AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670900 MtrunA17_Chr4g0009901 76.580 269 37 1 1 269 32 274 4.47e-148 415
Msa0670900 MtrunA17_Chr4g0009851 69.565 230 70 0 1 230 33 262 1.68e-111 323
Msa0670900 MtrunA17_Chr4g0009981 71.770 209 59 0 22 230 1 209 1.09e-102 299
Msa0670900 MtrunA17_Chr4g0011071 74.627 134 34 0 27 160 38 171 4.17e-72 219
Msa0670900 MtrunA17_Chr4g0009991 83.761 117 18 1 70 186 1 116 2.10e-64 197
Msa0670900 MtrunA17_Chr1g0161431 44.298 228 90 4 1 194 33 257 3.41e-55 187
Msa0670900 MtrunA17_Chr6g0471041 46.226 212 103 5 7 216 42 244 1.04e-50 171
Msa0670900 MtrunA17_Chr2g0294911 40.435 230 110 3 7 212 57 283 1.54e-47 166
Msa0670900 MtrunA17_Chr4g0067981 43.721 215 93 4 7 194 59 272 3.97e-47 166
Msa0670900 MtrunA17_Chr3g0123131 40.090 222 105 4 1 194 35 256 1.02e-44 158
Msa0670900 MtrunA17_Chr8g0376881 40.455 220 105 4 1 194 47 266 1.59e-44 157
Msa0670900 MtrunA17_Chr7g0275401 37.838 222 103 4 7 196 33 251 8.02e-41 142
Msa0670900 MtrunA17_Chr3g0137101 37.991 229 104 5 1 194 30 255 1.24e-39 145
Msa0670900 MtrunA17_Chr1g0149841 38.288 222 109 4 1 194 27 248 1.21e-38 141
Msa0670900 MtrunA17_Chr8g0391911 37.668 223 113 3 3 199 33 255 1.10e-37 133
Msa0670900 MtrunA17_Chr4g0074401 35.526 228 116 5 1 200 42 266 4.41e-36 135
Msa0670900 MtrunA17_Chr2g0304841 35.242 227 110 4 7 196 46 272 5.34e-36 135
Msa0670900 MtrunA17_Chr4g0074411 36.073 219 115 4 7 200 46 264 1.24e-35 134
Msa0670900 MtrunA17_Chr7g0275891 32.906 234 116 4 1 193 27 260 3.13e-35 129
Msa0670900 MtrunA17_Chr4g0074421 38.674 181 89 3 28 186 2 182 3.38e-34 122
Msa0670900 MtrunA17_Chr1g0149851 33.750 240 116 4 1 197 27 266 1.83e-33 127
Msa0670900 MtrunA17_Chr3g0134481 34.783 184 84 2 6 188 35 183 2.15e-32 117
Msa0670900 MtrunA17_Chr8g0383961 40.206 194 89 6 6 198 41 208 2.46e-31 117
Msa0670900 MtrunA17_Chr6g0456631 41.727 139 78 2 3 138 33 171 4.52e-30 111
Msa0670900 MtrunA17_Chr4g0010001 82.759 58 10 0 1 58 32 89 5.20e-30 108
Msa0670900 MtrunA17_Chr5g0423031 84.483 58 9 0 1 58 32 89 8.22e-29 108
Msa0670900 MtrunA17_Chr5g0423031 54.118 85 37 1 153 235 80 164 1.38e-15 73.2
Msa0670900 MtrunA17_Chr3g0134461 35.714 182 90 4 7 186 36 192 4.61e-27 103
Msa0670900 MtrunA17_Chr1g0149831 35.135 185 90 3 37 194 1 182 3.11e-26 102
Msa0670900 MtrunA17_Chr6g0473731 34.194 155 88 2 58 202 10 160 7.71e-18 82.8
Msa0670900 MtrunA17_Chr5g0417521 61.818 55 20 1 137 190 15 69 2.27e-17 80.9
Msa0670900 MtrunA17_Chr6g0461641 63.636 55 19 1 137 190 42 96 5.98e-17 79.7
Msa0670900 MtrunA17_Chr2g0321461 60.000 55 21 1 137 190 45 99 7.99e-16 76.6
Msa0670900 MtrunA17_Chr4g0031961 41.837 98 51 3 99 194 95 188 5.38e-15 73.6
Msa0670900 MtrunA17_Chr7g0261911 46.753 77 40 1 130 205 255 331 9.31e-15 73.6
Msa0670900 MtrunA17_Chr7g0255711 55.932 59 26 0 136 194 90 148 2.00e-14 71.6
Msa0670900 MtrunA17_Chr7g0274471 50.685 73 33 2 137 206 218 290 2.92e-14 72.0
Msa0670900 MtrunA17_Chr4g0042451 56.364 55 23 1 137 190 17 71 3.18e-14 71.6
Msa0670900 MtrunA17_Chr2g0324891 45.055 91 44 3 121 205 143 233 4.67e-14 71.6
Msa0670900 MtrunA17_Chr1g0190301 44.828 87 45 2 133 218 171 255 5.48e-14 71.2
Msa0670900 MtrunA17_Chr4g0040481 47.297 74 35 1 136 205 157 230 1.47e-13 70.1
Msa0670900 MtrunA17_Chr7g0254981 56.364 55 23 1 137 190 50 104 2.34e-13 68.6
Msa0670900 MtrunA17_Chr5g0400241 48.718 78 39 1 136 213 310 386 2.56e-13 69.7
Msa0670900 MtrunA17_Chr2g0283951 42.857 84 41 3 122 198 69 152 3.26e-13 68.9
Msa0670900 MtrunA17_Chr4g0054981 55.357 56 25 0 136 191 309 364 4.68e-13 68.9
Msa0670900 MtrunA17_Chr1g0162931 53.571 56 25 1 135 189 16 71 5.80e-13 67.4
Msa0670900 MtrunA17_Chr7g0243081 51.786 56 26 1 135 189 69 124 7.00e-13 67.8
Msa0670900 MtrunA17_Chr8g0356501 35.593 118 57 4 133 249 33 132 7.67e-13 67.4
Msa0670900 MtrunA17_Chr1g0198601 35.556 135 70 5 70 196 146 271 9.51e-13 67.4
Msa0670900 MtrunA17_Chr1g0210911 39.560 91 47 3 135 220 20 107 1.33e-12 66.6
Msa0670900 MtrunA17_Chr1g0175091 52.632 57 26 1 135 190 256 312 1.68e-12 67.0
Msa0670900 MtrunA17_Chr3g0095611 53.571 56 25 1 135 189 17 72 1.71e-12 65.1
Msa0670900 MtrunA17_Chr7g0258551 53.571 56 25 1 135 189 210 265 1.81e-12 66.6
Msa0670900 MtrunA17_Chr4g0062921 46.753 77 39 2 127 202 226 301 2.17e-12 66.6
Msa0670900 MtrunA17_Chr2g0291021 52.055 73 31 3 137 205 49 121 2.21e-12 66.2
Msa0670900 MtrunA17_Chr2g0310011 35.593 118 57 4 133 249 31 130 4.12e-12 65.5
Msa0670900 MtrunA17_Chr8g0379121 50.000 56 27 1 135 189 109 164 6.32e-12 65.1
Msa0670900 MtrunA17_Chr1g0175071 53.968 63 28 1 131 192 171 233 9.81e-12 64.3
Msa0670900 MtrunA17_Chr1g0197171 54.237 59 26 1 133 190 40 98 1.55e-11 63.5
Msa0670900 MtrunA17_Chr2g0288601 41.975 81 37 2 116 186 41 121 1.72e-11 60.5
Msa0670900 MtrunA17_Chr5g0422621 33.043 115 72 2 83 196 148 258 2.11e-11 63.5
Msa0670900 MtrunA17_Chr5g0409731 46.377 69 34 2 133 198 21 89 3.18e-11 62.4
Msa0670900 MtrunA17_Chr2g0320231 50.000 56 27 1 135 189 95 150 3.21e-11 62.8
Msa0670900 MtrunA17_Chr1g0175081 49.057 53 26 1 139 190 24 76 3.43e-11 58.9
Msa0670900 MtrunA17_Chr4g0045361 50.820 61 29 1 135 194 272 332 4.10e-11 62.8
Msa0670900 MtrunA17_Chr2g0290991 47.945 73 36 2 133 204 35 106 6.23e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670900 AT2G01760.1 39.496 238 105 4 1 201 29 264 2.76e-47 162
Msa0670900 AT2G01760.2 39.224 232 102 4 7 201 63 292 4.26e-45 157
Msa0670900 AT3G16857.1 40.598 234 93 4 7 194 61 294 1.71e-43 157
Msa0670900 AT3G16857.2 40.598 234 93 4 7 194 61 294 2.36e-43 156
Msa0670900 AT1G67710.1 39.910 223 105 4 1 194 29 251 6.66e-41 148
Msa0670900 AT4G31920.1 37.885 227 123 5 1 212 35 258 9.67e-40 145
Msa0670900 AT3G16857.3 39.910 223 88 5 18 194 2 224 8.21e-39 143
Msa0670900 AT2G25180.1 38.889 216 100 4 7 194 41 252 2.39e-36 136
Msa0670900 AT2G01760.3 37.313 201 88 3 37 201 1 199 7.66e-36 130
Msa0670900 AT5G58080.1 37.339 233 110 8 7 212 42 265 4.54e-35 133
Msa0670900 AT1G67710.2 39.785 186 84 3 37 194 1 186 1.65e-31 121
Msa0670900 AT3G62670.1 32.110 218 125 6 1 195 57 274 6.92e-30 116
Msa0670900 AT1G49190.1 28.571 245 138 7 1 212 52 292 4.11e-26 106
Msa0670900 AT5G07210.2 30.952 252 118 7 1 198 34 283 4.85e-25 104
Msa0670900 AT5G07210.1 31.915 235 104 7 18 198 59 291 3.85e-24 101
Msa0670900 AT5G49240.1 30.952 210 118 3 4 186 63 272 2.09e-23 97.1
Msa0670900 AT1G67710.3 38.554 166 74 3 57 194 12 177 3.80e-23 98.2
Msa0670900 AT2G27070.1 29.134 254 121 8 21 221 58 305 5.08e-20 89.7
Msa0670900 AT3G04030.1 61.818 55 20 1 137 190 45 99 1.96e-16 78.6
Msa0670900 AT3G04030.3 61.818 55 20 1 137 190 45 99 2.27e-16 78.6
Msa0670900 AT5G18240.2 61.818 55 20 1 137 190 45 99 4.51e-16 77.8
Msa0670900 AT5G18240.3 61.818 55 20 1 137 190 45 99 4.51e-16 77.8
Msa0670900 AT5G18240.1 61.818 55 20 1 137 190 45 99 5.09e-16 77.4
Msa0670900 AT5G18240.4 61.818 55 20 1 137 190 45 99 5.09e-16 77.4
Msa0670900 AT5G18240.5 61.818 55 20 1 137 190 45 99 5.14e-16 77.4
Msa0670900 AT5G59570.2 41.237 97 54 1 101 194 102 198 1.56e-15 75.5
Msa0670900 AT5G59570.1 41.237 97 54 1 101 194 102 198 1.56e-15 75.5
Msa0670900 AT3G04030.2 57.407 54 23 0 137 190 45 98 2.50e-15 75.5
Msa0670900 AT5G29000.2 33.566 143 81 4 86 214 167 309 2.74e-15 75.5
Msa0670900 AT5G29000.1 33.566 143 81 4 86 214 124 266 2.77e-15 75.1
Msa0670900 AT5G29000.3 33.566 143 81 4 86 214 124 266 2.77e-15 75.1
Msa0670900 AT2G01060.1 48.193 83 40 2 126 205 4 86 3.81e-15 73.9
Msa0670900 AT5G29000.4 37.288 118 60 4 86 189 167 284 5.09e-15 73.9
Msa0670900 AT3G10760.1 50.000 72 34 1 123 194 93 162 7.03e-15 73.9
Msa0670900 AT5G05090.1 51.471 68 31 1 127 194 73 138 7.36e-15 72.8
Msa0670900 AT3G46640.3 49.296 71 36 0 136 206 143 213 7.50e-15 73.6
Msa0670900 AT3G46640.2 49.296 71 36 0 136 206 143 213 7.81e-15 73.6
Msa0670900 AT3G46640.1 49.296 71 36 0 136 206 143 213 7.81e-15 73.6
Msa0670900 AT3G04450.1 50.794 63 30 1 128 189 229 291 1.23e-14 73.6
Msa0670900 AT3G04450.4 50.794 63 30 1 128 189 189 251 1.26e-14 73.6
Msa0670900 AT3G04450.2 50.794 63 30 1 128 189 189 251 1.26e-14 73.6
Msa0670900 AT3G04450.3 50.794 63 30 1 128 189 229 291 1.49e-14 72.8
Msa0670900 AT2G40970.1 54.237 59 27 0 136 194 104 162 2.63e-14 71.2
Msa0670900 AT5G44190.1 58.621 58 24 0 136 193 146 203 5.39e-14 71.6
Msa0670900 AT5G44190.2 58.621 58 24 0 136 193 146 203 6.47e-14 71.2
Msa0670900 AT4G28610.1 60.000 55 21 1 137 190 225 279 9.83e-14 70.9
Msa0670900 AT2G20570.1 51.351 74 32 1 136 205 152 225 1.65e-13 70.1
Msa0670900 AT1G69580.1 49.231 65 32 1 137 200 34 98 3.20e-13 68.9
Msa0670900 AT1G69580.2 49.231 65 32 1 137 200 34 98 3.28e-13 68.9
Msa0670900 AT3G24120.1 38.182 110 50 4 137 239 41 139 3.61e-13 68.6
Msa0670900 AT3G24120.2 38.182 110 50 4 137 239 41 139 3.96e-13 68.6
Msa0670900 AT4G13640.2 39.091 110 49 5 137 239 37 135 5.63e-13 67.8
Msa0670900 AT4G13640.1 39.091 110 49 5 137 239 37 135 5.82e-13 67.8
Msa0670900 AT1G49190.2 34.314 102 62 2 1 99 52 151 5.85e-13 68.9
Msa0670900 AT2G20570.2 52.113 71 30 1 139 205 171 241 1.02e-12 67.8
Msa0670900 AT1G14600.1 61.538 52 19 1 139 189 25 76 1.04e-12 66.6
Msa0670900 AT2G40260.1 49.275 69 31 2 135 202 80 145 1.17e-12 67.8
Msa0670900 AT1G25550.1 48.000 75 38 1 123 196 195 269 1.60e-12 67.0
Msa0670900 AT3G13040.3 50.769 65 30 2 128 190 231 295 1.60e-12 67.4
Msa0670900 AT3G13040.1 50.769 65 30 2 128 190 231 295 1.60e-12 67.4
Msa0670900 AT3G13040.2 50.769 65 30 2 128 190 231 295 1.60e-12 67.4
Msa0670900 AT5G45580.1 37.838 111 60 3 133 241 3 106 1.67e-12 66.2
Msa0670900 AT5G45580.2 37.838 111 60 3 133 241 19 122 1.72e-12 66.2
Msa0670900 AT5G06800.3 58.182 55 22 1 137 190 192 246 2.78e-12 65.9
Msa0670900 AT5G06800.1 50.000 68 31 2 137 203 192 257 3.58e-12 66.2
Msa0670900 AT5G06800.2 58.182 55 22 1 137 190 192 246 4.03e-12 65.9
Msa0670900 AT4G04605.1 50.000 66 31 2 135 198 12 77 4.05e-12 64.7
Msa0670900 AT1G79430.2 48.000 75 34 2 137 206 34 108 6.88e-12 65.1
Msa0670900 AT2G20400.2 59.259 54 21 1 137 189 231 284 7.06e-12 65.5
Msa0670900 AT2G20400.1 59.259 54 21 1 137 189 231 284 7.38e-12 65.5
Msa0670900 AT3G12730.1 52.727 55 25 1 137 190 23 77 1.27e-11 63.5
Msa0670900 AT4G18020.8 50.877 57 28 0 137 193 296 352 2.24e-11 63.9
Msa0670900 AT4G18020.11 50.877 57 28 0 137 193 296 352 2.24e-11 63.9
Msa0670900 AT4G18020.4 50.877 57 28 0 137 193 296 352 2.24e-11 63.9
Msa0670900 AT4G18020.5 50.877 57 28 0 137 193 296 352 2.24e-11 63.9
Msa0670900 AT4G18020.10 50.877 57 28 0 137 193 296 352 2.24e-11 63.9
Msa0670900 AT4G18020.7 50.877 57 28 0 137 193 296 352 2.24e-11 63.9
Msa0670900 AT4G18020.3 50.877 57 28 0 137 193 296 352 2.29e-11 63.9
Msa0670900 AT4G18020.2 50.877 57 28 0 137 193 296 352 2.29e-11 63.9
Msa0670900 AT4G18020.1 50.877 57 28 0 137 193 296 352 2.29e-11 63.9
Msa0670900 AT4G18020.6 50.877 57 28 0 137 193 296 352 2.29e-11 63.9
Msa0670900 AT4G18020.9 50.877 57 28 0 137 193 296 352 2.29e-11 63.9
Msa0670900 AT2G03500.1 50.000 62 30 1 136 196 232 293 3.13e-11 63.5
Msa0670900 AT2G38300.1 48.214 56 28 1 135 189 52 107 3.99e-11 62.8
Msa0670900 AT2G42660.1 39.560 91 48 3 138 223 51 139 5.25e-11 62.0
Msa0670900 AT2G02060.2 55.769 52 22 1 139 189 31 82 8.57e-11 61.2
Msa0670900 AT2G02060.1 55.769 52 22 1 139 189 31 82 8.57e-11 61.2

Find 61 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGATTCTCAAAATTA+TGG 0.243357 4_4:+13754417 Msa0670900:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4_4:+13754462 Msa0670900:CDS
AACTGCATCAACGATTTCTA+TGG 0.292867 4_4:+13754270 Msa0670900:CDS
ATTATGGATGTTCCTGGTTT+AGG 0.300681 4_4:+13754433 Msa0670900:CDS
ATCCTACCTTTACAACCTTA+TGG 0.307805 4_4:+13754829 Msa0670900:CDS
CTCAAAATTATGGATGTTCC+TGG 0.317981 4_4:+13754427 Msa0670900:CDS
CTTGAATTTGTTGGAAAGAA+TGG 0.358508 4_4:+13753802 Msa0670900:CDS
AGAAATCGTTGATGCAGTTC+TGG 0.372917 4_4:-13754266 None:intergenic
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4_4:+13753793 Msa0670900:CDS
TAGCTGAGTGGATTGATTGC+GGG 0.380816 4_4:+13754801 Msa0670900:CDS
AAAGTGACTAACCATGATAA+TGG 0.381195 4_4:-13753635 None:intergenic
TACATATTGGAGTAAGCCTT+TGG 0.400446 4_4:+13754070 Msa0670900:CDS
GGTTGAAGTAAATAGAAAAC+GGG 0.409322 4_4:+13754181 Msa0670900:CDS
ATCAGAAGCTGTGTTACATT+TGG 0.412873 4_4:-13753777 None:intergenic
AATAAAGAATATGAAATTGT+TGG 0.416766 4_4:+13754149 Msa0670900:CDS
GAGTGGATTGATTGCGGGGC+GGG 0.418434 4_4:+13754806 Msa0670900:CDS
CATTCTTTCCAACAAATTCA+AGG 0.419108 4_4:-13753801 None:intergenic
AAACTGCATATTAGGCCATA+AGG 0.423301 4_4:-13754844 None:intergenic
AATGTGGGGCTTGTACATAT+TGG 0.427702 4_4:+13754057 Msa0670900:CDS
AATGTCGTTTGCAGAAATAT+AGG 0.441994 4_4:+13754591 Msa0670900:intron
TGTAATCGATTCCATTATCA+TGG 0.443909 4_4:+13753624 Msa0670900:CDS
CAGTTTGAAAGTTATCGTTC+CGG 0.444561 4_4:-13754745 None:intergenic
TCAAACTGAGCTGACTGATC+AGG 0.459166 4_4:+13754760 Msa0670900:CDS
TTCTGGCGACCAAGACAAAC+GGG 0.460294 4_4:-13754249 None:intergenic
AATAAATTCTTACTAATGAC+AGG 0.461511 4_4:-13753914 None:intergenic
ATATGAAATTGTTGGAAGCT+TGG 0.461542 4_4:+13754157 Msa0670900:CDS
AAAAGATACACATGCAAAGA+AGG 0.470493 4_4:+13754226 Msa0670900:CDS
AAGTCATAAGCATCCATGTT+TGG 0.471983 4_4:-13753860 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_4:+13754240 Msa0670900:CDS
TGGTTGAAGTAAATAGAAAA+CGG 0.479913 4_4:+13754180 Msa0670900:CDS
CAGCTAGAAAACCATCATTC+CGG 0.492887 4_4:-13754784 None:intergenic
GTTCTGGCGACCAAGACAAA+CGG 0.493157 4_4:-13754250 None:intergenic
CTAGCTGAGTGGATTGATTG+CGG 0.505708 4_4:+13754800 Msa0670900:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4_4:-13754310 None:intergenic
GATGTTCCTGGTTTAGGTGT+AGG 0.515547 4_4:+13754439 Msa0670900:CDS
CTATGGGCTGTCAATCAACT+AGG 0.518108 4_4:+13754287 Msa0670900:CDS
ACTGATCAGGACCGGAATGA+TGG 0.523712 4_4:+13754773 Msa0670900:CDS
TTATGAAGTCTATTCAATGT+GGG 0.525603 4_4:+13754042 Msa0670900:CDS
TCAAACTCAATCGATCGATC+AGG 0.527821 4_4:+13754721 Msa0670900:CDS
ATTAGGCCATAAGGTTGTAA+AGG 0.531488 4_4:-13754835 None:intergenic
TTGACGTCTTCTTCAACCAA+AGG 0.537631 4_4:-13754086 None:intergenic
TGGCAACATGTTGTCAGAAA+CGG 0.543020 4_4:+13754116 Msa0670900:CDS
ATTATGAAGTCTATTCAATG+TGG 0.558796 4_4:+13754041 Msa0670900:CDS
ATTGACAAGGTTCATAGTGA+TGG 0.561362 4_4:+13754695 Msa0670900:CDS
AGCTGAGTGGATTGATTGCG+GGG 0.562343 4_4:+13754802 Msa0670900:CDS
CTCAATCGATCGATCAGGAC+CGG 0.563858 4_4:+13754726 Msa0670900:CDS
CTGAGCTGACTGATCAGGAC+CGG 0.566450 4_4:+13754765 Msa0670900:CDS
TGAAATTGTTGGAAGCTTGG+TGG 0.576315 4_4:+13754160 Msa0670900:CDS
ACTGCATCAACGATTTCTAT+GGG 0.587946 4_4:+13754271 Msa0670900:CDS
TCAAGACGAGTGCATTGACA+AGG 0.590781 4_4:+13754682 Msa0670900:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4_4:-13754831 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 4_4:+13753847 Msa0670900:CDS
TATGAAGTCTATTCAATGTG+GGG 0.614025 4_4:+13754043 Msa0670900:CDS
AAGAAGACGTCAAGAGCATG+TGG 0.649026 4_4:+13754096 Msa0670900:CDS
CTGATGAAACAAAACGTGGG+AGG 0.661820 4_4:+13754633 Msa0670900:CDS
ACATGTCCTACACCTAAACC+AGG 0.664952 4_4:-13754445 None:intergenic
TGAGTGGATTGATTGCGGGG+CGG 0.687161 4_4:+13754805 Msa0670900:CDS
GTGGGAGGAAAAGATCAAAG+TGG 0.698947 4_4:+13754648 Msa0670900:CDS
CAGCTGATGAAACAAAACGT+GGG 0.702126 4_4:+13754630 Msa0670900:CDS
TCAGCTGATGAAACAAAACG+TGG 0.708886 4_4:+13754629 Msa0670900:CDS
GTTGAAGTAAATAGAAAACG+GGG 0.738525 4_4:+13754182 Msa0670900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4_4:13754149-13754168 Msa0670900:CDS 15.0%
!! AAAAATAAGTGAGATAAAAA+TGG - chr4_4:13754384-13754403 None:intergenic 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4_4:13754418-13754437 None:intergenic 15.0%
!! AATAAAGAATATGAAATTGT+TGG + chr4_4:13754149-13754168 Msa0670900:CDS 15.0%
!! AAAAATAAGTGAGATAAAAA+TGG - chr4_4:13754384-13754403 None:intergenic 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4_4:13754418-13754437 None:intergenic 15.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13753917-13753936 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG + chr4_4:13753995-13754014 Msa0670900:intron 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4_4:13754419-13754438 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4_4:13754417-13754436 Msa0670900:CDS 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13753917-13753936 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG + chr4_4:13753995-13754014 Msa0670900:intron 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4_4:13754419-13754438 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4_4:13754417-13754436 Msa0670900:CDS 20.0%
! ATTATGAAGTCTATTCAATG+TGG + chr4_4:13754041-13754060 Msa0670900:CDS 25.0%
! TTATGAAGTCTATTCAATGT+GGG + chr4_4:13754042-13754061 Msa0670900:CDS 25.0%
! TGGTTGAAGTAAATAGAAAA+CGG + chr4_4:13754180-13754199 Msa0670900:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4_4:13754333-13754352 None:intergenic 25.0%
! ATTATGAAGTCTATTCAATG+TGG + chr4_4:13754041-13754060 Msa0670900:CDS 25.0%
! TTATGAAGTCTATTCAATGT+GGG + chr4_4:13754042-13754061 Msa0670900:CDS 25.0%
! TGGTTGAAGTAAATAGAAAA+CGG + chr4_4:13754180-13754199 Msa0670900:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4_4:13754333-13754352 None:intergenic 25.0%
! TGTAATCGATTCCATTATCA+TGG + chr4_4:13753624-13753643 Msa0670900:CDS 30.0%
AAAGTGACTAACCATGATAA+TGG - chr4_4:13753638-13753657 None:intergenic 30.0%
CATTCTTTCCAACAAATTCA+AGG - chr4_4:13753804-13753823 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+TGG + chr4_4:13753802-13753821 Msa0670900:CDS 30.0%
!!! TGAGTTTTAGGGTAACATTT+TGG - chr4_4:13753889-13753908 None:intergenic 30.0%
TATGAAGTCTATTCAATGTG+GGG + chr4_4:13754043-13754062 Msa0670900:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4_4:13754157-13754176 Msa0670900:CDS 30.0%
GGTTGAAGTAAATAGAAAAC+GGG + chr4_4:13754181-13754200 Msa0670900:CDS 30.0%
GTTGAAGTAAATAGAAAACG+GGG + chr4_4:13754182-13754201 Msa0670900:CDS 30.0%
! TTTGCATGTGTATCTTTTGA+GGG - chr4_4:13754224-13754243 None:intergenic 30.0%
AAAAGATACACATGCAAAGA+AGG + chr4_4:13754226-13754245 Msa0670900:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_4:13754313-13754332 None:intergenic 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4_4:13754473-13754492 Msa0670900:intron 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4_4:13754591-13754610 Msa0670900:intron 30.0%
! TGTAATCGATTCCATTATCA+TGG + chr4_4:13753624-13753643 Msa0670900:CDS 30.0%
AAAGTGACTAACCATGATAA+TGG - chr4_4:13753638-13753657 None:intergenic 30.0%
CATTCTTTCCAACAAATTCA+AGG - chr4_4:13753804-13753823 None:intergenic 30.0%
!! CTTGAATTTGTTGGAAAGAA+TGG + chr4_4:13753802-13753821 Msa0670900:CDS 30.0%
!!! TGAGTTTTAGGGTAACATTT+TGG - chr4_4:13753889-13753908 None:intergenic 30.0%
TATGAAGTCTATTCAATGTG+GGG + chr4_4:13754043-13754062 Msa0670900:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4_4:13754157-13754176 Msa0670900:CDS 30.0%
GGTTGAAGTAAATAGAAAAC+GGG + chr4_4:13754181-13754200 Msa0670900:CDS 30.0%
GTTGAAGTAAATAGAAAACG+GGG + chr4_4:13754182-13754201 Msa0670900:CDS 30.0%
! TTTGCATGTGTATCTTTTGA+GGG - chr4_4:13754224-13754243 None:intergenic 30.0%
AAAAGATACACATGCAAAGA+AGG + chr4_4:13754226-13754245 Msa0670900:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_4:13754313-13754332 None:intergenic 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4_4:13754473-13754492 Msa0670900:intron 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4_4:13754591-13754610 Msa0670900:intron 30.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4_4:13753780-13753799 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_4:13753793-13753812 Msa0670900:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4_4:13753863-13753882 None:intergenic 35.0%
! GACAGGAATGTTGAGTTTTA+GGG - chr4_4:13753900-13753919 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+AGG - chr4_4:13753901-13753920 None:intergenic 35.0%
TACATATTGGAGTAAGCCTT+TGG + chr4_4:13754070-13754089 Msa0670900:CDS 35.0%
! TTGCATGTGTATCTTTTGAG+GGG - chr4_4:13754223-13754242 None:intergenic 35.0%
! CTTTGCATGTGTATCTTTTG+AGG - chr4_4:13754225-13754244 None:intergenic 35.0%
AACTGCATCAACGATTTCTA+TGG + chr4_4:13754270-13754289 Msa0670900:CDS 35.0%
ACTGCATCAACGATTTCTAT+GGG + chr4_4:13754271-13754290 Msa0670900:CDS 35.0%
CTCAAAATTATGGATGTTCC+TGG + chr4_4:13754427-13754446 Msa0670900:CDS 35.0%
ATTATGGATGTTCCTGGTTT+AGG + chr4_4:13754433-13754452 Msa0670900:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4_4:13754462-13754481 Msa0670900:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4_4:13754477-13754496 Msa0670900:intron 35.0%
ATTGACAAGGTTCATAGTGA+TGG + chr4_4:13754695-13754714 Msa0670900:CDS 35.0%
CAGTTTGAAAGTTATCGTTC+CGG - chr4_4:13754748-13754767 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4_4:13754829-13754848 Msa0670900:CDS 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4_4:13753780-13753799 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4_4:13753793-13753812 Msa0670900:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4_4:13753863-13753882 None:intergenic 35.0%
! GACAGGAATGTTGAGTTTTA+GGG - chr4_4:13753900-13753919 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+AGG - chr4_4:13753901-13753920 None:intergenic 35.0%
TACATATTGGAGTAAGCCTT+TGG + chr4_4:13754070-13754089 Msa0670900:CDS 35.0%
! TTGCATGTGTATCTTTTGAG+GGG - chr4_4:13754223-13754242 None:intergenic 35.0%
! CTTTGCATGTGTATCTTTTG+AGG - chr4_4:13754225-13754244 None:intergenic 35.0%
AACTGCATCAACGATTTCTA+TGG + chr4_4:13754270-13754289 Msa0670900:CDS 35.0%
ACTGCATCAACGATTTCTAT+GGG + chr4_4:13754271-13754290 Msa0670900:CDS 35.0%
CTCAAAATTATGGATGTTCC+TGG + chr4_4:13754427-13754446 Msa0670900:CDS 35.0%
ATTATGGATGTTCCTGGTTT+AGG + chr4_4:13754433-13754452 Msa0670900:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4_4:13754462-13754481 Msa0670900:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4_4:13754477-13754496 Msa0670900:intron 35.0%
ATTGACAAGGTTCATAGTGA+TGG + chr4_4:13754695-13754714 Msa0670900:CDS 35.0%
CAGTTTGAAAGTTATCGTTC+CGG - chr4_4:13754748-13754767 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4_4:13754829-13754848 Msa0670900:CDS 35.0%
TGATGCTCATATGCCAAACA+TGG + chr4_4:13753847-13753866 Msa0670900:CDS 40.0%
AATGTGGGGCTTGTACATAT+TGG + chr4_4:13754057-13754076 Msa0670900:CDS 40.0%
TTGACGTCTTCTTCAACCAA+AGG - chr4_4:13754089-13754108 None:intergenic 40.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4_4:13754116-13754135 Msa0670900:CDS 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4_4:13754160-13754179 Msa0670900:CDS 40.0%
AGAAATCGTTGATGCAGTTC+TGG - chr4_4:13754269-13754288 None:intergenic 40.0%
TCAGCTGATGAAACAAAACG+TGG + chr4_4:13754629-13754648 Msa0670900:CDS 40.0%
CAGCTGATGAAACAAAACGT+GGG + chr4_4:13754630-13754649 Msa0670900:CDS 40.0%
TCAAACTCAATCGATCGATC+AGG + chr4_4:13754721-13754740 Msa0670900:CDS 40.0%
CAGCTAGAAAACCATCATTC+CGG - chr4_4:13754787-13754806 None:intergenic 40.0%
!!! ATGATGGTTTTCTAGCTGAG+TGG + chr4_4:13754789-13754808 Msa0670900:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4_4:13753847-13753866 Msa0670900:CDS 40.0%
AATGTGGGGCTTGTACATAT+TGG + chr4_4:13754057-13754076 Msa0670900:CDS 40.0%
TTGACGTCTTCTTCAACCAA+AGG - chr4_4:13754089-13754108 None:intergenic 40.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4_4:13754116-13754135 Msa0670900:CDS 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4_4:13754160-13754179 Msa0670900:CDS 40.0%
AGAAATCGTTGATGCAGTTC+TGG - chr4_4:13754269-13754288 None:intergenic 40.0%
TCAGCTGATGAAACAAAACG+TGG + chr4_4:13754629-13754648 Msa0670900:CDS 40.0%
CAGCTGATGAAACAAAACGT+GGG + chr4_4:13754630-13754649 Msa0670900:CDS 40.0%
TCAAACTCAATCGATCGATC+AGG + chr4_4:13754721-13754740 Msa0670900:CDS 40.0%
CAGCTAGAAAACCATCATTC+CGG - chr4_4:13754787-13754806 None:intergenic 40.0%
!!! ATGATGGTTTTCTAGCTGAG+TGG + chr4_4:13754789-13754808 Msa0670900:CDS 40.0%
! AAGAAGACGTCAAGAGCATG+TGG + chr4_4:13754096-13754115 Msa0670900:CDS 45.0%
! CTATGGGCTGTCAATCAACT+AGG + chr4_4:13754287-13754306 Msa0670900:CDS 45.0%
GATGTTCCTGGTTTAGGTGT+AGG + chr4_4:13754439-13754458 Msa0670900:CDS 45.0%
ACATGTCCTACACCTAAACC+AGG - chr4_4:13754448-13754467 None:intergenic 45.0%
CTGATGAAACAAAACGTGGG+AGG + chr4_4:13754633-13754652 Msa0670900:CDS 45.0%
GTGGGAGGAAAAGATCAAAG+TGG + chr4_4:13754648-13754667 Msa0670900:CDS 45.0%
! TCAAGACGAGTGCATTGACA+AGG + chr4_4:13754682-13754701 Msa0670900:CDS 45.0%
TCAAACTGAGCTGACTGATC+AGG + chr4_4:13754760-13754779 Msa0670900:CDS 45.0%
!! CTAGCTGAGTGGATTGATTG+CGG + chr4_4:13754800-13754819 Msa0670900:CDS 45.0%
!! TAGCTGAGTGGATTGATTGC+GGG + chr4_4:13754801-13754820 Msa0670900:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4_4:13754834-13754853 None:intergenic 45.0%
! AAGAAGACGTCAAGAGCATG+TGG + chr4_4:13754096-13754115 Msa0670900:CDS 45.0%
! CTATGGGCTGTCAATCAACT+AGG + chr4_4:13754287-13754306 Msa0670900:CDS 45.0%
GATGTTCCTGGTTTAGGTGT+AGG + chr4_4:13754439-13754458 Msa0670900:CDS 45.0%
ACATGTCCTACACCTAAACC+AGG - chr4_4:13754448-13754467 None:intergenic 45.0%
CTGATGAAACAAAACGTGGG+AGG + chr4_4:13754633-13754652 Msa0670900:CDS 45.0%
GTGGGAGGAAAAGATCAAAG+TGG + chr4_4:13754648-13754667 Msa0670900:CDS 45.0%
! TCAAGACGAGTGCATTGACA+AGG + chr4_4:13754682-13754701 Msa0670900:CDS 45.0%
TCAAACTGAGCTGACTGATC+AGG + chr4_4:13754760-13754779 Msa0670900:CDS 45.0%
!! CTAGCTGAGTGGATTGATTG+CGG + chr4_4:13754800-13754819 Msa0670900:CDS 45.0%
!! TAGCTGAGTGGATTGATTGC+GGG + chr4_4:13754801-13754820 Msa0670900:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4_4:13754834-13754853 None:intergenic 45.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13754240-13754259 Msa0670900:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13754252-13754271 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_4:13754253-13754272 None:intergenic 50.0%
CTCAATCGATCGATCAGGAC+CGG + chr4_4:13754726-13754745 Msa0670900:CDS 50.0%
ACTGATCAGGACCGGAATGA+TGG + chr4_4:13754773-13754792 Msa0670900:CDS 50.0%
!! AGCTGAGTGGATTGATTGCG+GGG + chr4_4:13754802-13754821 Msa0670900:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13754240-13754259 Msa0670900:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13754252-13754271 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_4:13754253-13754272 None:intergenic 50.0%
CTCAATCGATCGATCAGGAC+CGG + chr4_4:13754726-13754745 Msa0670900:CDS 50.0%
ACTGATCAGGACCGGAATGA+TGG + chr4_4:13754773-13754792 Msa0670900:CDS 50.0%
!! AGCTGAGTGGATTGATTGCG+GGG + chr4_4:13754802-13754821 Msa0670900:CDS 50.0%
CTGAGCTGACTGATCAGGAC+CGG + chr4_4:13754765-13754784 Msa0670900:CDS 55.0%
!! TGAGTGGATTGATTGCGGGG+CGG + chr4_4:13754805-13754824 Msa0670900:CDS 55.0%
CTGAGCTGACTGATCAGGAC+CGG + chr4_4:13754765-13754784 Msa0670900:CDS 55.0%
!! TGAGTGGATTGATTGCGGGG+CGG + chr4_4:13754805-13754824 Msa0670900:CDS 55.0%
!! GAGTGGATTGATTGCGGGGC+GGG + chr4_4:13754806-13754825 Msa0670900:CDS 60.0%
!! GAGTGGATTGATTGCGGGGC+GGG + chr4_4:13754806-13754825 Msa0670900:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 13753621 13754856 13753621 ID=Msa0670900;Name=Msa0670900
chr4_4 mRNA 13753621 13754856 13753621 ID=Msa0670900-mRNA-1;Parent=Msa0670900;Name=Msa0670900-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0|0|1|1|1|5|0|269
chr4_4 exon 13753621 13753645 13753621 ID=Msa0670900-mRNA-1:exon:4187;Parent=Msa0670900-mRNA-1
chr4_4 exon 13753774 13753923 13753774 ID=Msa0670900-mRNA-1:exon:4188;Parent=Msa0670900-mRNA-1
chr4_4 exon 13754012 13754317 13754012 ID=Msa0670900-mRNA-1:exon:4189;Parent=Msa0670900-mRNA-1
chr4_4 exon 13754407 13754483 13754407 ID=Msa0670900-mRNA-1:exon:4190;Parent=Msa0670900-mRNA-1
chr4_4 exon 13754605 13754856 13754605 ID=Msa0670900-mRNA-1:exon:4191;Parent=Msa0670900-mRNA-1
chr4_4 CDS 13753621 13753645 13753621 ID=Msa0670900-mRNA-1:cds;Parent=Msa0670900-mRNA-1
chr4_4 CDS 13753774 13753923 13753774 ID=Msa0670900-mRNA-1:cds;Parent=Msa0670900-mRNA-1
chr4_4 CDS 13754012 13754317 13754012 ID=Msa0670900-mRNA-1:cds;Parent=Msa0670900-mRNA-1
chr4_4 CDS 13754407 13754483 13754407 ID=Msa0670900-mRNA-1:cds;Parent=Msa0670900-mRNA-1
chr4_4 CDS 13754605 13754856 13754605 ID=Msa0670900-mRNA-1:cds;Parent=Msa0670900-mRNA-1
Gene Sequence

>Msa0670900

ATGTGTAATCGATTCCATTATCATGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAATGGACTGTTTCGATTTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTAAAACTCAACATTCCTGTCATTATGATGGCTGTTGATTTCGCAAAAAGTTCTATTATGAAGTCTATTCAATGTGGGGCTTGTACATATTGGAGTAAGCCTTTGGTTGAAGAAGACGTCAAGAGCATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTGAAGTAAATAGAAAACGGGGTAGAGAAGATGATAACCCCTCAAAAGATACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAACTGCATCAACGATTTCTATGGGCTGTCAATCAACTAGGCCTTGATAAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCTGGTTTAGGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGGATTCATTTGAAGAGATCAGCTGATGAAACAAAACGTGGGAGGAAAAGATCAAAGTGGAGTGATAGCTTTCAAGACGAGTGCATTGACAAGGTTCATAGTGATGGCTTTCAAACTCAATCGATCGATCAGGACCGGAACGATAACTTTCAAACTGAGCTGACTGATCAGGACCGGAATGATGGTTTTCTAGCTGAGTGGATTGATTGCGGGGCGGGATCCTACCTTTACAACCTTATGGCCTAA
Protein sequence

>Msa0670900

MCNRFHYHVTKCNTASDALNLLERMDCFDLMLIDAHMPNMDAYDFVQNVTLKLNIPVIMMAVDFAKSSIMKSIQCGACTYWSKPLVEEDVKSMWQHVVRNGSTENKEYEIVGSLVVEVNRKRGREDDNPSKDTHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKRGRKRSKWSDSFQDECIDKVHSDGFQTQSIDQDRNDNFQTELTDQDRNDGFLAEWIDCGAGSYLYNLMA*