AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670910 MtrunA17_Chr4g0009851 83.121 314 50 2 1 313 1 312 0.0 541
Msa0670910 MtrunA17_Chr4g0009981 85.441 261 36 1 53 313 1 259 4.28e-167 464
Msa0670910 MtrunA17_Chr4g0009901 76.033 242 56 1 1 242 1 240 1.50e-129 370
Msa0670910 MtrunA17_Chr4g0011071 78.417 139 28 1 55 193 35 171 1.05e-75 229
Msa0670910 MtrunA17_Chr1g0161431 45.968 248 101 5 10 226 11 256 7.03e-67 220
Msa0670910 MtrunA17_Chr6g0471041 43.934 305 128 9 10 296 14 293 4.72e-66 213
Msa0670910 MtrunA17_Chr3g0123131 40.984 244 114 4 10 225 13 254 5.38e-58 196
Msa0670910 MtrunA17_Chr8g0376881 41.463 246 116 4 6 225 21 264 9.76e-58 194
Msa0670910 MtrunA17_Chr4g0009991 78.992 119 22 2 101 219 1 116 9.98e-58 182
Msa0670910 MtrunA17_Chr2g0294911 39.474 266 133 4 10 249 29 292 1.00e-55 190
Msa0670910 MtrunA17_Chr4g0067981 40.741 243 116 3 10 226 31 271 1.13e-55 191
Msa0670910 MtrunA17_Chr1g0149841 39.535 258 128 5 7 236 2 259 7.24e-52 177
Msa0670910 MtrunA17_Chr8g0391911 39.256 242 119 4 4 219 3 242 1.26e-49 166
Msa0670910 MtrunA17_Chr4g0010001 82.022 89 16 0 1 89 1 89 1.68e-49 159
Msa0670910 MtrunA17_Chr3g0137101 38.462 247 122 3 10 226 8 254 2.16e-49 174
Msa0670910 MtrunA17_Chr7g0275401 36.364 253 134 4 7 232 2 254 1.68e-47 161
Msa0670910 MtrunA17_Chr5g0423031 80.899 89 17 0 1 89 1 89 5.93e-47 156
Msa0670910 MtrunA17_Chr2g0304841 36.078 255 128 4 10 229 18 272 4.14e-46 165
Msa0670910 MtrunA17_Chr7g0275891 34.615 260 131 5 7 227 2 261 8.01e-46 158
Msa0670910 MtrunA17_Chr4g0074401 33.466 251 139 4 10 233 17 266 1.13e-43 157
Msa0670910 MtrunA17_Chr8g0383961 40.433 277 123 11 1 265 1 247 1.78e-43 150
Msa0670910 MtrunA17_Chr4g0074411 34.137 249 139 4 9 233 17 264 3.76e-43 156
Msa0670910 MtrunA17_Chr1g0149851 35.632 261 127 4 7 226 2 262 1.13e-42 154
Msa0670910 MtrunA17_Chr6g0456631 43.750 160 86 2 4 161 3 160 6.33e-41 140
Msa0670910 MtrunA17_Chr3g0134461 38.208 212 102 4 10 219 8 192 1.82e-40 140
Msa0670910 MtrunA17_Chr3g0134481 36.150 213 98 3 10 221 8 183 3.24e-38 134
Msa0670910 MtrunA17_Chr4g0074421 36.813 182 93 3 59 219 2 182 3.59e-36 129
Msa0670910 MtrunA17_Chr1g0149831 38.776 196 90 5 68 236 1 193 6.66e-33 121
Msa0670910 MtrunA17_Chr6g0473731 32.222 180 110 3 89 259 10 186 4.87e-22 95.9
Msa0670910 MtrunA17_Chr8g0350761 24.518 363 212 14 6 309 15 374 7.26e-21 92.8
Msa0670910 MtrunA17_Chr7g0223971 30.348 201 96 5 91 249 1 199 3.06e-19 88.2
Msa0670910 MtrunA17_Chr5g0417521 59.259 54 21 1 170 222 15 68 1.47e-15 76.3
Msa0670910 MtrunA17_Chr2g0324891 41.758 91 51 2 138 226 127 217 2.33e-15 76.3
Msa0670910 MtrunA17_Chr6g0461641 59.259 54 21 1 170 222 42 95 7.63e-15 74.7
Msa0670910 MtrunA17_Chr4g0040481 50.000 58 29 0 169 226 157 214 1.21e-14 73.9
Msa0670910 MtrunA17_Chr7g0255711 55.172 58 26 0 169 226 90 147 1.32e-14 73.2
Msa0670910 MtrunA17_Chr4g0031961 53.448 58 27 0 169 226 130 187 1.80e-14 72.8
Msa0670910 MtrunA17_Chr2g0321461 57.407 54 22 1 170 222 45 98 2.94e-14 72.8
Msa0670910 MtrunA17_Chr7g0261911 47.674 86 42 3 161 245 253 336 5.47e-14 72.4
Msa0670910 MtrunA17_Chr7g0254981 42.000 100 51 3 161 257 41 136 7.01e-14 70.5
Msa0670910 MtrunA17_Chr4g0054981 37.349 83 52 0 169 251 309 391 5.53e-13 69.3
Msa0670910 MtrunA17_Chr5g0400241 37.349 83 52 0 169 251 310 392 7.05e-13 68.9
Msa0670910 MtrunA17_Chr4g0042451 53.704 54 24 1 170 222 17 70 7.94e-13 68.2
Msa0670910 MtrunA17_Chr7g0274471 48.571 70 35 1 170 238 218 287 1.34e-12 67.8
Msa0670910 MtrunA17_Chr1g0190301 53.448 58 26 1 166 222 171 228 3.13e-12 66.6
Msa0670910 MtrunA17_Chr2g0291021 59.259 54 21 1 170 222 49 102 5.25e-12 65.9
Msa0670910 MtrunA17_Chr1g0198601 39.362 94 49 2 152 244 200 286 7.66e-12 65.5
Msa0670910 MtrunA17_Chr1g0162931 46.377 69 35 2 156 223 5 72 2.06e-11 63.5
Msa0670910 MtrunA17_Chr8g0356501 42.697 89 49 2 170 256 37 125 2.11e-11 63.9
Msa0670910 MtrunA17_Chr7g0242521 54.286 70 23 4 157 222 5 69 2.81e-11 62.4
Msa0670910 MtrunA17_Chr7g0243081 51.786 56 26 1 168 222 69 124 2.90e-11 63.5
Msa0670910 MtrunA17_Chr3g0095611 41.892 74 38 2 168 236 17 90 3.37e-11 62.0
Msa0670910 MtrunA17_Chr6g0479431 47.541 61 31 1 163 222 195 255 6.42e-11 62.8
Msa0670910 MtrunA17_Chr1g0175091 44.118 68 35 2 168 232 256 323 7.21e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670910 AT2G01760.1 39.695 262 117 6 7 231 4 261 3.57e-58 192
Msa0670910 AT1G67710.1 39.623 265 128 6 11 243 8 272 1.94e-54 185
Msa0670910 AT2G01760.2 35.862 290 117 7 7 231 4 289 2.81e-52 177
Msa0670910 AT3G16857.1 38.697 261 116 6 10 226 33 293 7.94e-51 178
Msa0670910 AT3G16857.2 37.165 261 120 4 10 226 33 293 1.11e-50 178
Msa0670910 AT4G31920.1 35.743 249 147 3 10 248 13 258 8.90e-48 168
Msa0670910 AT2G25180.1 38.272 243 120 4 10 226 13 251 2.02e-46 165
Msa0670910 AT5G58080.1 39.200 250 126 6 10 236 14 260 1.21e-41 152
Msa0670910 AT3G16857.3 37.838 222 94 6 49 226 2 223 1.13e-39 147
Msa0670910 AT2G01760.3 37.500 200 85 5 68 231 1 196 9.28e-38 137
Msa0670910 AT1G67710.2 39.614 207 94 5 68 243 1 207 1.96e-36 136
Msa0670910 AT5G49240.1 31.780 236 136 5 9 219 37 272 1.77e-34 127
Msa0670910 AT1G49190.1 29.752 242 141 5 10 222 30 271 1.37e-30 119
Msa0670910 AT3G62670.1 31.944 216 126 5 28 222 53 268 6.29e-30 118
Msa0670910 AT1G67710.3 37.968 187 85 5 88 243 12 198 2.71e-27 111
Msa0670910 AT5G07210.2 27.407 270 142 7 14 231 16 283 6.13e-26 108
Msa0670910 AT5G07210.1 26.619 278 142 8 14 231 16 291 2.20e-23 100
Msa0670910 AT2G27070.1 24.627 268 145 7 14 226 16 281 1.31e-19 89.7
Msa0670910 AT1G49190.2 33.065 124 82 1 10 132 30 153 3.45e-18 85.5
Msa0670910 AT5G29000.4 35.862 145 75 6 103 238 166 301 1.23e-15 76.6
Msa0670910 AT5G59570.2 49.296 71 33 1 156 226 130 197 1.64e-15 75.9
Msa0670910 AT5G59570.1 49.296 71 33 1 156 226 130 197 1.64e-15 75.9
Msa0670910 AT5G29000.1 33.728 169 88 7 103 256 123 282 1.80e-15 76.6
Msa0670910 AT5G29000.3 33.728 169 88 7 103 256 123 282 1.80e-15 76.6
Msa0670910 AT5G29000.2 34.868 152 81 6 103 245 166 308 1.90e-15 76.6
Msa0670910 AT5G05090.1 44.944 89 37 2 138 226 61 137 4.07e-15 74.3
Msa0670910 AT2G01060.1 49.398 83 39 2 159 238 4 86 6.52e-15 73.9
Msa0670910 AT2G40970.1 53.448 58 27 0 169 226 104 161 7.93e-15 73.2
Msa0670910 AT3G04030.1 59.259 54 21 1 170 222 45 98 8.57e-15 74.7
Msa0670910 AT3G04030.3 59.259 54 21 1 170 222 45 98 9.48e-15 74.7
Msa0670910 AT5G44190.1 53.448 58 27 0 169 226 146 203 9.86e-15 74.7
Msa0670910 AT3G10760.1 55.172 58 26 0 169 226 104 161 1.01e-14 74.3
Msa0670910 AT5G44190.2 53.448 58 27 0 169 226 146 203 1.19e-14 73.9
Msa0670910 AT5G18240.2 52.113 71 32 2 153 222 29 98 1.20e-14 74.3
Msa0670910 AT5G18240.3 52.113 71 32 2 153 222 29 98 1.20e-14 74.3
Msa0670910 AT5G18240.5 52.113 71 32 2 153 222 29 98 1.33e-14 74.3
Msa0670910 AT5G18240.1 52.113 71 32 2 153 222 29 98 1.34e-14 74.3
Msa0670910 AT5G18240.4 52.113 71 32 2 153 222 29 98 1.34e-14 74.3
Msa0670910 AT3G46640.3 53.448 58 27 0 169 226 143 200 2.00e-14 73.2
Msa0670910 AT3G46640.2 53.448 58 27 0 169 226 143 200 2.06e-14 73.2
Msa0670910 AT3G46640.1 53.448 58 27 0 169 226 143 200 2.06e-14 73.2
Msa0670910 AT3G04030.2 56.604 53 23 0 170 222 45 97 5.40e-14 72.4
Msa0670910 AT4G28610.1 49.351 77 37 2 170 245 225 300 7.02e-14 72.0
Msa0670910 AT2G20570.1 42.683 82 41 1 151 226 128 209 7.96e-14 72.0
Msa0670910 AT3G04450.3 51.667 60 28 1 170 228 238 297 4.01e-13 69.3
Msa0670910 AT4G18020.8 39.474 76 43 1 152 227 281 353 5.88e-13 69.7
Msa0670910 AT4G18020.11 39.474 76 43 1 152 227 281 353 5.88e-13 69.7
Msa0670910 AT4G18020.4 39.474 76 43 1 152 227 281 353 5.88e-13 69.7
Msa0670910 AT4G18020.5 39.474 76 43 1 152 227 281 353 5.88e-13 69.7
Msa0670910 AT4G18020.10 39.474 76 43 1 152 227 281 353 5.88e-13 69.7
Msa0670910 AT4G18020.7 39.474 76 43 1 152 227 281 353 5.88e-13 69.7
Msa0670910 AT4G18020.3 39.474 76 43 1 152 227 281 353 6.27e-13 69.7
Msa0670910 AT4G18020.2 39.474 76 43 1 152 227 281 353 6.27e-13 69.7
Msa0670910 AT4G18020.1 39.474 76 43 1 152 227 281 353 6.27e-13 69.7
Msa0670910 AT4G18020.6 39.474 76 43 1 152 227 281 353 6.27e-13 69.7
Msa0670910 AT4G18020.9 39.474 76 43 1 152 227 281 353 6.27e-13 69.7
Msa0670910 AT3G04450.1 51.667 60 28 1 170 228 238 297 6.40e-13 69.3
Msa0670910 AT2G20570.2 52.727 55 26 0 172 226 171 225 6.49e-13 69.3
Msa0670910 AT3G04450.4 51.667 60 28 1 170 228 198 257 7.00e-13 68.9
Msa0670910 AT3G04450.2 51.667 60 28 1 170 228 198 257 7.00e-13 68.9
Msa0670910 AT1G25550.1 44.737 76 40 2 156 229 194 269 2.73e-12 67.0
Msa0670910 AT1G69580.1 51.852 54 25 1 170 222 34 87 9.02e-12 65.5
Msa0670910 AT1G69580.2 51.852 54 25 1 170 222 34 87 9.77e-12 65.5
Msa0670910 AT2G20400.2 53.125 64 27 2 170 230 231 294 1.61e-11 65.1
Msa0670910 AT2G20400.1 53.125 64 27 2 170 230 231 294 1.68e-11 65.1
Msa0670910 AT1G14600.1 56.604 53 22 1 172 223 25 77 2.70e-11 63.2
Msa0670910 AT1G79430.2 42.222 90 48 3 170 256 34 122 5.45e-11 63.2
Msa0670910 AT3G13040.3 47.059 68 33 2 169 233 240 307 7.68e-11 63.2
Msa0670910 AT3G13040.1 47.059 68 33 2 169 233 240 307 7.68e-11 63.2
Msa0670910 AT3G13040.2 47.059 68 33 2 169 233 240 307 7.68e-11 63.2
Msa0670910 AT2G40260.1 40.594 101 55 3 136 234 51 148 8.53e-11 62.8

Find 59 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGTTGCCAGCCATTTAC+AGG 0.283862 4_4:+13760610 Msa0670910:CDS
TTCAGTTACCAAATGTAGTA+TGG 0.293347 4_4:+13759879 Msa0670910:intron
TGCTAAAACGAACCTGTAAA+TGG 0.306750 4_4:-13760622 None:intergenic
GACATTGTGTGGTCGAATTC+AGG 0.320772 4_4:-13761130 None:intergenic
TGAGTTGTAGGGTAACATTT+TGG 0.376494 4_4:-13759996 None:intergenic
CACATATTGGACTAAGCCTT+TGG 0.377509 4_4:+13760209 Msa0670910:CDS
GATAAATCATCATCAACATA+TGG 0.380393 4_4:-13761242 None:intergenic
AGAAACCGCTGATGAAGTTC+TGG 0.386349 4_4:-13760411 None:intergenic
TGACAGGAATGTTGAGTTGT+AGG 0.408636 4_4:-13760008 None:intergenic
ATCAGAAGCCATACTACATT+TGG 0.409595 4_4:-13759887 None:intergenic
AACAAAGAATATGAAATTGT+TGG 0.415487 4_4:+13760294 Msa0670910:CDS
GGTTCAAACAAATAGAAAAC+GGG 0.416232 4_4:+13760326 Msa0670910:CDS
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4_4:-13759911 None:intergenic
ACGCTAAATTGTGTTTCAAA+AGG 0.431875 4_4:-13761065 None:intergenic
TTGAGACCGGCTGGGAATGA+TGG 0.453544 4_4:-13759683 None:intergenic
TGGTTCAAACAAATAGAAAA+CGG 0.454994 4_4:+13760325 Msa0670910:CDS
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4_4:+13759912 Msa0670910:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4_4:-13760024 None:intergenic
AATGTGGGGCTTGCACATAT+TGG 0.467947 4_4:+13760196 Msa0670910:CDS
TCAACATATGGGAACAAGTT+GGG 0.468173 4_4:-13761230 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.471359 4_4:-13760394 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4_4:+13760385 Msa0670910:CDS
TACTCACTCAATGCCGGAGC+AGG 0.473606 4_4:+13761094 Msa0670910:CDS
GCAATAACCTTGAGACCGGC+TGG 0.475576 4_4:-13759692 None:intergenic
AAAAGAAACACATGCAAAGA+AGG 0.478150 4_4:+13760371 Msa0670910:CDS
AAGTCATAAACATCCATGTC+TGG 0.491105 4_4:-13759970 None:intergenic
CACTTTCCTCAACTCTATTG+AGG 0.492388 4_4:+13759730 Msa0670910:CDS
CTATATGCTGTCAATCAACT+AGG 0.499504 4_4:+13760432 Msa0670910:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4_4:-13760455 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4_4:+13760180 Msa0670910:CDS
GTTCTGGCGACCAAGACAAA+CGG 0.513977 4_4:-13760395 None:intergenic
ATATGAAATTGTTGGAAGCT+CGG 0.515274 4_4:+13760302 Msa0670910:CDS
TGTAATCGATATCATTATCA+AGG 0.517908 4_4:+13759758 Msa0670910:CDS
GACAGGAATGTTGAGTTGTA+GGG 0.519779 4_4:-13760007 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4_4:+13759903 Msa0670910:CDS
TTATGAAGTCTATTGAATGT+GGG 0.532174 4_4:+13760181 Msa0670910:CDS
AAAACGAACCTGTAAATGGC+TGG 0.535893 4_4:-13760618 None:intergenic
CGACACACGACAACAATGGA+TGG 0.540785 4_4:+13761163 Msa0670910:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4_4:-13761241 None:intergenic
AGAAAACTCTGGTCCTGCTC+CGG 0.548712 4_4:-13761107 None:intergenic
ATCATTCCCAGCCGGTCTCA+AGG 0.552068 4_4:+13759685 Msa0670910:CDS
ATCAACATATGGGAACAAGT+TGG 0.563419 4_4:-13761231 None:intergenic
AGAAGCTACTCACTCAATGC+CGG 0.575449 4_4:+13761088 Msa0670910:CDS
TGAGACCGGCTGGGAATGAT+GGG 0.577892 4_4:-13759682 None:intergenic
CTTCATAATAGAACTTGCTG+TGG 0.579779 4_4:-13760166 None:intergenic
AATTGCAATAACCTTGAGAC+CGG 0.581075 4_4:-13759696 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.598969 4_4:+13760182 Msa0670910:CDS
ATTGCGACACACGACAACAA+TGG 0.611832 4_4:+13761159 Msa0670910:CDS
CAATAACCTTGAGACCGGCT+GGG 0.613963 4_4:-13759691 None:intergenic
CACAACCCATCATTCCCAGC+CGG 0.618478 4_4:+13759677 Msa0670910:CDS
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4_4:-13759736 None:intergenic
TCGAATTCAGGAGAAAACTC+TGG 0.627173 4_4:-13761118 None:intergenic
TGATGCTCATATGCCAGACA+TGG 0.629107 4_4:+13759957 Msa0670910:CDS
TCTTACTACATGTTGCCACA+TGG 0.630006 4_4:-13760250 None:intergenic
GGTCGCCAGAACTTCATCAG+CGG 0.636769 4_4:+13760406 Msa0670910:CDS
GCAATTGTCATGACATTGTG+TGG 0.655655 4_4:-13761141 None:intergenic
TGAAATTGTTGGAAGCTCGG+TGG 0.670504 4_4:+13760305 Msa0670910:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4_4:+13761255 Msa0670910:CDS
GTTCAAACAAATAGAAAACG+GGG 0.731666 4_4:+13760327 Msa0670910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATAGTAAAAAAGTAATT+AGG - chr4_4:13760478-13760497 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4_4:13760928-13760947 Msa0670910:intron 15.0%
!! CATATAGTAAAAAAGTAATT+AGG - chr4_4:13760478-13760497 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4_4:13760928-13760947 Msa0670910:intron 15.0%
!! AAAAGATGAAAACTTGAATA+AGG - chr4_4:13759801-13759820 None:intergenic 20.0%
!!! ATTCAAGTTTTCATCTTTTT+TGG + chr4_4:13759802-13759821 Msa0670910:intron 20.0%
!! CAATATAAGAATAAACAAAC+AGG - chr4_4:13759854-13759873 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13760027-13760046 None:intergenic 20.0%
!! AACAAAGAATATGAAATTGT+TGG + chr4_4:13760294-13760313 Msa0670910:CDS 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4_4:13760533-13760552 Msa0670910:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4_4:13760534-13760553 Msa0670910:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4_4:13760566-13760585 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4_4:13760565-13760584 Msa0670910:CDS 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG - chr4_4:13760597-13760616 None:intergenic 20.0%
!! AAAAATGTTGTTAGTAATGT+CGG - chr4_4:13761201-13761220 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4_4:13761244-13761263 None:intergenic 20.0%
!! AAAAGATGAAAACTTGAATA+AGG - chr4_4:13759801-13759820 None:intergenic 20.0%
!!! ATTCAAGTTTTCATCTTTTT+TGG + chr4_4:13759802-13759821 Msa0670910:intron 20.0%
!! CAATATAAGAATAAACAAAC+AGG - chr4_4:13759854-13759873 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4_4:13760027-13760046 None:intergenic 20.0%
!! AACAAAGAATATGAAATTGT+TGG + chr4_4:13760294-13760313 Msa0670910:CDS 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4_4:13760533-13760552 Msa0670910:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4_4:13760534-13760553 Msa0670910:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4_4:13760566-13760585 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4_4:13760565-13760584 Msa0670910:CDS 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG - chr4_4:13760597-13760616 None:intergenic 20.0%
!! AAAAATGTTGTTAGTAATGT+CGG - chr4_4:13761201-13761220 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4_4:13761244-13761263 None:intergenic 20.0%
! TGTAATCGATATCATTATCA+AGG + chr4_4:13759758-13759777 Msa0670910:CDS 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_4:13760134-13760153 Msa0670910:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_4:13760180-13760199 Msa0670910:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_4:13760181-13760200 Msa0670910:CDS 25.0%
! TGGTTCAAACAAATAGAAAA+CGG + chr4_4:13760325-13760344 Msa0670910:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4_4:13760535-13760554 Msa0670910:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG - chr4_4:13760596-13760615 None:intergenic 25.0%
! AAAATTACAACAACAGAGTT+AGG - chr4_4:13760722-13760741 None:intergenic 25.0%
! TTTAATAACATGCAAGACTA+AGG - chr4_4:13760768-13760787 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG - chr4_4:13760892-13760911 None:intergenic 25.0%
! GTAGAATGAAAAAATCAAAG+AGG + chr4_4:13761039-13761058 Msa0670910:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4_4:13761245-13761264 None:intergenic 25.0%
! TGTAATCGATATCATTATCA+AGG + chr4_4:13759758-13759777 Msa0670910:CDS 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_4:13760134-13760153 Msa0670910:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_4:13760180-13760199 Msa0670910:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_4:13760181-13760200 Msa0670910:CDS 25.0%
! TGGTTCAAACAAATAGAAAA+CGG + chr4_4:13760325-13760344 Msa0670910:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4_4:13760535-13760554 Msa0670910:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG - chr4_4:13760596-13760615 None:intergenic 25.0%
! AAAATTACAACAACAGAGTT+AGG - chr4_4:13760722-13760741 None:intergenic 25.0%
! TTTAATAACATGCAAGACTA+AGG - chr4_4:13760768-13760787 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG - chr4_4:13760892-13760911 None:intergenic 25.0%
! GTAGAATGAAAAAATCAAAG+AGG + chr4_4:13761039-13761058 Msa0670910:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4_4:13761245-13761264 None:intergenic 25.0%
TTCAGTTACCAAATGTAGTA+TGG + chr4_4:13759879-13759898 Msa0670910:intron 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_4:13760182-13760201 Msa0670910:CDS 30.0%
ATATGAAATTGTTGGAAGCT+CGG + chr4_4:13760302-13760321 Msa0670910:CDS 30.0%
GGTTCAAACAAATAGAAAAC+GGG + chr4_4:13760326-13760345 Msa0670910:CDS 30.0%
GTTCAAACAAATAGAAAACG+GGG + chr4_4:13760327-13760346 Msa0670910:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_4:13760371-13760390 Msa0670910:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_4:13760458-13760477 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+TGG - chr4_4:13760874-13760893 None:intergenic 30.0%
!!! CATTCTACCTTTTTTCTCTT+CGG - chr4_4:13761027-13761046 None:intergenic 30.0%
ACGCTAAATTGTGTTTCAAA+AGG - chr4_4:13761068-13761087 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4_4:13761255-13761274 Msa0670910:CDS 30.0%
TTCAGTTACCAAATGTAGTA+TGG + chr4_4:13759879-13759898 Msa0670910:intron 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_4:13760182-13760201 Msa0670910:CDS 30.0%
ATATGAAATTGTTGGAAGCT+CGG + chr4_4:13760302-13760321 Msa0670910:CDS 30.0%
GGTTCAAACAAATAGAAAAC+GGG + chr4_4:13760326-13760345 Msa0670910:CDS 30.0%
GTTCAAACAAATAGAAAACG+GGG + chr4_4:13760327-13760346 Msa0670910:CDS 30.0%
AAAAGAAACACATGCAAAGA+AGG + chr4_4:13760371-13760390 Msa0670910:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4_4:13760458-13760477 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+TGG - chr4_4:13760874-13760893 None:intergenic 30.0%
!!! CATTCTACCTTTTTTCTCTT+CGG - chr4_4:13761027-13761046 None:intergenic 30.0%
ACGCTAAATTGTGTTTCAAA+AGG - chr4_4:13761068-13761087 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4_4:13761255-13761274 Msa0670910:CDS 30.0%
AATTGCAATAACCTTGAGAC+CGG - chr4_4:13759699-13759718 None:intergenic 35.0%
ATCAGAAGCCATACTACATT+TGG - chr4_4:13759890-13759909 None:intergenic 35.0%
CTTTCTTTCCAACATGTTGA+AGG - chr4_4:13759914-13759933 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG + chr4_4:13759912-13759931 Msa0670910:CDS 35.0%
AAGTCATAAACATCCATGTC+TGG - chr4_4:13759973-13759992 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4_4:13759999-13760018 None:intergenic 35.0%
!! CTTCATAATAGAACTTGCTG+TGG - chr4_4:13760169-13760188 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4_4:13760221-13760240 Msa0670910:CDS 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4_4:13760228-13760247 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_4:13760370-13760389 None:intergenic 35.0%
CTATATGCTGTCAATCAACT+AGG + chr4_4:13760432-13760451 Msa0670910:CDS 35.0%
TGCTAAAACGAACCTGTAAA+TGG - chr4_4:13760625-13760644 None:intergenic 35.0%
AAAAGATCACTGACAATCCT+AGG - chr4_4:13760698-13760717 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4_4:13760804-13760823 Msa0670910:intron 35.0%
!!! TACCTTTTTTCTCTTCGGTT+GGG - chr4_4:13761022-13761041 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4_4:13761233-13761252 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4_4:13761234-13761253 None:intergenic 35.0%
AATTGCAATAACCTTGAGAC+CGG - chr4_4:13759699-13759718 None:intergenic 35.0%
ATCAGAAGCCATACTACATT+TGG - chr4_4:13759890-13759909 None:intergenic 35.0%
CTTTCTTTCCAACATGTTGA+AGG - chr4_4:13759914-13759933 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG + chr4_4:13759912-13759931 Msa0670910:CDS 35.0%
AAGTCATAAACATCCATGTC+TGG - chr4_4:13759973-13759992 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4_4:13759999-13760018 None:intergenic 35.0%
!! CTTCATAATAGAACTTGCTG+TGG - chr4_4:13760169-13760188 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4_4:13760221-13760240 Msa0670910:CDS 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4_4:13760228-13760247 None:intergenic 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4_4:13760370-13760389 None:intergenic 35.0%
CTATATGCTGTCAATCAACT+AGG + chr4_4:13760432-13760451 Msa0670910:CDS 35.0%
TGCTAAAACGAACCTGTAAA+TGG - chr4_4:13760625-13760644 None:intergenic 35.0%
AAAAGATCACTGACAATCCT+AGG - chr4_4:13760698-13760717 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4_4:13760804-13760823 Msa0670910:intron 35.0%
!!! TACCTTTTTTCTCTTCGGTT+GGG - chr4_4:13761022-13761041 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4_4:13761233-13761252 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4_4:13761234-13761253 None:intergenic 35.0%
CACTTTCCTCAACTCTATTG+AGG + chr4_4:13759730-13759749 Msa0670910:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_4:13759739-13759758 None:intergenic 40.0%
TTCTGATGCCTTCAACATGT+TGG + chr4_4:13759903-13759922 Msa0670910:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4_4:13760010-13760029 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4_4:13760011-13760030 None:intergenic 40.0%
CACATATTGGACTAAGCCTT+TGG + chr4_4:13760209-13760228 Msa0670910:CDS 40.0%
AAAAAGAGGTCAAGACCATG+TGG + chr4_4:13760235-13760254 Msa0670910:CDS 40.0%
TCTTACTACATGTTGCCACA+TGG - chr4_4:13760253-13760272 None:intergenic 40.0%
AAAACGAACCTGTAAATGGC+TGG - chr4_4:13760621-13760640 None:intergenic 40.0%
AATCCTAGGCAACTAGACTT+AGG - chr4_4:13760684-13760703 None:intergenic 40.0%
!!! CTACCTTTTTTCTCTTCGGT+TGG - chr4_4:13761023-13761042 None:intergenic 40.0%
TCGAATTCAGGAGAAAACTC+TGG - chr4_4:13761121-13761140 None:intergenic 40.0%
GCAATTGTCATGACATTGTG+TGG - chr4_4:13761144-13761163 None:intergenic 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4_4:13759730-13759749 Msa0670910:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_4:13759739-13759758 None:intergenic 40.0%
TTCTGATGCCTTCAACATGT+TGG + chr4_4:13759903-13759922 Msa0670910:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4_4:13760010-13760029 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4_4:13760011-13760030 None:intergenic 40.0%
CACATATTGGACTAAGCCTT+TGG + chr4_4:13760209-13760228 Msa0670910:CDS 40.0%
AAAAAGAGGTCAAGACCATG+TGG + chr4_4:13760235-13760254 Msa0670910:CDS 40.0%
TCTTACTACATGTTGCCACA+TGG - chr4_4:13760253-13760272 None:intergenic 40.0%
AAAACGAACCTGTAAATGGC+TGG - chr4_4:13760621-13760640 None:intergenic 40.0%
AATCCTAGGCAACTAGACTT+AGG - chr4_4:13760684-13760703 None:intergenic 40.0%
!!! CTACCTTTTTTCTCTTCGGT+TGG - chr4_4:13761023-13761042 None:intergenic 40.0%
TCGAATTCAGGAGAAAACTC+TGG - chr4_4:13761121-13761140 None:intergenic 40.0%
GCAATTGTCATGACATTGTG+TGG - chr4_4:13761144-13761163 None:intergenic 40.0%
TGATGCTCATATGCCAGACA+TGG + chr4_4:13759957-13759976 Msa0670910:CDS 45.0%
AATGTGGGGCTTGCACATAT+TGG + chr4_4:13760196-13760215 Msa0670910:CDS 45.0%
TGAAATTGTTGGAAGCTCGG+TGG + chr4_4:13760305-13760324 Msa0670910:CDS 45.0%
AGAAACCGCTGATGAAGTTC+TGG - chr4_4:13760414-13760433 None:intergenic 45.0%
ACATGTTGCCAGCCATTTAC+AGG + chr4_4:13760610-13760629 Msa0670910:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG + chr4_4:13760678-13760697 Msa0670910:intron 45.0%
AGCCCAACCGAAGAGAAAAA+AGG + chr4_4:13761017-13761036 Msa0670910:CDS 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4_4:13761088-13761107 Msa0670910:CDS 45.0%
GACATTGTGTGGTCGAATTC+AGG - chr4_4:13761133-13761152 None:intergenic 45.0%
ATTGCGACACACGACAACAA+TGG + chr4_4:13761159-13761178 Msa0670910:CDS 45.0%
TGATGCTCATATGCCAGACA+TGG + chr4_4:13759957-13759976 Msa0670910:CDS 45.0%
AATGTGGGGCTTGCACATAT+TGG + chr4_4:13760196-13760215 Msa0670910:CDS 45.0%
TGAAATTGTTGGAAGCTCGG+TGG + chr4_4:13760305-13760324 Msa0670910:CDS 45.0%
AGAAACCGCTGATGAAGTTC+TGG - chr4_4:13760414-13760433 None:intergenic 45.0%
ACATGTTGCCAGCCATTTAC+AGG + chr4_4:13760610-13760629 Msa0670910:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG + chr4_4:13760678-13760697 Msa0670910:intron 45.0%
AGCCCAACCGAAGAGAAAAA+AGG + chr4_4:13761017-13761036 Msa0670910:CDS 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4_4:13761088-13761107 Msa0670910:CDS 45.0%
GACATTGTGTGGTCGAATTC+AGG - chr4_4:13761133-13761152 None:intergenic 45.0%
ATTGCGACACACGACAACAA+TGG + chr4_4:13761159-13761178 Msa0670910:CDS 45.0%
CAATAACCTTGAGACCGGCT+GGG - chr4_4:13759694-13759713 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13760385-13760404 Msa0670910:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13760397-13760416 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_4:13760398-13760417 None:intergenic 50.0%
AGAAAACTCTGGTCCTGCTC+CGG - chr4_4:13761110-13761129 None:intergenic 50.0%
CGACACACGACAACAATGGA+TGG + chr4_4:13761163-13761182 Msa0670910:CDS 50.0%
CAATAACCTTGAGACCGGCT+GGG - chr4_4:13759694-13759713 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_4:13760385-13760404 Msa0670910:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_4:13760397-13760416 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4_4:13760398-13760417 None:intergenic 50.0%
AGAAAACTCTGGTCCTGCTC+CGG - chr4_4:13761110-13761129 None:intergenic 50.0%
CGACACACGACAACAATGGA+TGG + chr4_4:13761163-13761182 Msa0670910:CDS 50.0%
CACAACCCATCATTCCCAGC+CGG + chr4_4:13759677-13759696 Msa0670910:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4_4:13759685-13759704 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4_4:13759686-13759705 None:intergenic 55.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4_4:13759685-13759704 Msa0670910:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4_4:13759695-13759714 None:intergenic 55.0%
GGTCGCCAGAACTTCATCAG+CGG + chr4_4:13760406-13760425 Msa0670910:CDS 55.0%
TACTCACTCAATGCCGGAGC+AGG + chr4_4:13761094-13761113 Msa0670910:CDS 55.0%
CACAACCCATCATTCCCAGC+CGG + chr4_4:13759677-13759696 Msa0670910:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4_4:13759685-13759704 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4_4:13759686-13759705 None:intergenic 55.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4_4:13759685-13759704 Msa0670910:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4_4:13759695-13759714 None:intergenic 55.0%
GGTCGCCAGAACTTCATCAG+CGG + chr4_4:13760406-13760425 Msa0670910:CDS 55.0%
TACTCACTCAATGCCGGAGC+AGG + chr4_4:13761094-13761113 Msa0670910:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 13759662 13761280 13759662 ID=Msa0670910;Name=Msa0670910
chr4_4 mRNA 13759662 13761280 13759662 ID=Msa0670910-mRNA-1;Parent=Msa0670910;Name=Msa0670910-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0|1|1|1|5|0|313
chr4_4 exon 13759662 13759779 13759662 ID=Msa0670910-mRNA-1:exon:4182;Parent=Msa0670910-mRNA-1
chr4_4 exon 13759884 13760033 13759884 ID=Msa0670910-mRNA-1:exon:4183;Parent=Msa0670910-mRNA-1
chr4_4 exon 13760151 13760462 13760151 ID=Msa0670910-mRNA-1:exon:4184;Parent=Msa0670910-mRNA-1
chr4_4 exon 13760555 13760631 13760555 ID=Msa0670910-mRNA-1:exon:4185;Parent=Msa0670910-mRNA-1
chr4_4 exon 13760996 13761280 13760996 ID=Msa0670910-mRNA-1:exon:4186;Parent=Msa0670910-mRNA-1
chr4_4 CDS 13759662 13759779 13759662 ID=Msa0670910-mRNA-1:cds;Parent=Msa0670910-mRNA-1
chr4_4 CDS 13759884 13760033 13759884 ID=Msa0670910-mRNA-1:cds;Parent=Msa0670910-mRNA-1
chr4_4 CDS 13760151 13760462 13760151 ID=Msa0670910-mRNA-1:cds;Parent=Msa0670910-mRNA-1
chr4_4 CDS 13760555 13760631 13760555 ID=Msa0670910-mRNA-1:cds;Parent=Msa0670910-mRNA-1
chr4_4 CDS 13760996 13761280 13760996 ID=Msa0670910-mRNA-1:cds;Parent=Msa0670910-mRNA-1
Gene Sequence

>Msa0670910

ATGGCAGTTTTCACTCACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAGCAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCACATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGACCATGTGGCAACATGTAGTAAGAATTAGAAACTGTTTGACTGAAAACAAAGAATATGAAATTGTTGGAAGCTCGGTGGTTCAAACAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAACTTCATCAGCGGTTTCTATATGCTGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGAAGTATAGAAATTATTTAAAGAGCCCAACCGAAGAGAAAAAAGGTAGAATGAAAAAATCAAAGAGGAGTCCTTTTGAAACACAATTTAGCGTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCGACCACACAATGTCATGACAATTGCGACACACGACAACAATGGATGGAAATTAGTGAATCCGACATTACTAACAACATTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>Msa0670910

MAVFTHNPSFPAGLKVIAIDHDITFLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTASSIMKSIECGACTYWTKPLVEKEVKTMWQHVVRIRNCLTENKEYEIVGSSVVQTNRKRGREDANASKETHAKKARLSWSPELHQRFLYAVNQLGLDRATPKKILKIMDVPDMKKEHVASHLQKYRNYLKSPTEEKKGRMKKSKRSPFETQFSVEATHSMPEQDQSFLLNSTTQCHDNCDTRQQWMEISESDITNNIFSDLPNLFPYVDDDLSSLVW*