AlfalfaGEDB | Msa0670730

M. sativa cultivar zhongmu-4 / Msa0670730

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670730	KEH29059.1	88.321	274	32	0	1	274	1	274	3.25e-180	506

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670730	sp\|Q8L9Y3\|ARR14_ARATH	39.544	263	120	5	7	232	4	264	1.17e-55	186

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670730	A0A072UH21	88.321	274	32	0	1	274	1	274	1.55e-180	506

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TFs/TRs:

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Gene ID	Type	Classification
Msa0670730	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670730	MtrunA17_Chr4g0009901	88.321	274	32	0	1	274	1	274	0.0	506
Msa0670730	MtrunA17_Chr4g0009851	77.178	241	54	1	1	240	1	241	7.05e-134	380
Msa0670730	MtrunA17_Chr4g0009981	79.787	188	38	0	53	240	1	188	3.59e-105	306
Msa0670730	MtrunA17_Chr4g0011071	77.612	134	30	0	58	191	38	171	2.68e-74	224
Msa0670730	MtrunA17_Chr4g0009991	88.034	117	13	1	101	217	1	116	1.30e-67	206
Msa0670730	MtrunA17_Chr1g0161431	45.344	247	104	4	10	225	11	257	7.89e-66	216
Msa0670730	MtrunA17_Chr4g0067981	41.833	251	116	3	10	232	31	279	4.39e-57	194
Msa0670730	MtrunA17_Chr2g0294911	42.500	240	114	2	10	225	29	268	2.51e-56	190
Msa0670730	MtrunA17_Chr6g0471041	45.607	239	122	5	10	245	14	247	6.03e-56	185
Msa0670730	MtrunA17_Chr3g0123131	39.695	262	128	4	10	241	13	274	2.01e-54	184

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670730	AT2G01760.1	39.544	263	120	5	7	232	4	264	1.19e-56	186
Msa0670730	AT3G16857.1	39.405	269	117	4	10	232	33	301	7.91e-52	179
Msa0670730	AT3G16857.2	39.405	269	117	4	10	232	33	301	1.28e-51	179
Msa0670730	AT2G01760.2	35.739	291	120	6	7	232	4	292	1.08e-50	172
Msa0670730	AT1G67710.1	37.329	292	145	7	11	271	8	292	1.21e-48	169
Msa0670730	AT4G31920.1	38.889	234	129	4	10	230	13	245	6.39e-46	162
Msa0670730	AT2G25180.1	36.972	284	149	7	10	268	13	291	1.19e-45	162
Msa0670730	AT3G16857.3	38.261	230	96	4	49	232	2	231	1.09e-39	146
Msa0670730	AT5G58080.1	38.272	243	118	6	10	225	14	251	5.42e-39	144
Msa0670730	AT2G01760.3	37.811	201	87	4	68	232	1	199	5.69e-37	133

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Find 65 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAACACATGCAAAGA+AGG	0.478150	4_4:+13483636	Msa0670730:CDS
AAAGTGACTAACCATGATAA+TGG	0.381195	4_4:-13483045	None:intergenic
AAGAAGACGTCAAGAGCATG+TGG	0.649026	4_4:+13483506	Msa0670730:CDS
AAGTCATAAGCATCCATGTT+TGG	0.471983	4_4:-13483270	None:intergenic
AATAAAGAATATGAAATTGT+TGG	0.416766	4_4:+13483559	Msa0670730:CDS
AATAAATTCTTACTAATGAC+AGG	0.461511	4_4:-13483324	None:intergenic
AATGTCGTTTGCAGAAATAT+AGG	0.426550	4_4:+13484001	Msa0670730:intron
AATGTGGGGCTTGTACATAT+TGG	0.414028	4_4:+13483467	Msa0670730:CDS
AATTATGGATGTTCCAGGTT+TGG	0.345656	4_4:+13483842	Msa0670730:CDS
AATTGCATCAACGATTTCTA+TGG	0.228648	4_4:+13483680	Msa0670730:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGTGACTAACCATGATAA+TGG	-	chr4_4:13483048-13483067	None:intergenic	30.0%
CATTCTTTCCAACAAATTCA+AGG	-	chr4_4:13483214-13483233	None:intergenic	30.0%
TATGAAGTCTATTCAATGTG+GGG	+	chr4_4:13483453-13483472	Msa0670730:CDS	30.0%
ATATGAAATTGTTGGAAGCT+TGG	+	chr4_4:13483567-13483586	Msa0670730:CDS	30.0%
GGTTCAAGAAAATAGAAAAC+GGG	+	chr4_4:13483591-13483610	Msa0670730:CDS	30.0%
GTTCAAGAAAATAGAAAACG+GGG	+	chr4_4:13483592-13483611	Msa0670730:CDS	30.0%
AAAAGAAACACATGCAAAGA+AGG	+	chr4_4:13483636-13483655	Msa0670730:CDS	30.0%
AATTGCATCAACGATTTCTA+TGG	+	chr4_4:13483680-13483699	Msa0670730:CDS	30.0%
ATTGCATCAACGATTTCTAT+GGG	+	chr4_4:13483681-13483700	Msa0670730:CDS	30.0%
AGGAAACAATCATACTATCA+AGG	-	chr4_4:13483723-13483742	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	13482938	13484205	13482938	ID=Msa0670730;Name=Msa0670730
chr4_4	mRNA	13482938	13484205	13482938	ID=Msa0670730-mRNA-1;Parent=Msa0670730;Name=Msa0670730-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0.25\|0.2\|1\|1\|1\|5\|17\|274
chr4_4	exon	13482938	13483055	13482938	ID=Msa0670730-mRNA-1:exon:4073;Parent=Msa0670730-mRNA-1
chr4_4	exon	13483184	13483333	13483184	ID=Msa0670730-mRNA-1:exon:4074;Parent=Msa0670730-mRNA-1
chr4_4	exon	13483422	13483727	13483422	ID=Msa0670730-mRNA-1:exon:4075;Parent=Msa0670730-mRNA-1
chr4_4	exon	13483817	13483893	13483817	ID=Msa0670730-mRNA-1:exon:4076;Parent=Msa0670730-mRNA-1
chr4_4	exon	13484015	13484205	13484015	ID=Msa0670730-mRNA-1:exon:4077;Parent=Msa0670730-mRNA-1
chr4_4	CDS	13482938	13483055	13482938	ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4	CDS	13483184	13483333	13483184	ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4	CDS	13483422	13483727	13483422	ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4	CDS	13483817	13483893	13483817	ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4	CDS	13484015	13484188	13484015	ID=Msa0670730-mRNA-1:cds;Parent=Msa0670730-mRNA-1
chr4_4	three_prime_UTR	13484189	13484205	13484189	ID=Msa0670730-mRNA-1:three_prime_utr;Parent=Msa0670730-mRNA-1

Gene Sequence

>Msa0670730

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCATGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAATGGACTGTTTCGATTTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTAAAACTCAACATTCCTGTCATTATGATGGCTGTTGATTTCGCAAAAAGTTCTATTATGAAGTCTATTCAATGTGGGGCTTGTACATATTGGACTAAGCCTTTGGTTGAAGAAGACGTCAAGAGCATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGATAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAATTGCATCAACGATTTCTATGGGCAGTCAATCAACTAGGCCTTGATAAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCAGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACACGGGAAGAAAAAAACTAAGTGGAGTGATAGCTCTCAACCTGAATGGATTGATCAAAATCGGAACGATGACTTTCTAGCTGAATGGATTGACTGTGGGACGGGATCCTACCTTTACAACCTTATGGCCTAG

Protein sequence

>Msa0670730

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYHVTKCNTASDALNLLERMDCFDLMLIDAHMPNMDAYDFVQNVTLKLNIPVIMMAVDFAKSSIMKSIQCGACTYWTKPLVEEDVKSMWQHVVRNGSTENKEYEIVGSLVVQENRKRGREDDNASKETHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKHGKKKTKWSDSSQPEWIDQNRNDDFLAEWIDCGTGSYLYNLMA*