AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0632340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0632340 MtrunA17_Chr4g0009851 82.166 314 53 2 1 313 1 312 0.0 538
Msa0632340 MtrunA17_Chr4g0009981 85.441 261 36 1 53 313 1 259 2.45e-168 468
Msa0632340 MtrunA17_Chr4g0009901 78.099 242 51 1 1 242 1 240 1.33e-134 383
Msa0632340 MtrunA17_Chr4g0011071 79.137 139 27 1 55 193 35 171 1.63e-76 231
Msa0632340 MtrunA17_Chr1g0161431 45.565 248 102 5 10 226 11 256 1.39e-66 219
Msa0632340 MtrunA17_Chr6g0471041 43.464 306 150 9 1 296 1 293 7.88e-66 212
Msa0632340 MtrunA17_Chr4g0009991 77.311 119 24 2 101 219 1 116 3.95e-57 180
Msa0632340 MtrunA17_Chr8g0376881 40.856 257 122 5 9 239 24 276 4.08e-56 190
Msa0632340 MtrunA17_Chr3g0123131 40.984 244 114 4 10 225 13 254 6.42e-56 190
Msa0632340 MtrunA17_Chr4g0067981 40.741 243 116 3 10 226 31 271 5.04e-55 189
Msa0632340 MtrunA17_Chr2g0294911 39.098 266 134 4 10 249 29 292 8.82e-55 187
Msa0632340 MtrunA17_Chr4g0010001 87.640 89 11 0 1 89 1 89 7.73e-54 171
Msa0632340 MtrunA17_Chr1g0149841 39.535 258 128 5 7 236 2 259 4.16e-51 176
Msa0632340 MtrunA17_Chr5g0423031 85.393 89 13 0 1 89 1 89 4.71e-50 164
Msa0632340 MtrunA17_Chr8g0391911 38.430 242 121 4 4 219 3 242 1.00e-48 163
Msa0632340 MtrunA17_Chr7g0275401 37.109 256 128 4 7 232 2 254 1.31e-47 161
Msa0632340 MtrunA17_Chr3g0137101 37.247 247 125 3 10 226 8 254 1.37e-46 166
Msa0632340 MtrunA17_Chr7g0275891 35.018 277 139 5 7 242 2 278 9.90e-46 158
Msa0632340 MtrunA17_Chr2g0304841 34.902 255 131 4 10 229 18 272 1.78e-44 160
Msa0632340 MtrunA17_Chr8g0383961 40.794 277 122 11 1 265 1 247 9.09e-44 150
Msa0632340 MtrunA17_Chr4g0074401 33.068 251 140 4 10 233 17 266 6.59e-42 152
Msa0632340 MtrunA17_Chr1g0149851 35.632 261 127 5 7 226 2 262 7.49e-42 152
Msa0632340 MtrunA17_Chr4g0074411 34.137 249 139 4 9 233 17 264 1.37e-41 152
Msa0632340 MtrunA17_Chr6g0456631 41.875 160 89 2 4 161 3 160 3.73e-39 135
Msa0632340 MtrunA17_Chr3g0134461 36.792 212 105 4 10 219 8 192 2.88e-37 131
Msa0632340 MtrunA17_Chr3g0134481 34.703 219 103 4 4 221 4 183 7.01e-36 127
Msa0632340 MtrunA17_Chr4g0074421 35.714 182 95 3 59 219 2 182 6.23e-35 125
Msa0632340 MtrunA17_Chr1g0149831 36.735 196 94 4 68 236 1 193 2.03e-31 117
Msa0632340 MtrunA17_Chr6g0473731 31.111 180 112 3 89 259 10 186 1.75e-20 91.3
Msa0632340 MtrunA17_Chr2g0324891 42.857 91 50 2 138 226 127 217 4.76e-16 78.2
Msa0632340 MtrunA17_Chr5g0417521 59.259 54 21 1 170 222 15 68 1.19e-15 76.6
Msa0632340 MtrunA17_Chr6g0461641 59.259 54 21 1 170 222 42 95 5.93e-15 74.7
Msa0632340 MtrunA17_Chr4g0040481 50.000 58 29 0 169 226 157 214 1.10e-14 74.3
Msa0632340 MtrunA17_Chr7g0255711 55.172 58 26 0 169 226 90 147 1.27e-14 73.2
Msa0632340 MtrunA17_Chr4g0031961 53.448 58 27 0 169 226 130 187 1.75e-14 72.8
Msa0632340 MtrunA17_Chr2g0321461 57.407 54 22 1 170 222 45 98 2.33e-14 73.2
Msa0632340 MtrunA17_Chr7g0254981 41.837 98 54 2 161 257 41 136 4.22e-14 71.2
Msa0632340 MtrunA17_Chr7g0261911 50.649 77 35 3 170 245 262 336 2.02e-13 70.5
Msa0632340 MtrunA17_Chr4g0054981 37.349 83 52 0 169 251 309 391 3.16e-13 70.1
Msa0632340 MtrunA17_Chr5g0400241 37.349 83 52 0 169 251 310 392 3.96e-13 69.7
Msa0632340 MtrunA17_Chr4g0042451 53.704 54 24 1 170 222 17 70 6.22e-13 68.6
Msa0632340 MtrunA17_Chr7g0274471 53.846 65 27 2 170 231 218 282 1.11e-12 68.2
Msa0632340 MtrunA17_Chr2g0291021 56.522 69 25 3 170 233 49 117 1.56e-12 67.4
Msa0632340 MtrunA17_Chr1g0190301 53.448 58 26 1 166 222 171 228 2.82e-12 66.6
Msa0632340 MtrunA17_Chr8g0356501 42.697 89 49 2 170 256 37 125 1.01e-11 65.1
Msa0632340 MtrunA17_Chr1g0162931 41.772 79 44 2 156 233 5 82 1.31e-11 63.9
Msa0632340 MtrunA17_Chr7g0243081 51.786 56 26 1 168 222 69 124 2.10e-11 63.9
Msa0632340 MtrunA17_Chr7g0242521 54.286 70 23 4 157 222 5 69 2.76e-11 62.4
Msa0632340 MtrunA17_Chr3g0095611 49.123 57 28 1 168 223 17 73 2.95e-11 62.0
Msa0632340 MtrunA17_Chr6g0479431 47.541 61 31 1 163 222 195 255 6.02e-11 62.8
Msa0632340 MtrunA17_Chr1g0198601 46.575 73 31 2 151 222 199 264 9.13e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0632340 AT2G01760.1 38.931 262 119 6 7 231 4 261 8.78e-57 188
Msa0632340 AT1G67710.1 39.623 265 128 6 11 243 8 272 2.62e-52 180
Msa0632340 AT2G01760.2 35.172 290 119 7 7 231 4 289 8.49e-51 173
Msa0632340 AT3G16857.1 37.319 276 127 7 10 239 33 308 2.83e-50 177
Msa0632340 AT3G16857.2 37.319 276 127 7 10 239 33 308 3.46e-50 177
Msa0632340 AT4G31920.1 35.743 249 147 3 10 248 13 258 5.99e-47 166
Msa0632340 AT2G25180.1 38.272 243 120 4 10 226 13 251 1.37e-45 163
Msa0632340 AT5G58080.1 38.000 250 129 6 10 236 14 260 7.51e-40 147
Msa0632340 AT3G16857.3 35.865 237 106 7 49 239 2 238 1.84e-38 144
Msa0632340 AT2G01760.3 36.500 200 87 5 68 231 1 196 2.37e-36 133
Msa0632340 AT1G67710.2 38.647 207 96 5 68 243 1 207 7.04e-35 132
Msa0632340 AT5G49240.1 31.356 236 137 5 9 219 37 272 1.98e-33 124
Msa0632340 AT3G62670.1 31.019 216 128 5 28 222 53 268 1.04e-29 117
Msa0632340 AT1G49190.1 28.926 242 143 5 10 222 30 271 3.19e-29 115
Msa0632340 AT1G67710.3 37.433 187 86 5 88 243 12 198 2.85e-26 108
Msa0632340 AT5G07210.2 26.667 270 144 6 14 231 16 283 1.68e-25 107
Msa0632340 AT5G07210.1 25.899 278 144 7 14 231 16 291 5.09e-23 99.8
Msa0632340 AT2G27070.1 24.101 278 154 7 14 236 16 291 7.59e-19 87.4
Msa0632340 AT1G49190.2 32.258 124 83 1 10 132 30 153 1.44e-17 83.6
Msa0632340 AT5G29000.4 39.669 121 62 5 120 238 190 301 9.18e-16 77.0
Msa0632340 AT2G01060.1 50.602 83 38 2 159 238 4 86 9.38e-16 76.6
Msa0632340 AT5G29000.1 33.728 169 88 7 103 256 123 282 1.28e-15 77.0
Msa0632340 AT5G29000.3 33.728 169 88 7 103 256 123 282 1.28e-15 77.0
Msa0632340 AT5G59570.2 49.296 71 33 1 156 226 130 197 1.35e-15 76.3
Msa0632340 AT5G59570.1 49.296 71 33 1 156 226 130 197 1.35e-15 76.3
Msa0632340 AT5G29000.2 34.868 152 81 6 103 245 166 308 1.44e-15 77.0
Msa0632340 AT5G05090.1 44.944 89 37 2 138 226 61 137 3.96e-15 74.3
Msa0632340 AT3G04030.1 59.259 54 21 1 170 222 45 98 6.59e-15 75.1
Msa0632340 AT2G40970.1 53.448 58 27 0 169 226 104 161 7.19e-15 73.6
Msa0632340 AT3G04030.3 59.259 54 21 1 170 222 45 98 7.57e-15 75.1
Msa0632340 AT3G10760.1 55.172 58 26 0 169 226 104 161 9.08e-15 74.3
Msa0632340 AT5G44190.1 53.448 58 27 0 169 226 146 203 9.15e-15 74.7
Msa0632340 AT5G18240.2 52.113 71 32 2 153 222 29 98 1.06e-14 74.3
Msa0632340 AT5G18240.3 52.113 71 32 2 153 222 29 98 1.06e-14 74.3
Msa0632340 AT5G44190.2 53.448 58 27 0 169 226 146 203 1.12e-14 74.3
Msa0632340 AT5G18240.5 52.113 71 32 2 153 222 29 98 1.17e-14 74.3
Msa0632340 AT5G18240.1 52.113 71 32 2 153 222 29 98 1.19e-14 74.3
Msa0632340 AT5G18240.4 52.113 71 32 2 153 222 29 98 1.19e-14 74.3
Msa0632340 AT3G46640.3 53.448 58 27 0 169 226 143 200 1.80e-14 73.2
Msa0632340 AT3G46640.2 53.448 58 27 0 169 226 143 200 1.99e-14 73.2
Msa0632340 AT3G46640.1 53.448 58 27 0 169 226 143 200 1.99e-14 73.2
Msa0632340 AT3G04030.2 56.604 53 23 0 170 222 45 97 4.40e-14 72.8
Msa0632340 AT2G20570.1 51.724 58 28 0 169 226 152 209 8.11e-14 72.0
Msa0632340 AT4G28610.1 61.111 54 20 1 170 222 225 278 3.71e-13 70.1
Msa0632340 AT3G04450.3 55.556 54 23 1 170 222 238 291 4.09e-13 69.3
Msa0632340 AT2G20570.2 52.727 55 26 0 172 226 171 225 5.55e-13 69.7
Msa0632340 AT3G04450.1 55.556 54 23 1 170 222 238 291 6.05e-13 69.3
Msa0632340 AT4G18020.8 39.474 76 43 1 152 227 281 353 6.33e-13 69.3
Msa0632340 AT4G18020.11 39.474 76 43 1 152 227 281 353 6.33e-13 69.3
Msa0632340 AT4G18020.4 39.474 76 43 1 152 227 281 353 6.33e-13 69.3
Msa0632340 AT4G18020.5 39.474 76 43 1 152 227 281 353 6.33e-13 69.3
Msa0632340 AT4G18020.10 39.474 76 43 1 152 227 281 353 6.33e-13 69.3
Msa0632340 AT4G18020.7 39.474 76 43 1 152 227 281 353 6.33e-13 69.3
Msa0632340 AT4G18020.3 40.000 75 42 1 152 226 281 352 6.45e-13 69.7
Msa0632340 AT4G18020.2 40.000 75 42 1 152 226 281 352 6.45e-13 69.7
Msa0632340 AT4G18020.1 40.000 75 42 1 152 226 281 352 6.45e-13 69.7
Msa0632340 AT4G18020.6 40.000 75 42 1 152 226 281 352 6.45e-13 69.7
Msa0632340 AT4G18020.9 40.000 75 42 1 152 226 281 352 6.45e-13 69.7
Msa0632340 AT3G04450.4 55.556 54 23 1 170 222 198 251 6.75e-13 69.3
Msa0632340 AT3G04450.2 55.556 54 23 1 170 222 198 251 6.75e-13 69.3
Msa0632340 AT1G69580.1 51.852 54 25 1 170 222 34 87 7.35e-12 65.9
Msa0632340 AT1G69580.2 51.852 54 25 1 170 222 34 87 7.89e-12 65.5
Msa0632340 AT1G25550.1 47.826 69 34 2 156 222 194 262 1.39e-11 65.1
Msa0632340 AT1G14600.1 56.604 53 22 1 172 223 25 77 2.28e-11 63.5
Msa0632340 AT3G24120.1 50.000 68 30 2 170 233 41 108 2.68e-11 63.5
Msa0632340 AT3G24120.2 50.000 68 30 2 170 233 41 108 2.95e-11 63.5
Msa0632340 AT3G13040.3 47.059 68 33 2 169 233 240 307 3.74e-11 63.9
Msa0632340 AT3G13040.1 47.059 68 33 2 169 233 240 307 3.74e-11 63.9
Msa0632340 AT3G13040.2 47.059 68 33 2 169 233 240 307 3.74e-11 63.9
Msa0632340 AT2G40260.1 43.182 88 46 2 136 222 51 135 5.65e-11 63.5
Msa0632340 AT2G20400.1 57.407 54 22 1 170 222 231 284 7.89e-11 62.8
Msa0632340 AT2G20400.2 57.407 54 22 1 170 222 231 284 8.06e-11 62.8

Find 53 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGTTGCCAGCCATTTAC+AGG 0.283862 4_3:+16255199 Msa0632340:CDS
TGCTAAAACGAACCTGTAAA+TGG 0.306750 4_3:-16255211 None:intergenic
GACATTGTGTGGTCGAATTC+AGG 0.320772 4_3:-16255719 None:intergenic
CTTGAATTTGTTGGAAAGAA+AGG 0.326280 4_3:+16254501 Msa0632340:CDS
AGAAACCGCTGATGCAGTTC+TGG 0.337779 4_3:-16255000 None:intergenic
CACATATTGGACTAATCCTT+TGG 0.344008 4_3:+16254798 Msa0632340:CDS
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4_3:+16254492 Msa0632340:CDS
TGAGTTGTAGTGTAACATTT+TGG 0.374181 4_3:-16254585 None:intergenic
GATAAATCATCATCAACATA+TGG 0.380393 4_3:-16255831 None:intergenic
CTTTCTTTCCAACAAATTCA+AGG 0.396613 4_3:-16254500 None:intergenic
ATCAGAAGCTGTATTGCATT+TGG 0.403201 4_3:-16254476 None:intergenic
AATAAAGAATATGAAATTGT+TGG 0.416766 4_3:+16254883 Msa0632340:CDS
ACGCTAAATTGTGTTTCAAA+AGG 0.431875 4_3:-16255654 None:intergenic
TTGAGACCGGCTGGGAATGA+TGG 0.453544 4_3:-16254240 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.460294 4_3:-16254983 None:intergenic
AATAAATTCTTACTAATGAC+AGG 0.461511 4_3:-16254613 None:intergenic
TGTAATCGATATCATTATCA+AGG 0.463829 4_3:+16254315 Msa0632340:CDS
AAGTCATAAACATCCATGTT+TGG 0.465091 4_3:-16254559 None:intergenic
AATGTGGGGCTTGCACATAT+TGG 0.467947 4_3:+16254785 Msa0632340:CDS
TCAACATATGGGAACAAGTT+GGG 0.468173 4_3:-16255819 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_3:+16254974 Msa0632340:CDS
TACTCACTCAATGCCGGAGC+AGG 0.473606 4_3:+16255683 Msa0632340:CDS
AAAAGAAACACATGCAAAGA+AGG 0.478150 4_3:+16254960 Msa0632340:CDS
GCAATAACCTTGAGACCGGC+TGG 0.479637 4_3:-16254249 None:intergenic
GTTCTGGCGACCAAGACAAA+CGG 0.493157 4_3:-16254984 None:intergenic
CTATATGCTGTCAATCAACT+AGG 0.499504 4_3:+16255021 Msa0632340:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4_3:-16255044 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4_3:+16254769 Msa0632340:CDS
ATATGAAATTGTTGGAAGCT+CGG 0.515274 4_3:+16254891 Msa0632340:CDS
TTATGAAGTCTATTGAATGT+GGG 0.532174 4_3:+16254770 Msa0632340:CDS
AAAACGAACCTGTAAATGGC+TGG 0.535893 4_3:-16255207 None:intergenic
CGACACACGACAACAATGGA+TGG 0.540785 4_3:+16255752 Msa0632340:CDS
CACTGTCCTCAACTCTATTG+AGG 0.546156 4_3:+16254287 Msa0632340:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4_3:-16255830 None:intergenic
AGAAAACTCTGGTCCTGCTC+CGG 0.548712 4_3:-16255696 None:intergenic
ATCATTCCCAGCCGGTCTCA+AGG 0.552068 4_3:+16254242 Msa0632340:CDS
ATCAACATATGGGAACAAGT+TGG 0.563419 4_3:-16255820 None:intergenic
AGAAGCTACTCACTCAATGC+CGG 0.575449 4_3:+16255677 Msa0632340:CDS
TGAGACCGGCTGGGAATGAT+GGG 0.577892 4_3:-16254239 None:intergenic
CTTCATAATAGAACTTGCTG+TGG 0.579779 4_3:-16254755 None:intergenic
AAGTGCAATAACCTTGAGAC+CGG 0.582287 4_3:-16254253 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.598969 4_3:+16254771 Msa0632340:CDS
TGATGCTCATATGCCAAACA+TGG 0.604750 4_3:+16254546 Msa0632340:CDS
GGTCGCCAGAACTGCATCAG+CGG 0.609761 4_3:+16254995 Msa0632340:CDS
ATTGCGACACACGACAACAA+TGG 0.611832 4_3:+16255748 Msa0632340:CDS
TCTTGCAACATGTTGCCACA+TGG 0.618977 4_3:-16254839 None:intergenic
GACAACCCATCATTCCCAGC+CGG 0.620153 4_3:+16254234 Msa0632340:CDS
CAATAACCTTGAGACCGGCT+GGG 0.623875 4_3:-16254248 None:intergenic
TCGAATTCAGGAGAAAACTC+TGG 0.627173 4_3:-16255707 None:intergenic
ACATCTCCTCAATAGAGTTG+AGG 0.637933 4_3:-16254293 None:intergenic
GCAATTGTCATGACATTGTG+TGG 0.655655 4_3:-16255730 None:intergenic
TGAAATTGTTGGAAGCTCGG+TGG 0.670504 4_3:+16254894 Msa0632340:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4_3:+16255844 Msa0632340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4_3:16254883-16254902 Msa0632340:CDS 15.0%
!! CATATAGTAAAAAAGTAATT+AGG - chr4_3:16255067-16255086 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4_3:16255517-16255536 Msa0632340:intron 15.0%
!! AATAAAGAATATGAAATTGT+TGG + chr4_3:16254883-16254902 Msa0632340:CDS 15.0%
!! CATATAGTAAAAAAGTAATT+AGG - chr4_3:16255067-16255086 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4_3:16255517-16255536 Msa0632340:intron 15.0%
!!! AGAAACTTTAGAATTGAATA+AGG - chr4_3:16254362-16254381 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_3:16254616-16254635 None:intergenic 20.0%
!! TGGTTCAAAAAAATAGAAAA+CGG + chr4_3:16254914-16254933 Msa0632340:CDS 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4_3:16255122-16255141 Msa0632340:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4_3:16255123-16255142 Msa0632340:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4_3:16255155-16255174 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4_3:16255154-16255173 Msa0632340:CDS 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG - chr4_3:16255186-16255205 None:intergenic 20.0%
!! AAAAATGTTGTTAGTAATGT+CGG - chr4_3:16255790-16255809 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4_3:16255833-16255852 None:intergenic 20.0%
!!! AGAAACTTTAGAATTGAATA+AGG - chr4_3:16254362-16254381 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_3:16254616-16254635 None:intergenic 20.0%
!! TGGTTCAAAAAAATAGAAAA+CGG + chr4_3:16254914-16254933 Msa0632340:CDS 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4_3:16255122-16255141 Msa0632340:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4_3:16255123-16255142 Msa0632340:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4_3:16255155-16255174 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4_3:16255154-16255173 Msa0632340:CDS 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG - chr4_3:16255186-16255205 None:intergenic 20.0%
!! AAAAATGTTGTTAGTAATGT+CGG - chr4_3:16255790-16255809 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4_3:16255833-16255852 None:intergenic 20.0%
! TGTAATCGATATCATTATCA+AGG + chr4_3:16254315-16254334 Msa0632340:CDS 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_3:16254723-16254742 Msa0632340:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_3:16254769-16254788 Msa0632340:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_3:16254770-16254789 Msa0632340:CDS 25.0%
! GGTTCAAAAAAATAGAAAAC+GGG + chr4_3:16254915-16254934 Msa0632340:CDS 25.0%
! GTTCAAAAAAATAGAAAACG+GGG + chr4_3:16254916-16254935 Msa0632340:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4_3:16255124-16255143 Msa0632340:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG - chr4_3:16255185-16255204 None:intergenic 25.0%
! AAAATTACAACAACAGAGTT+AGG - chr4_3:16255311-16255330 None:intergenic 25.0%
! TTTAATAACATGCAAGACTA+AGG - chr4_3:16255357-16255376 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG - chr4_3:16255481-16255500 None:intergenic 25.0%
! GTAGAATGAAAAAATCAAAG+AGG + chr4_3:16255628-16255647 Msa0632340:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4_3:16255834-16255853 None:intergenic 25.0%
! TGTAATCGATATCATTATCA+AGG + chr4_3:16254315-16254334 Msa0632340:CDS 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_3:16254723-16254742 Msa0632340:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_3:16254769-16254788 Msa0632340:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_3:16254770-16254789 Msa0632340:CDS 25.0%
! GGTTCAAAAAAATAGAAAAC+GGG + chr4_3:16254915-16254934 Msa0632340:CDS 25.0%
! GTTCAAAAAAATAGAAAACG+GGG + chr4_3:16254916-16254935 Msa0632340:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4_3:16255124-16255143 Msa0632340:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG - chr4_3:16255185-16255204 None:intergenic 25.0%
! AAAATTACAACAACAGAGTT+AGG - chr4_3:16255311-16255330 None:intergenic 25.0%
! TTTAATAACATGCAAGACTA+AGG - chr4_3:16255357-16255376 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG - chr4_3:16255481-16255500 None:intergenic 25.0%
! GTAGAATGAAAAAATCAAAG+AGG + chr4_3:16255628-16255647 Msa0632340:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4_3:16255834-16255853 None:intergenic 25.0%
CTTTCTTTCCAACAAATTCA+AGG - chr4_3:16254503-16254522 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+AGG + chr4_3:16254501-16254520 Msa0632340:CDS 30.0%
AAGTCATAAACATCCATGTT+TGG - chr4_3:16254562-16254581 None:intergenic 30.0%
! TGAGTTGTAGTGTAACATTT+TGG - chr4_3:16254588-16254607 None:intergenic 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_3:16254771-16254790 Msa0632340:CDS 30.0%
TAATCCTTTGGTTGAAAAAG+AGG + chr4_3:16254810-16254829 Msa0632340:CDS 30.0%
ATATGAAATTGTTGGAAGCT+CGG + chr4_3:16254891-16254910 Msa0632340:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_3:16254960-16254979 Msa0632340:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_3:16255047-16255066 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+TGG - chr4_3:16255463-16255482 None:intergenic 30.0%
!!! CATTCTACCTTTTTTCTCTT+CGG - chr4_3:16255616-16255635 None:intergenic 30.0%
ACGCTAAATTGTGTTTCAAA+AGG - chr4_3:16255657-16255676 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4_3:16255844-16255863 Msa0632340:CDS 30.0%
CTTTCTTTCCAACAAATTCA+AGG - chr4_3:16254503-16254522 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+AGG + chr4_3:16254501-16254520 Msa0632340:CDS 30.0%
AAGTCATAAACATCCATGTT+TGG - chr4_3:16254562-16254581 None:intergenic 30.0%
! TGAGTTGTAGTGTAACATTT+TGG - chr4_3:16254588-16254607 None:intergenic 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_3:16254771-16254790 Msa0632340:CDS 30.0%
TAATCCTTTGGTTGAAAAAG+AGG + chr4_3:16254810-16254829 Msa0632340:CDS 30.0%
ATATGAAATTGTTGGAAGCT+CGG + chr4_3:16254891-16254910 Msa0632340:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_3:16254960-16254979 Msa0632340:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_3:16255047-16255066 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+TGG - chr4_3:16255463-16255482 None:intergenic 30.0%
!!! CATTCTACCTTTTTTCTCTT+CGG - chr4_3:16255616-16255635 None:intergenic 30.0%
ACGCTAAATTGTGTTTCAAA+AGG - chr4_3:16255657-16255676 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4_3:16255844-16255863 Msa0632340:CDS 30.0%
ATCAGAAGCTGTATTGCATT+TGG - chr4_3:16254479-16254498 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_3:16254492-16254511 Msa0632340:CDS 35.0%
!! CTTCATAATAGAACTTGCTG+TGG - chr4_3:16254758-16254777 None:intergenic 35.0%
CACATATTGGACTAATCCTT+TGG + chr4_3:16254798-16254817 Msa0632340:CDS 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4_3:16254817-16254836 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_3:16254959-16254978 None:intergenic 35.0%
CTATATGCTGTCAATCAACT+AGG + chr4_3:16255021-16255040 Msa0632340:CDS 35.0%
TGCTAAAACGAACCTGTAAA+TGG - chr4_3:16255214-16255233 None:intergenic 35.0%
AAAAGATCACTGACAATCCT+AGG - chr4_3:16255287-16255306 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4_3:16255393-16255412 Msa0632340:intron 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4_3:16255822-16255841 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4_3:16255823-16255842 None:intergenic 35.0%
ATCAGAAGCTGTATTGCATT+TGG - chr4_3:16254479-16254498 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_3:16254492-16254511 Msa0632340:CDS 35.0%
!! CTTCATAATAGAACTTGCTG+TGG - chr4_3:16254758-16254777 None:intergenic 35.0%
CACATATTGGACTAATCCTT+TGG + chr4_3:16254798-16254817 Msa0632340:CDS 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4_3:16254817-16254836 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_3:16254959-16254978 None:intergenic 35.0%
CTATATGCTGTCAATCAACT+AGG + chr4_3:16255021-16255040 Msa0632340:CDS 35.0%
TGCTAAAACGAACCTGTAAA+TGG - chr4_3:16255214-16255233 None:intergenic 35.0%
AAAAGATCACTGACAATCCT+AGG - chr4_3:16255287-16255306 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4_3:16255393-16255412 Msa0632340:intron 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4_3:16255822-16255841 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4_3:16255823-16255842 None:intergenic 35.0%
AAGTGCAATAACCTTGAGAC+CGG - chr4_3:16254256-16254275 None:intergenic 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_3:16254296-16254315 None:intergenic 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4_3:16254546-16254565 Msa0632340:CDS 40.0%
AAAAAGAGGTCAAGACCATG+TGG + chr4_3:16254824-16254843 Msa0632340:CDS 40.0%
AAAACGAACCTGTAAATGGC+TGG - chr4_3:16255210-16255229 None:intergenic 40.0%
AATCCTAGGCAACTAGACTT+AGG - chr4_3:16255273-16255292 None:intergenic 40.0%
AGCTCAACCGAAGAGAAAAA+AGG + chr4_3:16255606-16255625 Msa0632340:CDS 40.0%
TCGAATTCAGGAGAAAACTC+TGG - chr4_3:16255710-16255729 None:intergenic 40.0%
GCAATTGTCATGACATTGTG+TGG - chr4_3:16255733-16255752 None:intergenic 40.0%
AAGTGCAATAACCTTGAGAC+CGG - chr4_3:16254256-16254275 None:intergenic 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_3:16254296-16254315 None:intergenic 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4_3:16254546-16254565 Msa0632340:CDS 40.0%
AAAAAGAGGTCAAGACCATG+TGG + chr4_3:16254824-16254843 Msa0632340:CDS 40.0%
AAAACGAACCTGTAAATGGC+TGG - chr4_3:16255210-16255229 None:intergenic 40.0%
AATCCTAGGCAACTAGACTT+AGG - chr4_3:16255273-16255292 None:intergenic 40.0%
AGCTCAACCGAAGAGAAAAA+AGG + chr4_3:16255606-16255625 Msa0632340:CDS 40.0%
TCGAATTCAGGAGAAAACTC+TGG - chr4_3:16255710-16255729 None:intergenic 40.0%
GCAATTGTCATGACATTGTG+TGG - chr4_3:16255733-16255752 None:intergenic 40.0%
CACTGTCCTCAACTCTATTG+AGG + chr4_3:16254287-16254306 Msa0632340:CDS 45.0%
AATGTGGGGCTTGCACATAT+TGG + chr4_3:16254785-16254804 Msa0632340:CDS 45.0%
TCTTGCAACATGTTGCCACA+TGG - chr4_3:16254842-16254861 None:intergenic 45.0%
TGAAATTGTTGGAAGCTCGG+TGG + chr4_3:16254894-16254913 Msa0632340:CDS 45.0%
ACATGTTGCCAGCCATTTAC+AGG + chr4_3:16255199-16255218 Msa0632340:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG + chr4_3:16255267-16255286 Msa0632340:intron 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4_3:16255677-16255696 Msa0632340:CDS 45.0%
GACATTGTGTGGTCGAATTC+AGG - chr4_3:16255722-16255741 None:intergenic 45.0%
ATTGCGACACACGACAACAA+TGG + chr4_3:16255748-16255767 Msa0632340:CDS 45.0%
CACTGTCCTCAACTCTATTG+AGG + chr4_3:16254287-16254306 Msa0632340:CDS 45.0%
AATGTGGGGCTTGCACATAT+TGG + chr4_3:16254785-16254804 Msa0632340:CDS 45.0%
TCTTGCAACATGTTGCCACA+TGG - chr4_3:16254842-16254861 None:intergenic 45.0%
TGAAATTGTTGGAAGCTCGG+TGG + chr4_3:16254894-16254913 Msa0632340:CDS 45.0%
ACATGTTGCCAGCCATTTAC+AGG + chr4_3:16255199-16255218 Msa0632340:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG + chr4_3:16255267-16255286 Msa0632340:intron 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4_3:16255677-16255696 Msa0632340:CDS 45.0%
GACATTGTGTGGTCGAATTC+AGG - chr4_3:16255722-16255741 None:intergenic 45.0%
ATTGCGACACACGACAACAA+TGG + chr4_3:16255748-16255767 Msa0632340:CDS 45.0%
CAATAACCTTGAGACCGGCT+GGG - chr4_3:16254251-16254270 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_3:16254974-16254993 Msa0632340:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_3:16254986-16255005 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_3:16254987-16255006 None:intergenic 50.0%
AGAAACCGCTGATGCAGTTC+TGG - chr4_3:16255003-16255022 None:intergenic 50.0%
AGAAAACTCTGGTCCTGCTC+CGG - chr4_3:16255699-16255718 None:intergenic 50.0%
CGACACACGACAACAATGGA+TGG + chr4_3:16255752-16255771 Msa0632340:CDS 50.0%
CAATAACCTTGAGACCGGCT+GGG - chr4_3:16254251-16254270 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_3:16254974-16254993 Msa0632340:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_3:16254986-16255005 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_3:16254987-16255006 None:intergenic 50.0%
AGAAACCGCTGATGCAGTTC+TGG - chr4_3:16255003-16255022 None:intergenic 50.0%
AGAAAACTCTGGTCCTGCTC+CGG - chr4_3:16255699-16255718 None:intergenic 50.0%
CGACACACGACAACAATGGA+TGG + chr4_3:16255752-16255771 Msa0632340:CDS 50.0%
GACAACCCATCATTCCCAGC+CGG + chr4_3:16254234-16254253 Msa0632340:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4_3:16254242-16254261 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4_3:16254243-16254262 None:intergenic 55.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4_3:16254242-16254261 Msa0632340:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4_3:16254252-16254271 None:intergenic 55.0%
TACTCACTCAATGCCGGAGC+AGG + chr4_3:16255683-16255702 Msa0632340:CDS 55.0%
GACAACCCATCATTCCCAGC+CGG + chr4_3:16254234-16254253 Msa0632340:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4_3:16254242-16254261 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4_3:16254243-16254262 None:intergenic 55.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4_3:16254242-16254261 Msa0632340:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4_3:16254252-16254271 None:intergenic 55.0%
TACTCACTCAATGCCGGAGC+AGG + chr4_3:16255683-16255702 Msa0632340:CDS 55.0%
GGTCGCCAGAACTGCATCAG+CGG + chr4_3:16254995-16255014 Msa0632340:CDS 60.0%
GGTCGCCAGAACTGCATCAG+CGG + chr4_3:16254995-16255014 Msa0632340:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 16254219 16255869 16254219 ID=Msa0632340;Name=Msa0632340
chr4_3 mRNA 16254219 16255869 16254219 ID=Msa0632340-mRNA-1;Parent=Msa0632340;Name=Msa0632340-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|5|0|313
chr4_3 exon 16254219 16254336 16254219 ID=Msa0632340-mRNA-1:exon:4596;Parent=Msa0632340-mRNA-1
chr4_3 exon 16254473 16254622 16254473 ID=Msa0632340-mRNA-1:exon:4597;Parent=Msa0632340-mRNA-1
chr4_3 exon 16254740 16255051 16254740 ID=Msa0632340-mRNA-1:exon:4598;Parent=Msa0632340-mRNA-1
chr4_3 exon 16255144 16255220 16255144 ID=Msa0632340-mRNA-1:exon:4599;Parent=Msa0632340-mRNA-1
chr4_3 exon 16255585 16255869 16255585 ID=Msa0632340-mRNA-1:exon:4600;Parent=Msa0632340-mRNA-1
chr4_3 CDS 16254219 16254336 16254219 ID=Msa0632340-mRNA-1:cds;Parent=Msa0632340-mRNA-1
chr4_3 CDS 16254473 16254622 16254473 ID=Msa0632340-mRNA-1:cds;Parent=Msa0632340-mRNA-1
chr4_3 CDS 16254740 16255051 16254740 ID=Msa0632340-mRNA-1:cds;Parent=Msa0632340-mRNA-1
chr4_3 CDS 16255144 16255220 16255144 ID=Msa0632340-mRNA-1:cds;Parent=Msa0632340-mRNA-1
chr4_3 CDS 16255585 16255869 16255585 ID=Msa0632340-mRNA-1:cds;Parent=Msa0632340-mRNA-1
Gene Sequence

>Msa0632340

ATGGCAGTTTTCACTGACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCACTTGATCATGACATCACTGTCCTCAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGTTACCAAATGCAATACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAAACATGGATGTTTATGACTTTGTCCAAAATGTTACACTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAGCAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCACATATTGGACTAATCCTTTGGTTGAAAAAGAGGTCAAGACCATGTGGCAACATGTTGCAAGAATTAGAAACTGTTTGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTCGGTGGTTCAAAAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAACTGCATCAGCGGTTTCTATATGCTGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGAAGTATAGAAATTATTTAAAGAGCTCAACCGAAGAGAAAAAAGGTAGAATGAAAAAATCAAAGAGGAGTCCTTTTGAAACACAATTTAGCGTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCGACCACACAATGTCATGACAATTGCGACACACGACAACAATGGATGGAAATTAGTGAATCCGACATTACTAACAACATTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>Msa0632340

MAVFTDNPSFPAGLKVIALDHDITVLNSIEEMCNRYHYQVTKCNTASDALNLLERKDCFDVMLIDAHMPNMDVYDFVQNVTLQLNIPVIMMAVDSTASSIMKSIECGACTYWTNPLVEKEVKTMWQHVARIRNCLTENKEYEIVGSSVVQKNRKRGREDANASKETHAKKARLSWSPELHQRFLYAVNQLGLDRATPKKILKIMDVPDMKKEHVASHLQKYRNYLKSSTEEKKGRMKKSKRSPFETQFSVEATHSMPEQDQSFLLNSTTQCHDNCDTRQQWMEISESDITNNIFSDLPNLFPYVDDDLSSLVW*