AlfalfaGEDB | Msa0632800

M. sativa cultivar zhongmu-4 / Msa0632800

KEGG: -
GO: -
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632800	XP_013455733.1	54.980	251	92	5	1	248	1	233	4.24e-77	243

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632800	sp\|Q4PSU4\|AGL61_ARATH	35.417	192	106	5	7	187	61	245	3.70e-21	92.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632800	A0A072UJ33	54.980	251	92	5	1	248	1	233	2.03e-77	243

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TFs/TRs:

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Gene ID	Type	Classification
Msa0632800	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632800	MtrunA17_Chr4g0025531	54.980	251	92	5	1	248	1	233	3.90e-81	243
Msa0632800	MtrunA17_Chr3g0135891	59.259	135	49	4	106	238	16	146	1.58e-41	139
Msa0632800	MtrunA17_Chr1g0206701	34.375	224	115	8	1	192	1	224	5.58e-24	96.3
Msa0632800	MtrunA17_Chr3g0099421	37.725	167	87	5	1	150	26	192	1.51e-23	95.9
Msa0632800	MtrunA17_Chr1g0149391	38.506	174	85	8	1	156	1	170	2.53e-23	94.7
Msa0632800	MtrunA17_Chr2g0292791	38.323	167	86	5	1	150	1	167	8.82e-23	93.2
Msa0632800	MtrunA17_Chr1g0206991	37.888	161	83	5	1	144	1	161	1.07e-22	91.7
Msa0632800	MtrunA17_Chr8g0355091	38.411	151	78	4	1	136	1	151	1.12e-22	92.8
Msa0632800	MtrunA17_Chr1g0208561	33.735	166	97	3	1	156	1	163	2.22e-22	92.0
Msa0632800	MtrunA17_Chr3g0119901	34.940	166	93	4	1	155	1	162	4.58e-22	90.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632800	AT4G36590.1	35.928	167	90	3	5	156	3	167	7.57e-24	96.7
Msa0632800	AT2G24840.1	35.417	192	106	5	7	187	61	245	3.76e-22	92.4
Msa0632800	AT1G29962.1	34.659	176	97	4	1	161	1	173	8.63e-22	89.7
Msa0632800	AT1G28460.1	34.682	173	98	4	1	161	1	170	5.98e-20	84.7
Msa0632800	AT1G01530.1	38.136	118	67	2	5	117	3	119	2.76e-18	81.6
Msa0632800	AT5G60440.1	31.925	213	115	6	5	205	3	197	2.98e-18	82.4
Msa0632800	AT1G28450.1	32.184	174	100	4	1	159	1	171	7.47e-17	76.6
Msa0632800	AT2G34440.1	32.298	161	94	5	9	159	2	157	2.51e-16	74.7
Msa0632800	AT1G65360.1	33.962	106	66	1	5	106	3	108	5.35e-16	75.1
Msa0632800	AT3G04100.1	41.573	89	49	1	2	90	7	92	8.55e-16	73.9

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Find 59 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGACACATCTTTAG+TGG	0.370003	4_3:+17241921	Msa0632800:CDS
AACAACAACGGTTATGAAAT+TGG	0.254239	4_3:+17242187	Msa0632800:CDS
AATTTCATAACCGTTGTTGT+TGG	0.462889	4_3:-17242185	None:intergenic
ACAAAGTGTTGATAATTCAG+AGG	0.616841	4_3:-17241611	None:intergenic
ACCAATCCTCCTTATGTCTT+TGG	0.213662	4_3:+17242160	Msa0632800:CDS
ACGGGAAAGTTTGAAGGCTT+TGG	0.328236	4_3:+17241834	Msa0632800:CDS
AGACATAAGGAGGATTGGTC+TGG	0.500564	4_3:-17242156	None:intergenic
AGCACAACGGGAAAGTTTGA+AGG	0.506480	4_3:+17241828	Msa0632800:CDS
AGCATGTTGCGGAAGCACAA+CGG	0.628936	4_3:+17241815	Msa0632800:CDS
AGTAGTGTCTTAAGCAATGG+AGG	0.611596	4_3:+17241964	Msa0632800:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTGAATTATCAACACTTTG+TGG	+	chr4_3:17241613-17241632	Msa0632800:CDS	30.0%
CTGAATTATCAACACTTTGT+GGG	+	chr4_3:17241614-17241633	Msa0632800:CDS	30.0%
TGAATTATCAACACTTTGTG+GGG	+	chr4_3:17241615-17241634	Msa0632800:CDS	30.0%
CTTCAAAATTGTTCTTGCTA+TGG	-	chr4_3:17241710-17241729	None:intergenic	30.0%
AAAATTGGACACATCTTTAG+TGG	+	chr4_3:17241921-17241940	Msa0632800:CDS	30.0%
ATAAGTAGTGTCTTAAGCAA+TGG	+	chr4_3:17241961-17241980	Msa0632800:CDS	30.0%
TGTACATCTTCTAATCAACT+TGG	+	chr4_3:17242009-17242028	Msa0632800:intron	30.0%
AATTTCATAACCGTTGTTGT+TGG	-	chr4_3:17242188-17242207	None:intergenic	30.0%
AACAACAACGGTTATGAAAT+TGG	+	chr4_3:17242187-17242206	Msa0632800:CDS	30.0%
GAAGGTAGAAATAAAACACA+AGG	+	chr4_3:17242500-17242519	Msa0632800:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	17241496	17242842	17241496	ID=Msa0632800;Name=Msa0632800
chr4_3	mRNA	17241496	17242842	17241496	ID=Msa0632800-mRNA-1;Parent=Msa0632800;Name=Msa0632800-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|250\|249
chr4_3	exon	17241496	17242003	17241496	ID=Msa0632800-mRNA-1:exon:4821;Parent=Msa0632800-mRNA-1
chr4_3	exon	17242125	17242214	17242125	ID=Msa0632800-mRNA-1:exon:4822;Parent=Msa0632800-mRNA-1
chr4_3	exon	17242441	17242842	17242441	ID=Msa0632800-mRNA-1:exon:4823;Parent=Msa0632800-mRNA-1
chr4_3	CDS	17241496	17242003	17241496	ID=Msa0632800-mRNA-1:cds;Parent=Msa0632800-mRNA-1
chr4_3	CDS	17242125	17242214	17242125	ID=Msa0632800-mRNA-1:cds;Parent=Msa0632800-mRNA-1
chr4_3	CDS	17242441	17242592	17242441	ID=Msa0632800-mRNA-1:cds;Parent=Msa0632800-mRNA-1
chr4_3	three_prime_UTR	17242593	17242842	17242593	ID=Msa0632800-mRNA-1:three_prime_utr;Parent=Msa0632800-mRNA-1

Gene Sequence

>Msa0632800

ATGGCTCCGAAGAGGACATCAAGAGGTAGACAAAAAAGAGAAATGAAGAAGATTGAGAGAGAAAGCGATCTTTTAGTAGCATTTTCAAAGAGAAAGTCTGGCATTTACAACAAAGCCTCTGAATTATCAACACTTTGTGGGGCTTTAGTTGATATTCTTATTATTTCTCCAGCAGGAAAACCATATTGTTATGGAGAACCTTCTTCTCAATCCATAGCAAGAACAATTTTGAAGAATGAAAACCCTTCAGTAGAAGAAGAATTAAAGCAACTAAATATTAACCAAGTTAACACAAAAAATGATGAGCTTTTGGATGAAAAGCATGTTGCGGAAGCACAACGGGAAAGTTTGAAGGCTTTGGAAAGTTATGGCTATTGGTCGATCAATGAGAAAGATGTTGAAAGCTTTGATTATGACCAGTTAAAAAAATTGGACACATCTTTAGTGGACATTGCAAATAAATTGATAAGTAGTGTCTTAAGCAATGGAGGAGGGATTAATTCTCATGCAATTTCTTGCATAGACGAACTGAATGGAGTCCAGACCAATCCTCCTTATGTCTTTGGGGCCAACAACAACGGTTATGAAATTGGTGCAACTCATGGATCCCAGATTTATGATCCAAAAGGAAAAGGCAAGGATGTTATTGCTTCTCTTGAAGGTAGAAATAAAACACAAGGACCAAACCTTGACCGTGCAAGTGAAAGTGGAAATGGAAGTGATGTTGATCATTCTCCTAAAGATGAATGA

Protein sequence

>Msa0632800

MAPKRTSRGRQKREMKKIERESDLLVAFSKRKSGIYNKASELSTLCGALVDILIISPAGKPYCYGEPSSQSIARTILKNENPSVEEELKQLNINQVNTKNDELLDEKHVAEAQRESLKALESYGYWSINEKDVESFDYDQLKKLDTSLVDIANKLISSVLSNGGGINSHAISCIDELNGVQTNPPYVFGANNNGYEIGATHGSQIYDPKGKGKDVIASLEGRNKTQGPNLDRASESGNGSDVDHSPKDE*