AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0632800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0632800 MtrunA17_Chr4g0025531 54.980 251 92 5 1 248 1 233 3.90e-81 243
Msa0632800 MtrunA17_Chr3g0135891 59.259 135 49 4 106 238 16 146 1.58e-41 139
Msa0632800 MtrunA17_Chr1g0206701 34.375 224 115 8 1 192 1 224 5.58e-24 96.3
Msa0632800 MtrunA17_Chr3g0099421 37.725 167 87 5 1 150 26 192 1.51e-23 95.9
Msa0632800 MtrunA17_Chr1g0149391 38.506 174 85 8 1 156 1 170 2.53e-23 94.7
Msa0632800 MtrunA17_Chr2g0292791 38.323 167 86 5 1 150 1 167 8.82e-23 93.2
Msa0632800 MtrunA17_Chr1g0206991 37.888 161 83 5 1 144 1 161 1.07e-22 91.7
Msa0632800 MtrunA17_Chr8g0355091 38.411 151 78 4 1 136 1 151 1.12e-22 92.8
Msa0632800 MtrunA17_Chr1g0208561 33.735 166 97 3 1 156 1 163 2.22e-22 92.0
Msa0632800 MtrunA17_Chr3g0119901 34.940 166 93 4 1 155 1 162 4.58e-22 90.5
Msa0632800 MtrunA17_Chr3g0089141 33.735 166 97 3 1 156 1 163 4.69e-22 90.9
Msa0632800 MtrunA17_Chr5g0422421 31.163 215 114 8 1 201 1 195 2.00e-21 89.7
Msa0632800 MtrunA17_Chr1g0206721 37.748 151 79 4 1 136 1 151 5.23e-21 88.6
Msa0632800 MtrunA17_Chr3g0085241 31.222 221 121 7 4 202 36 247 5.31e-21 88.6
Msa0632800 MtrunA17_Chr1g0188671 30.964 197 112 4 1 187 39 221 9.58e-21 88.6
Msa0632800 MtrunA17_Chr1g0187361 32.203 177 102 5 1 163 1 173 4.47e-20 85.9
Msa0632800 MtrunA17_Chr1g0208581 33.540 161 90 5 1 144 1 161 5.72e-20 85.5
Msa0632800 MtrunA17_Chr3g0099411 42.149 121 60 3 1 111 1 121 1.13e-19 84.3
Msa0632800 MtrunA17_Chr2g0317451 30.000 160 101 3 1 151 1 158 1.81e-19 84.3
Msa0632800 MtrunA17_Chr2g0317481 30.000 160 101 3 1 151 1 158 1.97e-19 84.3
Msa0632800 MtrunA17_Chr8g0379021 32.704 159 98 4 4 156 7 162 3.86e-19 82.0
Msa0632800 MtrunA17_Chr5g0419601 35.507 138 77 3 1 127 1 137 7.45e-19 82.8
Msa0632800 MtrunA17_Chr2g0325971 32.919 161 91 4 1 144 1 161 1.53e-18 81.6
Msa0632800 MtrunA17_Chr2g0333981 28.750 160 103 3 4 156 7 162 4.29e-18 79.3
Msa0632800 MtrunA17_Chr5g0422941 37.864 103 58 1 1 97 1 103 4.84e-18 80.5
Msa0632800 MtrunA17_Chr3g0111841 53.448 58 27 0 15 72 2 59 3.05e-16 71.2
Msa0632800 MtrunA17_Chr2g0300421 44.156 77 43 0 1 77 1 77 4.94e-16 73.6
Msa0632800 MtrunA17_Chr4g0052081 50.725 69 34 0 9 77 2 70 4.94e-16 73.6
Msa0632800 MtrunA17_Chr3g0089151 45.833 72 39 0 1 72 1 72 6.17e-16 72.4
Msa0632800 MtrunA17_Chr8g0356071 34.513 113 61 2 9 115 2 107 3.53e-15 70.9
Msa0632800 MtrunA17_Chr4g0052061 30.263 152 78 3 9 151 2 134 7.82e-15 70.1
Msa0632800 MtrunA17_Chr1g0206691 31.333 150 89 4 18 157 1 146 1.42e-14 70.5
Msa0632800 MtrunA17_Chr2g0300441 30.120 166 99 4 1 149 1 166 1.68e-14 70.9
Msa0632800 MtrunA17_Chr8g0345001 32.515 163 94 4 8 159 17 174 2.66e-14 69.3
Msa0632800 MtrunA17_Chr8g0376821 41.250 80 46 1 9 87 2 81 5.67e-14 68.2
Msa0632800 MtrunA17_Chr1g0169761 31.447 159 81 5 9 141 2 158 1.32e-13 67.0
Msa0632800 MtrunA17_Chr8g0357771 40.278 72 43 0 9 80 2 73 2.46e-13 66.6
Msa0632800 MtrunA17_Chr4g0074101 34.314 102 58 2 9 101 2 103 5.61e-13 65.9
Msa0632800 MtrunA17_Chr1g0175611 40.000 70 42 0 9 78 2 71 1.91e-12 63.5
Msa0632800 MtrunA17_Chr1g0159661 50.000 64 32 0 9 72 2 65 5.76e-12 62.8
Msa0632800 MtrunA17_Chr1g0180591 34.694 98 63 1 9 105 2 99 6.84e-12 62.4
Msa0632800 MtrunA17_Chr4g0018951 29.921 127 83 4 26 148 29 153 1.29e-11 61.6
Msa0632800 MtrunA17_Chr1g0187351 38.462 65 40 0 18 82 1 65 2.36e-11 58.2
Msa0632800 MtrunA17_Chr4g0057151 49.254 67 31 1 10 76 3 66 2.69e-11 61.2
Msa0632800 MtrunA17_Chr1g0175601 40.000 65 39 0 9 73 2 66 3.96e-11 60.5
Msa0632800 MtrunA17_Chr4g0071601 26.347 167 108 4 4 156 7 172 5.01e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0632800 AT4G36590.1 35.928 167 90 3 5 156 3 167 7.57e-24 96.7
Msa0632800 AT2G24840.1 35.417 192 106 5 7 187 61 245 3.76e-22 92.4
Msa0632800 AT1G29962.1 34.659 176 97 4 1 161 1 173 8.63e-22 89.7
Msa0632800 AT1G28460.1 34.682 173 98 4 1 161 1 170 5.98e-20 84.7
Msa0632800 AT1G01530.1 38.136 118 67 2 5 117 3 119 2.76e-18 81.6
Msa0632800 AT5G60440.1 31.925 213 115 6 5 205 3 197 2.98e-18 82.4
Msa0632800 AT1G28450.1 32.184 174 100 4 1 159 1 171 7.47e-17 76.6
Msa0632800 AT2G34440.1 32.298 161 94 5 9 159 2 157 2.51e-16 74.7
Msa0632800 AT1G65360.1 33.962 106 66 1 5 106 3 108 5.35e-16 75.1
Msa0632800 AT3G04100.1 41.573 89 49 1 2 90 7 92 8.55e-16 73.9
Msa0632800 AT3G66656.1 47.692 65 34 0 9 73 2 66 5.62e-14 68.6
Msa0632800 AT1G47760.1 39.759 83 47 1 9 91 2 81 6.98e-13 65.5
Msa0632800 AT4G11880.2 49.254 67 32 1 10 76 3 67 5.48e-11 60.5
Msa0632800 AT4G11880.3 49.254 67 32 1 10 76 3 67 5.48e-11 60.5
Msa0632800 AT4G11880.4 49.254 67 32 1 10 76 3 67 8.27e-11 60.5
Msa0632800 AT4G11880.1 49.254 67 32 1 10 76 3 67 8.27e-11 60.5
Msa0632800 AT5G10140.2 48.214 56 29 0 9 64 2 57 9.44e-11 59.3

Find 59 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCATATCATTCATCTTT+AGG 0.191781 4_3:-17242578 None:intergenic
ACCAATCCTCCTTATGTCTT+TGG 0.213662 4_3:+17242160 Msa0632800:CDS
CCAATCCTCCTTATGTCTTT+GGG 0.233763 4_3:+17242161 Msa0632800:CDS
AACAACAACGGTTATGAAAT+TGG 0.254239 4_3:+17242187 Msa0632800:CDS
ATCATATATACCTTGGAATA+AGG 0.308845 4_3:+17242621 Msa0632800:three_prime_UTR
ACGGGAAAGTTTGAAGGCTT+TGG 0.328236 4_3:+17241834 Msa0632800:CDS
ATAAGCGGACATCACATTTC+AGG 0.330823 4_3:+17242648 Msa0632800:three_prime_UTR
GAAGGTTCTCCATAACAATA+TGG 0.353627 4_3:-17241676 None:intergenic
CTTTGGAAAGTTATGGCTAT+TGG 0.358280 4_3:+17241851 Msa0632800:CDS
AAAATTGGACACATCTTTAG+TGG 0.370003 4_3:+17241921 Msa0632800:CDS
GATGTTATTGCTTCTCTTGA+AGG 0.392101 4_3:+17242482 Msa0632800:CDS
TCTGAATTATCAACACTTTG+TGG 0.392582 4_3:+17241613 Msa0632800:CDS
TTGAAGGCTTTGGAAAGTTA+TGG 0.393387 4_3:+17241844 Msa0632800:CDS
CTTGCTATGGATTGAGAAGA+AGG 0.408194 4_3:-17241694 None:intergenic
GCATGTTGCGGAAGCACAAC+GGG 0.408411 4_3:+17241816 Msa0632800:CDS
GCAGGAAAACCATATTGTTA+TGG 0.420932 4_3:+17241667 Msa0632800:CDS
TCACTTGCACGGTCAAGGTT+TGG 0.423194 4_3:-17242524 None:intergenic
CTGAATTATCAACACTTTGT+GGG 0.424852 4_3:+17241614 Msa0632800:CDS
CTACCTCTTGATGTCCTCTT+CGG 0.429458 4_3:-17241502 None:intergenic
CTTCAAAATTGTTCTTGCTA+TGG 0.431100 4_3:-17241707 None:intergenic
CATCAAAATGAGAAAATAGT+TGG 0.433292 4_3:-17242683 None:intergenic
GGTATATATGATCATACATA+TGG 0.438624 4_3:-17242610 None:intergenic
TTTAATTCTTCTTCTACTGA+AGG 0.442448 4_3:-17241739 None:intergenic
CATTTCCACTTTCACTTGCA+CGG 0.448306 4_3:-17242535 None:intergenic
ATTCTTATTATTTCTCCAGC+AGG 0.453510 4_3:+17241649 Msa0632800:CDS
TGAGAAAATAGTTGGAATCA+TGG 0.457232 4_3:-17242675 None:intergenic
CACTTTCACTTGCACGGTCA+AGG 0.460125 4_3:-17242529 None:intergenic
AATTTCATAACCGTTGTTGT+TGG 0.462889 4_3:-17242185 None:intergenic
CTTGACCGTGCAAGTGAAAG+TGG 0.475104 4_3:+17242530 Msa0632800:CDS
TTGTTGGCCCCAAAGACATA+AGG 0.478695 4_3:-17242169 None:intergenic
TTAATTCTTCTTCTACTGAA+GGG 0.481788 4_3:-17241738 None:intergenic
CGTGCAAGTGAAAGTGGAAA+TGG 0.489776 4_3:+17242536 Msa0632800:CDS
AGACATAAGGAGGATTGGTC+TGG 0.500564 4_3:-17242156 None:intergenic
AGCACAACGGGAAAGTTTGA+AGG 0.506480 4_3:+17241828 Msa0632800:CDS
ATTTATGATCCAAAAGGAAA+AGG 0.506756 4_3:+17242455 Msa0632800:CDS
CCCAAAGACATAAGGAGGAT+TGG 0.507911 4_3:-17242161 None:intergenic
TCCCAGATTTATGATCCAAA+AGG 0.515467 4_3:+17242449 Msa0632800:CDS
TAAGCGGACATCACATTTCA+GGG 0.527919 4_3:+17242649 Msa0632800:three_prime_UTR
TCTTGCATAGACGAACTGAA+TGG 0.536180 4_3:+17242130 Msa0632800:CDS
ATAAGTAGTGTCTTAAGCAA+TGG 0.548667 4_3:+17241961 Msa0632800:CDS
GCTTATATCACCTTATTCCA+AGG 0.555747 4_3:-17242631 None:intergenic
ATGTATGATCATATATACCT+TGG 0.560368 4_3:+17242614 Msa0632800:three_prime_UTR
TGAATTATCAACACTTTGTG+GGG 0.569118 4_3:+17241615 Msa0632800:CDS
GTCTTTGGGGCCAACAACAA+CGG 0.574083 4_3:+17242175 Msa0632800:CDS
AGTGTCTTAAGCAATGGAGG+AGG 0.588530 4_3:+17241967 Msa0632800:CDS
CAATCCTCCTTATGTCTTTG+GGG 0.592798 4_3:+17242162 Msa0632800:CDS
GTGTCTTAAGCAATGGAGGA+GGG 0.600823 4_3:+17241968 Msa0632800:CDS
GGAGGAGGGATTAATTCTCA+TGG 0.603996 4_3:+17241982 Msa0632800:CDS
AGTAGTGTCTTAAGCAATGG+AGG 0.611596 4_3:+17241964 Msa0632800:CDS
TTGGCCCCAAAGACATAAGG+AGG 0.611952 4_3:-17242166 None:intergenic
ACAAAGTGTTGATAATTCAG+AGG 0.616841 4_3:-17241611 None:intergenic
TGATCCAAAAGGAAAAGGCA+AGG 0.624272 4_3:+17242460 Msa0632800:CDS
AGCATGTTGCGGAAGCACAA+CGG 0.628936 4_3:+17241815 Msa0632800:CDS
TTTGGATGAAAAGCATGTTG+CGG 0.629913 4_3:+17241804 Msa0632800:CDS
TGGTTAAGATGGCTCCGAAG+AGG 0.660173 4_3:+17241488 None:intergenic
TTGGAATAAGGTGATATAAG+CGG 0.660825 4_3:+17242633 Msa0632800:three_prime_UTR
GCTCCGAAGAGGACATCAAG+AGG 0.673948 4_3:+17241499 Msa0632800:CDS
GAAGGTAGAAATAAAACACA+AGG 0.705496 4_3:+17242500 Msa0632800:CDS
GCGGACATCACATTTCAGGG+TGG 0.773228 4_3:+17242652 Msa0632800:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATTTATGAAAAAG+TGG + chr4_3:17242085-17242104 Msa0632800:intron 15.0%
!! CTAATTTATTTATGAAAAAG+TGG + chr4_3:17242085-17242104 Msa0632800:intron 15.0%
!! TTATGACCAGTTAAAAAAAT+TGG + chr4_3:17241906-17241925 Msa0632800:CDS 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG - chr4_3:17242360-17242379 None:intergenic 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG - chr4_3:17242361-17242380 None:intergenic 20.0%
!! GAAATTATACAATATGTGTT+AGG - chr4_3:17242409-17242428 None:intergenic 20.0%
!!! TTTTCTATATTCTACTTTTG+TGG + chr4_3:17242742-17242761 Msa0632800:three_prime_UTR 20.0%
!! TTATGACCAGTTAAAAAAAT+TGG + chr4_3:17241906-17241925 Msa0632800:CDS 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG - chr4_3:17242360-17242379 None:intergenic 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG - chr4_3:17242361-17242380 None:intergenic 20.0%
!! GAAATTATACAATATGTGTT+AGG - chr4_3:17242409-17242428 None:intergenic 20.0%
!!! TTTTCTATATTCTACTTTTG+TGG + chr4_3:17242742-17242761 Msa0632800:three_prime_UTR 20.0%
! TTAATTCTTCTTCTACTGAA+GGG - chr4_3:17241741-17241760 None:intergenic 25.0%
! TTTAATTCTTCTTCTACTGA+AGG - chr4_3:17241742-17241761 None:intergenic 25.0%
!!! CATCATTTTTTGTGTTAACT+TGG - chr4_3:17241780-17241799 None:intergenic 25.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG - chr4_3:17241915-17241934 None:intergenic 25.0%
! ACTCTTGTGTTATATGAATT+TGG - chr4_3:17242233-17242252 None:intergenic 25.0%
! AGTATAGATATTTGATGTGT+TGG - chr4_3:17242275-17242294 None:intergenic 25.0%
! GCATTAAAAATGGATAAAGT+TGG - chr4_3:17242299-17242318 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG - chr4_3:17242309-17242328 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG + chr4_3:17242455-17242474 Msa0632800:CDS 25.0%
! GGTATATATGATCATACATA+TGG - chr4_3:17242613-17242632 None:intergenic 25.0%
! ATGTATGATCATATATACCT+TGG + chr4_3:17242614-17242633 Msa0632800:three_prime_UTR 25.0%
! ATCATATATACCTTGGAATA+AGG + chr4_3:17242621-17242640 Msa0632800:three_prime_UTR 25.0%
! CATCAAAATGAGAAAATAGT+TGG - chr4_3:17242686-17242705 None:intergenic 25.0%
!!! TTTGTGGATTTTTATTTGAG+TGG + chr4_3:17242758-17242777 Msa0632800:three_prime_UTR 25.0%
!!! TCAGTTTTTTTTTTGTGCTT+TGG + chr4_3:17242802-17242821 Msa0632800:three_prime_UTR 25.0%
!!! TTTTTTTGTGCTTTGGATTT+TGG + chr4_3:17242809-17242828 Msa0632800:three_prime_UTR 25.0%
! TTAATTCTTCTTCTACTGAA+GGG - chr4_3:17241741-17241760 None:intergenic 25.0%
! TTTAATTCTTCTTCTACTGA+AGG - chr4_3:17241742-17241761 None:intergenic 25.0%
!!! CATCATTTTTTGTGTTAACT+TGG - chr4_3:17241780-17241799 None:intergenic 25.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG - chr4_3:17241915-17241934 None:intergenic 25.0%
! ACTCTTGTGTTATATGAATT+TGG - chr4_3:17242233-17242252 None:intergenic 25.0%
! AGTATAGATATTTGATGTGT+TGG - chr4_3:17242275-17242294 None:intergenic 25.0%
! GCATTAAAAATGGATAAAGT+TGG - chr4_3:17242299-17242318 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG - chr4_3:17242309-17242328 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG + chr4_3:17242455-17242474 Msa0632800:CDS 25.0%
! GGTATATATGATCATACATA+TGG - chr4_3:17242613-17242632 None:intergenic 25.0%
! ATGTATGATCATATATACCT+TGG + chr4_3:17242614-17242633 Msa0632800:three_prime_UTR 25.0%
! ATCATATATACCTTGGAATA+AGG + chr4_3:17242621-17242640 Msa0632800:three_prime_UTR 25.0%
! CATCAAAATGAGAAAATAGT+TGG - chr4_3:17242686-17242705 None:intergenic 25.0%
!!! TTTGTGGATTTTTATTTGAG+TGG + chr4_3:17242758-17242777 Msa0632800:three_prime_UTR 25.0%
!!! TCAGTTTTTTTTTTGTGCTT+TGG + chr4_3:17242802-17242821 Msa0632800:three_prime_UTR 25.0%
!!! TTTTTTTGTGCTTTGGATTT+TGG + chr4_3:17242809-17242828 Msa0632800:three_prime_UTR 25.0%
!! ACAAAGTGTTGATAATTCAG+AGG - chr4_3:17241614-17241633 None:intergenic 30.0%
TCTGAATTATCAACACTTTG+TGG + chr4_3:17241613-17241632 Msa0632800:CDS 30.0%
CTGAATTATCAACACTTTGT+GGG + chr4_3:17241614-17241633 Msa0632800:CDS 30.0%
TGAATTATCAACACTTTGTG+GGG + chr4_3:17241615-17241634 Msa0632800:CDS 30.0%
! ATTCTTATTATTTCTCCAGC+AGG + chr4_3:17241649-17241668 Msa0632800:CDS 30.0%
CTTCAAAATTGTTCTTGCTA+TGG - chr4_3:17241710-17241729 None:intergenic 30.0%
! CACAAAAAATGATGAGCTTT+TGG + chr4_3:17241786-17241805 Msa0632800:CDS 30.0%
AAAATTGGACACATCTTTAG+TGG + chr4_3:17241921-17241940 Msa0632800:CDS 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG + chr4_3:17241961-17241980 Msa0632800:CDS 30.0%
TGTACATCTTCTAATCAACT+TGG + chr4_3:17242009-17242028 Msa0632800:intron 30.0%
AATTTCATAACCGTTGTTGT+TGG - chr4_3:17242188-17242207 None:intergenic 30.0%
AACAACAACGGTTATGAAAT+TGG + chr4_3:17242187-17242206 Msa0632800:CDS 30.0%
! TCCTTTTGGATCATAAATCT+GGG - chr4_3:17242453-17242472 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATC+TGG - chr4_3:17242454-17242473 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG + chr4_3:17242500-17242519 Msa0632800:CDS 30.0%
CTAGCATATCATTCATCTTT+AGG - chr4_3:17242581-17242600 None:intergenic 30.0%
TTGGAATAAGGTGATATAAG+CGG + chr4_3:17242633-17242652 Msa0632800:three_prime_UTR 30.0%
TGAGAAAATAGTTGGAATCA+TGG - chr4_3:17242678-17242697 None:intergenic 30.0%
!! TGGATTTTTATTTGAGTGGT+TGG + chr4_3:17242762-17242781 Msa0632800:three_prime_UTR 30.0%
!!! CTTTGGATTTTGGTTGTATT+GGG + chr4_3:17242819-17242838 Msa0632800:three_prime_UTR 30.0%
!! ACAAAGTGTTGATAATTCAG+AGG - chr4_3:17241614-17241633 None:intergenic 30.0%
TCTGAATTATCAACACTTTG+TGG + chr4_3:17241613-17241632 Msa0632800:CDS 30.0%
CTGAATTATCAACACTTTGT+GGG + chr4_3:17241614-17241633 Msa0632800:CDS 30.0%
TGAATTATCAACACTTTGTG+GGG + chr4_3:17241615-17241634 Msa0632800:CDS 30.0%
! ATTCTTATTATTTCTCCAGC+AGG + chr4_3:17241649-17241668 Msa0632800:CDS 30.0%
CTTCAAAATTGTTCTTGCTA+TGG - chr4_3:17241710-17241729 None:intergenic 30.0%
! CACAAAAAATGATGAGCTTT+TGG + chr4_3:17241786-17241805 Msa0632800:CDS 30.0%
AAAATTGGACACATCTTTAG+TGG + chr4_3:17241921-17241940 Msa0632800:CDS 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG + chr4_3:17241961-17241980 Msa0632800:CDS 30.0%
TGTACATCTTCTAATCAACT+TGG + chr4_3:17242009-17242028 Msa0632800:intron 30.0%
AATTTCATAACCGTTGTTGT+TGG - chr4_3:17242188-17242207 None:intergenic 30.0%
AACAACAACGGTTATGAAAT+TGG + chr4_3:17242187-17242206 Msa0632800:CDS 30.0%
! TCCTTTTGGATCATAAATCT+GGG - chr4_3:17242453-17242472 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATC+TGG - chr4_3:17242454-17242473 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG + chr4_3:17242500-17242519 Msa0632800:CDS 30.0%
CTAGCATATCATTCATCTTT+AGG - chr4_3:17242581-17242600 None:intergenic 30.0%
TTGGAATAAGGTGATATAAG+CGG + chr4_3:17242633-17242652 Msa0632800:three_prime_UTR 30.0%
TGAGAAAATAGTTGGAATCA+TGG - chr4_3:17242678-17242697 None:intergenic 30.0%
!! TGGATTTTTATTTGAGTGGT+TGG + chr4_3:17242762-17242781 Msa0632800:three_prime_UTR 30.0%
!!! CTTTGGATTTTGGTTGTATT+GGG + chr4_3:17242819-17242838 Msa0632800:three_prime_UTR 30.0%
! GCATTTTCAAAGAGAAAGTC+TGG + chr4_3:17241574-17241593 Msa0632800:CDS 35.0%
! TAACAATATGGTTTTCCTGC+TGG - chr4_3:17241667-17241686 None:intergenic 35.0%
GCAGGAAAACCATATTGTTA+TGG + chr4_3:17241667-17241686 Msa0632800:CDS 35.0%
GAAGGTTCTCCATAACAATA+TGG - chr4_3:17241679-17241698 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG + chr4_3:17241804-17241823 Msa0632800:CDS 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG + chr4_3:17241844-17241863 Msa0632800:CDS 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG + chr4_3:17241851-17241870 Msa0632800:CDS 35.0%
TATGTGTTAGGTTTATCGCA+TGG - chr4_3:17242397-17242416 None:intergenic 35.0%
TTTCTTGTCATTGAAGCTCA+TGG + chr4_3:17242425-17242444 Msa0632800:intron 35.0%
TCCCAGATTTATGATCCAAA+AGG + chr4_3:17242449-17242468 Msa0632800:CDS 35.0%
GATGTTATTGCTTCTCTTGA+AGG + chr4_3:17242482-17242501 Msa0632800:CDS 35.0%
GCTTATATCACCTTATTCCA+AGG - chr4_3:17242634-17242653 None:intergenic 35.0%
!!! GCTTTGGATTTTGGTTGTAT+TGG + chr4_3:17242818-17242837 Msa0632800:three_prime_UTR 35.0%
! GCATTTTCAAAGAGAAAGTC+TGG + chr4_3:17241574-17241593 Msa0632800:CDS 35.0%
! TAACAATATGGTTTTCCTGC+TGG - chr4_3:17241667-17241686 None:intergenic 35.0%
GCAGGAAAACCATATTGTTA+TGG + chr4_3:17241667-17241686 Msa0632800:CDS 35.0%
GAAGGTTCTCCATAACAATA+TGG - chr4_3:17241679-17241698 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG + chr4_3:17241804-17241823 Msa0632800:CDS 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG + chr4_3:17241844-17241863 Msa0632800:CDS 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG + chr4_3:17241851-17241870 Msa0632800:CDS 35.0%
TATGTGTTAGGTTTATCGCA+TGG - chr4_3:17242397-17242416 None:intergenic 35.0%
TTTCTTGTCATTGAAGCTCA+TGG + chr4_3:17242425-17242444 Msa0632800:intron 35.0%
TCCCAGATTTATGATCCAAA+AGG + chr4_3:17242449-17242468 Msa0632800:CDS 35.0%
GATGTTATTGCTTCTCTTGA+AGG + chr4_3:17242482-17242501 Msa0632800:CDS 35.0%
GCTTATATCACCTTATTCCA+AGG - chr4_3:17242634-17242653 None:intergenic 35.0%
!!! GCTTTGGATTTTGGTTGTAT+TGG + chr4_3:17242818-17242837 Msa0632800:three_prime_UTR 35.0%
! CTTGCTATGGATTGAGAAGA+AGG - chr4_3:17241697-17241716 None:intergenic 40.0%
AGTAGTGTCTTAAGCAATGG+AGG + chr4_3:17241964-17241983 Msa0632800:CDS 40.0%
TCTTGCATAGACGAACTGAA+TGG + chr4_3:17242130-17242149 Msa0632800:CDS 40.0%
ACCAATCCTCCTTATGTCTT+TGG + chr4_3:17242160-17242179 Msa0632800:CDS 40.0%
CCAATCCTCCTTATGTCTTT+GGG + chr4_3:17242161-17242180 Msa0632800:CDS 40.0%
CAATCCTCCTTATGTCTTTG+GGG + chr4_3:17242162-17242181 Msa0632800:CDS 40.0%
TGATCCAAAAGGAAAAGGCA+AGG + chr4_3:17242460-17242479 Msa0632800:CDS 40.0%
! ACATCCTTGCCTTTTCCTTT+TGG - chr4_3:17242467-17242486 None:intergenic 40.0%
CATTTCCACTTTCACTTGCA+CGG - chr4_3:17242538-17242557 None:intergenic 40.0%
ATAAGCGGACATCACATTTC+AGG + chr4_3:17242648-17242667 Msa0632800:three_prime_UTR 40.0%
TAAGCGGACATCACATTTCA+GGG + chr4_3:17242649-17242668 Msa0632800:three_prime_UTR 40.0%
! CTTGCTATGGATTGAGAAGA+AGG - chr4_3:17241697-17241716 None:intergenic 40.0%
AGTAGTGTCTTAAGCAATGG+AGG + chr4_3:17241964-17241983 Msa0632800:CDS 40.0%
TCTTGCATAGACGAACTGAA+TGG + chr4_3:17242130-17242149 Msa0632800:CDS 40.0%
ACCAATCCTCCTTATGTCTT+TGG + chr4_3:17242160-17242179 Msa0632800:CDS 40.0%
CCAATCCTCCTTATGTCTTT+GGG + chr4_3:17242161-17242180 Msa0632800:CDS 40.0%
CAATCCTCCTTATGTCTTTG+GGG + chr4_3:17242162-17242181 Msa0632800:CDS 40.0%
TGATCCAAAAGGAAAAGGCA+AGG + chr4_3:17242460-17242479 Msa0632800:CDS 40.0%
! ACATCCTTGCCTTTTCCTTT+TGG - chr4_3:17242467-17242486 None:intergenic 40.0%
CATTTCCACTTTCACTTGCA+CGG - chr4_3:17242538-17242557 None:intergenic 40.0%
ATAAGCGGACATCACATTTC+AGG + chr4_3:17242648-17242667 Msa0632800:three_prime_UTR 40.0%
TAAGCGGACATCACATTTCA+GGG + chr4_3:17242649-17242668 Msa0632800:three_prime_UTR 40.0%
CTACCTCTTGATGTCCTCTT+CGG - chr4_3:17241505-17241524 None:intergenic 45.0%
AGCACAACGGGAAAGTTTGA+AGG + chr4_3:17241828-17241847 Msa0632800:CDS 45.0%
ACGGGAAAGTTTGAAGGCTT+TGG + chr4_3:17241834-17241853 Msa0632800:CDS 45.0%
AGTGTCTTAAGCAATGGAGG+AGG + chr4_3:17241967-17241986 Msa0632800:CDS 45.0%
GTGTCTTAAGCAATGGAGGA+GGG + chr4_3:17241968-17241987 Msa0632800:CDS 45.0%
GGAGGAGGGATTAATTCTCA+TGG + chr4_3:17241982-17242001 Msa0632800:CDS 45.0%
!! AGACATAAGGAGGATTGGTC+TGG - chr4_3:17242159-17242178 None:intergenic 45.0%
CCCAAAGACATAAGGAGGAT+TGG - chr4_3:17242164-17242183 None:intergenic 45.0%
TTGTTGGCCCCAAAGACATA+AGG - chr4_3:17242172-17242191 None:intergenic 45.0%
CGTGCAAGTGAAAGTGGAAA+TGG + chr4_3:17242536-17242555 Msa0632800:CDS 45.0%
CTACCTCTTGATGTCCTCTT+CGG - chr4_3:17241505-17241524 None:intergenic 45.0%
AGCACAACGGGAAAGTTTGA+AGG + chr4_3:17241828-17241847 Msa0632800:CDS 45.0%
ACGGGAAAGTTTGAAGGCTT+TGG + chr4_3:17241834-17241853 Msa0632800:CDS 45.0%
AGTGTCTTAAGCAATGGAGG+AGG + chr4_3:17241967-17241986 Msa0632800:CDS 45.0%
GTGTCTTAAGCAATGGAGGA+GGG + chr4_3:17241968-17241987 Msa0632800:CDS 45.0%
GGAGGAGGGATTAATTCTCA+TGG + chr4_3:17241982-17242001 Msa0632800:CDS 45.0%
!! AGACATAAGGAGGATTGGTC+TGG - chr4_3:17242159-17242178 None:intergenic 45.0%
CCCAAAGACATAAGGAGGAT+TGG - chr4_3:17242164-17242183 None:intergenic 45.0%
TTGTTGGCCCCAAAGACATA+AGG - chr4_3:17242172-17242191 None:intergenic 45.0%
CGTGCAAGTGAAAGTGGAAA+TGG + chr4_3:17242536-17242555 Msa0632800:CDS 45.0%
! AGCATGTTGCGGAAGCACAA+CGG + chr4_3:17241815-17241834 Msa0632800:CDS 50.0%
TTGGCCCCAAAGACATAAGG+AGG - chr4_3:17242169-17242188 None:intergenic 50.0%
!! GTCTTTGGGGCCAACAACAA+CGG + chr4_3:17242175-17242194 Msa0632800:CDS 50.0%
TCACTTGCACGGTCAAGGTT+TGG - chr4_3:17242527-17242546 None:intergenic 50.0%
CACTTTCACTTGCACGGTCA+AGG - chr4_3:17242532-17242551 None:intergenic 50.0%
CTTGACCGTGCAAGTGAAAG+TGG + chr4_3:17242530-17242549 Msa0632800:CDS 50.0%
! AGCATGTTGCGGAAGCACAA+CGG + chr4_3:17241815-17241834 Msa0632800:CDS 50.0%
TTGGCCCCAAAGACATAAGG+AGG - chr4_3:17242169-17242188 None:intergenic 50.0%
!! GTCTTTGGGGCCAACAACAA+CGG + chr4_3:17242175-17242194 Msa0632800:CDS 50.0%
TCACTTGCACGGTCAAGGTT+TGG - chr4_3:17242527-17242546 None:intergenic 50.0%
CACTTTCACTTGCACGGTCA+AGG - chr4_3:17242532-17242551 None:intergenic 50.0%
CTTGACCGTGCAAGTGAAAG+TGG + chr4_3:17242530-17242549 Msa0632800:CDS 50.0%
GCTCCGAAGAGGACATCAAG+AGG + chr4_3:17241499-17241518 Msa0632800:CDS 55.0%
! GCATGTTGCGGAAGCACAAC+GGG + chr4_3:17241816-17241835 Msa0632800:CDS 55.0%
GCGGACATCACATTTCAGGG+TGG + chr4_3:17242652-17242671 Msa0632800:three_prime_UTR 55.0%
GCTCCGAAGAGGACATCAAG+AGG + chr4_3:17241499-17241518 Msa0632800:CDS 55.0%
! GCATGTTGCGGAAGCACAAC+GGG + chr4_3:17241816-17241835 Msa0632800:CDS 55.0%
GCGGACATCACATTTCAGGG+TGG + chr4_3:17242652-17242671 Msa0632800:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 17241496 17242842 17241496 ID=Msa0632800;Name=Msa0632800
chr4_3 mRNA 17241496 17242842 17241496 ID=Msa0632800-mRNA-1;Parent=Msa0632800;Name=Msa0632800-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0.5|0.33|1|1|1|3|250|249
chr4_3 exon 17241496 17242003 17241496 ID=Msa0632800-mRNA-1:exon:4821;Parent=Msa0632800-mRNA-1
chr4_3 exon 17242125 17242214 17242125 ID=Msa0632800-mRNA-1:exon:4822;Parent=Msa0632800-mRNA-1
chr4_3 exon 17242441 17242842 17242441 ID=Msa0632800-mRNA-1:exon:4823;Parent=Msa0632800-mRNA-1
chr4_3 CDS 17241496 17242003 17241496 ID=Msa0632800-mRNA-1:cds;Parent=Msa0632800-mRNA-1
chr4_3 CDS 17242125 17242214 17242125 ID=Msa0632800-mRNA-1:cds;Parent=Msa0632800-mRNA-1
chr4_3 CDS 17242441 17242592 17242441 ID=Msa0632800-mRNA-1:cds;Parent=Msa0632800-mRNA-1
chr4_3 three_prime_UTR 17242593 17242842 17242593 ID=Msa0632800-mRNA-1:three_prime_utr;Parent=Msa0632800-mRNA-1
Gene Sequence

>Msa0632800

ATGGCTCCGAAGAGGACATCAAGAGGTAGACAAAAAAGAGAAATGAAGAAGATTGAGAGAGAAAGCGATCTTTTAGTAGCATTTTCAAAGAGAAAGTCTGGCATTTACAACAAAGCCTCTGAATTATCAACACTTTGTGGGGCTTTAGTTGATATTCTTATTATTTCTCCAGCAGGAAAACCATATTGTTATGGAGAACCTTCTTCTCAATCCATAGCAAGAACAATTTTGAAGAATGAAAACCCTTCAGTAGAAGAAGAATTAAAGCAACTAAATATTAACCAAGTTAACACAAAAAATGATGAGCTTTTGGATGAAAAGCATGTTGCGGAAGCACAACGGGAAAGTTTGAAGGCTTTGGAAAGTTATGGCTATTGGTCGATCAATGAGAAAGATGTTGAAAGCTTTGATTATGACCAGTTAAAAAAATTGGACACATCTTTAGTGGACATTGCAAATAAATTGATAAGTAGTGTCTTAAGCAATGGAGGAGGGATTAATTCTCATGCAATTTCTTGCATAGACGAACTGAATGGAGTCCAGACCAATCCTCCTTATGTCTTTGGGGCCAACAACAACGGTTATGAAATTGGTGCAACTCATGGATCCCAGATTTATGATCCAAAAGGAAAAGGCAAGGATGTTATTGCTTCTCTTGAAGGTAGAAATAAAACACAAGGACCAAACCTTGACCGTGCAAGTGAAAGTGGAAATGGAAGTGATGTTGATCATTCTCCTAAAGATGAATGA
Protein sequence

>Msa0632800

MAPKRTSRGRQKREMKKIERESDLLVAFSKRKSGIYNKASELSTLCGALVDILIISPAGKPYCYGEPSSQSIARTILKNENPSVEEELKQLNINQVNTKNDELLDEKHVAEAQRESLKALESYGYWSINEKDVESFDYDQLKKLDTSLVDIANKLISSVLSNGGGINSHAISCIDELNGVQTNPPYVFGANNNGYEIGATHGSQIYDPKGKGKDVIASLEGRNKTQGPNLDRASESGNGSDVDHSPKDE*