AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670730 MtrunA17_Chr4g0009901 88.321 274 32 0 1 274 1 274 0.0 506
Msa0670730 MtrunA17_Chr4g0009851 77.178 241 54 1 1 240 1 241 7.05e-134 380
Msa0670730 MtrunA17_Chr4g0009981 79.787 188 38 0 53 240 1 188 3.59e-105 306
Msa0670730 MtrunA17_Chr4g0011071 77.612 134 30 0 58 191 38 171 2.68e-74 224
Msa0670730 MtrunA17_Chr4g0009991 88.034 117 13 1 101 217 1 116 1.30e-67 206
Msa0670730 MtrunA17_Chr1g0161431 45.344 247 104 4 10 225 11 257 7.89e-66 216
Msa0670730 MtrunA17_Chr4g0067981 41.833 251 116 3 10 232 31 279 4.39e-57 194
Msa0670730 MtrunA17_Chr2g0294911 42.500 240 114 2 10 225 29 268 2.51e-56 190
Msa0670730 MtrunA17_Chr6g0471041 45.607 239 122 5 10 245 14 247 6.03e-56 185
Msa0670730 MtrunA17_Chr3g0123131 39.695 262 128 4 10 241 13 274 2.01e-54 184
Msa0670730 MtrunA17_Chr8g0376881 40.329 243 119 3 9 225 24 266 1.60e-53 181
Msa0670730 MtrunA17_Chr4g0010001 82.022 89 16 0 1 89 1 89 2.66e-50 160
Msa0670730 MtrunA17_Chr5g0423031 84.270 89 14 0 1 89 1 89 2.88e-50 163
Msa0670730 MtrunA17_Chr5g0423031 58.025 81 32 1 184 262 80 160 3.92e-17 77.4
Msa0670730 MtrunA17_Chr8g0391911 39.271 247 124 3 10 230 9 255 5.63e-49 162
Msa0670730 MtrunA17_Chr1g0149841 39.271 247 122 3 7 225 2 248 9.34e-49 168
Msa0670730 MtrunA17_Chr3g0137101 38.247 251 117 4 10 225 8 255 5.41e-47 166
Msa0670730 MtrunA17_Chr7g0275401 37.200 250 128 4 7 227 2 251 1.75e-45 154
Msa0670730 MtrunA17_Chr4g0074401 36.000 250 132 4 10 231 17 266 5.65e-44 157
Msa0670730 MtrunA17_Chr7g0275891 35.135 259 127 5 7 224 2 260 1.04e-43 151
Msa0670730 MtrunA17_Chr4g0074411 36.437 247 132 4 10 231 18 264 2.00e-43 155
Msa0670730 MtrunA17_Chr2g0304841 34.646 254 129 4 10 226 18 271 5.54e-43 155
Msa0670730 MtrunA17_Chr1g0149851 35.472 265 128 4 7 228 2 266 1.38e-41 150
Msa0670730 MtrunA17_Chr8g0383961 40.598 234 108 7 1 229 1 208 3.16e-41 142
Msa0670730 MtrunA17_Chr6g0456631 43.421 152 84 1 10 159 9 160 1.72e-39 135
Msa0670730 MtrunA17_Chr3g0134481 35.071 211 101 2 10 219 8 183 2.25e-37 130
Msa0670730 MtrunA17_Chr3g0134461 36.190 210 107 4 10 217 8 192 9.38e-36 126
Msa0670730 MtrunA17_Chr4g0074421 38.674 181 89 3 59 217 2 182 2.91e-35 125
Msa0670730 MtrunA17_Chr1g0149831 35.676 185 89 3 68 225 1 182 4.69e-28 107
Msa0670730 MtrunA17_Chr6g0473731 33.548 155 89 2 89 233 10 160 1.07e-17 82.4
Msa0670730 MtrunA17_Chr5g0417521 61.818 55 20 1 168 221 15 69 3.08e-17 80.5
Msa0670730 MtrunA17_Chr6g0461641 63.636 55 19 1 168 221 42 96 1.16e-16 79.0
Msa0670730 MtrunA17_Chr2g0321461 60.000 55 21 1 168 221 45 99 1.47e-15 75.9
Msa0670730 MtrunA17_Chr4g0031961 50.704 71 34 1 156 225 118 188 7.35e-15 73.2
Msa0670730 MtrunA17_Chr8g0350761 25.283 265 150 8 12 229 19 282 7.36e-15 73.9
Msa0670730 MtrunA17_Chr7g0261911 46.835 79 41 1 159 236 253 331 8.32e-15 73.9
Msa0670730 MtrunA17_Chr2g0324891 41.573 89 51 1 137 224 129 217 1.75e-14 72.8
Msa0670730 MtrunA17_Chr7g0255711 55.932 59 26 0 167 225 90 148 2.27e-14 71.6
Msa0670730 MtrunA17_Chr7g0274471 52.857 70 30 2 168 234 218 287 3.75e-14 72.0
Msa0670730 MtrunA17_Chr4g0042451 56.364 55 23 1 168 221 17 71 4.70e-14 71.2
Msa0670730 MtrunA17_Chr7g0254981 46.835 79 39 2 168 243 50 128 7.09e-14 70.1
Msa0670730 MtrunA17_Chr1g0198601 47.500 80 34 2 149 227 199 271 1.04e-13 70.5
Msa0670730 MtrunA17_Chr1g0190301 56.897 58 24 1 164 220 171 228 1.15e-13 70.5
Msa0670730 MtrunA17_Chr4g0040481 55.172 58 26 0 167 224 157 214 1.44e-13 70.1
Msa0670730 MtrunA17_Chr5g0400241 40.449 89 49 1 157 245 300 384 2.14e-13 69.7
Msa0670730 MtrunA17_Chr2g0283951 42.857 84 41 3 153 229 69 152 3.25e-13 68.9
Msa0670730 MtrunA17_Chr4g0062921 47.368 76 38 2 159 233 227 301 4.43e-13 68.6
Msa0670730 MtrunA17_Chr4g0054981 55.357 56 25 0 167 222 309 364 5.21e-13 68.6
Msa0670730 MtrunA17_Chr1g0162931 53.571 56 25 1 166 220 16 71 8.94e-13 67.0
Msa0670730 MtrunA17_Chr7g0243081 51.786 56 26 1 166 220 69 124 1.04e-12 67.4
Msa0670730 MtrunA17_Chr1g0175091 52.632 57 26 1 166 221 256 312 1.45e-12 67.0
Msa0670730 MtrunA17_Chr7g0258551 53.571 56 25 1 166 220 210 265 1.97e-12 66.6
Msa0670730 MtrunA17_Chr3g0095611 53.571 56 25 1 166 220 17 72 2.00e-12 65.1
Msa0670730 MtrunA17_Chr2g0291021 53.425 73 30 3 168 236 49 121 2.18e-12 66.2
Msa0670730 MtrunA17_Chr8g0356501 54.237 59 26 1 164 221 33 91 7.80e-12 64.7
Msa0670730 MtrunA17_Chr1g0210911 45.714 70 35 2 154 220 6 75 9.04e-12 64.3
Msa0670730 MtrunA17_Chr8g0379121 50.000 56 27 1 166 220 109 164 9.55e-12 64.7
Msa0670730 MtrunA17_Chr2g0310011 54.237 59 26 1 164 221 31 89 1.09e-11 64.3
Msa0670730 MtrunA17_Chr5g0422621 52.459 61 28 1 167 226 197 257 1.47e-11 63.9
Msa0670730 MtrunA17_Chr1g0197171 54.237 59 26 1 164 221 40 98 1.65e-11 63.5
Msa0670730 MtrunA17_Chr1g0175071 54.098 61 27 1 164 223 173 233 1.72e-11 63.5
Msa0670730 MtrunA17_Chr4g0045361 50.820 61 29 1 166 225 272 332 1.76e-11 63.9
Msa0670730 MtrunA17_Chr1g0175081 43.478 69 36 2 154 221 10 76 2.19e-11 59.3
Msa0670730 MtrunA17_Chr5g0409731 46.377 69 34 2 164 229 21 89 3.17e-11 62.4
Msa0670730 MtrunA17_Chr2g0320231 50.000 56 27 1 166 220 95 150 4.36e-11 62.8
Msa0670730 MtrunA17_Chr2g0288601 52.830 53 24 1 166 217 69 121 4.83e-11 59.3
Msa0670730 MtrunA17_Chr2g0290991 47.945 73 36 2 164 235 35 106 5.19e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670730 AT2G01760.1 39.544 263 120 5 7 232 4 264 1.19e-56 186
Msa0670730 AT3G16857.1 39.405 269 117 4 10 232 33 301 7.91e-52 179
Msa0670730 AT3G16857.2 39.405 269 117 4 10 232 33 301 1.28e-51 179
Msa0670730 AT2G01760.2 35.739 291 120 6 7 232 4 292 1.08e-50 172
Msa0670730 AT1G67710.1 37.329 292 145 7 11 271 8 292 1.21e-48 169
Msa0670730 AT4G31920.1 38.889 234 129 4 10 230 13 245 6.39e-46 162
Msa0670730 AT2G25180.1 36.972 284 149 7 10 268 13 291 1.19e-45 162
Msa0670730 AT3G16857.3 38.261 230 96 4 49 232 2 231 1.09e-39 146
Msa0670730 AT5G58080.1 38.272 243 118 6 10 225 14 251 5.42e-39 144
Msa0670730 AT2G01760.3 37.811 201 87 4 68 232 1 199 5.69e-37 133
Msa0670730 AT3G62670.1 32.432 222 127 6 28 226 53 274 2.11e-32 123
Msa0670730 AT1G67710.2 36.325 234 112 6 68 271 1 227 9.00e-32 122
Msa0670730 AT1G49190.1 30.000 250 140 6 10 226 30 277 2.50e-30 117
Msa0670730 AT5G49240.1 31.780 236 134 5 9 217 37 272 6.67e-30 114
Msa0670730 AT5G07210.2 31.852 270 128 9 14 229 16 283 5.92e-29 115
Msa0670730 AT5G07210.1 30.935 278 128 10 14 229 16 291 2.57e-26 108
Msa0670730 AT1G67710.3 35.047 214 102 6 88 271 12 218 2.71e-23 99.0
Msa0670730 AT2G27070.1 28.625 269 133 8 14 225 16 282 1.15e-21 94.7
Msa0670730 AT1G68210.1 26.354 277 141 7 8 225 7 279 9.12e-21 92.4
Msa0670730 AT1G49190.2 33.065 124 78 2 10 130 30 151 1.27e-17 82.8
Msa0670730 AT3G04030.1 61.818 55 20 1 168 221 45 99 3.33e-16 78.2
Msa0670730 AT3G04030.3 61.818 55 20 1 168 221 45 99 3.73e-16 78.2
Msa0670730 AT2G01060.1 49.398 83 40 2 157 238 4 85 6.49e-16 76.3
Msa0670730 AT5G18240.2 61.818 55 20 1 168 221 45 99 7.55e-16 77.0
Msa0670730 AT5G18240.3 61.818 55 20 1 168 221 45 99 7.55e-16 77.0
Msa0670730 AT5G18240.1 61.818 55 20 1 168 221 45 99 7.96e-16 77.0
Msa0670730 AT5G18240.4 61.818 55 20 1 168 221 45 99 7.96e-16 77.0
Msa0670730 AT5G29000.4 41.176 102 52 3 120 220 190 284 8.33e-16 76.3
Msa0670730 AT5G18240.5 61.818 55 20 1 168 221 45 99 8.36e-16 77.0
Msa0670730 AT5G29000.2 44.944 89 41 3 134 221 204 285 1.35e-15 76.6
Msa0670730 AT5G29000.1 45.455 88 40 3 134 220 161 241 1.36e-15 76.3
Msa0670730 AT5G29000.3 45.455 88 40 3 134 220 161 241 1.36e-15 76.3
Msa0670730 AT5G59570.2 50.649 77 35 2 149 225 125 198 3.68e-15 74.3
Msa0670730 AT5G59570.1 50.649 77 35 2 149 225 125 198 3.68e-15 74.3
Msa0670730 AT3G04030.2 57.407 54 23 0 168 221 45 98 4.12e-15 75.1
Msa0670730 AT3G46640.3 46.835 79 42 0 167 245 143 221 8.12e-15 73.6
Msa0670730 AT3G46640.2 46.835 79 42 0 167 245 143 221 9.49e-15 73.6
Msa0670730 AT3G46640.1 46.835 79 42 0 167 245 143 221 9.49e-15 73.6
Msa0670730 AT5G05090.1 55.932 59 26 0 167 225 80 138 9.76e-15 72.8
Msa0670730 AT3G10760.1 55.932 59 26 0 167 225 104 162 2.01e-14 72.8
Msa0670730 AT2G40970.1 54.237 59 27 0 167 225 104 162 2.86e-14 71.2
Msa0670730 AT5G44190.1 58.621 58 24 0 167 224 146 203 5.26e-14 71.6
Msa0670730 AT5G44190.2 58.621 58 24 0 167 224 146 203 6.17e-14 71.2
Msa0670730 AT3G04450.1 57.407 54 22 1 168 220 238 291 8.68e-14 71.2
Msa0670730 AT3G04450.4 57.407 54 22 1 168 220 198 251 9.13e-14 71.2
Msa0670730 AT3G04450.2 57.407 54 22 1 168 220 198 251 9.13e-14 71.2
Msa0670730 AT3G04450.3 57.407 54 22 1 168 220 238 291 1.16e-13 70.1
Msa0670730 AT4G28610.1 60.000 55 21 1 168 221 225 279 1.42e-13 70.5
Msa0670730 AT4G13640.1 40.594 101 41 3 168 267 37 119 3.60e-13 68.6
Msa0670730 AT4G13640.2 40.594 101 41 3 168 267 37 119 3.80e-13 68.6
Msa0670730 AT3G24120.1 38.776 98 51 2 168 256 41 138 3.87e-13 68.6
Msa0670730 AT1G69580.1 49.231 65 32 1 168 231 34 98 4.05e-13 68.9
Msa0670730 AT3G24120.2 38.776 98 51 2 168 256 41 138 4.13e-13 68.6
Msa0670730 AT1G69580.2 49.231 65 32 1 168 231 34 98 4.47e-13 68.6
Msa0670730 AT1G25550.1 48.684 76 37 2 154 227 194 269 5.35e-13 68.6
Msa0670730 AT2G20570.1 56.897 58 25 0 167 224 152 209 7.15e-13 68.6
Msa0670730 AT1G14600.1 61.538 52 19 1 170 220 25 76 1.30e-12 66.6
Msa0670730 AT2G40260.1 40.594 101 53 3 134 233 51 145 1.66e-12 67.4
Msa0670730 AT5G06800.3 58.182 55 22 1 168 221 192 246 1.87e-12 66.2
Msa0670730 AT5G06800.1 51.471 68 30 2 168 234 192 257 1.92e-12 67.0
Msa0670730 AT5G06800.2 58.182 55 22 1 168 221 192 246 3.12e-12 66.6
Msa0670730 AT4G04605.1 45.333 75 39 2 166 238 12 86 3.57e-12 64.7
Msa0670730 AT3G13040.3 53.571 56 25 1 167 221 240 295 4.02e-12 66.2
Msa0670730 AT3G13040.1 53.571 56 25 1 167 221 240 295 4.02e-12 66.2
Msa0670730 AT3G13040.2 53.571 56 25 1 167 221 240 295 4.02e-12 66.2
Msa0670730 AT1G68670.1 46.429 84 43 2 145 227 193 275 4.62e-12 65.9
Msa0670730 AT1G79430.2 42.553 94 49 2 149 237 15 108 4.83e-12 65.9
Msa0670730 AT2G20570.2 58.182 55 23 0 170 224 171 225 5.01e-12 65.9
Msa0670730 AT2G20400.2 59.259 54 21 1 168 220 231 284 8.47e-12 65.1
Msa0670730 AT2G20400.1 59.259 54 21 1 168 220 231 284 8.61e-12 65.1
Msa0670730 AT2G03500.1 50.000 62 30 1 167 227 232 293 1.60e-11 64.3
Msa0670730 AT4G18020.3 50.877 57 28 0 168 224 296 352 1.77e-11 64.7
Msa0670730 AT4G18020.2 50.877 57 28 0 168 224 296 352 1.77e-11 64.7
Msa0670730 AT4G18020.1 50.877 57 28 0 168 224 296 352 1.77e-11 64.7
Msa0670730 AT4G18020.6 50.877 57 28 0 168 224 296 352 1.77e-11 64.7
Msa0670730 AT4G18020.9 50.877 57 28 0 168 224 296 352 1.77e-11 64.7
Msa0670730 AT4G18020.8 50.877 57 28 0 168 224 296 352 1.78e-11 64.3
Msa0670730 AT4G18020.11 50.877 57 28 0 168 224 296 352 1.78e-11 64.3
Msa0670730 AT4G18020.4 50.877 57 28 0 168 224 296 352 1.78e-11 64.3
Msa0670730 AT4G18020.5 50.877 57 28 0 168 224 296 352 1.78e-11 64.3
Msa0670730 AT4G18020.10 50.877 57 28 0 168 224 296 352 1.78e-11 64.3
Msa0670730 AT4G18020.7 50.877 57 28 0 168 224 296 352 1.78e-11 64.3
Msa0670730 AT3G12730.1 52.727 55 25 1 168 221 23 77 1.82e-11 63.2
Msa0670730 AT5G45580.1 41.250 80 46 1 164 242 3 82 2.63e-11 62.8
Msa0670730 AT5G45580.2 39.080 87 52 1 157 242 12 98 2.79e-11 63.2
Msa0670730 AT2G42660.1 43.750 64 35 1 158 220 40 103 4.55e-11 62.0
Msa0670730 AT2G38300.1 48.214 56 28 1 166 220 52 107 5.23e-11 62.8

Find 65 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGCATCAACGATTTCTA+TGG 0.228648 4_4:+13483680 Msa0670730:CDS
CAAAATGATTCTCAAAATTA+TGG 0.243357 4_4:+13483827 Msa0670730:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4_4:+13483872 Msa0670730:CDS
ATCCTACCTTTACAACCTTA+TGG 0.307805 4_4:+13484161 Msa0670730:CDS
AGAAATCGTTGATGCAATTC+TGG 0.327125 4_4:-13483676 None:intergenic
CTCAAAATTATGGATGTTCC+AGG 0.335551 4_4:+13483837 Msa0670730:CDS
AATTATGGATGTTCCAGGTT+TGG 0.345656 4_4:+13483842 Msa0670730:CDS
ATTATGGATGTTCCAGGTTT+GGG 0.349053 4_4:+13483843 Msa0670730:CDS
CTAAGAATTCTTTAATTAAA+CGG 0.349796 4_4:-13484192 None:intergenic
CTTGAATTTGTTGGAAAGAA+TGG 0.358508 4_4:+13483212 Msa0670730:CDS
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4_4:+13483203 Msa0670730:CDS
AAAGTGACTAACCATGATAA+TGG 0.381195 4_4:-13483045 None:intergenic
CTGAATGGATTGATCAAAAT+CGG 0.385952 4_4:+13484097 Msa0670730:CDS
TACATATTGGACTAAGCCTT+TGG 0.386876 4_4:+13483480 Msa0670730:CDS
TCTTTAATTAAACGGCATCT+AGG 0.403700 4_4:-13484184 None:intergenic
ATCAGAAGCTGTGTTACATT+TGG 0.412873 4_4:-13483187 None:intergenic
AATGTGGGGCTTGTACATAT+TGG 0.414028 4_4:+13483467 Msa0670730:CDS
AATAAAGAATATGAAATTGT+TGG 0.416766 4_4:+13483559 Msa0670730:CDS
GGTTCAAGAAAATAGAAAAC+GGG 0.418887 4_4:+13483591 Msa0670730:CDS
CATTCTTTCCAACAAATTCA+AGG 0.419108 4_4:-13483211 None:intergenic
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4_4:+13484001 Msa0670730:intron
TGTAATCGATTCCATTATCA+TGG 0.443909 4_4:+13483034 Msa0670730:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4_4:-13483324 None:intergenic
ATATGAAATTGTTGGAAGCT+TGG 0.461542 4_4:+13483567 Msa0670730:CDS
TTGAGACCAGCAGGGAATGA+TGG 0.467536 4_4:-13482959 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.468019 4_4:-13483659 None:intergenic
AAGTCATAAGCATCCATGTT+TGG 0.471983 4_4:-13483270 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_4:+13483650 Msa0670730:CDS
AAAAGAAACACATGCAAAGA+AGG 0.478150 4_4:+13483636 Msa0670730:CDS
ACGATGACTTTCTAGCTGAA+TGG 0.481545 4_4:+13484121 Msa0670730:CDS
CACTTTCCTCAACTCTATTG+AGG 0.492388 4_4:+13483006 Msa0670730:CDS
GCAATAACCTTGAGACCAGC+AGG 0.494358 4_4:-13482968 None:intergenic
TAAACGGCATCTAGGCCATA+AGG 0.496018 4_4:-13484176 None:intergenic
GATGTTCCAGGTTTGGGTGT+AGG 0.505057 4_4:+13483849 Msa0670730:CDS
ATTCTGGCGACCAAGACAAA+CGG 0.509916 4_4:-13483660 None:intergenic
TAGGATTCATTTGAAGAGAT+CGG 0.515087 4_4:+13484020 Msa0670730:CDS
TGGTTCAAGAAAATAGAAAA+CGG 0.517102 4_4:+13483590 Msa0670730:CDS
AGGAAACAATCATACTATCA+AGG 0.517525 4_4:-13483720 None:intergenic
ATCATTCCCTGCTGGTCTCA+AGG 0.520371 4_4:+13482961 Msa0670730:CDS
TTATGAAGTCTATTCAATGT+GGG 0.525603 4_4:+13483452 Msa0670730:CDS
GTGATAGCTCTCAACCTGAA+TGG 0.533674 4_4:+13484082 Msa0670730:CDS
ACATGTCCTACACCCAAACC+TGG 0.534820 4_4:-13483855 None:intergenic
TTGACGTCTTCTTCAACCAA+AGG 0.537631 4_4:-13483496 None:intergenic
GACAACCCATCATTCCCTGC+TGG 0.539365 4_4:+13482953 Msa0670730:CDS
TGAGACCAGCAGGGAATGAT+GGG 0.539790 4_4:-13482958 None:intergenic
GAATGGATTGACTGTGGGAC+GGG 0.540651 4_4:+13484138 Msa0670730:CDS
TGGCAACATGTTGTCAGAAA+CGG 0.543020 4_4:+13483526 Msa0670730:CDS
CTATGGGCAGTCAATCAACT+AGG 0.548837 4_4:+13483697 Msa0670730:CDS
TCTAGGCCATAAGGTTGTAA+AGG 0.556671 4_4:-13484167 None:intergenic
ATTGCATCAACGATTTCTAT+GGG 0.557361 4_4:+13483681 Msa0670730:CDS
ATTATGAAGTCTATTCAATG+TGG 0.558796 4_4:+13483451 Msa0670730:CDS
TAGCTGAATGGATTGACTGT+GGG 0.565585 4_4:+13484133 Msa0670730:CDS
GAAAGTGATGTCATGATCAA+TGG 0.586165 4_4:-13482990 None:intergenic
TGAAATTGTTGGAAGCTTGG+TGG 0.586185 4_4:+13483570 Msa0670730:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4_4:-13484163 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 4_4:+13483257 Msa0670730:CDS
TATGAAGTCTATTCAATGTG+GGG 0.614025 4_4:+13483453 Msa0670730:CDS
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4_4:-13483012 None:intergenic
CTAGCTGAATGGATTGACTG+TGG 0.639966 4_4:+13484132 Msa0670730:CDS
AAGAAGACGTCAAGAGCATG+TGG 0.649026 4_4:+13483506 Msa0670730:CDS
TGAATGGATTGACTGTGGGA+CGG 0.662068 4_4:+13484137 Msa0670730:CDS
CGGCTGATGAAACAAAACAC+GGG 0.673622 4_4:+13484040 Msa0670730:CDS
CAATAACCTTGAGACCAGCA+GGG 0.699486 4_4:-13482967 None:intergenic
TCGGCTGATGAAACAAAACA+CGG 0.707340 4_4:+13484039 Msa0670730:CDS
GTTCAAGAAAATAGAAAACG+GGG 0.742093 4_4:+13483592 Msa0670730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4_4:13483559-13483578 Msa0670730:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4_4:13483828-13483847 None:intergenic 15.0%
!! AATAAAGAATATGAAATTGT+TGG + chr4_4:13483559-13483578 Msa0670730:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4_4:13483828-13483847 None:intergenic 15.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13483327-13483346 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG + chr4_4:13483405-13483424 Msa0670730:intron 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4_4:13483829-13483848 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4_4:13483827-13483846 Msa0670730:CDS 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13483327-13483346 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG + chr4_4:13483405-13483424 Msa0670730:intron 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4_4:13483829-13483848 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4_4:13483827-13483846 Msa0670730:CDS 20.0%
! ATTATGAAGTCTATTCAATG+TGG + chr4_4:13483451-13483470 Msa0670730:CDS 25.0%
! TTATGAAGTCTATTCAATGT+GGG + chr4_4:13483452-13483471 Msa0670730:CDS 25.0%
! TGGTTCAAGAAAATAGAAAA+CGG + chr4_4:13483590-13483609 Msa0670730:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4_4:13483743-13483762 None:intergenic 25.0%
! ATTATGAAGTCTATTCAATG+TGG + chr4_4:13483451-13483470 Msa0670730:CDS 25.0%
! TTATGAAGTCTATTCAATGT+GGG + chr4_4:13483452-13483471 Msa0670730:CDS 25.0%
! TGGTTCAAGAAAATAGAAAA+CGG + chr4_4:13483590-13483609 Msa0670730:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4_4:13483743-13483762 None:intergenic 25.0%
! TGTAATCGATTCCATTATCA+TGG + chr4_4:13483034-13483053 Msa0670730:CDS 30.0%
AAAGTGACTAACCATGATAA+TGG - chr4_4:13483048-13483067 None:intergenic 30.0%
CATTCTTTCCAACAAATTCA+AGG - chr4_4:13483214-13483233 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+TGG + chr4_4:13483212-13483231 Msa0670730:CDS 30.0%
!!! TGAGTTTTAGGGTAACATTT+TGG - chr4_4:13483299-13483318 None:intergenic 30.0%
TATGAAGTCTATTCAATGTG+GGG + chr4_4:13483453-13483472 Msa0670730:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4_4:13483567-13483586 Msa0670730:CDS 30.0%
GGTTCAAGAAAATAGAAAAC+GGG + chr4_4:13483591-13483610 Msa0670730:CDS 30.0%
GTTCAAGAAAATAGAAAACG+GGG + chr4_4:13483592-13483611 Msa0670730:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_4:13483636-13483655 Msa0670730:CDS 30.0%
AATTGCATCAACGATTTCTA+TGG + chr4_4:13483680-13483699 Msa0670730:CDS 30.0%
ATTGCATCAACGATTTCTAT+GGG + chr4_4:13483681-13483700 Msa0670730:CDS 30.0%
AGGAAACAATCATACTATCA+AGG - chr4_4:13483723-13483742 None:intergenic 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4_4:13483883-13483902 Msa0670730:intron 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4_4:13484001-13484020 Msa0670730:intron 30.0%
TAGGATTCATTTGAAGAGAT+CGG + chr4_4:13484020-13484039 Msa0670730:CDS 30.0%
!! CTGAATGGATTGATCAAAAT+CGG + chr4_4:13484097-13484116 Msa0670730:CDS 30.0%
! TGTAATCGATTCCATTATCA+TGG + chr4_4:13483034-13483053 Msa0670730:CDS 30.0%
AAAGTGACTAACCATGATAA+TGG - chr4_4:13483048-13483067 None:intergenic 30.0%
CATTCTTTCCAACAAATTCA+AGG - chr4_4:13483214-13483233 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+TGG + chr4_4:13483212-13483231 Msa0670730:CDS 30.0%
!!! TGAGTTTTAGGGTAACATTT+TGG - chr4_4:13483299-13483318 None:intergenic 30.0%
TATGAAGTCTATTCAATGTG+GGG + chr4_4:13483453-13483472 Msa0670730:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4_4:13483567-13483586 Msa0670730:CDS 30.0%
GGTTCAAGAAAATAGAAAAC+GGG + chr4_4:13483591-13483610 Msa0670730:CDS 30.0%
GTTCAAGAAAATAGAAAACG+GGG + chr4_4:13483592-13483611 Msa0670730:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_4:13483636-13483655 Msa0670730:CDS 30.0%
AATTGCATCAACGATTTCTA+TGG + chr4_4:13483680-13483699 Msa0670730:CDS 30.0%
ATTGCATCAACGATTTCTAT+GGG + chr4_4:13483681-13483700 Msa0670730:CDS 30.0%
AGGAAACAATCATACTATCA+AGG - chr4_4:13483723-13483742 None:intergenic 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4_4:13483883-13483902 Msa0670730:intron 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4_4:13484001-13484020 Msa0670730:intron 30.0%
TAGGATTCATTTGAAGAGAT+CGG + chr4_4:13484020-13484039 Msa0670730:CDS 30.0%
!! CTGAATGGATTGATCAAAAT+CGG + chr4_4:13484097-13484116 Msa0670730:CDS 30.0%
GAAAGTGATGTCATGATCAA+TGG - chr4_4:13482993-13483012 None:intergenic 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4_4:13483190-13483209 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_4:13483203-13483222 Msa0670730:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4_4:13483273-13483292 None:intergenic 35.0%
! GACAGGAATGTTGAGTTTTA+GGG - chr4_4:13483310-13483329 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+AGG - chr4_4:13483311-13483330 None:intergenic 35.0%
TACATATTGGACTAAGCCTT+TGG + chr4_4:13483480-13483499 Msa0670730:CDS 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_4:13483635-13483654 None:intergenic 35.0%
AGAAATCGTTGATGCAATTC+TGG - chr4_4:13483679-13483698 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+AGG + chr4_4:13483837-13483856 Msa0670730:CDS 35.0%
AATTATGGATGTTCCAGGTT+TGG + chr4_4:13483842-13483861 Msa0670730:CDS 35.0%
ATTATGGATGTTCCAGGTTT+GGG + chr4_4:13483843-13483862 Msa0670730:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4_4:13483872-13483891 Msa0670730:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4_4:13483887-13483906 Msa0670730:intron 35.0%
ACGGGAAGAAAAAAACTAAG+TGG + chr4_4:13484058-13484077 Msa0670730:CDS 35.0%
!! CGATTTTGATCAATCCATTC+AGG - chr4_4:13484099-13484118 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4_4:13484161-13484180 Msa0670730:CDS 35.0%
GAAAGTGATGTCATGATCAA+TGG - chr4_4:13482993-13483012 None:intergenic 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4_4:13483190-13483209 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_4:13483203-13483222 Msa0670730:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4_4:13483273-13483292 None:intergenic 35.0%
! GACAGGAATGTTGAGTTTTA+GGG - chr4_4:13483310-13483329 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+AGG - chr4_4:13483311-13483330 None:intergenic 35.0%
TACATATTGGACTAAGCCTT+TGG + chr4_4:13483480-13483499 Msa0670730:CDS 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_4:13483635-13483654 None:intergenic 35.0%
AGAAATCGTTGATGCAATTC+TGG - chr4_4:13483679-13483698 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+AGG + chr4_4:13483837-13483856 Msa0670730:CDS 35.0%
AATTATGGATGTTCCAGGTT+TGG + chr4_4:13483842-13483861 Msa0670730:CDS 35.0%
ATTATGGATGTTCCAGGTTT+GGG + chr4_4:13483843-13483862 Msa0670730:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4_4:13483872-13483891 Msa0670730:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4_4:13483887-13483906 Msa0670730:intron 35.0%
ACGGGAAGAAAAAAACTAAG+TGG + chr4_4:13484058-13484077 Msa0670730:CDS 35.0%
!! CGATTTTGATCAATCCATTC+AGG - chr4_4:13484099-13484118 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4_4:13484161-13484180 Msa0670730:CDS 35.0%
CACTTTCCTCAACTCTATTG+AGG + chr4_4:13483006-13483025 Msa0670730:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_4:13483015-13483034 None:intergenic 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4_4:13483257-13483276 Msa0670730:CDS 40.0%
AATGTGGGGCTTGTACATAT+TGG + chr4_4:13483467-13483486 Msa0670730:CDS 40.0%
TTGACGTCTTCTTCAACCAA+AGG - chr4_4:13483499-13483518 None:intergenic 40.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4_4:13483526-13483545 Msa0670730:CDS 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4_4:13483570-13483589 Msa0670730:CDS 40.0%
TCGGCTGATGAAACAAAACA+CGG + chr4_4:13484039-13484058 Msa0670730:CDS 40.0%
!! ACGATGACTTTCTAGCTGAA+TGG + chr4_4:13484121-13484140 Msa0670730:CDS 40.0%
!! TAGCTGAATGGATTGACTGT+GGG + chr4_4:13484133-13484152 Msa0670730:CDS 40.0%
TCTAGGCCATAAGGTTGTAA+AGG - chr4_4:13484170-13484189 None:intergenic 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4_4:13483006-13483025 Msa0670730:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_4:13483015-13483034 None:intergenic 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4_4:13483257-13483276 Msa0670730:CDS 40.0%
AATGTGGGGCTTGTACATAT+TGG + chr4_4:13483467-13483486 Msa0670730:CDS 40.0%
TTGACGTCTTCTTCAACCAA+AGG - chr4_4:13483499-13483518 None:intergenic 40.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4_4:13483526-13483545 Msa0670730:CDS 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4_4:13483570-13483589 Msa0670730:CDS 40.0%
TCGGCTGATGAAACAAAACA+CGG + chr4_4:13484039-13484058 Msa0670730:CDS 40.0%
!! ACGATGACTTTCTAGCTGAA+TGG + chr4_4:13484121-13484140 Msa0670730:CDS 40.0%
!! TAGCTGAATGGATTGACTGT+GGG + chr4_4:13484133-13484152 Msa0670730:CDS 40.0%
TCTAGGCCATAAGGTTGTAA+AGG - chr4_4:13484170-13484189 None:intergenic 40.0%
CAATAACCTTGAGACCAGCA+GGG - chr4_4:13482970-13482989 None:intergenic 45.0%
! AAGAAGACGTCAAGAGCATG+TGG + chr4_4:13483506-13483525 Msa0670730:CDS 45.0%
ATTCTGGCGACCAAGACAAA+CGG - chr4_4:13483663-13483682 None:intergenic 45.0%
CTATGGGCAGTCAATCAACT+AGG + chr4_4:13483697-13483716 Msa0670730:CDS 45.0%
CGGCTGATGAAACAAAACAC+GGG + chr4_4:13484040-13484059 Msa0670730:CDS 45.0%
GTGATAGCTCTCAACCTGAA+TGG + chr4_4:13484082-13484101 Msa0670730:CDS 45.0%
!! CTAGCTGAATGGATTGACTG+TGG + chr4_4:13484132-13484151 Msa0670730:CDS 45.0%
!! TGAATGGATTGACTGTGGGA+CGG + chr4_4:13484137-13484156 Msa0670730:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4_4:13484166-13484185 None:intergenic 45.0%
TAAACGGCATCTAGGCCATA+AGG - chr4_4:13484179-13484198 None:intergenic 45.0%
CAATAACCTTGAGACCAGCA+GGG - chr4_4:13482970-13482989 None:intergenic 45.0%
! AAGAAGACGTCAAGAGCATG+TGG + chr4_4:13483506-13483525 Msa0670730:CDS 45.0%
ATTCTGGCGACCAAGACAAA+CGG - chr4_4:13483663-13483682 None:intergenic 45.0%
CTATGGGCAGTCAATCAACT+AGG + chr4_4:13483697-13483716 Msa0670730:CDS 45.0%
CGGCTGATGAAACAAAACAC+GGG + chr4_4:13484040-13484059 Msa0670730:CDS 45.0%
GTGATAGCTCTCAACCTGAA+TGG + chr4_4:13484082-13484101 Msa0670730:CDS 45.0%
!! CTAGCTGAATGGATTGACTG+TGG + chr4_4:13484132-13484151 Msa0670730:CDS 45.0%
!! TGAATGGATTGACTGTGGGA+CGG + chr4_4:13484137-13484156 Msa0670730:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4_4:13484166-13484185 None:intergenic 45.0%
TAAACGGCATCTAGGCCATA+AGG - chr4_4:13484179-13484198 None:intergenic 45.0%
TGAGACCAGCAGGGAATGAT+GGG - chr4_4:13482961-13482980 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG - chr4_4:13482962-13482981 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG + chr4_4:13482961-13482980 Msa0670730:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG - chr4_4:13482971-13482990 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13483650-13483669 Msa0670730:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13483662-13483681 None:intergenic 50.0%
GATGTTCCAGGTTTGGGTGT+AGG + chr4_4:13483849-13483868 Msa0670730:CDS 50.0%
ACATGTCCTACACCCAAACC+TGG - chr4_4:13483858-13483877 None:intergenic 50.0%
!! GAATGGATTGACTGTGGGAC+GGG + chr4_4:13484138-13484157 Msa0670730:CDS 50.0%
TGAGACCAGCAGGGAATGAT+GGG - chr4_4:13482961-13482980 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG - chr4_4:13482962-13482981 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG + chr4_4:13482961-13482980 Msa0670730:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG - chr4_4:13482971-13482990 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13483650-13483669 Msa0670730:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13483662-13483681 None:intergenic 50.0%
GATGTTCCAGGTTTGGGTGT+AGG + chr4_4:13483849-13483868 Msa0670730:CDS 50.0%
ACATGTCCTACACCCAAACC+TGG - chr4_4:13483858-13483877 None:intergenic 50.0%
!! GAATGGATTGACTGTGGGAC+GGG + chr4_4:13484138-13484157 Msa0670730:CDS 50.0%
GACAACCCATCATTCCCTGC+TGG + chr4_4:13482953-13482972 Msa0670730:CDS 55.0%
GACAACCCATCATTCCCTGC+TGG + chr4_4:13482953-13482972 Msa0670730:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 13482938 13484205 13482938 ID=Msa0670730;Name=Msa0670730
chr4_4 mRNA 13482938 13484205 13482938 ID=Msa0670730-mRNA-1;Parent=Msa0670730;Name=Msa0670730-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.25|0.2|1|1|1|5|17|274
chr4_4 exon 13482938 13483055 13482938 ID=Msa0670730-mRNA-1:exon:4073;Parent=Msa0670730-mRNA-1
chr4_4 exon 13483184 13483333 13483184 ID=Msa0670730-mRNA-1:exon:4074;Parent=Msa0670730-mRNA-1
chr4_4 exon 13483422 13483727 13483422 ID=Msa0670730-mRNA-1:exon:4075;Parent=Msa0670730-mRNA-1
chr4_4 exon 13483817 13483893 13483817 ID=Msa0670730-mRNA-1:exon:4076;Parent=Msa0670730-mRNA-1
chr4_4 exon 13484015 13484205 13484015 ID=Msa0670730-mRNA-1:exon:4077;Parent=Msa0670730-mRNA-1
chr4_4 CDS 13482938 13483055 13482938 ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4 CDS 13483184 13483333 13483184 ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4 CDS 13483422 13483727 13483422 ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4 CDS 13483817 13483893 13483817 ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4 CDS 13484015 13484188 13484015 ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4 three_prime_UTR 13484189 13484205 13484189 ID=Msa0670730-mRNA-1:three_prime_utr;Parent=Msa0670730-mRNA-1
Gene Sequence

>Msa0670730

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCATGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAATGGACTGTTTCGATTTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTAAAACTCAACATTCCTGTCATTATGATGGCTGTTGATTTCGCAAAAAGTTCTATTATGAAGTCTATTCAATGTGGGGCTTGTACATATTGGACTAAGCCTTTGGTTGAAGAAGACGTCAAGAGCATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGATAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAATTGCATCAACGATTTCTATGGGCAGTCAATCAACTAGGCCTTGATAAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCAGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACACGGGAAGAAAAAAACTAAGTGGAGTGATAGCTCTCAACCTGAATGGATTGATCAAAATCGGAACGATGACTTTCTAGCTGAATGGATTGACTGTGGGACGGGATCCTACCTTTACAACCTTATGGCCTAG
Protein sequence

>Msa0670730

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYHVTKCNTASDALNLLERMDCFDLMLIDAHMPNMDAYDFVQNVTLKLNIPVIMMAVDFAKSSIMKSIQCGACTYWTKPLVEEDVKSMWQHVVRNGSTENKEYEIVGSLVVQENRKRGREDDNASKETHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKHGKKKTKWSDSSQPEWIDQNRNDDFLAEWIDCGTGSYLYNLMA*