AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670890 MtrunA17_Chr4g0009851 82.484 314 52 2 1 313 1 312 0.0 538
Msa0670890 MtrunA17_Chr4g0009981 85.057 261 37 1 53 313 1 259 7.32e-167 464
Msa0670890 MtrunA17_Chr4g0009901 78.926 242 49 1 1 242 1 240 8.55e-135 383
Msa0670890 MtrunA17_Chr4g0011071 79.856 139 26 1 55 193 35 171 3.96e-77 233
Msa0670890 MtrunA17_Chr1g0161431 45.565 248 102 5 10 226 11 256 2.91e-67 221
Msa0670890 MtrunA17_Chr6g0471041 45.051 293 142 8 10 296 14 293 8.96e-67 214
Msa0670890 MtrunA17_Chr8g0376881 40.769 260 124 5 6 239 21 276 1.24e-57 194
Msa0670890 MtrunA17_Chr2g0294911 39.850 266 132 4 10 249 29 292 1.84e-57 195
Msa0670890 MtrunA17_Chr4g0009991 78.151 119 23 2 101 219 1 116 3.18e-57 180
Msa0670890 MtrunA17_Chr3g0123131 40.984 244 114 4 10 225 13 254 4.17e-57 193
Msa0670890 MtrunA17_Chr4g0067981 41.152 243 115 3 10 226 31 271 1.24e-56 194
Msa0670890 MtrunA17_Chr1g0149841 39.922 258 127 5 7 236 2 259 2.40e-53 182
Msa0670890 MtrunA17_Chr4g0010001 86.517 89 12 0 1 89 1 89 1.18e-52 167
Msa0670890 MtrunA17_Chr8g0391911 40.083 242 117 4 4 219 3 242 1.93e-51 170
Msa0670890 MtrunA17_Chr7g0275401 37.891 256 126 4 7 232 2 254 2.25e-50 169
Msa0670890 MtrunA17_Chr5g0423031 86.517 89 12 0 1 89 1 89 4.42e-50 164
Msa0670890 MtrunA17_Chr3g0137101 33.754 317 156 8 10 293 8 303 7.24e-49 172
Msa0670890 MtrunA17_Chr7g0275891 35.379 277 138 5 7 242 2 278 4.44e-48 164
Msa0670890 MtrunA17_Chr2g0304841 35.686 255 129 4 10 229 18 272 1.11e-46 167
Msa0670890 MtrunA17_Chr1g0149851 36.398 261 125 5 7 226 2 262 3.73e-44 158
Msa0670890 MtrunA17_Chr4g0074401 33.865 251 138 4 10 233 17 266 4.93e-44 158
Msa0670890 MtrunA17_Chr4g0074411 35.743 249 135 4 9 233 17 264 1.03e-43 157
Msa0670890 MtrunA17_Chr8g0383961 40.433 277 123 11 1 265 1 247 1.17e-43 150
Msa0670890 MtrunA17_Chr6g0456631 44.375 160 85 2 4 161 3 160 4.84e-42 143
Msa0670890 MtrunA17_Chr3g0134461 37.264 212 104 4 10 219 8 192 2.21e-39 137
Msa0670890 MtrunA17_Chr3g0134481 35.211 213 100 3 10 221 8 183 4.23e-38 133
Msa0670890 MtrunA17_Chr4g0074421 36.813 182 93 3 59 219 2 182 7.36e-37 130
Msa0670890 MtrunA17_Chr1g0149831 38.265 196 91 5 68 236 1 193 3.09e-33 122
Msa0670890 MtrunA17_Chr6g0473731 30.978 184 107 3 89 259 10 186 2.60e-21 93.6
Msa0670890 MtrunA17_Chr8g0350761 24.242 363 213 14 6 309 15 374 5.80e-20 90.1
Msa0670890 MtrunA17_Chr7g0223971 28.846 208 90 5 91 249 1 199 3.27e-19 87.8
Msa0670890 MtrunA17_Chr5g0417521 59.259 54 21 1 170 222 15 68 1.30e-15 76.6
Msa0670890 MtrunA17_Chr2g0324891 36.937 111 66 3 118 226 109 217 1.34e-15 77.0
Msa0670890 MtrunA17_Chr6g0461641 59.259 54 21 1 170 222 42 95 6.51e-15 74.7
Msa0670890 MtrunA17_Chr4g0040481 50.000 58 29 0 169 226 157 214 1.30e-14 73.9
Msa0670890 MtrunA17_Chr7g0255711 55.172 58 26 0 169 226 90 147 1.33e-14 73.2
Msa0670890 MtrunA17_Chr4g0031961 53.448 58 27 0 169 226 130 187 1.92e-14 72.8
Msa0670890 MtrunA17_Chr2g0321461 57.407 54 22 1 170 222 45 98 2.51e-14 72.8
Msa0670890 MtrunA17_Chr7g0254981 41.837 98 54 2 161 257 41 136 3.98e-14 71.2
Msa0670890 MtrunA17_Chr7g0261911 46.512 86 43 3 161 245 253 336 2.17e-13 70.5
Msa0670890 MtrunA17_Chr4g0054981 37.349 83 52 0 169 251 309 391 3.90e-13 69.7
Msa0670890 MtrunA17_Chr5g0400241 37.349 83 52 0 169 251 310 392 4.11e-13 69.7
Msa0670890 MtrunA17_Chr5g0400241 29.508 122 81 3 10 129 14 132 9.47e-11 62.4
Msa0670890 MtrunA17_Chr4g0042451 53.704 54 24 1 170 222 17 70 7.58e-13 68.2
Msa0670890 MtrunA17_Chr7g0274471 53.846 65 27 2 170 231 218 282 1.15e-12 68.2
Msa0670890 MtrunA17_Chr2g0291021 56.522 69 25 3 170 233 49 117 1.76e-12 67.0
Msa0670890 MtrunA17_Chr1g0190301 53.448 58 26 1 166 222 171 228 3.24e-12 66.6
Msa0670890 MtrunA17_Chr8g0356501 42.697 89 49 2 170 256 37 125 1.09e-11 64.7
Msa0670890 MtrunA17_Chr1g0162931 41.772 79 44 2 156 233 5 82 1.39e-11 63.9
Msa0670890 MtrunA17_Chr7g0243081 51.786 56 26 1 168 222 69 124 2.41e-11 63.9
Msa0670890 MtrunA17_Chr7g0242521 54.286 70 23 4 157 222 5 69 2.84e-11 62.4
Msa0670890 MtrunA17_Chr3g0095611 49.123 57 28 1 168 223 17 73 3.09e-11 62.0
Msa0670890 MtrunA17_Chr6g0479431 47.541 61 31 1 163 222 195 255 6.19e-11 62.8
Msa0670890 MtrunA17_Chr1g0198601 46.575 73 31 2 151 222 199 264 9.39e-11 62.0
Msa0670890 MtrunA17_Chr3g0109981 39.759 83 49 1 141 222 245 327 9.81e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670890 AT2G01760.1 40.076 262 116 7 7 231 4 261 7.03e-59 194
Msa0670890 AT1G67710.1 40.000 265 127 6 11 243 8 272 3.31e-54 185
Msa0670890 AT2G01760.2 36.207 290 116 8 7 231 4 289 6.86e-53 179
Msa0670890 AT3G16857.1 38.043 276 125 7 10 239 33 308 2.41e-52 182
Msa0670890 AT3G16857.2 38.043 276 125 7 10 239 33 308 2.42e-52 182
Msa0670890 AT4G31920.1 36.546 249 145 3 10 248 13 258 2.95e-49 172
Msa0670890 AT2G25180.1 39.506 243 117 4 10 226 13 251 2.22e-48 171
Msa0670890 AT5G58080.1 38.800 250 127 6 10 236 14 260 7.32e-42 153
Msa0670890 AT3G16857.3 36.709 237 104 7 49 239 2 238 3.25e-40 149
Msa0670890 AT2G01760.3 38.000 200 84 6 68 231 1 196 4.74e-38 137
Msa0670890 AT1G67710.2 39.130 207 95 5 68 243 1 207 2.00e-36 136
Msa0670890 AT5G49240.1 32.203 236 135 5 9 219 37 272 3.84e-34 126
Msa0670890 AT1G49190.1 26.061 330 203 8 10 305 30 352 1.84e-31 122
Msa0670890 AT3G62670.1 31.481 216 127 5 28 222 53 268 1.32e-30 120
Msa0670890 AT5G07210.2 27.778 270 141 6 14 231 16 283 2.56e-27 112
Msa0670890 AT1G67710.3 37.968 187 85 5 88 243 12 198 2.79e-27 110
Msa0670890 AT5G07210.1 26.978 278 141 7 14 231 16 291 7.06e-25 105
Msa0670890 AT2G27070.1 24.460 278 153 7 14 236 16 291 2.34e-20 92.0
Msa0670890 AT1G49190.2 33.871 124 81 1 10 132 30 153 1.52e-19 89.4
Msa0670890 AT5G29000.4 35.862 145 75 6 103 238 166 301 8.66e-16 77.0
Msa0670890 AT2G01060.1 50.602 83 38 2 159 238 4 86 1.13e-15 76.3
Msa0670890 AT5G29000.1 33.728 169 88 7 103 256 123 282 1.17e-15 77.0
Msa0670890 AT5G29000.3 33.728 169 88 7 103 256 123 282 1.17e-15 77.0
Msa0670890 AT5G29000.2 34.868 152 81 6 103 245 166 308 1.19e-15 77.4
Msa0670890 AT5G59570.2 49.296 71 33 1 156 226 130 197 1.71e-15 75.9
Msa0670890 AT5G59570.1 49.296 71 33 1 156 226 130 197 1.71e-15 75.9
Msa0670890 AT3G04030.1 59.259 54 21 1 170 222 45 98 7.44e-15 75.1
Msa0670890 AT2G40970.1 53.448 58 27 0 169 226 104 161 8.25e-15 73.2
Msa0670890 AT3G04030.3 59.259 54 21 1 170 222 45 98 8.80e-15 74.7
Msa0670890 AT5G05090.1 55.172 58 26 0 169 226 80 137 8.96e-15 73.6
Msa0670890 AT5G18240.2 52.113 71 32 2 153 222 29 98 1.05e-14 74.7
Msa0670890 AT5G18240.3 52.113 71 32 2 153 222 29 98 1.05e-14 74.7
Msa0670890 AT5G44190.1 53.448 58 27 0 169 226 146 203 1.09e-14 74.3
Msa0670890 AT3G10760.1 55.172 58 26 0 169 226 104 161 1.13e-14 73.9
Msa0670890 AT5G18240.1 52.113 71 32 2 153 222 29 98 1.14e-14 74.3
Msa0670890 AT5G18240.4 52.113 71 32 2 153 222 29 98 1.14e-14 74.3
Msa0670890 AT5G18240.5 52.113 71 32 2 153 222 29 98 1.17e-14 74.3
Msa0670890 AT5G44190.2 53.448 58 27 0 169 226 146 203 1.38e-14 73.9
Msa0670890 AT3G46640.3 53.448 58 27 0 169 226 143 200 1.98e-14 73.2
Msa0670890 AT3G46640.2 53.448 58 27 0 169 226 143 200 2.34e-14 72.8
Msa0670890 AT3G46640.1 53.448 58 27 0 169 226 143 200 2.34e-14 72.8
Msa0670890 AT3G04030.2 56.604 53 23 0 170 222 45 97 5.11e-14 72.4
Msa0670890 AT2G20570.1 51.724 58 28 0 169 226 152 209 9.06e-14 72.0
Msa0670890 AT4G28610.1 61.111 54 20 1 170 222 225 278 4.11e-13 69.7
Msa0670890 AT3G04450.3 55.556 54 23 1 170 222 238 291 4.76e-13 68.9
Msa0670890 AT2G20570.2 52.727 55 26 0 172 226 171 225 6.49e-13 69.3
Msa0670890 AT4G18020.8 39.474 76 43 1 152 227 281 353 7.33e-13 69.3
Msa0670890 AT4G18020.11 39.474 76 43 1 152 227 281 353 7.33e-13 69.3
Msa0670890 AT4G18020.4 39.474 76 43 1 152 227 281 353 7.33e-13 69.3
Msa0670890 AT4G18020.5 39.474 76 43 1 152 227 281 353 7.33e-13 69.3
Msa0670890 AT4G18020.10 39.474 76 43 1 152 227 281 353 7.33e-13 69.3
Msa0670890 AT4G18020.7 39.474 76 43 1 152 227 281 353 7.33e-13 69.3
Msa0670890 AT3G04450.1 55.556 54 23 1 170 222 238 291 7.41e-13 69.3
Msa0670890 AT3G04450.4 55.556 54 23 1 170 222 198 251 7.47e-13 68.9
Msa0670890 AT3G04450.2 55.556 54 23 1 170 222 198 251 7.47e-13 68.9
Msa0670890 AT4G18020.3 39.474 76 43 1 152 227 281 353 7.88e-13 69.3
Msa0670890 AT4G18020.2 39.474 76 43 1 152 227 281 353 7.88e-13 69.3
Msa0670890 AT4G18020.1 39.474 76 43 1 152 227 281 353 7.88e-13 69.3
Msa0670890 AT4G18020.6 39.474 76 43 1 152 227 281 353 7.88e-13 69.3
Msa0670890 AT4G18020.9 39.474 76 43 1 152 227 281 353 7.88e-13 69.3
Msa0670890 AT1G69580.1 51.852 54 25 1 170 222 34 87 9.28e-12 65.5
Msa0670890 AT1G69580.2 51.852 54 25 1 170 222 34 87 9.41e-12 65.5
Msa0670890 AT1G25550.1 47.826 69 34 2 156 222 194 262 1.61e-11 64.7
Msa0670890 AT1G14600.1 56.604 53 22 1 172 223 25 77 2.50e-11 63.2
Msa0670890 AT3G24120.1 50.000 68 30 2 170 233 41 108 3.26e-11 63.5
Msa0670890 AT3G24120.2 50.000 68 30 2 170 233 41 108 3.33e-11 63.5
Msa0670890 AT3G13040.3 47.059 68 33 2 169 233 240 307 4.78e-11 63.5
Msa0670890 AT3G13040.1 47.059 68 33 2 169 233 240 307 4.78e-11 63.5
Msa0670890 AT3G13040.2 47.059 68 33 2 169 233 240 307 4.78e-11 63.5
Msa0670890 AT2G20400.2 57.407 54 22 1 170 222 231 284 9.50e-11 62.8
Msa0670890 AT2G20400.1 57.407 54 22 1 170 222 231 284 9.74e-11 62.8

Find 54 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGTTGCCAGCCATTTAC+AGG 0.283862 4_4:+13742058 Msa0670890:CDS
TGCTAAAACGAACCTGTAAA+TGG 0.306750 4_4:-13742070 None:intergenic
GACATTGTGTGGTCGAATTC+AGG 0.320772 4_4:-13742578 None:intergenic
CTTGAATTTGTTGGAAAGAA+AGG 0.326280 4_4:+13741360 Msa0670890:CDS
AGAAACCGCTGATGCAGTTC+TGG 0.337779 4_4:-13741859 None:intergenic
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4_4:+13741351 Msa0670890:CDS
TGAGTTGTAGGGTAACATTT+TGG 0.376494 4_4:-13741444 None:intergenic
CACATATTGGACTAAGCCTT+TGG 0.377509 4_4:+13741657 Msa0670890:CDS
GATAAATCATCATCAACATA+TGG 0.380393 4_4:-13742690 None:intergenic
CTTTCTTTCCAACAAATTCA+AGG 0.396613 4_4:-13741359 None:intergenic
AATAAAGAATATAAAATTGT+TGG 0.400605 4_4:+13741742 Msa0670890:CDS
ATCAGAAGCTGTATTGCATT+TGG 0.403201 4_4:-13741335 None:intergenic
TGACAGGAATGTTGAGTTGT+AGG 0.408636 4_4:-13741456 None:intergenic
ACGCTAAATTGTGTTTCAAA+AGG 0.431875 4_4:-13742513 None:intergenic
TTGAGACCGGCTGGGAATGA+TGG 0.453544 4_4:-13741095 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.460294 4_4:-13741842 None:intergenic
AATAAATTCTTACTAATGAC+AGG 0.461511 4_4:-13741472 None:intergenic
TGTAATCGATATCATTATCA+AGG 0.463829 4_4:+13741170 Msa0670890:CDS
AATGTGGGGCTTGCACATAT+TGG 0.467947 4_4:+13741644 Msa0670890:CDS
TCAACATATGGGAACAAGTT+GGG 0.468173 4_4:-13742678 None:intergenic
AAGTCATAAGCATCCATGTT+TGG 0.471983 4_4:-13741418 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_4:+13741833 Msa0670890:CDS
GCAATAACCTTGAGACCGGC+TGG 0.475576 4_4:-13741104 None:intergenic
AAAAGAAACACATGCAAAGA+AGG 0.478150 4_4:+13741819 Msa0670890:CDS
GTTCTGGCGACCAAGACAAA+CGG 0.493157 4_4:-13741843 None:intergenic
CTATATGCTGTCAATCAACT+AGG 0.499504 4_4:+13741880 Msa0670890:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4_4:-13741903 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4_4:+13741628 Msa0670890:CDS
ATATAAAATTGTTGGAAGCT+CGG 0.513354 4_4:+13741750 Msa0670890:CDS
GACAGGAATGTTGAGTTGTA+GGG 0.519779 4_4:-13741455 None:intergenic
TTATGAAGTCTATTGAATGT+GGG 0.532174 4_4:+13741629 Msa0670890:CDS
AAAACGAACCTGTAAATGGC+TGG 0.535893 4_4:-13742066 None:intergenic
AGAAAACTCTGGTCTTGCTC+CGG 0.537353 4_4:-13742555 None:intergenic
CACTGTCCTCAACTCTATTG+AGG 0.546156 4_4:+13741142 Msa0670890:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4_4:-13742689 None:intergenic
ATCATTCCCAGCCGGTCTCA+AGG 0.552068 4_4:+13741097 Msa0670890:CDS
ATCAACATATGGGAACAAGT+TGG 0.563419 4_4:-13742679 None:intergenic
AGAAGCTACTCACTCAATGC+CGG 0.575449 4_4:+13742536 Msa0670890:CDS
TGAGACCGGCTGGGAATGAT+GGG 0.577892 4_4:-13741094 None:intergenic
CTTCATAATAGAACTTGCTG+TGG 0.579779 4_4:-13741614 None:intergenic
AATTGCAATAACCTTGAGAC+CGG 0.581075 4_4:-13741108 None:intergenic
CGACACACGACAACAATGGA+AGG 0.598655 4_4:+13742611 Msa0670890:CDS
TATGAAGTCTATTGAATGTG+GGG 0.598969 4_4:+13741630 Msa0670890:CDS
TCGAATTCAGGAGAAAACTC+TGG 0.600958 4_4:-13742566 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 4_4:+13741405 Msa0670890:CDS
GGTCGCCAGAACTGCATCAG+CGG 0.609761 4_4:+13741854 Msa0670890:CDS
CAATAACCTTGAGACCGGCT+GGG 0.613963 4_4:-13741103 None:intergenic
CACAACCCATCATTCCCAGC+CGG 0.618478 4_4:+13741089 Msa0670890:CDS
ATTGCGACACACGACAACAA+TGG 0.629205 4_4:+13742607 Msa0670890:CDS
TCTTACTACATGTTGCCACA+TGG 0.630006 4_4:-13741698 None:intergenic
ACATCTCCTCAATAGAGTTG+AGG 0.637933 4_4:-13741148 None:intergenic
GCAATTGTCATGACATTGTG+TGG 0.655655 4_4:-13742589 None:intergenic
TAAAATTGTTGGAAGCTCGG+TGG 0.661387 4_4:+13741753 Msa0670890:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4_4:+13742703 Msa0670890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATAAAATTGT+TGG + chr4_4:13741742-13741761 Msa0670890:CDS 10.0%
!! AATAAAGAATATAAAATTGT+TGG + chr4_4:13741742-13741761 Msa0670890:CDS 10.0%
!! CATATAGTAAAAAAGTAATT+AGG - chr4_4:13741926-13741945 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4_4:13742376-13742395 Msa0670890:intron 15.0%
!! CATATAGTAAAAAAGTAATT+AGG - chr4_4:13741926-13741945 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4_4:13742376-13742395 Msa0670890:intron 15.0%
!! AGAAACATTAGAATTGAATA+AGG - chr4_4:13741217-13741236 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13741475-13741494 None:intergenic 20.0%
!! TGGTTCAAAAAAATAGAAAA+CGG + chr4_4:13741773-13741792 Msa0670890:CDS 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4_4:13741981-13742000 Msa0670890:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4_4:13741982-13742001 Msa0670890:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4_4:13742014-13742033 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4_4:13742013-13742032 Msa0670890:CDS 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG - chr4_4:13742045-13742064 None:intergenic 20.0%
!! AAAAATGTTGTTAGTAATGT+CGG - chr4_4:13742649-13742668 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4_4:13742692-13742711 None:intergenic 20.0%
!! AGAAACATTAGAATTGAATA+AGG - chr4_4:13741217-13741236 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13741475-13741494 None:intergenic 20.0%
!! TGGTTCAAAAAAATAGAAAA+CGG + chr4_4:13741773-13741792 Msa0670890:CDS 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4_4:13741981-13742000 Msa0670890:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4_4:13741982-13742001 Msa0670890:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4_4:13742014-13742033 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4_4:13742013-13742032 Msa0670890:CDS 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG - chr4_4:13742045-13742064 None:intergenic 20.0%
!! AAAAATGTTGTTAGTAATGT+CGG - chr4_4:13742649-13742668 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4_4:13742692-13742711 None:intergenic 20.0%
! TGTAATCGATATCATTATCA+AGG + chr4_4:13741170-13741189 Msa0670890:CDS 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_4:13741582-13741601 Msa0670890:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_4:13741628-13741647 Msa0670890:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_4:13741629-13741648 Msa0670890:CDS 25.0%
! ATATAAAATTGTTGGAAGCT+CGG + chr4_4:13741750-13741769 Msa0670890:CDS 25.0%
! GGTTCAAAAAAATAGAAAAC+GGG + chr4_4:13741774-13741793 Msa0670890:CDS 25.0%
! GTTCAAAAAAATAGAAAACG+GGG + chr4_4:13741775-13741794 Msa0670890:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4_4:13741983-13742002 Msa0670890:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG - chr4_4:13742044-13742063 None:intergenic 25.0%
! AAAATTACAACAACAGAGTT+AGG - chr4_4:13742170-13742189 None:intergenic 25.0%
! TTTAATAACATGCAAGACTA+AGG - chr4_4:13742216-13742235 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG - chr4_4:13742340-13742359 None:intergenic 25.0%
! GTAGAATGAAAAAATCAAAG+AGG + chr4_4:13742487-13742506 Msa0670890:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4_4:13742693-13742712 None:intergenic 25.0%
! TGTAATCGATATCATTATCA+AGG + chr4_4:13741170-13741189 Msa0670890:CDS 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_4:13741582-13741601 Msa0670890:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_4:13741628-13741647 Msa0670890:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_4:13741629-13741648 Msa0670890:CDS 25.0%
! ATATAAAATTGTTGGAAGCT+CGG + chr4_4:13741750-13741769 Msa0670890:CDS 25.0%
! GGTTCAAAAAAATAGAAAAC+GGG + chr4_4:13741774-13741793 Msa0670890:CDS 25.0%
! GTTCAAAAAAATAGAAAACG+GGG + chr4_4:13741775-13741794 Msa0670890:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4_4:13741983-13742002 Msa0670890:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG - chr4_4:13742044-13742063 None:intergenic 25.0%
! AAAATTACAACAACAGAGTT+AGG - chr4_4:13742170-13742189 None:intergenic 25.0%
! TTTAATAACATGCAAGACTA+AGG - chr4_4:13742216-13742235 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG - chr4_4:13742340-13742359 None:intergenic 25.0%
! GTAGAATGAAAAAATCAAAG+AGG + chr4_4:13742487-13742506 Msa0670890:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4_4:13742693-13742712 None:intergenic 25.0%
CTTTCTTTCCAACAAATTCA+AGG - chr4_4:13741362-13741381 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+AGG + chr4_4:13741360-13741379 Msa0670890:CDS 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_4:13741630-13741649 Msa0670890:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_4:13741819-13741838 Msa0670890:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_4:13741906-13741925 None:intergenic 30.0%
!!! CATTCTACCTTTTTTCTCTT+CGG - chr4_4:13742475-13742494 None:intergenic 30.0%
ACGCTAAATTGTGTTTCAAA+AGG - chr4_4:13742516-13742535 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4_4:13742703-13742722 Msa0670890:CDS 30.0%
CTTTCTTTCCAACAAATTCA+AGG - chr4_4:13741362-13741381 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+AGG + chr4_4:13741360-13741379 Msa0670890:CDS 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_4:13741630-13741649 Msa0670890:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_4:13741819-13741838 Msa0670890:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_4:13741906-13741925 None:intergenic 30.0%
!!! CATTCTACCTTTTTTCTCTT+CGG - chr4_4:13742475-13742494 None:intergenic 30.0%
ACGCTAAATTGTGTTTCAAA+AGG - chr4_4:13742516-13742535 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4_4:13742703-13742722 Msa0670890:CDS 30.0%
AATTGCAATAACCTTGAGAC+CGG - chr4_4:13741111-13741130 None:intergenic 35.0%
ATCAGAAGCTGTATTGCATT+TGG - chr4_4:13741338-13741357 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_4:13741351-13741370 Msa0670890:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4_4:13741421-13741440 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4_4:13741447-13741466 None:intergenic 35.0%
!! CTTCATAATAGAACTTGCTG+TGG - chr4_4:13741617-13741636 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4_4:13741669-13741688 Msa0670890:CDS 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4_4:13741676-13741695 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_4:13741818-13741837 None:intergenic 35.0%
CTATATGCTGTCAATCAACT+AGG + chr4_4:13741880-13741899 Msa0670890:CDS 35.0%
TGCTAAAACGAACCTGTAAA+TGG - chr4_4:13742073-13742092 None:intergenic 35.0%
AAAAGATCACTGACAATCCT+AGG - chr4_4:13742146-13742165 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4_4:13742252-13742271 Msa0670890:intron 35.0%
AGAGGATACATAGAAGCTAA+TGG - chr4_4:13742322-13742341 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4_4:13742681-13742700 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4_4:13742682-13742701 None:intergenic 35.0%
AATTGCAATAACCTTGAGAC+CGG - chr4_4:13741111-13741130 None:intergenic 35.0%
ATCAGAAGCTGTATTGCATT+TGG - chr4_4:13741338-13741357 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_4:13741351-13741370 Msa0670890:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4_4:13741421-13741440 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4_4:13741447-13741466 None:intergenic 35.0%
!! CTTCATAATAGAACTTGCTG+TGG - chr4_4:13741617-13741636 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4_4:13741669-13741688 Msa0670890:CDS 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4_4:13741676-13741695 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_4:13741818-13741837 None:intergenic 35.0%
CTATATGCTGTCAATCAACT+AGG + chr4_4:13741880-13741899 Msa0670890:CDS 35.0%
TGCTAAAACGAACCTGTAAA+TGG - chr4_4:13742073-13742092 None:intergenic 35.0%
AAAAGATCACTGACAATCCT+AGG - chr4_4:13742146-13742165 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4_4:13742252-13742271 Msa0670890:intron 35.0%
AGAGGATACATAGAAGCTAA+TGG - chr4_4:13742322-13742341 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4_4:13742681-13742700 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4_4:13742682-13742701 None:intergenic 35.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_4:13741151-13741170 None:intergenic 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4_4:13741405-13741424 Msa0670890:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4_4:13741458-13741477 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4_4:13741459-13741478 None:intergenic 40.0%
CACATATTGGACTAAGCCTT+TGG + chr4_4:13741657-13741676 Msa0670890:CDS 40.0%
AAAAAGAGGTCAAGACCATG+TGG + chr4_4:13741683-13741702 Msa0670890:CDS 40.0%
TCTTACTACATGTTGCCACA+TGG - chr4_4:13741701-13741720 None:intergenic 40.0%
TAAAATTGTTGGAAGCTCGG+TGG + chr4_4:13741753-13741772 Msa0670890:CDS 40.0%
AAAACGAACCTGTAAATGGC+TGG - chr4_4:13742069-13742088 None:intergenic 40.0%
AATCCTAGGCAACTAGACTT+AGG - chr4_4:13742132-13742151 None:intergenic 40.0%
AGCTCAACCGAAGAGAAAAA+AGG + chr4_4:13742465-13742484 Msa0670890:CDS 40.0%
TCGAATTCAGGAGAAAACTC+TGG - chr4_4:13742569-13742588 None:intergenic 40.0%
GCAATTGTCATGACATTGTG+TGG - chr4_4:13742592-13742611 None:intergenic 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_4:13741151-13741170 None:intergenic 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4_4:13741405-13741424 Msa0670890:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4_4:13741458-13741477 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4_4:13741459-13741478 None:intergenic 40.0%
CACATATTGGACTAAGCCTT+TGG + chr4_4:13741657-13741676 Msa0670890:CDS 40.0%
AAAAAGAGGTCAAGACCATG+TGG + chr4_4:13741683-13741702 Msa0670890:CDS 40.0%
TCTTACTACATGTTGCCACA+TGG - chr4_4:13741701-13741720 None:intergenic 40.0%
TAAAATTGTTGGAAGCTCGG+TGG + chr4_4:13741753-13741772 Msa0670890:CDS 40.0%
AAAACGAACCTGTAAATGGC+TGG - chr4_4:13742069-13742088 None:intergenic 40.0%
AATCCTAGGCAACTAGACTT+AGG - chr4_4:13742132-13742151 None:intergenic 40.0%
AGCTCAACCGAAGAGAAAAA+AGG + chr4_4:13742465-13742484 Msa0670890:CDS 40.0%
TCGAATTCAGGAGAAAACTC+TGG - chr4_4:13742569-13742588 None:intergenic 40.0%
GCAATTGTCATGACATTGTG+TGG - chr4_4:13742592-13742611 None:intergenic 40.0%
CACTGTCCTCAACTCTATTG+AGG + chr4_4:13741142-13741161 Msa0670890:CDS 45.0%
AATGTGGGGCTTGCACATAT+TGG + chr4_4:13741644-13741663 Msa0670890:CDS 45.0%
ACATGTTGCCAGCCATTTAC+AGG + chr4_4:13742058-13742077 Msa0670890:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG + chr4_4:13742126-13742145 Msa0670890:intron 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4_4:13742536-13742555 Msa0670890:CDS 45.0%
!! AGAAAACTCTGGTCTTGCTC+CGG - chr4_4:13742558-13742577 None:intergenic 45.0%
GACATTGTGTGGTCGAATTC+AGG - chr4_4:13742581-13742600 None:intergenic 45.0%
ATTGCGACACACGACAACAA+TGG + chr4_4:13742607-13742626 Msa0670890:CDS 45.0%
CACTGTCCTCAACTCTATTG+AGG + chr4_4:13741142-13741161 Msa0670890:CDS 45.0%
AATGTGGGGCTTGCACATAT+TGG + chr4_4:13741644-13741663 Msa0670890:CDS 45.0%
ACATGTTGCCAGCCATTTAC+AGG + chr4_4:13742058-13742077 Msa0670890:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG + chr4_4:13742126-13742145 Msa0670890:intron 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4_4:13742536-13742555 Msa0670890:CDS 45.0%
!! AGAAAACTCTGGTCTTGCTC+CGG - chr4_4:13742558-13742577 None:intergenic 45.0%
GACATTGTGTGGTCGAATTC+AGG - chr4_4:13742581-13742600 None:intergenic 45.0%
ATTGCGACACACGACAACAA+TGG + chr4_4:13742607-13742626 Msa0670890:CDS 45.0%
CAATAACCTTGAGACCGGCT+GGG - chr4_4:13741106-13741125 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13741833-13741852 Msa0670890:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13741845-13741864 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_4:13741846-13741865 None:intergenic 50.0%
AGAAACCGCTGATGCAGTTC+TGG - chr4_4:13741862-13741881 None:intergenic 50.0%
CGACACACGACAACAATGGA+AGG + chr4_4:13742611-13742630 Msa0670890:CDS 50.0%
CAATAACCTTGAGACCGGCT+GGG - chr4_4:13741106-13741125 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13741833-13741852 Msa0670890:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13741845-13741864 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_4:13741846-13741865 None:intergenic 50.0%
AGAAACCGCTGATGCAGTTC+TGG - chr4_4:13741862-13741881 None:intergenic 50.0%
CGACACACGACAACAATGGA+AGG + chr4_4:13742611-13742630 Msa0670890:CDS 50.0%
CACAACCCATCATTCCCAGC+CGG + chr4_4:13741089-13741108 Msa0670890:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4_4:13741097-13741116 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4_4:13741098-13741117 None:intergenic 55.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4_4:13741097-13741116 Msa0670890:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4_4:13741107-13741126 None:intergenic 55.0%
CACAACCCATCATTCCCAGC+CGG + chr4_4:13741089-13741108 Msa0670890:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4_4:13741097-13741116 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4_4:13741098-13741117 None:intergenic 55.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4_4:13741097-13741116 Msa0670890:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4_4:13741107-13741126 None:intergenic 55.0%
GGTCGCCAGAACTGCATCAG+CGG + chr4_4:13741854-13741873 Msa0670890:CDS 60.0%
GGTCGCCAGAACTGCATCAG+CGG + chr4_4:13741854-13741873 Msa0670890:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 13741074 13742728 13741074 ID=Msa0670890;Name=Msa0670890
chr4_4 mRNA 13741074 13742728 13741074 ID=Msa0670890-mRNA-1;Parent=Msa0670890;Name=Msa0670890-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0|1|1|1|5|0|313
chr4_4 exon 13741074 13741191 13741074 ID=Msa0670890-mRNA-1:exon:4177;Parent=Msa0670890-mRNA-1
chr4_4 exon 13741332 13741481 13741332 ID=Msa0670890-mRNA-1:exon:4178;Parent=Msa0670890-mRNA-1
chr4_4 exon 13741599 13741910 13741599 ID=Msa0670890-mRNA-1:exon:4179;Parent=Msa0670890-mRNA-1
chr4_4 exon 13742003 13742079 13742003 ID=Msa0670890-mRNA-1:exon:4180;Parent=Msa0670890-mRNA-1
chr4_4 exon 13742444 13742728 13742444 ID=Msa0670890-mRNA-1:exon:4181;Parent=Msa0670890-mRNA-1
chr4_4 CDS 13741074 13741191 13741074 ID=Msa0670890-mRNA-1:cds;Parent=Msa0670890-mRNA-1
chr4_4 CDS 13741332 13741481 13741332 ID=Msa0670890-mRNA-1:cds;Parent=Msa0670890-mRNA-1
chr4_4 CDS 13741599 13741910 13741599 ID=Msa0670890-mRNA-1:cds;Parent=Msa0670890-mRNA-1
chr4_4 CDS 13742003 13742079 13742003 ID=Msa0670890-mRNA-1:cds;Parent=Msa0670890-mRNA-1
chr4_4 CDS 13742444 13742728 13742444 ID=Msa0670890-mRNA-1:cds;Parent=Msa0670890-mRNA-1
Gene Sequence

>Msa0670890

ATGGCAGTTTTCACTCACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTGTCCTCAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGTTACCAAATGCAATACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAGCAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCACATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGACCATGTGGCAACATGTAGTAAGAATTAGAAACTGTTTGACTGAAAATAAAGAATATAAAATTGTTGGAAGCTCGGTGGTTCAAAAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAACTGCATCAGCGGTTTCTATATGCTGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGAAGTATAGAAATTATTTAAAGAGCTCAACCGAAGAGAAAAAAGGTAGAATGAAAAAATCAAAGAGGAGTCCTTTTGAAACACAATTTAGCGTAGAAGCTACTCACTCAATGCCGGAGCAAGACCAGAGTTTTCTCCTGAATTCGACCACACAATGTCATGACAATTGCGACACACGACAACAATGGAAGGAAATTAGTGAATCCGACATTACTAACAACATTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>Msa0670890

MAVFTHNPSFPAGLKVIAIDHDITVLNSIEEMCNRYHYQVTKCNTASDALNLLERKDCFDVMLIDAHMPNMDAYDFVQNVTLQLNIPVIMMAVDSTASSIMKSIECGACTYWTKPLVEKEVKTMWQHVVRIRNCLTENKEYKIVGSSVVQKNRKRGREDANASKETHAKKARLSWSPELHQRFLYAVNQLGLDRATPKKILKIMDVPDMKKEHVASHLQKYRNYLKSSTEEKKGRMKKSKRSPFETQFSVEATHSMPEQDQSFLLNSTTQCHDNCDTRQQWKEISESDITNNIFSDLPNLFPYVDDDLSSLVW*