AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0647120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0647120 MtrunA17_Chr4g0035651 95.536 224 9 1 7 229 1 224 1.61e-150 419
Msa0647120 MtrunA17_Chr3g0145061 59.624 213 69 6 7 204 1 211 1.66e-76 231
Msa0647120 MtrunA17_Chr1g0155811 47.926 217 99 4 7 214 1 212 4.08e-57 184
Msa0647120 MtrunA17_Chr3g0142221 47.191 178 58 3 7 183 1 143 1.15e-50 166
Msa0647120 MtrunA17_Chr2g0314671 51.587 126 57 3 7 132 1 122 3.50e-37 132
Msa0647120 MtrunA17_Chr4g0033641 50.000 110 54 1 7 116 1 109 4.88e-34 123
Msa0647120 MtrunA17_Chr2g0329511 48.182 110 56 1 7 116 1 109 1.11e-31 117
Msa0647120 MtrunA17_Chr5g0405151 37.324 142 78 4 7 141 1 138 1.13e-22 92.0
Msa0647120 MtrunA17_Chr1g0150471 42.268 97 46 1 11 107 6 92 4.62e-19 85.1
Msa0647120 MtrunA17_Chr4g0072871 40.860 93 45 1 11 103 22 104 2.40e-16 77.8
Msa0647120 MtrunA17_Chr3g0136701 38.144 97 50 1 11 107 6 92 5.93e-16 75.9
Msa0647120 MtrunA17_Chr7g0220721 35.043 117 63 2 11 127 26 129 2.15e-15 75.1
Msa0647120 MtrunA17_Chr5g0429341 36.082 97 52 1 7 103 1 87 5.55e-14 71.2
Msa0647120 MtrunA17_Chr3g0120711 35.052 97 52 2 7 103 1 86 1.93e-11 63.5
Msa0647120 MtrunA17_Chr7g0268691 32.653 98 55 2 11 108 20 106 7.47e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0647120 AT4G39070.1 53.000 200 70 4 7 182 1 200 2.18e-58 186
Msa0647120 AT1G75540.1 45.411 207 92 4 7 193 1 206 1.18e-53 177
Msa0647120 AT1G78600.1 52.586 116 54 1 7 122 1 115 1.37e-37 134
Msa0647120 AT1G78600.2 43.103 174 90 3 7 179 1 166 2.03e-37 134
Msa0647120 AT1G06040.2 46.715 137 66 2 7 143 1 130 1.54e-36 128
Msa0647120 AT1G06040.1 46.715 137 66 2 7 143 1 130 1.65e-35 127
Msa0647120 AT2G31380.1 51.327 113 54 1 7 119 1 112 3.70e-33 121
Msa0647120 AT4G10240.1 44.138 145 67 4 7 141 1 141 2.17e-30 112
Msa0647120 AT4G38960.1 43.810 105 55 2 7 111 1 101 3.87e-23 93.6
Msa0647120 AT4G38960.6 43.810 105 55 2 7 111 1 101 3.87e-23 93.6
Msa0647120 AT2G21320.1 43.810 105 55 2 7 111 1 101 5.68e-22 90.1
Msa0647120 AT2G24790.2 41.237 97 47 1 11 107 8 94 5.63e-19 83.2
Msa0647120 AT2G24790.1 41.237 97 47 1 11 107 8 94 1.50e-18 83.6
Msa0647120 AT5G24930.1 37.113 97 51 1 11 107 6 92 4.38e-16 77.4
Msa0647120 AT5G15850.1 32.836 134 76 3 1 133 1 121 6.67e-16 77.0
Msa0647120 AT5G15840.2 33.333 123 70 2 11 133 20 130 5.93e-15 73.2
Msa0647120 AT5G15840.1 33.333 123 70 2 11 133 20 130 1.10e-14 73.6
Msa0647120 AT3G02380.1 36.559 93 49 1 11 103 16 98 1.51e-14 72.8
Msa0647120 AT1G28050.1 35.644 101 55 1 8 108 6 96 2.02e-12 67.0
Msa0647120 AT2G47890.2 40.244 82 39 1 11 92 13 84 2.56e-12 65.5
Msa0647120 AT2G47890.3 40.244 82 39 1 11 92 13 84 3.01e-12 65.9
Msa0647120 AT2G47890.1 40.244 82 39 1 11 92 13 84 4.82e-12 65.5
Msa0647120 AT5G48250.1 34.694 98 53 2 7 103 1 88 5.47e-12 65.5
Msa0647120 AT2G33500.1 33.981 103 58 1 6 108 7 99 6.19e-12 65.5
Msa0647120 AT2G33500.2 33.981 103 58 1 6 108 7 99 6.53e-12 65.5
Msa0647120 AT5G57660.1 33.858 127 61 4 11 128 22 134 1.71e-11 63.9
Msa0647120 AT3G07650.3 36.047 86 45 1 7 92 1 76 5.95e-11 62.4
Msa0647120 AT3G07650.2 36.047 86 45 1 7 92 1 76 5.95e-11 62.4
Msa0647120 AT3G07650.4 36.047 86 45 1 7 92 1 76 5.95e-11 62.4
Msa0647120 AT3G07650.1 36.047 86 45 1 7 92 1 76 5.95e-11 62.4

Find 82 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGGATCTGATGAATTA+TGG 0.164578 4_3:-53081047 None:intergenic
GGATGTGGACAAAAGAATTA+TGG 0.243412 4_3:-53081177 None:intergenic
TCCAAAGTTAATGTAGTAAA+AGG 0.273884 4_3:-53081145 None:intergenic
AATTGTTTCAATCAAGTATT+CGG 0.312961 4_3:-53082023 None:intergenic
ACCTTACAGAAAACGTTATT+AGG 0.333966 4_3:-53082088 None:intergenic
TGTGCTATTTCTACTACATT+TGG 0.337992 4_3:-53081063 None:intergenic
ATTTGGATCTGATGAATTAT+GGG 0.352913 4_3:-53081046 None:intergenic
CTTGAAAATGAACTTGGTCT+TGG 0.353565 4_3:-53081926 None:intergenic
TGTTTATAACTTGAAGATCT+TGG 0.361700 4_3:-53082256 None:intergenic
CTGAGCCATTGGAAGTTGTT+GGG 0.365142 4_3:-53081886 None:intergenic
ATCACAGAGAGGTGTGTCTT+TGG 0.365914 4_3:-53081408 None:intergenic
TTGTTCTCATCTAAGGTAGA+AGG 0.378807 4_3:+53082547 Msa0647120:three_prime_UTR
TCTGAGCCATTGGAAGTTGT+TGG 0.394596 4_3:-53081887 None:intergenic
TACTTGATTGAAACAATTCC+TGG 0.400109 4_3:+53082028 Msa0647120:CDS
AAGACAGAGCAATACTTTGC+AGG 0.405273 4_3:+53081762 Msa0647120:CDS
CAGAGAGCTGCTTCATCTGC+AGG 0.413650 4_3:-53081305 None:intergenic
GAGAGAGTCAGAAAATAAAT+AGG 0.421018 4_3:+53080906 Msa0647120:five_prime_UTR
GATGTGGACAAAAGAATTAT+GGG 0.421642 4_3:-53081176 None:intergenic
CAGAGAGAACAGCTAAGATT+TGG 0.425768 4_3:-53080994 None:intergenic
CAACATACTAAAGCTGTTGA+AGG 0.434663 4_3:+53082377 Msa0647120:CDS
TGGAAGTGGGGTGGTGAAGA+GGG 0.434963 4_3:-53081388 None:intergenic
CCCCACATTGATTCTTTAGA+CGG 0.435559 4_3:+53082341 Msa0647120:CDS
CCTCTACATTGTTTGAAGAA+TGG 0.436801 4_3:-53082425 None:intergenic
ATGGATAAGAAAACAAATGA+TGG 0.444047 4_3:-53082492 None:intergenic
GTGTAAAAGAAGCATCAAAA+AGG 0.446259 4_3:-53082066 None:intergenic
AAGTTATAAACATGTCTGAC+TGG 0.451524 4_3:+53082267 Msa0647120:CDS
AACTTACCAAGCAACACAAC+AGG 0.456566 4_3:+53081816 Msa0647120:CDS
TGAACTTGGTCTTGGACTTG+AGG 0.462812 4_3:-53081918 None:intergenic
GTGTTTAGCAGAGACCTTGT+TGG 0.467471 4_3:-53081357 None:intergenic
CTTCTCACCGGCGTAAAGAT+CGG 0.467524 4_3:+53081842 Msa0647120:CDS
CAGAGACCTTGTTGGCATAG+TGG 0.468511 4_3:-53081349 None:intergenic
CCCACATTGATTCTTTAGAC+GGG 0.468902 4_3:+53082342 Msa0647120:CDS
ATAATTCGTACATATGAAAC+GGG 0.469720 4_3:-53082607 None:intergenic
GATAATTCGTACATATGAAA+CGG 0.470506 4_3:-53082608 None:intergenic
GAGAGGTGTGTCTTTGGAAG+TGG 0.478667 4_3:-53081402 None:intergenic
GAGAGAAGTACATAATTAAG+AGG 0.479528 4_3:-53082574 None:intergenic
CGTACATATGAAACGGGAAA+AGG 0.480675 4_3:-53082601 None:intergenic
CAAGTATTCGGAAATGCTAC+TGG 0.481122 4_3:-53082011 None:intergenic
TCTGATGAATTATGGGCGTT+TGG 0.486017 4_3:-53081039 None:intergenic
GGTTCTTAGTTCTGAGCCAT+TGG 0.490954 4_3:-53081897 None:intergenic
AAGAATGGAGGACTAATTGA+AGG 0.493324 4_3:-53082410 None:intergenic
TTGGAAGTGGGGTGGTGAAG+AGG 0.494426 4_3:-53081389 None:intergenic
TGGTAAGTTTGTTGACTTCG+TGG 0.510633 4_3:-53081802 None:intergenic
GCCATTGATTTAAAATCGAT+CGG 0.523753 4_3:+53081111 Msa0647120:five_prime_UTR
TTCTCTTTCTATGAGTAAAG+AGG 0.529085 4_3:-53080874 None:intergenic
AAGAAACCTGTTGTGTTGCT+TGG 0.533532 4_3:-53081822 None:intergenic
TTGAAGGCAAAGTGGGGATG+TGG 0.535312 4_3:-53081192 None:intergenic
AACAATTCCTGGTTACTGCA+TGG 0.545989 4_3:+53082039 Msa0647120:CDS
AAAATAAATAGGTCTCGTAA+TGG 0.547529 4_3:+53080917 Msa0647120:five_prime_UTR
TGGATAAGAAAACAAATGAT+GGG 0.550125 4_3:-53082491 None:intergenic
CACAACAGGTTTCTTCTCAC+CGG 0.551440 4_3:+53081830 Msa0647120:CDS
AGTTTGTTGACTTCGTGGAT+AGG 0.553093 4_3:-53081797 None:intergenic
AAGTTCTTTCAAAGAAAACA+TGG 0.562402 4_3:+53081970 Msa0647120:CDS
AGACAGAGCAATACTTTGCA+GGG 0.562722 4_3:+53081763 Msa0647120:CDS
GAGGTGTGTCTTTGGAAGTG+GGG 0.563256 4_3:-53081400 None:intergenic
GTGTGTCTTTGGAAGTGGGG+TGG 0.564550 4_3:-53081397 None:intergenic
TCAAACCCAACAACTTCCAA+TGG 0.565279 4_3:+53081881 Msa0647120:CDS
TTCATCTGCAGGGCAAAACA+AGG 0.566264 4_3:-53081294 None:intergenic
AGGTCTTCCATGCAGTAACC+AGG 0.571610 4_3:-53082046 None:intergenic
AGAGGTGTGTCTTTGGAAGT+GGG 0.572522 4_3:-53081401 None:intergenic
CTACATTGTTTGAAGAATGG+AGG 0.582130 4_3:-53082422 None:intergenic
TCTCTTTCTATGAGTAAAGA+GGG 0.582196 4_3:-53080873 None:intergenic
CGGAATCTAACTTGAGATTG+TGG 0.587577 4_3:-53082293 None:intergenic
ATATGAAACGGGAAAAGGAA+AGG 0.590584 4_3:-53082596 None:intergenic
CCCGTCTAAAGAATCAATGT+GGG 0.595981 4_3:-53082342 None:intergenic
AGAGAGCTGCTTCATCTGCA+GGG 0.597045 4_3:-53081304 None:intergenic
TTACACGCATTAGTTGATCT+CGG 0.600288 4_3:+53081089 Msa0647120:five_prime_UTR
CAATATCCACTATGCCAACA+AGG 0.603596 4_3:+53081343 Msa0647120:CDS
CCACATTGATTCTTTAGACG+GGG 0.606501 4_3:+53082343 Msa0647120:CDS
TCGAATTAGCACTAAGCTGA+CGG 0.608767 4_3:-53082313 None:intergenic
CCCCGTCTAAAGAATCAATG+TGG 0.617525 4_3:-53082343 None:intergenic
GTTTGTGACATGCATCACAC+TGG 0.618408 4_3:-53081262 None:intergenic
ACCTCTCTGTGATATCTGCA+AGG 0.620933 4_3:+53081418 Msa0647120:CDS
TGATGCATGTCACAAACAAG+AGG 0.622158 4_3:+53081268 Msa0647120:CDS
AGTTATAAACATGTCTGACT+GGG 0.637692 4_3:+53082268 Msa0647120:CDS
CCATTCTTCAAACAATGTAG+AGG 0.655261 4_3:+53082425 Msa0647120:CDS
TCTCATCTAAGGTAGAAGGT+AGG 0.670836 4_3:+53082551 Msa0647120:three_prime_UTR
ATCTGCAGGGCAAAACAAGG+AGG 0.678639 4_3:-53081291 None:intergenic
CATAGAAAGAGAATCACACA+TGG 0.682829 4_3:+53080884 Msa0647120:five_prime_UTR
AGAAGCACCGATCTTTACGC+CGG 0.690841 4_3:-53081849 None:intergenic
CCGTCTAAAGAATCAATGTG+GGG 0.706719 4_3:-53082341 None:intergenic
ACCTTGCAGATATCACAGAG+AGG 0.780941 4_3:-53081419 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGTTTATCAATAAAAT+AGG + chr4_3:53081612-53081631 Msa0647120:intron 10.0%
!!! AATTTGTTTATCAATAAAAT+AGG + chr4_3:53081612-53081631 Msa0647120:intron 10.0%
!!! AATAAGTTGTATATGTTTTT+TGG + chr4_3:53082205-53082224 Msa0647120:intron 15.0%
!! TTCTAGATAAAAATTGTAAA+TGG - chr4_3:53082514-53082533 None:intergenic 15.0%
!!! AATAAGTTGTATATGTTTTT+TGG + chr4_3:53082205-53082224 Msa0647120:intron 15.0%
!! TTCTAGATAAAAATTGTAAA+TGG - chr4_3:53082514-53082533 None:intergenic 15.0%
!!! AACAAATATGACCATATTTT+TGG - chr4_3:53081661-53081680 None:intergenic 20.0%
!! AATAAAAGACTGAGTAATAT+AGG + chr4_3:53081691-53081710 Msa0647120:intron 20.0%
!! AATTGTTTCAATCAAGTATT+CGG - chr4_3:53082026-53082045 None:intergenic 20.0%
!!! AACAAATATGACCATATTTT+TGG - chr4_3:53081661-53081680 None:intergenic 20.0%
!! AATAAAAGACTGAGTAATAT+AGG + chr4_3:53081691-53081710 Msa0647120:intron 20.0%
!! AATTGTTTCAATCAAGTATT+CGG - chr4_3:53082026-53082045 None:intergenic 20.0%
! AAAATAAATAGGTCTCGTAA+TGG + chr4_3:53080917-53080936 Msa0647120:five_prime_UTR 25.0%
! ATCTGACTTTCAGAAAAATT+TGG - chr4_3:53080950-53080969 None:intergenic 25.0%
! ATTTGGATCTGATGAATTAT+GGG - chr4_3:53081049-53081068 None:intergenic 25.0%
!! ACCTTTTACTACATTAACTT+TGG + chr4_3:53081144-53081163 Msa0647120:five_prime_UTR 25.0%
! TCCAAAGTTAATGTAGTAAA+AGG - chr4_3:53081148-53081167 None:intergenic 25.0%
!!! CTCTTTATTTTTGAGCATTT+TGG - chr4_3:53081234-53081253 None:intergenic 25.0%
!! GGAAATAACATTTTTATTGC+AGG + chr4_3:53081712-53081731 Msa0647120:intron 25.0%
!! TTTTCACTTGAAAATGAACT+TGG - chr4_3:53081935-53081954 None:intergenic 25.0%
! AAGTTCTTTCAAAGAAAACA+TGG + chr4_3:53081970-53081989 Msa0647120:CDS 25.0%
!!! ATGTTTTTTGGTATTGTTTC+AGG + chr4_3:53082217-53082236 Msa0647120:intron 25.0%
! TGTTTATAACTTGAAGATCT+TGG - chr4_3:53082259-53082278 None:intergenic 25.0%
! TGGATAAGAAAACAAATGAT+GGG - chr4_3:53082494-53082513 None:intergenic 25.0%
! ATGGATAAGAAAACAAATGA+TGG - chr4_3:53082495-53082514 None:intergenic 25.0%
! AAAATAAATAGGTCTCGTAA+TGG + chr4_3:53080917-53080936 Msa0647120:five_prime_UTR 25.0%
! ATCTGACTTTCAGAAAAATT+TGG - chr4_3:53080950-53080969 None:intergenic 25.0%
! ATTTGGATCTGATGAATTAT+GGG - chr4_3:53081049-53081068 None:intergenic 25.0%
!! ACCTTTTACTACATTAACTT+TGG + chr4_3:53081144-53081163 Msa0647120:five_prime_UTR 25.0%
! TCCAAAGTTAATGTAGTAAA+AGG - chr4_3:53081148-53081167 None:intergenic 25.0%
!!! CTCTTTATTTTTGAGCATTT+TGG - chr4_3:53081234-53081253 None:intergenic 25.0%
!! GGAAATAACATTTTTATTGC+AGG + chr4_3:53081712-53081731 Msa0647120:intron 25.0%
!! TTTTCACTTGAAAATGAACT+TGG - chr4_3:53081935-53081954 None:intergenic 25.0%
! AAGTTCTTTCAAAGAAAACA+TGG + chr4_3:53081970-53081989 Msa0647120:CDS 25.0%
!!! ATGTTTTTTGGTATTGTTTC+AGG + chr4_3:53082217-53082236 Msa0647120:intron 25.0%
! TGTTTATAACTTGAAGATCT+TGG - chr4_3:53082259-53082278 None:intergenic 25.0%
! TGGATAAGAAAACAAATGAT+GGG - chr4_3:53082494-53082513 None:intergenic 25.0%
! ATGGATAAGAAAACAAATGA+TGG - chr4_3:53082495-53082514 None:intergenic 25.0%
TCTCTTTCTATGAGTAAAGA+GGG - chr4_3:53080876-53080895 None:intergenic 30.0%
TTCTCTTTCTATGAGTAAAG+AGG - chr4_3:53080877-53080896 None:intergenic 30.0%
GAGAGAGTCAGAAAATAAAT+AGG + chr4_3:53080906-53080925 Msa0647120:five_prime_UTR 30.0%
CATTTGGATCTGATGAATTA+TGG - chr4_3:53081050-53081069 None:intergenic 30.0%
!! TGTGCTATTTCTACTACATT+TGG - chr4_3:53081066-53081085 None:intergenic 30.0%
!! GCCATTGATTTAAAATCGAT+CGG + chr4_3:53081111-53081130 Msa0647120:five_prime_UTR 30.0%
GATGTGGACAAAAGAATTAT+GGG - chr4_3:53081179-53081198 None:intergenic 30.0%
!!! TTTTGTTTTGAAGGCAAAGT+GGG - chr4_3:53081202-53081221 None:intergenic 30.0%
!!! AGATTTGGGTTTTGTTTTGA+AGG - chr4_3:53081211-53081230 None:intergenic 30.0%
!! TTTGAGCATTTTGGAGATTT+GGG - chr4_3:53081225-53081244 None:intergenic 30.0%
!! TTTTGAGCATTTTGGAGATT+TGG - chr4_3:53081226-53081245 None:intergenic 30.0%
! TTTCTTACGTACTTTTCAAC+AGG + chr4_3:53081456-53081475 Msa0647120:intron 30.0%
! TTCTTACGTACTTTTCAACA+GGG + chr4_3:53081457-53081476 Msa0647120:intron 30.0%
TGTTGAAAATCAGTCAAAGA+AGG - chr4_3:53081564-53081583 None:intergenic 30.0%
! TAACATTTTTATTGCAGGAG+AGG + chr4_3:53081717-53081736 Msa0647120:intron 30.0%
TACTTGATTGAAACAATTCC+TGG + chr4_3:53082028-53082047 Msa0647120:CDS 30.0%
!! GTGTAAAAGAAGCATCAAAA+AGG - chr4_3:53082069-53082088 None:intergenic 30.0%
! ACCTAATAACGTTTTCTGTA+AGG + chr4_3:53082087-53082106 Msa0647120:CDS 30.0%
ACCTTACAGAAAACGTTATT+AGG - chr4_3:53082091-53082110 None:intergenic 30.0%
!! AACGTTTTCTGTAAGGTATT+TGG + chr4_3:53082094-53082113 Msa0647120:intron 30.0%
TAGATGAAAAATAGCTAGCT+AGG - chr4_3:53082172-53082191 None:intergenic 30.0%
AAGTTATAAACATGTCTGAC+TGG + chr4_3:53082267-53082286 Msa0647120:CDS 30.0%
AGTTATAAACATGTCTGACT+GGG + chr4_3:53082268-53082287 Msa0647120:CDS 30.0%
! TGCAATTTTGTTCTCATCTA+AGG + chr4_3:53082540-53082559 Msa0647120:three_prime_UTR 30.0%
! GAGAGAAGTACATAATTAAG+AGG - chr4_3:53082577-53082596 None:intergenic 30.0%
TCTCTTTCTATGAGTAAAGA+GGG - chr4_3:53080876-53080895 None:intergenic 30.0%
TTCTCTTTCTATGAGTAAAG+AGG - chr4_3:53080877-53080896 None:intergenic 30.0%
GAGAGAGTCAGAAAATAAAT+AGG + chr4_3:53080906-53080925 Msa0647120:five_prime_UTR 30.0%
CATTTGGATCTGATGAATTA+TGG - chr4_3:53081050-53081069 None:intergenic 30.0%
!! TGTGCTATTTCTACTACATT+TGG - chr4_3:53081066-53081085 None:intergenic 30.0%
!! GCCATTGATTTAAAATCGAT+CGG + chr4_3:53081111-53081130 Msa0647120:five_prime_UTR 30.0%
GATGTGGACAAAAGAATTAT+GGG - chr4_3:53081179-53081198 None:intergenic 30.0%
!!! TTTTGTTTTGAAGGCAAAGT+GGG - chr4_3:53081202-53081221 None:intergenic 30.0%
!!! AGATTTGGGTTTTGTTTTGA+AGG - chr4_3:53081211-53081230 None:intergenic 30.0%
!! TTTGAGCATTTTGGAGATTT+GGG - chr4_3:53081225-53081244 None:intergenic 30.0%
!! TTTTGAGCATTTTGGAGATT+TGG - chr4_3:53081226-53081245 None:intergenic 30.0%
! TTTCTTACGTACTTTTCAAC+AGG + chr4_3:53081456-53081475 Msa0647120:intron 30.0%
! TTCTTACGTACTTTTCAACA+GGG + chr4_3:53081457-53081476 Msa0647120:intron 30.0%
TGTTGAAAATCAGTCAAAGA+AGG - chr4_3:53081564-53081583 None:intergenic 30.0%
! TAACATTTTTATTGCAGGAG+AGG + chr4_3:53081717-53081736 Msa0647120:intron 30.0%
TACTTGATTGAAACAATTCC+TGG + chr4_3:53082028-53082047 Msa0647120:CDS 30.0%
!! GTGTAAAAGAAGCATCAAAA+AGG - chr4_3:53082069-53082088 None:intergenic 30.0%
! ACCTAATAACGTTTTCTGTA+AGG + chr4_3:53082087-53082106 Msa0647120:CDS 30.0%
ACCTTACAGAAAACGTTATT+AGG - chr4_3:53082091-53082110 None:intergenic 30.0%
!! AACGTTTTCTGTAAGGTATT+TGG + chr4_3:53082094-53082113 Msa0647120:intron 30.0%
TAGATGAAAAATAGCTAGCT+AGG - chr4_3:53082172-53082191 None:intergenic 30.0%
AAGTTATAAACATGTCTGAC+TGG + chr4_3:53082267-53082286 Msa0647120:CDS 30.0%
AGTTATAAACATGTCTGACT+GGG + chr4_3:53082268-53082287 Msa0647120:CDS 30.0%
! TGCAATTTTGTTCTCATCTA+AGG + chr4_3:53082540-53082559 Msa0647120:three_prime_UTR 30.0%
! GAGAGAAGTACATAATTAAG+AGG - chr4_3:53082577-53082596 None:intergenic 30.0%
CATAGAAAGAGAATCACACA+TGG + chr4_3:53080884-53080903 Msa0647120:five_prime_UTR 35.0%
TTACACGCATTAGTTGATCT+CGG + chr4_3:53081089-53081108 Msa0647120:five_prime_UTR 35.0%
!! GCCGATCGATTTTAAATCAA+TGG - chr4_3:53081115-53081134 None:intergenic 35.0%
GGATGTGGACAAAAGAATTA+TGG - chr4_3:53081180-53081199 None:intergenic 35.0%
!!! TTTGTTTTGAAGGCAAAGTG+GGG - chr4_3:53081201-53081220 None:intergenic 35.0%
!!! GTTTTGTTTTGAAGGCAAAG+TGG - chr4_3:53081203-53081222 None:intergenic 35.0%
!!! ACCATATTTTTGGACCATGT+AGG - chr4_3:53081651-53081670 None:intergenic 35.0%
CTTGAAAATGAACTTGGTCT+TGG - chr4_3:53081929-53081948 None:intergenic 35.0%
CAACATACTAAAGCTGTTGA+AGG + chr4_3:53082377-53082396 Msa0647120:CDS 35.0%
AAGAATGGAGGACTAATTGA+AGG - chr4_3:53082413-53082432 None:intergenic 35.0%
CTACATTGTTTGAAGAATGG+AGG - chr4_3:53082425-53082444 None:intergenic 35.0%
CCTCTACATTGTTTGAAGAA+TGG - chr4_3:53082428-53082447 None:intergenic 35.0%
CCATTCTTCAAACAATGTAG+AGG + chr4_3:53082425-53082444 Msa0647120:CDS 35.0%
! TTGTTCTCATCTAAGGTAGA+AGG + chr4_3:53082547-53082566 Msa0647120:three_prime_UTR 35.0%
ATATGAAACGGGAAAAGGAA+AGG - chr4_3:53082599-53082618 None:intergenic 35.0%
CATAGAAAGAGAATCACACA+TGG + chr4_3:53080884-53080903 Msa0647120:five_prime_UTR 35.0%
TTACACGCATTAGTTGATCT+CGG + chr4_3:53081089-53081108 Msa0647120:five_prime_UTR 35.0%
!! GCCGATCGATTTTAAATCAA+TGG - chr4_3:53081115-53081134 None:intergenic 35.0%
GGATGTGGACAAAAGAATTA+TGG - chr4_3:53081180-53081199 None:intergenic 35.0%
!!! TTTGTTTTGAAGGCAAAGTG+GGG - chr4_3:53081201-53081220 None:intergenic 35.0%
!!! GTTTTGTTTTGAAGGCAAAG+TGG - chr4_3:53081203-53081222 None:intergenic 35.0%
!!! ACCATATTTTTGGACCATGT+AGG - chr4_3:53081651-53081670 None:intergenic 35.0%
CTTGAAAATGAACTTGGTCT+TGG - chr4_3:53081929-53081948 None:intergenic 35.0%
CAACATACTAAAGCTGTTGA+AGG + chr4_3:53082377-53082396 Msa0647120:CDS 35.0%
AAGAATGGAGGACTAATTGA+AGG - chr4_3:53082413-53082432 None:intergenic 35.0%
CTACATTGTTTGAAGAATGG+AGG - chr4_3:53082425-53082444 None:intergenic 35.0%
CCTCTACATTGTTTGAAGAA+TGG - chr4_3:53082428-53082447 None:intergenic 35.0%
CCATTCTTCAAACAATGTAG+AGG + chr4_3:53082425-53082444 Msa0647120:CDS 35.0%
! TTGTTCTCATCTAAGGTAGA+AGG + chr4_3:53082547-53082566 Msa0647120:three_prime_UTR 35.0%
ATATGAAACGGGAAAAGGAA+AGG - chr4_3:53082599-53082618 None:intergenic 35.0%
CAGAGAGAACAGCTAAGATT+TGG - chr4_3:53080997-53081016 None:intergenic 40.0%
TCTGATGAATTATGGGCGTT+TGG - chr4_3:53081042-53081061 None:intergenic 40.0%
TGATGCATGTCACAAACAAG+AGG + chr4_3:53081268-53081287 Msa0647120:CDS 40.0%
CAATATCCACTATGCCAACA+AGG + chr4_3:53081343-53081362 Msa0647120:CDS 40.0%
GATGAAGATCATAGCCTACA+TGG + chr4_3:53081634-53081653 Msa0647120:intron 40.0%
GCCTACATGGTCCAAAAATA+TGG + chr4_3:53081647-53081666 Msa0647120:intron 40.0%
AAGACAGAGCAATACTTTGC+AGG + chr4_3:53081762-53081781 Msa0647120:CDS 40.0%
AGACAGAGCAATACTTTGCA+GGG + chr4_3:53081763-53081782 Msa0647120:CDS 40.0%
!! AGTTTGTTGACTTCGTGGAT+AGG - chr4_3:53081800-53081819 None:intergenic 40.0%
!! TGGTAAGTTTGTTGACTTCG+TGG - chr4_3:53081805-53081824 None:intergenic 40.0%
AACTTACCAAGCAACACAAC+AGG + chr4_3:53081816-53081835 Msa0647120:CDS 40.0%
! AAGAAACCTGTTGTGTTGCT+TGG - chr4_3:53081825-53081844 None:intergenic 40.0%
TCAAACCCAACAACTTCCAA+TGG + chr4_3:53081881-53081900 Msa0647120:CDS 40.0%
CAAGTATTCGGAAATGCTAC+TGG - chr4_3:53082014-53082033 None:intergenic 40.0%
AACAATTCCTGGTTACTGCA+TGG + chr4_3:53082039-53082058 Msa0647120:CDS 40.0%
CGGAATCTAACTTGAGATTG+TGG - chr4_3:53082296-53082315 None:intergenic 40.0%
TCGAATTAGCACTAAGCTGA+CGG - chr4_3:53082316-53082335 None:intergenic 40.0%
CCGTCTAAAGAATCAATGTG+GGG - chr4_3:53082344-53082363 None:intergenic 40.0%
!! CCCCACATTGATTCTTTAGA+CGG + chr4_3:53082341-53082360 Msa0647120:CDS 40.0%
CCCGTCTAAAGAATCAATGT+GGG - chr4_3:53082345-53082364 None:intergenic 40.0%
!! CCCACATTGATTCTTTAGAC+GGG + chr4_3:53082342-53082361 Msa0647120:CDS 40.0%
!! CCACATTGATTCTTTAGACG+GGG + chr4_3:53082343-53082362 Msa0647120:CDS 40.0%
! TCTCATCTAAGGTAGAAGGT+AGG + chr4_3:53082551-53082570 Msa0647120:three_prime_UTR 40.0%
CGTACATATGAAACGGGAAA+AGG - chr4_3:53082604-53082623 None:intergenic 40.0%
CAGAGAGAACAGCTAAGATT+TGG - chr4_3:53080997-53081016 None:intergenic 40.0%
TCTGATGAATTATGGGCGTT+TGG - chr4_3:53081042-53081061 None:intergenic 40.0%
TGATGCATGTCACAAACAAG+AGG + chr4_3:53081268-53081287 Msa0647120:CDS 40.0%
CAATATCCACTATGCCAACA+AGG + chr4_3:53081343-53081362 Msa0647120:CDS 40.0%
GATGAAGATCATAGCCTACA+TGG + chr4_3:53081634-53081653 Msa0647120:intron 40.0%
GCCTACATGGTCCAAAAATA+TGG + chr4_3:53081647-53081666 Msa0647120:intron 40.0%
AAGACAGAGCAATACTTTGC+AGG + chr4_3:53081762-53081781 Msa0647120:CDS 40.0%
AGACAGAGCAATACTTTGCA+GGG + chr4_3:53081763-53081782 Msa0647120:CDS 40.0%
!! AGTTTGTTGACTTCGTGGAT+AGG - chr4_3:53081800-53081819 None:intergenic 40.0%
!! TGGTAAGTTTGTTGACTTCG+TGG - chr4_3:53081805-53081824 None:intergenic 40.0%
AACTTACCAAGCAACACAAC+AGG + chr4_3:53081816-53081835 Msa0647120:CDS 40.0%
! AAGAAACCTGTTGTGTTGCT+TGG - chr4_3:53081825-53081844 None:intergenic 40.0%
TCAAACCCAACAACTTCCAA+TGG + chr4_3:53081881-53081900 Msa0647120:CDS 40.0%
CAAGTATTCGGAAATGCTAC+TGG - chr4_3:53082014-53082033 None:intergenic 40.0%
AACAATTCCTGGTTACTGCA+TGG + chr4_3:53082039-53082058 Msa0647120:CDS 40.0%
CGGAATCTAACTTGAGATTG+TGG - chr4_3:53082296-53082315 None:intergenic 40.0%
TCGAATTAGCACTAAGCTGA+CGG - chr4_3:53082316-53082335 None:intergenic 40.0%
CCGTCTAAAGAATCAATGTG+GGG - chr4_3:53082344-53082363 None:intergenic 40.0%
!! CCCCACATTGATTCTTTAGA+CGG + chr4_3:53082341-53082360 Msa0647120:CDS 40.0%
CCCGTCTAAAGAATCAATGT+GGG - chr4_3:53082345-53082364 None:intergenic 40.0%
!! CCCACATTGATTCTTTAGAC+GGG + chr4_3:53082342-53082361 Msa0647120:CDS 40.0%
!! CCACATTGATTCTTTAGACG+GGG + chr4_3:53082343-53082362 Msa0647120:CDS 40.0%
! TCTCATCTAAGGTAGAAGGT+AGG + chr4_3:53082551-53082570 Msa0647120:three_prime_UTR 40.0%
CGTACATATGAAACGGGAAA+AGG - chr4_3:53082604-53082623 None:intergenic 40.0%
GTTTGTGACATGCATCACAC+TGG - chr4_3:53081265-53081284 None:intergenic 45.0%
TTCATCTGCAGGGCAAAACA+AGG - chr4_3:53081297-53081316 None:intergenic 45.0%
GTGTTTAGCAGAGACCTTGT+TGG - chr4_3:53081360-53081379 None:intergenic 45.0%
! AGAGGTGTGTCTTTGGAAGT+GGG - chr4_3:53081404-53081423 None:intergenic 45.0%
! ATCACAGAGAGGTGTGTCTT+TGG - chr4_3:53081411-53081430 None:intergenic 45.0%
ACCTCTCTGTGATATCTGCA+AGG + chr4_3:53081418-53081437 Msa0647120:CDS 45.0%
ACCTTGCAGATATCACAGAG+AGG - chr4_3:53081422-53081441 None:intergenic 45.0%
CACAACAGGTTTCTTCTCAC+CGG + chr4_3:53081830-53081849 Msa0647120:CDS 45.0%
CTGAGCCATTGGAAGTTGTT+GGG - chr4_3:53081889-53081908 None:intergenic 45.0%
TCTGAGCCATTGGAAGTTGT+TGG - chr4_3:53081890-53081909 None:intergenic 45.0%
!! GGTTCTTAGTTCTGAGCCAT+TGG - chr4_3:53081900-53081919 None:intergenic 45.0%
!! TGAACTTGGTCTTGGACTTG+AGG - chr4_3:53081921-53081940 None:intergenic 45.0%
CCCCGTCTAAAGAATCAATG+TGG - chr4_3:53082346-53082365 None:intergenic 45.0%
GTTTGTGACATGCATCACAC+TGG - chr4_3:53081265-53081284 None:intergenic 45.0%
TTCATCTGCAGGGCAAAACA+AGG - chr4_3:53081297-53081316 None:intergenic 45.0%
GTGTTTAGCAGAGACCTTGT+TGG - chr4_3:53081360-53081379 None:intergenic 45.0%
! AGAGGTGTGTCTTTGGAAGT+GGG - chr4_3:53081404-53081423 None:intergenic 45.0%
! ATCACAGAGAGGTGTGTCTT+TGG - chr4_3:53081411-53081430 None:intergenic 45.0%
ACCTCTCTGTGATATCTGCA+AGG + chr4_3:53081418-53081437 Msa0647120:CDS 45.0%
ACCTTGCAGATATCACAGAG+AGG - chr4_3:53081422-53081441 None:intergenic 45.0%
CACAACAGGTTTCTTCTCAC+CGG + chr4_3:53081830-53081849 Msa0647120:CDS 45.0%
CTGAGCCATTGGAAGTTGTT+GGG - chr4_3:53081889-53081908 None:intergenic 45.0%
TCTGAGCCATTGGAAGTTGT+TGG - chr4_3:53081890-53081909 None:intergenic 45.0%
!! GGTTCTTAGTTCTGAGCCAT+TGG - chr4_3:53081900-53081919 None:intergenic 45.0%
!! TGAACTTGGTCTTGGACTTG+AGG - chr4_3:53081921-53081940 None:intergenic 45.0%
CCCCGTCTAAAGAATCAATG+TGG - chr4_3:53082346-53082365 None:intergenic 45.0%
TTGAAGGCAAAGTGGGGATG+TGG - chr4_3:53081195-53081214 None:intergenic 50.0%
ATCTGCAGGGCAAAACAAGG+AGG - chr4_3:53081294-53081313 None:intergenic 50.0%
AGAGAGCTGCTTCATCTGCA+GGG - chr4_3:53081307-53081326 None:intergenic 50.0%
CAGAGACCTTGTTGGCATAG+TGG - chr4_3:53081352-53081371 None:intergenic 50.0%
! GAGGTGTGTCTTTGGAAGTG+GGG - chr4_3:53081403-53081422 None:intergenic 50.0%
! GAGAGGTGTGTCTTTGGAAG+TGG - chr4_3:53081405-53081424 None:intergenic 50.0%
CTGCAACACAACTGCAGTTG+AGG + chr4_3:53081529-53081548 Msa0647120:intron 50.0%
CTTCTCACCGGCGTAAAGAT+CGG + chr4_3:53081842-53081861 Msa0647120:CDS 50.0%
!! AGAAGCACCGATCTTTACGC+CGG - chr4_3:53081852-53081871 None:intergenic 50.0%
AGGTCTTCCATGCAGTAACC+AGG - chr4_3:53082049-53082068 None:intergenic 50.0%
TTGAAGGCAAAGTGGGGATG+TGG - chr4_3:53081195-53081214 None:intergenic 50.0%
ATCTGCAGGGCAAAACAAGG+AGG - chr4_3:53081294-53081313 None:intergenic 50.0%
AGAGAGCTGCTTCATCTGCA+GGG - chr4_3:53081307-53081326 None:intergenic 50.0%
CAGAGACCTTGTTGGCATAG+TGG - chr4_3:53081352-53081371 None:intergenic 50.0%
! GAGGTGTGTCTTTGGAAGTG+GGG - chr4_3:53081403-53081422 None:intergenic 50.0%
! GAGAGGTGTGTCTTTGGAAG+TGG - chr4_3:53081405-53081424 None:intergenic 50.0%
CTGCAACACAACTGCAGTTG+AGG + chr4_3:53081529-53081548 Msa0647120:intron 50.0%
CTTCTCACCGGCGTAAAGAT+CGG + chr4_3:53081842-53081861 Msa0647120:CDS 50.0%
!! AGAAGCACCGATCTTTACGC+CGG - chr4_3:53081852-53081871 None:intergenic 50.0%
AGGTCTTCCATGCAGTAACC+AGG - chr4_3:53082049-53082068 None:intergenic 50.0%
CAGAGAGCTGCTTCATCTGC+AGG - chr4_3:53081308-53081327 None:intergenic 55.0%
TGGAAGTGGGGTGGTGAAGA+GGG - chr4_3:53081391-53081410 None:intergenic 55.0%
TTGGAAGTGGGGTGGTGAAG+AGG - chr4_3:53081392-53081411 None:intergenic 55.0%
! GTGTGTCTTTGGAAGTGGGG+TGG - chr4_3:53081400-53081419 None:intergenic 55.0%
CAGAGAGCTGCTTCATCTGC+AGG - chr4_3:53081308-53081327 None:intergenic 55.0%
TGGAAGTGGGGTGGTGAAGA+GGG - chr4_3:53081391-53081410 None:intergenic 55.0%
TTGGAAGTGGGGTGGTGAAG+AGG - chr4_3:53081392-53081411 None:intergenic 55.0%
! GTGTGTCTTTGGAAGTGGGG+TGG - chr4_3:53081400-53081419 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 53080873 53082627 53080873 ID=Msa0647120;Name=Msa0647120
chr4_3 mRNA 53080873 53082627 53080873 ID=Msa0647120-mRNA-1;Parent=Msa0647120;Name=Msa0647120-mRNA-1;_AED=0.05;_eAED=0.05;_QI=363|1|1|1|1|1|3|170|265
chr4_3 exon 53080873 53081439 53080873 ID=Msa0647120-mRNA-1:exon:13108;Parent=Msa0647120-mRNA-1
chr4_3 exon 53081734 53082108 53081734 ID=Msa0647120-mRNA-1:exon:13109;Parent=Msa0647120-mRNA-1
chr4_3 exon 53082239 53082627 53082239 ID=Msa0647120-mRNA-1:exon:13110;Parent=Msa0647120-mRNA-1
chr4_3 five_prime_UTR 53080873 53081235 53080873 ID=Msa0647120-mRNA-1:five_prime_utr;Parent=Msa0647120-mRNA-1
chr4_3 CDS 53081236 53081439 53081236 ID=Msa0647120-mRNA-1:cds;Parent=Msa0647120-mRNA-1
chr4_3 CDS 53081734 53082108 53081734 ID=Msa0647120-mRNA-1:cds;Parent=Msa0647120-mRNA-1
chr4_3 CDS 53082239 53082457 53082239 ID=Msa0647120-mRNA-1:cds;Parent=Msa0647120-mRNA-1
chr4_3 three_prime_UTR 53082458 53082627 53082458 ID=Msa0647120-mRNA-1:three_prime_utr;Parent=Msa0647120-mRNA-1
Gene Sequence

>Msa0647120

ATGCTCAAAAATAAAGAGATGAAGATCCAGTGTGATGCATGTCACAAACAAGAGGCCTCCTTGTTTTGCCCTGCAGATGAAGCAGCTCTCTGCAATCAATGTGATCGCAATATCCACTATGCCAACAAGGTCTCTGCTAAACACAAACGCTTCCCTCTTCACCACCCCACTTCCAAAGACACACCTCTCTGTGATATCTGCAAGGAGAGGCGTGCATATCTATTTTGCAAAGAAGACAGAGCAATACTTTGCAGGGAATGTGACATTCCTATCCACGAAGTCAACAAACTTACCAAGCAACACAACAGGTTTCTTCTCACCGGCGTAAAGATCGGTGCTTCTTCTTCTTATTCAAACCCAACAACTTCCAATGGCTCAGAACTAAGAACCTCAAGTCCAAGACCAAGTTCATTTTCAAGTGAAAATAATAGTTGTTCTCAAAGTTCTTTCAAAGAAAACATGGCTTGTGACACAGTTTCAACCAGTAGCATTTCCGAATACTTGATTGAAACAATTCCTGGTTACTGCATGGAAGACCTTTTTGATGCTTCTTTTACACCTAATAACGTTTTCTGTAAGGATTATCATGAACAGAACCAAGATCTTCAAGTTATAAACATGTCTGACTGGGTACCACAATCTCAAGTTAGATTCCGTCAGCTTAGTGCTAATTCGAATGTTCCCCACATTGATTCTTTAGACGGGGTTATGAAAATGCAACATACTAAAGCTGTTGAAGGATACAAAGTTCCTTCAATTAGTCCTCCATTCTTCAAACAATGTAGAGGCACTCGTTAA
Protein sequence

>Msa0647120

MLKNKEMKIQCDACHKQEASLFCPADEAALCNQCDRNIHYANKVSAKHKRFPLHHPTSKDTPLCDICKERRAYLFCKEDRAILCRECDIPIHEVNKLTKQHNRFLLTGVKIGASSSYSNPTTSNGSELRTSSPRPSSFSSENNSCSQSSFKENMACDTVSTSSISEYLIETIPGYCMEDLFDASFTPNNVFCKDYHEQNQDLQVINMSDWVPQSQVRFRQLSANSNVPHIDSLDGVMKMQHTKAVEGYKVPSISPPFFKQCRGTR*