Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0671370 | AES61431.2 | 93.684 | 190 | 12 | 0 | 1 | 190 | 13 | 202 | 6.64e-130 | 376 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0671370 | G7I8T1 | 93.684 | 190 | 12 | 0 | 1 | 190 | 13 | 202 | 3.17e-130 | 376 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0671370 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0671370 | MtrunA17_Chr1g0172971 | 84.545 | 110 | 17 | 0 | 11 | 120 | 8 | 117 | 6.71e-62 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGCTGTTGGCCTGTTGTT+AGG | 0.299104 | 4_4:+14577075 | None:intergenic |
| TTACTTCTCGAAATGATCTA+AGG | 0.311482 | 4_4:+14576884 | None:intergenic |
| GCTCTTATAAATTCTAAAGT+TGG | 0.400120 | 4_4:-14576841 | Msa0671370:CDS |
| CTCCTAGCAATAAAGAATCA+TGG | 0.428271 | 4_4:-14577287 | Msa0671370:CDS |
| GAAAGGATTGGTTGTAAAGC+TGG | 0.447509 | 4_4:-14577006 | Msa0671370:CDS |
| ATGTGTATAAGATATATAGA+TGG | 0.452819 | 4_4:-14576954 | Msa0671370:CDS |
| CTAAGGTAACGCGAATGCTC+TGG | 0.476897 | 4_4:+14576901 | None:intergenic |
| TCCCATGATTCTTTATTGCT+AGG | 0.477301 | 4_4:+14577285 | None:intergenic |
| TTACAACCAATCCTTTCCTC+AGG | 0.480530 | 4_4:+14577012 | None:intergenic |
| GTTTGCTTAATCCTAACAAC+AGG | 0.484661 | 4_4:-14577086 | Msa0671370:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 4_4:-14577123 | Msa0671370:CDS |
| ACAAAACAAGAAATCTGATA+AGG | 0.511435 | 4_4:-14577157 | Msa0671370:CDS |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 4_4:-14577112 | Msa0671370:CDS |
| TCCTAGCAATAAAGAATCAT+GGG | 0.512680 | 4_4:-14577286 | Msa0671370:CDS |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 4_4:-14577219 | Msa0671370:CDS |
| AGCTGGAATATATTTGAAGA+TGG | 0.525650 | 4_4:-14576989 | Msa0671370:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 4_4:-14577111 | Msa0671370:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 4_4:-14576947 | Msa0671370:CDS |
| GAATGCTCTGGTTTATGTAA+TGG | 0.573489 | 4_4:+14576913 | None:intergenic |
| TGTGTATAAGATATATAGAT+GGG | 0.601002 | 4_4:-14576953 | Msa0671370:CDS |
| ACCAGTGGACTGTCTGCTGT+TGG | 0.602965 | 4_4:+14577063 | None:intergenic |
| GCCAACAGCAGACAGTCCAC+TGG | 0.611268 | 4_4:-14577064 | Msa0671370:CDS |
| TTCACAAACTCAATAACCAG+TGG | 0.633415 | 4_4:+14577048 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAGATTGAAGATTTTTA+TGG | - | chr4_4:14576978-14576997 | Msa0671370:CDS | 20.0% |
| !! | ATGTGTATAAGATATATAGA+TGG | - | chr4_4:14577264-14577283 | Msa0671370:CDS | 20.0% |
| !! | TGTGTATAAGATATATAGAT+GGG | - | chr4_4:14577265-14577284 | Msa0671370:CDS | 20.0% |
| !! | ATAACACTGGATTTTCTATT+AGG | - | chr4_4:14577012-14577031 | Msa0671370:CDS | 25.0% |
| ! | ACAAAACAAGAAATCTGATA+AGG | - | chr4_4:14577061-14577080 | Msa0671370:CDS | 25.0% |
| !!! | GCTCTTATAAATTCTAAAGT+TGG | - | chr4_4:14577377-14577396 | Msa0671370:CDS | 25.0% |
| TCCTAGCAATAAAGAATCAT+GGG | - | chr4_4:14576932-14576951 | Msa0671370:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | - | chr4_4:14576999-14577018 | Msa0671370:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | chr4_4:14577095-14577114 | Msa0671370:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | chr4_4:14577106-14577125 | Msa0671370:CDS | 30.0% | |
| AACAAACAAGGTTTCAAGAA+GGG | - | chr4_4:14577107-14577126 | Msa0671370:CDS | 30.0% | |
| AGCTGGAATATATTTGAAGA+TGG | - | chr4_4:14577229-14577248 | Msa0671370:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | chr4_4:14577271-14577290 | Msa0671370:CDS | 30.0% | |
| TTACTTCTCGAAATGATCTA+AGG | + | chr4_4:14577337-14577356 | None:intergenic | 30.0% | |
| CTCCTAGCAATAAAGAATCA+TGG | - | chr4_4:14576931-14576950 | Msa0671370:CDS | 35.0% | |
| TCCCATGATTCTTTATTGCT+AGG | + | chr4_4:14576936-14576955 | None:intergenic | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | chr4_4:14577132-14577151 | Msa0671370:CDS | 35.0% | |
| TTCACAAACTCAATAACCAG+TGG | + | chr4_4:14577173-14577192 | None:intergenic | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | - | chr4_4:14577190-14577209 | Msa0671370:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | - | chr4_4:14577195-14577214 | Msa0671370:CDS | 35.0% | |
| ! | GAATGCTCTGGTTTATGTAA+TGG | + | chr4_4:14577308-14577327 | None:intergenic | 35.0% |
| GAAAAACCTGAGGAAAGGAT+TGG | - | chr4_4:14577200-14577219 | Msa0671370:CDS | 40.0% | |
| TTACAACCAATCCTTTCCTC+AGG | + | chr4_4:14577209-14577228 | None:intergenic | 40.0% | |
| !! | GAAAGGATTGGTTGTAAAGC+TGG | - | chr4_4:14577212-14577231 | Msa0671370:CDS | 40.0% |
| ! | TCTGCTGTTGGCCTGTTGTT+AGG | + | chr4_4:14577146-14577165 | None:intergenic | 50.0% |
| CTAAGGTAACGCGAATGCTC+TGG | + | chr4_4:14577320-14577339 | None:intergenic | 50.0% | |
| !! | ACCAGTGGACTGTCTGCTGT+TGG | + | chr4_4:14577158-14577177 | None:intergenic | 55.0% |
| GCCAACAGCAGACAGTCCAC+TGG | - | chr4_4:14577154-14577173 | Msa0671370:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 14576834 | 14577406 | 14576834 | ID=Msa0671370;Name=Msa0671370 |
| chr4_4 | mRNA | 14576834 | 14577406 | 14576834 | ID=Msa0671370-mRNA-1;Parent=Msa0671370;Name=Msa0671370-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|190 |
| chr4_4 | exon | 14576834 | 14577406 | 14576834 | ID=Msa0671370-mRNA-1:exon:4477;Parent=Msa0671370-mRNA-1 |
| chr4_4 | CDS | 14576834 | 14577406 | 14576834 | ID=Msa0671370-mRNA-1:cds;Parent=Msa0671370-mRNA-1 |
| Gene Sequence |
| Protein sequence |
