Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0658060 | AET04474.1 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 6.62e-94 | 278 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0658060 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATACCTTCAAATGAAAT+TGG | 0.214941 | 4_3:+68093208 | Msa0658060:CDS |
| CTTTGAGGTTTCCCCAGTTT+TGG | 0.256311 | 4_3:-68092758 | None:intergenic |
| CATAAAACTGGCTTGTCTTC+TGG | 0.266801 | 4_3:-68092714 | None:intergenic |
| ATTCTCAAACTACGTCTCTT+TGG | 0.299791 | 4_3:-68093061 | None:intergenic |
| TCAAACAATAAGGCCTCTTC+TGG | 0.301830 | 4_3:-68093172 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.314815 | 4_3:-68092687 | None:intergenic |
| GATGTTTCTGATTCAGATAA+TGG | 0.318368 | 4_3:+68092873 | Msa0658060:CDS |
| CTGTTCGTAACAAGTGCTTT+TGG | 0.342724 | 4_3:-68092804 | None:intergenic |
| TCTGGTCCCTGGCGCCATGC+TGG | 0.378150 | 4_3:-68092696 | None:intergenic |
| GAAAACAACGTTTGAGAGAA+TGG | 0.387976 | 4_3:-68092782 | None:intergenic |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | 4_3:+68092745 | Msa0658060:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | 4_3:-68092726 | None:intergenic |
| CCTTCAAATGAAATTGGCCT+TGG | 0.419265 | 4_3:+68093214 | Msa0658060:CDS |
| CCAAGGCCAATTTCATTTGA+AGG | 0.421742 | 4_3:-68093214 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.436034 | 4_3:+68092689 | Msa0658060:CDS |
| GTTTGAGAGAATGGTCTTTG+AGG | 0.475222 | 4_3:-68092773 | None:intergenic |
| CTGGCTTGTCTTCTGGTCCC+TGG | 0.494515 | 4_3:-68092707 | None:intergenic |
| TCTTGTTGAAACTTCTCCAT+CGG | 0.530728 | 4_3:-68093103 | None:intergenic |
| ATCTGAATCAGAAACATCAA+TGG | 0.539843 | 4_3:-68092868 | None:intergenic |
| TTCTACATAGAAGAACCGTC+TGG | 0.563851 | 4_3:-68093253 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.585142 | 4_3:+68092690 | Msa0658060:CDS |
| AGATGAGTCATCTCCAGAAG+AGG | 0.602353 | 4_3:+68093159 | Msa0658060:CDS |
| GCGGTTCTTCTTAAACCAGA+CGG | 0.605404 | 4_3:+68093238 | Msa0658060:CDS |
| AAATGAAATTGGCCTTGGAG+CGG | 0.608265 | 4_3:+68093219 | Msa0658060:CDS |
| CAATCAGAAGATGACACCGA+TGG | 0.614119 | 4_3:+68093087 | Msa0658060:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | 4_3:+68092746 | Msa0658060:CDS |
| GTTTAAGAAGAACCGCTCCA+AGG | 0.637380 | 4_3:-68093231 | None:intergenic |
| AAAGCACTTGTTACGAACAG+TGG | 0.651202 | 4_3:+68092807 | Msa0658060:CDS |
| CATCACCAGCAACTCCAGCA+TGG | 0.651498 | 4_3:+68092682 | Msa0658060:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | 4_3:+68092747 | Msa0658060:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATATATATTACTTTTGTG+AGG | + | chr4_3:68092942-68092961 | Msa0658060:intron | 20.0% |
| !!! | GATATATATTACTTTTGTGA+GGG | + | chr4_3:68092943-68092962 | Msa0658060:intron | 20.0% |
| ! | TGTAAAGAAAAGAGTGAAAA+TGG | - | chr4_3:68092921-68092940 | None:intergenic | 25.0% |
| !! | GTTCACATTTTCAAACAATA+AGG | - | chr4_3:68093185-68093204 | None:intergenic | 25.0% |
| ! | TTCATACCTTCAAATGAAAT+TGG | + | chr4_3:68093208-68093227 | Msa0658060:CDS | 25.0% |
| ATCTGAATCAGAAACATCAA+TGG | - | chr4_3:68092871-68092890 | None:intergenic | 30.0% | |
| ! | GATGTTTCTGATTCAGATAA+TGG | + | chr4_3:68092873-68092892 | Msa0658060:CDS | 30.0% |
| !! | ATATATTACTTTTGTGAGGG+TGG | + | chr4_3:68092946-68092965 | Msa0658060:intron | 30.0% |
| !! | TATATTACTTTTGTGAGGGT+GGG | + | chr4_3:68092947-68092966 | Msa0658060:intron | 30.0% |
| TGGACTAAGATACCAAAACT+GGG | + | chr4_3:68092746-68092765 | Msa0658060:CDS | 35.0% | |
| GAAAACAACGTTTGAGAGAA+TGG | - | chr4_3:68092785-68092804 | None:intergenic | 35.0% | |
| ATTCTCAAACTACGTCTCTT+TGG | - | chr4_3:68093064-68093083 | None:intergenic | 35.0% | |
| TCTTGTTGAAACTTCTCCAT+CGG | - | chr4_3:68093106-68093125 | None:intergenic | 35.0% | |
| !! | CATAAAACTGGCTTGTCTTC+TGG | - | chr4_3:68092717-68092736 | None:intergenic | 40.0% |
| CCACATGCATTGCATAAAAC+TGG | - | chr4_3:68092729-68092748 | None:intergenic | 40.0% | |
| !! | CCAGTTTTATGCAATGCATG+TGG | + | chr4_3:68092726-68092745 | Msa0658060:CDS | 40.0% |
| ! | GTGGACTAAGATACCAAAAC+TGG | + | chr4_3:68092745-68092764 | Msa0658060:CDS | 40.0% |
| GGACTAAGATACCAAAACTG+GGG | + | chr4_3:68092747-68092766 | Msa0658060:CDS | 40.0% | |
| GTTTGAGAGAATGGTCTTTG+AGG | - | chr4_3:68092776-68092795 | None:intergenic | 40.0% | |
| ! | CTGTTCGTAACAAGTGCTTT+TGG | - | chr4_3:68092807-68092826 | None:intergenic | 40.0% |
| ! | AAAGCACTTGTTACGAACAG+TGG | + | chr4_3:68092807-68092826 | Msa0658060:CDS | 40.0% |
| TCAAACAATAAGGCCTCTTC+TGG | - | chr4_3:68093175-68093194 | None:intergenic | 40.0% | |
| CCAAGGCCAATTTCATTTGA+AGG | - | chr4_3:68093217-68093236 | None:intergenic | 40.0% | |
| ! | CCTTCAAATGAAATTGGCCT+TGG | + | chr4_3:68093214-68093233 | Msa0658060:CDS | 40.0% |
| !! | AAATGAAATTGGCCTTGGAG+CGG | + | chr4_3:68093219-68093238 | Msa0658060:CDS | 40.0% |
| !!! | CTTTGAGGTTTCCCCAGTTT+TGG | - | chr4_3:68092761-68092780 | None:intergenic | 45.0% |
| CAATCAGAAGATGACACCGA+TGG | + | chr4_3:68093087-68093106 | Msa0658060:CDS | 45.0% | |
| AGATGAGTCATCTCCAGAAG+AGG | + | chr4_3:68093159-68093178 | Msa0658060:CDS | 45.0% | |
| GTTTAAGAAGAACCGCTCCA+AGG | - | chr4_3:68093234-68093253 | None:intergenic | 45.0% | |
| GCGGTTCTTCTTAAACCAGA+CGG | + | chr4_3:68093238-68093257 | Msa0658060:CDS | 45.0% | |
| CATCACCAGCAACTCCAGCA+TGG | + | chr4_3:68092682-68092701 | Msa0658060:CDS | 55.0% | |
| ! | CTGGCTTGTCTTCTGGTCCC+TGG | - | chr4_3:68092710-68092729 | None:intergenic | 60.0% |
| TGGCGCCATGCTGGAGTTGC+TGG | - | chr4_3:68092690-68092709 | None:intergenic | 65.0% | |
| AGCAACTCCAGCATGGCGCC+AGG | + | chr4_3:68092689-68092708 | Msa0658060:CDS | 65.0% | |
| GCAACTCCAGCATGGCGCCA+GGG | + | chr4_3:68092690-68092709 | Msa0658060:CDS | 65.0% | |
| TCTGGTCCCTGGCGCCATGC+TGG | - | chr4_3:68092699-68092718 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 68092675 | 68093273 | 68092675 | ID=Msa0658060;Name=Msa0658060 |
| chr4_3 | mRNA | 68092675 | 68093273 | 68092675 | ID=Msa0658060-mRNA-1;Parent=Msa0658060;Name=Msa0658060-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|156 |
| chr4_3 | exon | 68092675 | 68092894 | 68092675 | ID=Msa0658060-mRNA-1:exon:19666;Parent=Msa0658060-mRNA-1 |
| chr4_3 | exon | 68093023 | 68093273 | 68093023 | ID=Msa0658060-mRNA-1:exon:19667;Parent=Msa0658060-mRNA-1 |
| chr4_3 | CDS | 68092675 | 68092894 | 68092675 | ID=Msa0658060-mRNA-1:cds;Parent=Msa0658060-mRNA-1 |
| chr4_3 | CDS | 68093023 | 68093273 | 68093023 | ID=Msa0658060-mRNA-1:cds;Parent=Msa0658060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
