AlfalfaGEDB | Msa0641230

M. sativa cultivar zhongmu-4 / Msa0641230

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0641230	XP_003605290.2	92.522	575	38	3	1	573	1	572	0.0	1022

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0641230	sp\|Q8H186\|PBL1_ARATH	38.907	311	173	7	193	491	45	350	4.51e-61	210

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0641230	MtrunA17_Chr4g0012871	92.522	575	38	3	1	573	1	572	0.0	1022
Msa0641230	MtrunA17_Chr7g0244861	57.022	591	173	14	1	572	1	529	0.0	557
Msa0641230	MtrunA17_Chr4g0008091	55.556	603	183	13	1	584	1	537	0.0	537
Msa0641230	MtrunA17_Chr5g0416311	38.710	310	166	7	193	488	60	359	4.29e-62	210
Msa0641230	MtrunA17_Chr8g0370461	36.283	339	175	10	168	488	62	377	8.37e-61	207
Msa0641230	MtrunA17_Chr5g0408941	39.934	303	156	8	197	486	80	369	1.52e-60	206
Msa0641230	MtrunA17_Chr7g0216551	38.658	313	176	6	184	486	99	405	1.76e-59	205
Msa0641230	MtrunA17_Chr3g0136421	40.461	304	163	8	197	490	70	365	3.22e-59	202
Msa0641230	MtrunA17_Chr5g0416011	37.748	302	172	5	194	485	121	416	1.67e-58	203
Msa0641230	MtrunA17_Chr4g0031601	36.513	304	187	3	197	494	80	383	1.75e-58	202

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0641230	AT3G08760.3	61.394	373	117	6	155	523	142	491	2.72e-152	449
Msa0641230	AT3G08760.1	61.394	373	117	6	155	523	142	491	2.72e-152	449
Msa0641230	AT3G08760.2	61.394	373	117	6	155	523	142	491	2.72e-152	449
Msa0641230	AT3G55450.1	38.907	311	173	7	193	491	45	350	4.59e-62	210
Msa0641230	AT3G55450.2	38.907	311	173	7	193	491	82	387	2.37e-61	209
Msa0641230	AT2G28940.2	37.143	315	179	6	192	492	85	394	3.21e-60	207
Msa0641230	AT1G69790.2	39.610	308	160	7	193	486	57	352	1.17e-59	203
Msa0641230	AT5G15080.1	38.284	303	171	5	194	486	127	423	1.19e-59	206
Msa0641230	AT1G69790.1	39.610	308	160	7	193	486	68	363	2.37e-59	202
Msa0641230	AT2G28940.1	40.000	265	145	4	237	492	16	275	3.85e-58	198

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Find 133 sgRNAs with CRISPR-Local

Find 358 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCGAGCAGTTTACAG+AGG	0.649620	4_3:+41215987	None:intergenic
AAACTCCATACATTGCTCTT+TGG	0.375668	4_3:+41215177	None:intergenic
AAAGCAGCAAGAAAAGTAGC+TGG	0.544647	4_3:-41214994	Msa0641230:CDS
AAAGGAAGCTTTATATATGG+TGG	0.501181	4_3:+41216308	None:intergenic
AAAGGTACTGGACTAGATAC+AGG	0.526208	4_3:+41216489	None:intergenic
AACTCGGTTAATTGGGTTAA+GGG	0.396104	4_3:+41216809	None:intergenic
AAGAGCAATGTATGGAGTTT+TGG	0.280984	4_3:-41215174	Msa0641230:CDS
AAGAGGAGAAGGAAGCATTG+TGG	0.672769	4_3:+41214708	None:intergenic
AATCCATGATCACCGACAAC+TGG	0.432463	4_3:+41215043	None:intergenic
AATGCAAAGCTATCAGGATA+TGG	0.322315	4_3:-41215515	Msa0641230:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTTGTCTTCATGTTCTTAA+AGG	+	chr4_3:41214643-41214662	None:intergenic	30.0%
TTGCAAATACATGCTCATTA+TGG	+	chr4_3:41214695-41214714	None:intergenic	30.0%
TTCAGTATGAAGAACAAGAA+CGG	-	chr4_3:41215048-41215067	Msa0641230:CDS	30.0%
GTTTCTTCATCAATCTATCT+CGG	+	chr4_3:41215082-41215101	None:intergenic	30.0%
CATATGAAAGTAATGTAGCA+AGG	+	chr4_3:41215384-41215403	None:intergenic	30.0%
TAACTTTCTTGCATGAAGAA+GGG	-	chr4_3:41215782-41215801	Msa0641230:intron	30.0%
TTTATTATTATCACCTGGAG+AGG	+	chr4_3:41215808-41215827	None:intergenic	30.0%
TCTCAAGATCATACTAGATA+CGG	-	chr4_3:41216688-41216707	Msa0641230:CDS	30.0%
CTTTGTCTTCATGTTCTTAA+AGG	+	chr4_3:41214643-41214662	None:intergenic	30.0%
TTGCAAATACATGCTCATTA+TGG	+	chr4_3:41214695-41214714	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	41214624	41216991	41214624	ID=Msa0641230;Name=Msa0641230
chr4_3	mRNA	41214624	41216991	41214624	ID=Msa0641230-mRNA-1;Parent=Msa0641230;Name=Msa0641230-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|0\|0\|1\|1\|1\|4\|0\|584
chr4_3	exon	41216245	41216991	41216245	ID=Msa0641230-mRNA-1:exon:9280;Parent=Msa0641230-mRNA-1
chr4_3	exon	41215802	41216065	41215802	ID=Msa0641230-mRNA-1:exon:9279;Parent=Msa0641230-mRNA-1
chr4_3	exon	41215469	41215588	41215469	ID=Msa0641230-mRNA-1:exon:9278;Parent=Msa0641230-mRNA-1
chr4_3	exon	41214624	41215247	41214624	ID=Msa0641230-mRNA-1:exon:9277;Parent=Msa0641230-mRNA-1
chr4_3	CDS	41216245	41216991	41216245	ID=Msa0641230-mRNA-1:cds;Parent=Msa0641230-mRNA-1
chr4_3	CDS	41215802	41216065	41215802	ID=Msa0641230-mRNA-1:cds;Parent=Msa0641230-mRNA-1
chr4_3	CDS	41215469	41215588	41215469	ID=Msa0641230-mRNA-1:cds;Parent=Msa0641230-mRNA-1
chr4_3	CDS	41214624	41215247	41214624	ID=Msa0641230-mRNA-1:cds;Parent=Msa0641230-mRNA-1

Gene Sequence

>Msa0641230

ATGGGATGTTTCCCTGCCTTTAAGAACATGAAGACAAAGTGTGAAAATTCCAATTCAGAAATTAGCAGCCATAATGAGCATGTATTTGCAACAACGCCTCAACTTCAAGCTTCTCGCTCGTTGCACTCTGATCCTCGAAGTTCTAAGGCTAGCATGATATCCCTTAACCCAATTAACCGAGTTGCCAACAACAGAACACGGGCGTTGTCTGCTCCGTCAGCTCTAGCCTCAGTTGAGCATGAGGAACAAGAAATATCGAGAGTTTTAGCGAGATTAATGAAGGAGCCGCGTTCGCCTATTCCACAACCCTTACCTCTTCCATCCCCTCAAGGAAATGGTCCGTTGAAGTTAAGGATGGCTGGTGGTCCTGTATATGCTTCTGGTTCAGCTCTTGTTGCAGCAGAACAAGATGGCTTGGAAACATTTCAGTATGAAGAACAAGAACGGTCAAAAAACCGAGATAGATTGATGAAGAAACAGCCTGTATCTAGTCCAGTACCTTTACCGCTTCCAACTCCTCAAGGAGGTACATTGAAGACTACTACTGGCAGCTCTAAGTTAGGAACATCTATTGATTCGGTTCGTTACTTTCGGCATGAGGAAATTGCTGCTGCTTGCCGCAATTTCTCTTCTGATCGATGCATGTCAGAATGTCTTTATTCCACCATATATAAAGCTTCCTTTTTTAATGATGCTTCAAGTGAGAAGCTTAAAGCCACTGTCACACGCCTTCACTCATCGACTCAGGGCTTGAGAGAATTCATGAATGAGGTTAATACTCTTTCGAATTTGCAACATCCAAACCTCTGTAAACTGCTCGGATTTTATGCAAGAGATGGCTTTGAGTCTGAACCAAGGATGTTGGTTTACGAGAGGCTAATCAATGGGAGCTTGGACCGCCTGTTGTTTGGAAAATCTGACGGCCCTTCAATTGATTGGAACGCAAGAATGAAAATTGCCATCTGTGTTGCACAAGGTCTAACTTTCTTGCATGAAGAAGGGCCTCTCCAGGCAATGTATAATGATTTTTCAGCAGTAAACATACAGATTGATGTAGATTTCAATGCAAAGCTATCAGGATATGGTTTTGTTGGACATGTTGCTGAGGAAGAGATTTCAAGCAGTTCAACTGCAGCAGCAAATCTTTCAGTGGAGACATTGAAAAAAGGAATGCTTACTCCAAAGAGCAATGTATGGAGTTTTGGAATTGTTCTTCTGGAGCTACTTACCGGTAGAAAGCATTTTGACAGTCATCTACCGAAGAATGAGAGGAACTTAGTCAAGTGGTGTCGGCCTTACCTAGCCGACGATTTCCAGTTGTCGGTGATCATGGATTCACAACTCAAAGGTCAGTTTCCGCCTAAAGCAGCAAGAAAAGTAGCTGGAATTGTACAACAATGCCTTCAAATGGAGCCATCAGAAAGACCAACCATGAGAGCCGTTGTTGAGAGTCTCAAGATCATACTAGATACGGAGTACCCTAGTTGGTTCCCACTGCAAGATCCAGCAGCCATGTATGGAAGACATGCGTCAAGATCACCAAGCGTTGATGGTATCATTAATGCACCTCGATTGAGTTTCTCTCCTCCATTACTACCATCAAAAGCCAGAACATCTGTTTCACATCCTGCAAGATGGTCAACTGTGTCCACAATGCTTCCTTCTCCTCTTGCTCGTTCCTCCAATGTTTCTACTGAGGAGCTCACTAGGCAGGAAAGTACAATGTCATCATCCTCAGTGTCCAAGAAAGCTTAG

Protein sequence

>Msa0641230

MGCFPAFKNMKTKCENSNSEISSHNEHVFATTPQLQASRSLHSDPRSSKASMISLNPINRVANNRTRALSAPSALASVEHEEQEISRVLARLMKEPRSPIPQPLPLPSPQGNGPLKLRMAGGPVYASGSALVAAEQDGLETFQYEEQERSKNRDRLMKKQPVSSPVPLPLPTPQGGTLKTTTGSSKLGTSIDSVRYFRHEEIAAACRNFSSDRCMSECLYSTIYKASFFNDASSEKLKATVTRLHSSTQGLREFMNEVNTLSNLQHPNLCKLLGFYARDGFESEPRMLVYERLINGSLDRLLFGKSDGPSIDWNARMKIAICVAQGLTFLHEEGPLQAMYNDFSAVNIQIDVDFNAKLSGYGFVGHVAEEEISSSSTAAANLSVETLKKGMLTPKSNVWSFGIVLLELLTGRKHFDSHLPKNERNLVKWCRPYLADDFQLSVIMDSQLKGQFPPKAARKVAGIVQQCLQMEPSERPTMRAVVESLKIILDTEYPSWFPLQDPAAMYGRHASRSPSVDGIINAPRLSFSPPLLPSKARTSVSHPARWSTVSTMLPSPLARSSNVSTEELTRQESTMSSSSVSKKA*