AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670880 MtrunA17_Chr4g0009901 72.316 177 23 1 1 177 124 274 7.82e-85 251
Msa0670880 MtrunA17_Chr4g0009981 65.101 149 47 1 1 144 72 220 2.59e-57 180
Msa0670880 MtrunA17_Chr4g0009851 63.087 149 50 1 1 144 125 273 1.70e-55 177
Msa0670880 MtrunA17_Chr4g0009991 87.234 94 11 1 1 94 24 116 7.88e-53 164
Msa0670880 MtrunA17_Chr4g0011071 77.941 68 15 0 1 68 104 171 1.94e-33 117
Msa0670880 MtrunA17_Chr1g0161431 43.704 135 40 3 1 102 126 257 9.48e-25 100
Msa0670880 MtrunA17_Chr6g0471041 45.968 124 58 3 1 124 130 244 9.23e-23 93.6
Msa0670880 MtrunA17_Chr2g0294911 38.462 143 62 2 1 120 144 283 1.93e-21 90.5
Msa0670880 MtrunA17_Chr3g0123131 46.154 104 51 2 4 102 153 256 5.91e-20 86.3
Msa0670880 MtrunA17_Chr8g0376881 41.732 127 49 3 1 102 140 266 1.13e-19 85.5
Msa0670880 MtrunA17_Chr4g0067981 40.310 129 48 2 1 102 146 272 1.93e-19 84.7
Msa0670880 MtrunA17_Chr5g0417521 50.649 77 36 2 45 120 15 90 1.46e-18 81.6
Msa0670880 MtrunA17_Chr6g0461641 63.636 55 19 1 45 98 42 96 8.96e-18 79.7
Msa0670880 MtrunA17_Chr2g0304841 55.738 61 27 0 44 104 212 272 2.07e-17 79.0
Msa0670880 MtrunA17_Chr3g0137101 54.167 72 30 1 31 102 187 255 2.38e-17 79.0
Msa0670880 MtrunA17_Chr2g0321461 60.000 55 21 1 45 98 45 99 9.07e-17 76.6
Msa0670880 MtrunA17_Chr7g0275401 36.296 135 52 3 1 104 120 251 1.21e-16 75.5
Msa0670880 MtrunA17_Chr1g0149841 59.677 62 25 0 41 102 187 248 1.52e-16 76.3
Msa0670880 MtrunA17_Chr7g0261911 48.052 77 39 1 38 113 255 331 6.22e-16 74.7
Msa0670880 MtrunA17_Chr4g0074401 53.846 65 30 0 44 108 202 266 9.62e-16 73.9
Msa0670880 MtrunA17_Chr4g0074411 55.385 65 29 0 44 108 200 264 1.36e-15 73.6
Msa0670880 MtrunA17_Chr5g0423031 57.831 83 33 1 61 141 80 162 1.75e-15 70.5
Msa0670880 MtrunA17_Chr7g0274471 52.055 73 32 2 45 114 218 290 2.67e-15 72.8
Msa0670880 MtrunA17_Chr4g0031961 54.237 59 27 0 44 102 130 188 3.14e-15 72.0
Msa0670880 MtrunA17_Chr7g0255711 55.932 59 26 0 44 102 90 148 3.79e-15 71.6
Msa0670880 MtrunA17_Chr4g0042451 56.364 55 23 1 45 98 17 71 6.77e-15 71.2
Msa0670880 MtrunA17_Chr1g0190301 44.828 87 45 2 41 126 171 255 8.24e-15 71.2
Msa0670880 MtrunA17_Chr2g0324891 50.667 75 35 2 29 101 143 217 1.83e-14 70.1
Msa0670880 MtrunA17_Chr7g0254981 47.368 76 37 2 45 117 50 125 3.79e-14 68.6
Msa0670880 MtrunA17_Chr6g0473731 50.000 68 34 0 43 110 93 160 4.31e-14 69.3
Msa0670880 MtrunA17_Chr1g0162931 53.571 56 25 1 43 97 16 71 5.37e-14 68.2
Msa0670880 MtrunA17_Chr4g0040481 55.172 58 26 0 44 101 157 214 5.91e-14 68.9
Msa0670880 MtrunA17_Chr1g0198601 47.500 80 34 2 26 104 199 271 6.05e-14 68.6
Msa0670880 MtrunA17_Chr2g0283951 42.857 84 41 3 30 106 69 152 6.35e-14 68.6
Msa0670880 MtrunA17_Chr7g0243081 51.786 56 26 1 43 97 69 124 8.77e-14 68.2
Msa0670880 MtrunA17_Chr4g0054981 55.357 56 25 0 44 99 309 364 9.15e-14 68.6
Msa0670880 MtrunA17_Chr4g0074421 37.190 121 54 3 1 99 67 187 9.85e-14 66.2
Msa0670880 MtrunA17_Chr1g0175091 44.595 74 38 2 43 115 256 327 1.78e-13 67.4
Msa0670880 MtrunA17_Chr8g0356501 35.593 118 57 4 41 157 33 132 2.14e-13 67.0
Msa0670880 MtrunA17_Chr7g0258551 47.143 70 34 2 43 111 210 277 2.35e-13 67.0
Msa0670880 MtrunA17_Chr2g0291021 53.425 73 30 3 45 113 49 121 2.82e-13 66.6
Msa0670880 MtrunA17_Chr5g0400241 47.436 78 40 1 44 121 310 386 2.84e-13 67.0
Msa0670880 MtrunA17_Chr1g0210911 38.043 92 49 3 43 129 20 108 3.53e-13 66.2
Msa0670880 MtrunA17_Chr4g0062921 43.902 82 44 2 35 115 226 306 4.09e-13 66.2
Msa0670880 MtrunA17_Chr3g0095611 53.571 56 25 1 43 97 17 72 4.37e-13 64.7
Msa0670880 MtrunA17_Chr8g0379121 50.000 56 27 1 43 97 109 164 6.83e-13 65.9
Msa0670880 MtrunA17_Chr1g0149831 54.839 62 25 1 41 102 124 182 1.17e-12 63.9
Msa0670880 MtrunA17_Chr2g0310011 54.237 59 26 1 41 98 31 89 1.50e-12 64.7
Msa0670880 MtrunA17_Chr1g0175071 53.968 63 28 1 39 100 171 233 1.65e-12 64.3
Msa0670880 MtrunA17_Chr1g0197171 54.237 59 26 1 41 98 40 98 3.17e-12 63.2
Msa0670880 MtrunA17_Chr2g0320231 50.000 56 27 1 43 97 95 150 3.85e-12 63.5
Msa0670880 MtrunA17_Chr2g0290991 35.833 120 58 5 41 159 35 136 3.93e-12 63.2
Msa0670880 MtrunA17_Chr1g0175081 41.333 75 42 2 47 120 24 97 6.71e-12 59.3
Msa0670880 MtrunA17_Chr5g0409731 46.377 69 34 2 41 106 21 89 9.09e-12 62.0
Msa0670880 MtrunA17_Chr2g0288601 52.830 53 24 1 43 94 69 121 1.08e-11 59.3
Msa0670880 MtrunA17_Chr7g0275891 30.000 140 59 3 1 101 121 260 1.22e-11 62.0
Msa0670880 MtrunA17_Chr5g0422621 51.613 62 29 1 44 104 197 258 1.28e-11 62.0
Msa0670880 MtrunA17_Chr4g0045361 50.820 61 29 1 43 102 272 332 1.70e-11 61.6
Msa0670880 MtrunA17_Chr1g0149851 43.750 80 30 1 41 105 187 266 2.85e-11 61.2
Msa0670880 MtrunA17_Chr7g0242521 42.308 104 53 5 45 142 13 115 5.38e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670880 AT3G16857.2 56.410 78 32 1 25 102 219 294 3.90e-20 87.0
Msa0670880 AT3G16857.3 56.410 78 32 1 25 102 149 224 3.91e-20 87.0
Msa0670880 AT3G16857.1 56.410 78 32 1 25 102 219 294 4.51e-20 87.0
Msa0670880 AT2G01760.3 57.353 68 29 0 42 109 132 199 4.91e-20 85.5
Msa0670880 AT2G01760.1 57.353 68 29 0 42 109 197 264 9.59e-20 85.5
Msa0670880 AT2G01760.2 57.353 68 29 0 42 109 225 292 1.22e-19 85.1
Msa0670880 AT5G07210.2 50.588 85 38 1 26 106 199 283 8.46e-18 80.5
Msa0670880 AT5G07210.1 50.588 85 38 1 26 106 207 291 9.25e-18 80.5
Msa0670880 AT5G58080.1 45.133 113 52 4 31 143 181 283 1.28e-17 80.1
Msa0670880 AT5G18240.2 36.496 137 81 4 45 176 45 180 1.74e-17 79.3
Msa0670880 AT5G18240.3 36.496 137 81 4 45 176 45 180 1.74e-17 79.3
Msa0670880 AT5G18240.5 44.086 93 50 2 45 135 45 137 2.44e-17 79.0
Msa0670880 AT5G18240.1 44.086 93 50 2 45 135 45 137 2.49e-17 78.6
Msa0670880 AT5G18240.4 44.086 93 50 2 45 135 45 137 2.49e-17 78.6
Msa0670880 AT3G04030.1 61.818 55 20 1 45 98 45 99 2.51e-17 78.6
Msa0670880 AT3G04030.3 61.818 55 20 1 45 98 45 99 2.82e-17 78.6
Msa0670880 AT5G29000.1 38.655 119 65 3 5 122 155 266 6.23e-17 77.4
Msa0670880 AT5G29000.3 38.655 119 65 3 5 122 155 266 6.23e-17 77.4
Msa0670880 AT5G29000.2 38.655 119 65 3 5 122 198 309 7.29e-17 77.4
Msa0670880 AT2G01060.1 49.398 83 39 2 34 113 4 86 3.10e-16 74.7
Msa0670880 AT3G04030.2 57.407 54 23 0 45 98 45 98 3.39e-16 75.5
Msa0670880 AT5G29000.4 40.367 109 57 3 5 112 198 299 3.41e-16 75.1
Msa0670880 AT3G46640.2 49.296 71 36 0 44 114 143 213 4.03e-16 74.7
Msa0670880 AT3G46640.1 49.296 71 36 0 44 114 143 213 4.03e-16 74.7
Msa0670880 AT3G46640.3 49.296 71 36 0 44 114 143 213 4.15e-16 74.7
Msa0670880 AT5G59570.2 43.478 92 49 1 43 134 139 227 4.19e-16 74.7
Msa0670880 AT5G59570.1 43.478 92 49 1 43 134 139 227 4.19e-16 74.7
Msa0670880 AT4G31920.1 36.567 134 68 4 1 120 128 258 4.32e-16 75.5
Msa0670880 AT3G10760.1 50.000 72 34 1 31 102 93 162 1.08e-15 73.6
Msa0670880 AT5G05090.1 51.471 68 31 1 35 102 73 138 1.36e-15 72.8
Msa0670880 AT3G04450.1 50.794 63 30 1 36 97 229 291 1.55e-15 73.9
Msa0670880 AT3G04450.4 50.794 63 30 1 36 97 189 251 1.64e-15 73.6
Msa0670880 AT3G04450.2 50.794 63 30 1 36 97 189 251 1.64e-15 73.6
Msa0670880 AT3G04450.3 50.794 63 30 1 36 97 229 291 2.20e-15 72.8
Msa0670880 AT2G40970.1 54.237 59 27 0 44 102 104 162 5.81e-15 70.9
Msa0670880 AT2G25180.1 57.627 59 25 0 44 102 194 252 1.28e-14 71.2
Msa0670880 AT5G44190.1 58.621 58 24 0 44 101 146 203 1.57e-14 70.9
Msa0670880 AT4G28610.1 60.000 55 21 1 45 98 225 279 1.65e-14 70.9
Msa0670880 AT5G44190.2 58.621 58 24 0 44 101 146 203 1.94e-14 70.5
Msa0670880 AT2G27070.1 41.414 99 48 1 4 102 194 282 2.38e-14 70.5
Msa0670880 AT1G69580.1 54.545 55 24 1 45 98 34 88 5.16e-14 68.9
Msa0670880 AT1G69580.2 54.545 55 24 1 45 98 34 88 5.44e-14 68.9
Msa0670880 AT2G40260.1 49.275 69 31 2 43 110 80 145 8.46e-14 68.6
Msa0670880 AT1G67710.3 52.000 75 35 1 29 102 103 177 1.03e-13 68.6
Msa0670880 AT3G24120.2 38.462 104 58 2 45 147 41 139 1.07e-13 67.8
Msa0670880 AT3G24120.1 38.462 104 58 2 45 147 41 139 1.16e-13 67.8
Msa0670880 AT1G67710.1 52.000 75 35 1 29 102 177 251 1.41e-13 68.2
Msa0670880 AT1G67710.2 52.000 75 35 1 29 102 112 186 1.48e-13 68.2
Msa0670880 AT1G14600.1 61.538 52 19 1 47 97 25 76 1.52e-13 67.0
Msa0670880 AT2G20570.1 56.897 58 25 0 44 101 152 209 1.88e-13 67.8
Msa0670880 AT3G13040.3 51.562 64 29 2 37 98 232 295 2.72e-13 67.4
Msa0670880 AT3G13040.1 51.562 64 29 2 37 98 232 295 2.72e-13 67.4
Msa0670880 AT3G13040.2 51.562 64 29 2 37 98 232 295 2.72e-13 67.4
Msa0670880 AT1G79430.2 43.617 94 48 2 26 114 15 108 3.15e-13 67.0
Msa0670880 AT5G06800.1 51.471 68 30 2 45 111 192 257 3.79e-13 66.6
Msa0670880 AT5G45580.2 38.739 111 59 3 41 149 19 122 3.95e-13 66.2
Msa0670880 AT1G25550.1 50.685 73 34 2 39 109 202 274 4.47e-13 66.2
Msa0670880 AT5G45580.1 38.739 111 59 3 41 149 3 106 4.50e-13 65.9
Msa0670880 AT4G04605.1 47.222 72 36 2 43 112 12 83 4.97e-13 65.1
Msa0670880 AT4G13640.2 51.471 68 29 2 45 108 37 104 6.13e-13 65.9
Msa0670880 AT4G13640.1 51.471 68 29 2 45 108 37 104 6.25e-13 65.9
Msa0670880 AT5G06800.3 58.182 55 22 1 45 98 192 246 6.70e-13 65.5
Msa0670880 AT5G06800.2 58.182 55 22 1 45 98 192 246 8.65e-13 65.9
Msa0670880 AT2G20570.2 58.182 55 23 0 47 101 171 225 1.23e-12 65.5
Msa0670880 AT2G20400.2 59.259 54 21 1 45 97 231 284 2.01e-12 64.7
Msa0670880 AT2G20400.1 59.259 54 21 1 45 97 231 284 2.03e-12 64.7
Msa0670880 AT3G12730.1 50.847 59 28 1 41 98 19 77 3.50e-12 63.2
Msa0670880 AT2G38300.1 48.214 56 28 1 43 97 52 107 6.16e-12 63.2
Msa0670880 AT4G18020.3 50.877 57 28 0 45 101 296 352 6.76e-12 63.2
Msa0670880 AT4G18020.2 50.877 57 28 0 45 101 296 352 6.76e-12 63.2
Msa0670880 AT4G18020.1 50.877 57 28 0 45 101 296 352 6.76e-12 63.2
Msa0670880 AT4G18020.6 50.877 57 28 0 45 101 296 352 6.76e-12 63.2
Msa0670880 AT4G18020.9 50.877 57 28 0 45 101 296 352 6.76e-12 63.2
Msa0670880 AT2G03500.1 50.000 62 30 1 44 104 232 293 7.36e-12 63.2
Msa0670880 AT4G18020.8 50.877 57 28 0 45 101 296 352 7.45e-12 63.2
Msa0670880 AT4G18020.11 50.877 57 28 0 45 101 296 352 7.45e-12 63.2
Msa0670880 AT4G18020.4 50.877 57 28 0 45 101 296 352 7.45e-12 63.2
Msa0670880 AT4G18020.5 50.877 57 28 0 45 101 296 352 7.45e-12 63.2
Msa0670880 AT4G18020.10 50.877 57 28 0 45 101 296 352 7.45e-12 63.2
Msa0670880 AT4G18020.7 50.877 57 28 0 45 101 296 352 7.45e-12 63.2
Msa0670880 AT2G42660.1 38.462 91 49 3 46 131 51 139 9.44e-12 62.0
Msa0670880 AT2G02060.2 54.717 53 23 1 46 97 30 82 1.21e-11 61.6
Msa0670880 AT2G02060.1 54.717 53 23 1 46 97 30 82 1.21e-11 61.6
Msa0670880 AT1G68670.1 55.385 65 27 2 41 104 212 275 2.15e-11 61.6

Find 42 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGATTCTCAAAATTA+TGG 0.243357 4_4:+13735170 Msa0670880:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4_4:+13735215 Msa0670880:CDS
AACTGCATCAACGATTTCTA+TGG 0.292867 4_4:+13735023 Msa0670880:CDS
ATCCTACCTTTACAACCTTA+TGG 0.307805 4_4:+13735582 Msa0670880:CDS
CTCAAAATTATGGATGTTCC+AGG 0.335551 4_4:+13735180 Msa0670880:CDS
AATTATGGATGTTCCAGGTT+TGG 0.345656 4_4:+13735185 Msa0670880:CDS
ATTATGGATGTTCCAGGTTT+GGG 0.349053 4_4:+13735186 Msa0670880:CDS
CAGTTTGAATGTTATCGTTC+CGG 0.359832 4_4:-13735498 None:intergenic
AGAAATCGTTGATGCAGTTC+TGG 0.372917 4_4:-13735019 None:intergenic
TAGCTGAGTGGATTGATTGC+GGG 0.403037 4_4:+13735554 Msa0670880:CDS
GGTTGAAGAAAATAGAAAAC+GGG 0.411991 4_4:+13734934 Msa0670880:CDS
AATAAAGAATATGAAATTGT+TGG 0.416766 4_4:+13734902 Msa0670880:CDS
AAACTGCATATTAGGCCATA+AGG 0.423301 4_4:-13735597 None:intergenic
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4_4:+13735344 Msa0670880:intron
GAGTGGATTGATTGCGGGAC+GGG 0.433574 4_4:+13735559 Msa0670880:CDS
TTCTGGCGACCAAGACAAAC+GGG 0.460294 4_4:-13735002 None:intergenic
ATATGAAATTGTTGGAAGCT+TGG 0.461542 4_4:+13734910 Msa0670880:CDS
AAAAGATACACATGCAAAGA+AGG 0.470493 4_4:+13734979 Msa0670880:CDS
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_4:+13734993 Msa0670880:CDS
CAGCTAGAAAACCATCATTC+CGG 0.492887 4_4:-13735537 None:intergenic
GTTCTGGCGACCAAGACAAA+CGG 0.493157 4_4:-13735003 None:intergenic
TCAAATTCAATCGATCGATC+AGG 0.496009 4_4:+13735474 Msa0670880:CDS
GATGTTCCAGGTTTGGGTGT+AGG 0.505057 4_4:+13735192 Msa0670880:CDS
CTAGCTGAGTGGATTGATTG+CGG 0.505708 4_4:+13735553 Msa0670880:CDS
TGGTTGAAGAAAATAGAAAA+CGG 0.506154 4_4:+13734933 Msa0670880:CDS
TAGGATTCATTTGAAGAGAT+CGG 0.515087 4_4:+13735363 Msa0670880:CDS
ACTGATCACGACCGGAATGA+TGG 0.518029 4_4:+13735526 Msa0670880:CDS
CTATGGGCTGTCAATCAACT+AGG 0.518108 4_4:+13735040 Msa0670880:CDS
AGGAAAGAAGCATACTATCA+AGG 0.524721 4_4:-13735063 None:intergenic
ATTAGGCCATAAGGTTGTAA+AGG 0.531488 4_4:-13735588 None:intergenic
ACATGTCCTACACCCAAACC+TGG 0.534820 4_4:-13735198 None:intergenic
TGGCAACATGTTGTCAGAAA+CGG 0.543020 4_4:+13734869 Msa0670880:CDS
TTCAATCGATCGATCAGGAC+CGG 0.553886 4_4:+13735479 Msa0670880:CDS
CTGAGCTGACTGATCACGAC+CGG 0.562799 4_4:+13735518 Msa0670880:CDS
TGAAATTGTTGGAAGCTTGG+TGG 0.576315 4_4:+13734913 Msa0670880:CDS
ACTGCATCAACGATTTCTAT+GGG 0.587946 4_4:+13735024 Msa0670880:CDS
TCAAGACGAGTGCATTGACA+AGG 0.590781 4_4:+13735435 Msa0670880:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4_4:-13735584 None:intergenic
TGAGTGGATTGATTGCGGGA+CGG 0.641804 4_4:+13735558 Msa0670880:CDS
CGGCTGATGAAACAAAACGT+GGG 0.694912 4_4:+13735383 Msa0670880:CDS
TCGGCTGATGAAACAAAACG+TGG 0.707531 4_4:+13735382 Msa0670880:CDS
GTTGAAGAAAATAGAAAACG+GGG 0.712341 4_4:+13734935 Msa0670880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4_4:13734902-13734921 Msa0670880:CDS 15.0%
!! AAAAATAAGTGAGATAAAAA+TGG - chr4_4:13735137-13735156 None:intergenic 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4_4:13735171-13735190 None:intergenic 15.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4_4:13735172-13735191 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4_4:13735170-13735189 Msa0670880:CDS 20.0%
! TGGTTGAAGAAAATAGAAAA+CGG + chr4_4:13734933-13734952 Msa0670880:CDS 25.0%
! ACAAAGCAAAGAAATGAATT+AGG - chr4_4:13735086-13735105 None:intergenic 25.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4_4:13734910-13734929 Msa0670880:CDS 30.0%
GGTTGAAGAAAATAGAAAAC+GGG + chr4_4:13734934-13734953 Msa0670880:CDS 30.0%
GTTGAAGAAAATAGAAAACG+GGG + chr4_4:13734935-13734954 Msa0670880:CDS 30.0%
! TTTGCATGTGTATCTTTTGA+GGG - chr4_4:13734977-13734996 None:intergenic 30.0%
AAAAGATACACATGCAAAGA+AGG + chr4_4:13734979-13734998 Msa0670880:CDS 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4_4:13735226-13735245 Msa0670880:intron 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4_4:13735344-13735363 Msa0670880:intron 30.0%
TAGGATTCATTTGAAGAGAT+CGG + chr4_4:13735363-13735382 Msa0670880:CDS 30.0%
! TTGCATGTGTATCTTTTGAG+GGG - chr4_4:13734976-13734995 None:intergenic 35.0%
! CTTTGCATGTGTATCTTTTG+AGG - chr4_4:13734978-13734997 None:intergenic 35.0%
AACTGCATCAACGATTTCTA+TGG + chr4_4:13735023-13735042 Msa0670880:CDS 35.0%
ACTGCATCAACGATTTCTAT+GGG + chr4_4:13735024-13735043 Msa0670880:CDS 35.0%
! AGGAAAGAAGCATACTATCA+AGG - chr4_4:13735066-13735085 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+AGG + chr4_4:13735180-13735199 Msa0670880:CDS 35.0%
AATTATGGATGTTCCAGGTT+TGG + chr4_4:13735185-13735204 Msa0670880:CDS 35.0%
ATTATGGATGTTCCAGGTTT+GGG + chr4_4:13735186-13735205 Msa0670880:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4_4:13735215-13735234 Msa0670880:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4_4:13735230-13735249 Msa0670880:intron 35.0%
GTGGGAAGAAAAAATCAAAG+TGG + chr4_4:13735401-13735420 Msa0670880:CDS 35.0%
TCAAATTCAATCGATCGATC+AGG + chr4_4:13735474-13735493 Msa0670880:CDS 35.0%
CAGTTTGAATGTTATCGTTC+CGG - chr4_4:13735501-13735520 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4_4:13735582-13735601 Msa0670880:CDS 35.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4_4:13734869-13734888 Msa0670880:CDS 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4_4:13734913-13734932 Msa0670880:CDS 40.0%
AGAAATCGTTGATGCAGTTC+TGG - chr4_4:13735022-13735041 None:intergenic 40.0%
CAGCTAGAAAACCATCATTC+CGG - chr4_4:13735540-13735559 None:intergenic 40.0%
!!! ATGATGGTTTTCTAGCTGAG+TGG + chr4_4:13735542-13735561 Msa0670880:CDS 40.0%
! CTATGGGCTGTCAATCAACT+AGG + chr4_4:13735040-13735059 Msa0670880:CDS 45.0%
TCGGCTGATGAAACAAAACG+TGG + chr4_4:13735382-13735401 Msa0670880:CDS 45.0%
CGGCTGATGAAACAAAACGT+GGG + chr4_4:13735383-13735402 Msa0670880:CDS 45.0%
! TCAAGACGAGTGCATTGACA+AGG + chr4_4:13735435-13735454 Msa0670880:CDS 45.0%
TTCAATCGATCGATCAGGAC+CGG + chr4_4:13735479-13735498 Msa0670880:CDS 45.0%
!! CTAGCTGAGTGGATTGATTG+CGG + chr4_4:13735553-13735572 Msa0670880:CDS 45.0%
!! TAGCTGAGTGGATTGATTGC+GGG + chr4_4:13735554-13735573 Msa0670880:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4_4:13735587-13735606 None:intergenic 45.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13734993-13735012 Msa0670880:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13735005-13735024 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_4:13735006-13735025 None:intergenic 50.0%
GATGTTCCAGGTTTGGGTGT+AGG + chr4_4:13735192-13735211 Msa0670880:CDS 50.0%
ACATGTCCTACACCCAAACC+TGG - chr4_4:13735201-13735220 None:intergenic 50.0%
ACTGATCACGACCGGAATGA+TGG + chr4_4:13735526-13735545 Msa0670880:CDS 50.0%
!! TGAGTGGATTGATTGCGGGA+CGG + chr4_4:13735558-13735577 Msa0670880:CDS 50.0%
CTGAGCTGACTGATCACGAC+CGG + chr4_4:13735518-13735537 Msa0670880:CDS 55.0%
!! GAGTGGATTGATTGCGGGAC+GGG + chr4_4:13735559-13735578 Msa0670880:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 13734866 13735609 13734866 ID=Msa0670880;Name=Msa0670880
chr4_4 mRNA 13734866 13735609 13734866 ID=Msa0670880-mRNA-1;Parent=Msa0670880;Name=Msa0670880-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|1|1|3|0|177
chr4_4 exon 13734866 13735070 13734866 ID=Msa0670880-mRNA-1:exon:4192;Parent=Msa0670880-mRNA-1
chr4_4 exon 13735160 13735236 13735160 ID=Msa0670880-mRNA-1:exon:4193;Parent=Msa0670880-mRNA-1
chr4_4 exon 13735358 13735609 13735358 ID=Msa0670880-mRNA-1:exon:4194;Parent=Msa0670880-mRNA-1
chr4_4 CDS 13734866 13735070 13734866 ID=Msa0670880-mRNA-1:cds;Parent=Msa0670880-mRNA-1
chr4_4 CDS 13735160 13735236 13735160 ID=Msa0670880-mRNA-1:cds;Parent=Msa0670880-mRNA-1
chr4_4 CDS 13735358 13735609 13735358 ID=Msa0670880-mRNA-1:cds;Parent=Msa0670880-mRNA-1
Gene Sequence

>Msa0670880

ATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTGAAGAAAATAGAAAACGGGGTAGAGAAGATGATAACCCCTCAAAAGATACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAACTGCATCAACGATTTCTATGGGCTGTCAATCAACTAGGCCTTGATAAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCAGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACGTGGGAAGAAAAAATCAAAGTGGAGTGATAGCTTTCAAGACGAGTGCATTGACAAGGTTCATAGTGATAGCTTTCAAATTCAATCGATCGATCAGGACCGGAACGATAACATTCAAACTGAGCTGACTGATCACGACCGGAATGATGGTTTTCTAGCTGAGTGGATTGATTGCGGGACGGGATCCTACCTTTACAACCTTATGGCCTAA
Protein sequence

>Msa0670880

MWQHVVRNGSTENKEYEIVGSLVVEENRKRGREDDNPSKDTHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKRGKKKSKWSDSFQDECIDKVHSDSFQIQSIDQDRNDNIQTELTDHDRNDGFLAEWIDCGTGSYLYNLMA*