AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0662960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662960 MtrunA17_Chr8g0352871 83.750 160 18 1 1 152 1 160 3.79e-94 270
Msa0662960 MtrunA17_Chr3g0082861 75.484 155 29 1 7 152 2 156 1.43e-82 240
Msa0662960 MtrunA17_Chr7g0256551 70.064 157 38 1 1 148 1 157 4.42e-79 234
Msa0662960 MtrunA17_Chr4g0021731 96.512 86 3 0 1 86 3 88 1.77e-56 171
Msa0662960 MtrunA17_Chr3g0124861 55.346 159 52 3 9 149 22 179 6.58e-56 175
Msa0662960 MtrunA17_Chr4g0059571 58.741 143 59 0 4 146 21 163 7.65e-54 169
Msa0662960 MtrunA17_Chr3g0128691 61.069 131 47 2 10 137 19 148 1.11e-51 161
Msa0662960 MtrunA17_Chr5g0414931 56.164 146 58 2 8 148 15 159 2.20e-51 163
Msa0662960 MtrunA17_Chr3g0118211 52.349 149 71 0 4 152 18 166 2.53e-51 162
Msa0662960 MtrunA17_Chr1g0170741 60.584 137 51 2 8 141 7 143 3.09e-50 159
Msa0662960 MtrunA17_Chr4g0030271 30.303 132 83 2 7 138 87 209 2.31e-15 72.0
Msa0662960 MtrunA17_Chr8g0345901 29.771 131 83 1 11 141 38 159 2.42e-15 72.0
Msa0662960 MtrunA17_Chr8g0376881 31.469 143 86 3 4 146 24 154 4.69e-15 71.2
Msa0662960 MtrunA17_Chr1g0181811 28.906 128 82 2 11 138 87 205 1.88e-13 66.6
Msa0662960 MtrunA17_Chr7g0275891 32.143 140 82 4 11 150 11 137 2.55e-13 65.9
Msa0662960 MtrunA17_Chr3g0123131 29.412 136 84 2 11 146 19 142 8.16e-13 64.7
Msa0662960 MtrunA17_Chr1g0149841 30.000 140 84 3 11 150 11 136 8.24e-13 64.7
Msa0662960 MtrunA17_Chr1g0161431 30.935 139 84 3 9 147 15 141 1.02e-12 64.7
Msa0662960 MtrunA17_Chr1g0149851 32.308 130 76 3 11 140 11 128 2.06e-12 63.5
Msa0662960 MtrunA17_Chr7g0275401 31.061 132 79 3 9 140 9 128 2.08e-12 63.2
Msa0662960 MtrunA17_Chr7g0276361 26.515 132 88 1 7 138 12 134 2.11e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662960 AT3G57040.2 68.387 155 39 1 1 145 1 155 8.48e-74 221
Msa0662960 AT3G57040.1 68.387 155 39 1 1 145 1 155 8.48e-74 221
Msa0662960 AT2G41310.1 64.516 155 47 1 1 147 1 155 6.15e-71 213
Msa0662960 AT1G59940.1 62.595 131 49 0 8 138 32 162 9.93e-56 174
Msa0662960 AT1G59940.2 62.595 131 49 0 8 138 124 254 3.35e-54 174
Msa0662960 AT3G48100.1 58.993 139 56 1 2 139 18 156 4.05e-54 169
Msa0662960 AT1G10470.3 61.069 131 51 0 8 138 19 149 4.47e-54 171
Msa0662960 AT1G10470.1 61.069 131 51 0 8 138 33 163 6.33e-54 171
Msa0662960 AT5G62920.1 62.308 130 49 0 9 138 25 154 1.53e-52 165
Msa0662960 AT1G19050.1 55.882 136 60 0 8 143 23 158 3.57e-52 165
Msa0662960 AT1G74890.1 57.664 137 58 0 8 144 17 153 5.89e-52 164
Msa0662960 AT3G56380.1 54.348 138 62 1 4 140 15 152 3.42e-51 160
Msa0662960 AT3G56380.2 53.957 139 62 2 4 140 15 153 1.63e-49 156
Msa0662960 AT2G40670.1 53.237 139 56 2 8 140 28 163 3.29e-49 155
Msa0662960 AT2G40670.2 52.857 140 56 3 8 140 28 164 2.22e-47 151
Msa0662960 AT2G07440.1 67.213 61 20 0 87 147 9 69 5.34e-23 88.6
Msa0662960 AT1G10470.2 60.377 53 21 0 86 138 35 87 2.29e-16 72.4
Msa0662960 AT2G25180.1 33.077 130 75 3 11 140 19 136 5.84e-14 68.6
Msa0662960 AT5G07210.2 29.167 144 89 3 8 151 15 145 1.32e-13 67.4
Msa0662960 AT5G60100.7 31.250 128 79 2 11 138 66 184 4.13e-13 65.9
Msa0662960 AT5G60100.6 31.250 128 79 2 11 138 66 184 4.13e-13 65.9
Msa0662960 AT5G60100.4 31.250 128 79 2 11 138 66 184 4.13e-13 65.9
Msa0662960 AT5G60100.2 31.250 128 79 2 11 138 66 184 5.69e-13 65.5
Msa0662960 AT5G02810.1 28.125 128 83 2 11 138 80 198 6.21e-13 65.5
Msa0662960 AT1G67710.1 28.889 135 84 2 9 143 11 133 7.67e-13 65.1
Msa0662960 AT5G60100.5 31.250 128 79 2 11 138 66 184 1.13e-12 64.7
Msa0662960 AT5G60100.3 31.250 128 79 2 11 138 66 184 1.13e-12 64.7
Msa0662960 AT5G60100.1 31.250 128 79 2 11 138 66 184 1.13e-12 64.7
Msa0662960 AT3G16857.1 29.630 135 83 3 9 143 37 159 7.35e-12 62.4
Msa0662960 AT3G16857.2 29.630 135 83 3 9 143 37 159 7.83e-12 62.4
Msa0662960 AT2G46790.3 25.850 147 99 3 6 152 34 170 1.17e-11 61.6
Msa0662960 AT2G46790.1 25.850 147 99 3 6 152 34 170 1.40e-11 61.6
Msa0662960 AT1G49190.1 29.545 132 81 3 10 141 35 154 2.04e-11 60.8
Msa0662960 AT5G24470.1 28.571 133 84 2 9 140 50 172 3.87e-11 60.5
Msa0662960 AT1G49190.2 29.545 132 81 3 10 141 35 154 7.31e-11 59.7

Find 24 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATTGTTGAACTGGTTTC+AGG 0.173333 4_4:-987305 None:intergenic
CGGTATGTCTTTAAAAGATT+TGG 0.218132 4_4:-987067 None:intergenic
AGAGACTCAGTTCCATGTTT+TGG 0.251951 4_4:+986165 Msa0662960:CDS
AGTAAGGCTTTAAAATTTCT+TGG 0.266953 4_4:+986471 Msa0662960:CDS
TCTCACCTGCTAATCCTTGA+TGG 0.304860 4_4:-987117 None:intergenic
GGCTTTAAAATTTCTTGGTT+TGG 0.308229 4_4:+986476 Msa0662960:CDS
TTTACATCTGATTGTTGAAC+TGG 0.343425 4_4:-987312 None:intergenic
TCTTGGTTTGGTTGAGGATG+TGG 0.414547 4_4:+986488 Msa0662960:CDS
TTTGATGAAATCAAGAGTTA+AGG 0.416978 4_4:+987350 Msa0662960:CDS
TACAATGGACTCTGCTAGTA+AGG 0.427203 4_4:+986455 Msa0662960:CDS
TATTGTATGCCAGGGATGAC+TGG 0.449232 4_4:+986654 Msa0662960:CDS
AAAATTTCTTGGTTTGGTTG+AGG 0.449963 4_4:+986482 Msa0662960:CDS
ACTCTTGATTTCATCAAATG+AGG 0.455768 4_4:-987345 None:intergenic
ATAACAGATTATTGTATGCC+AGG 0.463952 4_4:+986645 Msa0662960:CDS
ATGTTCCATCAAGGATTAGC+AGG 0.504757 4_4:+987112 Msa0662960:CDS
TCTGAAGACATAATCACAAC+CGG 0.515090 4_4:-987087 None:intergenic
GATGTGGCTTCAGAAGTTCA+TGG 0.523538 4_4:+986504 Msa0662960:CDS
ATGTGGCTTCAGAAGTTCAT+GGG 0.534908 4_4:+986505 Msa0662960:CDS
CTTCAGAGAATGTTCCATCA+AGG 0.539392 4_4:+987103 Msa0662960:CDS
AGATCATAGCCAGTCATCCC+TGG 0.546401 4_4:-986663 None:intergenic
TAACAGATTATTGTATGCCA+GGG 0.564090 4_4:+986646 Msa0662960:CDS
TATCATCAACAGCCAAAACA+TGG 0.580138 4_4:-986177 None:intergenic
TGAAATGATAGTTACTACAA+TGG 0.624009 4_4:+986440 Msa0662960:intron
TGTGGCTTCAGAAGTTCATG+GGG 0.695893 4_4:+986506 Msa0662960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATAATTATTATTTATTTTG+TGG + chr4_4:986409-986428 Msa0662960:intron 10.0%
!!! AATTTTTCTTAATATTATGA+TGG + chr4_4:986543-986562 Msa0662960:intron 10.0%
!!! ATTATTTTGTTTTTCTATTT+AGG + chr4_4:986846-986865 Msa0662960:intron 10.0%
!!! TTTGTTTTCTTTATAATTTT+GGG - chr4_4:986987-987006 None:intergenic 10.0%
!!! GATAATTATTATTTATTTTG+TGG + chr4_4:986409-986428 Msa0662960:intron 10.0%
!!! AATTTTTCTTAATATTATGA+TGG + chr4_4:986543-986562 Msa0662960:intron 10.0%
!!! ATTATTTTGTTTTTCTATTT+AGG + chr4_4:986846-986865 Msa0662960:intron 10.0%
!!! TTTGTTTTCTTTATAATTTT+GGG - chr4_4:986987-987006 None:intergenic 10.0%
!!! CTTTGTTTTCTTTATAATTT+TGG - chr4_4:986988-987007 None:intergenic 15.0%
!!! CTTTGTTTTCTTTATAATTT+TGG - chr4_4:986988-987007 None:intergenic 15.0%
!!! TCAATATACTATTTTGTTGA+AGG + chr4_4:986355-986374 Msa0662960:intron 20.0%
!! TTTCTTAAATAGAAGTGATT+TGG + chr4_4:986894-986913 Msa0662960:intron 20.0%
!!! TAACATCAAGTTAACAATTT+TGG - chr4_4:987017-987036 None:intergenic 20.0%
!! TAAACAATTCTTTGATTCTA+AGG + chr4_4:987041-987060 Msa0662960:intron 20.0%
!!! TCAATATACTATTTTGTTGA+AGG + chr4_4:986355-986374 Msa0662960:intron 20.0%
!! TTTCTTAAATAGAAGTGATT+TGG + chr4_4:986894-986913 Msa0662960:intron 20.0%
!!! TAACATCAAGTTAACAATTT+TGG - chr4_4:987017-987036 None:intergenic 20.0%
!! TAAACAATTCTTTGATTCTA+AGG + chr4_4:987041-987060 Msa0662960:intron 20.0%
! ATTGTAAAGATGTAATGTGA+TGG + chr4_4:986301-986320 Msa0662960:intron 25.0%
! TTGTAAAGATGTAATGTGAT+GGG + chr4_4:986302-986321 Msa0662960:intron 25.0%
! TGAAATGATAGTTACTACAA+TGG + chr4_4:986440-986459 Msa0662960:intron 25.0%
!!! AGTAAGGCTTTAAAATTTCT+TGG + chr4_4:986471-986490 Msa0662960:CDS 25.0%
! TTGATTCATATTATTCATGC+AGG + chr4_4:986602-986621 Msa0662960:intron 25.0%
! TGAAAATGAGCACAAAATTA+TGG - chr4_4:986796-986815 None:intergenic 25.0%
!!! TGATTAGTGATTACTGATTT+GGG + chr4_4:986956-986975 Msa0662960:intron 25.0%
!!! CAAATCTTTTAAAGACATAC+CGG + chr4_4:987068-987087 Msa0662960:CDS 25.0%
! TTTGATGAAATCAAGAGTTA+AGG + chr4_4:987350-987369 Msa0662960:CDS 25.0%
! ATTGTAAAGATGTAATGTGA+TGG + chr4_4:986301-986320 Msa0662960:intron 25.0%
! TTGTAAAGATGTAATGTGAT+GGG + chr4_4:986302-986321 Msa0662960:intron 25.0%
! TGAAATGATAGTTACTACAA+TGG + chr4_4:986440-986459 Msa0662960:intron 25.0%
!!! AGTAAGGCTTTAAAATTTCT+TGG + chr4_4:986471-986490 Msa0662960:CDS 25.0%
! TTGATTCATATTATTCATGC+AGG + chr4_4:986602-986621 Msa0662960:intron 25.0%
! TGAAAATGAGCACAAAATTA+TGG - chr4_4:986796-986815 None:intergenic 25.0%
!!! TGATTAGTGATTACTGATTT+GGG + chr4_4:986956-986975 Msa0662960:intron 25.0%
!!! CAAATCTTTTAAAGACATAC+CGG + chr4_4:987068-987087 Msa0662960:CDS 25.0%
! TTTGATGAAATCAAGAGTTA+AGG + chr4_4:987350-987369 Msa0662960:CDS 25.0%
! CATGAAAAGAAGAAGTTTTG+AGG - chr4_4:986234-986253 None:intergenic 30.0%
! CTCAAAACTTCTTCTTTTCA+TGG + chr4_4:986232-986251 Msa0662960:CDS 30.0%
TGTAAAGATGTAATGTGATG+GGG + chr4_4:986303-986322 Msa0662960:intron 30.0%
! ACATATGAGCATAGTTTGTA+TGG + chr4_4:986332-986351 Msa0662960:intron 30.0%
!! GGCTTTAAAATTTCTTGGTT+TGG + chr4_4:986476-986495 Msa0662960:CDS 30.0%
!! AAAATTTCTTGGTTTGGTTG+AGG + chr4_4:986482-986501 Msa0662960:CDS 30.0%
!!! GACTATTTTGATGTATACGT+TGG + chr4_4:986570-986589 Msa0662960:intron 30.0%
ATAACAGATTATTGTATGCC+AGG + chr4_4:986645-986664 Msa0662960:CDS 30.0%
TAACAGATTATTGTATGCCA+GGG + chr4_4:986646-986665 Msa0662960:CDS 30.0%
! CTGATTAGTGATTACTGATT+TGG + chr4_4:986955-986974 Msa0662960:intron 30.0%
! CGGTATGTCTTTAAAAGATT+TGG - chr4_4:987070-987089 None:intergenic 30.0%
ATGTGAAGTCTCACATATTA+TGG + chr4_4:987228-987247 Msa0662960:intron 30.0%
!!! TTGTATCAGATGTTTTGAAG+AGG + chr4_4:987266-987285 Msa0662960:intron 30.0%
!!! TGTATCAGATGTTTTGAAGA+GGG + chr4_4:987267-987286 Msa0662960:intron 30.0%
TTTACATCTGATTGTTGAAC+TGG - chr4_4:987315-987334 None:intergenic 30.0%
ACTCTTGATTTCATCAAATG+AGG - chr4_4:987348-987367 None:intergenic 30.0%
! CATGAAAAGAAGAAGTTTTG+AGG - chr4_4:986234-986253 None:intergenic 30.0%
! CTCAAAACTTCTTCTTTTCA+TGG + chr4_4:986232-986251 Msa0662960:CDS 30.0%
TGTAAAGATGTAATGTGATG+GGG + chr4_4:986303-986322 Msa0662960:intron 30.0%
! ACATATGAGCATAGTTTGTA+TGG + chr4_4:986332-986351 Msa0662960:intron 30.0%
!! GGCTTTAAAATTTCTTGGTT+TGG + chr4_4:986476-986495 Msa0662960:CDS 30.0%
!! AAAATTTCTTGGTTTGGTTG+AGG + chr4_4:986482-986501 Msa0662960:CDS 30.0%
!!! GACTATTTTGATGTATACGT+TGG + chr4_4:986570-986589 Msa0662960:intron 30.0%
ATAACAGATTATTGTATGCC+AGG + chr4_4:986645-986664 Msa0662960:CDS 30.0%
TAACAGATTATTGTATGCCA+GGG + chr4_4:986646-986665 Msa0662960:CDS 30.0%
! CTGATTAGTGATTACTGATT+TGG + chr4_4:986955-986974 Msa0662960:intron 30.0%
! CGGTATGTCTTTAAAAGATT+TGG - chr4_4:987070-987089 None:intergenic 30.0%
ATGTGAAGTCTCACATATTA+TGG + chr4_4:987228-987247 Msa0662960:intron 30.0%
!!! TTGTATCAGATGTTTTGAAG+AGG + chr4_4:987266-987285 Msa0662960:intron 30.0%
!!! TGTATCAGATGTTTTGAAGA+GGG + chr4_4:987267-987286 Msa0662960:intron 30.0%
TTTACATCTGATTGTTGAAC+TGG - chr4_4:987315-987334 None:intergenic 30.0%
ACTCTTGATTTCATCAAATG+AGG - chr4_4:987348-987367 None:intergenic 30.0%
TATCATCAACAGCCAAAACA+TGG - chr4_4:986180-986199 None:intergenic 35.0%
TGATCTGCTCAGAAAAATCA+AGG + chr4_4:986680-986699 Msa0662960:CDS 35.0%
TCTGAAGACATAATCACAAC+CGG - chr4_4:987090-987109 None:intergenic 35.0%
TATCATCAACAGCCAAAACA+TGG - chr4_4:986180-986199 None:intergenic 35.0%
TGATCTGCTCAGAAAAATCA+AGG + chr4_4:986680-986699 Msa0662960:CDS 35.0%
TCTGAAGACATAATCACAAC+CGG - chr4_4:987090-987109 None:intergenic 35.0%
! AGAGACTCAGTTCCATGTTT+TGG + chr4_4:986165-986184 Msa0662960:CDS 40.0%
! TACAATGGACTCTGCTAGTA+AGG + chr4_4:986455-986474 Msa0662960:CDS 40.0%
ATGTGGCTTCAGAAGTTCAT+GGG + chr4_4:986505-986524 Msa0662960:CDS 40.0%
CTTCAGAGAATGTTCCATCA+AGG + chr4_4:987103-987122 Msa0662960:CDS 40.0%
! ATGTTCCATCAAGGATTAGC+AGG + chr4_4:987112-987131 Msa0662960:CDS 40.0%
! CTGATTGTTGAACTGGTTTC+AGG - chr4_4:987308-987327 None:intergenic 40.0%
! AGAGACTCAGTTCCATGTTT+TGG + chr4_4:986165-986184 Msa0662960:CDS 40.0%
! TACAATGGACTCTGCTAGTA+AGG + chr4_4:986455-986474 Msa0662960:CDS 40.0%
ATGTGGCTTCAGAAGTTCAT+GGG + chr4_4:986505-986524 Msa0662960:CDS 40.0%
CTTCAGAGAATGTTCCATCA+AGG + chr4_4:987103-987122 Msa0662960:CDS 40.0%
! ATGTTCCATCAAGGATTAGC+AGG + chr4_4:987112-987131 Msa0662960:CDS 40.0%
! CTGATTGTTGAACTGGTTTC+AGG - chr4_4:987308-987327 None:intergenic 40.0%
!! TCTTGGTTTGGTTGAGGATG+TGG + chr4_4:986488-986507 Msa0662960:CDS 45.0%
GATGTGGCTTCAGAAGTTCA+TGG + chr4_4:986504-986523 Msa0662960:CDS 45.0%
TGTGGCTTCAGAAGTTCATG+GGG + chr4_4:986506-986525 Msa0662960:CDS 45.0%
TATTGTATGCCAGGGATGAC+TGG + chr4_4:986654-986673 Msa0662960:CDS 45.0%
TCTCACCTGCTAATCCTTGA+TGG - chr4_4:987120-987139 None:intergenic 45.0%
!! TCTTGGTTTGGTTGAGGATG+TGG + chr4_4:986488-986507 Msa0662960:CDS 45.0%
GATGTGGCTTCAGAAGTTCA+TGG + chr4_4:986504-986523 Msa0662960:CDS 45.0%
TGTGGCTTCAGAAGTTCATG+GGG + chr4_4:986506-986525 Msa0662960:CDS 45.0%
TATTGTATGCCAGGGATGAC+TGG + chr4_4:986654-986673 Msa0662960:CDS 45.0%
TCTCACCTGCTAATCCTTGA+TGG - chr4_4:987120-987139 None:intergenic 45.0%
AGATCATAGCCAGTCATCCC+TGG - chr4_4:986666-986685 None:intergenic 50.0%
AGATCATAGCCAGTCATCCC+TGG - chr4_4:986666-986685 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 986151 987404 986151 ID=Msa0662960;Name=Msa0662960
chr4_4 mRNA 986151 987404 986151 ID=Msa0662960-mRNA-1;Parent=Msa0662960;Name=Msa0662960-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|1|1|5|0|152
chr4_4 exon 986151 986253 986151 ID=Msa0662960-mRNA-1:exon:378;Parent=Msa0662960-mRNA-1
chr4_4 exon 986451 986527 986451 ID=Msa0662960-mRNA-1:exon:379;Parent=Msa0662960-mRNA-1
chr4_4 exon 986624 986701 986624 ID=Msa0662960-mRNA-1:exon:380;Parent=Msa0662960-mRNA-1
chr4_4 exon 987063 987133 987063 ID=Msa0662960-mRNA-1:exon:381;Parent=Msa0662960-mRNA-1
chr4_4 exon 987275 987404 987275 ID=Msa0662960-mRNA-1:exon:382;Parent=Msa0662960-mRNA-1
chr4_4 CDS 986151 986253 986151 ID=Msa0662960-mRNA-1:cds;Parent=Msa0662960-mRNA-1
chr4_4 CDS 986451 986527 986451 ID=Msa0662960-mRNA-1:cds;Parent=Msa0662960-mRNA-1
chr4_4 CDS 986624 986701 986624 ID=Msa0662960-mRNA-1:cds;Parent=Msa0662960-mRNA-1
chr4_4 CDS 987063 987133 987063 ID=Msa0662960-mRNA-1:cds;Parent=Msa0662960-mRNA-1
chr4_4 CDS 987275 987404 987275 ID=Msa0662960-mRNA-1:cds;Parent=Msa0662960-mRNA-1
Gene Sequence

>Msa0662960

ATGGGTATGGCTGCAGAGACTCAGTTCCATGTTTTGGCTGTTGATGATAGTATTATTGATAGAATGTTGATTGAGAGACTCCTCAAAACTTCTTCTTTTCATGTTACTACAATGGACTCTGCTAGTAAGGCTTTAAAATTTCTTGGTTTGGTTGAGGATGTGGCTTCAGAAGTTCATGGGGATGTAGATGTAAATCTGATCATAACAGATTATTGTATGCCAGGGATGACTGGCTATGATCTGCTCAGAAAAATCAAGGAATCCAAATCTTTTAAAGACATACCGGTTGTGATTATGTCTTCAGAGAATGTTCCATCAAGGATTAGCAGATGTTTTGAAGAGGGAGCTGAAGAATTCTTCCTGAAACCAGTTCAACAATCAGATGTAAACAAGCTGAAGCCTCATTTGATGAAATCAAGAGTTAAGGAAGAGCAAGAACAAACAACCCATCAACAATAA

Protein sequence

>Msa0662960

MGMAAETQFHVLAVDDSIIDRMLIERLLKTSSFHVTTMDSASKALKFLGLVEDVASEVHGDVDVNLIITDYCMPGMTGYDLLRKIKESKSFKDIPVVIMSSENVPSRISRCFEEGAEEFFLKPVQQSDVNKLKPHLMKSRVKEEQEQTTHQQ*