AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0656030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0656030 MtrunA17_Chr4g0026311 93.571 140 3 1 1 134 1 140 5.89e-89 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0656030 AT2G27470.1 69.231 91 28 0 4 94 8 98 2.67e-41 138
Msa0656030 AT3G53340.5 33.051 118 69 4 7 124 34 141 4.64e-11 57.8
Msa0656030 AT3G53340.1 33.051 118 69 4 7 124 34 141 4.64e-11 57.8

Find 78 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAATTGGGATTGGAATT+AGG 0.112081 4_3:+65071169 None:intergenic
ACATAAAACTTTAGGTTATT+TGG 0.132011 4_3:-65068397 Msa0656030:three_prime_UTR
AATCGGGATAAGAGGAAATT+AGG 0.171009 4_3:+65071145 None:intergenic
TTGAATTGGGATTGGAATTA+GGG 0.198515 4_3:+65071170 None:intergenic
TTCTCATCTCCCTTTCCTTT+TGG 0.216918 4_3:+65068534 None:intergenic
TTTATTTGTATTGTCGCTAA+TGG 0.236400 4_3:+65071194 None:intergenic
CACACGGCGTACGATAGTTT+TGG 0.288672 4_3:+65071074 None:intergenic
GAGCCAAGACATAAAACTTT+AGG 0.307772 4_3:-65068405 Msa0656030:three_prime_UTR
ATCATTTGACTGCAAGGAAT+TGG 0.321482 4_3:+65068096 None:intergenic
GAGGAAATTAGGGTTTGAAT+TGG 0.337086 4_3:+65071156 None:intergenic
TGAGCAGTTCTTTGTGAATT+CGG 0.342639 4_3:-65068214 Msa0656030:three_prime_UTR
CATGAAGCTAAGTGGTGATA+TGG 0.345097 4_3:-65071230 Msa0656030:five_prime_UTR
TCTCTTCTTCGATTTCTAAT+CGG 0.346103 4_3:+65071128 None:intergenic
CTCTTCTTCGATTTCTAATC+GGG 0.350568 4_3:+65071129 None:intergenic
GATGAAGATTCTGGCACTTT+CGG 0.351884 4_3:+65070978 None:intergenic
TTATTTGTATTGTCGCTAAT+GGG 0.359987 4_3:+65071195 None:intergenic
CTTCCTTCCATCAGGGTTAG+TGG 0.376100 4_3:+65068267 None:intergenic
CCTAAAAGCTTCCTTCCATC+AGG 0.379150 4_3:+65068259 None:intergenic
ATCGGGATAAGAGGAAATTA+GGG 0.382382 4_3:+65071146 None:intergenic
AAGGTAGTGGATGAAGATTC+TGG 0.398524 4_3:+65070969 None:intergenic
ATTGCCCTCACCCATTTCAT+TGG 0.404889 4_3:+65068451 None:intergenic
CAGAGTTTACTGAGTTTGTT+GGG 0.413182 4_3:-65070294 Msa0656030:CDS
ATTAGGGTTTGAATTGGGAT+TGG 0.419772 4_3:+65071162 None:intergenic
AGGAAATTAGGGTTTGAATT+GGG 0.425115 4_3:+65071157 None:intergenic
ATACATAGACTACATGATAA+AGG 0.427355 4_3:+65068043 None:intergenic
TGAATTCGGTTATGAATGTC+TGG 0.427835 4_3:-65068200 Msa0656030:three_prime_UTR
TAGTCTATGTATATTATGTT+TGG 0.434406 4_3:-65068032 Msa0656030:three_prime_UTR
CCTCTAAAAGATTCTCTTGA+AGG 0.437256 4_3:-65070271 Msa0656030:intron
CGATTTCTAATCGGGATAAG+AGG 0.448769 4_3:+65071137 None:intergenic
ACAGAGTTTACTGAGTTTGT+TGG 0.451694 4_3:-65070295 Msa0656030:CDS
GGTGATGATGACCAATGAAA+TGG 0.478392 4_3:-65068462 Msa0656030:exon
AGCGGCAGTGCCAAAAGGAA+AGG 0.481373 4_3:-65068544 Msa0656030:CDS
GCAGGGCTAATGATATATGT+AGG 0.482928 4_3:-65070372 Msa0656030:intron
AACGGCAATGTCGCCGTCGT+CGG 0.485500 4_3:+65071026 None:intergenic
ATCGACAATGGCTGAGGCGG+AGG 0.486553 4_3:-65071103 Msa0656030:exon
TATCGTACGCCGTGTGGTGA+AGG 0.496035 4_3:-65071067 Msa0656030:CDS
CTTACGTAGCAGAAAGGTAG+TGG 0.498108 4_3:+65070956 None:intergenic
GTCGCCGTCGTCGGAGCAAG+TGG 0.510785 4_3:+65071035 None:intergenic
GAGTTTAGGAAGAAGAATGC+TGG 0.512368 4_3:-65068573 Msa0656030:CDS
TCAAATTTGTTGCAGCATTG+AGG 0.516386 4_3:+65068169 None:intergenic
TACATAGTTAACTAGTACGA+AGG 0.516538 4_3:+65068343 None:intergenic
ATGTATATTATGTTTGGATG+AGG 0.517268 4_3:-65068026 Msa0656030:three_prime_UTR
GAGAAAATCGACAATGGCTG+AGG 0.540596 4_3:-65071109 Msa0656030:exon
TGAGAAGGGAAAGAAAAGAA+AGG 0.540607 4_3:-65068517 Msa0656030:CDS
AGTTTAGGAAGAAGAATGCT+GGG 0.542985 4_3:-65068572 Msa0656030:CDS
CCGTCTGACAAAGGCGATGG+TGG 0.546278 4_3:-65068483 Msa0656030:CDS
AAAAGGAAAGGGAGATGAGA+AGG 0.549945 4_3:-65068532 Msa0656030:CDS
CAGGGCTAATGATATATGTA+GGG 0.556080 4_3:-65070371 Msa0656030:CDS
ATACATGATTCCTTGTCACT+TGG 0.559234 4_3:-65068369 Msa0656030:three_prime_UTR
CTAAAAGCTTCCTTCCATCA+GGG 0.559834 4_3:+65068260 None:intergenic
AAGAAAGCGGCAGTGCCAAA+AGG 0.559929 4_3:-65068549 Msa0656030:CDS
GCGGCAGTGCCAAAAGGAAA+GGG 0.560436 4_3:-65068543 Msa0656030:CDS
AAAATCGACAATGGCTGAGG+CGG 0.566675 4_3:-65071106 Msa0656030:exon
CATAACATCATTTGACTGCA+AGG 0.567923 4_3:+65068090 None:intergenic
AAGCTAAGTGGTGATATGGC+GGG 0.577888 4_3:-65071226 Msa0656030:five_prime_UTR
CCACCATCGCCTTTGTCAGA+CGG 0.579482 4_3:+65068483 None:intergenic
CTAGCCTCCACTAACCCTGA+TGG 0.579508 4_3:-65068274 Msa0656030:three_prime_UTR
CTTTCCACTTGCTCCGACGA+CGG 0.580800 4_3:-65071039 Msa0656030:CDS
GTGATGATGACCAATGAAAT+GGG 0.586636 4_3:-65068461 Msa0656030:exon
AAAGGAAAGGGAGATGAGAA+GGG 0.589156 4_3:-65068531 Msa0656030:CDS
AAAGCACTTACGTAGCAGAA+AGG 0.591048 4_3:+65070950 None:intergenic
GAAGGCGAGCCGTCTGACAA+AGG 0.603385 4_3:-65068492 Msa0656030:CDS
CAAAACTATCGTACGCCGTG+TGG 0.603664 4_3:-65071073 Msa0656030:CDS
GGAAAGAAAAGAAAGGCGGA+AGG 0.603937 4_3:-65068510 Msa0656030:CDS
GAAGCTAAGTGGTGATATGG+CGG 0.605561 4_3:-65071227 Msa0656030:five_prime_UTR
CCTTCCATCAGGGTTAGTGG+AGG 0.606434 4_3:+65068270 None:intergenic
GAGCCGTCTGACAAAGGCGA+TGG 0.609316 4_3:-65068486 Msa0656030:CDS
GAAGGGAAAGAAAAGAAAGG+CGG 0.609576 4_3:-65068514 Msa0656030:CDS
AAGAAGAGCGTCTTTGTGAA+CGG 0.610289 4_3:+65071008 None:intergenic
GATGACCAATGAAATGGGTG+AGG 0.612548 4_3:-65068456 Msa0656030:exon
CCTCCACTAACCCTGATGGA+AGG 0.617464 4_3:-65068270 Msa0656030:three_prime_UTR
GAAGAAGAGAAAATCGACAA+TGG 0.639571 4_3:-65071115 Msa0656030:exon
GAAGAATGCTGGGAAGAAAG+CGG 0.668115 4_3:-65068562 Msa0656030:CDS
ATGACCAATGAAATGGGTGA+GGG 0.673171 4_3:-65068455 Msa0656030:exon
ATATATGTAGGGAGTCAAAG+AGG 0.682246 4_3:-65070360 Msa0656030:CDS
ACGAAGGCATCCAAGTGACA+AGG 0.689900 4_3:+65068359 None:intergenic
AAAGCTTCTCCTTCACCACA+CGG 0.706469 4_3:+65071058 None:intergenic
TTGAGTGTATGTAGTAAACG+TGG 0.725186 4_3:-65068141 Msa0656030:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTGTTTTATCAAAA+AGG + chr4_3:65070127-65070146 None:intergenic 10.0%
!! ACATCAAATTTATCAAATTA+GGG + chr4_3:65068379-65068398 None:intergenic 15.0%
!!! TAATTGAGGAAATTTTTTAA+TGG - chr4_3:65068442-65068461 Msa0656030:three_prime_UTR 15.0%
!!! AATTGAGGAAATTTTTTAAT+GGG - chr4_3:65068443-65068462 Msa0656030:three_prime_UTR 15.0%
!!! AAAAATTAGGGTTTATATTT+TGG - chr4_3:65068744-65068763 Msa0656030:intron 15.0%
!! AACAAAATTAAACTAACAAA+AGG + chr4_3:65069075-65069094 None:intergenic 15.0%
!! GACATCAAATTTATCAAATT+AGG + chr4_3:65068380-65068399 None:intergenic 20.0%
!!! TTTGTATTTTGGAGAAAATT+GGG - chr4_3:65068536-65068555 Msa0656030:CDS 20.0%
!!! AATTGGTGAAATTTTGTTAT+GGG - chr4_3:65068658-65068677 Msa0656030:intron 20.0%
!!! TTTTTTAGCTATTGATTCTT+GGG - chr4_3:65068681-65068700 Msa0656030:intron 20.0%
!! TAAGAAAGGAGATAAAAATT+AGG - chr4_3:65068731-65068750 Msa0656030:intron 20.0%
!! AAGAAAGGAGATAAAAATTA+GGG - chr4_3:65068732-65068751 Msa0656030:intron 20.0%
!!! TTTATATTTTGGAGGAAATT+GGG - chr4_3:65068755-65068774 Msa0656030:intron 20.0%
!!! TGTCATTTATATATCTGTTA+TGG - chr4_3:65069151-65069170 Msa0656030:intron 20.0%
!!! ACAAAAGTACTTAAATAAAG+AGG + chr4_3:65069769-65069788 None:intergenic 20.0%
!! ATAAAGAAAAACTAAAACTG+TGG + chr4_3:65070056-65070075 None:intergenic 20.0%
!!! CAGTTTTAGTTTTTCTTTAT+CGG - chr4_3:65070056-65070075 Msa0656030:intron 20.0%
!!! AAAAAAAAAAGTGTTAAGGT+CGG + chr4_3:65070650-65070669 None:intergenic 20.0%
!!! GTCAAAAAAAAAAAGTGTTA+AGG + chr4_3:65070654-65070673 None:intergenic 20.0%
!!! ACATAAAACTTTAGGTTATT+TGG - chr4_3:65070856-65070875 Msa0656030:intron 20.0%
!! TAGTCTATGTATATTATGTT+TGG - chr4_3:65071221-65071240 Msa0656030:five_prime_UTR 20.0%
! TTATTTGTATTGTCGCTAAT+GGG + chr4_3:65068061-65068080 None:intergenic 25.0%
! TTTATTTGTATTGTCGCTAA+TGG + chr4_3:65068062-65068081 None:intergenic 25.0%
!! TTAGATTTTGGATGTAATTG+AGG - chr4_3:65068428-65068447 Msa0656030:three_prime_UTR 25.0%
!!! GGAAATTTTTTAATGGGTTT+TGG - chr4_3:65068449-65068468 Msa0656030:exon 25.0%
!! GATTCTAAAAACTGAAACTT+TGG - chr4_3:65068488-65068507 Msa0656030:CDS 25.0%
!!! GTTTGTATTTTGGAGAAAAT+TGG - chr4_3:65068535-65068554 Msa0656030:CDS 25.0%
!! AGTATTTTGCAATGGTTATT+AGG - chr4_3:65068559-65068578 Msa0656030:CDS 25.0%
!!! TTCATGGGTGATTTTAAAAA+TGG - chr4_3:65068587-65068606 Msa0656030:intron 25.0%
!!! TCATGGGTGATTTTAAAAAT+GGG - chr4_3:65068588-65068607 Msa0656030:intron 25.0%
!!! TTAAAAATGGGTACTTTTTG+AGG - chr4_3:65068600-65068619 Msa0656030:intron 25.0%
!!! AAGTAGGGTTTATATTTTTG+TGG - chr4_3:65068634-65068653 Msa0656030:intron 25.0%
!!! GTTTATATTTTTGTGGCAAT+TGG - chr4_3:65068641-65068660 Msa0656030:intron 25.0%
!! CAATTGGTGAAATTTTGTTA+TGG - chr4_3:65068657-65068676 Msa0656030:intron 25.0%
!!! GTTTTTTAGCTATTGATTCT+TGG - chr4_3:65068680-65068699 Msa0656030:intron 25.0%
!!! TTCTTGGGTGATTTTAAAAA+TGG - chr4_3:65068696-65068715 Msa0656030:intron 25.0%
!!! TCTTGGGTGATTTTAAAAAT+GGG - chr4_3:65068697-65068716 Msa0656030:intron 25.0%
!!! AATTAGGGTTTATATTTTGG+AGG - chr4_3:65068747-65068766 Msa0656030:intron 25.0%
!!! GTTTATATTTTGGAGGAAAT+TGG - chr4_3:65068754-65068773 Msa0656030:intron 25.0%
!!! TTATATTTTGGAGGAAATTG+GGG - chr4_3:65068756-65068775 Msa0656030:intron 25.0%
! TTTGTGGGTGATTATAAAAA+TGG - chr4_3:65068806-65068825 Msa0656030:intron 25.0%
! TTGTGGGTGATTATAAAAAT+GGG - chr4_3:65068807-65068826 Msa0656030:intron 25.0%
!!! TAAAAATGGGAACTTTTTTG+AGG - chr4_3:65068820-65068839 Msa0656030:intron 25.0%
! TAAAGTCATATCAAAGGTAA+GGG + chr4_3:65069015-65069034 None:intergenic 25.0%
!!! GTTAGTTTAATTTTGTTAGC+TGG - chr4_3:65069078-65069097 Msa0656030:intron 25.0%
!! TATGGTTTTCTTCTTTTCAT+TGG - chr4_3:65069169-65069188 Msa0656030:intron 25.0%
! TTGGATAGAAGTTCAAAAAA+TGG - chr4_3:65069188-65069207 Msa0656030:intron 25.0%
! TAATCCCCATTTAGTTAAAA+AGG - chr4_3:65069242-65069261 Msa0656030:intron 25.0%
! AATCCCCATTTAGTTAAAAA+GGG - chr4_3:65069243-65069262 Msa0656030:intron 25.0%
! TATCATTCTTGGTGAAAATT+AGG - chr4_3:65069271-65069290 Msa0656030:intron 25.0%
!!! TTAAGTACTTTTGTTTACAG+AGG - chr4_3:65069775-65069794 Msa0656030:intron 25.0%
!!! TAACCTAAAGTTTTATGTCT+TGG + chr4_3:65070854-65070873 None:intergenic 25.0%
!!! CTTTTAGGAAAGAATATGAA+GGG - chr4_3:65071009-65071028 Msa0656030:CDS 25.0%
! ATACATAGACTACATGATAA+AGG + chr4_3:65071213-65071232 None:intergenic 25.0%
! ATGTATATTATGTTTGGATG+AGG - chr4_3:65071227-65071246 Msa0656030:five_prime_UTR 25.0%
! GAAATTTTCATGAAGCTAAG+TGG - chr4_3:65068015-65068034 Msa0656030:three_prime_UTR 30.0%
TTGAATTGGGATTGGAATTA+GGG + chr4_3:65068086-65068105 None:intergenic 30.0%
TTTGAATTGGGATTGGAATT+AGG + chr4_3:65068087-65068106 None:intergenic 30.0%
AGGAAATTAGGGTTTGAATT+GGG + chr4_3:65068099-65068118 None:intergenic 30.0%
TCTCTTCTTCGATTTCTAAT+CGG + chr4_3:65068128-65068147 None:intergenic 30.0%
CAAATTAGAGTTTAGAGAGA+AGG + chr4_3:65068331-65068350 None:intergenic 30.0%
! AAATCCCCTTTTTCTTTAAG+TGG - chr4_3:65068402-65068421 Msa0656030:three_prime_UTR 30.0%
! AATCCCCTTTTTCTTTAAGT+GGG - chr4_3:65068403-65068422 Msa0656030:three_prime_UTR 30.0%
AAACCCCACTTAAAGAAAAA+GGG + chr4_3:65068410-65068429 None:intergenic 30.0%
TAAACCCCACTTAAAGAAAA+AGG + chr4_3:65068411-65068430 None:intergenic 30.0%
! TTTAAGTGGGGTTTAGATTT+TGG - chr4_3:65068416-65068435 Msa0656030:three_prime_UTR 30.0%
!! AAACTGAAACTTTGGTCTTA+AGG - chr4_3:65068496-65068515 Msa0656030:CDS 30.0%
! GAAAATTGGGGTTTGTATTT+TGG - chr4_3:65068525-65068544 Msa0656030:CDS 30.0%
!!! AAATTGGGAGTATTTTGCAA+TGG - chr4_3:65068551-65068570 Msa0656030:CDS 30.0%
! TGGTTATTAGGATTGATTCA+TGG - chr4_3:65068571-65068590 Msa0656030:CDS 30.0%
! GGTTATTAGGATTGATTCAT+GGG - chr4_3:65068572-65068591 Msa0656030:CDS 30.0%
! AAATTGGGGAATTTTGCAAT+GGG - chr4_3:65068770-65068789 Msa0656030:intron 30.0%
!!! GGTTTTGACTATTGATTTGT+GGG - chr4_3:65068791-65068810 Msa0656030:intron 30.0%
GTAAAGTCATATCAAAGGTA+AGG + chr4_3:65069016-65069035 None:intergenic 30.0%
ACTCAGTAAAGTCATATCAA+AGG + chr4_3:65069021-65069040 None:intergenic 30.0%
!!! TGTTTTAAGACTTAGCATCA+CGG - chr4_3:65069121-65069140 Msa0656030:intron 30.0%
!! CAACCCTTTTTAACTAAATG+GGG + chr4_3:65069249-65069268 None:intergenic 30.0%
!! CCAACCCTTTTTAACTAAAT+GGG + chr4_3:65069250-65069269 None:intergenic 30.0%
!! ACCAACCCTTTTTAACTAAA+TGG + chr4_3:65069251-65069270 None:intergenic 30.0%
!!! CATTTTAGAGTCATAGTTCA+TGG + chr4_3:65069311-65069330 None:intergenic 30.0%
!! CACTTTTAGGCTTTTAACTA+TGG + chr4_3:65069338-65069357 None:intergenic 30.0%
!! GCATAGAATAACTCACTTTT+AGG + chr4_3:65069351-65069370 None:intergenic 30.0%
TTATGCTACATAAACAAGTG+TGG - chr4_3:65069445-65069464 Msa0656030:intron 30.0%
!! AAACTATTATCGAGTTGGTT+CGG + chr4_3:65069491-65069510 None:intergenic 30.0%
! GACAATTGACTCATTGAATT+TGG - chr4_3:65069523-65069542 Msa0656030:intron 30.0%
GAGGAAGCTTGTAATAAATT+AGG + chr4_3:65069750-65069769 None:intergenic 30.0%
TGAGTTTAAGAACATTAGAG+AGG - chr4_3:65069921-65069940 Msa0656030:intron 30.0%
!!! GCTACTACTTTTTTCTTTAG+TGG - chr4_3:65070012-65070031 Msa0656030:intron 30.0%
! TTTAGAGTTCATGAACAACT+CGG + chr4_3:65070203-65070222 None:intergenic 30.0%
CCAAAAAAACATATCGAGTT+TGG + chr4_3:65070230-65070249 None:intergenic 30.0%
!!! CCAAACTCGATATGTTTTTT+TGG - chr4_3:65070227-65070246 Msa0656030:intron 30.0%
TCGAACTCGAGTTAAAAAAA+TGG - chr4_3:65070330-65070349 Msa0656030:CDS 30.0%
AAGAGGAATGAAACTATTTG+TGG - chr4_3:65070485-65070504 Msa0656030:intron 30.0%
AGAGGAATGAAACTATTTGT+GGG - chr4_3:65070486-65070505 Msa0656030:intron 30.0%
AAGAGATATGTGTAGTTGTT+TGG - chr4_3:65070597-65070616 Msa0656030:intron 30.0%
! TTTTTGACCTATCAGAGTTT+AGG - chr4_3:65070666-65070685 Msa0656030:intron 30.0%
TACATAGTTAACTAGTACGA+AGG + chr4_3:65070913-65070932 None:intergenic 30.0%
! AGCGTTTTACATACAATACT+TGG + chr4_3:65070961-65070980 None:intergenic 30.0%
!! GCTTTTAGGAAAGAATATGA+AGG - chr4_3:65071008-65071027 Msa0656030:CDS 30.0%
ATTAGGGTTTGAATTGGGAT+TGG + chr4_3:65068094-65068113 None:intergenic 35.0%
GAGGAAATTAGGGTTTGAAT+TGG + chr4_3:65068100-65068119 None:intergenic 35.0%
ATCGGGATAAGAGGAAATTA+GGG + chr4_3:65068110-65068129 None:intergenic 35.0%
AATCGGGATAAGAGGAAATT+AGG + chr4_3:65068111-65068130 None:intergenic 35.0%
CTCTTCTTCGATTTCTAATC+GGG + chr4_3:65068127-65068146 None:intergenic 35.0%
GAAGAAGAGAAAATCGACAA+TGG - chr4_3:65068138-65068157 Msa0656030:three_prime_UTR 35.0%
! ATCCCCTTTTTCTTTAAGTG+GGG - chr4_3:65068404-65068423 Msa0656030:three_prime_UTR 35.0%
AACCCCACTTAAAGAAAAAG+GGG + chr4_3:65068409-65068428 None:intergenic 35.0%
!! GGTTTTGGTGATTGATTCAT+GGG - chr4_3:65068464-65068483 Msa0656030:exon 35.0%
!! TGAAACTTTGGTCTTAAGGA+AGG - chr4_3:65068500-65068519 Msa0656030:CDS 35.0%
TCTTAAGGAAGGAGGAAAAT+TGG - chr4_3:65068511-65068530 Msa0656030:CDS 35.0%
CTTAAGGAAGGAGGAAAATT+GGG - chr4_3:65068512-65068531 Msa0656030:CDS 35.0%
TTAAGGAAGGAGGAAAATTG+GGG - chr4_3:65068513-65068532 Msa0656030:CDS 35.0%
!! AAATGGGTACTTTTTGAGGA+AGG - chr4_3:65068604-65068623 Msa0656030:intron 35.0%
TGAGGAAGGAGATAAAAAGT+AGG - chr4_3:65068618-65068637 Msa0656030:intron 35.0%
GAGGAAGGAGATAAAAAGTA+GGG - chr4_3:65068619-65068638 Msa0656030:intron 35.0%
! GGGAACTTTGTTTGTAAGAA+AGG - chr4_3:65068717-65068736 Msa0656030:intron 35.0%
! GAAATTGGGGAATTTTGCAA+TGG - chr4_3:65068769-65068788 Msa0656030:intron 35.0%
!!! GGGTTTTGACTATTGATTTG+TGG - chr4_3:65068790-65068809 Msa0656030:intron 35.0%
!! TTGGTGTTGTTTGTATTTGC+AGG - chr4_3:65068863-65068882 Msa0656030:intron 35.0%
!! TGGTGTTGTTTGTATTTGCA+GGG - chr4_3:65068864-65068883 Msa0656030:intron 35.0%
CAGGGCTAATGATATATGTA+GGG - chr4_3:65068882-65068901 Msa0656030:intron 35.0%
ATATATGTAGGGAGTCAAAG+AGG - chr4_3:65068893-65068912 Msa0656030:intron 35.0%
ACAGAGTTTACTGAGTTTGT+TGG - chr4_3:65068958-65068977 Msa0656030:intron 35.0%
! CAGAGTTTACTGAGTTTGTT+GGG - chr4_3:65068959-65068978 Msa0656030:intron 35.0%
! CCTTCAAGAGAATCTTTTAG+AGG + chr4_3:65068985-65069004 None:intergenic 35.0%
! CCTCTAAAAGATTCTCTTGA+AGG - chr4_3:65068982-65069001 Msa0656030:intron 35.0%
CCCATTTAGTTAAAAAGGGT+TGG - chr4_3:65069247-65069266 Msa0656030:intron 35.0%
!! AAAGGGTTGGTTATCATTCT+TGG - chr4_3:65069260-65069279 Msa0656030:intron 35.0%
CATTCTTGGTGAAAATTAGG+AGG - chr4_3:65069274-65069293 Msa0656030:intron 35.0%
ATGGAAGATATGATCTTGCT+TGG - chr4_3:65069406-65069425 Msa0656030:intron 35.0%
ATAAACAAGTGTGGTCAGTA+GGG - chr4_3:65069454-65069473 Msa0656030:intron 35.0%
GAACCAACTCGATAATAGTT+TGG - chr4_3:65069490-65069509 Msa0656030:intron 35.0%
GTTCCAAACTATTATCGAGT+TGG + chr4_3:65069496-65069515 None:intergenic 35.0%
GAGTTGTATATAGTTCGACT+CGG - chr4_3:65069611-65069630 Msa0656030:intron 35.0%
GGGACTAAGTTATACATGTT+TGG + chr4_3:65069894-65069913 None:intergenic 35.0%
TGTATAACTTAGTCCCACAT+CGG - chr4_3:65069898-65069917 Msa0656030:intron 35.0%
GTTTAAGAACATTAGAGAGG+AGG - chr4_3:65069924-65069943 Msa0656030:intron 35.0%
AAAAGAGAAGCTTGTGTGTA+CGG - chr4_3:65069956-65069975 Msa0656030:intron 35.0%
GCACTGCAGTAGTAATTATA+AGG - chr4_3:65069987-65070006 Msa0656030:intron 35.0%
ATTAAAGTTCGCGAACAACT+CGG + chr4_3:65070306-65070325 None:intergenic 35.0%
GTCAGCTAGTGAGTAATATT+TGG - chr4_3:65070451-65070470 Msa0656030:intron 35.0%
GCTAGTGAGTAATATTTGGT+TGG - chr4_3:65070455-65070474 Msa0656030:intron 35.0%
TCTTCTTCCTAAACTCTGAT+AGG + chr4_3:65070676-65070695 None:intergenic 35.0%
AGTTTAGGAAGAAGAATGCT+GGG - chr4_3:65070681-65070700 Msa0656030:intron 35.0%
TGAGAAGGGAAAGAAAAGAA+AGG - chr4_3:65070736-65070755 Msa0656030:intron 35.0%
! GTGATGATGACCAATGAAAT+GGG - chr4_3:65070792-65070811 Msa0656030:intron 35.0%
GAGCCAAGACATAAAACTTT+AGG - chr4_3:65070848-65070867 Msa0656030:intron 35.0%
ATACATGATTCCTTGTCACT+TGG - chr4_3:65070884-65070903 Msa0656030:intron 35.0%
TGAGCAGTTCTTTGTGAATT+CGG - chr4_3:65071039-65071058 Msa0656030:CDS 35.0%
TGAATTCGGTTATGAATGTC+TGG - chr4_3:65071053-65071072 Msa0656030:CDS 35.0%
!! TCAAATTTGTTGCAGCATTG+AGG + chr4_3:65071087-65071106 None:intergenic 35.0%
TTGAGTGTATGTAGTAAACG+TGG - chr4_3:65071112-65071131 Msa0656030:exon 35.0%
ATCATTTGACTGCAAGGAAT+TGG + chr4_3:65071160-65071179 None:intergenic 35.0%
CATAACATCATTTGACTGCA+AGG + chr4_3:65071166-65071185 None:intergenic 35.0%
CATGAAGCTAAGTGGTGATA+TGG - chr4_3:65068023-65068042 Msa0656030:three_prime_UTR 40.0%
CGATTTCTAATCGGGATAAG+AGG + chr4_3:65068119-65068138 None:intergenic 40.0%
!! AAGAAGAGCGTCTTTGTGAA+CGG + chr4_3:65068248-65068267 None:intergenic 40.0%
GATGAAGATTCTGGCACTTT+CGG + chr4_3:65068278-65068297 None:intergenic 40.0%
!! AAGGTAGTGGATGAAGATTC+TGG + chr4_3:65068287-65068306 None:intergenic 40.0%
! AAAGCACTTACGTAGCAGAA+AGG + chr4_3:65068306-65068325 None:intergenic 40.0%
!! GGGTTTTGGTGATTGATTCA+TGG - chr4_3:65068463-65068482 Msa0656030:exon 40.0%
!! AACTTTGGTCTTAAGGAAGG+AGG - chr4_3:65068503-65068522 Msa0656030:CDS 40.0%
AGAAGAAGCTGAAGAGTGAT+TGG - chr4_3:65068844-65068863 Msa0656030:intron 40.0%
GCAGGGCTAATGATATATGT+AGG - chr4_3:65068881-65068900 Msa0656030:intron 40.0%
TGAACCAACTCTAAGCATGA+TGG - chr4_3:65069387-65069406 Msa0656030:intron 40.0%
TCTTCCATCATGCTTAGAGT+TGG + chr4_3:65069394-65069413 None:intergenic 40.0%
CATAAACAAGTGTGGTCAGT+AGG - chr4_3:65069453-65069472 Msa0656030:intron 40.0%
!! TGTATATAGTTCGACTCGGT+TGG - chr4_3:65069615-65069634 Msa0656030:intron 40.0%
GTTCTTAAACTCACCGATGT+GGG + chr4_3:65069914-65069933 None:intergenic 40.0%
TGTTCTTAAACTCACCGATG+TGG + chr4_3:65069915-65069934 None:intergenic 40.0%
ACTAAAACTGTGGAGCATGT+CGG + chr4_3:65070046-65070065 None:intergenic 40.0%
!! ATTTGGTTGGTGCTACAAAG+AGG - chr4_3:65070468-65070487 Msa0656030:intron 40.0%
GAGTTTAGGAAGAAGAATGC+TGG - chr4_3:65070680-65070699 Msa0656030:intron 40.0%
! TTCTCATCTCCCTTTCCTTT+TGG + chr4_3:65070722-65070741 None:intergenic 40.0%
AAAAGGAAAGGGAGATGAGA+AGG - chr4_3:65070721-65070740 Msa0656030:intron 40.0%
AAAGGAAAGGGAGATGAGAA+GGG - chr4_3:65070722-65070741 Msa0656030:intron 40.0%
GAAGGGAAAGAAAAGAAAGG+CGG - chr4_3:65070739-65070758 Msa0656030:intron 40.0%
! GGTGATGATGACCAATGAAA+TGG - chr4_3:65070791-65070810 Msa0656030:intron 40.0%
ATGACCAATGAAATGGGTGA+GGG - chr4_3:65070798-65070817 Msa0656030:intron 40.0%
CTAAAAGCTTCCTTCCATCA+GGG + chr4_3:65070996-65071015 None:intergenic 40.0%
GAAGCTAAGTGGTGATATGG+CGG - chr4_3:65068026-65068045 Msa0656030:three_prime_UTR 45.0%
AAGCTAAGTGGTGATATGGC+GGG - chr4_3:65068027-65068046 Msa0656030:three_prime_UTR 45.0%
GAGAAAATCGACAATGGCTG+AGG - chr4_3:65068144-65068163 Msa0656030:three_prime_UTR 45.0%
AAAATCGACAATGGCTGAGG+CGG - chr4_3:65068147-65068166 Msa0656030:three_prime_UTR 45.0%
! ACACGGCGTACGATAGTTTT+GGG + chr4_3:65068181-65068200 None:intergenic 45.0%
AAAGCTTCTCCTTCACCACA+CGG + chr4_3:65068198-65068217 None:intergenic 45.0%
CTTACGTAGCAGAAAGGTAG+TGG + chr4_3:65068300-65068319 None:intergenic 45.0%
TGGAGAGTGAAGTGAAGCTT+TGG - chr4_3:65069098-65069117 Msa0656030:intron 45.0%
CGAGCCGAACCAATTCTTAT+CGG - chr4_3:65070378-65070397 Msa0656030:intron 45.0%
GAGCCGAACCAATTCTTATC+GGG - chr4_3:65070379-65070398 Msa0656030:intron 45.0%
CGACCCGATAAGAATTGGTT+CGG + chr4_3:65070385-65070404 None:intergenic 45.0%
TACTCACTAGCTGACCACTA+GGG + chr4_3:65070446-65070465 None:intergenic 45.0%
TTACTCACTAGCTGACCACT+AGG + chr4_3:65070447-65070466 None:intergenic 45.0%
GACCAATATCTGCCCTAACT+TGG - chr4_3:65070624-65070643 Msa0656030:intron 45.0%
TGTTAAGGTCGGCCAAGTTA+GGG + chr4_3:65070639-65070658 None:intergenic 45.0%
GAAGAATGCTGGGAAGAAAG+CGG - chr4_3:65070691-65070710 Msa0656030:intron 45.0%
GGAAAGAAAAGAAAGGCGGA+AGG - chr4_3:65070743-65070762 Msa0656030:intron 45.0%
GATGACCAATGAAATGGGTG+AGG - chr4_3:65070797-65070816 Msa0656030:intron 45.0%
ATTGCCCTCACCCATTTCAT+TGG + chr4_3:65070805-65070824 None:intergenic 45.0%
CCTAAAAGCTTCCTTCCATC+AGG + chr4_3:65070997-65071016 None:intergenic 45.0%
! CCTGATGGAAGGAAGCTTTT+AGG - chr4_3:65070994-65071013 Msa0656030:CDS 45.0%
!! AAATTGTAAAATTTATAATT+AGG - chr4_3:65069217-65069236 Msa0656030:intron 5.0%
!!! TTTTTTTTTAATGAATTAAA+AGG - chr4_3:65070091-65070110 Msa0656030:intron 5.0%
! CACACGGCGTACGATAGTTT+TGG + chr4_3:65068182-65068201 None:intergenic 50.0%
CAAAACTATCGTACGCCGTG+TGG - chr4_3:65068180-65068199 Msa0656030:three_prime_UTR 50.0%
AGGTTCAACTCGTTTGCTCG+AGG + chr4_3:65070169-65070188 None:intergenic 50.0%
! ACAACTCGGTTCGTCTCGAT+AGG + chr4_3:65070189-65070208 None:intergenic 50.0%
ACAACTCGGTTCAGCTCGAT+AGG + chr4_3:65070292-65070311 None:intergenic 50.0%
CGACTCGACCCGATAAGAAT+TGG + chr4_3:65070390-65070409 None:intergenic 50.0%
GGCCAAGTTAGGGCAGATAT+TGG + chr4_3:65070629-65070648 None:intergenic 50.0%
GTGTTAAGGTCGGCCAAGTT+AGG + chr4_3:65070640-65070659 None:intergenic 50.0%
AAGAAAGCGGCAGTGCCAAA+AGG - chr4_3:65070704-65070723 Msa0656030:intron 50.0%
!! ACGAAGGCATCCAAGTGACA+AGG + chr4_3:65070897-65070916 None:intergenic 50.0%
CTTCCTTCCATCAGGGTTAG+TGG + chr4_3:65070989-65071008 None:intergenic 50.0%
TATCGTACGCCGTGTGGTGA+AGG - chr4_3:65068186-65068205 Msa0656030:three_prime_UTR 55.0%
CTTTCCACTTGCTCCGACGA+CGG - chr4_3:65068214-65068233 Msa0656030:three_prime_UTR 55.0%
ACAAGTGTGGTCAGTAGGGC+TGG - chr4_3:65069458-65069477 Msa0656030:intron 55.0%
CGACTCATTTCCAGCCCTAG+TGG - chr4_3:65070429-65070448 Msa0656030:intron 55.0%
AGCGGCAGTGCCAAAAGGAA+AGG - chr4_3:65070709-65070728 Msa0656030:intron 55.0%
GCGGCAGTGCCAAAAGGAAA+GGG - chr4_3:65070710-65070729 Msa0656030:intron 55.0%
CCACCATCGCCTTTGTCAGA+CGG + chr4_3:65070773-65070792 None:intergenic 55.0%
CTAGCCTCCACTAACCCTGA+TGG - chr4_3:65070979-65070998 Msa0656030:CDS 55.0%
! CCTTCCATCAGGGTTAGTGG+AGG + chr4_3:65070986-65071005 None:intergenic 55.0%
CCTCCACTAACCCTGATGGA+AGG - chr4_3:65070983-65071002 Msa0656030:CDS 55.0%
ATCGACAATGGCTGAGGCGG+AGG - chr4_3:65068150-65068169 Msa0656030:three_prime_UTR 60.0%
AACGGCAATGTCGCCGTCGT+CGG + chr4_3:65068230-65068249 None:intergenic 60.0%
CACTAGCTGACCACTAGGGC+TGG + chr4_3:65070442-65070461 None:intergenic 60.0%
GAAGGCGAGCCGTCTGACAA+AGG - chr4_3:65070761-65070780 Msa0656030:intron 60.0%
GAGCCGTCTGACAAAGGCGA+TGG - chr4_3:65070767-65070786 Msa0656030:intron 60.0%
CCGTCTGACAAAGGCGATGG+TGG - chr4_3:65070770-65070789 Msa0656030:intron 60.0%
GTCGCCGTCGTCGGAGCAAG+TGG + chr4_3:65068221-65068240 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 65067997 65071278 65067997 ID=Msa0656030;Name=Msa0656030
chr4_3 mRNA 65067997 65071278 65067997 ID=Msa0656030-mRNA-1;Parent=Msa0656030;Name=Msa0656030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=160|1|1|1|1|1|3|470|134
chr4_3 exon 65067997 65068594 65067997 ID=Msa0656030-mRNA-1:exon:18447;Parent=Msa0656030-mRNA-1
chr4_3 exon 65070272 65070390 65070272 ID=Msa0656030-mRNA-1:exon:18446;Parent=Msa0656030-mRNA-1
chr4_3 exon 65070961 65071278 65070961 ID=Msa0656030-mRNA-1:exon:18445;Parent=Msa0656030-mRNA-1
chr4_3 five_prime_UTR 65071119 65071278 65071119 ID=Msa0656030-mRNA-1:five_prime_utr;Parent=Msa0656030-mRNA-1
chr4_3 CDS 65070961 65071118 65070961 ID=Msa0656030-mRNA-1:cds;Parent=Msa0656030-mRNA-1
chr4_3 CDS 65070272 65070390 65070272 ID=Msa0656030-mRNA-1:cds;Parent=Msa0656030-mRNA-1
chr4_3 CDS 65068467 65068594 65068467 ID=Msa0656030-mRNA-1:cds;Parent=Msa0656030-mRNA-1
chr4_3 three_prime_UTR 65067997 65068466 65067997 ID=Msa0656030-mRNA-1:three_prime_utr;Parent=Msa0656030-mRNA-1
Gene Sequence

>Msa0656030

ATGGCTGAGGCGGAGGAGCTTCCCAAAACTATCGTACGCCGTGTGGTGAAGGAGAAGCTTTCCACTTGCTCCGACGACGGCGACATTGCCGTTCACAAAGACGCTCTTCTTGCATTCTCCGAAAGTGCCAGAATCTTCATCCACTACCTTTCTGCTACGGCTAATGATATATGTAGGGAGTCAAAGAGGCAAATCATCAATGCTGAAGATGTGTTCAAAGCTCTTGAAGAAACAGAGTTTACTGAGTTTGTTGGGCCTCTAAAAGATTCTCTTGAAGAGTTTAGGAAGAAGAATGCTGGGAAGAAAGCGGCAGTGCCAAAAGGAAAGGGAGATGAGAAGGGAAAGAAAAGAAAGGCGGAAGGCGAGCCGTCTGACAAAGGCGATGGTGGTGATGATGACCAATGA
Protein sequence

>Msa0656030

MAEAEELPKTIVRRVVKEKLSTCSDDGDIAVHKDALLAFSESARIFIHYLSATANDICRESKRQIINAEDVFKALEETEFTEFVGPLKDSLEEFRKKNAGKKAAVPKGKGDEKGKKRKAEGEPSDKGDGGDDDQ*