Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0658070 | AET04474.1 | 88.158 | 152 | 15 | 1 | 4 | 152 | 5 | 156 | 3.91e-85 | 256 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0658070 | G7L9K0 | 88.158 | 152 | 15 | 1 | 4 | 152 | 5 | 156 | 1.87e-85 | 256 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0658070 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAAAACTGGCTTCTCTTC+TGG | 0.255261 | 4_3:-68107848 | None:intergenic |
| TCGAACAATAAGGTCTCTTC+TGG | 0.261617 | 4_3:-68108297 | None:intergenic |
| TTCATACCTTCAAATGAAAT+TGG | 0.274802 | 4_3:+68108333 | Msa0658070:CDS |
| CTTCAAGATTTCCCCAGTTT+TGG | 0.287698 | 4_3:-68107892 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.300530 | 4_3:-68107821 | None:intergenic |
| GATGTTTCTGATTCAGATAA+TGG | 0.318368 | 4_3:+68108007 | Msa0658070:CDS |
| ATCCTCAAACTACGCCTCTT+TGG | 0.326587 | 4_3:-68108195 | None:intergenic |
| CTGTTAGTAAGGAGTTCTTT+TGG | 0.362634 | 4_3:-68107938 | None:intergenic |
| TCTGGTCCCTGGCGCCATGC+TGG | 0.378150 | 4_3:-68107830 | None:intergenic |
| CCAAGACCAATTTCATTTGA+AGG | 0.378872 | 4_3:-68108339 | None:intergenic |
| CCTTCAAATGAAATTGGTCT+TGG | 0.383519 | 4_3:+68108339 | Msa0658070:CDS |
| TACTGTTGCCACTGTTAGTA+AGG | 0.396316 | 4_3:-68107949 | None:intergenic |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | 4_3:+68107879 | Msa0658070:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | 4_3:-68107860 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.413779 | 4_3:+68107823 | Msa0658070:CDS |
| CTCCAAAGAGGCGTAGTTTG+AGG | 0.425603 | 4_3:+68108193 | Msa0658070:CDS |
| CGAACAATAAGGTCTCTTCT+GGG | 0.433397 | 4_3:-68108296 | None:intergenic |
| CTGGCTTCTCTTCTGGTCCC+TGG | 0.474139 | 4_3:-68107841 | None:intergenic |
| TCTTCTGGGGATGACTCATC+TGG | 0.478086 | 4_3:-68108282 | None:intergenic |
| GAGGATTATCAATCAGAAGA+TGG | 0.496715 | 4_3:+68108212 | Msa0658070:CDS |
| TTCATATTCATGCAAGTCTA+CGG | 0.538240 | 4_3:+68108167 | Msa0658070:CDS |
| ATCTGAATCAGAAACATCAA+TGG | 0.539843 | 4_3:-68108002 | None:intergenic |
| TTCTACATAGAAGAACCGTC+TGG | 0.563851 | 4_3:-68108378 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.572723 | 4_3:+68107824 | Msa0658070:CDS |
| TTTGGAAAACAACGTTTGAG+AGG | 0.575726 | 4_3:-68107920 | None:intergenic |
| GCAGTTCTTCTTAAACCAGA+CGG | 0.605128 | 4_3:+68108363 | Msa0658070:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | 4_3:+68107880 | Msa0658070:CDS |
| GAACAATAAGGTCTCTTCTG+GGG | 0.630133 | 4_3:-68108295 | None:intergenic |
| AAAGAACTCCTTACTAACAG+TGG | 0.654214 | 4_3:+68107941 | Msa0658070:CDS |
| AGTCTACGGTCTCTCCAAAG+AGG | 0.659563 | 4_3:+68108181 | Msa0658070:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | 4_3:+68107881 | Msa0658070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTAAAGAAAAGAGTGAAAA+TGG | - | chr4_3:68108055-68108074 | None:intergenic | 25.0% |
| ! | TTCATACCTTCAAATGAAAT+TGG | + | chr4_3:68108333-68108352 | Msa0658070:CDS | 25.0% |
| ATCTGAATCAGAAACATCAA+TGG | - | chr4_3:68108005-68108024 | None:intergenic | 30.0% | |
| ! | GATGTTTCTGATTCAGATAA+TGG | + | chr4_3:68108007-68108026 | Msa0658070:CDS | 30.0% |
| TTCATATTCATGCAAGTCTA+CGG | + | chr4_3:68108167-68108186 | Msa0658070:CDS | 30.0% | |
| ! | GTTCACATTTTCGAACAATA+AGG | - | chr4_3:68108310-68108329 | None:intergenic | 30.0% |
| TGGACTAAGATACCAAAACT+GGG | + | chr4_3:68107880-68107899 | Msa0658070:CDS | 35.0% | |
| TTTGGAAAACAACGTTTGAG+AGG | - | chr4_3:68107923-68107942 | None:intergenic | 35.0% | |
| ! | CTGTTAGTAAGGAGTTCTTT+TGG | - | chr4_3:68107941-68107960 | None:intergenic | 35.0% |
| AAAGAACTCCTTACTAACAG+TGG | + | chr4_3:68107941-68107960 | Msa0658070:CDS | 35.0% | |
| !! | TGCTATATACTACTTTTGCG+AGG | + | chr4_3:68108076-68108095 | Msa0658070:intron | 35.0% |
| !! | GCTATATACTACTTTTGCGA+GGG | + | chr4_3:68108077-68108096 | Msa0658070:intron | 35.0% |
| GAGGATTATCAATCAGAAGA+TGG | + | chr4_3:68108212-68108231 | Msa0658070:CDS | 35.0% | |
| CCAAGACCAATTTCATTTGA+AGG | - | chr4_3:68108342-68108361 | None:intergenic | 35.0% | |
| ! | CCTTCAAATGAAATTGGTCT+TGG | + | chr4_3:68108339-68108358 | Msa0658070:CDS | 35.0% |
| ! | CATAAAACTGGCTTCTCTTC+TGG | - | chr4_3:68107851-68107870 | None:intergenic | 40.0% |
| CCACATGCATTGCATAAAAC+TGG | - | chr4_3:68107863-68107882 | None:intergenic | 40.0% | |
| !! | CCAGTTTTATGCAATGCATG+TGG | + | chr4_3:68107860-68107879 | Msa0658070:CDS | 40.0% |
| ! | GTGGACTAAGATACCAAAAC+TGG | + | chr4_3:68107879-68107898 | Msa0658070:CDS | 40.0% |
| GGACTAAGATACCAAAACTG+GGG | + | chr4_3:68107881-68107900 | Msa0658070:CDS | 40.0% | |
| ! | CTTCAAGATTTCCCCAGTTT+TGG | - | chr4_3:68107895-68107914 | None:intergenic | 40.0% |
| TACTGTTGCCACTGTTAGTA+AGG | - | chr4_3:68107952-68107971 | None:intergenic | 40.0% | |
| !! | ATATACTACTTTTGCGAGGG+TGG | + | chr4_3:68108080-68108099 | Msa0658070:intron | 40.0% |
| !! | TATACTACTTTTGCGAGGGT+GGG | + | chr4_3:68108081-68108100 | Msa0658070:intron | 40.0% |
| GAACAATAAGGTCTCTTCTG+GGG | - | chr4_3:68108298-68108317 | None:intergenic | 40.0% | |
| CGAACAATAAGGTCTCTTCT+GGG | - | chr4_3:68108299-68108318 | None:intergenic | 40.0% | |
| TCGAACAATAAGGTCTCTTC+TGG | - | chr4_3:68108300-68108319 | None:intergenic | 40.0% | |
| GCAGTTCTTCTTAAACCAGA+CGG | + | chr4_3:68108363-68108382 | Msa0658070:CDS | 40.0% | |
| ! | TTTTTCCAGCAACTCCAGCA+TGG | + | chr4_3:68107816-68107835 | Msa0658070:CDS | 45.0% |
| ATCCTCAAACTACGCCTCTT+TGG | - | chr4_3:68108198-68108217 | None:intergenic | 45.0% | |
| AGTCTACGGTCTCTCCAAAG+AGG | + | chr4_3:68108181-68108200 | Msa0658070:CDS | 50.0% | |
| ! | CTCCAAAGAGGCGTAGTTTG+AGG | + | chr4_3:68108193-68108212 | Msa0658070:CDS | 50.0% |
| ! | TCTTCTGGGGATGACTCATC+TGG | - | chr4_3:68108285-68108304 | None:intergenic | 50.0% |
| CTGGCTTCTCTTCTGGTCCC+TGG | - | chr4_3:68107844-68107863 | None:intergenic | 60.0% | |
| TGGCGCCATGCTGGAGTTGC+TGG | - | chr4_3:68107824-68107843 | None:intergenic | 65.0% | |
| AGCAACTCCAGCATGGCGCC+AGG | + | chr4_3:68107823-68107842 | Msa0658070:CDS | 65.0% | |
| GCAACTCCAGCATGGCGCCA+GGG | + | chr4_3:68107824-68107843 | Msa0658070:CDS | 65.0% | |
| TCTGGTCCCTGGCGCCATGC+TGG | - | chr4_3:68107833-68107852 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 68107812 | 68108398 | 68107812 | ID=Msa0658070;Name=Msa0658070 |
| chr4_3 | mRNA | 68107812 | 68108398 | 68107812 | ID=Msa0658070-mRNA-1;Parent=Msa0658070;Name=Msa0658070-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|152 |
| chr4_3 | exon | 68107812 | 68108028 | 68107812 | ID=Msa0658070-mRNA-1:exon:19668;Parent=Msa0658070-mRNA-1 |
| chr4_3 | exon | 68108157 | 68108398 | 68108157 | ID=Msa0658070-mRNA-1:exon:19669;Parent=Msa0658070-mRNA-1 |
| chr4_3 | CDS | 68107812 | 68108028 | 68107812 | ID=Msa0658070-mRNA-1:cds;Parent=Msa0658070-mRNA-1 |
| chr4_3 | CDS | 68108157 | 68108398 | 68108157 | ID=Msa0658070-mRNA-1:cds;Parent=Msa0658070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
