AlfalfaGEDB | Msa0649340

M. sativa cultivar zhongmu-4 / Msa0649340

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649340	AES89785.2	91.987	312	16	2	1	309	1	306	0.0	514

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649340	sp\|Q8VZI9\|ENAP1_ARATH	40.614	293	120	7	13	305	9	247	3.73e-48	164

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0649340	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649340	MtrunA17_Chr4g0041151	92.857	308	13	2	1	305	1	302	0.0	511
Msa0649340	MtrunA17_Chr2g0283871	52.090	311	119	7	19	306	11	314	9.43e-87	263
Msa0649340	MtrunA17_Chr8g0344681	38.567	293	144	7	27	300	64	339	3.07e-45	157
Msa0649340	MtrunA17_Chr2g0325841	33.924	395	159	11	8	308	23	409	1.35e-39	148
Msa0649340	MtrunA17_Chr2g0331931	30.345	290	144	7	14	296	255	493	2.49e-26	108
Msa0649340	MtrunA17_Chr1g0179531	37.255	102	57	3	31	131	133	228	1.53e-16	80.1
Msa0649340	MtrunA17_Chr6g0485241	38.947	95	52	2	31	124	130	219	1.87e-16	79.0
Msa0649340	MtrunA17_Chr3g0082261	25.524	286	177	9	26	294	33	299	7.96e-15	73.9
Msa0649340	MtrunA17_Chr4g0066271	35.922	103	63	2	30	131	116	216	2.15e-14	73.2
Msa0649340	MtrunA17_Chr1g0164461	33.333	102	60	2	26	127	33	126	4.20e-12	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649340	AT3G58630.2	47.239	326	123	9	1	302	1	301	6.67e-74	230
Msa0649340	AT3G58630.1	47.239	326	123	9	1	302	1	301	1.07e-73	230
Msa0649340	AT3G11100.1	40.614	293	120	7	13	305	9	247	3.79e-49	164
Msa0649340	AT5G05550.3	40.143	279	113	6	27	305	20	244	3.42e-48	162
Msa0649340	AT5G05550.1	40.143	279	113	6	27	305	20	244	4.14e-48	162
Msa0649340	AT5G05550.2	40.288	278	112	6	27	304	20	243	5.02e-48	161
Msa0649340	AT3G14180.1	59.615	104	38	2	27	130	80	179	2.78e-34	130
Msa0649340	AT1G54060.1	51.485	101	44	2	27	127	90	185	8.38e-22	95.1
Msa0649340	AT3G24490.1	27.925	265	139	6	31	295	90	302	1.01e-21	94.0
Msa0649340	AT3G10030.2	39.669	121	66	3	16	134	144	259	1.54e-20	92.4

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Find 110 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGCACTAAACGTGCTTC+TGG	0.443118	4_3:-55960025	Msa0649340:CDS
AAAGAGACGAAGCATCGTTC+CGG	0.461522	4_3:-55960342	Msa0649340:CDS
AAAGTGTGGAGTCAGAGGAA+TGG	0.557513	4_3:-55960270	Msa0649340:CDS
AACTCAACAACCGCGTTTCA+TGG	0.344366	4_3:-55960926	Msa0649340:exon
AAGAAAGGAAGAGAAAACGA+TGG	0.648071	4_3:-55960241	Msa0649340:CDS
ACAGAGACAAATGGTGGAAT+TGG	0.329589	4_3:-55960134	Msa0649340:CDS
ACCCCAGGCGTCGATGAGGG+TGG	0.576167	4_3:+55960798	None:intergenic
ACCGTCTCCACCGTCGCCTC+CGG	0.494561	4_3:-55960428	Msa0649340:CDS
ACGGCGGTTACCAGAGTCCC+TGG	0.554940	4_3:-55960588	Msa0649340:CDS
ACGGGGAACGGCGATGACGA+CGG	0.590204	4_3:+55960853	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTGAGTTTGGAAAATCGA+CGG	+	chr4_3:55959936-55959955	None:intergenic	35.0%
AAGAAAGGAAGAGAAAACGA+TGG	-	chr4_3:55960632-55960651	Msa0649340:CDS	35.0%
AGAAAGGAAGAGAAAACGAT+GGG	-	chr4_3:55960633-55960652	Msa0649340:CDS	35.0%
AGCTTCAAAACAGAGACAAA+TGG	-	chr4_3:55960730-55960749	Msa0649340:CDS	35.0%
TTCAAAACAGAGACAAATGG+TGG	-	chr4_3:55960733-55960752	Msa0649340:CDS	35.0%
TCAGAGAATGCAGTTATACA+TGG	-	chr4_3:55960799-55960818	Msa0649340:CDS	35.0%
TGTTGAGTTTGGAAAATCGA+CGG	+	chr4_3:55959936-55959955	None:intergenic	35.0%
AAGAAAGGAAGAGAAAACGA+TGG	-	chr4_3:55960632-55960651	Msa0649340:CDS	35.0%
AGAAAGGAAGAGAAAACGAT+GGG	-	chr4_3:55960633-55960652	Msa0649340:CDS	35.0%
AGCTTCAAAACAGAGACAAA+TGG	-	chr4_3:55960730-55960749	Msa0649340:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	55959837	55961058	55959837	ID=Msa0649340;Name=Msa0649340
chr4_3	mRNA	55959837	55961058	55959837	ID=Msa0649340-mRNA-1;Parent=Msa0649340;Name=Msa0649340-mRNA-1;_AED=0.13;_eAED=0.13;_QI=129\|0\|0.5\|0.5\|1\|1\|2\|0\|316
chr4_3	exon	55959837	55959877	55959837	ID=Msa0649340-mRNA-1:exon:14446;Parent=Msa0649340-mRNA-1
chr4_3	exon	55960020	55961058	55960020	ID=Msa0649340-mRNA-1:exon:14445;Parent=Msa0649340-mRNA-1
chr4_3	five_prime_UTR	55960930	55961058	55960930	ID=Msa0649340-mRNA-1:five_prime_utr;Parent=Msa0649340-mRNA-1
chr4_3	CDS	55960020	55960929	55960020	ID=Msa0649340-mRNA-1:cds;Parent=Msa0649340-mRNA-1
chr4_3	CDS	55959837	55959877	55959837	ID=Msa0649340-mRNA-1:cds;Parent=Msa0649340-mRNA-1

Gene Sequence

>Msa0649340

ATGGACATCATCGCCGGAGCTCCGTCACCTATCAACTCCGTTCCGCCGCGTCTTCCGTCGTCATCGCCGTTCCCCGTCCGCGAAGACTGCTGGACCGAAGACGCAACTTCCACCCTCATCGACGCCTGGGGTGAACGCTACCTCGACCTTAACCGCGGTAACCTTCGTCAGAAAACTTGGCAAGAGGTTGCCGACGCCGTCAACGACGTCCACGCCGCCGGTAACAAGAAAGCGCGACGTACCGACGTTCAGTGCAAAAACCGAATCGACACGTTAAAGAAGAAGTACAAGATCGAGAAAGCTAGGGTTTCCGAATCCGACGGCGGTTACCAGAGTCCCTGGCCGTTTTTCACTCGTCTTGACGTTCTCATCGGCGATACTTTTCCGGTCAAAAAACTCTCCCCGCCGGTGAATGTACGGTCTACTCCAACCGTCGTCAAACGTCCACCTCCTCAATCTCCACCGTCTCCACCTTCTCCACCGTCTCCACCGTCGCCTCCGGCGTGGATAATCTCTCATCCGGTCGGTCCCCGATCCGGAACACAAAAACGTCCGGCAATGGCGAAAAGAGACGAAGCATCGTTCCGGCGGAATTTCTCGGCGTTCGCGGCGGCTGCTGCGGCGGCGGCTGAGGCAGAAAGTGTGGAGTCAGAGGAATGGAGGGGGAAGAAAGGAAGAGAAAACGATGGGACCCATAAGGAGTTTGGTTTCAGAGAGCTCGCTCAGGCTATAGAGAGATTCAGTGAAGTATATGAAAGAGTTGAAGCTTCAAAACAGAGACAAATGGTGGAATTGGAGAAGCAGAGGATGCAGTTTGTGAAGGATTTGGAGTATCAGAGAATGCAGTTATACATGGAAACGCAGGTTCAGCTTCAGAAAATCAAACGCACTAAACGTGCTTCTGGTTCCGCAGAAGTTGGGTATTTTGTTACTTGTGATAATGGTAACTGA

Protein sequence

>Msa0649340

MDIIAGAPSPINSVPPRLPSSSPFPVREDCWTEDATSTLIDAWGERYLDLNRGNLRQKTWQEVADAVNDVHAAGNKKARRTDVQCKNRIDTLKKKYKIEKARVSESDGGYQSPWPFFTRLDVLIGDTFPVKKLSPPVNVRSTPTVVKRPPPQSPPSPPSPPSPPSPPAWIISHPVGPRSGTQKRPAMAKRDEASFRRNFSAFAAAAAAAAEAESVESEEWRGKKGRENDGTHKEFGFRELAQAIERFSEVYERVEASKQRQMVELEKQRMQFVKDLEYQRMQLYMETQVQLQKIKRTKRASGSAEVGYFVTCDNGN*