AlfalfaGEDB | Msa0634360

M. sativa cultivar zhongmu-4 / Msa0634360

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0097159 GO:0120025 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634360	XP_039688539.1	75.325	154	15	2	1	133	1	152	9.05e-72	224

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634360	sp\|Q9FJK3\|AGL80_ARATH	41.935	155	68	3	1	134	1	154	4.23e-30	113

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634360	A0A396I1B6	75.325	154	15	2	1	133	1	152	4.32e-72	224

TFs/TRs:

Gene ID	Type	Classification
Msa0634360	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634360	MtrunA17_Chr4g0013041	75.325	154	15	2	1	133	1	152	8.33e-76	224
Msa0634360	MtrunA17_Chr4g0014321	66.234	154	31	1	1	133	1	154	4.95e-65	197
Msa0634360	MtrunA17_Chr8g0351751	58.228	158	43	2	1	135	1	158	3.07e-52	165
Msa0634360	MtrunA17_Chr4g0014031	53.205	156	52	2	1	135	1	156	4.24e-49	157
Msa0634360	MtrunA17_Chr3g0107381	47.742	155	59	2	1	134	1	154	1.02e-43	142
Msa0634360	MtrunA17_Chr3g0110131	53.548	155	50	2	1	134	1	154	4.81e-43	142
Msa0634360	MtrunA17_Chr4g0014131	49.359	156	58	2	1	135	1	156	6.86e-43	141
Msa0634360	MtrunA17_Chr4g0014121	52.564	156	53	1	1	135	29	184	6.72e-40	132
Msa0634360	MtrunA17_Chr2g0296831	57.025	121	31	1	1	100	1	121	2.25e-34	115
Msa0634360	MtrunA17_Chr3g0107331	39.869	153	66	3	3	132	2	151	7.00e-31	108
Msa0634360	MtrunA17_Chr3g0107361	36.842	152	74	2	3	133	2	152	7.92e-29	103
Msa0634360	MtrunA17_Chr3g0107351	36.842	152	74	2	3	133	2	152	6.14e-28	100
Msa0634360	MtrunA17_Chr5g0420351	36.538	156	76	3	1	135	1	154	1.04e-26	97.4
Msa0634360	MtrunA17_Chr3g0107341	35.526	152	76	2	3	133	2	152	5.73e-26	95.9
Msa0634360	MtrunA17_Chr3g0107461	30.769	156	87	1	1	135	1	156	1.87e-23	89.4
Msa0634360	MtrunA17_Chr7g0218151	35.256	156	78	4	1	135	1	154	4.82e-22	85.9
Msa0634360	MtrunA17_Chr1g0197261	31.613	155	83	3	1	134	1	153	5.94e-21	82.8
Msa0634360	MtrunA17_Chr5g0432691	33.117	154	81	3	1	133	1	153	8.77e-21	82.4
Msa0634360	MtrunA17_Chr2g0283941	31.613	155	85	1	1	134	1	155	7.99e-20	82.0
Msa0634360	MtrunA17_Chr1g0197321	29.032	155	88	2	1	134	1	154	1.04e-19	79.7
Msa0634360	MtrunA17_Chr1g0188681	31.169	154	83	3	1	133	1	152	1.21e-19	79.3
Msa0634360	MtrunA17_Chr1g0197461	33.333	147	75	3	3	128	2	146	2.56e-19	78.6
Msa0634360	MtrunA17_Chr4g0012761	33.117	154	80	4	1	133	1	152	3.29e-19	78.6
Msa0634360	MtrunA17_Chr1g0193311	32.692	156	82	4	1	135	1	154	6.87e-18	75.1
Msa0634360	MtrunA17_Chr4g0031421	34.314	102	67	0	32	133	25	126	4.04e-16	70.1
Msa0634360	MtrunA17_Chr2g0296851	50.000	82	41	0	53	134	1	82	1.19e-15	67.8
Msa0634360	MtrunA17_Chr3g0105791	31.373	102	70	0	32	133	25	126	1.23e-14	66.2
Msa0634360	MtrunA17_Chr1g0188661	29.487	156	87	4	1	135	1	154	2.60e-14	65.9
Msa0634360	MtrunA17_Chr2g0296861	30.968	155	85	2	1	134	1	154	3.82e-14	65.1
Msa0634360	MtrunA17_Chr3g0106501	28.155	103	74	0	33	135	26	128	2.09e-12	60.5
Msa0634360	MtrunA17_Chr3g0106421	33.333	102	68	0	33	134	26	127	2.38e-12	61.6
Msa0634360	MtrunA17_Chr1g0188651	30.519	154	82	4	1	133	1	150	7.29e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634360	AT5G48670.1	41.935	155	68	3	1	134	1	154	4.30e-31	113
Msa0634360	AT5G26630.1	37.419	155	75	4	1	134	1	154	4.29e-26	98.2
Msa0634360	AT1G65300.1	36.601	153	75	2	3	134	2	153	4.33e-24	94.4
Msa0634360	AT1G65330.1	35.948	153	76	3	3	134	2	153	2.19e-20	84.3
Msa0634360	AT5G26650.1	35.526	152	76	2	4	134	2	152	3.66e-20	84.7
Msa0634360	AT3G05860.2	34.000	150	77	2	1	129	1	149	1.13e-19	81.3
Msa0634360	AT5G27960.1	34.641	153	78	2	4	135	2	153	1.15e-19	83.2
Msa0634360	AT3G05860.3	34.000	150	77	2	1	129	1	149	4.75e-19	80.5
Msa0634360	AT3G05860.1	34.000	150	77	2	1	129	1	149	7.57e-19	80.1
Msa0634360	AT1G31630.1	29.801	151	85	3	3	133	2	151	7.81e-16	72.8
Msa0634360	AT1G31640.1	28.667	150	86	2	3	132	2	150	1.55e-15	72.4
Msa0634360	AT5G27810.1	46.970	66	35	0	35	100	28	93	2.91e-15	67.4
Msa0634360	AT5G26580.1	33.333	144	82	3	1	134	1	140	3.55e-14	68.2
Msa0634360	AT2G28700.1	28.947	152	82	3	1	129	1	149	8.63e-13	64.3
Msa0634360	AT5G06500.1	28.931	159	80	3	1	133	1	152	3.23e-11	59.3

Find 31 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAACAATGACTCGGAAAA+AGG	0.208892	4_3:+21503109	None:intergenic
TCTCTTGGTTCACCATTTCC+TGG	0.286300	4_3:-21503355	None:intergenic
TTGTGTCTTCAGTTTCTCTT+TGG	0.304045	4_3:-21503405	None:intergenic
TTTGTGTCATATAAGTCTCT+TGG	0.418787	4_3:-21503370	None:intergenic
ATGAGCCTCAACCGGAGATC+TGG	0.422183	4_3:+21503267	Msa0634360:intron
GGAGATCTGGCCATCACCAC+GGG	0.435650	4_3:+21503280	Msa0634360:CDS
GAAGGACATTAGTAGAAGGT+TGG	0.444167	4_3:+21503556	Msa0634360:CDS
AATTGAAGGACATTAGTAGA+AGG	0.455635	4_3:+21503552	Msa0634360:CDS
CGTGGTGATGGCCAGATCTC+CGG	0.460734	4_3:-21503278	None:intergenic
TTGATTCAAAACATCTTTGT+TGG	0.470119	4_3:+21503516	Msa0634360:CDS
GTGGGAGAAGAGCCAGGAAA+TGG	0.489343	4_3:+21503343	Msa0634360:CDS
GCAGAAGGGTCTATTGAAGA+AGG	0.498190	4_3:+21503187	Msa0634360:CDS
GTCTGAGTGGGAGAAGAGCC+AGG	0.507130	4_3:+21503337	Msa0634360:CDS
TGGACCATATGAGCCTCAAC+CGG	0.517498	4_3:+21503259	Msa0634360:intron
GATGGCCAGATCTCCGGTTG+AGG	0.525272	4_3:-21503272	None:intergenic
ATGTTTCAATGTCTTTGTGA+AGG	0.533884	4_3:+21503464	Msa0634360:CDS
AGTGCAGAATAATATCTCCT+TGG	0.537754	4_3:+21503493	Msa0634360:CDS
GTATGGACTCCCCGTGGTGA+TGG	0.556504	4_3:-21503290	None:intergenic
AACCTACAATAAAAGGCAGA+AGG	0.564320	4_3:+21503172	Msa0634360:CDS
ATTCAGAACAATGTCTGAGT+GGG	0.569100	4_3:+21503325	Msa0634360:CDS
GACATTAGTAGAAGGTTGGA+AGG	0.584170	4_3:+21503560	Msa0634360:CDS
TATGACACAAAGGGTTCTGA+AGG	0.587149	4_3:+21503382	Msa0634360:CDS
CGGAGATCTGGCCATCACCA+CGG	0.587483	4_3:+21503279	Msa0634360:CDS
AGAGACTTATATGACACAAA+GGG	0.592057	4_3:+21503373	Msa0634360:CDS
AAGAGACTTATATGACACAA+AGG	0.616813	4_3:+21503372	Msa0634360:CDS
ACCTACAATAAAAGGCAGAA+GGG	0.621576	4_3:+21503173	Msa0634360:CDS
AATTCAGAACAATGTCTGAG+TGG	0.644068	4_3:+21503324	Msa0634360:CDS
TGTCAACTATGAGAAAGAGA+TGG	0.660566	4_3:+21503433	Msa0634360:CDS
AAGAGAAACTGAAGACACAA+AGG	0.673063	4_3:+21503408	Msa0634360:CDS
GAGATCTGGCCATCACCACG+GGG	0.753289	4_3:+21503281	Msa0634360:CDS
AGCACTGTATGGACTCCCCG+TGG	0.777500	4_3:-21503296	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAAAAACAACCTACAATAAA+AGG	+	chr4_3:21503165-21503184	Msa0634360:CDS	25.0%
!	TTGATTCAAAACATCTTTGT+TGG	+	chr4_3:21503516-21503535	Msa0634360:CDS	25.0%
!!	TACTAATGTCCTTCAATTTT+TGG	-	chr4_3:21503550-21503569	None:intergenic	25.0%
	TTTGTGTCATATAAGTCTCT+TGG	-	chr4_3:21503373-21503392	None:intergenic	30.0%
	AAGAGACTTATATGACACAA+AGG	+	chr4_3:21503372-21503391	Msa0634360:CDS	30.0%
	AGAGACTTATATGACACAAA+GGG	+	chr4_3:21503373-21503392	Msa0634360:CDS	30.0%
	ATGTTTCAATGTCTTTGTGA+AGG	+	chr4_3:21503464-21503483	Msa0634360:CDS	30.0%
!!	AAGATGTTTTGAATCAACCA+AGG	-	chr4_3:21503513-21503532	None:intergenic	30.0%
	GTTGATTGACCAAAAATTGA+AGG	+	chr4_3:21503538-21503557	Msa0634360:CDS	30.0%
	AATTGAAGGACATTAGTAGA+AGG	+	chr4_3:21503552-21503571	Msa0634360:CDS	30.0%
	AACCTACAATAAAAGGCAGA+AGG	+	chr4_3:21503172-21503191	Msa0634360:CDS	35.0%
	ACCTACAATAAAAGGCAGAA+GGG	+	chr4_3:21503173-21503192	Msa0634360:CDS	35.0%
	TATGAACTAAGCACTCTATG+TGG	+	chr4_3:21503212-21503231	Msa0634360:intron	35.0%
!!!	TGAATTTTGAGAGCACTGTA+TGG	-	chr4_3:21503310-21503329	None:intergenic	35.0%
	AATTCAGAACAATGTCTGAG+TGG	+	chr4_3:21503324-21503343	Msa0634360:CDS	35.0%
	ATTCAGAACAATGTCTGAGT+GGG	+	chr4_3:21503325-21503344	Msa0634360:CDS	35.0%
	TTGTGTCTTCAGTTTCTCTT+TGG	-	chr4_3:21503408-21503427	None:intergenic	35.0%
	AAGAGAAACTGAAGACACAA+AGG	+	chr4_3:21503408-21503427	Msa0634360:CDS	35.0%
	TGTCAACTATGAGAAAGAGA+TGG	+	chr4_3:21503433-21503452	Msa0634360:CDS	35.0%
	AGTGCAGAATAATATCTCCT+TGG	+	chr4_3:21503493-21503512	Msa0634360:CDS	35.0%
!	ACCCTTCTGCCTTTTATTGT+AGG	-	chr4_3:21503177-21503196	None:intergenic	40.0%
	CTCATATGGTCCATAGACAA+TGG	-	chr4_3:21503252-21503271	None:intergenic	40.0%
!	TATGACACAAAGGGTTCTGA+AGG	+	chr4_3:21503382-21503401	Msa0634360:CDS	40.0%
	GAAGGACATTAGTAGAAGGT+TGG	+	chr4_3:21503556-21503575	Msa0634360:CDS	40.0%
	GACATTAGTAGAAGGTTGGA+AGG	+	chr4_3:21503560-21503579	Msa0634360:CDS	40.0%
	GCAGAAGGGTCTATTGAAGA+AGG	+	chr4_3:21503187-21503206	Msa0634360:CDS	45.0%
	GAAGCTTGAGCCATTGTCTA+TGG	+	chr4_3:21503239-21503258	Msa0634360:intron	45.0%
	TCTCTTGGTTCACCATTTCC+TGG	-	chr4_3:21503358-21503377	None:intergenic	45.0%
	TGGACCATATGAGCCTCAAC+CGG	+	chr4_3:21503259-21503278	Msa0634360:intron	50.0%
	ATCTCCGGTTGAGGCTCATA+TGG	-	chr4_3:21503266-21503285	None:intergenic	50.0%
	ATGAGCCTCAACCGGAGATC+TGG	+	chr4_3:21503267-21503286	Msa0634360:intron	55.0%
	GTGGGAGAAGAGCCAGGAAA+TGG	+	chr4_3:21503343-21503362	Msa0634360:CDS	55.0%
	GATGGCCAGATCTCCGGTTG+AGG	-	chr4_3:21503275-21503294	None:intergenic	60.0%
!!	CGTGGTGATGGCCAGATCTC+CGG	-	chr4_3:21503281-21503300	None:intergenic	60.0%
	CGGAGATCTGGCCATCACCA+CGG	+	chr4_3:21503279-21503298	Msa0634360:CDS	60.0%
	GGAGATCTGGCCATCACCAC+GGG	+	chr4_3:21503280-21503299	Msa0634360:CDS	60.0%
	GAGATCTGGCCATCACCACG+GGG	+	chr4_3:21503281-21503300	Msa0634360:CDS	60.0%
	GTATGGACTCCCCGTGGTGA+TGG	-	chr4_3:21503293-21503312	None:intergenic	60.0%
	AGCACTGTATGGACTCCCCG+TGG	-	chr4_3:21503299-21503318	None:intergenic	60.0%
	GTCTGAGTGGGAGAAGAGCC+AGG	+	chr4_3:21503337-21503356	Msa0634360:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	21503116	21503589	21503116	ID=Msa0634360;Name=Msa0634360
chr4_3	mRNA	21503116	21503589	21503116	ID=Msa0634360-mRNA-1;Parent=Msa0634360;Name=Msa0634360-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0\|0\|1\|0\|0\|2\|0\|136
chr4_3	exon	21503116	21503208	21503116	ID=Msa0634360-mRNA-1:exon:5590;Parent=Msa0634360-mRNA-1
chr4_3	exon	21503272	21503589	21503272	ID=Msa0634360-mRNA-1:exon:5591;Parent=Msa0634360-mRNA-1
chr4_3	CDS	21503116	21503208	21503116	ID=Msa0634360-mRNA-1:cds;Parent=Msa0634360-mRNA-1
chr4_3	CDS	21503272	21503589	21503272	ID=Msa0634360-mRNA-1:cds;Parent=Msa0634360-mRNA-1

Gene Sequence

>Msa0634360

ATGACTCGGAAAAAGGTGAAACTTGCTTTCATTGTCAATGATGCTTCTAGAAAAACAACCTACAATAAAAGGCAGAAGGGTCTATTGAAGAAGCCTCAACCGGAGATCTGGCCATCACCACGGGGAGTCCATACAGTGCTCTCAAAATTCAGAACAATGTCTGAGTGGGAGAAGAGCCAGGAAATGGTGAACCAAGAGACTTATATGACACAAAGGGTTCTGAAGGCCAAAGAGAAACTGAAGACACAAAGGCATGTCAACTATGAGAAAGAGATGGTTATGCTCATGTTTCAATGTCTTTGTGAAGGCAAATTAGTGCAGAATAATATCTCCTTGGTTGATTCAAAACATCTTTGTTGGTTGATTGACCAAAAATTGAAGGACATTAGTAGAAGGTTGGAAGGTCAGTAA

Protein sequence

>Msa0634360

MTRKKVKLAFIVNDASRKTTYNKRQKGLLKKPQPEIWPSPRGVHTVLSKFRTMSEWEKSQEMVNQETYMTQRVLKAKEKLKTQRHVNYEKEMVMLMFQCLCEGKLVQNNISLVDSKHLCWLIDQKLKDISRRLEGQ*