AlfalfaGEDB | Msa0651450

M. sativa cultivar zhongmu-4 / Msa0651450

KEGG: K09260
GO: GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009555 GO:0009653 GO:0009791 GO:0009889 GO:0009908 GO:0010016 GO:0010152 GO:0010229 GO:0010468 GO:0010556 GO:0010622 GO:0010769 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022603 GO:0022604 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035670 GO:0040008 GO:0043900 GO:0045595 GO:0048229 GO:0048281 GO:0048283 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048444 GO:0048445 GO:0048467 GO:0048481 GO:0048482 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051239 GO:0051252 GO:0051510 GO:0060255 GO:0060284 GO:0061458 GO:0065007 GO:0080090 GO:0080092 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000112 GO:2000241 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0651450	KEH30865.1	91.202	341	6	3	1	324	1	334	0.0	629

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0651450	sp\|Q9LM46\|AG104_ARATH	51.084	323	124	7	1	298	1	314	1.47e-93	284

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0651450	A0A072UYG7	91.202	341	6	3	1	324	1	334	0.0	629

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TFs/TRs:

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Gene ID	Type	Classification
Msa0651450	TF	MADS-MIKC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0651450	MtrunA17_Chr3g0134451	37.175	269	133	5	1	245	1	257	5.22e-46	158
Msa0651450	MtrunA17_Chr4g0044071	100.000	62	0	0	1	62	1	62	3.20e-37	129
Msa0651450	MtrunA17_Chr5g0425191	93.548	62	4	0	1	62	1	62	8.37e-36	125
Msa0651450	MtrunA17_Chr5g0425181	52.555	137	15	3	128	214	15	151	4.05e-34	123
Msa0651450	MtrunA17_Chr5g0425171	73.418	79	16	3	251	324	1	79	1.69e-25	97.4
Msa0651450	MtrunA17_Chr4g0060841	35.393	178	100	5	1	177	1	164	8.76e-24	100
Msa0651450	MtrunA17_Chr7g0246381	67.241	58	19	0	1	58	1	58	6.52e-23	90.5
Msa0651450	MtrunA17_Chr3g0077501	34.254	181	80	2	1	181	1	142	7.74e-23	94.7
Msa0651450	MtrunA17_Chr3g0097101	34.021	194	88	4	1	194	16	169	9.44e-23	95.1
Msa0651450	MtrunA17_Chr5g0417661	66.129	62	21	0	1	62	56	117	5.03e-22	89.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0651450	AT1G22130.1	51.084	323	124	7	1	298	1	314	1.49e-94	284
Msa0651450	AT1G77980.1	46.802	344	127	6	1	300	1	332	1.99e-90	273
Msa0651450	AT1G77950.3	59.563	183	72	1	1	181	1	183	2.70e-72	224
Msa0651450	AT1G77950.2	59.563	183	72	1	1	181	1	183	2.70e-72	224
Msa0651450	AT1G77950.1	59.563	183	72	1	1	181	1	183	2.70e-72	224
Msa0651450	AT1G77950.4	59.563	183	72	1	1	181	1	183	2.70e-72	224
Msa0651450	AT1G18750.4	44.681	141	64	5	1	140	1	128	8.37e-26	106
Msa0651450	AT1G18750.1	44.681	141	64	5	1	140	1	128	1.05e-25	106
Msa0651450	AT2G03060.3	35.165	182	103	4	1	181	1	168	8.73e-25	103
Msa0651450	AT2G03060.7	35.165	182	103	4	1	181	1	168	8.73e-25	103

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Find 57 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAACCATATACAGTAG+AGG	0.670428	4_3:-58831772	None:intergenic
AAATGATTAAGACGATTTGA+TGG	0.363568	4_3:-58828247	None:intergenic
AAATGGTAGAGGAGCAAATA+AGG	0.337725	4_3:+58829888	Msa0651450:CDS
AACATTCAAGCGTGGCCTCA+AGG	0.529883	4_3:+58831870	Msa0651450:CDS
AATGTTAGTTGGCTGCAAGA+TGG	0.658329	4_3:+58831497	Msa0651450:CDS
ACAACAACTTCAAATGGTAG+AGG	0.593286	4_3:+58829877	Msa0651450:CDS
ACATATTCAAAACGTAGAAA+TGG	0.460629	4_3:+58828157	Msa0651450:CDS
ACATATTCAACATGACATTG+TGG	0.591900	4_3:+58831920	Msa0651450:CDS
ACGATGGAAACATTCAAGCG+TGG	0.690768	4_3:+58831862	Msa0651450:CDS
AGAAACCTTGGCACGTGTCA+TGG	0.475032	4_3:+58830088	Msa0651450:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGCAATATCAATATCACAA+AGG	-	chr4_3:58828213-58828232	None:intergenic	30.0%
GAACATACTTATCTCTTTCT+TGG	-	chr4_3:58828848-58828867	None:intergenic	30.0%
AGCAGTAGATGAAACAATAT+AGG	-	chr4_3:58829655-58829674	None:intergenic	30.0%
GTAATCTACTAACTTCTTGT+TGG	-	chr4_3:58829856-58829875	None:intergenic	30.0%
GCTATAGATGTCATCTTTAA+TGG	-	chr4_3:58830038-58830057	None:intergenic	30.0%
AAAAAACGTAGTAGAAACCT+TGG	+	chr4_3:58830076-58830095	Msa0651450:CDS	30.0%
TTAATTACCTGAATAGCTGA+TGG	-	chr4_3:58830286-58830305	None:intergenic	30.0%
TGTCACTCAAAATTGAGATT+TGG	+	chr4_3:58830327-58830346	Msa0651450:intron	30.0%
GAACCTTATATGTCAAAATC+GGG	-	chr4_3:58830449-58830468	None:intergenic	30.0%
CGAACCTTATATGTCAAAAT+CGG	-	chr4_3:58830450-58830469	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	58828100	58832097	58828100	ID=Msa0651450;Name=Msa0651450
chr4_3	mRNA	58828100	58832097	58828100	ID=Msa0651450-mRNA-1;Parent=Msa0651450;Name=Msa0651450-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|0.87\|0.88\|9\|0\|324
chr4_3	exon	58828100	58828284	58828100	ID=Msa0651450-mRNA-1:exon:15648;Parent=Msa0651450-mRNA-1
chr4_3	exon	58828806	58828859	58828806	ID=Msa0651450-mRNA-1:exon:15649;Parent=Msa0651450-mRNA-1
chr4_3	exon	58828973	58829005	58828973	ID=Msa0651450-mRNA-1:exon:15650;Parent=Msa0651450-mRNA-1
chr4_3	exon	58829469	58829549	58829469	ID=Msa0651450-mRNA-1:exon:15651;Parent=Msa0651450-mRNA-1
chr4_3	exon	58829684	58829711	58829684	ID=Msa0651450-mRNA-1:exon:15652;Parent=Msa0651450-mRNA-1
chr4_3	exon	58829848	58829909	58829848	ID=Msa0651450-mRNA-1:exon:15653;Parent=Msa0651450-mRNA-1
chr4_3	exon	58830019	58830118	58830019	ID=Msa0651450-mRNA-1:exon:15654;Parent=Msa0651450-mRNA-1
chr4_3	exon	58831476	58831573	58831476	ID=Msa0651450-mRNA-1:exon:15655;Parent=Msa0651450-mRNA-1
chr4_3	exon	58831764	58832097	58831764	ID=Msa0651450-mRNA-1:exon:15656;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58828100	58828284	58828100	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58828806	58828859	58828806	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58828973	58829005	58828973	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58829469	58829549	58829469	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58829684	58829711	58829684	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58829848	58829909	58829848	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58830019	58830118	58830019	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58831476	58831573	58831476	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1
chr4_3	CDS	58831764	58832097	58831764	ID=Msa0651450-mRNA-1:cds;Parent=Msa0651450-mRNA-1

Gene Sequence

>Msa0651450

ATGGGTCGTGTTAAACTTGAGATAAAAAGAATAGAGAACACAGTGAATCGACAAGTTACATATTCAAAACGTAGAAATGGTCTCATTAAGAAAGCTTATGAGTTATCAATCCTTTGTGATATTGATATTGCTCTTATAATGTTTTCTCCATCAAATCGTCTTAATCATTTTTCAGGTAAAAGAAGGATAGAGGATGTTTTAACTCGTTACATTAATATTCCTGACCAAGAAAGAGATAATGCTGTAAGTTTTCCTGAGCTACCCTACCGAAGTTTATATTGCATTTTGCAGTATTTGCTCAGGATACTTCAGCAGTTGAGGAATGAAAATGACATAGCCCTTCATATGTCCAATTCGGGAGACATTAACTCTGAGGTTGAGGATCTCCAACAAGAAGTTAGTAGATTACAACAACAACTTCAAATGGTAGAGGAGCAAATAAGGAAATATGAACCCGACCCATTAAAGATGACATCTATAGCAGAGCTTGAAACCGGTGAAAAAAACGTAGTAGAAACCTTGGCACGTGTCATGGAGAGAAAGGGGATGCCGACCTCTTTCGAGAATGTTAGTTGGCTGCAAGATGGTGGTCAGAACCAACCCCAAATTTTTGATGCATCAGCTCCTATGGATCCTCTCAGGGATTTGTCCTCTACTGTATATGGTTCATTTTCACAAGGGACGAGTTCAAATGTTGTTGATCCACGAGGTATAGGAGAGTGCCACGTGGCAAATCCAAACGATGGAAACATTCAAGCGTGGCCTCAAGGATACACATTATATCCTCAACATCAGCAACATATTCAACATGACATTGTGGGAACTGATCATCATCATCATCACAACATCCAAGATATGATGCTACATGGACAAATGAACATGCCAATCACTGCCTCACAAGTTGAACCTCCCAAAAATGATTCAACAGAATATGATCAATGTAACCAACCTAATCACCAGCTTCATGGTCAATGA

Protein sequence

>Msa0651450

MGRVKLEIKRIENTVNRQVTYSKRRNGLIKKAYELSILCDIDIALIMFSPSNRLNHFSGKRRIEDVLTRYINIPDQERDNAVSFPELPYRSLYCILQYLLRILQQLRNENDIALHMSNSGDINSEVEDLQQEVSRLQQQLQMVEEQIRKYEPDPLKMTSIAELETGEKNVVETLARVMERKGMPTSFENVSWLQDGGQNQPQIFDASAPMDPLRDLSSTVYGSFSQGTSSNVVDPRGIGECHVANPNDGNIQAWPQGYTLYPQHQQHIQHDIVGTDHHHHHNIQDMMLHGQMNMPITASQVEPPKNDSTEYDQCNQPNHQLHGQ*