AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0645320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645320 MtrunA17_Chr4g0032951 89.977 429 17 4 1 411 1 421 0.0 725
Msa0645320 MtrunA17_Chr2g0329041 51.136 440 136 13 1 407 1 394 4.20e-132 385
Msa0645320 MtrunA17_Chr1g0174271 50.420 238 100 6 182 406 127 359 1.22e-67 219
Msa0645320 MtrunA17_Chr3g0087771 36.667 420 189 16 15 410 21 387 2.09e-50 175
Msa0645320 MtrunA17_Chr7g0258181 40.664 241 115 5 175 407 85 305 5.85e-42 150
Msa0645320 MtrunA17_Chr8g0351881 38.000 250 110 9 182 409 154 380 4.96e-35 133
Msa0645320 MtrunA17_Chr4g0014771 76.316 76 16 1 331 406 106 179 4.71e-33 122
Msa0645320 MtrunA17_Chr1g0213791 66.304 92 30 1 315 406 236 326 1.01e-32 126
Msa0645320 MtrunA17_Chr3g0131771 61.290 62 24 0 345 406 147 208 3.92e-16 79.0
Msa0645320 MtrunA17_Chr4g0074161 56.061 66 28 1 344 409 205 269 6.90e-16 79.0
Msa0645320 MtrunA17_Chr1g0152131 55.385 65 29 0 345 409 260 324 2.22e-15 77.4
Msa0645320 MtrunA17_Chr8g0339091 55.385 65 29 0 345 409 291 355 8.14e-15 76.3
Msa0645320 MtrunA17_Chr3g0144641 58.065 62 26 0 345 406 191 252 8.60e-15 75.5
Msa0645320 MtrunA17_Chr7g0234761 58.065 62 26 0 345 406 317 378 9.28e-15 75.9
Msa0645320 MtrunA17_Chr8g0365941 58.065 62 26 0 345 406 305 366 1.21e-14 75.5
Msa0645320 MtrunA17_Chr1g0190731 53.030 66 30 1 344 409 192 256 2.13e-14 73.9
Msa0645320 MtrunA17_Chr8g0385911 56.452 62 27 0 345 406 145 206 3.31e-14 73.2
Msa0645320 MtrunA17_Chr5g0421111 53.846 65 30 0 345 409 365 429 4.03e-14 73.9
Msa0645320 MtrunA17_Chr4g0035101 56.452 62 27 0 345 406 143 204 4.38e-14 73.2
Msa0645320 MtrunA17_Chr8g0363921 52.308 65 31 0 345 409 151 215 8.93e-14 71.2
Msa0645320 MtrunA17_Chr5g0415291 50.769 65 32 0 345 409 112 176 3.28e-13 68.9
Msa0645320 MtrunA17_Chr6g0484271 46.154 65 35 0 345 409 155 219 1.44e-11 64.7
Msa0645320 MtrunA17_Chr1g0153241 35.238 105 65 2 303 407 45 146 2.43e-11 63.9
Msa0645320 MtrunA17_Chr3g0134741 51.562 64 30 1 344 407 228 290 2.96e-11 64.3
Msa0645320 MtrunA17_Chr1g0152651 50.000 66 32 1 344 409 273 337 3.80e-11 64.3
Msa0645320 MtrunA17_Chr7g0258161 44.615 65 36 0 345 409 173 237 4.92e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645320 AT1G51140.1 39.810 422 183 17 1 407 1 366 8.40e-70 225
Msa0645320 AT2G42280.1 44.628 242 105 7 178 411 125 345 8.81e-53 180
Msa0645320 AT2G42280.3 45.378 238 101 7 178 407 125 341 4.45e-52 178
Msa0645320 AT2G43140.1 66.667 93 30 1 315 406 103 195 1.25e-34 128
Msa0645320 AT1G35460.1 75.342 73 18 0 337 409 173 245 7.42e-34 127
Msa0645320 AT2G43140.2 66.667 93 30 1 315 406 200 292 7.77e-34 128
Msa0645320 AT1G05805.1 65.591 93 30 2 315 406 253 344 2.55e-33 128
Msa0645320 AT4G09180.1 74.648 71 18 0 339 409 178 248 3.42e-30 117
Msa0645320 AT2G42280.2 28.902 346 174 13 25 362 15 296 1.02e-22 97.8
Msa0645320 AT1G35460.2 82.979 47 8 0 337 383 173 219 3.48e-22 94.7
Msa0645320 AT2G24260.2 57.576 66 27 1 344 409 34 98 1.68e-16 79.0
Msa0645320 AT2G24260.1 57.576 66 27 1 344 409 137 201 4.55e-16 79.3
Msa0645320 AT5G58010.1 54.545 66 29 1 344 409 98 162 6.60e-16 78.2
Msa0645320 AT4G34530.1 47.126 87 44 1 323 409 152 236 7.30e-16 78.6
Msa0645320 AT4G30980.1 56.061 66 28 1 344 409 129 193 8.98e-16 78.2
Msa0645320 AT5G50915.3 56.923 65 28 0 345 409 136 200 1.26e-15 77.0
Msa0645320 AT5G50915.4 56.923 65 28 0 345 409 136 200 1.26e-15 77.0
Msa0645320 AT4G30980.2 56.061 66 28 1 344 409 177 241 1.30e-15 78.2
Msa0645320 AT5G50915.1 59.677 62 25 0 345 406 136 197 1.69e-15 77.0
Msa0645320 AT5G50915.2 59.677 62 25 0 345 406 136 197 1.69e-15 77.0
Msa0645320 AT1G25330.1 52.308 65 31 0 345 409 104 168 2.20e-15 75.5
Msa0645320 AT1G68920.3 55.385 65 29 0 345 409 302 366 5.02e-15 77.0
Msa0645320 AT1G68920.1 55.385 65 29 0 345 409 303 367 5.08e-15 77.0
Msa0645320 AT1G68920.4 55.385 65 29 0 345 409 303 367 5.08e-15 77.0
Msa0645320 AT1G68920.2 55.385 65 29 0 345 409 303 367 5.08e-15 77.0
Msa0645320 AT1G26260.5 55.385 65 29 0 345 409 201 265 8.05e-15 75.9
Msa0645320 AT1G26260.4 55.385 65 29 0 345 409 223 287 9.66e-15 75.9
Msa0645320 AT1G26260.1 55.385 65 29 0 345 409 223 287 9.66e-15 75.9
Msa0645320 AT1G26260.6 55.385 65 29 0 345 409 223 287 9.66e-15 75.9
Msa0645320 AT1G26260.2 55.385 65 29 0 345 409 223 287 9.66e-15 75.9
Msa0645320 AT1G26260.3 55.385 65 29 0 345 409 172 236 1.01e-14 75.5
Msa0645320 AT3G07340.2 55.385 65 29 0 345 409 258 322 1.21e-14 75.9
Msa0645320 AT1G10120.2 55.385 65 29 0 345 409 206 270 1.24e-14 75.1
Msa0645320 AT1G10120.1 55.385 65 29 0 345 409 206 270 1.24e-14 75.1
Msa0645320 AT3G07340.1 55.385 65 29 0 345 409 258 322 1.55e-14 75.5
Msa0645320 AT1G73830.2 52.308 65 31 0 345 409 147 211 2.18e-14 73.2
Msa0645320 AT1G73830.1 52.308 65 31 0 345 409 147 211 2.29e-14 73.2
Msa0645320 AT1G18400.2 54.839 62 28 0 345 406 145 206 2.48e-14 72.8
Msa0645320 AT5G48560.2 53.846 65 30 0 345 409 301 365 3.53e-14 74.3
Msa0645320 AT1G18400.1 54.839 62 28 0 345 406 145 206 3.56e-14 72.4
Msa0645320 AT5G48560.1 53.846 65 30 0 345 409 301 365 3.65e-14 74.7
Msa0645320 AT1G59640.1 52.308 65 31 0 345 409 136 200 1.91e-13 70.5
Msa0645320 AT3G23690.1 52.308 65 31 0 345 409 191 255 3.05e-13 71.2
Msa0645320 AT1G59640.2 54.839 62 28 0 345 406 136 197 1.04e-12 69.3
Msa0645320 AT2G18300.2 49.231 65 33 0 345 409 185 249 1.06e-12 69.3
Msa0645320 AT2G18300.1 49.231 65 33 0 345 409 185 249 1.06e-12 69.3
Msa0645320 AT2G18300.3 51.613 62 30 0 345 406 185 246 1.55e-12 68.9
Msa0645320 AT4G36540.2 51.613 62 30 0 345 406 141 202 1.63e-12 68.2
Msa0645320 AT4G36540.1 51.613 62 30 0 345 406 141 202 1.69e-12 68.2
Msa0645320 AT2G42300.1 46.575 73 38 1 338 409 177 249 1.77e-12 68.6
Msa0645320 AT2G42300.4 46.575 73 38 1 338 409 177 249 2.36e-12 67.4
Msa0645320 AT3G57800.2 47.692 65 34 0 345 409 204 268 1.24e-11 66.2
Msa0645320 AT5G62610.2 47.692 65 34 0 345 409 153 217 1.63e-11 65.1
Msa0645320 AT5G62610.1 47.692 65 34 0 345 409 153 217 1.63e-11 65.1
Msa0645320 AT1G27740.1 47.222 72 37 1 340 411 162 232 2.57e-11 63.9

Find 107 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACAACAAAGTAATAATT+TGG 0.127177 4_3:+50275147 Msa0645320:CDS
AGTAATAATTTGGGTAATTA+TGG 0.157665 4_3:+50275157 Msa0645320:CDS
TCACTCTGTTCACTCCTTAT+TGG 0.196524 4_3:+50274001 Msa0645320:five_prime_UTR
GCCAATTCGGCCGTAGTATT+TGG 0.246472 4_3:-50276853 None:intergenic
CATCAGCAACAAATGAATTC+AGG 0.254682 4_3:+50274767 Msa0645320:CDS
TGCCACGTTCCATTCATATT+TGG 0.254794 4_3:+50273908 Msa0645320:five_prime_UTR
ACCCTTTCTGCAATGCTTCT+TGG 0.259998 4_3:-50276952 None:intergenic
CGACCGGTCTTCATTTGATT+TGG 0.266534 4_3:-50275232 None:intergenic
CCCTTTCTGCAATGCTTCTT+GGG 0.277269 4_3:-50276951 None:intergenic
CCAGACTACTTCTGCTCCTT+TGG 0.303630 4_3:+50276813 Msa0645320:CDS
TCAACTACTGAATCAGTTAT+AGG 0.313043 4_3:-50274995 None:intergenic
ATCTTTACCTTATCCATGTT+TGG 0.314679 4_3:-50277478 None:intergenic
CTTCTCAATGGCTGCCAATT+CGG 0.320940 4_3:-50276866 None:intergenic
CGAGTTTCAGCAAAACCTTC+AGG 0.323395 4_3:-50276348 None:intergenic
CAATAAGGGAAAAGAATTAA+AGG 0.332137 4_3:+50277997 Msa0645320:three_prime_UTR
GCCAAATACTACGGCCGAAT+TGG 0.337775 4_3:+50276852 Msa0645320:CDS
CCTAACGGTGAGGCATAGTT+TGG 0.353263 4_3:-50276282 None:intergenic
CTTTCTGTTTCAGGACTTGA+AGG 0.354030 4_3:-50274872 None:intergenic
TTTGCTGCTATGAGAGGAAT+AGG 0.354357 4_3:+50276186 Msa0645320:CDS
TAATGATGTATGAATAATTA+TGG 0.357532 4_3:-50277720 None:intergenic
TATGAGAGGAATAGGAACTA+TGG 0.359228 4_3:+50276194 Msa0645320:CDS
GGTGGTAGTGGTGGTGGTGG+TGG 0.363507 4_3:+50274932 Msa0645320:CDS
GGATTTAAACCTGGAGGAAA+TGG 0.364210 4_3:-50276483 None:intergenic
TCAAACATTAACATTGATAC+TGG 0.367647 4_3:+50275298 Msa0645320:CDS
ATGATATCTGACAATATTAC+TGG 0.368731 4_3:+50276426 Msa0645320:CDS
CCCAAGAAGCATTGCAGAAA+GGG 0.381192 4_3:+50276951 Msa0645320:CDS
TCAACCTCTTCCTTAACTAT+AGG 0.381573 4_3:-50275061 None:intergenic
TTTATCCCGGGTTTCCCCTT+AGG 0.384096 4_3:+50276381 Msa0645320:CDS
TCTCTTGAACCTTCCTATAG+AGG 0.385056 4_3:+50274076 Msa0645320:five_prime_UTR
TATGGTTGTTTGGGAATGGT+GGG 0.390684 4_3:+50278137 Msa0645320:three_prime_UTR
TCACACAAGCAACAGCAATA+AGG 0.392323 4_3:+50277982 Msa0645320:three_prime_UTR
CTGTGGAGGTGCAGCCAATA+AGG 0.395442 4_3:-50274015 None:intergenic
CTATGGTTGTTTGGGAATGG+TGG 0.396240 4_3:+50278136 Msa0645320:three_prime_UTR
GGAAGTGGTGGTAGTGGTGG+TGG 0.399180 4_3:+50274926 Msa0645320:CDS
CACACAAGCAACAGCAATAA+GGG 0.405182 4_3:+50277983 Msa0645320:three_prime_UTR
TCACCGTTAGGAGCTGAAAT+CGG 0.415027 4_3:+50276294 Msa0645320:CDS
CCAAAGGAGCAGAAGTAGTC+TGG 0.416991 4_3:-50276813 None:intergenic
GAAAGTGAAGAAACCTCTAT+AGG 0.438178 4_3:-50274089 None:intergenic
AGACAACAAAGTAATAATTT+GGG 0.443177 4_3:+50275148 Msa0645320:CDS
GCAACAGCTTCCTATAGTTA+AGG 0.446191 4_3:+50275051 Msa0645320:CDS
CGGAGGAAGTATTTATAGAA+AGG 0.446973 4_3:-50277768 None:intergenic
AGTGGTGGTAGTGGTGGTGG+TGG 0.447971 4_3:+50274929 Msa0645320:CDS
TTCATTTGATTTGGCAAAGG+TGG 0.451723 4_3:-50275223 None:intergenic
CACCGTTAGGAGCTGAAATC+GGG 0.453811 4_3:+50276295 Msa0645320:CDS
AAGGTGGTCTTCCTGAATTA+TGG 0.457093 4_3:-50275207 None:intergenic
TTTCTATGGTTGTTTGGGAA+TGG 0.462791 4_3:+50278133 Msa0645320:three_prime_UTR
ATCCTCCCATGTGGTACCTA+AGG 0.463348 4_3:-50276397 None:intergenic
TGATGATCTAGAAACATAGT+TGG 0.464492 4_3:-50274737 None:intergenic
CCAAACTATGCCTCACCGTT+AGG 0.468050 4_3:+50276282 Msa0645320:CDS
ATGAATAGTCTTAGTGGAAG+TGG 0.476450 4_3:+50274911 Msa0645320:CDS
TGCAATGCTTCTTGGGTGAG+TGG 0.478659 4_3:-50276944 None:intergenic
AGGAAGGTTCAAGAGAGAAA+AGG 0.485293 4_3:-50274069 None:intergenic
TAGAGAAGTGAGAATGAGAT+TGG 0.487406 4_3:-50277677 None:intergenic
ATCAGCAACAAATGAATTCA+GGG 0.496532 4_3:+50274768 Msa0645320:CDS
TTGAGAAAACCAAATATGAA+TGG 0.501615 4_3:-50273917 None:intergenic
CACACAACAAATCTGAAAGC+TGG 0.505837 4_3:+50273973 Msa0645320:five_prime_UTR
CTAAAATTAGCGAGCGAATG+AGG 0.505851 4_3:+50277437 Msa0645320:CDS
AGTGGAAGTGGTGGTAGTGG+TGG 0.511352 4_3:+50274923 Msa0645320:CDS
GTCTTCATTTGATTTGGCAA+AGG 0.517828 4_3:-50275226 None:intergenic
CTTAGTGGAAGTGGTGGTAG+TGG 0.518560 4_3:+50274920 Msa0645320:CDS
GATTGAATATAAAATAGCAT+TGG 0.523394 4_3:-50274047 None:intergenic
AGGTTTATGAATAGTCTTAG+TGG 0.525192 4_3:+50274905 Msa0645320:CDS
TCTCGTGCCAAACATGGATA+AGG 0.525796 4_3:+50277471 Msa0645320:CDS
CACATCAGACATGTTGGACT+TGG 0.527533 4_3:+50277598 Msa0645320:CDS
GATGTAAAACCATTTCCTCC+AGG 0.529045 4_3:+50276474 Msa0645320:CDS
AGACATGGCAGTTCACCTGC+TGG 0.529643 4_3:+50275268 Msa0645320:CDS
TACAAAGTATATACTCCCTC+TGG 0.535243 4_3:-50277919 None:intergenic
ACCCAAGAAGCATTGCAGAA+AGG 0.537449 4_3:+50276950 Msa0645320:CDS
TGCAAAATTCGTGCTAAACG+TGG 0.539427 4_3:+50276916 Msa0645320:CDS
TCCTCCCATGTGGTACCTAA+GGG 0.540312 4_3:-50276396 None:intergenic
GCTTCCTATAGTTAAGGAAG+AGG 0.542479 4_3:+50275057 Msa0645320:CDS
CGAGCGAATGAGGAAACTAC+AGG 0.543755 4_3:+50277447 Msa0645320:CDS
AACAAACACATCAGACATGT+TGG 0.549768 4_3:+50277592 Msa0645320:CDS
ATGAGAGGAATAGGAACTAT+GGG 0.549779 4_3:+50276195 Msa0645320:CDS
TGTTTGTACATACCTCAACT+TGG 0.553379 4_3:-50277649 None:intergenic
ATGTTTGAAAACAATCCAGC+AGG 0.553542 4_3:-50275283 None:intergenic
CTCCCGATTTCAGCTCCTAA+CGG 0.555564 4_3:-50276297 None:intergenic
ATGGTTGTTTGGGAATGGTG+GGG 0.558888 4_3:+50278138 Msa0645320:three_prime_UTR
CAAAACAATCTTGAACCTGA+AGG 0.566426 4_3:+50276333 Msa0645320:CDS
GTGGTACCTAAGGGGAAACC+CGG 0.567524 4_3:-50276387 None:intergenic
CGGTCTAGTCTTATTAGACA+TGG 0.571581 4_3:+50275253 Msa0645320:CDS
ACAGGATCTCGTGCCAAACA+TGG 0.576148 4_3:+50277465 Msa0645320:CDS
GTTTCCCCTTAGGTACCACA+TGG 0.584871 4_3:+50276391 Msa0645320:CDS
AAGTTGTTTATAAGTATGAT+CGG 0.586683 4_3:-50277788 None:intergenic
GTGAAGAAACCTCTATAGGA+AGG 0.588934 4_3:-50274085 None:intergenic
AAATGAATTCAGGGTTAACA+CGG 0.591208 4_3:+50274777 Msa0645320:CDS
AAGAGAGAAAGAAAGAACAA+AGG 0.593891 4_3:-50273870 None:intergenic
AATGAATAACAATGAATCTG+TGG 0.596436 4_3:+50275108 Msa0645320:CDS
TTGCCAAATCAAATGAAGAC+CGG 0.596515 4_3:+50275229 Msa0645320:CDS
TGTTTATAAATGTGACCAGA+GGG 0.604137 4_3:+50277904 Msa0645320:three_prime_UTR
GATGAGTATGCCAAATACTA+CGG 0.608721 4_3:+50276843 Msa0645320:CDS
CCCCTTAGGTACCACATGGG+AGG 0.612122 4_3:+50276395 Msa0645320:CDS
ATACTCATCTGATGAGCCAA+AGG 0.612846 4_3:-50276829 None:intergenic
TCTAATAAGACTAGACCGAC+CGG 0.616887 4_3:-50275248 None:intergenic
AATAGTCTTAGTGGAAGTGG+TGG 0.627049 4_3:+50274914 Msa0645320:CDS
CAAATCAAATGAAGACCGGT+CGG 0.636942 4_3:+50275233 Msa0645320:CDS
TTGTTTATAAATGTGACCAG+AGG 0.655995 4_3:+50277903 Msa0645320:three_prime_UTR
CCTCCCATGTGGTACCTAAG+GGG 0.663126 4_3:-50276395 None:intergenic
TGGTACCTAAGGGGAAACCC+GGG 0.679069 4_3:-50276386 None:intergenic
GCGTGTAGTGAAGAGAGTGG+TGG 0.682382 4_3:-50274121 None:intergenic
AGACCTACAAAACCAAGTTG+AGG 0.687966 4_3:+50277637 Msa0645320:CDS
TTGTTTATAAGTATGATCGG+AGG 0.700713 4_3:-50277785 None:intergenic
TTTCCCCTTAGGTACCACAT+GGG 0.701805 4_3:+50276392 Msa0645320:CDS
GTAGCGTGTAGTGAAGAGAG+TGG 0.703050 4_3:-50274124 None:intergenic
GATTTCAGCTCCTAACGGTG+AGG 0.706801 4_3:-50276292 None:intergenic
CATCGCAGTATCCTCCCATG+TGG 0.735897 4_3:-50276406 None:intergenic
AACCAAATATGAATGGAACG+TGG 0.761445 4_3:-50273910 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGAAAAATATGAACT+TGG - chr4_3:50274815-50274834 None:intergenic 15.0%
!! TAATGATGTATGAATAATTA+TGG - chr4_3:50277723-50277742 None:intergenic 15.0%
!! GATTGAATATAAAATAGCAT+TGG - chr4_3:50274050-50274069 None:intergenic 20.0%
!! ATAGTTCATATCTTTGTATA+TGG + chr4_3:50274248-50274267 Msa0645320:intron 20.0%
!! TGATAAAAAATTGTTACACT+TGG + chr4_3:50274316-50274335 Msa0645320:intron 20.0%
!! AAAAACTGAAAAAGATGAAA+GGG - chr4_3:50274506-50274525 None:intergenic 20.0%
!! AAGACAACAAAGTAATAATT+TGG + chr4_3:50275147-50275166 Msa0645320:CDS 20.0%
!! AGACAACAAAGTAATAATTT+GGG + chr4_3:50275148-50275167 Msa0645320:CDS 20.0%
!! AGTAATAATTTGGGTAATTA+TGG + chr4_3:50275157-50275176 Msa0645320:CDS 20.0%
!!! TGTAGTTTTTATCAAAATCA+CGG + chr4_3:50275573-50275592 Msa0645320:intron 20.0%
!!! ATTTACATTTTGTGCATATT+TGG - chr4_3:50275634-50275653 None:intergenic 20.0%
!!! ATTTACATTTTGTGCATATT+TGG - chr4_3:50275634-50275653 None:intergenic 20.0%
!! AAGTTAATTAGAAATCCAAT+AGG + chr4_3:50276084-50276103 Msa0645320:intron 20.0%
!!! TCATTTTCTTGATCATAAAT+AGG + chr4_3:50276127-50276146 Msa0645320:intron 20.0%
!! GTACAAGTGAAAAATAAAAT+GGG - chr4_3:50277005-50277024 None:intergenic 20.0%
!! TGTACAAGTGAAAAATAAAA+TGG - chr4_3:50277006-50277025 None:intergenic 20.0%
!! TTTACTAGTTGACAAATAAT+TGG + chr4_3:50277184-50277203 Msa0645320:intron 20.0%
!!! TTATTCAATGGTTACTTTAT+CGG + chr4_3:50277225-50277244 Msa0645320:intron 20.0%
!! ATAATTCAAAGTAGTTATGT+AGG - chr4_3:50277528-50277547 None:intergenic 20.0%
!! TACATAACTACTTTGAATTA+TGG + chr4_3:50277527-50277546 Msa0645320:intron 20.0%
!! AAGTTGTTTATAAGTATGAT+CGG - chr4_3:50277791-50277810 None:intergenic 20.0%
!! ATTAAATAAGTGATGTATCT+AGG - chr4_3:50277827-50277846 None:intergenic 20.0%
!!! CAGATTTTGTTGTAATAATA+TGG + chr4_3:50278052-50278071 Msa0645320:three_prime_UTR 20.0%
! TTGAGAAAACCAAATATGAA+TGG - chr4_3:50273920-50273939 None:intergenic 25.0%
!!! ATTCATATTTGGTTTTCTCA+AGG + chr4_3:50273919-50273938 Msa0645320:five_prime_UTR 25.0%
!!! TTCATATTTGGTTTTCTCAA+GGG + chr4_3:50273920-50273939 Msa0645320:five_prime_UTR 25.0%
! AAACAACAAAGACAATAGTT+TGG - chr4_3:50274348-50274367 None:intergenic 25.0%
!!! ACTATTGTCTTTGTTGTTTT+TGG + chr4_3:50274349-50274368 Msa0645320:intron 25.0%
! AAAACTGAAAAAGATGAAAG+GGG - chr4_3:50274505-50274524 None:intergenic 25.0%
! CAAAAACTGAAAAAGATGAA+AGG - chr4_3:50274507-50274526 None:intergenic 25.0%
!! GTGAAATAAATTGATTTCCT+TGG + chr4_3:50274537-50274556 Msa0645320:intron 25.0%
!! AAATAAATTGATTTCCTTGG+TGG + chr4_3:50274540-50274559 Msa0645320:intron 25.0%
!! AATAAATTGATTTCCTTGGT+GGG + chr4_3:50274541-50274560 Msa0645320:intron 25.0%
! AATGAATAACAATGAATCTG+TGG + chr4_3:50275108-50275127 Msa0645320:CDS 25.0%
! TCAAACATTAACATTGATAC+TGG + chr4_3:50275298-50275317 Msa0645320:CDS 25.0%
! TCATGATAAGAATAAGAGTA+TGG - chr4_3:50275331-50275350 None:intergenic 25.0%
!!! ATGGAAATAGGTATTGAAAA+GGG - chr4_3:50275394-50275413 None:intergenic 25.0%
!!! AATGGAAATAGGTATTGAAA+AGG - chr4_3:50275395-50275414 None:intergenic 25.0%
! CCATGTAAAACAAAGAAAAA+GGG - chr4_3:50275436-50275455 None:intergenic 25.0%
! ACCATGTAAAACAAAGAAAA+AGG - chr4_3:50275437-50275456 None:intergenic 25.0%
!!! GTGACTTTTTTAAATAACTC+CGG + chr4_3:50275506-50275525 Msa0645320:intron 25.0%
!! CTTACTAAAATTATGATGGA+TGG - chr4_3:50275676-50275695 None:intergenic 25.0%
!!! CATCCATCATAATTTTAGTA+AGG + chr4_3:50275674-50275693 Msa0645320:intron 25.0%
! GTTCCTTACTAAAATTATGA+TGG - chr4_3:50275680-50275699 None:intergenic 25.0%
!! CTTACTAAAATTATGATGGA+TGG - chr4_3:50275676-50275695 None:intergenic 25.0%
!!! CATCCATCATAATTTTAGTA+AGG + chr4_3:50275674-50275693 Msa0645320:intron 25.0%
! GTTCCTTACTAAAATTATGA+TGG - chr4_3:50275680-50275699 None:intergenic 25.0%
! GCAATAAATAGTAAACCTAT+TGG - chr4_3:50276102-50276121 None:intergenic 25.0%
! ATGATATCTGACAATATTAC+TGG + chr4_3:50276426-50276445 Msa0645320:CDS 25.0%
! TTGTTAAATAGAAGCTATAG+CGG + chr4_3:50276642-50276661 Msa0645320:intron 25.0%
!! TGCATAACAAAATGAGTTTT+TGG - chr4_3:50277095-50277114 None:intergenic 25.0%
! TAACTGTTGAAACTGTTTAA+AGG - chr4_3:50277317-50277336 None:intergenic 25.0%
!!! AAACAATGTTTGGTTTGTTA+AGG - chr4_3:50277556-50277575 None:intergenic 25.0%
! AAAAAGTGAGAAACAATGTT+TGG - chr4_3:50277566-50277585 None:intergenic 25.0%
! CAATAAGGGAAAAGAATTAA+AGG + chr4_3:50277997-50278016 Msa0645320:three_prime_UTR 25.0%
AAGAGAGAAAGAAAGAACAA+AGG - chr4_3:50273873-50273892 None:intergenic 30.0%
!!! ACTTTTTTGTTGTTGTGTTG+GGG - chr4_3:50274163-50274182 None:intergenic 30.0%
!!! CACTTTTTTGTTGTTGTGTT+GGG - chr4_3:50274164-50274183 None:intergenic 30.0%
!!! ACACTTTTTTGTTGTTGTGT+TGG - chr4_3:50274165-50274184 None:intergenic 30.0%
!!! GCACTAGAGGTATTTTTTTT+TGG + chr4_3:50274186-50274205 Msa0645320:intron 30.0%
TGTTTAACTATAAGAGACAC+TGG + chr4_3:50274275-50274294 Msa0645320:intron 30.0%
AAACTGAAAAAGATGAAAGG+GGG - chr4_3:50274504-50274523 None:intergenic 30.0%
GAAGAAGAAGAAGAAAAGAA+TGG + chr4_3:50274694-50274713 Msa0645320:exon 30.0%
TGATGATCTAGAAACATAGT+TGG - chr4_3:50274740-50274759 None:intergenic 30.0%
ATCAGCAACAAATGAATTCA+GGG + chr4_3:50274768-50274787 Msa0645320:CDS 30.0%
AAATGAATTCAGGGTTAACA+CGG + chr4_3:50274777-50274796 Msa0645320:CDS 30.0%
! AAACAGAAAGAGTTTTTGCA+AGG + chr4_3:50274885-50274904 Msa0645320:CDS 30.0%
! AGGTTTATGAATAGTCTTAG+TGG + chr4_3:50274905-50274924 Msa0645320:CDS 30.0%
TCAACTACTGAATCAGTTAT+AGG - chr4_3:50274998-50275017 None:intergenic 30.0%
GAATTATGGTAAAAACTCTG+AGG - chr4_3:50275196-50275215 None:intergenic 30.0%
! CAGAGTTTTTACCATAATTC+AGG + chr4_3:50275196-50275215 Msa0645320:CDS 30.0%
AAAAAAGAGGCAATGGAAAT+AGG - chr4_3:50275406-50275425 None:intergenic 30.0%
!!! CCCTTTTTCTTTGTTTTACA+TGG + chr4_3:50275433-50275452 Msa0645320:intron 30.0%
! TTTTAAATAACTCCGGAATC+CGG + chr4_3:50275513-50275532 Msa0645320:intron 30.0%
! AGTTTTTATCAAAATCACGG+TGG + chr4_3:50275576-50275595 Msa0645320:intron 30.0%
! CATTTTGTGCATATTTGGAT+CGG - chr4_3:50275629-50275648 None:intergenic 30.0%
AGAGGACTTGATTTAAATCT+AGG + chr4_3:50275985-50276004 Msa0645320:intron 30.0%
! AGCAAATTTTTCTTCTTCTG+CGG + chr4_3:50276242-50276261 Msa0645320:CDS 30.0%
! CATCATCTCTGTATCTTTTT+AGG - chr4_3:50276452-50276471 None:intergenic 30.0%
TTTAAATCCAGCTGAAACTA+AGG + chr4_3:50276497-50276516 Msa0645320:intron 30.0%
TGAATTGTACGATTGGAATT+TGG + chr4_3:50276524-50276543 Msa0645320:intron 30.0%
TTGAATAAGAATCTCTGTTG+TGG - chr4_3:50277213-50277232 None:intergenic 30.0%
CAACAGAGATTCTTATTCAA+TGG + chr4_3:50277213-50277232 Msa0645320:intron 30.0%
TATTATCGAGTTCTACAACA+AGG - chr4_3:50277260-50277279 None:intergenic 30.0%
CTGAAAATCAATTCACATGA+TGG - chr4_3:50277407-50277426 None:intergenic 30.0%
!! CATCATGTGAATTGATTTTC+AGG + chr4_3:50277405-50277424 Msa0645320:intron 30.0%
ATCTTTACCTTATCCATGTT+TGG - chr4_3:50277481-50277500 None:intergenic 30.0%
!!! AAGGTAAAGATTTTGACTTG+TGG + chr4_3:50277490-50277509 Msa0645320:intron 30.0%
TTCAAAGTAGTTATGTAGGT+TGG - chr4_3:50277524-50277543 None:intergenic 30.0%
TTGTTTATAAGTATGATCGG+AGG - chr4_3:50277788-50277807 None:intergenic 30.0%
TTGTTTATAAATGTGACCAG+AGG + chr4_3:50277903-50277922 Msa0645320:three_prime_UTR 30.0%
TGTTTATAAATGTGACCAGA+GGG + chr4_3:50277904-50277923 Msa0645320:three_prime_UTR 30.0%
!!! TGATGTTGTCACTTTTTCTA+TGG + chr4_3:50278119-50278138 Msa0645320:three_prime_UTR 30.0%
!!! TCACTTTTTCTATGGTTGTT+TGG + chr4_3:50278127-50278146 Msa0645320:three_prime_UTR 30.0%
!!! CACTTTTTCTATGGTTGTTT+GGG + chr4_3:50278128-50278147 Msa0645320:three_prime_UTR 30.0%
AACCAAATATGAATGGAACG+TGG - chr4_3:50273913-50273932 None:intergenic 35.0%
GAAAGTGAAGAAACCTCTAT+AGG - chr4_3:50274092-50274111 None:intergenic 35.0%
!!! CTTTTTTGTTGTTGTGTTGG+GGG - chr4_3:50274162-50274181 None:intergenic 35.0%
!! ACAAAAAAGTGTTGCACTAG+AGG + chr4_3:50274173-50274192 Msa0645320:five_prime_UTR 35.0%
TCACTCAGCATCAAAACAAA+CGG - chr4_3:50274403-50274422 None:intergenic 35.0%
ATAACAAAGAAAACCCACCA+AGG - chr4_3:50274557-50274576 None:intergenic 35.0%
CATCAGCAACAAATGAATTC+AGG + chr4_3:50274767-50274786 Msa0645320:CDS 35.0%
GCAAAAACTCTTTCTGTTTC+AGG - chr4_3:50274884-50274903 None:intergenic 35.0%
! ATGAATAGTCTTAGTGGAAG+TGG + chr4_3:50274911-50274930 Msa0645320:CDS 35.0%
TCAACCTCTTCCTTAACTAT+AGG - chr4_3:50275064-50275083 None:intergenic 35.0%
!! TTCATTTGATTTGGCAAAGG+TGG - chr4_3:50275226-50275245 None:intergenic 35.0%
!! GTCTTCATTTGATTTGGCAA+AGG - chr4_3:50275229-50275248 None:intergenic 35.0%
TTGCCAAATCAAATGAAGAC+CGG + chr4_3:50275229-50275248 Msa0645320:CDS 35.0%
ATGTTTGAAAACAATCCAGC+AGG - chr4_3:50275286-50275305 None:intergenic 35.0%
AATCACGAAAAAAGAGGCAA+TGG - chr4_3:50275413-50275432 None:intergenic 35.0%
AAAGGGAATCACGAAAAAAG+AGG - chr4_3:50275419-50275438 None:intergenic 35.0%
ATCATCGTGTCAATCTAATC+CGG - chr4_3:50275535-50275554 None:intergenic 35.0%
! AGATTTTGCTGAATTCGTTG+TGG + chr4_3:50275606-50275625 Msa0645320:intron 35.0%
! CATTTTCCAGCTAATTGGAT+CGG - chr4_3:50275891-50275910 None:intergenic 35.0%
! GTTCTCATTTTCCAGCTAAT+TGG - chr4_3:50275896-50275915 None:intergenic 35.0%
GCTGGAAAATGAGAACATAA+TGG + chr4_3:50275900-50275919 Msa0645320:intron 35.0%
! TCATGAAAGTACAACTCAGT+TGG + chr4_3:50275939-50275958 Msa0645320:intron 35.0%
GGCTACTTGACCTAAAAAAA+CGG + chr4_3:50276036-50276055 Msa0645320:intron 35.0%
GCTACTTGACCTAAAAAAAC+GGG + chr4_3:50276037-50276056 Msa0645320:intron 35.0%
!!! TCACGAAATCCCGTTTTTTT+AGG - chr4_3:50276049-50276068 None:intergenic 35.0%
TATAATGATGCTAGCTAGCT+TGG + chr4_3:50276154-50276173 Msa0645320:intron 35.0%
TATGAGAGGAATAGGAACTA+TGG + chr4_3:50276194-50276213 Msa0645320:CDS 35.0%
ATGAGAGGAATAGGAACTAT+GGG + chr4_3:50276195-50276214 Msa0645320:CDS 35.0%
! ATTTTTCTTCTTCTGCGGTA+CGG + chr4_3:50276247-50276266 Msa0645320:CDS 35.0%
!! TCAGGTTCAAGATTGTTTTG+TGG - chr4_3:50276333-50276352 None:intergenic 35.0%
CAAAACAATCTTGAACCTGA+AGG + chr4_3:50276333-50276352 Msa0645320:CDS 35.0%
AGGTAGTTGAATTGTACGAT+TGG + chr4_3:50276517-50276536 Msa0645320:intron 35.0%
ATAGCTATTGTTCTGCAATG+CGG + chr4_3:50276603-50276622 Msa0645320:intron 35.0%
GATGAGTATGCCAAATACTA+CGG + chr4_3:50276843-50276862 Msa0645320:CDS 35.0%
GAACTGCAAAAACTTCTCAA+TGG - chr4_3:50276881-50276900 None:intergenic 35.0%
! CTCAGTCACTTATTCAAGTT+TGG + chr4_3:50277062-50277081 Msa0645320:intron 35.0%
TGTGCAAGATTGTAAAGATG+TGG - chr4_3:50277159-50277178 None:intergenic 35.0%
! AATCAATTCACATGATGGTG+AGG - chr4_3:50277402-50277421 None:intergenic 35.0%
AACAAACACATCAGACATGT+TGG + chr4_3:50277592-50277611 Msa0645320:CDS 35.0%
!!! ATACCTCAACTTGGTTTTGT+AGG - chr4_3:50277643-50277662 None:intergenic 35.0%
TGTTTGTACATACCTCAACT+TGG - chr4_3:50277652-50277671 None:intergenic 35.0%
TAGAGAAGTGAGAATGAGAT+TGG - chr4_3:50277680-50277699 None:intergenic 35.0%
!! CGGAGGAAGTATTTATAGAA+AGG - chr4_3:50277771-50277790 None:intergenic 35.0%
TACAAAGTATATACTCCCTC+TGG - chr4_3:50277922-50277941 None:intergenic 35.0%
TTTCTATGGTTGTTTGGGAA+TGG + chr4_3:50278133-50278152 Msa0645320:three_prime_UTR 35.0%
TGCCACGTTCCATTCATATT+TGG + chr4_3:50273908-50273927 Msa0645320:five_prime_UTR 40.0%
CACACAACAAATCTGAAAGC+TGG + chr4_3:50273973-50273992 Msa0645320:five_prime_UTR 40.0%
TCACTCTGTTCACTCCTTAT+TGG + chr4_3:50274001-50274020 Msa0645320:five_prime_UTR 40.0%
! ATTGGATTTTGTTGCTGTGG+AGG - chr4_3:50274032-50274051 None:intergenic 40.0%
!! AGCATTGGATTTTGTTGCTG+TGG - chr4_3:50274035-50274054 None:intergenic 40.0%
AGGAAGGTTCAAGAGAGAAA+AGG - chr4_3:50274072-50274091 None:intergenic 40.0%
TCTCTTGAACCTTCCTATAG+AGG + chr4_3:50274076-50274095 Msa0645320:five_prime_UTR 40.0%
GTGAAGAAACCTCTATAGGA+AGG - chr4_3:50274088-50274107 None:intergenic 40.0%
CTATAAGAGACACTGGTAAC+AGG + chr4_3:50274282-50274301 Msa0645320:intron 40.0%
GATGCTGAGTGATAGAAACT+TGG + chr4_3:50274411-50274430 Msa0645320:intron 40.0%
CTTTCTGTTTCAGGACTTGA+AGG - chr4_3:50274875-50274894 None:intergenic 40.0%
! AATAGTCTTAGTGGAAGTGG+TGG + chr4_3:50274914-50274933 Msa0645320:CDS 40.0%
GCAACAGCTTCCTATAGTTA+AGG + chr4_3:50275051-50275070 Msa0645320:CDS 40.0%
GCTTCCTATAGTTAAGGAAG+AGG + chr4_3:50275057-50275076 Msa0645320:CDS 40.0%
AAGGTGGTCTTCCTGAATTA+TGG - chr4_3:50275210-50275229 None:intergenic 40.0%
CAAATCAAATGAAGACCGGT+CGG + chr4_3:50275233-50275252 Msa0645320:CDS 40.0%
TCTAATAAGACTAGACCGAC+CGG - chr4_3:50275251-50275270 None:intergenic 40.0%
!! CGGTCTAGTCTTATTAGACA+TGG + chr4_3:50275253-50275272 Msa0645320:CDS 40.0%
TGTCAATCTAATCCGGATTC+CGG - chr4_3:50275528-50275547 None:intergenic 40.0%
! GAAAGTACAACTCAGTTGGT+AGG + chr4_3:50275943-50275962 Msa0645320:intron 40.0%
!! TTTTAGGTCAAGTAGCCTAG+TGG - chr4_3:50276033-50276052 None:intergenic 40.0%
TTTGCTGCTATGAGAGGAAT+AGG + chr4_3:50276186-50276205 Msa0645320:CDS 40.0%
! CTGAAGGTTTTGCTGAAACT+CGG + chr4_3:50276349-50276368 Msa0645320:CDS 40.0%
! TGAAGGTTTTGCTGAAACTC+GGG + chr4_3:50276350-50276369 Msa0645320:CDS 40.0%
GATGTAAAACCATTTCCTCC+AGG + chr4_3:50276474-50276493 Msa0645320:CDS 40.0%
GGATTTAAACCTGGAGGAAA+TGG - chr4_3:50276486-50276505 None:intergenic 40.0%
GTTTCAGCTGGATTTAAACC+TGG - chr4_3:50276495-50276514 None:intergenic 40.0%
TCAACTACCTTAGTTTCAGC+TGG - chr4_3:50276507-50276526 None:intergenic 40.0%
! TTGCCAACACTGATTACATC+TGG + chr4_3:50276732-50276751 Msa0645320:intron 40.0%
ACACCAGATGTAATCAGTGT+TGG - chr4_3:50276738-50276757 None:intergenic 40.0%
! TGCATCGATCAGAATGAAAC+AGG + chr4_3:50276787-50276806 Msa0645320:intron 40.0%
ATCGATCAGAATGAAACAGG+AGG + chr4_3:50276790-50276809 Msa0645320:intron 40.0%
ATACTCATCTGATGAGCCAA+AGG - chr4_3:50276832-50276851 None:intergenic 40.0%
! CGTTTAGCACGAATTTTGCA+CGG - chr4_3:50276916-50276935 None:intergenic 40.0%
TGCAAAATTCGTGCTAAACG+TGG + chr4_3:50276916-50276935 Msa0645320:CDS 40.0%
GAGTTCTACAACAAGGAATC+TGG - chr4_3:50277253-50277272 None:intergenic 40.0%
! TGATGGTGAGGAAACATATG+TGG - chr4_3:50277390-50277409 None:intergenic 40.0%
CTAAAATTAGCGAGCGAATG+AGG + chr4_3:50277437-50277456 Msa0645320:CDS 40.0%
AGACCTACAAAACCAAGTTG+AGG + chr4_3:50277637-50277656 Msa0645320:CDS 40.0%
TCACACAAGCAACAGCAATA+AGG + chr4_3:50277982-50278001 Msa0645320:three_prime_UTR 40.0%
CACACAAGCAACAGCAATAA+GGG + chr4_3:50277983-50278002 Msa0645320:three_prime_UTR 40.0%
TATGGTTGTTTGGGAATGGT+GGG + chr4_3:50278137-50278156 Msa0645320:three_prime_UTR 40.0%
CGACCGGTCTTCATTTGATT+TGG - chr4_3:50275235-50275254 None:intergenic 45.0%
ATTTGGATCGGCAGTCAGTT+CGG - chr4_3:50275748-50275767 None:intergenic 45.0%
AATTGGATCGGCAGTCAGTT+CGG - chr4_3:50275879-50275898 None:intergenic 45.0%
GCAGGGACATTGATATACAG+AGG + chr4_3:50275967-50275986 Msa0645320:intron 45.0%
! ATGATGCTAGCTAGCTTGGT+TGG + chr4_3:50276158-50276177 Msa0645320:intron 45.0%
TCACCGTTAGGAGCTGAAAT+CGG + chr4_3:50276294-50276313 Msa0645320:CDS 45.0%
CGAGTTTCAGCAAAACCTTC+AGG - chr4_3:50276351-50276370 None:intergenic 45.0%
! GAAGGTTTTGCTGAAACTCG+GGG + chr4_3:50276351-50276370 Msa0645320:CDS 45.0%
! TCGGGGTAACGATTTTATCC+CGG + chr4_3:50276368-50276387 Msa0645320:CDS 45.0%
TTTCCCCTTAGGTACCACAT+GGG + chr4_3:50276392-50276411 Msa0645320:CDS 45.0%
TCAGCTGGATTTAAACCTGG+AGG - chr4_3:50276492-50276511 None:intergenic 45.0%
CTTCTCAATGGCTGCCAATT+CGG - chr4_3:50276869-50276888 None:intergenic 45.0%
!! ACCCAAGAAGCATTGCAGAA+AGG + chr4_3:50276950-50276969 Msa0645320:CDS 45.0%
CCCTTTCTGCAATGCTTCTT+GGG - chr4_3:50276954-50276973 None:intergenic 45.0%
!! CCCAAGAAGCATTGCAGAAA+GGG + chr4_3:50276951-50276970 Msa0645320:CDS 45.0%
ACCCTTTCTGCAATGCTTCT+TGG - chr4_3:50276955-50276974 None:intergenic 45.0%
TCTCGTGCCAAACATGGATA+AGG + chr4_3:50277471-50277490 Msa0645320:CDS 45.0%
CACATCAGACATGTTGGACT+TGG + chr4_3:50277598-50277617 Msa0645320:CDS 45.0%
CTATGGTTGTTTGGGAATGG+TGG + chr4_3:50278136-50278155 Msa0645320:three_prime_UTR 45.0%
ATGGTTGTTTGGGAATGGTG+GGG + chr4_3:50278138-50278157 Msa0645320:three_prime_UTR 45.0%
GTAGCGTGTAGTGAAGAGAG+TGG - chr4_3:50274127-50274146 None:intergenic 50.0%
! TTGTTGTTGTGTTGGGGGTG+TGG - chr4_3:50274157-50274176 None:intergenic 50.0%
CTTAGTGGAAGTGGTGGTAG+TGG + chr4_3:50274920-50274939 Msa0645320:CDS 50.0%
TGACTGCCGATCCAATTAGC+TGG + chr4_3:50275882-50275901 Msa0645320:intron 50.0%
!! ACAACTCAGTTGGTAGGTGC+AGG + chr4_3:50275949-50275968 Msa0645320:intron 50.0%
!! CAACTCAGTTGGTAGGTGCA+GGG + chr4_3:50275950-50275969 Msa0645320:intron 50.0%
! GCAGGTTTTGCTGCTATGAG+AGG + chr4_3:50276180-50276199 Msa0645320:intron 50.0%
CCTAACGGTGAGGCATAGTT+TGG - chr4_3:50276285-50276304 None:intergenic 50.0%
CCAAACTATGCCTCACCGTT+AGG + chr4_3:50276282-50276301 Msa0645320:CDS 50.0%
GATTTCAGCTCCTAACGGTG+AGG - chr4_3:50276295-50276314 None:intergenic 50.0%
CACCGTTAGGAGCTGAAATC+GGG + chr4_3:50276295-50276314 Msa0645320:CDS 50.0%
CTCCCGATTTCAGCTCCTAA+CGG - chr4_3:50276300-50276319 None:intergenic 50.0%
! CGGGGTAACGATTTTATCCC+GGG + chr4_3:50276369-50276388 Msa0645320:CDS 50.0%
TTTATCCCGGGTTTCCCCTT+AGG + chr4_3:50276381-50276400 Msa0645320:CDS 50.0%
GTTTCCCCTTAGGTACCACA+TGG + chr4_3:50276391-50276410 Msa0645320:CDS 50.0%
TCCTCCCATGTGGTACCTAA+GGG - chr4_3:50276399-50276418 None:intergenic 50.0%
ATCCTCCCATGTGGTACCTA+AGG - chr4_3:50276400-50276419 None:intergenic 50.0%
CCAAAGGAGCAGAAGTAGTC+TGG - chr4_3:50276816-50276835 None:intergenic 50.0%
CCAGACTACTTCTGCTCCTT+TGG + chr4_3:50276813-50276832 Msa0645320:CDS 50.0%
GCCAAATACTACGGCCGAAT+TGG + chr4_3:50276852-50276871 Msa0645320:CDS 50.0%
GCCAATTCGGCCGTAGTATT+TGG - chr4_3:50276856-50276875 None:intergenic 50.0%
TGCAATGCTTCTTGGGTGAG+TGG - chr4_3:50276947-50276966 None:intergenic 50.0%
CGAGCGAATGAGGAAACTAC+AGG + chr4_3:50277447-50277466 Msa0645320:CDS 50.0%
ACAGGATCTCGTGCCAAACA+TGG + chr4_3:50277465-50277484 Msa0645320:CDS 50.0%
! CTGTGGAGGTGCAGCCAATA+AGG - chr4_3:50274018-50274037 None:intergenic 55.0%
GCGTGTAGTGAAGAGAGTGG+TGG - chr4_3:50274124-50274143 None:intergenic 55.0%
!! AGTGGAAGTGGTGGTAGTGG+TGG + chr4_3:50274923-50274942 Msa0645320:CDS 55.0%
AGACATGGCAGTTCACCTGC+TGG + chr4_3:50275268-50275287 Msa0645320:CDS 55.0%
AGGTCAAGTAGCCTAGTGGC+AGG - chr4_3:50276029-50276048 None:intergenic 55.0%
! TGCTAGCTAGCTTGGTTGGC+AGG + chr4_3:50276162-50276181 Msa0645320:intron 55.0%
TGGTACCTAAGGGGAAACCC+GGG - chr4_3:50276389-50276408 None:intergenic 55.0%
GTGGTACCTAAGGGGAAACC+CGG - chr4_3:50276390-50276409 None:intergenic 55.0%
CCTCCCATGTGGTACCTAAG+GGG - chr4_3:50276398-50276417 None:intergenic 55.0%
CATCGCAGTATCCTCCCATG+TGG - chr4_3:50276409-50276428 None:intergenic 55.0%
!! GGAAGTGGTGGTAGTGGTGG+TGG + chr4_3:50274926-50274945 Msa0645320:CDS 60.0%
!! AGTGGTGGTAGTGGTGGTGG+TGG + chr4_3:50274929-50274948 Msa0645320:CDS 60.0%
TAGTGGCAGGCGTGGAGAAG+TGG - chr4_3:50276016-50276035 None:intergenic 60.0%
ACTTCTCCACGCCTGCCACT+AGG + chr4_3:50276015-50276034 Msa0645320:intron 60.0%
AAGTAGCCTAGTGGCAGGCG+TGG - chr4_3:50276024-50276043 None:intergenic 60.0%
CCCCTTAGGTACCACATGGG+AGG + chr4_3:50276395-50276414 Msa0645320:CDS 60.0%
!! GGTGGTAGTGGTGGTGGTGG+TGG + chr4_3:50274932-50274951 Msa0645320:CDS 65.0%
!! GGTAGTGGTGGTGGTGGTGG+TGG + chr4_3:50274935-50274954 Msa0645320:CDS 65.0%
!! AGTGGTGGTGGTGGTGGTGG+TGG + chr4_3:50274938-50274957 Msa0645320:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 50273862 50278264 50273862 ID=Msa0645320;Name=Msa0645320
chr4_3 mRNA 50273862 50278264 50273862 ID=Msa0645320-mRNA-1;Parent=Msa0645320;Name=Msa0645320-mRNA-1;_AED=0.06;_eAED=0.06;_QI=399|0.83|0.71|1|0.66|0.57|7|511|411
chr4_3 exon 50273862 50274194 50273862 ID=Msa0645320-mRNA-1:exon:11970;Parent=Msa0645320-mRNA-1
chr4_3 exon 50274647 50275319 50274647 ID=Msa0645320-mRNA-1:exon:11971;Parent=Msa0645320-mRNA-1
chr4_3 exon 50276184 50276495 50276184 ID=Msa0645320-mRNA-1:exon:11972;Parent=Msa0645320-mRNA-1
chr4_3 exon 50276809 50276972 50276809 ID=Msa0645320-mRNA-1:exon:11973;Parent=Msa0645320-mRNA-1
chr4_3 exon 50277427 50277492 50277427 ID=Msa0645320-mRNA-1:exon:11974;Parent=Msa0645320-mRNA-1
chr4_3 exon 50277590 50277797 50277590 ID=Msa0645320-mRNA-1:exon:11975;Parent=Msa0645320-mRNA-1
chr4_3 exon 50277875 50278264 50277875 ID=Msa0645320-mRNA-1:exon:11976;Parent=Msa0645320-mRNA-1
chr4_3 five_prime_UTR 50273862 50274194 50273862 ID=Msa0645320-mRNA-1:five_prime_utr;Parent=Msa0645320-mRNA-1
chr4_3 five_prime_UTR 50274647 50274712 50274647 ID=Msa0645320-mRNA-1:five_prime_utr;Parent=Msa0645320-mRNA-1
chr4_3 CDS 50274713 50275319 50274713 ID=Msa0645320-mRNA-1:cds;Parent=Msa0645320-mRNA-1
chr4_3 CDS 50276184 50276495 50276184 ID=Msa0645320-mRNA-1:cds;Parent=Msa0645320-mRNA-1
chr4_3 CDS 50276809 50276972 50276809 ID=Msa0645320-mRNA-1:cds;Parent=Msa0645320-mRNA-1
chr4_3 CDS 50277427 50277492 50277427 ID=Msa0645320-mRNA-1:cds;Parent=Msa0645320-mRNA-1
chr4_3 CDS 50277590 50277676 50277590 ID=Msa0645320-mRNA-1:cds;Parent=Msa0645320-mRNA-1
chr4_3 three_prime_UTR 50277677 50277797 50277677 ID=Msa0645320-mRNA-1:three_prime_utr;Parent=Msa0645320-mRNA-1
chr4_3 three_prime_UTR 50277875 50278264 50277875 ID=Msa0645320-mRNA-1:three_prime_utr;Parent=Msa0645320-mRNA-1
Gene Sequence

>Msa0645320

ATGGAGTCAGATCTTCAGCAGCATCCAACTATGTTTCTAGATCATCAAAATCATCATCAGCAACAAATGAATTCAGGGTTAACACGGTTTAAATCTGCTCCAAGTTCATATTTTTCAAATATCATTGATAAAGAGTTTTATGAGCATCTTTTCAATAAACCTTCAAGTCCTGAAACAGAAAGAGTTTTTGCAAGGTTTATGAATAGTCTTAGTGGAAGTGGTGGTAGTGGTGGTGGTGGTGGTGGTGGTGCTGAAAGTGTTTCTGTTACAGCTTCTATAGCTCCTATAACTGATTCAGTAGTTGATTCATTGACTCAGAATCTATCAACTGTTCAACAGCAACAGCTTCCTATAGTTAAGGAAGAGGTTGATCAACAATCTCAAACTATGCAAACAATGAATAACAATGAATCTGTGGAACTTCAACAACTTCAAAGACAACAAAGTAATAATTTGGGTAATTATGGTTCTTCTGCTGCTCCTCAGAGTTTTTACCATAATTCAGGAAGACCACCTTTGCCAAATCAAATGAAGACCGGTCGGTCTAGTCTTATTAGACATGGCAGTTCACCTGCTGGATTGTTTTCAAACATTAACATTGATACTGGTTTTGCTGCTATGAGAGGAATAGGAACTATGGGAGCTGCTAATAGCGCTAGCAAAGAAGCAAATTTTTCTTCTTCTGCGGTACGGTTAAAGAATGCTCCAAACTATGCCTCACCGTTAGGAGCTGAAATCGGGAGTAATAGCATTCCACAAAACAATCTTGAACCTGAAGGTTTTGCTGAAACTCGGGGTAACGATTTTATCCCGGGTTTCCCCTTAGGTACCACATGGGAGGATACTGCGATGATATCTGACAATATTACTGGCCTAAAAAGATACAGAGATGATGATGATGTAAAACCATTTCCTCCAGGAGGCCAGACTACTTCTGCTCCTTTGGCTCATCAGATGAGTATGCCAAATACTACGGCCGAATTGGCAGCCATTGAGAAGTTTTTGCAGTTCTCAGATTCTGTTCCGTGCAAAATTCGTGCTAAACGTGGCTGTGCCACTCACCCAAGAAGCATTGCAGAAAGGGTTAGAAGAACTAAAATTAGCGAGCGAATGAGGAAACTACAGGATCTCGTGCCAAACATGGATAAGCAAACAAACACATCAGACATGTTGGACTTGGCTGTTGAGTACATTAAAGACCTACAAAACCAAGTTGAGGTATGTACAAACAATTGA
Protein sequence

>Msa0645320

MESDLQQHPTMFLDHQNHHQQQMNSGLTRFKSAPSSYFSNIIDKEFYEHLFNKPSSPETERVFARFMNSLSGSGGSGGGGGGGAESVSVTASIAPITDSVVDSLTQNLSTVQQQQLPIVKEEVDQQSQTMQTMNNNESVELQQLQRQQSNNLGNYGSSAAPQSFYHNSGRPPLPNQMKTGRSSLIRHGSSPAGLFSNINIDTGFAAMRGIGTMGAANSASKEANFSSSAVRLKNAPNYASPLGAEIGSNSIPQNNLEPEGFAETRGNDFIPGFPLGTTWEDTAMISDNITGLKRYRDDDDVKPFPPGGQTTSAPLAHQMSMPNTTAELAAIEKFLQFSDSVPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDKQTNTSDMLDLAVEYIKDLQNQVEVCTNN*