AlfalfaGEDB | Msa0643630

M. sativa cultivar zhongmu-4 / Msa0643630

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643630	AES88739.2	80.050	401	35	7	1	396	1	361	0.0	622

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643630	sp\|Q9SIB6\|PBL6_ARATH	47.059	391	178	9	1	374	1	379	6.52e-104	316

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643630	G7JSJ4	80.050	401	35	7	1	396	1	361	0.0	622

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643630	MtrunA17_Chr4g0030311	70.250	400	35	8	1	396	1	320	0.0	511
Msa0643630	MtrunA17_Chr4g0030301	81.022	274	51	1	123	395	2	275	1.08e-162	457
Msa0643630	MtrunA17_Chr4g0030471	63.663	344	105	5	24	363	19	346	1.54e-144	414
Msa0643630	MtrunA17_Chr2g0327471	46.803	391	172	9	1	374	1	372	2.06e-106	319
Msa0643630	MtrunA17_Chr2g0327511	53.770	305	128	6	92	383	96	400	7.44e-102	307
Msa0643630	MtrunA17_Chr7g0263521	50.814	307	140	5	88	383	57	363	1.44e-100	303
Msa0643630	MtrunA17_Chr2g0327531	53.716	296	126	5	90	374	29	324	3.25e-100	302
Msa0643630	MtrunA17_Chr4g0074651	51.039	337	144	8	51	370	68	400	3.91e-100	303
Msa0643630	MtrunA17_Chr1g0181821	50.000	318	145	6	88	394	58	372	1.38e-99	301
Msa0643630	MtrunA17_Chr2g0286281	50.943	318	141	6	88	394	73	386	8.52e-99	303

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643630	AT2G28590.1	47.059	391	178	9	1	374	1	379	6.63e-105	316
Msa0643630	AT1G07870.1	52.050	317	139	6	80	383	77	393	8.80e-102	308
Msa0643630	AT1G07870.2	52.050	317	139	6	80	383	77	393	1.26e-100	309
Msa0643630	AT1G61860.1	50.462	325	149	6	83	396	65	388	5.77e-99	300
Msa0643630	AT5G18610.1	53.401	294	126	5	88	370	67	360	9.25e-99	303
Msa0643630	AT5G18610.2	53.401	294	126	5	88	370	67	360	9.25e-99	303
Msa0643630	AT5G18610.3	53.401	294	126	5	88	370	67	360	9.25e-99	303
Msa0643630	AT3G20530.1	49.521	313	146	5	78	378	56	368	2.22e-98	298
Msa0643630	AT5G02800.1	52.721	294	128	5	92	374	61	354	2.20e-97	295
Msa0643630	AT3G24790.1	52.027	296	131	5	86	370	29	324	2.48e-96	292

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Find 69 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTCCTTGACCCACAA+AGG	0.578327	4_3:+46753094	None:intergenic
AAAACCTTTGTGGGTCAAGG+AGG	0.630356	4_3:-46753098	Msa0643630:CDS
AAATCAGATATTTACTGCTT+TGG	0.320357	4_3:-46752233	Msa0643630:CDS
AATTGCCACTGAAATAGCAA+AGG	0.604974	4_3:-46752462	Msa0643630:CDS
ACATGATGCAGCAATGGCAA+TGG	0.584701	4_3:+46751570	None:intergenic
ACTCAATGTCTCCACTTCAG+CGG	0.679849	4_3:+46752783	None:intergenic
ACTTTCTGCTTCATCAACCT+TGG	0.449138	4_3:+46751471	None:intergenic
AGACAATTTCAAAACCTTTG+TGG	0.488527	4_3:-46753108	Msa0643630:CDS
AGCGGTGGCGACAGTGGTGA+TGG	0.611206	4_3:+46754263	None:intergenic
AGGGTTGCTTCAAACACAAC+AGG	0.467221	4_3:-46753057	Msa0643630:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCATGCAATTGATATTGTTA+TGG	-	chr4_3:46751525-46751544	Msa0643630:CDS	30.0%
AGTTGGTCACTTAAACTAAT+GGG	-	chr4_3:46752179-46752198	Msa0643630:CDS	30.0%
GTTAGAGATGAAACATTAAC+TGG	+	chr4_3:46752234-46752253	None:intergenic	30.0%
ACCAATTGAAAACATGAAAC+TGG	-	chr4_3:46752260-46752279	Msa0643630:CDS	30.0%
ATATAAGAAATGAGGCGATA+CGG	-	chr4_3:46752532-46752551	Msa0643630:intron	30.0%
AGACAATTTCAAAACCTTTG+TGG	-	chr4_3:46752587-46752606	Msa0643630:intron	30.0%
GACAATTTCAAAACCTTTGT+GGG	-	chr4_3:46752588-46752607	Msa0643630:intron	30.0%
ACTACTAAACATTCTAGTCA+TGG	+	chr4_3:46752757-46752776	None:intergenic	30.0%
TTAAGGTCACAGTATATCAT+AGG	+	chr4_3:46753291-46753310	None:intergenic	30.0%
GATCCAGATTATACAAAAAC+AGG	-	chr4_3:46753426-46753445	Msa0643630:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	46751356	46754361	46751356	ID=Msa0643630;Name=Msa0643630
chr4_3	mRNA	46751356	46754361	46751356	ID=Msa0643630-mRNA-1;Parent=Msa0643630;Name=Msa0643630-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|0.75\|0.6\|1\|1\|1\|5\|70\|396
chr4_3	exon	46751356	46751677	46751356	ID=Msa0643630-mRNA-1:exon:11001;Parent=Msa0643630-mRNA-1
chr4_3	exon	46752151	46752524	46752151	ID=Msa0643630-mRNA-1:exon:11000;Parent=Msa0643630-mRNA-1
chr4_3	exon	46752671	46752860	46752671	ID=Msa0643630-mRNA-1:exon:10999;Parent=Msa0643630-mRNA-1
chr4_3	exon	46753058	46753290	46753058	ID=Msa0643630-mRNA-1:exon:10998;Parent=Msa0643630-mRNA-1
chr4_3	exon	46754220	46754361	46754220	ID=Msa0643630-mRNA-1:exon:10997;Parent=Msa0643630-mRNA-1
chr4_3	CDS	46754220	46754361	46754220	ID=Msa0643630-mRNA-1:cds;Parent=Msa0643630-mRNA-1
chr4_3	CDS	46753058	46753290	46753058	ID=Msa0643630-mRNA-1:cds;Parent=Msa0643630-mRNA-1
chr4_3	CDS	46752671	46752860	46752671	ID=Msa0643630-mRNA-1:cds;Parent=Msa0643630-mRNA-1
chr4_3	CDS	46752151	46752524	46752151	ID=Msa0643630-mRNA-1:cds;Parent=Msa0643630-mRNA-1
chr4_3	CDS	46751426	46751677	46751426	ID=Msa0643630-mRNA-1:cds;Parent=Msa0643630-mRNA-1
chr4_3	three_prime_UTR	46751356	46751425	46751356	ID=Msa0643630-mRNA-1:three_prime_utr;Parent=Msa0643630-mRNA-1

Gene Sequence

>Msa0643630

ATGAATTGCTTTTGTATCCGTCCCAACAAAACTAACACACAGAATGTCACCACCACTGCAAGTAACACCGAGAATACCATCACCACTGTCGCCACCGCTTCCACTCCCGCTCCCCCACGTAGCTTTCTTTCAAAGATTTTGGGAAAGGGAAAGGGTAAACTTGAATCCAAAGTGAATAAGAAAAAAGAGGATTCTGCAACTTCTAGAATTGAAGAACATCCTACATATACTGAAATTGTGAATTTAAATATAAGAAATGAGGCGATACGGAAATTTTGTTTTGAAGAACTTAGTGCAGCAACAGACAATTTCAAAACCTTTGTGGGTCAAGGAGGTTTTGGCGGAGTTTACTTAGGGTTGCTTCAAACACAACAGGTCGTGGCTATAAAGCGACGTGACTCAAATAGTGAACAAGGAACCCGTGAATTTACCGCTGAAGTGGAGACATTGAGTAATGTAAGCCACAAAAATATTGTCCAACTGATTGGATATTGTGCTGAGAAGGAGCACCAGCTATTAGTCTATGAGTACCTGAGGTTGGGATCTTTGGAAGATAATTTGTTAGATCATCGGCGAGGTATTCTTCATTGGGATACAAGAATGAGAATTGCCACTGAAATAGCAAAGGGTGTGGAGTATCTACATGTTAAAATGGATCCTCCTATGATATACTGTGACCTTAAAAGCTCAAATATTTTGTTAGGTGATGGATATGATGTAAAGTTATCTGATTTTGGGTGTGCTAAAGTAGGCCCAGAATATCGTTCAGCAAAGATTTTTGGCACAATTGGATACTTTGATCCAGATTATACAAAAACAGGTATTTTGTCATTCAAATCAGATATTTACTGCTTTGGAGTTGTACTTTTGGAGCTGATCAGTGGTAGAAGAGCTTTTGATGAAACAAGACGTGACAGTGAACCAAATGTTGTTTTATGGGCAAGTCCTTTATTTGAAGATAGATTCGAAGAAATTGTTGACCCATTGCTAGAAGAGAAATATCCTGTGAGAGATTTACAGAAAGCCATTGCCATTGCTGCATCATGTGTTCAAAAGAAAGTTGATGATAGGCCCGATATAAGTCAGATTGTTAAAGACCTCGAAGCTCTAAATAAAATGAAATCCAAGGTTGATGAAGCAGAAAGTTCTGGAGCAGCAGAGAGAAGGGTTGGACAAAGTTCTACAAGTTGA

Protein sequence

>Msa0643630

MNCFCIRPNKTNTQNVTTTASNTENTITTVATASTPAPPRSFLSKILGKGKGKLESKVNKKKEDSATSRIEEHPTYTEIVNLNIRNEAIRKFCFEELSAATDNFKTFVGQGGFGGVYLGLLQTQQVVAIKRRDSNSEQGTREFTAEVETLSNVSHKNIVQLIGYCAEKEHQLLVYEYLRLGSLEDNLLDHRRGILHWDTRMRIATEIAKGVEYLHVKMDPPMIYCDLKSSNILLGDGYDVKLSDFGCAKVGPEYRSAKIFGTIGYFDPDYTKTGILSFKSDIYCFGVVLLELISGRRAFDETRRDSEPNVVLWASPLFEDRFEEIVDPLLEEKYPVRDLQKAIAIAASCVQKKVDDRPDISQIVKDLEALNKMKSKVDEAESSGAAERRVGQSSTS*