AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0633950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0633950 MtrunA17_Chr2g0296781 75.364 686 167 2 120 805 1 684 0.0 1077
Msa0633950 MtrunA17_Chr6g0477261 77.526 574 128 1 232 805 1 573 0.0 921
Msa0633950 MtrunA17_Chr1g0196541 65.312 689 232 2 120 808 1 682 0.0 917
Msa0633950 MtrunA17_Chr5g0441581 48.226 620 304 6 110 723 21 629 0.0 583
Msa0633950 MtrunA17_Chr2g0291581 37.797 590 347 9 94 677 47 622 4.21e-132 409
Msa0633950 MtrunA17_Chr1g0207551 36.907 569 348 5 113 678 31 591 6.97e-126 397
Msa0633950 MtrunA17_Chr5g0448761 37.308 587 343 11 110 681 54 630 2.79e-123 389
Msa0633950 MtrunA17_Chr1g0202341 37.805 574 333 11 115 677 50 610 1.76e-122 384
Msa0633950 MtrunA17_Chr2g0329031 36.207 580 345 10 108 681 6 566 2.38e-121 383
Msa0633950 MtrunA17_Chr3g0080351 35.775 587 328 9 110 678 70 625 3.57e-117 375
Msa0633950 MtrunA17_Chr2g0291591 34.535 527 339 4 85 608 33 556 1.82e-106 338
Msa0633950 MtrunA17_Chr5g0440451 32.353 612 367 14 115 702 42 630 2.20e-93 313
Msa0633950 MtrunA17_Chr5g0448511 36.700 406 245 5 274 676 1 397 1.04e-86 285
Msa0633950 MtrunA17_Chr2g0287651 28.479 618 425 7 120 730 79 686 1.88e-86 293
Msa0633950 MtrunA17_Chr3g0118611 37.530 413 244 5 286 697 118 517 1.06e-84 286
Msa0633950 MtrunA17_Chr2g0324011 29.879 579 374 10 113 676 7 568 9.88e-82 273
Msa0633950 MtrunA17_Chr8g0363841 30.703 583 371 12 112 676 10 577 8.20e-80 270
Msa0633950 MtrunA17_Chr2g0287061 31.634 569 329 13 115 674 182 699 1.44e-78 270
Msa0633950 MtrunA17_Chr2g0276841 28.869 672 409 19 106 742 60 697 2.90e-75 259
Msa0633950 MtrunA17_Chr6g0476441 68.947 190 58 1 616 805 1 189 6.95e-75 245
Msa0633950 MtrunA17_Chr1g0198761 28.693 704 424 24 119 783 50 714 4.37e-72 251
Msa0633950 MtrunA17_Chr6g0458391 28.399 581 379 12 105 676 213 765 1.07e-70 248
Msa0633950 MtrunA17_Chr1g0203051 27.258 609 399 13 76 657 25 616 2.23e-68 238
Msa0633950 MtrunA17_Chr1g0203721 29.145 573 378 10 118 676 65 623 3.64e-68 243
Msa0633950 MtrunA17_Chr3g0082121 30.298 604 369 18 95 676 17 590 1.57e-65 232
Msa0633950 MtrunA17_Chr2g0294841 26.783 575 388 11 116 672 45 604 5.62e-61 220
Msa0633950 MtrunA17_Chr2g0290391 26.030 607 403 16 92 674 27 611 1.44e-51 192
Msa0633950 MtrunA17_Chr5g0431001 33.019 318 202 5 209 520 168 480 2.84e-51 187
Msa0633950 MtrunA17_Chr1g0164591 33.016 315 187 7 198 504 10 308 5.50e-47 170
Msa0633950 MtrunA17_Chr7g0258861 33.101 287 186 3 109 389 3 289 9.39e-47 169
Msa0633950 MtrunA17_Chr6g0476431 59.302 86 35 0 120 205 1 86 2.67e-30 114
Msa0633950 MtrunA17_Chr6g0451441 30.851 188 122 4 96 278 63 247 4.71e-20 94.4
Msa0633950 MtrunA17_Chr2g0308841 25.108 231 158 3 448 673 4 224 1.90e-19 89.4
Msa0633950 MtrunA17_Chr1g0172041 33.636 110 72 1 588 697 1 109 5.71e-14 68.9
Msa0633950 MtrunA17_Chr3g0077731 58.442 77 31 1 729 805 1 76 2.42e-13 67.4
Msa0633950 MtrunA17_Chr2g0329361 30.400 125 71 3 115 228 60 179 3.49e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0633950 AT4G15090.1 34.191 661 417 10 98 751 33 682 3.09e-128 404
Msa0633950 AT4G15090.5 34.191 661 417 10 98 751 33 682 3.09e-128 404
Msa0633950 AT4G15090.3 34.191 661 417 10 98 751 33 682 3.09e-128 404
Msa0633950 AT4G15090.2 34.191 661 417 10 98 751 33 682 3.09e-128 404
Msa0633950 AT4G15090.4 34.191 661 417 10 98 751 33 682 3.09e-128 404
Msa0633950 AT4G38180.1 35.052 582 363 8 103 676 60 634 1.28e-117 375
Msa0633950 AT1G52520.1 34.522 617 372 13 69 677 47 639 7.18e-117 370
Msa0633950 AT3G22170.2 35.993 589 329 10 109 679 63 621 4.59e-116 372
Msa0633950 AT3G22170.1 35.993 589 329 10 109 679 63 621 4.59e-116 372
Msa0633950 AT1G76320.3 36.111 576 339 10 120 684 1 558 5.11e-113 359
Msa0633950 AT1G76320.2 36.111 576 339 10 120 684 1 558 1.34e-112 360
Msa0633950 AT1G76320.1 36.111 576 339 10 120 684 1 558 1.79e-112 360
Msa0633950 AT1G76320.4 36.111 576 339 10 120 684 1 558 1.79e-112 360
Msa0633950 AT1G80010.1 34.609 601 357 16 103 680 78 665 9.40e-105 339
Msa0633950 AT4G19990.3 32.660 594 316 10 119 681 24 564 6.53e-93 308
Msa0633950 AT4G19990.4 32.660 594 316 10 119 681 24 564 6.53e-93 308
Msa0633950 AT4G19990.5 32.660 594 316 10 119 681 24 564 7.38e-93 308
Msa0633950 AT4G19990.6 32.660 594 316 10 119 681 24 564 8.83e-93 308
Msa0633950 AT4G19990.2 32.660 594 316 10 119 681 24 564 8.83e-93 308
Msa0633950 AT4G19990.7 32.660 594 316 10 119 681 38 578 9.82e-93 308
Msa0633950 AT2G27110.2 32.318 591 364 8 91 676 20 579 9.33e-92 307
Msa0633950 AT2G27110.1 32.318 591 364 8 91 676 20 579 9.33e-92 307
Msa0633950 AT4G19990.1 33.952 539 300 9 146 681 22 507 4.70e-91 301
Msa0633950 AT2G32250.5 32.918 562 326 8 115 673 37 550 1.19e-90 302
Msa0633950 AT2G32250.6 32.918 562 326 8 115 673 37 550 2.44e-90 303
Msa0633950 AT2G32250.1 32.918 562 326 8 115 673 37 550 2.44e-90 303
Msa0633950 AT2G32250.3 32.918 562 326 8 115 673 37 550 2.44e-90 303
Msa0633950 AT2G32250.2 32.918 562 326 8 115 673 37 550 2.80e-90 302
Msa0633950 AT2G32250.4 32.918 562 326 8 115 673 37 550 2.80e-90 302
Msa0633950 AT2G32250.7 32.855 557 323 8 120 673 1 509 1.03e-89 300
Msa0633950 AT2G32250.8 32.855 557 323 8 120 673 1 509 1.24e-89 300
Msa0633950 AT2G27110.3 34.554 437 269 5 241 676 14 434 2.25e-81 276
Msa0633950 AT3G06250.2 30.382 576 341 12 106 674 180 702 7.74e-79 270
Msa0633950 AT3G06250.1 30.382 576 341 12 106 674 180 702 7.74e-79 270
Msa0633950 AT3G06250.3 30.382 576 341 12 106 674 180 702 7.74e-79 270
Msa0633950 AT4G38170.1 34.937 395 245 5 288 680 19 403 4.00e-76 257
Msa0633950 AT5G18960.2 31.217 567 333 17 115 674 210 726 8.24e-76 263
Msa0633950 AT5G18960.1 31.217 567 333 17 115 674 210 726 8.24e-76 263
Msa0633950 AT1G10240.2 28.595 619 376 19 88 673 19 604 1.08e-67 238
Msa0633950 AT1G10240.1 28.595 619 376 19 88 673 19 604 1.08e-67 238
Msa0633950 AT5G28530.3 25.463 648 417 19 116 728 55 671 2.30e-51 191
Msa0633950 AT5G28530.2 25.463 648 417 19 116 728 55 671 2.30e-51 191
Msa0633950 AT5G28530.1 25.460 652 416 20 116 728 55 675 1.63e-50 189
Msa0633950 AT5G28530.5 26.649 379 247 12 359 728 252 608 1.16e-27 119
Msa0633950 AT5G28530.4 26.632 383 246 13 359 728 252 612 8.64e-27 117

Find 110 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGTTTATTTGAGTTTA+AGG 0.126982 4_3:+20404439 Msa0633950:CDS
ATTGCTTGTATAAGTCATTT+TGG 0.199496 4_3:+20404200 Msa0633950:CDS
CTTGAACTGGATGCTAAAAT+AGG 0.226174 4_3:-20402641 None:intergenic
AAGAATTATGCAAGATGTTT+AGG 0.244212 4_3:+20402931 Msa0633950:CDS
TATGCAAGATGTTTAGGTTT+TGG 0.256539 4_3:+20402937 Msa0633950:CDS
TCCATCCTGGTCAGTAATTA+TGG 0.258712 4_3:-20403727 None:intergenic
TTTGAGCATAAGTGATTTCA+TGG 0.262311 4_3:+20403899 Msa0633950:CDS
TGTATGTGAGAGACACATTT+TGG 0.270477 4_3:+20404012 Msa0633950:CDS
CACCATCGATGGCGAGCTTT+CGG 0.279320 4_3:-20403780 None:intergenic
TGGAACAATTACTATTTCTA+AGG 0.295449 4_3:+20403125 Msa0633950:CDS
GGAAATCAAATTTCAATTGA+TGG 0.300295 4_3:-20402619 None:intergenic
TAAAATTGAAAAGGAAAATA+TGG 0.301034 4_3:+20404151 Msa0633950:CDS
TATACAAGCAATAATTCTAT+TGG 0.306437 4_3:-20404189 None:intergenic
TAGTATCCTCCTTCGACAAT+AGG 0.312589 4_3:-20403644 None:intergenic
AGGGCTGCTTATGAGTATTT+TGG 0.315150 4_3:+20403513 Msa0633950:CDS
TGAAGAAGCAAATCCGTAAA+AGG 0.325895 4_3:-20404645 None:intergenic
TGCTGTTTATTTGAGTTTAA+GGG 0.334665 4_3:+20404440 Msa0633950:CDS
CTAAAATATCTTGATTTGCT+TGG 0.342688 4_3:-20403180 None:intergenic
ATATAGACCCTAGTATAAAA+AGG 0.351811 4_3:+20403214 Msa0633950:CDS
ACGCTGCAACTCTTCATTTC+CGG 0.353530 4_3:-20404603 None:intergenic
GAACAAAATCAGAACATATA+AGG 0.353968 4_3:-20402493 None:intergenic
TTTCTTGGTTGTTCACTACT+TGG 0.357896 4_3:+20403673 Msa0633950:CDS
CTTACTATTGTCTCTTGAAC+TGG 0.373416 4_3:-20402654 None:intergenic
TCATGCTTTCTTTGAGAGAA+TGG 0.375982 4_3:+20404304 Msa0633950:CDS
ACCATCGATGGCGAGCTTTC+GGG 0.381382 4_3:-20403779 None:intergenic
TTGTGTTACAGAAAGTAAAA+AGG 0.383201 4_3:+20404346 Msa0633950:CDS
CATGGTCAGTCAGTTCTGTT+AGG 0.383640 4_3:+20403615 Msa0633950:CDS
CTTGTGGCATATAATGAAAA+AGG 0.386165 4_3:+20403803 Msa0633950:CDS
TAGAATAGAGAACTTAAAAC+TGG 0.390920 4_3:+20404706 Msa0633950:CDS
AGTATCCTCCTTCGACAATA+GGG 0.392582 4_3:-20403643 None:intergenic
TTGAACTGGATGCTAAAATA+GGG 0.396461 4_3:-20402640 None:intergenic
AATCAGAACATATAAGGTTA+TGG 0.408232 4_3:-20402487 None:intergenic
GGATATGATAACCTTACATT+CGG 0.409109 4_3:+20403309 Msa0633950:CDS
CCTTTACTTCGAGTCGCTAC+AGG 0.409218 4_3:-20404809 None:intergenic
AAGGGCGTCCTCCTTCAAAA+AGG 0.415773 4_3:+20404828 Msa0633950:CDS
TTAAATTTCATGCCAACCTT+AGG 0.424975 4_3:-20402880 None:intergenic
GTTTGATGAGCGTCATCGTT+GGG 0.431278 4_3:+20403983 Msa0633950:CDS
AAGTGAGGCGGCTTCGACTT+GGG 0.432744 4_3:+20403361 Msa0633950:CDS
ATATTTATTTGTTAAGTAAG+TGG 0.436838 4_3:-20403556 None:intergenic
TTAATATCCTTCCTCCATCT+TGG 0.438245 4_3:-20404539 None:intergenic
CTTGTTATATATTGCCAAGA+TGG 0.439590 4_3:+20404525 Msa0633950:CDS
AAGAAGCTTGCTGGAAAGAA+AGG 0.445180 4_3:+20404881 Msa0633950:CDS
CATTCTTCATTGCTCCATCC+TGG 0.449843 4_3:-20403740 None:intergenic
CATGATAATGAATGGTTGAA+AGG 0.452805 4_3:+20403957 Msa0633950:CDS
GATATGATAACCTTACATTC+GGG 0.457280 4_3:+20403310 Msa0633950:CDS
ATTTGAATCTCAATTTCAAA+AGG 0.459389 4_3:+20404223 Msa0633950:CDS
CAAATTTCAATTGATGGTTG+AGG 0.460215 4_3:-20402613 None:intergenic
ACTCCAACAAAAGGAGCAAA+AGG 0.476605 4_3:-20403585 None:intergenic
CAAAGAGTTTCAAGTAGAAG+TGG 0.481571 4_3:+20404265 Msa0633950:CDS
AGATATACACTTATTAAAAG+TGG 0.483850 4_3:+20404566 Msa0633950:CDS
TTTGGGCAGATGCGAGATGT+AGG 0.485083 4_3:+20403493 Msa0633950:CDS
TACTTACATTAATCCCAGCT+TGG 0.488151 4_3:-20403254 None:intergenic
CCTGTAGCGACTCGAAGTAA+AGG 0.488591 4_3:+20404809 Msa0633950:CDS
TAGCATGAAAACACACTGTA+AGG 0.490277 4_3:+20403074 Msa0633950:CDS
AGTTAAAGACATAATGTTCA+AGG 0.493487 4_3:+20404379 Msa0633950:CDS
CTGTAGCGACTCGAAGTAAA+GGG 0.498287 4_3:+20404810 Msa0633950:CDS
ATTGAAAGATAAAATTGAAA+AGG 0.501824 4_3:+20404142 Msa0633950:CDS
TCCCGAAAGCTCGCCATCGA+TGG 0.503126 4_3:+20403778 Msa0633950:CDS
TTTGTTGGAGTAAATCATCA+TGG 0.506547 4_3:+20403597 Msa0633950:CDS
AGGCGGCTTCGACTTGGGAC+AGG 0.507987 4_3:+20403366 Msa0633950:CDS
TTGGGCAGATGCGAGATGTA+GGG 0.512318 4_3:+20403494 Msa0633950:CDS
TGATGATTTACTCCAACAAA+AGG 0.515106 4_3:-20403594 None:intergenic
TGTTTGATGAGCGTCATCGT+TGG 0.518510 4_3:+20403982 Msa0633950:CDS
AAAGTGAGGCGGCTTCGACT+TGG 0.518819 4_3:+20403360 Msa0633950:CDS
GACGACAACGAGTTTGAAAA+CGG 0.527121 4_3:-20402551 None:intergenic
CGGCGGTGAATGTTGGTAAC+CGG 0.527849 4_3:-20402531 None:intergenic
TTGGAGGTTAATGACCAAGC+TGG 0.528235 4_3:+20403240 Msa0633950:CDS
ATGATAATGAATGGTTGAAA+GGG 0.531139 4_3:+20403958 Msa0633950:CDS
AAATGATTTATCAAAAGTAA+TGG 0.531224 4_3:+20404682 Msa0633950:CDS
AACATATAAGGTTATGGAGA+AGG 0.534401 4_3:-20402481 None:intergenic
TGGAGGTTAATGACCAAGCT+GGG 0.539674 4_3:+20403241 Msa0633950:CDS
CTCGCCATCGATGGTGCTTG+TGG 0.540244 4_3:+20403787 Msa0633950:CDS
TACTCACATAGCTTCTTGCA+CGG 0.540398 4_3:-20403023 None:intergenic
ATGAACTTCATGATAATGAA+TGG 0.543870 4_3:+20403949 Msa0633950:CDS
AAATGAAGATGATGAACCTA+AGG 0.544931 4_3:+20402864 Msa0633950:CDS
TTGAAAACGGCGGTGAATGT+TGG 0.547831 4_3:-20402538 None:intergenic
AAGTGATTTCATGGAGAAGT+GGG 0.549527 4_3:+20403908 Msa0633950:CDS
GAAGATGATGAACCTAAGGT+TGG 0.554642 4_3:+20402868 Msa0633950:CDS
AAATCCGTAAAAGGCATCGC+AGG 0.562477 4_3:-20404636 None:intergenic
TAAGTGATTTCATGGAGAAG+TGG 0.562788 4_3:+20403907 Msa0633950:CDS
GTTCAACAACACAAACTCAC+CGG 0.564921 4_3:+20402512 Msa0633950:CDS
ATGGCGAGCTTTCGGGAAGA+CGG 0.568527 4_3:-20403772 None:intergenic
AATGTATGTGTGTCTTCAGA+TGG 0.571681 4_3:+20403105 Msa0633950:CDS
TGGTCAGTAATTATGGCTTG+TGG 0.579259 4_3:-20403720 None:intergenic
TGCTTTCTTTGAGAGAATGG+AGG 0.581817 4_3:+20404307 Msa0633950:CDS
GTTATATATTGCCAAGATGG+AGG 0.585753 4_3:+20404528 Msa0633950:CDS
AGGTTGCGCCCTATTGTCGA+AGG 0.587751 4_3:+20403635 Msa0633950:CDS
TGTTAAGTAAGTGGTGTCGA+AGG 0.588670 4_3:-20403547 None:intergenic
GAAACTACATTGACAAAGTG+AGG 0.593331 4_3:+20403346 Msa0633950:CDS
TATATTGCCAAGATGGAGGA+AGG 0.599926 4_3:+20404532 Msa0633950:CDS
CACTTATCAAAGTCAAGAAG+AGG 0.608354 4_3:+20404928 Msa0633950:CDS
ACTTGGTTAGAATGTATGCG+TGG 0.608573 4_3:+20403690 Msa0633950:CDS
TCTTGGCAATATATAACAAG+AGG 0.613918 4_3:-20404522 None:intergenic
CATGAAATTTAATTCTACAG+AGG 0.616241 4_3:+20402891 Msa0633950:CDS
CTTGGTTAGAATGTATGCGT+GGG 0.625544 4_3:+20403691 Msa0633950:CDS
ACAAGCCATAATTACTGACC+AGG 0.626330 4_3:+20403722 Msa0633950:CDS
AGCAATGAAGAATGCAGTTG+AGG 0.631682 4_3:+20403749 Msa0633950:CDS
AATGTGTCTCTCACATACAC+AGG 0.631924 4_3:-20404008 None:intergenic
GCCATAATTACTGACCAGGA+TGG 0.636449 4_3:+20403726 Msa0633950:CDS
TCTATGGTTGTTGAAGCAAG+TGG 0.641961 4_3:+20403288 Msa0633950:CDS
CTTGGCAATATATAACAAGA+GGG 0.647143 4_3:-20404521 None:intergenic
TCACATAAATGAAAATTGTG+AGG 0.656952 4_3:+20402828 Msa0633950:CDS
TATGCCACAAGCACCATCGA+TGG 0.658002 4_3:-20403791 None:intergenic
TTGCGCCCTATTGTCGAAGG+AGG 0.658239 4_3:+20403638 Msa0633950:CDS
ATATATGGAAGAGACAATCA+CGG 0.693219 4_3:+20402421 None:intergenic
CTGTGAGCTTAAGAACACAA+AGG 0.694006 4_3:-20404481 None:intergenic
GTAGCAATGCTGATAAACAG+AGG 0.712390 4_3:-20402585 None:intergenic
ATATGATAACCTTACATTCG+GGG 0.715139 4_3:+20403311 Msa0633950:CDS
ACTACATTGACAAAGTGAGG+CGG 0.739143 4_3:+20403349 Msa0633950:CDS
GACAACGAGTTTGAAAACGG+CGG 0.794970 4_3:-20402548 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATGTAAGTAGAAATTTT+CGG + chr4_3:20403265-20403284 Msa0633950:CDS 15.0%
!!! TCTAACAAAATAATTTTGAA+TGG - chr4_3:20403403-20403422 None:intergenic 15.0%
!!! TAGTGAATTTTATTATGTAA+TGG + chr4_3:20403437-20403456 Msa0633950:CDS 15.0%
!!! ATATTTATTTGTTAAGTAAG+TGG - chr4_3:20403559-20403578 None:intergenic 15.0%
!! ATTGAAAGATAAAATTGAAA+AGG + chr4_3:20404142-20404161 Msa0633950:CDS 15.0%
!! TAAAATTGAAAAGGAAAATA+TGG + chr4_3:20404151-20404170 Msa0633950:CDS 15.0%
!! AAATGATTTATCAAAAGTAA+TGG + chr4_3:20404682-20404701 Msa0633950:CDS 15.0%
!! ATCAACTGAATTAAAAGTAA+AGG - chr4_3:20402724-20402743 None:intergenic 20.0%
!!! TAAAAAATTTGTTGCATTAG+TGG + chr4_3:20402762-20402781 Msa0633950:intron 20.0%
!!! GAAAAAAGTATTTTACTCTA+GGG + chr4_3:20402995-20403014 Msa0633950:CDS 20.0%
!!! ATTTGGCTTTAAAAGATTTT+TGG - chr4_3:20403052-20403071 None:intergenic 20.0%
!!! ATTTTATTATGTAATGGATG+TGG + chr4_3:20403443-20403462 Msa0633950:CDS 20.0%
!! TATACAAGCAATAATTCTAT+TGG - chr4_3:20404192-20404211 None:intergenic 20.0%
!! ATTTGAATCTCAATTTCAAA+AGG + chr4_3:20404223-20404242 Msa0633950:CDS 20.0%
!! AGATATACACTTATTAAAAG+TGG + chr4_3:20404566-20404585 Msa0633950:CDS 20.0%
!!! TTGATAAATCATTTTCAGTA+TGG - chr4_3:20404676-20404695 None:intergenic 20.0%
! AATCAGAACATATAAGGTTA+TGG - chr4_3:20402490-20402509 None:intergenic 25.0%
! GAACAAAATCAGAACATATA+AGG - chr4_3:20402496-20402515 None:intergenic 25.0%
! GGAAATCAAATTTCAATTGA+TGG - chr4_3:20402622-20402641 None:intergenic 25.0%
! TCACATAAATGAAAATTGTG+AGG + chr4_3:20402828-20402847 Msa0633950:CDS 25.0%
! CATGAAATTTAATTCTACAG+AGG + chr4_3:20402891-20402910 Msa0633950:CDS 25.0%
! AAGAATTATGCAAGATGTTT+AGG + chr4_3:20402931-20402950 Msa0633950:CDS 25.0%
!!! GGAAAAAAGTATTTTACTCT+AGG + chr4_3:20402994-20403013 Msa0633950:CDS 25.0%
!!! TGGAACAATTACTATTTCTA+AGG + chr4_3:20403125-20403144 Msa0633950:CDS 25.0%
!!! CTAAAATATCTTGATTTGCT+TGG - chr4_3:20403183-20403202 None:intergenic 25.0%
! ATATAGACCCTAGTATAAAA+AGG + chr4_3:20403214-20403233 Msa0633950:CDS 25.0%
!!! TCGTTTACGAAATGTTTTTT+GGG + chr4_3:20403476-20403495 Msa0633950:CDS 25.0%
!!! AGGATACTAAAACTTTTTCT+TGG + chr4_3:20403658-20403677 Msa0633950:CDS 25.0%
!! ATGAACTTCATGATAATGAA+TGG + chr4_3:20403949-20403968 Msa0633950:CDS 25.0%
!! ATGATAATGAATGGTTGAAA+GGG + chr4_3:20403958-20403977 Msa0633950:CDS 25.0%
!! ATTGCTTGTATAAGTCATTT+TGG + chr4_3:20404200-20404219 Msa0633950:CDS 25.0%
!! AAACTCTTTGAATTTTGCAT+TGG - chr4_3:20404255-20404274 None:intergenic 25.0%
! TTGTGTTACAGAAAGTAAAA+AGG + chr4_3:20404346-20404365 Msa0633950:CDS 25.0%
! AGTTAAAGACATAATGTTCA+AGG + chr4_3:20404379-20404398 Msa0633950:CDS 25.0%
!! ATGCTGTTTATTTGAGTTTA+AGG + chr4_3:20404439-20404458 Msa0633950:CDS 25.0%
!! TGCTGTTTATTTGAGTTTAA+GGG + chr4_3:20404440-20404459 Msa0633950:CDS 25.0%
! TAGAATAGAGAACTTAAAAC+TGG + chr4_3:20404706-20404725 Msa0633950:CDS 25.0%
AACATATAAGGTTATGGAGA+AGG - chr4_3:20402484-20402503 None:intergenic 30.0%
! CAAATTTCAATTGATGGTTG+AGG - chr4_3:20402616-20402635 None:intergenic 30.0%
TTGAACTGGATGCTAAAATA+GGG - chr4_3:20402643-20402662 None:intergenic 30.0%
GAATTAAAAGTAAAGGCAAC+AGG - chr4_3:20402717-20402736 None:intergenic 30.0%
AAATGAAGATGATGAACCTA+AGG + chr4_3:20402864-20402883 Msa0633950:CDS 30.0%
TTAAATTTCATGCCAACCTT+AGG - chr4_3:20402883-20402902 None:intergenic 30.0%
!! TATGCAAGATGTTTAGGTTT+TGG + chr4_3:20402937-20402956 Msa0633950:CDS 30.0%
TAGCACAATCAGTACAAAAA+AGG + chr4_3:20402963-20402982 Msa0633950:CDS 30.0%
AGCACAATCAGTACAAAAAA+GGG + chr4_3:20402964-20402983 Msa0633950:CDS 30.0%
! CCAATCTCCTTTTTATACTA+GGG - chr4_3:20403224-20403243 None:intergenic 30.0%
! TCCAATCTCCTTTTTATACT+AGG - chr4_3:20403225-20403244 None:intergenic 30.0%
TAGTATAAAAAGGAGATTGG+AGG + chr4_3:20403224-20403243 Msa0633950:CDS 30.0%
! AAGTAGAAATTTTCGGTCTA+TGG + chr4_3:20403272-20403291 Msa0633950:CDS 30.0%
GGATATGATAACCTTACATT+CGG + chr4_3:20403309-20403328 Msa0633950:CDS 30.0%
GATATGATAACCTTACATTC+GGG + chr4_3:20403310-20403329 Msa0633950:CDS 30.0%
ATATGATAACCTTACATTCG+GGG + chr4_3:20403311-20403330 Msa0633950:CDS 30.0%
!!! GTCGTTTACGAAATGTTTTT+TGG + chr4_3:20403475-20403494 Msa0633950:CDS 30.0%
TGATGATTTACTCCAACAAA+AGG - chr4_3:20403597-20403616 None:intergenic 30.0%
TTTGTTGGAGTAAATCATCA+TGG + chr4_3:20403597-20403616 Msa0633950:CDS 30.0%
CTTGTGGCATATAATGAAAA+AGG + chr4_3:20403803-20403822 Msa0633950:CDS 30.0%
! CAAAATACTTTTGCATGATG+CGG + chr4_3:20403866-20403885 Msa0633950:CDS 30.0%
TTTGAGCATAAGTGATTTCA+TGG + chr4_3:20403899-20403918 Msa0633950:CDS 30.0%
! CATGATAATGAATGGTTGAA+AGG + chr4_3:20403957-20403976 Msa0633950:CDS 30.0%
!!! AGTATGAACGCATTTTTTGA+TGG + chr4_3:20404065-20404084 Msa0633950:CDS 30.0%
CTTGGCAATATATAACAAGA+GGG - chr4_3:20404524-20404543 None:intergenic 30.0%
TCTTGGCAATATATAACAAG+AGG - chr4_3:20404525-20404544 None:intergenic 30.0%
! CTTGTTATATATTGCCAAGA+TGG + chr4_3:20404525-20404544 Msa0633950:CDS 30.0%
CTTGAACTGGATGCTAAAAT+AGG - chr4_3:20402644-20402663 None:intergenic 35.0%
CTTACTATTGTCTCTTGAAC+TGG - chr4_3:20402657-20402676 None:intergenic 35.0%
AGTACAAAAAAGGGAGATGA+TGG + chr4_3:20402973-20402992 Msa0633950:CDS 35.0%
! TGTGTTTTCATGCTAGGATT+TGG - chr4_3:20403069-20403088 None:intergenic 35.0%
! TTACAGTGTGTTTTCATGCT+AGG - chr4_3:20403075-20403094 None:intergenic 35.0%
TAGCATGAAAACACACTGTA+AGG + chr4_3:20403074-20403093 Msa0633950:CDS 35.0%
AATGTATGTGTGTCTTCAGA+TGG + chr4_3:20403105-20403124 Msa0633950:CDS 35.0%
CCCTAGTATAAAAAGGAGAT+TGG + chr4_3:20403221-20403240 Msa0633950:CDS 35.0%
TACTTACATTAATCCCAGCT+TGG - chr4_3:20403257-20403276 None:intergenic 35.0%
GAAACTACATTGACAAAGTG+AGG + chr4_3:20403346-20403365 Msa0633950:CDS 35.0%
TTTCTTGGTTGTTCACTACT+TGG + chr4_3:20403673-20403692 Msa0633950:CDS 35.0%
! GAGAGTCTACTCAATTTTTC+TGG - chr4_3:20403831-20403850 None:intergenic 35.0%
TAAGTGATTTCATGGAGAAG+TGG + chr4_3:20403907-20403926 Msa0633950:CDS 35.0%
AAGTGATTTCATGGAGAAGT+GGG + chr4_3:20403908-20403927 Msa0633950:CDS 35.0%
! TGTATGTGAGAGACACATTT+TGG + chr4_3:20404012-20404031 Msa0633950:CDS 35.0%
! GTATGTGAGAGACACATTTT+GGG + chr4_3:20404013-20404032 Msa0633950:CDS 35.0%
! CAAAGAGTTTCAAGTAGAAG+TGG + chr4_3:20404265-20404284 Msa0633950:CDS 35.0%
! TCATGCTTTCTTTGAGAGAA+TGG + chr4_3:20404304-20404323 Msa0633950:CDS 35.0%
GTTATATATTGCCAAGATGG+AGG + chr4_3:20404528-20404547 Msa0633950:CDS 35.0%
TTAATATCCTTCCTCCATCT+TGG - chr4_3:20404542-20404561 None:intergenic 35.0%
!!! AGTGGTTTTGATCAATTAGC+CGG + chr4_3:20404584-20404603 Msa0633950:CDS 35.0%
TGAAGAAGCAAATCCGTAAA+AGG - chr4_3:20404648-20404667 None:intergenic 35.0%
!! AGTTTTTCAGAGATCACTGA+AGG + chr4_3:20404746-20404765 Msa0633950:CDS 35.0%
! TTAGAAGCCTTCCTTTTTGA+AGG - chr4_3:20404842-20404861 None:intergenic 35.0%
ATTGTGAAAAAGAAGCTTGC+TGG + chr4_3:20404872-20404891 Msa0633950:CDS 35.0%
CACTTATCAAAGTCAAGAAG+AGG + chr4_3:20404928-20404947 Msa0633950:CDS 35.0%
GTTCAACAACACAAACTCAC+CGG + chr4_3:20402512-20402531 Msa0633950:CDS 40.0%
GACGACAACGAGTTTGAAAA+CGG - chr4_3:20402554-20402573 None:intergenic 40.0%
GTAGCAATGCTGATAAACAG+AGG - chr4_3:20402588-20402607 None:intergenic 40.0%
GAAGATGATGAACCTAAGGT+TGG + chr4_3:20402868-20402887 Msa0633950:CDS 40.0%
TACTCACATAGCTTCTTGCA+CGG - chr4_3:20403026-20403045 None:intergenic 40.0%
TCTATGGTTGTTGAAGCAAG+TGG + chr4_3:20403288-20403307 Msa0633950:CDS 40.0%
! CATCTTTTTCCCCGAATGTA+AGG - chr4_3:20403323-20403342 None:intergenic 40.0%
ACTACATTGACAAAGTGAGG+CGG + chr4_3:20403349-20403368 Msa0633950:CDS 40.0%
! AGGGCTGCTTATGAGTATTT+TGG + chr4_3:20403513-20403532 Msa0633950:CDS 40.0%
! TGTTAAGTAAGTGGTGTCGA+AGG - chr4_3:20403550-20403569 None:intergenic 40.0%
! ATGCCTTTTGCTCCTTTTGT+TGG + chr4_3:20403582-20403601 Msa0633950:CDS 40.0%
ACTCCAACAAAAGGAGCAAA+AGG - chr4_3:20403588-20403607 None:intergenic 40.0%
AGTATCCTCCTTCGACAATA+GGG - chr4_3:20403646-20403665 None:intergenic 40.0%
TAGTATCCTCCTTCGACAAT+AGG - chr4_3:20403647-20403666 None:intergenic 40.0%
ACTTGGTTAGAATGTATGCG+TGG + chr4_3:20403690-20403709 Msa0633950:CDS 40.0%
CTTGGTTAGAATGTATGCGT+GGG + chr4_3:20403691-20403710 Msa0633950:CDS 40.0%
TGGTCAGTAATTATGGCTTG+TGG - chr4_3:20403723-20403742 None:intergenic 40.0%
ACAAGCCATAATTACTGACC+AGG + chr4_3:20403722-20403741 Msa0633950:CDS 40.0%
TCCATCCTGGTCAGTAATTA+TGG - chr4_3:20403730-20403749 None:intergenic 40.0%
AGCAATGAAGAATGCAGTTG+AGG + chr4_3:20403749-20403768 Msa0633950:CDS 40.0%
AATGTGTCTCTCACATACAC+AGG - chr4_3:20404011-20404030 None:intergenic 40.0%
! TGCTTTCTTTGAGAGAATGG+AGG + chr4_3:20404307-20404326 Msa0633950:CDS 40.0%
CTGTGAGCTTAAGAACACAA+AGG - chr4_3:20404484-20404503 None:intergenic 40.0%
TATATTGCCAAGATGGAGGA+AGG + chr4_3:20404532-20404551 Msa0633950:CDS 40.0%
!! AAGAAGCTTGCTGGAAAGAA+AGG + chr4_3:20404881-20404900 Msa0633950:CDS 40.0%
!!! CACGGCACACGTTTTGATTT+TGG + chr4_3:20402439-20402458 Msa0633950:CDS 45.0%
TTGAAAACGGCGGTGAATGT+TGG - chr4_3:20402541-20402560 None:intergenic 45.0%
GACAACGAGTTTGAAAACGG+CGG - chr4_3:20402551-20402570 None:intergenic 45.0%
TTGGAGGTTAATGACCAAGC+TGG + chr4_3:20403240-20403259 Msa0633950:CDS 45.0%
TGGAGGTTAATGACCAAGCT+GGG + chr4_3:20403241-20403260 Msa0633950:CDS 45.0%
CATGGTCAGTCAGTTCTGTT+AGG + chr4_3:20403615-20403634 Msa0633950:CDS 45.0%
GCCATAATTACTGACCAGGA+TGG + chr4_3:20403726-20403745 Msa0633950:CDS 45.0%
CATTCTTCATTGCTCCATCC+TGG - chr4_3:20403743-20403762 None:intergenic 45.0%
TGTTTGATGAGCGTCATCGT+TGG + chr4_3:20403982-20404001 Msa0633950:CDS 45.0%
GTTTGATGAGCGTCATCGTT+GGG + chr4_3:20403983-20404002 Msa0633950:CDS 45.0%
ACGCTGCAACTCTTCATTTC+CGG - chr4_3:20404606-20404625 None:intergenic 45.0%
! AAAGCCTGCGATGCCTTTTA+CGG + chr4_3:20404632-20404651 Msa0633950:CDS 45.0%
!! AAATCCGTAAAAGGCATCGC+AGG - chr4_3:20404639-20404658 None:intergenic 45.0%
CTGTAGCGACTCGAAGTAAA+GGG + chr4_3:20404810-20404829 Msa0633950:CDS 45.0%
!! GAAGCCTTCCTTTTTGAAGG+AGG - chr4_3:20404839-20404858 None:intergenic 45.0%
TTTGGGCAGATGCGAGATGT+AGG + chr4_3:20403493-20403512 Msa0633950:CDS 50.0%
TTGGGCAGATGCGAGATGTA+GGG + chr4_3:20403494-20403513 Msa0633950:CDS 50.0%
TATGCCACAAGCACCATCGA+TGG - chr4_3:20403794-20403813 None:intergenic 50.0%
! GTGAGAGACACATTTTGGGC+TGG + chr4_3:20404017-20404036 Msa0633950:CDS 50.0%
CCTTTACTTCGAGTCGCTAC+AGG - chr4_3:20404812-20404831 None:intergenic 50.0%
CCTGTAGCGACTCGAAGTAA+AGG + chr4_3:20404809-20404828 Msa0633950:CDS 50.0%
!! AAGGGCGTCCTCCTTCAAAA+AGG + chr4_3:20404828-20404847 Msa0633950:CDS 50.0%
GCGTCCTCCTTCAAAAAGGA+AGG + chr4_3:20404832-20404851 Msa0633950:CDS 50.0%
!! CGGCGGTGAATGTTGGTAAC+CGG - chr4_3:20402534-20402553 None:intergenic 55.0%
AAAGTGAGGCGGCTTCGACT+TGG + chr4_3:20403360-20403379 Msa0633950:CDS 55.0%
AAGTGAGGCGGCTTCGACTT+GGG + chr4_3:20403361-20403380 Msa0633950:CDS 55.0%
AGGTTGCGCCCTATTGTCGA+AGG + chr4_3:20403635-20403654 Msa0633950:CDS 55.0%
TTGCGCCCTATTGTCGAAGG+AGG + chr4_3:20403638-20403657 Msa0633950:CDS 55.0%
ATGGCGAGCTTTCGGGAAGA+CGG - chr4_3:20403775-20403794 None:intergenic 55.0%
ACCATCGATGGCGAGCTTTC+GGG - chr4_3:20403782-20403801 None:intergenic 55.0%
CACCATCGATGGCGAGCTTT+CGG - chr4_3:20403783-20403802 None:intergenic 55.0%
TCCCGAAAGCTCGCCATCGA+TGG + chr4_3:20403778-20403797 Msa0633950:CDS 60.0%
CTCGCCATCGATGGTGCTTG+TGG + chr4_3:20403787-20403806 Msa0633950:CDS 60.0%
!! AGGCGGCTTCGACTTGGGAC+AGG + chr4_3:20403366-20403385 Msa0633950:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 20402425 20404994 20402425 ID=Msa0633950;Name=Msa0633950
chr4_3 mRNA 20402425 20404994 20402425 ID=Msa0633950-mRNA-1;Parent=Msa0633950;Name=Msa0633950-mRNA-1;_AED=0.25;_eAED=0.37;_QI=0|0|0|0.5|1|1|2|0|819
chr4_3 exon 20402425 20402671 20402425 ID=Msa0633950-mRNA-1:exon:5431;Parent=Msa0633950-mRNA-1
chr4_3 exon 20402782 20404994 20402782 ID=Msa0633950-mRNA-1:exon:5432;Parent=Msa0633950-mRNA-1
chr4_3 CDS 20402425 20402671 20402425 ID=Msa0633950-mRNA-1:cds;Parent=Msa0633950-mRNA-1
chr4_3 CDS 20402782 20404994 20402782 ID=Msa0633950-mRNA-1:cds;Parent=Msa0633950-mRNA-1
Gene Sequence

>Msa0633950

ATGGAAGAGACAATCACGGCACACGTTTTGATTTTGGACAATCACAGTACAATCTTCCTTCTCCATAACCTTATATGTTCTGATTTTGTTCAACAACACAAACTCACCGGTTACCAACATTCACCGCCGTTTTCAAACTCGTTGTCGTCATTCTCTTATGCCTCTGTTTATCAGCATTGCTACTCTAACCTCAACCATCAATTGAAATTTGATTTCCCTATTTTAGCATCCAGTTCAAGAGACAATATGGAGAAAACTTTTAGATCTTTTGAAGAAGATGAAGAAGAGAGTGATCACATAAATGAAAATTGTGAGGATAATTTAACAGAAAATGAAGATGATGAACCTAAGGTTGGCATGAAATTTAATTCTACAGAGGAAGTTGACAAATATTACAAGAATTATGCAAGATGTTTAGGTTTTGGAGTTAGCACAATCAGTACAAAAAAGGGAGATGATGGAAAAAAGTATTTTACTCTAGGGTGTAACCGTGCAAGAAGCTATGTGAGTAAGTCCAAAAATCTTTTAAAGCCAAATCCTAGCATGAAAACACACTGTAAGGCTAGAATAAATGTATGTGTGTCTTCAGATGGAACAATTACTATTTCTAAGGTTGCTCTTGAACATAATCATGACTTAATAAGTCCAAGCAAATCAAGATATTTTAGATGCAACAAGAATATAGACCCTAGTATAAAAAGGAGATTGGAGGTTAATGACCAAGCTGGGATTAATGTAAGTAGAAATTTTCGGTCTATGGTTGTTGAAGCAAGTGGATATGATAACCTTACATTCGGGGAAAAAGATGTTAGAAACTACATTGACAAAGTGAGGCGGCTTCGACTTGGGACAGGAGACGCTGAAGCCATTCAAAATTATTTTGTTAGAATGCAAAGACAAAATAGTGAATTTTATTATGTAATGGATGTGGATGACAAAAGTCGTTTACGAAATGTTTTTTGGGCAGATGCGAGATGTAGGGCTGCTTATGAGTATTTTGGTGAAGTAATAACCTTCGACACCACTTACTTAACAAATAAATATGACATGCCTTTTGCTCCTTTTGTTGGAGTAAATCATCATGGTCAGTCAGTTCTGTTAGGTTGCGCCCTATTGTCGAAGGAGGATACTAAAACTTTTTCTTGGTTGTTCACTACTTGGTTAGAATGTATGCGTGGGCTTGCTCCACAAGCCATAATTACTGACCAGGATGGAGCAATGAAGAATGCAGTTGAGGCCGTCTTCCCGAAAGCTCGCCATCGATGGTGCTTGTGGCATATAATGAAAAAGGTTCCAGAAAAATTGAGTAGACTCTCTAACTATGAGTCTATCAAAATACTTTTGCATGATGCGGTGTATGATTCTTTGAGCATAAGTGATTTCATGGAGAAGTGGGAAAAAATGATTGAATGTTATGAACTTCATGATAATGAATGGTTGAAAGGGTTGTTTGATGAGCGTCATCGTTGGGTTCCTGTGTATGTGAGAGACACATTTTGGGCTGGAATGTCAACAACACAACGAAGTGAAAGTATGAACGCATTTTTTGATGGATATGTGCACTCAAAGACATCATTGAAGCAATTTGTTGAACAATATGATAATGCATTGAAAGATAAAATTGAAAAGGAAAATATGGCTGATTTTAGTTCATCCAATAGAATTATTGCTTGTATAAGTCATTTTGGATTTGAATCTCAATTTCAAAAGGCATATACCAATGCAAAATTCAAAGAGTTTCAAGTAGAAGTGGCTTCTATGATGTACTGTCATGCTTTCTTTGAGAGAATGGAGGCTTTGAACTTAACATATTGTGTTACAGAAAGTAAAAAGGTATTTGATACAGTTAAAGACATAATGTTCAAGGTATTCTTCAATGAGAAAGACTTTGAAATACGATGTACATGCTGTTTATTTGAGTTTAAGGGTATTTTATGTAGACATATCCTTTGTGTTCTTAAGCTCACAGCTAAAACAGAGTCAGTGCCCTCTTGTTATATATTGCCAAGATGGAGGAAGGATATTAAGCGAAGATATACACTTATTAAAAGTGGTTTTGATCAATTAGCCGGAAATGAAGAGTTGCAGCGTGTAGCTAAAGCCTGCGATGCCTTTTACGGATTTGCTTCTTCATGTATCCATACTGAAAATGATTTATCAAAAGTAATGGATAGAATAGAGAACTTAAAACTGGAGATGACATGTCAAGAAAGTTTTTCAGAGATCACTGAAGGAGATAACTTAGTTCAAAATCAGATGACTAAAATTCTTGATCCTGTAGCGACTCGAAGTAAAGGGCGTCCTCCTTCAAAAAGGAAGGCTTCTAAAGTTGATCAAATTGTGAAAAAGAAGCTTGCTGGAAAGAAAGGACAAAAAAGCAACAAAAAAAGTAACACTTATCAAAGTCAAGAAGAGGTAATCTTGAAATTTTCAAGTTTATTTTATCTCCTTTACACGTAA
Protein sequence

>Msa0633950

MEETITAHVLILDNHSTIFLLHNLICSDFVQQHKLTGYQHSPPFSNSLSSFSYASVYQHCYSNLNHQLKFDFPILASSSRDNMEKTFRSFEEDEEESDHINENCEDNLTENEDDEPKVGMKFNSTEEVDKYYKNYARCLGFGVSTISTKKGDDGKKYFTLGCNRARSYVSKSKNLLKPNPSMKTHCKARINVCVSSDGTITISKVALEHNHDLISPSKSRYFRCNKNIDPSIKRRLEVNDQAGINVSRNFRSMVVEASGYDNLTFGEKDVRNYIDKVRRLRLGTGDAEAIQNYFVRMQRQNSEFYYVMDVDDKSRLRNVFWADARCRAAYEYFGEVITFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSKEDTKTFSWLFTTWLECMRGLAPQAIITDQDGAMKNAVEAVFPKARHRWCLWHIMKKVPEKLSRLSNYESIKILLHDAVYDSLSISDFMEKWEKMIECYELHDNEWLKGLFDERHRWVPVYVRDTFWAGMSTTQRSESMNAFFDGYVHSKTSLKQFVEQYDNALKDKIEKENMADFSSSNRIIACISHFGFESQFQKAYTNAKFKEFQVEVASMMYCHAFFERMEALNLTYCVTESKKVFDTVKDIMFKVFFNEKDFEIRCTCCLFEFKGILCRHILCVLKLTAKTESVPSCYILPRWRKDIKRRYTLIKSGFDQLAGNEELQRVAKACDAFYGFASSCIHTENDLSKVMDRIENLKLEMTCQESFSEITEGDNLVQNQMTKILDPVATRSKGRPPSKRKASKVDQIVKKKLAGKKGQKSNKKSNTYQSQEEVILKFSSLFYLLYT*