AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0666810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0666810 MtrunA17_Chr4g0005201 94.444 144 7 1 1 144 652 794 2.79e-80 251
Msa0666810 MtrunA17_Chr5g0437741 72.222 144 39 1 1 144 644 786 7.29e-64 207
Msa0666810 MtrunA17_Chr4g0005221 70.000 100 29 1 46 144 449 548 2.26e-39 139
Msa0666810 MtrunA17_Chr6g0478621 49.550 111 51 3 17 124 540 648 6.22e-27 104
Msa0666810 MtrunA17_Chr4g0062911 48.649 111 52 3 17 124 509 617 5.96e-26 102
Msa0666810 MtrunA17_Chr5g0432851 46.281 121 59 4 8 124 509 627 9.03e-26 101
Msa0666810 MtrunA17_Chr7g0245171 46.847 111 54 3 17 124 498 606 2.00e-25 100
Msa0666810 MtrunA17_Chr8g0393211 45.946 111 55 3 17 124 537 645 4.13e-25 99.8
Msa0666810 MtrunA17_Chr3g0128551 46.364 110 56 2 17 124 506 614 5.29e-25 99.4
Msa0666810 MtrunA17_Chr3g0108301 46.847 111 54 3 17 124 518 626 6.11e-25 99.4
Msa0666810 MtrunA17_Chr8g0387791 42.520 127 68 3 1 124 471 595 6.63e-25 99.0
Msa0666810 MtrunA17_Chr5g0396941 44.444 117 60 3 17 130 464 578 9.77e-25 98.6
Msa0666810 MtrunA17_Chr1g0209521 46.457 127 58 4 11 129 518 642 1.02e-24 98.6
Msa0666810 MtrunA17_Chr3g0126281 44.348 115 59 3 13 124 215 327 1.42e-24 96.7
Msa0666810 MtrunA17_Chr5g0434551 43.411 129 65 5 17 139 502 628 1.92e-24 97.8
Msa0666810 MtrunA17_Chr5g0428541 45.217 115 57 3 15 124 474 587 6.46e-23 93.6
Msa0666810 MtrunA17_Chr7g0237801 41.860 129 72 2 1 126 533 661 2.11e-22 92.0
Msa0666810 MtrunA17_Chr5g0423121 42.735 117 61 4 15 127 496 610 6.51e-22 90.5
Msa0666810 MtrunA17_Chr1g0159161 45.614 114 55 4 17 127 450 559 3.80e-21 88.2
Msa0666810 MtrunA17_Chr3g0126271 43.363 113 59 3 17 126 485 595 4.76e-21 88.2
Msa0666810 MtrunA17_Chr6g0478181 41.739 115 51 5 20 124 437 545 5.55e-19 82.0
Msa0666810 MtrunA17_Chr2g0279071 36.842 133 74 4 10 136 289 417 1.11e-18 81.3
Msa0666810 MtrunA17_Chr7g0265601 38.525 122 59 3 17 124 540 659 2.24e-18 80.5
Msa0666810 MtrunA17_Chr5g0445251 40.310 129 67 4 15 143 88 206 4.59e-18 77.4
Msa0666810 MtrunA17_Chr8g0376301 46.591 88 45 2 30 117 287 372 7.17e-17 75.9
Msa0666810 MtrunA17_Chr5g0437731 70.909 55 15 1 1 55 76 129 7.92e-17 72.0
Msa0666810 MtrunA17_Chr2g0312651 40.833 120 68 3 4 121 964 1082 1.42e-16 75.5
Msa0666810 MtrunA17_Chr1g0147701 38.261 115 58 5 18 124 505 614 4.00e-16 73.9
Msa0666810 MtrunA17_Chr8g0356041 35.338 133 73 4 4 125 221 351 5.93e-16 73.2
Msa0666810 MtrunA17_Chr2g0288391 46.237 93 46 3 32 124 517 605 6.74e-16 73.2
Msa0666810 MtrunA17_Chr3g0129391 38.281 128 73 4 1 124 1025 1150 7.69e-16 73.2
Msa0666810 MtrunA17_Chr7g0257621 38.710 124 74 2 3 124 762 885 1.52e-15 72.4
Msa0666810 MtrunA17_Chr1g0169841 42.373 118 61 5 11 123 973 1088 1.90e-15 72.0
Msa0666810 MtrunA17_Chr7g0237821 41.228 114 61 3 13 124 964 1073 3.81e-15 71.2
Msa0666810 MtrunA17_Chr4g0054521 37.607 117 66 3 11 124 851 963 4.62e-15 70.9
Msa0666810 MtrunA17_Chr3g0117921 43.182 88 46 3 30 117 541 624 6.57e-15 70.5
Msa0666810 MtrunA17_Chr7g0252301 35.433 127 71 4 3 124 1009 1129 6.87e-15 70.5
Msa0666810 MtrunA17_Chr2g0280191 37.607 117 66 4 13 126 855 967 7.19e-15 70.5
Msa0666810 MtrunA17_Chr2g0313911 37.719 114 65 3 13 123 235 345 8.63e-15 69.7
Msa0666810 MtrunA17_Chr2g0324931 37.069 116 68 3 13 127 858 969 1.03e-14 70.1
Msa0666810 MtrunA17_Chr5g0419721 40.496 121 65 3 4 124 807 920 1.15e-14 69.7
Msa0666810 MtrunA17_Chr5g0398441 36.283 113 65 3 15 124 777 885 1.15e-14 69.7
Msa0666810 MtrunA17_Chr3g0096351 38.393 112 63 3 14 122 345 453 1.16e-14 69.7
Msa0666810 MtrunA17_Chr4g0046511 34.513 113 69 3 13 124 861 969 1.17e-14 69.7
Msa0666810 MtrunA17_Chr4g0060011 38.261 115 62 4 13 123 477 586 1.61e-14 69.3
Msa0666810 MtrunA17_Chr3g0126181 41.026 117 62 4 13 124 930 1044 1.80e-14 69.3
Msa0666810 MtrunA17_Chr3g0140861 36.719 128 72 4 4 126 816 939 2.70e-14 68.9
Msa0666810 MtrunA17_Chr5g0400681 36.719 128 75 4 11 136 854 977 4.26e-14 68.2
Msa0666810 MtrunA17_Chr7g0261331 33.058 121 78 2 1 121 1082 1199 5.10e-14 68.2
Msa0666810 MtrunA17_Chr1g0165141 32.407 108 72 1 14 121 1020 1126 5.48e-14 67.8
Msa0666810 MtrunA17_Chr3g0079661 36.154 130 73 3 13 140 1079 1200 5.89e-14 67.8
Msa0666810 MtrunA17_Chr2g0292521 37.121 132 73 5 1 124 937 1066 6.36e-14 67.8
Msa0666810 MtrunA17_Chr7g0239371 36.364 121 71 3 5 122 493 610 7.00e-14 67.4
Msa0666810 MtrunA17_Chr8g0355401 30.172 116 80 1 6 121 42 156 7.48e-14 64.7
Msa0666810 MtrunA17_Chr1g0200471 38.739 111 63 3 11 121 812 917 9.24e-14 67.4
Msa0666810 MtrunA17_Chr2g0277081 36.207 116 67 3 11 123 849 960 9.41e-14 67.4
Msa0666810 MtrunA17_Chr3g0102901 36.441 118 71 3 13 127 941 1057 1.12e-13 67.0
Msa0666810 MtrunA17_Chr7g0248904 31.008 129 83 3 11 136 522 647 1.39e-13 66.6
Msa0666810 MtrunA17_Chr7g0271451 39.286 112 62 3 14 122 359 467 1.55e-13 66.6
Msa0666810 MtrunA17_Chr1g0165211 34.167 120 73 3 6 121 1054 1171 1.70e-13 66.6
Msa0666810 MtrunA17_Chr2g0334251 35.593 118 69 3 18 130 542 657 1.72e-13 66.6
Msa0666810 MtrunA17_Chr1g0201241 35.897 117 69 2 13 124 886 1001 2.45e-13 66.2
Msa0666810 MtrunA17_Chr1g0165181 32.407 108 72 1 14 121 179 285 3.20e-13 65.1
Msa0666810 MtrunA17_Chr6g0454621 33.588 131 79 3 1 126 982 1109 3.42e-13 65.5
Msa0666810 MtrunA17_Chr6g0454661 32.824 131 80 3 1 126 981 1108 4.11e-13 65.5
Msa0666810 MtrunA17_Chr6g0488771 35.398 113 65 3 18 124 297 407 4.39e-13 65.1
Msa0666810 MtrunA17_Chr2g0282831 33.913 115 71 3 13 126 833 943 5.08e-13 65.1
Msa0666810 MtrunA17_Chr1g0165561 34.921 126 76 3 14 136 62 184 5.61e-13 63.2
Msa0666810 MtrunA17_Chr1g0183231 32.836 134 74 4 1 126 856 981 5.88e-13 65.1
Msa0666810 MtrunA17_Chr1g0165191 31.481 108 73 1 14 121 1043 1149 6.14e-13 65.1
Msa0666810 MtrunA17_Chr1g0190411 32.812 128 83 2 1 126 862 988 6.18e-13 65.1
Msa0666810 MtrunA17_Chr7g0248491 35.714 112 66 4 14 122 325 433 7.24e-13 64.7
Msa0666810 MtrunA17_Chr7g0250471 35.652 115 72 2 7 121 927 1039 8.13e-13 64.7
Msa0666810 MtrunA17_Chr8g0345561 35.088 114 68 3 14 124 973 1083 8.31e-13 64.7
Msa0666810 MtrunA17_Chr8g0379571 33.913 115 68 3 16 124 543 655 1.13e-12 63.9
Msa0666810 MtrunA17_Chr1g0209211 34.921 126 75 4 1 124 584 704 1.31e-12 63.9
Msa0666810 MtrunA17_Chr5g0442151 33.613 119 70 4 11 124 845 959 1.36e-12 63.9
Msa0666810 MtrunA17_Chr1g0200461 38.938 113 60 4 9 121 822 925 1.45e-12 63.9
Msa0666810 MtrunA17_Chr7g0239381 35.537 121 72 3 5 122 497 614 1.59e-12 63.5
Msa0666810 MtrunA17_Chr2g0317071 32.800 125 75 4 3 124 950 1068 2.44e-12 63.2
Msa0666810 MtrunA17_Chr4g0058761 40.870 115 64 3 13 124 937 1050 2.62e-12 63.2
Msa0666810 MtrunA17_Chr3g0140871 32.639 144 87 5 6 144 847 985 2.72e-12 63.2
Msa0666810 MtrunA17_Chr1g0189871 36.885 122 72 3 4 121 956 1076 3.00e-12 62.8
Msa0666810 MtrunA17_Chr1g0165261 33.613 119 75 3 6 121 476 593 3.13e-12 62.8
Msa0666810 MtrunA17_Chr7g0239211 33.600 125 79 2 5 127 497 619 3.68e-12 62.8
Msa0666810 MtrunA17_Chr5g0447001 37.168 113 67 3 15 124 816 927 4.02e-12 62.4
Msa0666810 MtrunA17_Chr3g0112111 33.858 127 74 4 6 127 504 625 4.06e-12 62.4
Msa0666810 MtrunA17_Chr7g0239221 34.127 126 75 3 5 126 499 620 5.96e-12 62.0
Msa0666810 MtrunA17_Chr2g0301061 30.508 118 77 2 10 123 78 194 6.05e-12 60.5
Msa0666810 MtrunA17_Chr1g0172951 33.600 125 79 2 5 127 497 619 6.60e-12 62.0
Msa0666810 MtrunA17_Chr1g0165231 33.613 119 75 3 6 121 1037 1154 6.64e-12 62.0
Msa0666810 MtrunA17_Chr1g0165301 33.613 119 75 3 6 121 1037 1154 6.64e-12 62.0
Msa0666810 MtrunA17_Chr7g0239361 39.474 114 61 3 14 122 521 631 7.34e-12 61.6
Msa0666810 MtrunA17_Chr5g0446651 36.937 111 67 2 12 121 521 629 8.14e-12 61.6
Msa0666810 MtrunA17_Chr2g0296591 35.398 113 68 3 13 121 1118 1229 8.57e-12 61.6
Msa0666810 MtrunA17_Chr7g0262141 32.593 135 74 5 1 126 865 991 1.12e-11 61.2
Msa0666810 MtrunA17_Chr2g0324471 31.579 114 76 1 8 121 527 638 1.25e-11 61.2
Msa0666810 MtrunA17_Chr1g0179521 39.167 120 59 5 9 124 519 628 1.29e-11 61.2
Msa0666810 MtrunA17_Chr5g0440581 33.333 135 74 6 1 124 863 992 1.38e-11 60.8
Msa0666810 MtrunA17_Chr3g0112391 35.833 120 68 3 14 127 509 625 1.90e-11 60.5
Msa0666810 MtrunA17_Chr5g0409041 34.513 113 67 4 13 122 466 574 1.92e-11 60.5
Msa0666810 MtrunA17_Chr7g0250381 35.780 109 68 2 13 121 935 1041 2.62e-11 60.1
Msa0666810 MtrunA17_Chr2g0302631 35.714 112 66 4 14 122 348 456 2.71e-11 60.1
Msa0666810 MtrunA17_Chr1g0165521 34.921 126 76 3 14 136 625 747 2.96e-11 60.1
Msa0666810 MtrunA17_Chr1g0165901 31.452 124 69 3 7 121 1123 1239 3.50e-11 60.1
Msa0666810 MtrunA17_Chr1g0202201 31.200 125 82 2 3 127 498 618 3.65e-11 59.7
Msa0666810 MtrunA17_Chr3g0102451 34.167 120 75 3 12 129 488 605 4.01e-11 59.7
Msa0666810 MtrunA17_Chr3g0078761 36.937 111 67 2 12 121 508 616 4.47e-11 59.7
Msa0666810 MtrunA17_Chr2g0324481 31.967 122 61 4 14 124 511 621 5.35e-11 59.3
Msa0666810 MtrunA17_Chr7g0232131 35.652 115 70 2 7 121 1070 1180 6.53e-11 58.9
Msa0666810 MtrunA17_Chr2g0296601 34.513 113 69 3 13 121 1118 1229 6.81e-11 58.9
Msa0666810 MtrunA17_Chr8g0375511 34.677 124 70 3 13 126 1006 1128 6.96e-11 58.9
Msa0666810 MtrunA17_Chr2g0324171 30.357 112 76 1 14 125 509 618 7.27e-11 58.9
Msa0666810 MtrunA17_Chr7g0231541 35.652 115 70 2 7 121 603 713 7.97e-11 58.9
Msa0666810 MtrunA17_Chr4g0037561 35.345 116 68 4 12 123 527 639 8.06e-11 58.9
Msa0666810 MtrunA17_Chr7g0248881 30.702 114 75 2 13 124 517 628 8.55e-11 58.5
Msa0666810 MtrunA17_Chr1g0165891 30.400 125 71 3 6 121 554 671 8.62e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0666810 AT3G51740.1 76.190 126 29 1 1 126 686 810 7.42e-60 198
Msa0666810 AT3G08680.1 46.957 115 57 2 15 126 503 616 3.12e-28 108
Msa0666810 AT3G08680.2 46.957 115 57 2 15 126 503 616 3.12e-28 108
Msa0666810 AT2G26730.1 45.038 131 63 4 17 144 511 635 3.82e-28 108
Msa0666810 AT5G58300.3 44.444 117 62 2 10 124 514 629 3.33e-25 100
Msa0666810 AT5G58300.1 44.444 117 62 2 10 124 514 629 3.33e-25 100
Msa0666810 AT5G58300.2 44.444 117 62 2 10 124 514 629 3.33e-25 100
Msa0666810 AT5G05160.1 40.678 118 64 2 12 124 500 616 1.03e-23 96.3
Msa0666810 AT3G24660.1 42.308 130 71 2 1 126 525 654 2.55e-23 95.1
Msa0666810 AT1G68400.1 42.647 136 71 3 13 143 522 655 2.63e-23 95.1
Msa0666810 AT1G64210.1 41.322 121 63 3 17 130 468 587 5.87e-23 94.0
Msa0666810 AT5G41680.2 43.103 116 61 3 12 124 214 327 6.82e-23 92.0
Msa0666810 AT5G41680.1 43.103 116 61 3 12 124 240 353 8.20e-23 92.4
Msa0666810 AT1G48480.1 38.525 122 71 2 17 135 533 653 6.03e-22 90.9
Msa0666810 AT4G23740.1 43.243 111 58 3 17 124 500 608 3.23e-21 89.0
Msa0666810 AT4G23740.2 43.243 111 58 3 17 124 500 608 3.23e-21 89.0
Msa0666810 AT2G36570.1 42.857 126 52 4 17 124 522 645 8.59e-21 87.8
Msa0666810 AT3G17840.1 39.823 113 62 3 17 124 527 638 4.56e-20 85.9
Msa0666810 AT5G07620.1 37.500 128 76 2 1 124 229 356 2.41e-19 82.8
Msa0666810 AT5G24100.1 36.585 123 72 2 7 124 490 611 5.17e-19 82.8
Msa0666810 AT5G53320.1 38.393 112 62 3 15 126 467 571 1.87e-18 80.9
Msa0666810 AT5G63930.1 43.478 115 62 3 11 123 967 1080 6.92e-18 79.3
Msa0666810 AT5G63930.2 43.478 115 62 3 11 123 903 1016 7.37e-18 79.3
Msa0666810 AT3G02880.1 35.455 110 69 1 17 124 506 615 1.07e-17 79.0
Msa0666810 AT1G17230.2 40.984 122 69 3 4 123 954 1074 1.42e-17 78.6
Msa0666810 AT1G17230.1 40.984 122 69 3 4 123 954 1074 2.23e-17 78.2
Msa0666810 AT1G08590.1 37.984 129 76 3 1 126 878 1005 3.89e-17 77.4
Msa0666810 AT5G16590.1 36.937 111 66 2 17 124 504 613 7.06e-17 76.6
Msa0666810 AT1G34420.1 37.398 123 73 3 3 124 847 966 1.12e-16 75.9
Msa0666810 AT5G61570.1 36.434 129 69 5 1 124 239 359 1.16e-16 75.5
Msa0666810 AT5G61570.2 36.434 129 69 5 1 124 236 356 1.44e-16 75.1
Msa0666810 AT3G13380.1 40.157 127 67 4 13 136 1023 1143 6.23e-16 73.9
Msa0666810 AT3G19700.1 43.697 119 56 5 14 126 855 968 6.58e-16 73.9
Msa0666810 AT4G39400.1 39.823 113 66 2 13 124 1047 1158 8.51e-16 73.6
Msa0666810 AT3G49670.1 40.517 116 64 3 11 124 852 964 1.20e-15 73.2
Msa0666810 AT1G55610.1 40.000 115 64 3 13 124 1025 1137 1.36e-15 72.8
Msa0666810 AT1G55610.2 40.000 115 64 3 13 124 1025 1137 1.36e-15 72.8
Msa0666810 AT4G28350.1 32.773 119 75 2 5 123 508 621 4.23e-15 71.6
Msa0666810 AT3G24240.1 42.982 114 59 3 13 124 957 1066 5.37e-15 71.2
Msa0666810 AT3G02130.1 34.351 131 78 3 1 126 1021 1148 5.80e-15 71.2
Msa0666810 AT3G02130.2 34.351 131 78 3 1 126 1004 1131 6.90e-15 70.9
Msa0666810 AT5G61480.1 41.026 117 65 3 13 126 887 1002 8.47e-15 70.5
Msa0666810 AT1G34110.2 42.241 116 61 3 13 124 939 1052 1.00e-14 70.5
Msa0666810 AT1G34110.1 42.241 116 61 3 13 124 939 1052 1.04e-14 70.5
Msa0666810 AT1G78530.1 36.522 115 69 2 13 124 234 347 1.32e-14 69.7
Msa0666810 AT1G69270.1 34.351 131 78 3 1 126 410 537 2.27e-14 69.3
Msa0666810 AT2G33170.2 37.815 119 70 4 8 123 984 1101 3.08e-14 68.9
Msa0666810 AT2G33170.1 37.815 119 70 4 8 123 984 1101 3.08e-14 68.9
Msa0666810 AT2G41820.1 33.871 124 80 2 3 124 763 886 7.05e-14 68.2
Msa0666810 AT5G48940.1 36.434 129 65 5 1 124 950 1066 7.64e-14 67.8
Msa0666810 AT5G16900.4 36.134 119 66 4 11 125 548 660 9.03e-14 67.8
Msa0666810 AT5G16900.1 36.134 119 66 4 11 125 734 846 1.04e-13 67.4
Msa0666810 AT5G16900.3 36.134 119 66 4 11 125 734 846 1.04e-13 67.4
Msa0666810 AT4G31250.1 34.211 114 68 4 16 124 529 640 1.63e-13 67.0
Msa0666810 AT1G72180.1 35.484 124 73 4 13 132 849 969 1.63e-13 67.0
Msa0666810 AT1G28440.1 32.394 142 87 4 1 137 842 979 1.91e-13 66.6
Msa0666810 AT5G65700.1 36.842 114 71 1 11 124 856 968 2.98e-13 66.2
Msa0666810 AT5G65700.2 36.842 114 71 1 11 124 856 968 2.98e-13 66.2
Msa0666810 AT5G62710.1 35.652 115 65 4 13 123 475 584 4.49e-13 65.5
Msa0666810 AT5G62710.2 35.652 115 65 4 13 123 441 550 5.66e-13 65.5
Msa0666810 AT5G59270.2 33.613 119 72 3 8 122 523 638 1.85e-12 63.9
Msa0666810 AT5G56040.2 38.261 115 67 3 13 124 927 1040 2.03e-12 63.9
Msa0666810 AT4G01330.3 32.143 112 70 3 14 122 326 434 2.25e-12 63.5
Msa0666810 AT4G01330.1 32.143 112 70 3 14 122 326 434 2.51e-12 63.5
Msa0666810 AT5G59270.1 31.933 119 76 2 6 122 504 619 2.51e-12 63.5
Msa0666810 AT5G59270.3 31.933 119 76 2 6 122 507 622 2.54e-12 63.5
Msa0666810 AT4G01330.2 32.143 112 70 3 14 122 326 434 2.64e-12 63.5
Msa0666810 AT4G28650.1 35.075 134 72 5 1 126 855 981 2.96e-12 63.5
Msa0666810 AT2G27060.1 33.835 133 78 5 1 125 884 1014 3.17e-12 63.2
Msa0666810 AT3G53810.1 38.793 116 65 3 14 125 510 623 3.40e-12 63.2
Msa0666810 AT1G31420.2 35.043 117 68 5 13 125 466 578 3.66e-12 63.2
Msa0666810 AT1G31420.1 35.043 117 68 5 13 125 467 579 3.77e-12 63.2
Msa0666810 AT1G31420.3 35.043 117 68 5 13 125 467 579 3.77e-12 63.2
Msa0666810 AT4G26540.1 37.903 124 73 3 7 127 922 1044 4.02e-12 63.2
Msa0666810 AT2G35620.3 35.593 118 66 5 13 125 464 576 4.99e-12 62.8
Msa0666810 AT2G35620.1 35.593 118 66 5 13 125 464 576 4.99e-12 62.8
Msa0666810 AT2G35620.2 35.593 118 66 5 13 125 464 576 4.99e-12 62.8
Msa0666810 AT2G35620.4 35.593 118 66 5 13 125 464 576 4.99e-12 62.8
Msa0666810 AT1G75820.1 34.959 123 74 3 4 121 846 967 5.02e-12 62.8
Msa0666810 AT2G27060.3 33.835 133 78 5 1 125 791 921 5.09e-12 62.8
Msa0666810 AT1G62950.1 33.929 112 69 2 15 124 776 884 5.97e-12 62.4
Msa0666810 AT2G07040.1 33.594 128 72 5 16 136 501 622 6.26e-12 62.4
Msa0666810 AT3G55550.1 36.134 119 64 5 14 126 510 622 6.56e-12 62.4
Msa0666810 AT1G09440.1 35.714 112 66 3 14 122 321 429 7.35e-12 62.0
Msa0666810 AT1G09440.2 35.714 112 66 3 14 122 321 429 7.35e-12 62.0
Msa0666810 AT5G49770.1 34.848 132 71 5 14 138 797 920 7.79e-12 62.0
Msa0666810 AT1G12460.2 34.513 113 68 3 15 124 628 737 1.01e-11 61.6
Msa0666810 AT1G12460.1 34.513 113 68 3 15 124 767 876 1.09e-11 61.6
Msa0666810 AT3G45920.1 28.571 112 78 1 14 125 45 154 1.29e-11 59.7
Msa0666810 AT2G01210.1 38.333 120 67 6 11 127 598 713 1.35e-11 61.2
Msa0666810 AT2G02220.1 31.783 129 71 4 3 124 890 1008 1.62e-11 61.2
Msa0666810 AT5G59260.1 34.454 119 73 3 6 122 510 625 1.75e-11 61.2
Msa0666810 AT5G49660.1 33.858 127 71 5 6 126 819 938 1.82e-11 61.2
Msa0666810 AT2G31880.1 35.345 116 68 3 13 124 527 639 1.97e-11 60.8
Msa0666810 AT3G46330.1 29.204 113 78 1 13 125 730 840 2.09e-11 60.8
Msa0666810 AT3G46330.2 29.204 113 78 1 13 125 706 816 2.23e-11 60.8
Msa0666810 AT2G01950.1 32.540 126 71 2 13 124 1004 1129 2.59e-11 60.8
Msa0666810 AT1G27190.1 33.621 116 69 3 14 124 467 579 2.68e-11 60.5
Msa0666810 AT1G01540.2 32.143 112 70 3 14 122 318 426 3.32e-11 60.1
Msa0666810 AT5G58150.1 32.727 110 66 2 17 126 682 783 3.34e-11 60.5
Msa0666810 AT1G09970.1 35.043 117 69 4 13 126 844 956 3.65e-11 60.1
Msa0666810 AT2G19190.1 33.083 133 83 5 13 142 735 864 3.69e-11 60.1
Msa0666810 AT1G70110.1 34.454 119 64 5 13 124 506 617 4.32e-11 60.1
Msa0666810 AT5G07280.1 35.345 116 68 3 13 124 1080 1192 5.21e-11 59.7
Msa0666810 AT4G28490.1 33.628 113 70 3 13 124 859 967 7.26e-11 59.3
Msa0666810 AT4G04960.1 31.200 125 82 3 14 136 513 635 7.30e-11 59.3
Msa0666810 AT1G66830.1 36.607 112 61 6 18 125 577 682 8.12e-11 59.3
Msa0666810 AT3G46760.1 37.500 120 67 4 7 122 196 311 8.72e-11 58.9
Msa0666810 AT3G57830.3 35.433 127 75 5 3 124 535 659 8.81e-11 58.9
Msa0666810 AT3G57830.1 35.433 127 75 5 3 124 535 659 8.81e-11 58.9

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCCTCAGCGTTATTCTTT+TGG 0.193766 4_4:+6939294 Msa0666810:CDS
GACAAATGAAGTGTTTGATT+TGG 0.323614 4_4:+6939405 Msa0666810:CDS
ATGCCATTTGTTGGTTCTCC+TGG 0.340182 4_4:-6939340 None:intergenic
ACTTGAACTTCGACCAATAC+TGG 0.374616 4_4:-6939562 None:intergenic
AGGTTCAAACAGATGAATAA+AGG 0.376118 4_4:+6939602 Msa0666810:CDS
TGCAAAACTTGTTTAACTTC+AGG 0.401562 4_4:-6939520 None:intergenic
CATCCATTGTTAAAGAAGAA+TGG 0.404547 4_4:+6939383 Msa0666810:CDS
GGCAAGTCTATGCCATTTGT+TGG 0.409831 4_4:-6939349 None:intergenic
TTCTTTGTCTTTGAAAGCTC+CGG 0.445593 4_4:-6939247 None:intergenic
TGTCCATTCTTCTTTAACAA+TGG 0.458354 4_4:-6939386 None:intergenic
ACAATGGATGCAACATATTG+AGG 0.461356 4_4:-6939370 None:intergenic
TAAACGTCGGTCTTTGTGGT+AGG 0.487257 4_4:-6939271 None:intergenic
GCTGTAAACGTCGGTCTTTG+TGG 0.487651 4_4:-6939275 None:intergenic
TTAACTTCAGGCCTAGCAAA+AGG 0.494747 4_4:-6939508 None:intergenic
AGAGGAGATTATGCCAGTAT+TGG 0.496422 4_4:+6939549 Msa0666810:CDS
TCCTGCTGTAGCAATGATGT+TGG 0.533607 4_4:-6939206 None:intergenic
GATTTGGAACTCATGAGGGA+TGG 0.537502 4_4:+6939421 Msa0666810:CDS
CCACCAGGAGAACCAACAAA+TGG 0.539629 4_4:+6939337 Msa0666810:CDS
ACCAACATCATTGCTACAGC+AGG 0.542435 4_4:+6939205 Msa0666810:CDS
GAAGTTCAAGTTGATGATGA+TGG 0.544891 4_4:+6939574 Msa0666810:CDS
TGTAGCAATGATGTTGGTGT+TGG 0.550967 4_4:-6939200 None:intergenic
AGAAGTTCATCTCCTATCGT+TGG 0.555851 4_4:-6939445 None:intergenic
ATTGCTACAGCAGGAAGTCT+CGG 0.564926 4_4:+6939214 Msa0666810:CDS
CCATTTGTTGGTTCTCCTGG+TGG 0.568155 4_4:-6939337 None:intergenic
TGTTTGATTTGGAACTCATG+AGG 0.576156 4_4:+6939416 Msa0666810:CDS
AGTTGATGATGATGGAGCAA+AGG 0.582416 4_4:+6939582 Msa0666810:CDS
TCAGGCCTAGCAAAAGGTGA+TGG 0.589457 4_4:-6939502 None:intergenic
ATGAGGGATGGACCAACGAT+AGG 0.605785 4_4:+6939433 Msa0666810:CDS
TTAATAACTGGTAAACCACC+AGG 0.610151 4_4:+6939322 Msa0666810:CDS
GTTTGATTTGGAACTCATGA+GGG 0.628775 4_4:+6939417 Msa0666810:CDS
AAGTCTCGGATACAATGCAC+CGG 0.639160 4_4:+6939228 Msa0666810:CDS
AACGCTGAGGCTGTAAACGT+CGG 0.692624 4_4:-6939284 None:intergenic
GCTCCAAAAGAATAACGCTG+AGG 0.720184 4_4:-6939297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTGGAGCTTTTAATAAC+TGG + chr4_4:6939310-6939329 Msa0666810:CDS 30.0%
CATCCATTGTTAAAGAAGAA+TGG + chr4_4:6939383-6939402 Msa0666810:CDS 30.0%
TGTCCATTCTTCTTTAACAA+TGG - chr4_4:6939389-6939408 None:intergenic 30.0%
! GACAAATGAAGTGTTTGATT+TGG + chr4_4:6939405-6939424 Msa0666810:CDS 30.0%
! TGCAAAACTTGTTTAACTTC+AGG - chr4_4:6939523-6939542 None:intergenic 30.0%
! TTCTTTGTCTTTGAAAGCTC+CGG - chr4_4:6939250-6939269 None:intergenic 35.0%
TTAATAACTGGTAAACCACC+AGG + chr4_4:6939322-6939341 Msa0666810:CDS 35.0%
ACAATGGATGCAACATATTG+AGG - chr4_4:6939373-6939392 None:intergenic 35.0%
!! TGTTTGATTTGGAACTCATG+AGG + chr4_4:6939416-6939435 Msa0666810:CDS 35.0%
!! GTTTGATTTGGAACTCATGA+GGG + chr4_4:6939417-6939436 Msa0666810:CDS 35.0%
! ACAAGTTTTGCAACAGTTAG+AGG + chr4_4:6939531-6939550 Msa0666810:CDS 35.0%
!! GAAGTTCAAGTTGATGATGA+TGG + chr4_4:6939574-6939593 Msa0666810:CDS 35.0%
TGTAGCAATGATGTTGGTGT+TGG - chr4_4:6939203-6939222 None:intergenic 40.0%
AGAAGTTCATCTCCTATCGT+TGG - chr4_4:6939448-6939467 None:intergenic 40.0%
! TTGATCCATCACCTTTTGCT+AGG + chr4_4:6939497-6939516 Msa0666810:CDS 40.0%
TTAACTTCAGGCCTAGCAAA+AGG - chr4_4:6939511-6939530 None:intergenic 40.0%
AGAGGAGATTATGCCAGTAT+TGG + chr4_4:6939549-6939568 Msa0666810:CDS 40.0%
ACTTGAACTTCGACCAATAC+TGG - chr4_4:6939565-6939584 None:intergenic 40.0%
!! AGTTGATGATGATGGAGCAA+AGG + chr4_4:6939582-6939601 Msa0666810:CDS 40.0%
ACCAACATCATTGCTACAGC+AGG + chr4_4:6939205-6939224 Msa0666810:CDS 45.0%
TCCTGCTGTAGCAATGATGT+TGG - chr4_4:6939209-6939228 None:intergenic 45.0%
ATTGCTACAGCAGGAAGTCT+CGG + chr4_4:6939214-6939233 Msa0666810:CDS 45.0%
AAGTCTCGGATACAATGCAC+CGG + chr4_4:6939228-6939247 Msa0666810:CDS 45.0%
TAAACGTCGGTCTTTGTGGT+AGG - chr4_4:6939274-6939293 None:intergenic 45.0%
! CAGCCTCAGCGTTATTCTTT+TGG + chr4_4:6939294-6939313 Msa0666810:CDS 45.0%
GCTCCAAAAGAATAACGCTG+AGG - chr4_4:6939300-6939319 None:intergenic 45.0%
!! ATGCCATTTGTTGGTTCTCC+TGG - chr4_4:6939343-6939362 None:intergenic 45.0%
! GGCAAGTCTATGCCATTTGT+TGG - chr4_4:6939352-6939371 None:intergenic 45.0%
! GATTTGGAACTCATGAGGGA+TGG + chr4_4:6939421-6939440 Msa0666810:CDS 45.0%
GCTGTAAACGTCGGTCTTTG+TGG - chr4_4:6939278-6939297 None:intergenic 50.0%
AACGCTGAGGCTGTAAACGT+CGG - chr4_4:6939287-6939306 None:intergenic 50.0%
!! CCATTTGTTGGTTCTCCTGG+TGG - chr4_4:6939340-6939359 None:intergenic 50.0%
CCACCAGGAGAACCAACAAA+TGG + chr4_4:6939337-6939356 Msa0666810:CDS 50.0%
ATGAGGGATGGACCAACGAT+AGG + chr4_4:6939433-6939452 Msa0666810:CDS 50.0%
TCAGGCCTAGCAAAAGGTGA+TGG - chr4_4:6939505-6939524 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 6939187 6939621 6939187 ID=Msa0666810;Name=Msa0666810
chr4_4 mRNA 6939187 6939621 6939187 ID=Msa0666810-mRNA-1;Parent=Msa0666810;Name=Msa0666810-mRNA-1;_AED=0.00;_eAED=0.10;_QI=0|-1|0|1|-1|1|1|0|144
chr4_4 exon 6939187 6939621 6939187 ID=Msa0666810-mRNA-1:exon:2240;Parent=Msa0666810-mRNA-1
chr4_4 CDS 6939187 6939621 6939187 ID=Msa0666810-mRNA-1:cds;Parent=Msa0666810-mRNA-1
Gene Sequence

>Msa0666810

ATGACAACTTCAGCCAACACCAACATCATTGCTACAGCAGGAAGTCTCGGATACAATGCACCGGAGCTTTCAAAGACAAAGAAACCTACCACAAAGACCGACGTTTACAGCCTCAGCGTTATTCTTTTGGAGCTTTTAATAACTGGTAAACCACCAGGAGAACCAACAAATGGCATAGACTTGCCTCAATATGTTGCATCCATTGTTAAAGAAGAATGGACAAATGAAGTGTTTGATTTGGAACTCATGAGGGATGGACCAACGATAGGAGATGAACTTCTTAATACATTGAAATTAGCTTTGCATTGTGTTGATCCATCACCTTTTGCTAGGCCTGAAGTTAAACAAGTTTTGCAACAGTTAGAGGAGATTATGCCAGTATTGGTCGAAGTTCAAGTTGATGATGATGGAGCAAAGGTTCAAACAGATGAATAA
Protein sequence

>Msa0666810

MTTSANTNIIATAGSLGYNAPELSKTKKPTTKTDVYSLSVILLELLITGKPPGEPTNGIDLPQYVASIVKEEWTNEVFDLELMRDGPTIGDELLNTLKLALHCVDPSPFARPEVKQVLQQLEEIMPVLVEVQVDDDGAKVQTDE*