AlfalfaGEDB | Msa0671370

M. sativa cultivar zhongmu-4 / Msa0671370

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0671370	AES61431.2	93.684	190	12	0	1	190	13	202	6.64e-130	376

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0671370	G7I8T1	93.684	190	12	0	1	190	13	202	3.17e-130	376

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TFs/TRs:

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Gene ID	Type	Classification
Msa0671370	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0671370	MtrunA17_Chr1g0172971	84.545	110	17	0	11	120	8	117	6.71e-62	201

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAACAAGGTTTCAAGAA+GGG	0.554561	4_4:-14577111	Msa0671370:CDS
ACAAAACAAGAAATCTGATA+AGG	0.511435	4_4:-14577157	Msa0671370:CDS
ACCAGTGGACTGTCTGCTGT+TGG	0.602965	4_4:+14577063	None:intergenic
AGCTGGAATATATTTGAAGA+TGG	0.525650	4_4:-14576989	Msa0671370:CDS
ATGTGTATAAGATATATAGA+TGG	0.452819	4_4:-14576954	Msa0671370:CDS
CGTTATGTTTGTAACAAACA+AGG	0.494754	4_4:-14577123	Msa0671370:CDS
CTAAGGTAACGCGAATGCTC+TGG	0.476897	4_4:+14576901	None:intergenic
CTCCTAGCAATAAAGAATCA+TGG	0.428271	4_4:-14577287	Msa0671370:CDS
GAAAGGATTGGTTGTAAAGC+TGG	0.447509	4_4:-14577006	Msa0671370:CDS
GAATGCTCTGGTTTATGTAA+TGG	0.573489	4_4:+14576913	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCTAGCAATAAAGAATCAT+GGG	-	chr4_4:14576932-14576951	Msa0671370:CDS	30.0%
GGTAATTATGCATATAACAC+TGG	-	chr4_4:14576999-14577018	Msa0671370:CDS	30.0%
CGTTATGTTTGTAACAAACA+AGG	-	chr4_4:14577095-14577114	Msa0671370:CDS	30.0%
TAACAAACAAGGTTTCAAGA+AGG	-	chr4_4:14577106-14577125	Msa0671370:CDS	30.0%
AACAAACAAGGTTTCAAGAA+GGG	-	chr4_4:14577107-14577126	Msa0671370:CDS	30.0%
AGCTGGAATATATTTGAAGA+TGG	-	chr4_4:14577229-14577248	Msa0671370:CDS	30.0%
TAAGATATATAGATGGGATG+TGG	-	chr4_4:14577271-14577290	Msa0671370:CDS	30.0%
TTACTTCTCGAAATGATCTA+AGG	+	chr4_4:14577337-14577356	None:intergenic	30.0%
CTCCTAGCAATAAAGAATCA+TGG	-	chr4_4:14576931-14576950	Msa0671370:CDS	35.0%
TCCCATGATTCTTTATTGCT+AGG	+	chr4_4:14576936-14576955	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	14576834	14577406	14576834	ID=Msa0671370;Name=Msa0671370
chr4_4	mRNA	14576834	14577406	14576834	ID=Msa0671370-mRNA-1;Parent=Msa0671370;Name=Msa0671370-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|190
chr4_4	exon	14576834	14577406	14576834	ID=Msa0671370-mRNA-1:exon:4477;Parent=Msa0671370-mRNA-1
chr4_4	CDS	14576834	14577406	14576834	ID=Msa0671370-mRNA-1:cds;Parent=Msa0671370-mRNA-1

Gene Sequence

>Msa0671370

CTGAGTGTTGAAACTTCATCACTGATTCATGCTGAGAGTAGACAATTGAGTGTTGAAAGTTCTTCAATGATTCATGCTGACAGTTCTCAATGTTACTCTCCTAGCAATAAAGAATCATGGGAGTCTTTAGTCTTTCAATCTATTAAAGAGATTGAAGATTTTTATGGTAATTATGCATATAACACTGGATTTTCTATTAGGACTATGTTGAAGTCGAAGAATAATTCACAAAACAAGAAATCTGATAAGGTACATTATGTGCGTTATGTTTGTAACAAACAAGGTTTCAAGAAGGGAAGTTTGCTTAATCCTAACAACAGGCCAACAGCAGACAGTCCACTGGTTATTGAGTTTGTGAAAGTGAAAGAAAAACCTGAGGAAAGGATTGGTTGTAAAGCTGGAATATATTTGAAGATGGATGAAGTTTTGAATGTGTATAAGATATATAGATGGGATGTGGCTCACTGTCATCCATTACATAAACCAGAGCATTCGCGTTACCTTAGATCATTTCGAGAAGTAAATGAAGTTCAACGACAACTAGCTCTTATAAATTCTAAAGTTGGAATGTAG

Protein sequence

>Msa0671370

LSVETSSLIHAESRQLSVESSSMIHADSSQCYSPSNKESWESLVFQSIKEIEDFYGNYAYNTGFSIRTMLKSKNNSQNKKSDKVHYVRYVCNKQGFKKGSLLNPNNRPTADSPLVIEFVKVKEKPEERIGCKAGIYLKMDEVLNVYKIYRWDVAHCHPLHKPEHSRYLRSFREVNEVQRQLALINSKVGM*