AlfalfaGEDB | Msa0648550

M. sativa cultivar zhongmu-4 / Msa0648550

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648550	XP_024630856.2	87.337	766	39	2	1	735	14	752	0.0	1378

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648550	sp\|Q9SWG3\|FAR1_ARATH	34.123	633	380	12	1	601	55	682	1.46e-118	378

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648550	Q2HSZ7	74.479	768	154	7	1	734	356	1115	0.0	1170

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TFs/TRs:

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Gene ID	Type	Classification
Msa0648550	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648550	MtrunA17_Chr2g0296781	69.471	737	182	6	1	704	1	727	0.0	1028
Msa0648550	MtrunA17_Chr1g0196541	59.530	766	244	5	1	735	1	731	0.0	912
Msa0648550	MtrunA17_Chr6g0477261	69.673	643	139	5	113	701	1	641	0.0	892
Msa0648550	MtrunA17_Chr5g0441581	45.950	605	284	6	1	573	36	629	0.0	538
Msa0648550	MtrunA17_Chr2g0291581	36.655	562	307	8	1	527	75	622	6.99e-117	367
Msa0648550	MtrunA17_Chr5g0448761	36.555	569	321	11	1	531	64	630	1.42e-114	364
Msa0648550	MtrunA17_Chr1g0202341	35.702	563	319	11	1	527	55	610	1.31e-113	358
Msa0648550	MtrunA17_Chr1g0207551	34.712	556	333	5	1	528	38	591	2.72e-113	361
Msa0648550	MtrunA17_Chr2g0329031	35.231	562	320	10	1	531	18	566	4.33e-111	353
Msa0648550	MtrunA17_Chr3g0080351	34.501	571	306	10	1	528	80	625	2.26e-106	343

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648550	AT4G15090.1	34.123	633	380	12	1	601	55	682	1.48e-119	378
Msa0648550	AT4G15090.5	34.123	633	380	12	1	601	55	682	1.48e-119	378
Msa0648550	AT4G15090.3	34.123	633	380	12	1	601	55	682	1.48e-119	378
Msa0648550	AT4G15090.2	34.123	633	380	12	1	601	55	682	1.48e-119	378
Msa0648550	AT4G15090.4	34.123	633	380	12	1	601	55	682	1.48e-119	378
Msa0648550	AT4G38180.1	34.286	560	332	8	1	526	77	634	1.43e-108	348
Msa0648550	AT1G76320.3	34.859	568	326	10	1	534	1	558	1.16e-106	340
Msa0648550	AT1G76320.2	34.859	568	326	10	1	534	1	558	2.23e-106	341
Msa0648550	AT1G76320.1	34.859	568	326	10	1	534	1	558	2.99e-106	341
Msa0648550	AT1G76320.4	34.859	568	326	10	1	534	1	558	2.99e-106	341

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Find 95 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATCTTGATGTTAATC+AGG	0.381504	4_3:+55033414	Msa0648550:CDS
AAAGTGAGGCGGCTTCGACT+TGG	0.518819	4_3:+55031617	Msa0648550:CDS
AAATGATTTATCGAAAGTAA+TGG	0.527562	4_3:+55032938	Msa0648550:CDS
AAGAATTATGCAAGATGTAT+AGG	0.307421	4_3:+55031188	Msa0648550:CDS
AAGGGCGTCCTCCTTCAAAA+AGG	0.415773	4_3:+55033084	Msa0648550:CDS
AAGTGAGGCGGCTTCGACTT+GGG	0.432744	4_3:+55031618	Msa0648550:CDS
AAGTGATTTCATGGAGAAGT+GGG	0.549527	4_3:+55032165	Msa0648550:CDS
AAGTTGATCAAATTGTGAAA+AGG	0.546898	4_3:+55033117	Msa0648550:CDS
AATGTATGCGTGTCTTCAGA+TGG	0.540668	4_3:+55031362	Msa0648550:CDS
AATGTGTCTCTCACATACAC+AGG	0.631924	4_3:-55032265	Msa0648540:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGTATAAAAAGGAGATTGG+AGG	+	chr4_3:55031481-55031500	Msa0648550:CDS	30.0%
GGATATGATAACCTTACATT+CGG	+	chr4_3:55031566-55031585	Msa0648550:CDS	30.0%
GATATGATAACCTTACATTC+GGG	+	chr4_3:55031567-55031586	Msa0648550:CDS	30.0%
ATATGATAACCTTACATTCG+GGG	+	chr4_3:55031568-55031587	Msa0648550:CDS	30.0%
TGATGATTTACTCCAACAAA+AGG	-	chr4_3:55031854-55031873	Msa0648540:intron	30.0%
TTTGTTGGAGTAAATCATCA+TGG	+	chr4_3:55031854-55031873	Msa0648550:CDS	30.0%
CTTGTGGCATATAATGAAAA+AGG	+	chr4_3:55032060-55032079	Msa0648550:CDS	30.0%
TTTGAGCATAAGTGATTTCA+TGG	+	chr4_3:55032156-55032175	Msa0648550:CDS	30.0%
CTTGGCAATATATAACAAGA+GGG	-	chr4_3:55032780-55032799	Msa0648540:intron	30.0%
TCTTGGCAATATATAACAAG+AGG	-	chr4_3:55032781-55032800	Msa0648540:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	55031149	55033704	55031149	ID=Msa0648550;Name=Msa0648550
chr4_3	mRNA	55031149	55033704	55031149	ID=Msa0648550-mRNA-1;Parent=Msa0648550;Name=Msa0648550-mRNA-1;_AED=0.25;_eAED=0.37;_QI=0\|0\|0\|0.4\|1\|1\|5\|0\|735
chr4_3	exon	55031149	55032624	55031149	ID=Msa0648550-mRNA-1:exon:13973;Parent=Msa0648550-mRNA-1
chr4_3	exon	55032717	55033205	55032717	ID=Msa0648550-mRNA-1:exon:13974;Parent=Msa0648550-mRNA-1
chr4_3	exon	55033286	55033435	55033286	ID=Msa0648550-mRNA-1:exon:13975;Parent=Msa0648550-mRNA-1
chr4_3	exon	55033528	55033584	55033528	ID=Msa0648550-mRNA-1:exon:13976;Parent=Msa0648550-mRNA-1
chr4_3	exon	55033669	55033704	55033669	ID=Msa0648550-mRNA-1:exon:13977;Parent=Msa0648550-mRNA-1
chr4_3	CDS	55031149	55032624	55031149	ID=Msa0648550-mRNA-1:cds;Parent=Msa0648550-mRNA-1
chr4_3	CDS	55032717	55033205	55032717	ID=Msa0648550-mRNA-1:cds;Parent=Msa0648550-mRNA-1
chr4_3	CDS	55033286	55033435	55033286	ID=Msa0648550-mRNA-1:cds;Parent=Msa0648550-mRNA-1
chr4_3	CDS	55033528	55033584	55033528	ID=Msa0648550-mRNA-1:cds;Parent=Msa0648550-mRNA-1
chr4_3	CDS	55033669	55033704	55033669	ID=Msa0648550-mRNA-1:cds;Parent=Msa0648550-mRNA-1

Gene Sequence

>Msa0648550

ATGAAATTTAATTCTGCAGAGGAAGTTGACAAATATTACAAGAATTATGCAAGATGTATAGGTTTTGGAGTTAGCATAATCAGTACAAAAAAGGGAGATGATGGAAAAAAGTATTTTACTCTAGGGTGTAACCGTGCAAGAAGCTATGTGAGTAAGTCCAAAAATCTTTTAAAGCCAAATCCTAGCATGAAAACACACTGTAAGGCTAGAATAAATGTATGCGTGTCTTCAGATGGAACAACTACTATTTCTAAGGTTGCTCTTGAACATAATCATGACTTAATAAGTCCAAGCAAATCAAGATATTTTAGATGTAACAAGATTATTGACCCTAGTATAAAAAGGAGATTGGAGGTTAATGACCAAGCTGGGATTAATGTAAGTAGAAATTTTCGGTCTATGGTTGTTGAAGCAAGTGGATATGATAACCTTACATTCGGGGAAAAAGATGTTCGAAACTACATTGACAAAGTGAGGCGGCTTCGACTTGGGACAGGAGACGCTGAAGCCATTCAAAATTATTTTGTTAGAATGCAAAGACAAAATAATGAATTTTATTATGTAATGGATGTGGATGACAAAAGTCGTTTACGAAATGTTTTTTGGGCAGATGCGAGATGTAGGTCTGCTTATGAGTATTTTGGCGAAGTAATAACCTTTGACACCACTTACTTAACAAATAAATATGACATGCCTTTTGCTCCTTTTGTTGGAGTAAATCATCATGGTCAATCAGTTCTGTTAGGTTGCGCCCTATTGTCGAAGGAGGATACTAAAACTTTTTCTTGGTTGTTCACTACTTGGTTAGAATGTATGCGTGGACGTGCTCCACAAGCCATAATTACTGACCAGGATGGAGCAATGAAGAATGCAGTTGAGGTCGTCTTCCCGAAAGCTCGCCATCGGTGGTGCTTGTGGCATATAATGAAAAAGGTTCCAGAAAAATTGAGTAGACTCTCTAACTATGAGTCTATCAAAATACTTTTGCATGATGCGGTGTATGATTCTTTGAGCATAAGTGATTTCATGGAGAAGTGGGAAAAAATGATTGAATGTTATGAACTTCATGATAATGAATGGTTGAAAGGGTTGTTTGATGAGCGTCATCGTTGGGTTCCTGTGTATGTGAGAGACACATTTTGGGCTGGAATGTCAACTACACAACGAAGTGAAAGTATAAACGCATTTTTTGATGGATATGTGCACTCAAAGACATCATTGAAGCAATTTGTTGAACAATATGATAATGCATTGAAAGATAAAATTGAAAAGGAAAATATGGCTGATTTTAGTTCATCCAATAGAATTATTGCTTGTATAAGTCATTTTGGATTTGAATCTCAATTTCAAAAGGCATATACCAATGCAAAATTCAAAGAGTTTCAAGTAGAAGTGGCTTCTATGATGTACTGTCATGCTTTCTTTGAGAGAATGGAGGCTTTGAACTTAACATATTGTGTTACAGAAAGTAAAAAGGGTATTTTATGTAGACATATCCTTTGTGTTCTTAAGCTCACAGCTAAAACAGAGTCAGTGCCCTCTTGTTATATATTGCCAAGATGGAGGAAGGATATTAAGCGAAGATATACACTTATTAAAAGTGGTTTTGATCAATTAGCCGGAAATGAAGAGTTGCAGCGTGTAGCTAAAGCTTGTGATGCCTTTTACGAAGTTGCTTCTTCATGTATCCATACTGAAAATGATTTATCGAAAGTAATGGATAGAATAGAGAACTTAAAACTGGAGATGACATGTCAAGAAAGTTTTTCAGAGATCACTGAAGGAGATAACTTAGTTCAAAATCAGATAACTAAAATTCTTGATCCTGTAGTGACTCGAAGTAAAGGGCGTCCTCCTTCAAAAAGGAAGGCTTCTAAAGTTGATCAAATTGTGAAAAGGAAGCTTGCTGGAAAGAAAGGACAAAAAGGCAACAAAAAAAGTAACACTTATCAAAGTCAAGAAGAGGGGCCTAGTATATCTAAAGTTCAAGAAAATCAAGAGTTCTCTATATCTCAGACCATTGATCTTGATGTGATTGACACACAAGAGAGCATTCAAGTAAAACAAATGTTAGCTCCCTTCAATCCAAATCAAAAATATCTTGATGTTAATCAGGTTCCATATTCTTCTCAAAGCATAGACCACAATGTTTCATATTTAGAACTTTTACAGGCACAGCACCATATAAATGATCATTCATCATCATGA

Protein sequence

>Msa0648550

MKFNSAEEVDKYYKNYARCIGFGVSIISTKKGDDGKKYFTLGCNRARSYVSKSKNLLKPNPSMKTHCKARINVCVSSDGTTTISKVALEHNHDLISPSKSRYFRCNKIIDPSIKRRLEVNDQAGINVSRNFRSMVVEASGYDNLTFGEKDVRNYIDKVRRLRLGTGDAEAIQNYFVRMQRQNNEFYYVMDVDDKSRLRNVFWADARCRSAYEYFGEVITFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSKEDTKTFSWLFTTWLECMRGRAPQAIITDQDGAMKNAVEVVFPKARHRWCLWHIMKKVPEKLSRLSNYESIKILLHDAVYDSLSISDFMEKWEKMIECYELHDNEWLKGLFDERHRWVPVYVRDTFWAGMSTTQRSESINAFFDGYVHSKTSLKQFVEQYDNALKDKIEKENMADFSSSNRIIACISHFGFESQFQKAYTNAKFKEFQVEVASMMYCHAFFERMEALNLTYCVTESKKGILCRHILCVLKLTAKTESVPSCYILPRWRKDIKRRYTLIKSGFDQLAGNEELQRVAKACDAFYEVASSCIHTENDLSKVMDRIENLKLEMTCQESFSEITEGDNLVQNQITKILDPVVTRSKGRPPSKRKASKVDQIVKRKLAGKKGQKGNKKSNTYQSQEEGPSISKVQENQEFSISQTIDLDVIDTQESIQVKQMLAPFNPNQKYLDVNQVPYSSQSIDHNVSYLELLQAQHHINDHSSS*