AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0649370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0649370 MtrunA17_Chr4g0041301 100.000 142 0 0 1 142 1 142 5.50e-101 285
Msa0649370 MtrunA17_Chr2g0320211 91.304 138 12 0 5 142 3 140 2.67e-89 256
Msa0649370 MtrunA17_Chr6g0485281 50.435 115 57 0 4 118 9 123 5.81e-34 115
Msa0649370 MtrunA17_Chr6g0457491 87.273 55 7 0 88 142 1 55 7.87e-28 97.8
Msa0649370 MtrunA17_Chr7g0275831 90.000 40 4 0 92 131 1 40 5.29e-19 75.5
Msa0649370 MtrunA17_Chr4g0057871 55.844 77 21 1 32 95 9 85 2.33e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0649370 AT3G58680.1 84.507 142 22 0 1 142 1 142 1.32e-86 249
Msa0649370 AT2G42680.1 85.915 142 20 0 1 142 1 142 1.77e-86 249
Msa0649370 AT3G24500.1 50.360 139 67 1 5 141 7 145 1.09e-43 141
Msa0649370 AT3G24500.2 43.820 89 48 1 5 91 7 95 4.84e-17 71.6

Find 71 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCATTGGCCTGGTTTGC+AGG 0.172981 4_3:+56024571 Msa0649370:three_prime_UTR
ATTGGTGTTCAAGAATTAAT+TGG 0.217200 4_3:-56024598 None:intergenic
GACGGCTTTCTCATCTTTCT+TGG 0.250063 4_3:-56022101 None:intergenic
ATCTTTCTTGGCGGCGGCGT+TGG 0.250269 4_3:-56022089 None:intergenic
TTGCTAATTATCTGCTGGTT+TGG 0.292459 4_3:-56024277 None:intergenic
CCATCACTGTGGCTGTTAAA+AGG 0.293647 4_3:+56024755 Msa0649370:three_prime_UTR
GTTCCCAATCTTGAGAAATA+TGG 0.320777 4_3:-56022042 None:intergenic
CTGTGTTTATCTTGGTTCAT+TGG 0.327924 4_3:+56024558 Msa0649370:three_prime_UTR
TAGCTCAGTGAAGGTGTAAA+TGG 0.330318 4_3:-56021953 None:intergenic
TTTCTTGACGGTATCGATAT+CGG 0.338092 4_3:-56022146 None:intergenic
TCTTTCTTGGCGGCGGCGTT+GGG 0.340591 4_3:-56022088 None:intergenic
GGCACGGCGAGCGGCGTTGA+CGG 0.351560 4_3:-56022119 None:intergenic
GTCAACGCCGCTCGCCGTGC+CGG 0.353656 4_3:+56022121 Msa0649370:CDS
GGGTTTGCATGTCACTCTTA+AGG 0.362036 4_3:+56024368 Msa0649370:three_prime_UTR
GTATATGTAAAACATATAAA+TGG 0.389403 4_3:+56024423 Msa0649370:three_prime_UTR
TGACAAGTACTCTGCAAATC+AGG 0.389884 4_3:-56024662 None:intergenic
TAGGCCATATTTCTCAAGAT+TGG 0.393130 4_3:+56022038 Msa0649370:CDS
AGCAAGTTGGAGAGAGCCCT+TGG 0.404653 4_3:+56024295 Msa0649370:CDS
GTTCATTGGCCTGGTTTGCA+GGG 0.414765 4_3:+56024572 Msa0649370:three_prime_UTR
TTGGTGTTCAAGAATTAATT+GGG 0.418275 4_3:-56024597 None:intergenic
CCAACTTGCTAATTATCTGC+TGG 0.425113 4_3:-56024282 None:intergenic
GTGATCCAAGAGTACGAGTC+AGG 0.425391 4_3:+56024244 Msa0649370:CDS
CTTTCCCTGACTCGTACTCT+TGG 0.428066 4_3:-56024249 None:intergenic
AAGTTACAAATTTCAAACTT+AGG 0.430321 4_3:-56024632 None:intergenic
ATTTCGCTTATTCAAACAAC+AGG 0.430737 4_3:-56024465 None:intergenic
AGGCCATATTTCTCAAGATT+GGG 0.431239 4_3:+56022039 Msa0649370:CDS
TTCTTGAACACCAATGGACT+TGG 0.442586 4_3:+56024606 Msa0649370:three_prime_UTR
ATCGGCGCCGGCACGGCGAG+CGG 0.444792 4_3:-56022128 None:intergenic
GCTTTCTTGCGGATGACGAC+TGG 0.447489 4_3:-56022064 None:intergenic
CTGATTTCATGATTGTGCGC+TGG 0.453008 4_3:+56024347 Msa0649370:three_prime_UTR
AGTGAGTGCTGTGTTTATCT+TGG 0.458752 4_3:+56024550 Msa0649370:three_prime_UTR
AATTATCTGCTGGTTTGGAA+TGG 0.474147 4_3:-56024272 None:intergenic
GAGATAAACATGTCAGGTCT+AGG 0.474885 4_3:+56022019 Msa0649370:exon
CAGCGCACAATCATGAAATC+AGG 0.477136 4_3:-56024346 None:intergenic
TTTATCTTGGTTCATTGGCC+TGG 0.479258 4_3:+56024563 Msa0649370:three_prime_UTR
TGATTTCATGATTGTGCGCT+GGG 0.486261 4_3:+56024348 Msa0649370:three_prime_UTR
GCACTTCATTGAACACTAAG+AGG 0.487095 4_3:+56022488 Msa0649370:CDS
GTGATGGTGAAGGTGGTTCA+TGG 0.487129 4_3:-56024739 None:intergenic
AATTGGGTCCCCTGCAAACC+AGG 0.492034 4_3:-56024581 None:intergenic
GGCTATAATGCAAGCTAGGA+TGG 0.493126 4_3:+56024082 Msa0649370:CDS
TTGAAAATCACTTGAAAGGA+GGG 0.498570 4_3:-56024505 None:intergenic
AATTAATTCTTGAACACCAA+TGG 0.503254 4_3:+56024600 Msa0649370:three_prime_UTR
TTCATTGAACACTAAGAGGC+TGG 0.503842 4_3:+56022492 Msa0649370:CDS
TGCCACGCAGTTTAGCTCCA+AGG 0.505877 4_3:-56024312 None:intergenic
CCAGCAGATAATTAGCAAGT+TGG 0.509376 4_3:+56024282 Msa0649370:CDS
AACAAAGAGATAAACATGTC+AGG 0.512918 4_3:+56022013 Msa0649370:exon
ATAGCCTTCTTGAGTTCAGT+TGG 0.514235 4_3:-56024065 None:intergenic
AGAAGGCTATAATGCAAGCT+AGG 0.515044 4_3:+56024078 Msa0649370:CDS
TTCATTGGCCTGGTTTGCAG+GGG 0.520676 4_3:+56024573 Msa0649370:three_prime_UTR
GAGGAGCGAACGTTTCTTGA+CGG 0.526730 4_3:-56022158 None:intergenic
CGGCGTTGGGAGCTTTCTTG+CGG 0.528475 4_3:-56022075 None:intergenic
GCCACGCAGTTTAGCTCCAA+GGG 0.531362 4_3:-56024311 None:intergenic
GGCTTTCTCATCTTTCTTGG+CGG 0.532271 4_3:-56022098 None:intergenic
ACTTAGGATTCCAAGTCCAT+TGG 0.537779 4_3:-56024616 None:intergenic
CTTGAAAATCACTTGAAAGG+AGG 0.538825 4_3:-56024506 None:intergenic
TACCTTGAAAATCACTTGAA+AGG 0.542425 4_3:-56024509 None:intergenic
GAACACTAAGAGGCTGGATG+AGG 0.556069 4_3:+56022498 Msa0649370:CDS
GACGGTATCGATATCGGCGC+CGG 0.571328 4_3:-56022140 None:intergenic
TAGGGTTCGTAGCTCAGTGA+AGG 0.583142 4_3:-56021962 None:intergenic
TTTCTCATCTTTCTTGGCGG+CGG 0.585231 4_3:-56022095 None:intergenic
TGTACCAACTGAACTCAAGA+AGG 0.594355 4_3:+56024061 Msa0649370:CDS
TCGTACTCTTGGATCACTTG+AGG 0.598699 4_3:-56024238 None:intergenic
AGCCACAGTGATGGTGAAGG+TGG 0.614245 4_3:-56024746 None:intergenic
TACGAACCCTAAACAGACAC+AGG 0.616571 4_3:+56021974 Msa0649370:five_prime_UTR
TGATCCAAGAGTACGAGTCA+GGG 0.620671 4_3:+56024245 Msa0649370:CDS
TATCGATATCGGCGCCGGCA+CGG 0.624694 4_3:-56022135 None:intergenic
CAAACAACAGGTGAAAATCA+CGG 0.639797 4_3:-56024453 None:intergenic
AACAGCCACAGTGATGGTGA+AGG 0.642564 4_3:-56024749 None:intergenic
GCCCTTGGAGCTAAACTGCG+TGG 0.653713 4_3:+56024310 Msa0649370:CDS
AACCACCTTCACCATCACTG+TGG 0.758790 4_3:+56024744 Msa0649370:three_prime_UTR
TCACTGTGGCTGTTAAAAGG+TGG 0.786020 4_3:+56024758 Msa0649370:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTAATTAATTAGTTTT+GGG - chr4_3:56022186-56022205 None:intergenic 10.0%
!!! AAACATTAATTAATTAGTTT+TGG - chr4_3:56022187-56022206 None:intergenic 10.0%
!!! TTAATGTTTTTTTTAACTCT+TGG + chr4_3:56022198-56022217 Msa0649370:intron 15.0%
!!! TTCGTAGATAAAAATAATTT+TGG + chr4_3:56022311-56022330 Msa0649370:intron 15.0%
!!! TTTGATGATTATTTTGTATT+AGG + chr4_3:56022335-56022354 Msa0649370:intron 15.0%
!!! TTGATGATTATTTTGTATTA+GGG + chr4_3:56022336-56022355 Msa0649370:intron 15.0%
!! TAATTTCAATATATTGATCA+TGG + chr4_3:56022377-56022396 Msa0649370:intron 15.0%
!! TTCAAATTATATAATCTGAT+GGG - chr4_3:56023533-56023552 None:intergenic 15.0%
!! TTTCAAATTATATAATCTGA+TGG - chr4_3:56023534-56023553 None:intergenic 15.0%
!! GTATATGTAAAACATATAAA+TGG + chr4_3:56024423-56024442 Msa0649370:three_prime_UTR 15.0%
!!! TGATGATTATTTTGTATTAG+GGG + chr4_3:56022337-56022356 Msa0649370:intron 20.0%
!!! TTTTTGAATGAGTAGTTATA+TGG + chr4_3:56022633-56022652 Msa0649370:intron 20.0%
!!! TTTTGAATGAGTAGTTATAT+GGG + chr4_3:56022634-56022653 Msa0649370:intron 20.0%
!!! TTCATATAGAAAATTTTGTG+TGG + chr4_3:56022709-56022728 Msa0649370:intron 20.0%
!!! ATTTTGAATTCATGATGTTT+TGG + chr4_3:56022892-56022911 Msa0649370:intron 20.0%
!! CAGAAAAGAAGAAAAAAAAA+TGG - chr4_3:56023113-56023132 None:intergenic 20.0%
!! AGGAGAAATAAAAAAATAAC+TGG + chr4_3:56023257-56023276 Msa0649370:intron 20.0%
!! GGAGAAATAAAAAAATAACT+GGG + chr4_3:56023258-56023277 Msa0649370:intron 20.0%
!! TCAAATTATATAATCTGATG+GGG - chr4_3:56023532-56023551 None:intergenic 20.0%
!! AACCCAATACATATAAATTA+CGG - chr4_3:56023718-56023737 None:intergenic 20.0%
!! CATTTCCCTAAAAAATATAA+TGG - chr4_3:56023972-56023991 None:intergenic 20.0%
!! CATGTTATCAATAACAAAAA+TGG + chr4_3:56024190-56024209 Msa0649370:intron 20.0%
!! AAGTTACAAATTTCAAACTT+AGG - chr4_3:56024635-56024654 None:intergenic 20.0%
!!! AATTAATTAGTTTTGGGAAG+AGG - chr4_3:56022180-56022199 None:intergenic 25.0%
! TTTGAATGAGTAGTTATATG+GGG + chr4_3:56022635-56022654 Msa0649370:intron 25.0%
! ATATGATTCAAACTCAATCA+CGG + chr4_3:56023083-56023102 Msa0649370:intron 25.0%
! TAGATTTCGAAATCAAATTG+TGG + chr4_3:56023181-56023200 Msa0649370:intron 25.0%
! AGATTTCGAAATCAAATTGT+GGG + chr4_3:56023182-56023201 Msa0649370:intron 25.0%
! ATTAAGTAAATGCTAAACAG+TGG - chr4_3:56023326-56023345 None:intergenic 25.0%
! AGCATTTACTTAATTGTTTG+TGG + chr4_3:56023332-56023351 Msa0649370:intron 25.0%
! GCATTTACTTAATTGTTTGT+GGG + chr4_3:56023333-56023352 Msa0649370:intron 25.0%
! CATTTACTTAATTGTTTGTG+GGG + chr4_3:56023334-56023353 Msa0649370:intron 25.0%
! TCTCCGTAATTTATATGTAT+TGG + chr4_3:56023712-56023731 Msa0649370:intron 25.0%
! CTCCGTAATTTATATGTATT+GGG + chr4_3:56023713-56023732 Msa0649370:intron 25.0%
!! TCATGATCAAAATTTTCTCT+TGG + chr4_3:56023801-56023820 Msa0649370:intron 25.0%
! ATATCATGCTTAATAGATAG+AGG + chr4_3:56023935-56023954 Msa0649370:intron 25.0%
!!! TGTCCACCATTATATTTTTT+AGG + chr4_3:56023963-56023982 Msa0649370:intron 25.0%
!!! GTCCACCATTATATTTTTTA+GGG + chr4_3:56023964-56023983 Msa0649370:intron 25.0%
! TTCCCTAAAAAATATAATGG+TGG - chr4_3:56023969-56023988 None:intergenic 25.0%
!! TTGGTGTTCAAGAATTAATT+GGG - chr4_3:56024600-56024619 None:intergenic 25.0%
!! ATTGGTGTTCAAGAATTAAT+TGG - chr4_3:56024601-56024620 None:intergenic 25.0%
! AATTAATTCTTGAACACCAA+TGG + chr4_3:56024600-56024619 Msa0649370:three_prime_UTR 25.0%
AACAAAGAGATAAACATGTC+AGG + chr4_3:56022013-56022032 Msa0649370:exon 30.0%
TATGAAAATACTTTAGCTGC+AGG - chr4_3:56022695-56022714 None:intergenic 30.0%
! AAAATTTTGTGTGGAACATG+TGG + chr4_3:56022718-56022737 Msa0649370:intron 30.0%
TTCTACAGCATAAATTTGAG+CGG - chr4_3:56022946-56022965 None:intergenic 30.0%
!!! AACATATTTTGAGTTGTGCT+TGG - chr4_3:56022988-56023007 None:intergenic 30.0%
CAAAATATGTTTGCATAGCT+TGG + chr4_3:56022997-56023016 Msa0649370:intron 30.0%
CAGAAAACAGGACTAAAATA+TGG + chr4_3:56023222-56023241 Msa0649370:intron 30.0%
! AATTATAGGGTGGATTTGAA+TGG + chr4_3:56023358-56023377 Msa0649370:intron 30.0%
! AAATTTTCTCTTGGCTTCAT+TGG + chr4_3:56023810-56023829 Msa0649370:intron 30.0%
AATAAAGCAACTGAACAATC+AGG - chr4_3:56023853-56023872 None:intergenic 30.0%
TAAAGAACATAAAGAGACTG+AGG - chr4_3:56024160-56024179 None:intergenic 30.0%
ATTTCGCTTATTCAAACAAC+AGG - chr4_3:56024468-56024487 None:intergenic 30.0%
TTGAAAATCACTTGAAAGGA+GGG - chr4_3:56024508-56024527 None:intergenic 30.0%
TACCTTGAAAATCACTTGAA+AGG - chr4_3:56024512-56024531 None:intergenic 30.0%
!!! AACCTTGTTTTTTCGACAAA+TGG - chr4_3:56024712-56024731 None:intergenic 30.0%
! TGTTTTCCTGTGTCTGTTTA+GGG - chr4_3:56021983-56022002 None:intergenic 35.0%
TAGGCCATATTTCTCAAGAT+TGG + chr4_3:56022038-56022057 Msa0649370:CDS 35.0%
AGGCCATATTTCTCAAGATT+GGG + chr4_3:56022039-56022058 Msa0649370:CDS 35.0%
GTTCCCAATCTTGAGAAATA+TGG - chr4_3:56022045-56022064 None:intergenic 35.0%
!! TTTCTTGACGGTATCGATAT+CGG - chr4_3:56022149-56022168 None:intergenic 35.0%
! GGTTGTGTGTTTTGTGATTT+TGG + chr4_3:56022398-56022417 Msa0649370:intron 35.0%
! GTGTGTTTTGTGATTTTGGA+TGG + chr4_3:56022402-56022421 Msa0649370:intron 35.0%
! TGATTTTGGATGGTGATTGT+TGG + chr4_3:56022412-56022431 Msa0649370:intron 35.0%
CCATTGATAGAATAGAACCT+CGG + chr4_3:56022604-56022623 Msa0649370:intron 35.0%
!! CTTTAAGCACTGCTAACTTT+TGG + chr4_3:56022832-56022851 Msa0649370:intron 35.0%
GCTTGGACTAAAAATCAACA+TGG - chr4_3:56022971-56022990 None:intergenic 35.0%
! AAATATGGCTTGTACACTCA+AGG + chr4_3:56023237-56023256 Msa0649370:intron 35.0%
ATTGTTTGTGGGGCAATTAT+AGG + chr4_3:56023344-56023363 Msa0649370:intron 35.0%
TTGTTTGTGGGGCAATTATA+GGG + chr4_3:56023345-56023364 Msa0649370:intron 35.0%
! CATTGAAACTGTTTTCGCAA+AGG + chr4_3:56023412-56023431 Msa0649370:intron 35.0%
TGTATTGGAAGTCCATTTAG+CGG + chr4_3:56023455-56023474 Msa0649370:intron 35.0%
ACTGTCCTTGTAAAGTTGAA+TGG + chr4_3:56023648-56023667 Msa0649370:intron 35.0%
GCTAACCATTCAACTTTACA+AGG - chr4_3:56023656-56023675 None:intergenic 35.0%
AGTGACAACAAAGACGTTAA+AGG - chr4_3:56023686-56023705 None:intergenic 35.0%
ATCTCATCAAGCATGAGTTA+AGG - chr4_3:56023746-56023765 None:intergenic 35.0%
! AGGGAAATGTTAACCACTTT+TGG + chr4_3:56023983-56024002 Msa0649370:intron 35.0%
AACATAAAGAGACTGAGGTT+GGG - chr4_3:56024155-56024174 None:intergenic 35.0%
!! AATTATCTGCTGGTTTGGAA+TGG - chr4_3:56024275-56024294 None:intergenic 35.0%
! TTGCTAATTATCTGCTGGTT+TGG - chr4_3:56024280-56024299 None:intergenic 35.0%
CAAACAACAGGTGAAAATCA+CGG - chr4_3:56024456-56024475 None:intergenic 35.0%
CTTGAAAATCACTTGAAAGG+AGG - chr4_3:56024509-56024528 None:intergenic 35.0%
!! CTCCTTTCAAGTGATTTTCA+AGG + chr4_3:56024507-56024526 Msa0649370:three_prime_UTR 35.0%
CTGTGTTTATCTTGGTTCAT+TGG + chr4_3:56024558-56024577 Msa0649370:three_prime_UTR 35.0%
CTCCATTTGTCGAAAAAACA+AGG + chr4_3:56024707-56024726 Msa0649370:three_prime_UTR 35.0%
! TAGCTCAGTGAAGGTGTAAA+TGG - chr4_3:56021956-56021975 None:intergenic 40.0%
! GTGTTTTCCTGTGTCTGTTT+AGG - chr4_3:56021984-56022003 None:intergenic 40.0%
GAGATAAACATGTCAGGTCT+AGG + chr4_3:56022019-56022038 Msa0649370:exon 40.0%
CGTATTGCGATTGTTGTAGT+TGG + chr4_3:56022228-56022247 Msa0649370:intron 40.0%
GCACTTCATTGAACACTAAG+AGG + chr4_3:56022488-56022507 Msa0649370:CDS 40.0%
TTCATTGAACACTAAGAGGC+TGG + chr4_3:56022492-56022511 Msa0649370:CDS 40.0%
CCGAGGTTCTATTCTATCAA+TGG - chr4_3:56022607-56022626 None:intergenic 40.0%
TCATTCAAAAATCGACACCG+AGG - chr4_3:56022624-56022643 None:intergenic 40.0%
GTTATATGGGGAGAATGATG+AGG + chr4_3:56022647-56022666 Msa0649370:intron 40.0%
TTTGTGTGGAACATGTGGTT+TGG + chr4_3:56022723-56022742 Msa0649370:intron 40.0%
ATGTTTGCATAGCTTGGACA+TGG + chr4_3:56023003-56023022 Msa0649370:intron 40.0%
TGTTTGCATAGCTTGGACAT+GGG + chr4_3:56023004-56023023 Msa0649370:intron 40.0%
TCAAATTGTGGGTCAAGTTC+CGG + chr4_3:56023193-56023212 Msa0649370:intron 40.0%
CAATGCATGAATCCGCTAAA+TGG - chr4_3:56023470-56023489 None:intergenic 40.0%
AGAGAGAGCGATCACATAAT+GGG - chr4_3:56023567-56023586 None:intergenic 40.0%
! TCTTGCTGTGTGTGTAATTG+TGG + chr4_3:56023590-56023609 Msa0649370:intron 40.0%
!! ACACAGCATGCTTTGGAATT+TGG + chr4_3:56023893-56023912 Msa0649370:intron 40.0%
TCTGTTGACAATCACATGAC+TGG + chr4_3:56024017-56024036 Msa0649370:intron 40.0%
TGTACCAACTGAACTCAAGA+AGG + chr4_3:56024061-56024080 Msa0649370:CDS 40.0%
ATAGCCTTCTTGAGTTCAGT+TGG - chr4_3:56024068-56024087 None:intergenic 40.0%
AGAAGGCTATAATGCAAGCT+AGG + chr4_3:56024078-56024097 Msa0649370:CDS 40.0%
ACATAAAGAGACTGAGGTTG+GGG - chr4_3:56024154-56024173 None:intergenic 40.0%
GAACATAAAGAGACTGAGGT+TGG - chr4_3:56024156-56024175 None:intergenic 40.0%
!! CCAACTTGCTAATTATCTGC+TGG - chr4_3:56024285-56024304 None:intergenic 40.0%
CCAGCAGATAATTAGCAAGT+TGG + chr4_3:56024282-56024301 Msa0649370:CDS 40.0%
TGATTTCATGATTGTGCGCT+GGG + chr4_3:56024348-56024367 Msa0649370:three_prime_UTR 40.0%
! AGTGAGTGCTGTGTTTATCT+TGG + chr4_3:56024550-56024569 Msa0649370:three_prime_UTR 40.0%
TTTATCTTGGTTCATTGGCC+TGG + chr4_3:56024563-56024582 Msa0649370:three_prime_UTR 40.0%
! TTCTTGAACACCAATGGACT+TGG + chr4_3:56024606-56024625 Msa0649370:three_prime_UTR 40.0%
ACTTAGGATTCCAAGTCCAT+TGG - chr4_3:56024619-56024638 None:intergenic 40.0%
TGACAAGTACTCTGCAAATC+AGG - chr4_3:56024665-56024684 None:intergenic 40.0%
TACGAACCCTAAACAGACAC+AGG + chr4_3:56021974-56021993 Msa0649370:five_prime_UTR 45.0%
TTTCTCATCTTTCTTGGCGG+CGG - chr4_3:56022098-56022117 None:intergenic 45.0%
GGCTTTCTCATCTTTCTTGG+CGG - chr4_3:56022101-56022120 None:intergenic 45.0%
GACGGCTTTCTCATCTTTCT+TGG - chr4_3:56022104-56022123 None:intergenic 45.0%
!! TGTCTACTGACTCTTGCAAC+TGG + chr4_3:56022758-56022777 Msa0649370:intron 45.0%
TTGGTCCAATGTCCTCTAGT+AGG + chr4_3:56023036-56023055 Msa0649370:intron 45.0%
CGTTACCTACTAGAGGACAT+TGG - chr4_3:56023044-56023063 None:intergenic 45.0%
AGTCTGTCGTTACCTACTAG+AGG - chr4_3:56023051-56023070 None:intergenic 45.0%
TTCCGGATCATGCAGAAAAC+AGG + chr4_3:56023210-56023229 Msa0649370:intron 45.0%
! GTCCTGTTTTCTGCATGATC+CGG - chr4_3:56023215-56023234 None:intergenic 45.0%
TTTGTGGGGCAATTATAGGG+TGG + chr4_3:56023348-56023367 Msa0649370:intron 45.0%
! GTTTTCGCAAAGGAAAGCCT+TGG + chr4_3:56023422-56023441 Msa0649370:intron 45.0%
!! AAAGGAAAGCCTTGGCTCTA+AGG + chr4_3:56023430-56023449 Msa0649370:intron 45.0%
CAATACAAGCCTTAGAGCCA+AGG - chr4_3:56023442-56023461 None:intergenic 45.0%
!! CTTGGCTCTAAGGCTTGTAT+TGG + chr4_3:56023440-56023459 Msa0649370:intron 45.0%
GAGAGAGCGATCACATAATG+GGG - chr4_3:56023566-56023585 None:intergenic 45.0%
GAGAGAGAGCGATCACATAA+TGG - chr4_3:56023568-56023587 None:intergenic 45.0%
ATTGTGCACACAGCATGCTT+TGG + chr4_3:56023886-56023905 Msa0649370:intron 45.0%
GATGTGCAGGAATCCAAAAG+TGG - chr4_3:56023999-56024018 None:intergenic 45.0%
GTGATTGTCAACAGATGTGC+AGG - chr4_3:56024012-56024031 None:intergenic 45.0%
GGCTATAATGCAAGCTAGGA+TGG + chr4_3:56024082-56024101 Msa0649370:CDS 45.0%
ATAAAGAGACTGAGGTTGGG+GGG - chr4_3:56024152-56024171 None:intergenic 45.0%
CATAAAGAGACTGAGGTTGG+GGG - chr4_3:56024153-56024172 None:intergenic 45.0%
TCGTACTCTTGGATCACTTG+AGG - chr4_3:56024241-56024260 None:intergenic 45.0%
! TGATCCAAGAGTACGAGTCA+GGG + chr4_3:56024245-56024264 Msa0649370:CDS 45.0%
CAGCGCACAATCATGAAATC+AGG - chr4_3:56024349-56024368 None:intergenic 45.0%
CTGATTTCATGATTGTGCGC+TGG + chr4_3:56024347-56024366 Msa0649370:three_prime_UTR 45.0%
GGGTTTGCATGTCACTCTTA+AGG + chr4_3:56024368-56024387 Msa0649370:three_prime_UTR 45.0%
! CCTTTTAACAGCCACAGTGA+TGG - chr4_3:56024758-56024777 None:intergenic 45.0%
CCATCACTGTGGCTGTTAAA+AGG + chr4_3:56024755-56024774 Msa0649370:three_prime_UTR 45.0%
TCACTGTGGCTGTTAAAAGG+TGG + chr4_3:56024758-56024777 Msa0649370:three_prime_UTR 45.0%
!!! ATTTTTTTTTATATCTTAAT+TGG - chr4_3:56024845-56024864 None:intergenic 5.0%
TAGGGTTCGTAGCTCAGTGA+AGG - chr4_3:56021965-56021984 None:intergenic 50.0%
GAGGAGCGAACGTTTCTTGA+CGG - chr4_3:56022161-56022180 None:intergenic 50.0%
GAACACTAAGAGGCTGGATG+AGG + chr4_3:56022498-56022517 Msa0649370:CDS 50.0%
! TGGACATGGGAGTGTGATCT+TGG + chr4_3:56023017-56023036 Msa0649370:intron 50.0%
! GTGATCCAAGAGTACGAGTC+AGG + chr4_3:56024244-56024263 Msa0649370:CDS 50.0%
! CTTTCCCTGACTCGTACTCT+TGG - chr4_3:56024252-56024271 None:intergenic 50.0%
! GTTCATTGGCCTGGTTTGCA+GGG + chr4_3:56024572-56024591 Msa0649370:three_prime_UTR 50.0%
! TTCATTGGCCTGGTTTGCAG+GGG + chr4_3:56024573-56024592 Msa0649370:three_prime_UTR 50.0%
! GTGATGGTGAAGGTGGTTCA+TGG - chr4_3:56024742-56024761 None:intergenic 50.0%
AACCACCTTCACCATCACTG+TGG + chr4_3:56024744-56024763 Msa0649370:three_prime_UTR 50.0%
! AACAGCCACAGTGATGGTGA+AGG - chr4_3:56024752-56024771 None:intergenic 50.0%
GCTTTCTTGCGGATGACGAC+TGG - chr4_3:56022067-56022086 None:intergenic 55.0%
! AGCAAGTTGGAGAGAGCCCT+TGG + chr4_3:56024295-56024314 Msa0649370:CDS 55.0%
! GCCACGCAGTTTAGCTCCAA+GGG - chr4_3:56024314-56024333 None:intergenic 55.0%
! TGCCACGCAGTTTAGCTCCA+AGG - chr4_3:56024315-56024334 None:intergenic 55.0%
! GGTTCATTGGCCTGGTTTGC+AGG + chr4_3:56024571-56024590 Msa0649370:three_prime_UTR 55.0%
AATTGGGTCCCCTGCAAACC+AGG - chr4_3:56024584-56024603 None:intergenic 55.0%
! AGCCACAGTGATGGTGAAGG+TGG - chr4_3:56024749-56024768 None:intergenic 55.0%
! CGGCGTTGGGAGCTTTCTTG+CGG - chr4_3:56022078-56022097 None:intergenic 60.0%
TCTTTCTTGGCGGCGGCGTT+GGG - chr4_3:56022091-56022110 None:intergenic 60.0%
ATCTTTCTTGGCGGCGGCGT+TGG - chr4_3:56022092-56022111 None:intergenic 60.0%
!! TATCGATATCGGCGCCGGCA+CGG - chr4_3:56022138-56022157 None:intergenic 60.0%
!! GACGGTATCGATATCGGCGC+CGG - chr4_3:56022143-56022162 None:intergenic 60.0%
GCCCTTGGAGCTAAACTGCG+TGG + chr4_3:56024310-56024329 Msa0649370:CDS 60.0%
! GGCACGGCGAGCGGCGTTGA+CGG - chr4_3:56022122-56022141 None:intergenic 75.0%
GTCAACGCCGCTCGCCGTGC+CGG + chr4_3:56022121-56022140 Msa0649370:CDS 75.0%
!!! ATCGGCGCCGGCACGGCGAG+CGG - chr4_3:56022131-56022150 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 56021944 56024878 56021944 ID=Msa0649370;Name=Msa0649370
chr4_3 mRNA 56021944 56024878 56021944 ID=Msa0649370-mRNA-1;Parent=Msa0649370;Name=Msa0649370-mRNA-1;_AED=0.04;_eAED=0.04;_QI=84|1|1|1|1|1|4|536|142
chr4_3 exon 56021944 56022169 56021944 ID=Msa0649370-mRNA-1:exon:14462;Parent=Msa0649370-mRNA-1
chr4_3 exon 56022455 56022538 56022455 ID=Msa0649370-mRNA-1:exon:14463;Parent=Msa0649370-mRNA-1
chr4_3 exon 56024054 56024136 56024054 ID=Msa0649370-mRNA-1:exon:14464;Parent=Msa0649370-mRNA-1
chr4_3 exon 56024223 56024878 56024223 ID=Msa0649370-mRNA-1:exon:14465;Parent=Msa0649370-mRNA-1
chr4_3 five_prime_UTR 56021944 56022027 56021944 ID=Msa0649370-mRNA-1:five_prime_utr;Parent=Msa0649370-mRNA-1
chr4_3 CDS 56022028 56022169 56022028 ID=Msa0649370-mRNA-1:cds;Parent=Msa0649370-mRNA-1
chr4_3 CDS 56022455 56022538 56022455 ID=Msa0649370-mRNA-1:cds;Parent=Msa0649370-mRNA-1
chr4_3 CDS 56024054 56024136 56024054 ID=Msa0649370-mRNA-1:cds;Parent=Msa0649370-mRNA-1
chr4_3 CDS 56024223 56024342 56024223 ID=Msa0649370-mRNA-1:cds;Parent=Msa0649370-mRNA-1
chr4_3 three_prime_UTR 56024343 56024878 56024343 ID=Msa0649370-mRNA-1:three_prime_utr;Parent=Msa0649370-mRNA-1
Gene Sequence

>Msa0649370

ATGTCAGGTCTAGGCCATATTTCTCAAGATTGGGAACCAGTCGTCATCCGCAAGAAAGCTCCCAACGCCGCCGCCAAGAAAGATGAGAAAGCCGTCAACGCCGCTCGCCGTGCCGGCGCCGATATCGATACCGTCAAGAAACATAATGCTGCAACAAACAAAGCTGCATCTAGCAGCACTTCATTGAACACTAAGAGGCTGGATGAGGATACTGAGAATCTAGCTCATGATCGTGTACCAACTGAACTCAAGAAGGCTATAATGCAAGCTAGGATGGACAAAAAGCTTACTCAGTCTCAGCTTGCTCAAATCATCAATGAGAAGCCTCAAGTGATCCAAGAGTACGAGTCAGGGAAAGCCATTCCAAACCAGCAGATAATTAGCAAGTTGGAGAGAGCCCTTGGAGCTAAACTGCGTGGCAAGAAATGA
Protein sequence

>Msa0649370

MSGLGHISQDWEPVVIRKKAPNAAAKKDEKAVNAARRAGADIDTVKKHNAATNKAASSSTSLNTKRLDEDTENLAHDRVPTELKKAIMQARMDKKLTQSQLAQIINEKPQVIQEYESGKAIPNQQIISKLERALGAKLRGKK*