%
CAGGTTCGTTTCATTTACAC+CGG + chr4_3:15406339-15406358 None:intergenic 40.0%
AGAAGCTACTCACTCAATAC+CGG - chr4_3:15406697-15406716 Msa0631930:intron 40.0%
ATTGCGATGCACAACAACAT+TGG - chr4_3:15406768-15406787 Msa0631930:intron 40.0%
TTGCGATGCACAACAACATT+GGG - chr4_3:15406769-15406788 Msa0631930:intron 40.0%
CAACAACATTGGGTGGAAAT+TGG - chr4_3:15406779-15406798 Msa0631930:CDS 40.0%
! TGATGGTTTGTCGGTGAAAA+GGG + chr4_3:15405205-15405224 None:intergenic 40.0%
! ATGATGGTTTGTCGGTGAAA+AGG + chr4_3:15405206-15405225 None:intergenic 40.0%
ACATCTCCTCGACGAAATTA+AGG + chr4_3:15405275-15405294 None:intergenic 40.0%
ATCAGAAGCCATGCTACATT+TGG + chr4_3:15405427-15405446 None:intergenic 40.0%
TTCTGATGCCTTCAACATGT+TGG - chr4_3:15405440-15405459 Msa0631930:CDS 40.0%
CGAATATTGGACTAAGCCTT+TGG - chr4_3:15405722-15405741 Msa0631930:intron 40.0%
AAAACGAACCTGTAAATGGC+TGG + chr4_3:15406126-15406145 None:intergenic 40.0%
AAACCTAGGCAACTAGACTT+AGG + chr4_3:15406189-15406208 None:intergenic 40.0%
GAGTAGCTTCAGCTACTTAT+AGG - chr4_3:15406313-15406332 Msa0631930:CDS 40.0%
CAGGTTCGTTTCATTTACAC+CGG + chr4_3:15406339-15406358 None:intergenic 40.0%
AGAAGCTACTCACTCAATAC+CGG - chr4_3:15406697-15406716 Msa0631930:intron 40.0%
ATTGCGATGCACAACAACAT+TGG - chr4_3:15406768-15406787 Msa0631930:intron 40.0%
TTGCGATGCACAACAACATT+GGG - chr4_3:15406769-15406788 Msa0631930:intron 40.0%
CAACAACATTGGGTGGAAAT+TGG - chr4_3:15406779-15406798 Msa0631930:CDS 40.0%
CACTCTCCTTAATTTCGTCG+AGG - chr4_3:15405266-15405285 Msa0631930:CDS 45.0%
TGATGCTCATATGCCAGACA+TGG - chr4_3:15405494-15405513 Msa0631930:intron 45.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4_3:15405709-15405728 Msa0631930:intron 45.0%
ATTCTCGCGACCAAGACAAA+CGG + chr4_3:15405905-15405924 None:intergenic 45.0%
CTACGTGCAGTCAATCAACT+AGG - chr4_3:15405939-15405958 Msa0631930:intron 45.0%
ACATGTTGCCAGCCATTTAC+AGG - chr4_3:15406115-15406134 Msa0631930:intron 45.0%
GTACCTAAGTCTAGTTGCCT+AGG - chr4_3:15406183-15406202 Msa0631930:CDS 45.0%
AGCTTCAGCTACTTATAGGC+CGG - chr4_3:15406317-15406336 Msa0631930:CDS 45.0%
AGCTCAACCGATGAGACAAA+AGG - chr4_3:15406626-15406645 Msa0631930:CDS 45.0%
AGAAAACTTTGGTCCTGCTC+CGG + chr4_3:15406719-15406738 None:intergenic 45.0%
CACTCTCCTTAATTTCGTCG+AGG - chr4_3:15405266-15405285 Msa0631930:CDS 45.0%
TGATGCTCATATGCCAGACA+TGG - chr4_3:15405494-15405513 Msa0631930:intron 45.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4_3:15405709-15405728 Msa0631930:intron 45.0%
ATTCTCGCGACCAAGACAAA+CGG + chr4_3:15405905-15405924 None:intergenic 45.0%
CTACGTGCAGTCAATCAACT+AGG - chr4_3:15405939-15405958 Msa0631930:intron 45.0%
ACATGTTGCCAGCCATTTAC+AGG - chr4_3:15406115-15406134 Msa0631930:intron 45.0%
GTACCTAAGTCTAGTTGCCT+AGG - chr4_3:15406183-15406202 Msa0631930:CDS 45.0%
AGCTTCAGCTACTTATAGGC+CGG - chr4_3:15406317-15406336 Msa0631930:CDS 45.0%
AGCTCAACCGATGAGACAAA+AGG - chr4_3:15406626-15406645 Msa0631930:CDS 45.0%
AGAAAACTTTGGTCCTGCTC+CGG + chr4_3:15406719-15406738 None:intergenic 45.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4_3:15405892-15405911 Msa0631930:intron 50.0%
TTCTCGCGACCAAGACAAAC+GGG + chr4_3:15405904-15405923 None:intergenic 50.0%
TACTCACTCAATACCGGAGC+AGG - chr4_3:15406703-15406722 Msa0631930:intron 50.0%
CGATGCACAACAACATTGGG+TGG - chr4_3:15406772-15406791 Msa0631930:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4_3:15405892-15405911 Msa0631930:intron 50.0%
TTCTCGCGACCAAGACAAAC+GGG + chr4_3:15405904-15405923 None:intergenic 50.0%
TACTCACTCAATACCGGAGC+AGG - chr4_3:15406703-15406722 Msa0631930:intron 50.0%
CGATGCACAACAACATTGGG+TGG - chr4_3:15406772-15406791 Msa0631930:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 15405198 15406889 15405198 ID=Msa0631930;Name=Msa0631930
chr4_3 mRNA 15405198 15406889 15405198 ID=Msa0631930-mRNA-1;Parent=Msa0631930;Name=Msa0631930-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|0.8|5|0|311
chr4_3 exon 15406772 15406889 15406772 ID=Msa0631930-mRNA-1:exon:4406;Parent=Msa0631930-mRNA-1
chr4_3 exon 15406517 15406666 15406517 ID=Msa0631930-mRNA-1:exon:4405;Parent=Msa0631930-mRNA-1
chr4_3 exon 15406118 15406423 15406118 ID=Msa0631930-mRNA-1:exon:4404;Parent=Msa0631930-mRNA-1
chr4_3 exon 15405951 15406027 15405951 ID=Msa0631930-mRNA-1:exon:4403;Parent=Msa0631930-mRNA-1
chr4_3 exon 15405198 15405482 15405198 ID=Msa0631930-mRNA-1:exon:4402;Parent=Msa0631930-mRNA-1
chr4_3 CDS 15406772 15406889 15406772 ID=Msa0631930-mRNA-1:cds;Parent=Msa0631930-mRNA-1
chr4_3 CDS 15406517 15406666 15406517 ID=Msa0631930-mRNA-1:cds;Parent=Msa0631930-mRNA-1
chr4_3 CDS 15406118 15406423 15406118 ID=Msa0631930-mRNA-1:cds;Parent=Msa0631930-mRNA-1
chr4_3 CDS 15405951 15406027 15405951 ID=Msa0631930-mRNA-1:cds;Parent=Msa0631930-mRNA-1
chr4_3 CDS 15405198 15405482 15405198 ID=Msa0631930-mRNA-1:cds;Parent=Msa0631930-mRNA-1
Gene Sequence

>Msa0631930

ATGGCCCTTTTCACCGACAAACCATCATTATCAGAAGGTCTCAAGGTTCTTGCAATTGATCATGACATCACTCTCCTTAATTTCGTCGAGGAGATGTGTAATCGATGCAATTATAAGGTTACCAAATGTAGCATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAAAATTCCTGTCATTATGATGGCCGTTGATTCTGCAGAAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAACAGTTCAAGACCATGTGGCAACATGTTATAAGAAAAGGTTTGACTCAAAACAAAAAATATGAAATTGTTGGAAGCTCGGTGTTTCAAGAAAATAGAAAACGGGGCAGAGAAGATGCTAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATTATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGAAGTATAGAAATCAGTTTAAGAGCTCAACCGATGAGACAAAAGGTAAGATGAAAAAATCAAAGAAGAGTCCTCTTGAAACACAATTTAGCTTAGAAGCTACTCACTCAATACCGGAGCAGGACCAAAGTTTTCTTCATAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACAACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCTGACTTTCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>Msa0631930

MALFTDKPSLSEGLKVLAIDHDITLLNFVEEMCNRCNYKVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLKIPVIMMAVDSAESSIMKSIECGACEYWTKPLVEKQFKTMWQHVIRKGLTQNKKYEIVGSSVFQENRKRGREDANASKETHAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHVASHLQKYRNQFKSSTDETKGKMKKSKKSPLETQFSLEATHSIPEQDQSFLHNSATQCRDNCDAQQHWVEIGESNIMSNTFSDFPNLFPYVDDDLSSLVW*