AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0633830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0633830 MtrunA17_Chr4g0012071 95.131 534 24 2 93 625 20 552 0.0 1025
Msa0633830 MtrunA17_Chr7g0230771 61.290 434 152 5 199 625 308 732 1.06e-169 501
Msa0633830 MtrunA17_Chr7g0230771 35.610 205 102 9 1 189 4 194 1.12e-12 71.2
Msa0633830 MtrunA17_Chr7g0230731 60.599 434 155 5 199 625 305 729 1.77e-166 493
Msa0633830 MtrunA17_Chr8g0373561 33.784 370 227 10 267 625 180 542 1.64e-53 191
Msa0633830 MtrunA17_Chr8g0376381 25.967 362 252 6 270 624 170 522 1.51e-32 132
Msa0633830 MtrunA17_Chr4g0038761 28.571 371 228 11 274 624 89 442 1.53e-32 130
Msa0633830 MtrunA17_Chr2g0306641 26.812 414 258 12 231 625 101 488 2.40e-32 130
Msa0633830 MtrunA17_Chr7g0224271 28.169 426 255 15 230 625 270 674 3.30e-32 132
Msa0633830 MtrunA17_Chr5g0445331 27.321 377 246 10 270 624 162 532 1.49e-31 129
Msa0633830 MtrunA17_Chr4g0039661 29.897 388 221 15 266 624 190 555 6.76e-31 127
Msa0633830 MtrunA17_Chr3g0110971 25.995 377 248 8 270 624 220 587 1.72e-30 126
Msa0633830 MtrunA17_Chr3g0101141 26.042 384 242 12 268 624 174 542 2.51e-30 125
Msa0633830 MtrunA17_Chr5g0447691 24.865 370 260 8 270 624 178 544 1.29e-29 123
Msa0633830 MtrunA17_Chr7g0245601 26.829 369 233 8 274 624 443 792 2.16e-28 120
Msa0633830 MtrunA17_Chr2g0290241 25.594 379 253 11 268 625 56 426 1.16e-27 116
Msa0633830 MtrunA17_Chr2g0319091 24.083 436 280 12 223 623 159 578 5.86e-26 112
Msa0633830 MtrunA17_Chr4g0057291 25.789 380 245 11 270 623 293 661 3.81e-25 110
Msa0633830 MtrunA17_Chr3g0125811 25.806 372 254 8 270 624 204 570 1.94e-24 107
Msa0633830 MtrunA17_Chr4g0076091 24.802 379 252 10 270 625 176 544 2.61e-24 107
Msa0633830 MtrunA17_Chr1g0183641 25.000 364 234 11 283 625 133 478 3.50e-24 106
Msa0633830 MtrunA17_Chr1g0159461 25.798 376 249 10 270 624 226 592 8.13e-24 106
Msa0633830 MtrunA17_Chr5g0403741 27.778 234 151 8 401 625 51 275 3.71e-22 97.1
Msa0633830 MtrunA17_Chr5g0424131 25.490 408 263 15 250 625 102 500 8.42e-22 99.4
Msa0633830 MtrunA17_Chr4g0057891 24.937 397 260 13 256 625 136 521 4.89e-21 97.1
Msa0633830 MtrunA17_Chr7g0270031 23.776 429 291 10 228 624 130 554 8.88e-21 96.3
Msa0633830 MtrunA17_Chr3g0114841 27.575 301 201 10 337 625 206 501 1.88e-20 95.1
Msa0633830 MtrunA17_Chr2g0328381 25.067 375 250 12 270 623 276 640 4.19e-19 91.7
Msa0633830 MtrunA17_Chr8g0383981 26.963 382 239 16 263 623 133 495 6.65e-19 90.5
Msa0633830 MtrunA17_Chr2g0328431 22.464 414 288 10 232 623 251 653 1.63e-18 89.7
Msa0633830 MtrunA17_Chr2g0294471 25.459 381 259 12 265 624 200 576 3.29e-18 88.6
Msa0633830 MtrunA17_Chr7g0238891 21.622 407 275 10 248 624 348 740 1.50e-17 86.7
Msa0633830 MtrunA17_Chr2g0294461 25.260 384 259 12 265 624 206 585 2.13e-17 85.9
Msa0633830 MtrunA17_Chr2g0328341 25.455 275 183 7 270 531 273 538 6.42e-17 84.7
Msa0633830 MtrunA17_Chr2g0328391 22.312 372 260 8 274 623 304 668 8.85e-17 84.3
Msa0633830 MtrunA17_Chr2g0328411 21.939 392 274 10 251 623 275 653 3.84e-16 82.0
Msa0633830 MtrunA17_Chr8g0357601 22.166 397 266 10 258 624 169 552 4.36e-15 78.6
Msa0633830 MtrunA17_Chr5g0401131 20.627 383 269 9 270 624 115 490 6.44e-15 77.8
Msa0633830 MtrunA17_Chr4g0031741 22.281 377 258 10 270 623 285 649 9.13e-15 77.8
Msa0633830 MtrunA17_Chr4g0031711 25.455 330 215 10 219 529 316 633 2.12e-14 76.6
Msa0633830 MtrunA17_Chr2g0328361 23.514 370 237 14 270 623 275 614 3.23e-14 75.9
Msa0633830 MtrunA17_Chr1g0194621 23.860 285 199 8 349 624 243 518 3.57e-14 75.5
Msa0633830 MtrunA17_Chr2g0328371 26.601 203 134 5 337 531 2 197 9.06e-14 71.6
Msa0633830 MtrunA17_Chr4g0031701 25.271 277 185 7 270 529 255 526 2.22e-13 73.2
Msa0633830 MtrunA17_Chr2g0329591 21.951 369 246 10 288 624 93 451 2.63e-13 72.4
Msa0633830 MtrunA17_Chr4g0031731 24.910 277 186 7 270 529 255 526 4.04e-13 72.4
Msa0633830 MtrunA17_Chr2g0328401 22.973 222 153 6 229 440 250 463 1.37e-12 70.9
Msa0633830 MtrunA17_Chr4g0031721 18.660 418 304 11 234 623 274 683 7.98e-12 68.6
Msa0633830 MtrunA17_Chr5g0396491 27.746 173 117 3 270 438 57 225 5.83e-11 65.1
Msa0633830 MtrunA17_Chr4g0038851 31.351 185 113 4 270 446 49 227 8.10e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0633830 AT4G00150.1 42.381 630 280 18 4 625 1 555 2.59e-135 407
Msa0633830 AT2G45160.1 39.435 672 325 22 4 625 1 640 1.39e-110 346
Msa0633830 AT3G60630.1 39.848 660 322 24 4 625 1 623 1.20e-109 343
Msa0633830 AT4G36710.1 32.713 376 220 11 268 624 124 485 1.78e-52 188
Msa0633830 AT2G04890.1 27.957 372 239 9 270 624 54 413 2.90e-36 141
Msa0633830 AT5G66770.1 28.497 386 251 13 256 624 207 584 5.26e-35 140
Msa0633830 AT2G01570.1 26.902 368 245 9 270 624 225 581 3.75e-30 125
Msa0633830 AT5G17490.1 27.989 368 240 10 270 624 161 516 8.42e-30 124
Msa0633830 AT3G03450.1 25.410 366 258 6 270 624 184 545 2.66e-29 122
Msa0633830 AT5G48150.1 23.873 377 270 8 263 624 116 490 3.52e-29 122
Msa0633830 AT5G48150.4 23.873 377 270 8 263 624 116 490 3.52e-29 122
Msa0633830 AT5G48150.2 23.873 377 270 8 263 624 116 490 3.52e-29 122
Msa0633830 AT5G48150.3 23.873 377 270 8 263 624 116 490 3.52e-29 122
Msa0633830 AT3G50650.1 27.649 387 239 12 262 624 173 542 3.73e-29 122
Msa0633830 AT3G54220.1 26.923 364 240 7 274 623 298 649 4.67e-29 123
Msa0633830 AT4G17230.1 26.772 381 253 10 263 624 152 525 1.34e-28 120
Msa0633830 AT4G17230.2 26.772 381 253 10 263 624 152 525 1.34e-28 120
Msa0633830 AT1G66350.1 25.272 368 247 10 270 625 156 507 4.75e-28 119
Msa0633830 AT1G14920.1 25.549 364 255 8 270 624 173 529 6.01e-28 118
Msa0633830 AT1G50600.3 23.628 419 263 11 236 624 135 526 3.17e-27 116
Msa0633830 AT1G21450.1 24.865 370 261 7 270 624 226 593 5.33e-27 116
Msa0633830 AT1G50600.1 23.628 419 263 11 236 624 206 597 9.84e-27 115
Msa0633830 AT1G50600.2 23.628 419 263 11 236 624 210 601 1.28e-26 115
Msa0633830 AT5G41920.1 25.401 374 243 9 270 624 44 400 4.84e-26 111
Msa0633830 AT1G63100.2 25.849 383 238 13 275 623 283 653 5.07e-22 101
Msa0633830 AT1G63100.1 25.849 383 238 13 275 623 258 628 5.12e-22 101
Msa0633830 AT4G08250.1 26.246 301 203 10 337 623 181 476 5.93e-19 90.9
Msa0633830 AT5G59450.1 22.252 373 270 7 270 624 228 598 8.21e-19 90.9
Msa0633830 AT1G55580.1 24.918 305 214 6 335 624 141 445 9.84e-19 89.7
Msa0633830 AT4G37650.1 20.052 384 277 8 270 624 147 529 4.55e-16 82.0
Msa0633830 AT3G46600.1 22.372 371 268 8 270 624 213 579 1.81e-15 80.1
Msa0633830 AT1G07530.1 20.773 414 309 10 229 623 351 764 3.12e-15 79.7
Msa0633830 AT3G46600.2 22.372 371 268 8 270 624 5 371 4.16e-15 77.8
Msa0633830 AT3G46600.3 22.372 371 268 8 270 624 181 547 4.50e-15 79.0
Msa0633830 AT1G07520.1 26.238 202 138 5 274 466 323 522 5.91e-14 75.9
Msa0633830 AT1G07520.2 26.238 202 138 5 274 466 159 358 6.37e-14 75.1
Msa0633830 AT1G07520.4 26.238 202 138 5 274 466 159 358 6.37e-14 75.1
Msa0633830 AT1G07520.3 26.238 202 138 5 274 466 324 523 6.80e-14 75.5
Msa0633830 AT1G50420.1 29.016 193 120 4 270 449 58 246 2.77e-12 69.7
Msa0633830 AT2G29060.1 21.448 373 271 10 270 623 322 691 5.42e-12 69.3
Msa0633830 AT2G29060.2 21.448 373 271 10 270 623 285 654 6.88e-12 68.9
Msa0633830 AT2G29065.1 21.658 374 266 10 274 623 257 627 9.52e-12 68.6

Find 156 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAAGAATCCTCTCTTTA+TGG 0.092963 4_3:+19985149 Msa0633830:three_prime_UTR
CCTAATGTTTGTTAACTAAA+TGG 0.126757 4_3:+19984985 Msa0633830:three_prime_UTR
TTACACAACAATACACTTTA+AGG 0.131559 4_3:+19982533 Msa0633830:five_prime_UTR
AAAATAAAGATTGAGTATTT+TGG 0.160504 4_3:-19982251 None:intergenic
TGTAATGCCTTTCAATCTTT+TGG 0.183368 4_3:-19984606 None:intergenic
AATTGCGTGCTGAGATATTT+TGG 0.187631 4_3:-19982342 None:intergenic
CATGTTATTGATTTCGATAT+TGG 0.238642 4_3:+19984112 Msa0633830:CDS
GTCCTGTCTTTCTTGATCTA+AGG 0.250421 4_3:+19985176 Msa0633830:three_prime_UTR
GATAAGTGGCTAATTATTAA+AGG 0.259137 4_3:+19984875 Msa0633830:exon
AGACAAGGCATAGTAATTTA+TGG 0.269109 4_3:-19982116 None:intergenic
GTGTTACCGCTCTTGGATTC+TGG 0.279830 4_3:+19983590 Msa0633830:CDS
TTGAAATTCCTCAAAAGTTA+AGG 0.300516 4_3:-19983012 None:intergenic
CCATTTAGTTAACAAACATT+AGG 0.311269 4_3:-19984985 None:intergenic
ATGTTATTGATTTCGATATT+GGG 0.325426 4_3:+19984113 Msa0633830:CDS
GACAATGTTGAAGATGAAAA+TGG 0.331833 4_3:-19983200 None:intergenic
TCCAAAGTTACGACCACTTT+CGG 0.339309 4_3:-19984469 None:intergenic
ATTGTTTATAGAAACTGATT+TGG 0.341228 4_3:-19982148 None:intergenic
TTAAAGGTTGAAATTTCTTA+AGG 0.345571 4_3:+19984891 Msa0633830:three_prime_UTR
TTGCAATGTGTTACCGCTCT+TGG 0.347688 4_3:+19983583 Msa0633830:CDS
TGATGGATTACTTATGAAAC+TGG 0.357205 4_3:-19984407 None:intergenic
ATTGATTTCGATATTGGGTT+TGG 0.358381 4_3:+19984118 Msa0633830:CDS
CATTTAGTTAACAAACATTA+GGG 0.374065 4_3:-19984984 None:intergenic
CTTCCTCTCTTGTGCTATGC+TGG 0.380612 4_3:+19984812 Msa0633830:CDS
CTAATGTTTGTTAACTAAAT+GGG 0.381359 4_3:+19984986 Msa0633830:three_prime_UTR
ACAATAATAGAAACTAATTA+TGG 0.382308 4_3:-19982291 None:intergenic
TGAAATTCCTCAAAAGTTAA+GGG 0.383228 4_3:-19983011 None:intergenic
TCTTGATCTAAGGTTGTGTT+TGG 0.383941 4_3:+19985186 Msa0633830:three_prime_UTR
GGTTCAAGCGCAAGGGATAT+TGG 0.384808 4_3:+19983844 Msa0633830:CDS
ATCAACCTCAGCAACAAAAT+TGG 0.386235 4_3:+19983105 Msa0633830:CDS
ACTAATTATGGTTATGATAA+GGG 0.386415 4_3:-19982279 None:intergenic
TAAACACTCGATCGAGTGTT+TGG 0.388594 4_3:+19985240 Msa0633830:three_prime_UTR
TGAATGAAGCATTAACTGTA+AGG 0.398966 4_3:-19983936 None:intergenic
TAGTAAGAAATGAACAGTTT+AGG 0.403129 4_3:+19985121 Msa0633830:three_prime_UTR
AACTAATTATGGTTATGATA+AGG 0.416365 4_3:-19982280 None:intergenic
AGCTGAGTGTTTGGTTCAAA+GGG 0.419565 4_3:+19984756 Msa0633830:CDS
AATTAGCTGTAATTTGTGTT+TGG 0.426950 4_3:+19985294 Msa0633830:three_prime_UTR
GGAAGCTCAAGCTGAGTGTT+TGG 0.430829 4_3:+19984747 Msa0633830:CDS
CAAATATACTCTGCTGAAGC+TGG 0.434387 4_3:-19983778 None:intergenic
ATTTCAAGTGAAGGGAATGT+TGG 0.436223 4_3:+19983028 Msa0633830:CDS
TTACTTATGAAACTGGAAAC+AGG 0.436478 4_3:-19984400 None:intergenic
AATATCCCTTGCGCTTGAAC+CGG 0.438162 4_3:-19983842 None:intergenic
GCCGAAAGTGGTCGTAACTT+TGG 0.444136 4_3:+19984468 Msa0633830:CDS
CTTTGAAAAGGCTTACCAAT+TGG 0.446191 4_3:-19983890 None:intergenic
TCAAGCCCTCATTGAAGCCT+TGG 0.450274 4_3:+19984072 Msa0633830:CDS
AAGCTGAGTGTTTGGTTCAA+AGG 0.455607 4_3:+19984755 Msa0633830:CDS
GAATGTGAAGGGAGAGAAAC+CGG 0.460030 4_3:-19984713 None:intergenic
TTGCAATTCTCAGGAATGAA+TGG 0.464532 4_3:-19983566 None:intergenic
TGGTATGAGAATGGAGGATT+GGG 0.465608 4_3:+19983343 Msa0633830:CDS
TAGAAAGAAGCCCTTTGAAA+AGG 0.471306 4_3:-19983902 None:intergenic
AAGTTGGCAAACTGAAGAAC+AGG 0.472756 4_3:-19984040 None:intergenic
AACTCATCTCAGTTTCAACT+TGG 0.473456 4_3:+19984845 Msa0633830:CDS
CTTCACATTCAGCAAATTCA+CGG 0.474423 4_3:+19984726 Msa0633830:CDS
GAAGATGAAAATGGAGAACT+AGG 0.474745 4_3:-19983191 None:intergenic
AAGGTTGAAATTTCTTAAGG+TGG 0.475998 4_3:+19984894 Msa0633830:three_prime_UTR
ATGGTTGAACATGGACGCTT+CGG 0.477095 4_3:+19983517 Msa0633830:CDS
AAAGAACAAGGACAAAGCTT+AGG 0.481534 4_3:-19982181 None:intergenic
AATCACCAGCTCTCACCAAT+TGG 0.485962 4_3:+19983875 Msa0633830:CDS
AGTTAACAAACATTAGGGTA+TGG 0.486346 4_3:-19984979 None:intergenic
AAGGGCTTCTTTCTACATGA+AGG 0.487466 4_3:+19983910 Msa0633830:CDS
CTTGCCATCATGTCCTCTAC+CGG 0.490539 4_3:+19984336 Msa0633830:CDS
GGCTTGATTACAAGTAAAGT+TGG 0.491191 4_3:-19984056 None:intergenic
CAGTTAATGCTTCATTCAAA+CGG 0.491869 4_3:+19983941 Msa0633830:CDS
TGGTGGTGGTGGTATGAGAA+TGG 0.492769 4_3:+19983334 Msa0633830:CDS
TTTGAGGAATTTCAAGTGAA+GGG 0.497110 4_3:+19983020 Msa0633830:CDS
GCTGATAGAAGCTGGTAATC+CGG 0.498189 4_3:+19983823 Msa0633830:CDS
AGGAACATGTTTCTTCAGTC+CGG 0.498389 4_3:+19984694 Msa0633830:CDS
GGTAACACATTGCAATTCTC+AGG 0.500098 4_3:-19983575 None:intergenic
GTGGTATGAGAATGGAGGAT+TGG 0.504718 4_3:+19983342 Msa0633830:CDS
TGCTCAGATATCAATGAGGC+AGG 0.504902 4_3:-19982204 None:intergenic
TTCAAATAGCAATACTTCAA+TGG 0.504902 4_3:+19983256 Msa0633830:CDS
TTTGATAGTAATGAAGCCAT+TGG 0.505073 4_3:+19984367 Msa0633830:CDS
CAACCGACGACTGTTGTTGT+TGG 0.506049 4_3:-19983697 None:intergenic
GTAATTTGTCACGCTGGTTG+TGG 0.506339 4_3:-19984663 None:intergenic
CTGAAGAACAGGTGATATCT+CGG 0.507582 4_3:-19984029 None:intergenic
TCCAATTGCGAAGCAGCAAA+AGG 0.508239 4_3:+19983721 Msa0633830:CDS
GATGTTGAAGACCCTTATGC+TGG 0.508840 4_3:+19983404 Msa0633830:CDS
TGTTTGTTAACTAAATGGGT+TGG 0.510170 4_3:+19984990 Msa0633830:three_prime_UTR
TCTTGAATCAAGAACTGAAG+TGG 0.511894 4_3:-19983162 None:intergenic
AACTTGGAGATGTTGATAAG+TGG 0.518300 4_3:+19984861 Msa0633830:exon
TCCACTTGAAATCCTCTCAC+AGG 0.520356 4_3:-19984781 None:intergenic
AACCTTAGATCAAGAAAGAC+AGG 0.524170 4_3:-19985178 None:intergenic
CTGGTCAAGAAGTGTTTGCT+AGG 0.526401 4_3:+19983609 Msa0633830:CDS
CTCAATCTCGTTGCAAGACG+AGG 0.528947 4_3:-19984224 None:intergenic
TATGATGTCTCTTTGAACAT+TGG 0.534220 4_3:+19985042 Msa0633830:three_prime_UTR
CTGGATGCTTGCATCATCTC+CGG 0.537282 4_3:-19983759 None:intergenic
TGAACGACATTGGTGGGAAG+AGG 0.544276 4_3:-19984517 None:intergenic
TTGTCATCTTCTCATGGTAG+TGG 0.544818 4_3:+19983218 Msa0633830:CDS
ATCTTGCAAAGTGGTGGTTG+TGG 0.551252 4_3:+19983437 Msa0633830:CDS
TGCTAGGAGACATCAGCAGC+AGG 0.551740 4_3:+19983625 Msa0633830:CDS
GGTAATCCGGTTCAAGCGCA+AGG 0.552039 4_3:+19983836 Msa0633830:CDS
TCAACATTGTCATCTTCTCA+TGG 0.552805 4_3:+19983212 Msa0633830:CDS
CTCTTGTGCTATGCTGGCAG+AGG 0.553514 4_3:+19984818 Msa0633830:CDS
GGTAGTGGTAGTAATGGTAA+TGG 0.556024 4_3:+19983233 Msa0633830:CDS
GCTTACCAATTGGTGAGAGC+TGG 0.557459 4_3:-19983880 None:intergenic
AAGGTGAGTTCTAGTACTAC+CGG 0.560768 4_3:+19983740 Msa0633830:CDS
ACTGCTGAGCTGATAGAAGC+TGG 0.561856 4_3:+19983815 Msa0633830:CDS
AAAGCAGCTAAGGCCGAAAG+TGG 0.562649 4_3:+19984456 Msa0633830:CDS
CCTCTCCAAGGCTTCAATGA+GGG 0.564144 4_3:-19984077 None:intergenic
CAGCATAGCACAAGAGAGGA+AGG 0.564647 4_3:-19984811 None:intergenic
ATGTACTCCAAAAGATTGAA+AGG 0.570089 4_3:+19984599 Msa0633830:CDS
GTTCAAAGGGCACCTGTGAG+AGG 0.570717 4_3:+19984769 Msa0633830:CDS
AGAGCCACAGGGAAGCATGA+TGG 0.571215 4_3:-19984424 None:intergenic
CATCATTTGAAAGATGAGCA+AGG 0.573001 4_3:-19983292 None:intergenic
TCTCATGGTAGTGGTAGTAA+TGG 0.576556 4_3:+19983227 Msa0633830:CDS
CGTGAATTTGCTGAATGTGA+AGG 0.578961 4_3:-19984725 None:intergenic
TGAAGAAACATGTTCCTCCA+TGG 0.581165 4_3:-19984688 None:intergenic
ACCATCAACAAAATCGTCCT+CGG 0.581686 4_3:+19984637 Msa0633830:CDS
TCAAAGAAATGACCCGGTAG+AGG 0.583745 4_3:-19984349 None:intergenic
AGAACATGAACGACATTGGT+GGG 0.584347 4_3:-19984523 None:intergenic
CTTGAATCAAGAACTGAAGT+GGG 0.584678 4_3:-19983161 None:intergenic
TGGATCATCAACAACAACAA+TGG 0.586807 4_3:+19983498 Msa0633830:CDS
AACAAAATCTTGCAAAGTGG+TGG 0.589673 4_3:+19983431 Msa0633830:CDS
AAGAACATGAACGACATTGG+TGG 0.589769 4_3:-19984524 None:intergenic
TGGGAAGAGGAACATCCATT+CGG 0.591209 4_3:-19984504 None:intergenic
GTGAATTTGCTGAATGTGAA+GGG 0.591860 4_3:-19984724 None:intergenic
TTCAAACGGTAACAATCTCA+CGG 0.592949 4_3:+19983955 Msa0633830:CDS
AACAGGAAAGTTCACACCAA+TGG 0.593516 4_3:-19984383 None:intergenic
CCCTCATTGAAGCCTTGGAG+AGG 0.594395 4_3:+19984077 Msa0633830:CDS
TTGAAGAACATGAACGACAT+TGG 0.594485 4_3:-19984527 None:intergenic
AGCGTGACAAATTACCTCCA+TGG 0.597025 4_3:+19984671 Msa0633830:CDS
GAGATTGTACTAAGAAGTAA+CGG 0.599321 4_3:+19984166 Msa0633830:CDS
CAATGAGGCAGGAAAGAACA+AGG 0.601819 4_3:-19982193 None:intergenic
GATAACAGAGAAACAGTGAA+AGG 0.602580 4_3:-19985083 None:intergenic
ACCTCTCCAAGGCTTCAATG+AGG 0.608874 4_3:-19984078 None:intergenic
TGGTGGTGGTATGAGAATGG+AGG 0.610885 4_3:+19983337 Msa0633830:CDS
TAAAGAAGATTATTGCTATG+TGG 0.612360 4_3:+19983136 Msa0633830:CDS
ACAACAACAATGGTTGAACA+TGG 0.613222 4_3:+19983508 Msa0633830:CDS
TTGCCATCATGTCCTCTACC+GGG 0.614713 4_3:+19984337 Msa0633830:CDS
TGACCCGGTAGAGGACATGA+TGG 0.615427 4_3:-19984340 None:intergenic
TCATGATGATGAGAAAACAA+TGG 0.623858 4_3:-19983659 None:intergenic
AATTCGATCGAACCTCTCCA+AGG 0.628009 4_3:-19984089 None:intergenic
ATGAGAATGGAGGATTGGGA+AGG 0.628687 4_3:+19983347 Msa0633830:CDS
ATGGAGGTAATTTGTCACGC+TGG 0.629161 4_3:-19984669 None:intergenic
AAGATGAAAATGGAGAACTA+GGG 0.633076 4_3:-19983190 None:intergenic
ACAGCAGTAATCTTAAGTGA+AGG 0.636223 4_3:-19984193 None:intergenic
GTAATCCGGTTCAAGCGCAA+GGG 0.638818 4_3:+19983837 Msa0633830:CDS
CTGCCAGCATAGCACAAGAG+AGG 0.640951 4_3:-19984815 None:intergenic
TCAATCTCGTTGCAAGACGA+GGG 0.646604 4_3:-19984223 None:intergenic
AAGCGCAAGGGATATTGGCG+CGG 0.648472 4_3:+19983849 Msa0633830:CDS
AGATGGATGCGTGCATACGT+AGG 0.650132 4_3:-19982371 None:intergenic
TGAACTCAAACGACATGTTG+AGG 0.652289 4_3:-19984288 None:intergenic
ACCTGTGAGAGGATTTCAAG+TGG 0.654432 4_3:+19984780 Msa0633830:CDS
ATATTGGGTTTGGAGTACAA+TGG 0.656630 4_3:+19984128 Msa0633830:CDS
CAGGACTAAACCATAAAGAG+AGG 0.657124 4_3:-19985159 None:intergenic
ATGGACGCTTCGGTTCAACA+AGG 0.660128 4_3:+19983527 Msa0633830:CDS
TTACTATCAAAGAAATGACC+CGG 0.669847 4_3:-19984355 None:intergenic
TGGACGCTTCGGTTCAACAA+GGG 0.687015 4_3:+19983528 Msa0633830:CDS
CACGCTGGTTGTGGTGACCG+AGG 0.687932 4_3:-19984654 None:intergenic
ATTTGGACATGTCAGAGACA+AGG 0.688777 4_3:-19982131 None:intergenic
TTACCAACAACAACAGTCGT+CGG 0.695811 4_3:+19983694 Msa0633830:CDS
AGAAACATGTTCCTCCATGG+AGG 0.701239 4_3:-19984685 None:intergenic
AAAATGCTCAGATATCAATG+AGG 0.702130 4_3:-19982208 None:intergenic
TAATCCATCATGCTTCCCTG+TGG 0.706732 4_3:+19984420 Msa0633830:CDS
TTGAACAAAATCTTGCAAAG+TGG 0.719051 4_3:+19983428 Msa0633830:CDS
TCTTGACCAGAATCCAAGAG+CGG 0.742204 4_3:-19983596 None:intergenic
GTGACAAATTACCTCCATGG+AGG 0.762900 4_3:+19984674 Msa0633830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGATGATTTTTTTTATT+GGG - chr4_3:19982665-19982684 None:intergenic 10.0%
!!! AAAAAGATGATTTTTTTTAT+TGG - chr4_3:19982666-19982685 None:intergenic 10.0%
!!! AAAATAAAGATTGAGTATTT+TGG - chr4_3:19982254-19982273 None:intergenic 15.0%
!! ACAATAATAGAAACTAATTA+TGG - chr4_3:19982294-19982313 None:intergenic 15.0%
!!! AAAGATGATTTTTTTTATTG+GGG - chr4_3:19982664-19982683 None:intergenic 15.0%
!!! AAAAATCATCTTTTTTCATA+AGG + chr4_3:19982671-19982690 Msa0633830:intron 15.0%
!!! AAAATCATCTTTTTTCATAA+GGG + chr4_3:19982672-19982691 Msa0633830:intron 15.0%
!!! ATTTTGAAAATGAAACTAAT+CGG - chr4_3:19983989-19984008 None:intergenic 15.0%
!!! ATTAGTTTCATTTTCAAAAT+CGG + chr4_3:19983989-19984008 Msa0633830:CDS 15.0%
!!! ATTGTTTGTGTAATTTTTTT+AGG + chr4_3:19984942-19984961 Msa0633830:three_prime_UTR 15.0%
!! ATTGTTTATAGAAACTGATT+TGG - chr4_3:19982151-19982170 None:intergenic 20.0%
!! ACTAATTATGGTTATGATAA+GGG - chr4_3:19982282-19982301 None:intergenic 20.0%
!! AACTAATTATGGTTATGATA+AGG - chr4_3:19982283-19982302 None:intergenic 20.0%
!!! TTTTTTTCTTTTAGCCAAAT+GGG - chr4_3:19982762-19982781 None:intergenic 20.0%
!!! TTTTTTTTCTTTTAGCCAAA+TGG - chr4_3:19982763-19982782 None:intergenic 20.0%
!!! TTGAAATTAAATCTGTTTTC+TGG + chr4_3:19982806-19982825 Msa0633830:intron 20.0%
!!! TGAAATTAAATCTGTTTTCT+GGG + chr4_3:19982807-19982826 Msa0633830:intron 20.0%
!!! TTGTTTTTGTTTTTAGATGA+AGG + chr4_3:19982983-19983002 Msa0633830:intron 20.0%
!!! ATGTTATTGATTTCGATATT+GGG + chr4_3:19984113-19984132 Msa0633830:CDS 20.0%
!! TTAAAGGTTGAAATTTCTTA+AGG + chr4_3:19984891-19984910 Msa0633830:three_prime_UTR 20.0%
!! CATTTAGTTAACAAACATTA+GGG - chr4_3:19984987-19985006 None:intergenic 20.0%
!!! CTAATGTTTGTTAACTAAAT+GGG + chr4_3:19984986-19985005 Msa0633830:three_prime_UTR 20.0%
! TTACACAACAATACACTTTA+AGG + chr4_3:19982533-19982552 Msa0633830:five_prime_UTR 25.0%
!! GAAATTAAATCTGTTTTCTG+GGG + chr4_3:19982808-19982827 Msa0633830:intron 25.0%
! TTCTTTGTTCTTGTTCAAAT+TGG + chr4_3:19982949-19982968 Msa0633830:intron 25.0%
! TGAAATTCCTCAAAAGTTAA+GGG - chr4_3:19983014-19983033 None:intergenic 25.0%
! TTGAAATTCCTCAAAAGTTA+AGG - chr4_3:19983015-19983034 None:intergenic 25.0%
! TAAAGAAGATTATTGCTATG+TGG + chr4_3:19983136-19983155 Msa0633830:CDS 25.0%
! TTCAAATAGCAATACTTCAA+TGG + chr4_3:19983256-19983275 Msa0633830:CDS 25.0%
!!! TCACTTTTGAGGTTAATTAT+GGG + chr4_3:19983380-19983399 Msa0633830:CDS 25.0%
!!! TCTCAAAATGTTGATTTTCA+TGG + chr4_3:19983461-19983480 Msa0633830:CDS 25.0%
!!! CATGTTATTGATTTCGATAT+TGG + chr4_3:19984112-19984131 Msa0633830:CDS 25.0%
!!! ATACTGACTCAAATTTTCTT+GGG - chr4_3:19984260-19984279 None:intergenic 25.0%
!!! TTCATTTTTTAAAGCAGCTA+AGG + chr4_3:19984446-19984465 Msa0633830:CDS 25.0%
! GATAAGTGGCTAATTATTAA+AGG + chr4_3:19984875-19984894 Msa0633830:exon 25.0%
! CCATTTAGTTAACAAACATT+AGG - chr4_3:19984988-19985007 None:intergenic 25.0%
!! CCTAATGTTTGTTAACTAAA+TGG + chr4_3:19984985-19985004 Msa0633830:three_prime_UTR 25.0%
! TAGTAAGAAATGAACAGTTT+AGG + chr4_3:19985121-19985140 Msa0633830:three_prime_UTR 25.0%
! AATTAAGAATCCTCTCTTTA+TGG + chr4_3:19985149-19985168 Msa0633830:three_prime_UTR 25.0%
AGACAAGGCATAGTAATTTA+TGG - chr4_3:19982119-19982138 None:intergenic 30.0%
AAAATGCTCAGATATCAATG+AGG - chr4_3:19982211-19982230 None:intergenic 30.0%
!! GTGTTGTGTTTTTGTTAAGA+TGG - chr4_3:19982391-19982410 None:intergenic 30.0%
! GAACAAAGGTGTAAACTTTA+TGG + chr4_3:19982863-19982882 Msa0633830:intron 30.0%
!!! TTATGGTCTTTTGAGACTTT+TGG + chr4_3:19982880-19982899 Msa0633830:intron 30.0%
!!! TATGGTCTTTTGAGACTTTT+GGG + chr4_3:19982881-19982900 Msa0633830:intron 30.0%
!! TTTTGAGGAATTTCAAGTGA+AGG + chr4_3:19983019-19983038 Msa0633830:CDS 30.0%
! TTTGAGGAATTTCAAGTGAA+GGG + chr4_3:19983020-19983039 Msa0633830:CDS 30.0%
AAGATGAAAATGGAGAACTA+GGG - chr4_3:19983193-19983212 None:intergenic 30.0%
GACAATGTTGAAGATGAAAA+TGG - chr4_3:19983203-19983222 None:intergenic 30.0%
GAGAATAGTGAAGAAAAATG+TGG + chr4_3:19983314-19983333 Msa0633830:CDS 30.0%
AATAGTGAAGAAAAATGTGG+TGG + chr4_3:19983317-19983336 Msa0633830:CDS 30.0%
!!! GTCACTTTTGAGGTTAATTA+TGG + chr4_3:19983379-19983398 Msa0633830:CDS 30.0%
! TTTTGTTCAATCCAGCATAA+GGG - chr4_3:19983418-19983437 None:intergenic 30.0%
! ATTTTGTTCAATCCAGCATA+AGG - chr4_3:19983419-19983438 None:intergenic 30.0%
TTGAACAAAATCTTGCAAAG+TGG + chr4_3:19983428-19983447 Msa0633830:CDS 30.0%
!!! CAAAATGTTGATTTTCATGG+TGG + chr4_3:19983464-19983483 Msa0633830:CDS 30.0%
! TCATGATGATGAGAAAACAA+TGG - chr4_3:19983662-19983681 None:intergenic 30.0%
! AATTGGTAAGCCTTTTCAAA+GGG + chr4_3:19983892-19983911 Msa0633830:CDS 30.0%
! TGAATGAAGCATTAACTGTA+AGG - chr4_3:19983939-19983958 None:intergenic 30.0%
CAGTTAATGCTTCATTCAAA+CGG + chr4_3:19983941-19983960 Msa0633830:CDS 30.0%
! ATTGATTTCGATATTGGGTT+TGG + chr4_3:19984118-19984137 Msa0633830:CDS 30.0%
GAGATTGTACTAAGAAGTAA+CGG + chr4_3:19984166-19984185 Msa0633830:CDS 30.0%
!!! CATACTGACTCAAATTTTCT+TGG - chr4_3:19984261-19984280 None:intergenic 30.0%
TTACTATCAAAGAAATGACC+CGG - chr4_3:19984358-19984377 None:intergenic 30.0%
! TTTGATAGTAATGAAGCCAT+TGG + chr4_3:19984367-19984386 Msa0633830:CDS 30.0%
TTACTTATGAAACTGGAAAC+AGG - chr4_3:19984403-19984422 None:intergenic 30.0%
!! TGATGGATTACTTATGAAAC+TGG - chr4_3:19984410-19984429 None:intergenic 30.0%
TTAAAAAATGAAGAGCCACA+GGG - chr4_3:19984438-19984457 None:intergenic 30.0%
TTTAAAAAATGAAGAGCCAC+AGG - chr4_3:19984439-19984458 None:intergenic 30.0%
ATGTACTCCAAAAGATTGAA+AGG + chr4_3:19984599-19984618 Msa0633830:CDS 30.0%
! TGTAATGCCTTTCAATCTTT+TGG - chr4_3:19984609-19984628 None:intergenic 30.0%
AAGGTTGAAATTTCTTAAGG+TGG + chr4_3:19984894-19984913 Msa0633830:three_prime_UTR 30.0%
AGTTAACAAACATTAGGGTA+TGG - chr4_3:19984982-19985001 None:intergenic 30.0%
! TGTTTGTTAACTAAATGGGT+TGG + chr4_3:19984990-19985009 Msa0633830:three_prime_UTR 30.0%
TATGATGTCTCTTTGAACAT+TGG + chr4_3:19985042-19985061 Msa0633830:three_prime_UTR 30.0%
AAAGAACAAGGACAAAGCTT+AGG - chr4_3:19982184-19982203 None:intergenic 35.0%
! AATTGCGTGCTGAGATATTT+TGG - chr4_3:19982345-19982364 None:intergenic 35.0%
ATCAAGATTCAAGACCCATT+TGG + chr4_3:19982745-19982764 Msa0633830:intron 35.0%
AGAGGGTAAGAAAAGAACAA+AGG + chr4_3:19982849-19982868 Msa0633830:intron 35.0%
! ATTTCAAGTGAAGGGAATGT+TGG + chr4_3:19983028-19983047 Msa0633830:CDS 35.0%
ATCAACCTCAGCAACAAAAT+TGG + chr4_3:19983105-19983124 Msa0633830:CDS 35.0%
! AATGACCAATTTTGTTGCTG+AGG - chr4_3:19983113-19983132 None:intergenic 35.0%
CTTGAATCAAGAACTGAAGT+GGG - chr4_3:19983164-19983183 None:intergenic 35.0%
TCTTGAATCAAGAACTGAAG+TGG - chr4_3:19983165-19983184 None:intergenic 35.0%
GAAGATGAAAATGGAGAACT+AGG - chr4_3:19983194-19983213 None:intergenic 35.0%
TCAACATTGTCATCTTCTCA+TGG + chr4_3:19983212-19983231 Msa0633830:CDS 35.0%
CATCATTTGAAAGATGAGCA+AGG - chr4_3:19983295-19983314 None:intergenic 35.0%
AACAAAATCTTGCAAAGTGG+TGG + chr4_3:19983431-19983450 Msa0633830:CDS 35.0%
!! GTTGATTTTCATGGTGGTTT+TGG + chr4_3:19983470-19983489 Msa0633830:CDS 35.0%
TGGATCATCAACAACAACAA+TGG + chr4_3:19983498-19983517 Msa0633830:CDS 35.0%
ACAACAACAATGGTTGAACA+TGG + chr4_3:19983508-19983527 Msa0633830:CDS 35.0%
TTGCAATTCTCAGGAATGAA+TGG - chr4_3:19983569-19983588 None:intergenic 35.0%
! CTTTGAAAAGGCTTACCAAT+TGG - chr4_3:19983893-19983912 None:intergenic 35.0%
! CAATTGGTAAGCCTTTTCAA+AGG + chr4_3:19983891-19983910 Msa0633830:CDS 35.0%
! TAGAAAGAAGCCCTTTGAAA+AGG - chr4_3:19983905-19983924 None:intergenic 35.0%
TTCAAACGGTAACAATCTCA+CGG + chr4_3:19983955-19983974 Msa0633830:CDS 35.0%
GGCTTGATTACAAGTAAAGT+TGG - chr4_3:19984059-19984078 None:intergenic 35.0%
! ATATTGGGTTTGGAGTACAA+TGG + chr4_3:19984128-19984147 Msa0633830:CDS 35.0%
ACAGCAGTAATCTTAAGTGA+AGG - chr4_3:19984196-19984215 None:intergenic 35.0%
TTGAAGAACATGAACGACAT+TGG - chr4_3:19984530-19984549 None:intergenic 35.0%
!! GTGAATTTGCTGAATGTGAA+GGG - chr4_3:19984727-19984746 None:intergenic 35.0%
CTTCACATTCAGCAAATTCA+CGG + chr4_3:19984726-19984745 Msa0633830:CDS 35.0%
AACTCATCTCAGTTTCAACT+TGG + chr4_3:19984845-19984864 Msa0633830:CDS 35.0%
! AACTTGGAGATGTTGATAAG+TGG + chr4_3:19984861-19984880 Msa0633830:exon 35.0%
GATAACAGAGAAACAGTGAA+AGG - chr4_3:19985086-19985105 None:intergenic 35.0%
AACCTTAGATCAAGAAAGAC+AGG - chr4_3:19985181-19985200 None:intergenic 35.0%
! TCTTGATCTAAGGTTGTGTT+TGG + chr4_3:19985186-19985205 Msa0633830:three_prime_UTR 35.0%
ATTTGGACATGTCAGAGACA+AGG - chr4_3:19982134-19982153 None:intergenic 40.0%
!!! AAATCTGTTTTCTGGGGTAG+TGG + chr4_3:19982814-19982833 Msa0633830:intron 40.0%
!! TTTGAGACTTTTGGGTCACA+TGG + chr4_3:19982889-19982908 Msa0633830:intron 40.0%
!! TTGAGACTTTTGGGTCACAT+GGG + chr4_3:19982890-19982909 Msa0633830:intron 40.0%
!! TTTTGGGTCACATGGGTTAT+TGG + chr4_3:19982897-19982916 Msa0633830:intron 40.0%
!!! CACATGGGTTATTGGTTTTG+TGG + chr4_3:19982905-19982924 Msa0633830:intron 40.0%
TTGTCATCTTCTCATGGTAG+TGG + chr4_3:19983218-19983237 Msa0633830:CDS 40.0%
!! TCTCATGGTAGTGGTAGTAA+TGG + chr4_3:19983227-19983246 Msa0633830:CDS 40.0%
! GGTAGTGGTAGTAATGGTAA+TGG + chr4_3:19983233-19983252 Msa0633830:CDS 40.0%
AGTGAAGAAAAATGTGGTGG+TGG + chr4_3:19983320-19983339 Msa0633830:CDS 40.0%
TGGTATGAGAATGGAGGATT+GGG + chr4_3:19983343-19983362 Msa0633830:CDS 40.0%
!!! GTCAAGATCAGTCACTTTTG+AGG + chr4_3:19983369-19983388 Msa0633830:CDS 40.0%
!!! TCATGGTGGTTTTGGAGTTT+TGG + chr4_3:19983478-19983497 Msa0633830:CDS 40.0%
GGTAACACATTGCAATTCTC+AGG - chr4_3:19983578-19983597 None:intergenic 40.0%
TTACCAACAACAACAGTCGT+CGG + chr4_3:19983694-19983713 Msa0633830:CDS 40.0%
! AAGGTGAGTTCTAGTACTAC+CGG + chr4_3:19983740-19983759 Msa0633830:CDS 40.0%
CAAATATACTCTGCTGAAGC+TGG - chr4_3:19983781-19983800 None:intergenic 40.0%
AAGGGCTTCTTTCTACATGA+AGG + chr4_3:19983910-19983929 Msa0633830:CDS 40.0%
CTGAAGAACAGGTGATATCT+CGG - chr4_3:19984032-19984051 None:intergenic 40.0%
AAGTTGGCAAACTGAAGAAC+AGG - chr4_3:19984043-19984062 None:intergenic 40.0%
TGAACTCAAACGACATGTTG+AGG - chr4_3:19984291-19984310 None:intergenic 40.0%
AACAGGAAAGTTCACACCAA+TGG - chr4_3:19984386-19984405 None:intergenic 40.0%
TCCAAAGTTACGACCACTTT+CGG - chr4_3:19984472-19984491 None:intergenic 40.0%
AGAACATGAACGACATTGGT+GGG - chr4_3:19984526-19984545 None:intergenic 40.0%
AAGAACATGAACGACATTGG+TGG - chr4_3:19984527-19984546 None:intergenic 40.0%
!!! CAATCTTTTGGAGTACATCG+AGG - chr4_3:19984597-19984616 None:intergenic 40.0%
!!! CGATTTTGTTGATGGTTGGT+TGG - chr4_3:19984633-19984652 None:intergenic 40.0%
!!! AGGACGATTTTGTTGATGGT+TGG - chr4_3:19984637-19984656 None:intergenic 40.0%
ACCATCAACAAAATCGTCCT+CGG + chr4_3:19984637-19984656 Msa0633830:CDS 40.0%
TGAAGAAACATGTTCCTCCA+TGG - chr4_3:19984691-19984710 None:intergenic 40.0%
AGGAACATGTTTCTTCAGTC+CGG + chr4_3:19984694-19984713 Msa0633830:CDS 40.0%
!! CGTGAATTTGCTGAATGTGA+AGG - chr4_3:19984728-19984747 None:intergenic 40.0%
! AAGCTGAGTGTTTGGTTCAA+AGG + chr4_3:19984755-19984774 Msa0633830:CDS 40.0%
! AGCTGAGTGTTTGGTTCAAA+GGG + chr4_3:19984756-19984775 Msa0633830:CDS 40.0%
CAGGACTAAACCATAAAGAG+AGG - chr4_3:19985162-19985181 None:intergenic 40.0%
GTCCTGTCTTTCTTGATCTA+AGG + chr4_3:19985176-19985195 Msa0633830:three_prime_UTR 40.0%
TAAACACTCGATCGAGTGTT+TGG + chr4_3:19985240-19985259 Msa0633830:three_prime_UTR 40.0%
CAATGAGGCAGGAAAGAACA+AGG - chr4_3:19982196-19982215 None:intergenic 45.0%
TGCTCAGATATCAATGAGGC+AGG - chr4_3:19982207-19982226 None:intergenic 45.0%
!!! TTCTGGGGTAGTGGATTTTG+TGG + chr4_3:19982823-19982842 Msa0633830:intron 45.0%
!!! TCTGGGGTAGTGGATTTTGT+GGG + chr4_3:19982824-19982843 Msa0633830:intron 45.0%
!!! TAGTGGATTTTGTGGGGAAG+AGG + chr4_3:19982831-19982850 Msa0633830:intron 45.0%
!! AGTGGATTTTGTGGGGAAGA+GGG + chr4_3:19982832-19982851 Msa0633830:intron 45.0%
! GGCTTTGCCCTTAACTTTTG+AGG + chr4_3:19983004-19983023 Msa0633830:CDS 45.0%
!! GAAGAAAAATGTGGTGGTGG+TGG + chr4_3:19983323-19983342 Msa0633830:CDS 45.0%
GTGGTATGAGAATGGAGGAT+TGG + chr4_3:19983342-19983361 Msa0633830:CDS 45.0%
ATGAGAATGGAGGATTGGGA+AGG + chr4_3:19983347-19983366 Msa0633830:CDS 45.0%
GATGTTGAAGACCCTTATGC+TGG + chr4_3:19983404-19983423 Msa0633830:CDS 45.0%
ATCTTGCAAAGTGGTGGTTG+TGG + chr4_3:19983437-19983456 Msa0633830:CDS 45.0%
ATGGTTGAACATGGACGCTT+CGG + chr4_3:19983517-19983536 Msa0633830:CDS 45.0%
TTGCAATGTGTTACCGCTCT+TGG + chr4_3:19983583-19983602 Msa0633830:CDS 45.0%
TCTTGACCAGAATCCAAGAG+CGG - chr4_3:19983599-19983618 None:intergenic 45.0%
!! CTGGTCAAGAAGTGTTTGCT+AGG + chr4_3:19983609-19983628 Msa0633830:CDS 45.0%
TCCAATTGCGAAGCAGCAAA+AGG + chr4_3:19983721-19983740 Msa0633830:CDS 45.0%
!! ACCTTTTGCTGCTTCGCAAT+TGG - chr4_3:19983725-19983744 None:intergenic 45.0%
GCTGATAGAAGCTGGTAATC+CGG + chr4_3:19983823-19983842 Msa0633830:CDS 45.0%
AATATCCCTTGCGCTTGAAC+CGG - chr4_3:19983845-19983864 None:intergenic 45.0%
AATCACCAGCTCTCACCAAT+TGG + chr4_3:19983875-19983894 Msa0633830:CDS 45.0%
AATTCGATCGAACCTCTCCA+AGG - chr4_3:19984092-19984111 None:intergenic 45.0%
TCAATCTCGTTGCAAGACGA+GGG - chr4_3:19984226-19984245 None:intergenic 45.0%
TCAAAGAAATGACCCGGTAG+AGG - chr4_3:19984352-19984371 None:intergenic 45.0%
TAATCCATCATGCTTCCCTG+TGG + chr4_3:19984420-19984439 Msa0633830:CDS 45.0%
TGGGAAGAGGAACATCCATT+CGG - chr4_3:19984507-19984526 None:intergenic 45.0%
! ACCGAGGACGATTTTGTTGA+TGG - chr4_3:19984641-19984660 None:intergenic 45.0%
! GTAATTTGTCACGCTGGTTG+TGG - chr4_3:19984666-19984685 None:intergenic 45.0%
! ATGGAGGTAATTTGTCACGC+TGG - chr4_3:19984672-19984691 None:intergenic 45.0%
AGCGTGACAAATTACCTCCA+TGG + chr4_3:19984671-19984690 Msa0633830:CDS 45.0%
GTGACAAATTACCTCCATGG+AGG + chr4_3:19984674-19984693 Msa0633830:CDS 45.0%
AGAAACATGTTCCTCCATGG+AGG - chr4_3:19984688-19984707 None:intergenic 45.0%
GAATGTGAAGGGAGAGAAAC+CGG - chr4_3:19984716-19984735 None:intergenic 45.0%
ACCTGTGAGAGGATTTCAAG+TGG + chr4_3:19984780-19984799 Msa0633830:CDS 45.0%
TCCACTTGAAATCCTCTCAC+AGG - chr4_3:19984784-19984803 None:intergenic 45.0%
!!! TGTTTTTTAAATTTTTTAAT+GGG - chr4_3:19982320-19982339 None:intergenic 5.0%
!!! TTGTTTTTTAAATTTTTTAA+TGG - chr4_3:19982321-19982340 None:intergenic 5.0%
!! TAAAATAAAAATAAAAAAAC+AGG - chr4_3:19982576-19982595 None:intergenic 5.0%
AGATGGATGCGTGCATACGT+AGG - chr4_3:19982374-19982393 None:intergenic 50.0%
!!! CTGGGGTAGTGGATTTTGTG+GGG + chr4_3:19982825-19982844 Msa0633830:intron 50.0%
!! TGGTGGTGGTGGTATGAGAA+TGG + chr4_3:19983334-19983353 Msa0633830:CDS 50.0%
!! TGGTGGTGGTATGAGAATGG+AGG + chr4_3:19983337-19983356 Msa0633830:CDS 50.0%
ATGGACGCTTCGGTTCAACA+AGG + chr4_3:19983527-19983546 Msa0633830:CDS 50.0%
TGGACGCTTCGGTTCAACAA+GGG + chr4_3:19983528-19983547 Msa0633830:CDS 50.0%
GTGTTACCGCTCTTGGATTC+TGG + chr4_3:19983590-19983609 Msa0633830:CDS 50.0%
CAACCGACGACTGTTGTTGT+TGG - chr4_3:19983700-19983719 None:intergenic 50.0%
CTGGATGCTTGCATCATCTC+CGG - chr4_3:19983762-19983781 None:intergenic 50.0%
ACTGCTGAGCTGATAGAAGC+TGG + chr4_3:19983815-19983834 Msa0633830:CDS 50.0%
GTAATCCGGTTCAAGCGCAA+GGG + chr4_3:19983837-19983856 Msa0633830:CDS 50.0%
GGTTCAAGCGCAAGGGATAT+TGG + chr4_3:19983844-19983863 Msa0633830:CDS 50.0%
GCTTACCAATTGGTGAGAGC+TGG - chr4_3:19983883-19983902 None:intergenic 50.0%
TCAAGCCCTCATTGAAGCCT+TGG + chr4_3:19984072-19984091 Msa0633830:CDS 50.0%
CCTCTCCAAGGCTTCAATGA+GGG - chr4_3:19984080-19984099 None:intergenic 50.0%
ACCTCTCCAAGGCTTCAATG+AGG - chr4_3:19984081-19984100 None:intergenic 50.0%
CTCAATCTCGTTGCAAGACG+AGG - chr4_3:19984227-19984246 None:intergenic 50.0%
CTTGCCATCATGTCCTCTAC+CGG + chr4_3:19984336-19984355 Msa0633830:CDS 50.0%
TTGCCATCATGTCCTCTACC+GGG + chr4_3:19984337-19984356 Msa0633830:CDS 50.0%
AAAGCAGCTAAGGCCGAAAG+TGG + chr4_3:19984456-19984475 Msa0633830:CDS 50.0%
GCCGAAAGTGGTCGTAACTT+TGG + chr4_3:19984468-19984487 Msa0633830:CDS 50.0%
GGACAAAAACTGCGACCGAA+TGG + chr4_3:19984489-19984508 Msa0633830:CDS 50.0%
TGAACGACATTGGTGGGAAG+AGG - chr4_3:19984520-19984539 None:intergenic 50.0%
GGAAGCTCAAGCTGAGTGTT+TGG + chr4_3:19984747-19984766 Msa0633830:CDS 50.0%
CAGCATAGCACAAGAGAGGA+AGG - chr4_3:19984814-19984833 None:intergenic 50.0%
CTTCCTCTCTTGTGCTATGC+TGG + chr4_3:19984812-19984831 Msa0633830:CDS 50.0%
TGCTAGGAGACATCAGCAGC+AGG + chr4_3:19983625-19983644 Msa0633830:CDS 55.0%
GGTAATCCGGTTCAAGCGCA+AGG + chr4_3:19983836-19983855 Msa0633830:CDS 55.0%
AAGCGCAAGGGATATTGGCG+CGG + chr4_3:19983849-19983868 Msa0633830:CDS 55.0%
CCCTCATTGAAGCCTTGGAG+AGG + chr4_3:19984077-19984096 Msa0633830:CDS 55.0%
TGACCCGGTAGAGGACATGA+TGG - chr4_3:19984343-19984362 None:intergenic 55.0%
! AGAGCCACAGGGAAGCATGA+TGG - chr4_3:19984427-19984446 None:intergenic 55.0%
!! GTTCAAAGGGCACCTGTGAG+AGG + chr4_3:19984769-19984788 Msa0633830:CDS 55.0%
CTGCCAGCATAGCACAAGAG+AGG - chr4_3:19984818-19984837 None:intergenic 55.0%
CTCTTGTGCTATGCTGGCAG+AGG + chr4_3:19984818-19984837 Msa0633830:CDS 55.0%
! CACGCTGGTTGTGGTGACCG+AGG - chr4_3:19984657-19984676 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 19982099 19985316 19982099 ID=Msa0633830;Name=Msa0633830
chr4_3 mRNA 19982099 19985316 19982099 ID=Msa0633830-mRNA-1;Parent=Msa0633830;Name=Msa0633830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=456|1|1|1|0|0.5|2|440|625
chr4_3 exon 19982099 19982554 19982099 ID=Msa0633830-mRNA-1:exon:5356;Parent=Msa0633830-mRNA-1
chr4_3 exon 19982999 19985316 19982999 ID=Msa0633830-mRNA-1:exon:5357;Parent=Msa0633830-mRNA-1
chr4_3 five_prime_UTR 19982099 19982554 19982099 ID=Msa0633830-mRNA-1:five_prime_utr;Parent=Msa0633830-mRNA-1
chr4_3 CDS 19982999 19984876 19982999 ID=Msa0633830-mRNA-1:cds;Parent=Msa0633830-mRNA-1
chr4_3 three_prime_UTR 19984877 19985316 19984877 ID=Msa0633830-mRNA-1:three_prime_utr;Parent=Msa0633830-mRNA-1
Gene Sequence

>Msa0633830

ATGAAGGCTTTGCCCTTAACTTTTGAGGAATTTCAAGTGAAGGGAATGTTGGATAATTTCTCTTCAACTTCAAATTCAGTTTCAGATTCATTTTCATTGCTTTTGAATCAACCTCAGCAACAAAATTGGTCATTAGATAAAGAAGATTATTGCTATGTGGAGCCCACTTCAGTTCTTGATTCAAGAAGAAGCCCTAGTTCTCCATTTTCATCTTCAACATTGTCATCTTCTCATGGTAGTGGTAGTAATGGTAATGGTTCAAATAGCAATACTTCAATGGTAGTTTTTGAGAACCTTGCTCATCTTTCAAATGATGAGAATAGTGAAGAAAAATGTGGTGGTGGTGGTATGAGAATGGAGGATTGGGAAGGTCAAGATCAGTCACTTTTGAGGTTAATTATGGGAGATGTTGAAGACCCTTATGCTGGATTGAACAAAATCTTGCAAAGTGGTGGTTGTGGTTCTCAAAATGTTGATTTTCATGGTGGTTTTGGAGTTTTGGATCATCAACAACAACAATGGTTGAACATGGACGCTTCGGTTCAACAAGGGAATTACAATGTTTTTCCATTCATTCCTGAGAATTGCAATGTGTTACCGCTCTTGGATTCTGGTCAAGAAGTGTTTGCTAGGAGACATCAGCAGCAGGAAACACAGCTTCCATTGTTTTCTCATCATCATGATCTGCAGCAGCATTACCAACAACAACAGTCGTCGGTTGTTCCAATTGCGAAGCAGCAAAAGGTGAGTTCTAGTACTACCGGAGATGATGCAAGCATCCAGCTTCAGCAGAGTATATTTGATCAGCTATTCAAAACTGCTGAGCTGATAGAAGCTGGTAATCCGGTTCAAGCGCAAGGGATATTGGCGCGGCTCAATCACCAGCTCTCACCAATTGGTAAGCCTTTTCAAAGGGCTTCTTTCTACATGAAGGAAGCCTTACAGTTAATGCTTCATTCAAACGGTAACAATCTCACGGCTTTTTCGCCGATTAGTTTCATTTTCAAAATCGGAGCTTATAAGTCTTTTTCCGAGATATCACCTGTTCTTCAGTTTGCCAACTTTACTTGTAATCAAGCCCTCATTGAAGCCTTGGAGAGGTTCGATCGAATTCATGTTATTGATTTCGATATTGGGTTTGGAGTACAATGGTCGTCTTTTATGCAAGAGATTGTACTAAGAAGTAACGGTAAGCCTTCACTTAAGATTACTGCTGTTGTATCTCCCTCGTCTTGCAACGAGATTGAGCTCAATTTCACCCAAGAAAATTTGAGTCAGTATGCAAAAGACCTCAACATGTCGTTTGAGTTCAACGTTTTGAACATTGAATCGTTGAACTTGCCATCATGTCCTCTACCGGGTCATTTCTTTGATAGTAATGAAGCCATTGGTGTGAACTTTCCTGTTTCCAGTTTCATAAGTAATCCATCATGCTTCCCTGTGGCTCTTCATTTTTTAAAGCAGCTAAGGCCGAAAGTGGTCGTAACTTTGGACAAAAACTGCGACCGAATGGATGTTCCTCTTCCCACCAATGTCGTTCATGTTCTTCAATGTTACTCAGCTTTGCTTGAATCACTTGATGCTGTGAACGTGAACCTCGATGTACTCCAAAAGATTGAAAGGCATTACATCCAACCAACCATCAACAAAATCGTCCTCGGTCACCACAACCAGCGTGACAAATTACCTCCATGGAGGAACATGTTTCTTCAGTCCGGTTTCTCTCCCTTCACATTCAGCAAATTCACGGAAGCTCAAGCTGAGTGTTTGGTTCAAAGGGCACCTGTGAGAGGATTTCAAGTGGAAAGAAAGCCTTCCTCTCTTGTGCTATGCTGGCAGAGGAAAGAACTCATCTCAGTTTCAACTTGGAGATGTTGA
Protein sequence

>Msa0633830

MKALPLTFEEFQVKGMLDNFSSTSNSVSDSFSLLLNQPQQQNWSLDKEDYCYVEPTSVLDSRRSPSSPFSSSTLSSSHGSGSNGNGSNSNTSMVVFENLAHLSNDENSEEKCGGGGMRMEDWEGQDQSLLRLIMGDVEDPYAGLNKILQSGGCGSQNVDFHGGFGVLDHQQQQWLNMDASVQQGNYNVFPFIPENCNVLPLLDSGQEVFARRHQQQETQLPLFSHHHDLQQHYQQQQSSVVPIAKQQKVSSSTTGDDASIQLQQSIFDQLFKTAELIEAGNPVQAQGILARLNHQLSPIGKPFQRASFYMKEALQLMLHSNGNNLTAFSPISFIFKIGAYKSFSEISPVLQFANFTCNQALIEALERFDRIHVIDFDIGFGVQWSSFMQEIVLRSNGKPSLKITAVVSPSSCNEIELNFTQENLSQYAKDLNMSFEFNVLNIESLNLPSCPLPGHFFDSNEAIGVNFPVSSFISNPSCFPVALHFLKQLRPKVVVTLDKNCDRMDVPLPTNVVHVLQCYSALLESLDAVNVNLDVLQKIERHYIQPTINKIVLGHHNQRDKLPPWRNMFLQSGFSPFTFSKFTEAQAECLVQRAPVRGFQVERKPSSLVLCWQRKELISVSTWRC*