AlfalfaGEDB | Msa0634720

M. sativa cultivar zhongmu-4 / Msa0634720

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0097159 GO:0120025 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634720	XP_003605474.1	72.851	221	43	2	1	209	1	216	1.58e-100	308

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634720	sp\|Q9FJK3\|AGL80_ARATH	55.844	154	67	1	1	154	1	153	1.56e-43	157

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634720	G7JM29	72.851	221	43	2	1	209	1	216	7.57e-101	308

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TFs/TRs:

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Gene ID	Type	Classification
Msa0634720	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634720	MtrunA17_Chr4g0014031	72.851	221	43	2	1	209	1	216	1.46e-104	308
Msa0634720	MtrunA17_Chr4g0014131	67.949	234	40	2	1	212	1	221	1.45e-100	297
Msa0634720	MtrunA17_Chr4g0014321	59.276	221	81	1	1	212	1	221	2.51e-89	269
Msa0634720	MtrunA17_Chr4g0014101	83.234	167	27	1	221	386	181	347	4.18e-89	272
Msa0634720	MtrunA17_Chr8g0351751	58.515	229	75	3	1	209	1	229	6.07e-84	256
Msa0634720	MtrunA17_Chr4g0013041	61.538	195	67	2	1	195	1	187	8.46e-84	254
Msa0634720	MtrunA17_Chr4g0014121	80.255	157	31	0	1	157	29	185	1.26e-80	245
Msa0634720	MtrunA17_Chr3g0107381	47.222	216	98	4	1	211	1	205	3.73e-61	196
Msa0634720	MtrunA17_Chr4g0014161	66.242	157	53	0	223	379	178	334	1.28e-60	199
Msa0634720	MtrunA17_Chr4g0014141	64.458	166	57	2	223	386	175	340	1.76e-60	198

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0634720	AT5G48670.1	55.844	154	67	1	1	154	1	153	1.58e-44	157
Msa0634720	AT1G33970.1	50.000	156	78	0	221	376	175	330	1.61e-43	154
Msa0634720	AT1G33970.2	50.000	156	78	0	221	376	175	330	1.61e-43	154
Msa0634720	AT1G33970.4	50.000	156	78	0	221	376	175	330	1.61e-43	154
Msa0634720	AT1G33970.5	50.000	156	78	0	221	376	175	330	1.61e-43	154
Msa0634720	AT5G26630.1	45.026	191	94	2	1	181	1	190	6.18e-39	139
Msa0634720	AT1G65330.1	44.643	168	83	2	3	161	2	168	1.12e-38	140
Msa0634720	AT1G65300.1	46.053	152	81	1	3	154	2	152	8.91e-38	137
Msa0634720	AT5G26650.1	35.909	220	116	6	5	209	3	212	3.12e-33	127
Msa0634720	AT5G27960.1	35.455	220	117	6	5	209	3	212	5.85e-33	126

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Find 90 sgRNAs with CRISPR-Local

Find 349 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTAAGGAAGAATCTTG+AGG	0.621998	4_3:+22382979	Msa0634720:CDS
AAATAAGTGTGCAGTAGTCA+CGG	0.567614	4_3:-22383149	None:intergenic
AACAAAGGGTCTATTGAAAA+AGG	0.314581	4_3:+22378739	Msa0634720:CDS
AACATCTAGGAGTGAGGAAA+TGG	0.393870	4_3:+22379159	Msa0634720:CDS
AACCATTACCACCTCGCTTG+CGG	0.557199	4_3:-22383018	None:intergenic
AACGATTCCCACACAAAGAG+AGG	0.567121	4_3:-22381437	None:intergenic
AAGAGACTTTCTTGACACAA+AGG	0.587821	4_3:+22378924	Msa0634720:CDS
AAGGATGAAAAGAAGCAATC+TGG	0.460191	4_3:+22381483	Msa0634720:CDS
AATTCTTTACGGTGTAATCT+TGG	0.404119	4_3:+22379425	Msa0634720:CDS
ACAATGAAATACGAAAACTA+AGG	0.582337	4_3:+22382966	Msa0634720:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAAGGGTCTATTGAAAA+AGG	+	chr4_3:22378739-22378758	Msa0634720:CDS	30.0%
GTTGATTGACCATAATTTGA+AGG	+	chr4_3:22379090-22379109	Msa0634720:CDS	30.0%
TACCAATGTCCTTCAAATTA+TGG	-	chr4_3:22379102-22379121	None:intergenic	30.0%
AATTGAGAAAAGATCCTTCA+AGG	-	chr4_3:22379334-22379353	None:intergenic	30.0%
AATTCTTTACGGTGTAATCT+TGG	+	chr4_3:22379425-22379444	Msa0634720:CDS	30.0%
TTATTTCCTGTCTGTAGTTT+CGG	+	chr4_3:22379453-22379472	Msa0634720:intron	30.0%
AACCAATGTTCAATACAAGA+AGG	+	chr4_3:22379603-22379622	Msa0634720:intron	30.0%
ATCCTTCTTGTATTGAACAT+TGG	-	chr4_3:22379608-22379627	None:intergenic	30.0%
TTGTATAGAGTTTGTCTCTT+TGG	-	chr4_3:22380546-22380565	None:intergenic	30.0%
TTGTGAAAGTGACTCAAAAT+TGG	-	chr4_3:22380850-22380869	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	22378668	22383231	22378668	ID=Msa0634720;Name=Msa0634720
chr4_3	mRNA	22378668	22383231	22378668	ID=Msa0634720-mRNA-1;Parent=Msa0634720;Name=Msa0634720-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0.5\|0.6\|0.8\|0.5\|0.4\|5\|177\|386
chr4_3	exon	22378668	22379295	22378668	ID=Msa0634720-mRNA-1:exon:5784;Parent=Msa0634720-mRNA-1
chr4_3	exon	22379415	22379446	22379415	ID=Msa0634720-mRNA-1:exon:5785;Parent=Msa0634720-mRNA-1
chr4_3	exon	22381432	22381596	22381432	ID=Msa0634720-mRNA-1:exon:5786;Parent=Msa0634720-mRNA-1
chr4_3	exon	22381683	22381817	22381683	ID=Msa0634720-mRNA-1:exon:5787;Parent=Msa0634720-mRNA-1
chr4_3	exon	22382854	22383231	22382854	ID=Msa0634720-mRNA-1:exon:5788;Parent=Msa0634720-mRNA-1
chr4_3	CDS	22378668	22379295	22378668	ID=Msa0634720-mRNA-1:cds;Parent=Msa0634720-mRNA-1
chr4_3	CDS	22379415	22379446	22379415	ID=Msa0634720-mRNA-1:cds;Parent=Msa0634720-mRNA-1
chr4_3	CDS	22381432	22381596	22381432	ID=Msa0634720-mRNA-1:cds;Parent=Msa0634720-mRNA-1
chr4_3	CDS	22381683	22381817	22381683	ID=Msa0634720-mRNA-1:cds;Parent=Msa0634720-mRNA-1
chr4_3	CDS	22382854	22383054	22382854	ID=Msa0634720-mRNA-1:cds;Parent=Msa0634720-mRNA-1
chr4_3	three_prime_UTR	22383055	22383231	22383055	ID=Msa0634720-mRNA-1:three_prime_utr;Parent=Msa0634720-mRNA-1

Gene Sequence

>Msa0634720

ATGGCTAGAAGTAAGGTTAAACTCGCCTTCATTGTCAATGATGCTGCAAGGAAAGCAACATACAAAAAAAGAACAAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTGTGCCATAGTGTATGGCTCTTATGAGCCTCAACCTGAGATTTGGCCTTCACCAGAGGGGGTCCAATCTGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGCTGAACCAAGAGACTTTCTTGACACAAAGGATTCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGGGAGCAAGAGATGACAATACTCATGTCTCATTGTCTCAATGAAGGTAGAGTTGTGCACGACAATATGTCAACGGTTGATTTGAGTTATCTTACTTGGTTGATTGACCATAATTTGAAGGACATTGGTAGACGTTTGGCGGCAACAGTGCCACCACCACCTCCAACAACATCTAGGAGTGAGGAAATGGAAATTGTGGGTCATGGCCATGTTGGGATACCTATTAATGATGATGAAATTATGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCACGAGACAGTGCCCTTTGATACAATCGTAATTCTTTACGGTGTAATCTTGCAAACCCTCTCTTTGTGTGGGAATCGTTGTGTTCTCTTAGACAACACGACAAAGGATGAAAAGAAGCAATCTGGACAAGTTCAACAACTTCTCTCGCTTGTAAGCTTGATAGTTTCACAGAATGGTGGGCAACCCTATACAGATGAGTTATTTGCAGAACTAAAGAAAGGAGCAATGGAACTGCGTAGCGGGCAAAGAAAGATTGATTCCTTGGAAGGACAGTCAGAAGAACAAATATCAGAGCTTAAAAAGCATATGAAGCAAACATATGAGGATCAGCTAAAACATATTACTGAGATGATTGAGTCAAAGTTGAAGGAGTCAACTAAAAGGCTGGAGAAACAACTGAAAGAAGAGCATGCTGCAAGATTAAAAGCAGAAGACAATGCAAAGTTGGCTCAAAAGAAATCAGACAATGAAATACGAAAACTAAGGAAGAATCTTGAGGAGGCGCATGAAGAACTCCGCAAGCGAGGTGGTAATGGTTGTGCCATTCTATGA

Protein sequence

>Msa0634720

MARSKVKLAFIVNDAARKATYKKRTKGLLKKVAELSTLCGIEACAIVYGSYEPQPEIWPSPEGVQSVLSKFMTMHEFQKCKKMLNQETFLTQRILKAEEKLKKQRKENREQEMTILMSHCLNEGRVVHDNMSTVDLSYLTWLIDHNLKDIGRRLAATVPPPPPTTSRSEEMEIVGHGHVGIPINDDEIMQSQLLMDSMVAGNVHETVPFDTIVILYGVILQTLSLCGNRCVLLDNTTKDEKKQSGQVQQLLSLVSLIVSQNGGQPYTDELFAELKKGAMELRSGQRKIDSLEGQSEEQISELKKHMKQTYEDQLKHITEMIESKLKESTKRLEKQLKEEHAARLKAEDNAKLAQKKSDNEIRKLRKNLEEAHEELRKRGGNGCAIL*