AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0672200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0672200 MtrunA17_Chr4g0012071 94.195 534 29 2 93 625 20 552 0.0 1019
Msa0672200 MtrunA17_Chr7g0230771 60.829 434 154 5 199 625 308 732 3.87e-167 494
Msa0672200 MtrunA17_Chr7g0230771 35.610 205 102 9 1 189 4 194 1.79e-12 70.5
Msa0672200 MtrunA17_Chr7g0230731 60.369 434 156 5 199 625 305 729 2.23e-164 487
Msa0672200 MtrunA17_Chr8g0373561 33.514 370 228 10 267 625 180 542 1.86e-52 189
Msa0672200 MtrunA17_Chr7g0224271 27.934 426 256 14 230 625 270 674 2.61e-32 132
Msa0672200 MtrunA17_Chr8g0376381 26.415 371 237 8 283 624 159 522 3.43e-32 130
Msa0672200 MtrunA17_Chr4g0038761 28.142 366 236 9 274 624 89 442 2.36e-31 127
Msa0672200 MtrunA17_Chr2g0306641 25.604 414 263 11 231 625 101 488 9.16e-31 126
Msa0672200 MtrunA17_Chr5g0445331 26.525 377 249 10 270 624 162 532 3.27e-30 125
Msa0672200 MtrunA17_Chr4g0039661 29.639 388 222 15 266 624 190 555 1.26e-29 123
Msa0672200 MtrunA17_Chr3g0110971 25.729 377 249 8 270 624 220 587 1.78e-29 123
Msa0672200 MtrunA17_Chr3g0101141 25.000 420 262 14 234 624 147 542 4.27e-29 122
Msa0672200 MtrunA17_Chr5g0447691 24.865 370 260 8 270 624 178 544 6.74e-29 121
Msa0672200 MtrunA17_Chr7g0245601 26.558 369 234 8 274 624 443 792 6.91e-28 119
Msa0672200 MtrunA17_Chr2g0290241 25.330 379 254 11 268 625 56 426 2.63e-27 115
Msa0672200 MtrunA17_Chr2g0319091 25.641 351 219 9 300 623 243 578 7.27e-26 112
Msa0672200 MtrunA17_Chr4g0076091 24.274 379 254 10 270 625 176 544 3.39e-24 107
Msa0672200 MtrunA17_Chr4g0057291 25.263 380 247 11 270 623 293 661 4.01e-24 107
Msa0672200 MtrunA17_Chr1g0183641 24.725 364 235 11 283 625 133 478 9.85e-24 105
Msa0672200 MtrunA17_Chr3g0125811 25.000 372 257 8 270 624 204 570 3.38e-23 103
Msa0672200 MtrunA17_Chr1g0159461 26.260 377 246 12 270 624 226 592 4.77e-23 103
Msa0672200 MtrunA17_Chr5g0403741 26.724 232 156 7 401 625 51 275 4.44e-22 96.7
Msa0672200 MtrunA17_Chr4g0057891 24.937 397 260 13 256 625 136 521 9.80e-22 99.4
Msa0672200 MtrunA17_Chr5g0424131 26.087 391 242 16 270 625 122 500 1.01e-20 95.9
Msa0672200 MtrunA17_Chr3g0114841 27.575 301 201 10 337 625 206 501 1.86e-20 95.1
Msa0672200 MtrunA17_Chr1g0200401 27.523 327 177 15 339 624 152 459 5.43e-20 93.2
Msa0672200 MtrunA17_Chr7g0270031 22.844 429 295 10 228 624 130 554 9.23e-19 90.1
Msa0672200 MtrunA17_Chr2g0328381 25.067 375 250 12 270 623 276 640 2.16e-18 89.4
Msa0672200 MtrunA17_Chr2g0294471 25.459 381 259 12 265 624 200 576 1.05e-17 87.0
Msa0672200 MtrunA17_Chr8g0383981 26.440 382 241 16 263 623 133 495 1.43e-17 86.3
Msa0672200 MtrunA17_Chr2g0328431 22.222 414 289 10 232 623 251 653 4.57e-17 85.1
Msa0672200 MtrunA17_Chr7g0238891 21.958 378 254 9 274 624 377 740 1.14e-16 84.0
Msa0672200 MtrunA17_Chr2g0294461 25.000 384 260 12 265 624 206 585 1.49e-16 83.2
Msa0672200 MtrunA17_Chr2g0328341 23.262 374 258 10 270 623 273 637 2.88e-16 82.4
Msa0672200 MtrunA17_Chr2g0328391 21.774 372 262 8 274 623 304 668 1.01e-15 80.9
Msa0672200 MtrunA17_Chr2g0328411 21.831 426 295 12 222 623 242 653 6.99e-15 78.2
Msa0672200 MtrunA17_Chr8g0357601 21.662 397 268 10 258 624 169 552 3.71e-14 75.5
Msa0672200 MtrunA17_Chr5g0401131 20.844 379 273 7 270 624 115 490 4.28e-14 75.1
Msa0672200 MtrunA17_Chr4g0031741 22.281 377 258 10 270 623 285 649 4.56e-14 75.5
Msa0672200 MtrunA17_Chr2g0328371 24.706 255 171 7 337 577 2 249 1.19e-13 71.2
Msa0672200 MtrunA17_Chr2g0328361 23.243 370 238 14 270 623 275 614 2.99e-13 72.8
Msa0672200 MtrunA17_Chr4g0031711 25.899 278 182 7 270 529 362 633 4.23e-13 72.4
Msa0672200 MtrunA17_Chr4g0031701 24.910 277 186 7 270 529 255 526 2.01e-12 70.1
Msa0672200 MtrunA17_Chr2g0329591 21.680 369 247 10 288 624 93 451 2.72e-12 69.3
Msa0672200 MtrunA17_Chr4g0031731 24.549 277 187 7 270 529 255 526 3.35e-12 69.7
Msa0672200 MtrunA17_Chr2g0328401 21.818 220 158 5 229 440 250 463 4.15e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0672200 AT4G00150.1 41.746 630 284 17 4 625 1 555 8.91e-133 400
Msa0672200 AT2G45160.1 39.822 673 321 24 4 625 1 640 9.65e-111 346
Msa0672200 AT3G60630.1 40.000 660 321 25 4 625 1 623 6.91e-110 343
Msa0672200 AT4G36710.1 31.649 376 224 11 268 624 124 485 4.25e-51 184
Msa0672200 AT2G04890.1 27.957 372 239 9 270 624 54 413 1.11e-35 139
Msa0672200 AT5G66770.1 28.756 386 250 13 256 624 207 584 3.89e-34 137
Msa0672200 AT2G01570.1 26.630 368 246 9 270 624 225 581 3.49e-30 125
Msa0672200 AT5G17490.1 26.854 391 257 11 247 624 142 516 1.80e-29 123
Msa0672200 AT3G03450.1 27.397 292 198 5 343 624 258 545 1.37e-28 120
Msa0672200 AT5G48150.1 23.873 377 270 8 263 624 116 490 1.61e-28 120
Msa0672200 AT5G48150.4 23.873 377 270 8 263 624 116 490 1.61e-28 120
Msa0672200 AT5G48150.2 23.873 377 270 8 263 624 116 490 1.61e-28 120
Msa0672200 AT5G48150.3 23.873 377 270 8 263 624 116 490 1.61e-28 120
Msa0672200 AT3G54220.1 26.648 364 241 7 274 623 298 649 1.90e-28 121
Msa0672200 AT3G50650.1 27.132 387 241 12 262 624 173 542 5.81e-28 119
Msa0672200 AT4G17230.1 26.509 381 254 10 263 624 152 525 9.20e-28 118
Msa0672200 AT4G17230.2 26.509 381 254 10 263 624 152 525 9.20e-28 118
Msa0672200 AT1G14920.1 24.518 363 260 6 270 624 173 529 2.12e-27 117
Msa0672200 AT1G50600.3 23.389 419 264 11 236 624 135 526 7.62e-27 115
Msa0672200 AT1G66350.1 26.871 294 192 8 343 625 226 507 8.76e-27 115
Msa0672200 AT1G50600.1 23.301 412 273 10 236 624 206 597 2.72e-26 114
Msa0672200 AT1G50600.2 23.301 412 273 10 236 624 210 601 3.62e-26 114
Msa0672200 AT1G21450.1 24.595 370 262 7 270 624 226 593 4.53e-26 113
Msa0672200 AT5G41920.1 24.599 374 246 9 270 624 44 400 1.09e-24 107
Msa0672200 AT1G63100.2 25.326 383 240 13 275 623 283 653 3.31e-21 98.6
Msa0672200 AT1G63100.1 25.326 383 240 13 275 623 258 628 3.99e-21 98.2
Msa0672200 AT4G08250.1 26.578 301 202 10 337 623 181 476 3.53e-19 91.3
Msa0672200 AT1G55580.1 24.590 305 215 6 335 624 141 445 2.57e-18 88.6
Msa0672200 AT5G59450.1 22.252 373 270 7 270 624 228 598 5.80e-18 88.2
Msa0672200 AT4G37650.1 19.792 384 278 8 270 624 147 529 3.78e-15 79.0
Msa0672200 AT3G46600.1 23.256 344 238 8 300 624 243 579 1.01e-14 77.8
Msa0672200 AT3G46600.3 23.167 341 242 8 300 624 211 547 1.31e-14 77.4
Msa0672200 AT3G46600.2 23.167 341 242 8 300 624 35 371 1.49e-14 76.3
Msa0672200 AT1G07520.3 25.743 202 139 5 274 466 324 523 3.53e-13 73.2
Msa0672200 AT1G07520.1 25.743 202 139 5 274 466 323 522 3.62e-13 73.2
Msa0672200 AT1G07520.2 25.743 202 139 5 274 466 159 358 3.73e-13 72.8
Msa0672200 AT1G07520.4 25.743 202 139 5 274 466 159 358 3.73e-13 72.8
Msa0672200 AT1G50420.1 28.497 193 121 4 270 449 58 246 1.50e-11 67.4
Msa0672200 AT2G29065.1 21.658 374 266 10 274 623 257 627 6.08e-11 65.9
Msa0672200 AT2G29060.1 20.912 373 273 10 270 623 322 691 7.49e-11 65.9
Msa0672200 AT2G29060.2 20.912 373 273 10 270 623 285 654 9.63e-11 65.5

Find 150 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAAGAATCCTCTCTTTA+TGG 0.092963 4_4:+16478459 Msa0672200:three_prime_UTR
CCTAATGTTTGTTAACTAAA+TGG 0.126757 4_4:+16478322 Msa0672200:three_prime_UTR
TTACACAACAATACACTTTA+AGG 0.131559 4_4:+16475870 Msa0672200:five_prime_UTR
AAAATAAAGATTGAGTATTT+TGG 0.160504 4_4:-16475593 None:intergenic
TGTAATGCCTTTCAATCTTT+TGG 0.183368 4_4:-16477943 None:intergenic
AATTGCGTGCTGAGATATTT+TGG 0.187631 4_4:-16475684 None:intergenic
ATCAACCTCAACAACAAAAT+TGG 0.235662 4_4:+16476442 Msa0672200:CDS
GTCTTGTCTTTCTTGATCTA+AGG 0.247486 4_4:+16478486 Msa0672200:three_prime_UTR
GATAAGTGGCTAATTATTAA+AGG 0.259137 4_4:+16478212 Msa0672200:exon
AGACAAGGCATAGTAATTTA+TGG 0.269109 4_4:-16475458 None:intergenic
GTGTTACCGCTCTTGGATTC+TGG 0.279830 4_4:+16476927 Msa0672200:CDS
TTGAAATTCCTCAAAAGTTA+AGG 0.300516 4_4:-16476349 None:intergenic
CCATTTAGTTAACAAACATT+AGG 0.311269 4_4:-16478322 None:intergenic
GACAATGTTGAAGATGAAAA+TGG 0.331833 4_4:-16476537 None:intergenic
TCCAAAGTTACGACCACTTT+CGG 0.339309 4_4:-16477806 None:intergenic
TTAAAGGTTGAAATTTCTTA+AGG 0.345571 4_4:+16478228 Msa0672200:three_prime_UTR
TTGCAATGTGTTACCGCTCT+TGG 0.347688 4_4:+16476920 Msa0672200:CDS
TAAACACTTGATCGAGTGTT+TGG 0.352030 4_4:+16478550 Msa0672200:three_prime_UTR
ATTGTTTACAGAAACTGATT+TGG 0.353647 4_4:-16475490 None:intergenic
TGATGGATTACTTATGAAAC+TGG 0.357205 4_4:-16477744 None:intergenic
CATTTAGTTAACAAACATTA+GGG 0.374065 4_4:-16478321 None:intergenic
TCTTGATCTAAGGCTGTGTT+TGG 0.374269 4_4:+16478496 Msa0672200:three_prime_UTR
TAGTAAGAAATGATCAGTTT+AGG 0.380521 4_4:+16478430 Msa0672200:three_prime_UTR
CTTCCTCTCTTGTGCTATGC+TGG 0.380612 4_4:+16478149 Msa0672200:CDS
CTAATGTTTGTTAACTAAAT+GGG 0.381359 4_4:+16478323 Msa0672200:three_prime_UTR
ACAATAATAGAAACTAATTA+TGG 0.382308 4_4:-16475633 None:intergenic
TGAAATTCCTCAAAAGTTAA+GGG 0.383228 4_4:-16476348 None:intergenic
GGTTCAAGCGCAAGGGATAT+TGG 0.384808 4_4:+16477181 Msa0672200:CDS
ACTAATTATGGTTATGATAA+GGG 0.386415 4_4:-16475621 None:intergenic
TGAATGAAGCATTAACTGTA+AGG 0.398966 4_4:-16477273 None:intergenic
AACTAATTATGGTTATGATA+AGG 0.416365 4_4:-16475622 None:intergenic
AGCTGAGTGTTTGGTTCAAA+GGG 0.419565 4_4:+16478093 Msa0672200:CDS
AATTAGCTGTAATTTGTGTT+TGG 0.426950 4_4:+16478603 Msa0672200:three_prime_UTR
GGAAGCTCAAGCTGAGTGTT+TGG 0.430829 4_4:+16478084 Msa0672200:CDS
CAAATATACTCTGCTGAAGC+TGG 0.434387 4_4:-16477115 None:intergenic
ATTTCAAGTGAAGGGAATGT+TGG 0.436223 4_4:+16476365 Msa0672200:CDS
TTACTTATGAAACTGGAAAC+AGG 0.436478 4_4:-16477737 None:intergenic
AATATCCCTTGCGCTTGAAC+CGG 0.438162 4_4:-16477179 None:intergenic
CTTTGAAAAGGCTTACCAAT+TGG 0.441810 4_4:-16477227 None:intergenic
GCCGAAAGTGGTCGTAACTT+TGG 0.444136 4_4:+16477805 Msa0672200:CDS
TCAAGCCCTCATTGAAGCCT+TGG 0.450274 4_4:+16477409 Msa0672200:CDS
AAGCTGAGTGTTTGGTTCAA+AGG 0.455607 4_4:+16478092 Msa0672200:CDS
GAATGTGAAGGGAGAGAAAC+CGG 0.460030 4_4:-16478050 None:intergenic
TTGCAATTCTCAGGAATGAA+TGG 0.464532 4_4:-16476903 None:intergenic
TGGTATGAGAATGGAGGATT+GGG 0.465608 4_4:+16476680 Msa0672200:CDS
TAGAAAGAAGCCCTTTGAAA+AGG 0.471306 4_4:-16477239 None:intergenic
AAGTTGGCAAACTGAAGAAC+AGG 0.472756 4_4:-16477377 None:intergenic
AACTCATCTCAGTTTCAACT+TGG 0.473456 4_4:+16478182 Msa0672200:CDS
CTTCACATTCAGCAAATTCA+CGG 0.474423 4_4:+16478063 Msa0672200:CDS
GAAGATGAAAATGGAGAACT+AGG 0.474745 4_4:-16476528 None:intergenic
AAGGTTGAAATTTCTTAAGG+TGG 0.475998 4_4:+16478231 Msa0672200:three_prime_UTR
ATGGTTGAACATGGACGCTT+CGG 0.477095 4_4:+16476854 Msa0672200:CDS
AAAGAACAAGGACAAAGCTT+AGG 0.481534 4_4:-16475523 None:intergenic
AGTTAACAAACATTAGGGTA+TGG 0.486346 4_4:-16478316 None:intergenic
CTTGCCATCATGTCCTCTAC+CGG 0.490539 4_4:+16477673 Msa0672200:CDS
GGCTTGATTACAAGTAAAGT+TGG 0.491191 4_4:-16477393 None:intergenic
CAGTTAATGCTTCATTCAAA+CGG 0.491869 4_4:+16477278 Msa0672200:CDS
TGGTGGTGGTGGTATGAGAA+TGG 0.492769 4_4:+16476671 Msa0672200:CDS
TGCTCAGATATCAATGTGGC+AGG 0.493264 4_4:-16475546 None:intergenic
GCTGATAAAAGCTGGTAATC+CGG 0.495893 4_4:+16477160 Msa0672200:CDS
TTTGAGGAATTTCAAGTGAA+GGG 0.497110 4_4:+16476357 Msa0672200:CDS
AGGAACATGTTTCTTCAGTC+CGG 0.498389 4_4:+16478031 Msa0672200:CDS
GGTAACACATTGCAATTCTC+AGG 0.500098 4_4:-16476912 None:intergenic
AATCACCAGCTCTTACCAAT+TGG 0.501286 4_4:+16477212 Msa0672200:CDS
GTGGTATGAGAATGGAGGAT+TGG 0.504718 4_4:+16476679 Msa0672200:CDS
TTCAAATAGCAATACTTCAA+TGG 0.504902 4_4:+16476593 Msa0672200:CDS
TTTGATAGTAATGAAGCCAT+TGG 0.505073 4_4:+16477704 Msa0672200:CDS
GTAATTTGTCACGCTGGTTG+TGG 0.506339 4_4:-16478000 None:intergenic
CTGAAGAACAGGTGATATCT+CGG 0.507582 4_4:-16477366 None:intergenic
TCCAATTGCGAAGCAGCAAA+AGG 0.508239 4_4:+16477058 Msa0672200:CDS
GATGTTGAAGACCCTTATGC+TGG 0.508840 4_4:+16476741 Msa0672200:CDS
TCTTGAATCAAGAACTGAAG+TGG 0.511894 4_4:-16476499 None:intergenic
CAACCGACGACTGCTGTTGT+TGG 0.513401 4_4:-16477034 None:intergenic
AACTTGGAGATGTTGATAAG+TGG 0.518300 4_4:+16478198 Msa0672200:exon
TCCACTTGAAATCCTCTCAC+AGG 0.520356 4_4:-16478118 None:intergenic
CTGGTCAAGAAGTGTTTGCT+AGG 0.526401 4_4:+16476946 Msa0672200:CDS
CTCAATCTCGTTGCAAGACG+AGG 0.528947 4_4:-16477561 None:intergenic
CTGGATGCTTGCATCATCTC+CGG 0.537282 4_4:-16477096 None:intergenic
ACTGCTGAGCTGATAAAAGC+TGG 0.542424 4_4:+16477152 Msa0672200:CDS
TGGGAAGAGGAACGTCCATT+CGG 0.544546 4_4:-16477841 None:intergenic
TTGTCATCTTCTCATGGTAG+TGG 0.544818 4_4:+16476555 Msa0672200:CDS
ATCTTGCAAAGTGGTGGTTG+TGG 0.551252 4_4:+16476774 Msa0672200:CDS
TGCTAGGAGACATCAGCAGC+AGG 0.551740 4_4:+16476962 Msa0672200:CDS
GGTAATCCGGTTCAAGCGCA+AGG 0.552039 4_4:+16477173 Msa0672200:CDS
TCAACATTGTCATCTTCTCA+TGG 0.552805 4_4:+16476549 Msa0672200:CDS
CTCTTGTGCTATGCTGGCAG+AGG 0.553514 4_4:+16478155 Msa0672200:CDS
GCTTACCAATTGGTAAGAGC+TGG 0.555163 4_4:-16477217 None:intergenic
GGTAGTGGTAGTAATGGTAA+TGG 0.556024 4_4:+16476570 Msa0672200:CDS
TGAATGACATCGGTGGGAAG+AGG 0.559908 4_4:-16477854 None:intergenic
AAAGCAGCTAAGGCCGAAAG+TGG 0.562649 4_4:+16477793 Msa0672200:CDS
CCTCTCCAAGGCTTCAATGA+GGG 0.564144 4_4:-16477414 None:intergenic
CAGCATAGCACAAGAGAGGA+AGG 0.564647 4_4:-16478148 None:intergenic
ATGTACTCCAAAAGATTGAA+AGG 0.570089 4_4:+16477936 Msa0672200:CDS
GTTCAAAGGGCACCTGTGAG+AGG 0.570717 4_4:+16478106 Msa0672200:CDS
AGAGCCACAGGGAAGCATGA+TGG 0.571215 4_4:-16477761 None:intergenic
CATCATTTGAAAGATGAGCA+AGG 0.573001 4_4:-16476629 None:intergenic
TCATGATGATGAGGAAACAA+TGG 0.573020 4_4:-16476996 None:intergenic
AAGGTGAGTTCTATTACTAC+CGG 0.573317 4_4:+16477077 Msa0672200:CDS
TCTCATGGTAGTGGTAGTAA+TGG 0.576556 4_4:+16476564 Msa0672200:CDS
CGTGAATTTGCTGAATGTGA+AGG 0.578961 4_4:-16478062 None:intergenic
ACCATCAACAAAATCATCCT+CGG 0.579390 4_4:+16477974 Msa0672200:CDS
TGAAGAAACATGTTCCTCCA+TGG 0.581165 4_4:-16478025 None:intergenic
TCAAAGAAATGACCCGGTAG+AGG 0.583745 4_4:-16477686 None:intergenic
CTTGAATCAAGAACTGAAGT+GGG 0.584678 4_4:-16476498 None:intergenic
TGGATCATCAACAACAACAA+TGG 0.586807 4_4:+16476835 Msa0672200:CDS
AACAAAATCTTGCAAAGTGG+TGG 0.589673 4_4:+16476768 Msa0672200:CDS
GTGAATTTGCTGAATGTGAA+GGG 0.591860 4_4:-16478061 None:intergenic
TTCAAACGGTAACAATCTCA+CGG 0.592949 4_4:+16477292 Msa0672200:CDS
AACAGGAAAGTTCACACCAA+TGG 0.593516 4_4:-16477720 None:intergenic
CCCTCATTGAAGCCTTGGAG+AGG 0.594395 4_4:+16477414 Msa0672200:CDS
AGCGTGACAAATTACCTCCA+TGG 0.597025 4_4:+16478008 Msa0672200:CDS
GAGATTGTACTAAGAAGTAA+CGG 0.599321 4_4:+16477503 Msa0672200:CDS
AGAACATGAATGACATCGGT+GGG 0.602707 4_4:-16477860 None:intergenic
ACCTCTCCAAGGCTTCAATG+AGG 0.608874 4_4:-16477415 None:intergenic
TGGTGGTGGTATGAGAATGG+AGG 0.610885 4_4:+16476674 Msa0672200:CDS
TAAAGAAGATTATTGCTATG+TGG 0.612360 4_4:+16476473 Msa0672200:CDS
ACAACAACAATGGTTGAACA+TGG 0.613222 4_4:+16476845 Msa0672200:CDS
TTGCCATCATGTCCTCTACC+GGG 0.614713 4_4:+16477674 Msa0672200:CDS
TGACCCGGTAGAGGACATGA+TGG 0.615427 4_4:-16477677 None:intergenic
CAATGTGGCAGGAAAGAACA+AGG 0.624770 4_4:-16475535 None:intergenic
AATTCGATCGAACCTCTCCA+AGG 0.628009 4_4:-16477426 None:intergenic
ATGAGAATGGAGGATTGGGA+AGG 0.628687 4_4:+16476684 Msa0672200:CDS
ATGGAGGTAATTTGTCACGC+TGG 0.629161 4_4:-16478006 None:intergenic
AAGATGAAAATGGAGAACTA+GGG 0.633076 4_4:-16476527 None:intergenic
AAGAACATGAATGACATCGG+TGG 0.635028 4_4:-16477861 None:intergenic
ACAGCAGTAATCTTAAGTGA+AGG 0.636223 4_4:-16477530 None:intergenic
GTAATCCGGTTCAAGCGCAA+GGG 0.638818 4_4:+16477174 Msa0672200:CDS
CTGCCAGCATAGCACAAGAG+AGG 0.640951 4_4:-16478152 None:intergenic
CAAGACTAAACCATAAAGAG+AGG 0.643558 4_4:-16478469 None:intergenic
TCAATCTCGTTGCAAGACGA+GGG 0.646604 4_4:-16477560 None:intergenic
ATATTGGGTTTGGAGTACAA+TGG 0.648294 4_4:+16477465 Msa0672200:CDS
AAGCGCAAGGGATATTGGCG+CGG 0.648472 4_4:+16477186 Msa0672200:CDS
AGATGGATGCGTGCATACGT+AGG 0.650132 4_4:-16475713 None:intergenic
TGAACTCAAACGACATGTTG+AGG 0.652289 4_4:-16477625 None:intergenic
ACCTGTGAGAGGATTTCAAG+TGG 0.654432 4_4:+16478117 Msa0672200:CDS
TGCTGCAGATCATGATGATG+AGG 0.657062 4_4:-16477005 None:intergenic
TTGAAGAACATGAATGACAT+CGG 0.658361 4_4:-16477864 None:intergenic
ATGGACGCTTCGGTTCAACA+AGG 0.660128 4_4:+16476864 Msa0672200:CDS
TTACTATCAAAGAAATGACC+CGG 0.669847 4_4:-16477692 None:intergenic
CACGCTGGTTGTGGTGACCG+AGG 0.670125 4_4:-16477991 None:intergenic
TGGACGCTTCGGTTCAACAA+GGG 0.687015 4_4:+16476865 Msa0672200:CDS
ATTTGGACATGTCAGAGACA+AGG 0.688777 4_4:-16475473 None:intergenic
GATAACAGAGAAACAGTGAA+CGG 0.690073 4_4:-16478386 None:intergenic
TTACCAACAACAGCAGTCGT+CGG 0.697756 4_4:+16477031 Msa0672200:CDS
AGAAACATGTTCCTCCATGG+AGG 0.701239 4_4:-16478022 None:intergenic
TAATCCATCATGCTTCCCTG+TGG 0.706732 4_4:+16477757 Msa0672200:CDS
TTGAACAAAATCTTGCAAAG+TGG 0.719051 4_4:+16476765 Msa0672200:CDS
TCTTGACCAGAATCCAAGAG+CGG 0.742204 4_4:-16476933 None:intergenic
AAAATGCTCAGATATCAATG+TGG 0.751076 4_4:-16475550 None:intergenic
GTGACAAATTACCTCCATGG+AGG 0.762900 4_4:+16478011 Msa0672200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGATGATTTTTTTTATT+GGG - chr4_4:16476014-16476033 None:intergenic 10.0%
!!! AAAAAGATGATTTTTTTTAT+TGG - chr4_4:16476015-16476034 None:intergenic 10.0%
!!! ATTTTAAAAATGAAACTAAT+CGG - chr4_4:16477326-16477345 None:intergenic 10.0%
!!! ATTAGTTTCATTTTTAAAAT+TGG + chr4_4:16477326-16477345 Msa0672200:CDS 10.0%
!!! AAAATAAAGATTGAGTATTT+TGG - chr4_4:16475596-16475615 None:intergenic 15.0%
!! ACAATAATAGAAACTAATTA+TGG - chr4_4:16475636-16475655 None:intergenic 15.0%
!!! AAAGATGATTTTTTTTATTG+GGG - chr4_4:16476013-16476032 None:intergenic 15.0%
!!! AAAATCATCTTTTTTTCATA+AGG + chr4_4:16476021-16476040 Msa0672200:intron 15.0%
!!! AAATCATCTTTTTTTCATAA+GGG + chr4_4:16476022-16476041 Msa0672200:intron 15.0%
!!! ATGTTATTGATTTTGATATT+GGG + chr4_4:16477450-16477469 Msa0672200:CDS 15.0%
!!! ATTGTTTGTGTAATTTTTTT+AGG + chr4_4:16478279-16478298 Msa0672200:three_prime_UTR 15.0%
!! ACTAATTATGGTTATGATAA+GGG - chr4_4:16475624-16475643 None:intergenic 20.0%
!! AACTAATTATGGTTATGATA+AGG - chr4_4:16475625-16475644 None:intergenic 20.0%
!!! TTTTTTTTATTGGGGTATTA+GGG - chr4_4:16476005-16476024 None:intergenic 20.0%
!!! ATTTTTTTTATTGGGGTATT+AGG - chr4_4:16476006-16476025 None:intergenic 20.0%
!!! TTGAAATTAAATCTGTTTTC+TGG + chr4_4:16476159-16476178 Msa0672200:intron 20.0%
!!! TGAAATTAAATCTGTTTTCT+GGG + chr4_4:16476160-16476179 Msa0672200:intron 20.0%
!!! CATGTTATTGATTTTGATAT+TGG + chr4_4:16477449-16477468 Msa0672200:CDS 20.0%
!! TTAAAGGTTGAAATTTCTTA+AGG + chr4_4:16478228-16478247 Msa0672200:three_prime_UTR 20.0%
!! CATTTAGTTAACAAACATTA+GGG - chr4_4:16478324-16478343 None:intergenic 20.0%
!!! CTAATGTTTGTTAACTAAAT+GGG + chr4_4:16478323-16478342 Msa0672200:three_prime_UTR 20.0%
! ATTGTTTACAGAAACTGATT+TGG - chr4_4:16475493-16475512 None:intergenic 25.0%
! TTACACAACAATACACTTTA+AGG + chr4_4:16475870-16475889 Msa0672200:five_prime_UTR 25.0%
!!! TTTTTTCCTTTTAGCCAAAT+GGG - chr4_4:16476112-16476131 None:intergenic 25.0%
!!! TTTTTTTCCTTTTAGCCAAA+TGG - chr4_4:16476113-16476132 None:intergenic 25.0%
!! GAAATTAAATCTGTTTTCTG+GGG + chr4_4:16476161-16476180 Msa0672200:intron 25.0%
! TTCTTTGTTCTTGTTCAAAT+TGG + chr4_4:16476295-16476314 Msa0672200:intron 25.0%
!!! ACTTTTTCGTTTTTAGATGA+AGG + chr4_4:16476320-16476339 Msa0672200:intron 25.0%
! TGAAATTCCTCAAAAGTTAA+GGG - chr4_4:16476351-16476370 None:intergenic 25.0%
! TTGAAATTCCTCAAAAGTTA+AGG - chr4_4:16476352-16476371 None:intergenic 25.0%
!!! AATAACCAATTTTGTTGTTG+AGG - chr4_4:16476450-16476469 None:intergenic 25.0%
! TAAAGAAGATTATTGCTATG+TGG + chr4_4:16476473-16476492 Msa0672200:CDS 25.0%
! TTCAAATAGCAATACTTCAA+TGG + chr4_4:16476593-16476612 Msa0672200:CDS 25.0%
!!! TCACTTTTGAGGTTAATTAT+GGG + chr4_4:16476717-16476736 Msa0672200:CDS 25.0%
!!! TCTCAAAATGTTGATTTTCA+TGG + chr4_4:16476798-16476817 Msa0672200:CDS 25.0%
!!! ATTGATTTTGATATTGGGTT+TGG + chr4_4:16477455-16477474 Msa0672200:CDS 25.0%
!!! ATACTGACTCAAATTTTCTT+GGG - chr4_4:16477597-16477616 None:intergenic 25.0%
!!! TTCATTTTTTAAAGCAGCTA+AGG + chr4_4:16477783-16477802 Msa0672200:CDS 25.0%
! GATAAGTGGCTAATTATTAA+AGG + chr4_4:16478212-16478231 Msa0672200:exon 25.0%
! CCATTTAGTTAACAAACATT+AGG - chr4_4:16478325-16478344 None:intergenic 25.0%
!! CCTAATGTTTGTTAACTAAA+TGG + chr4_4:16478322-16478341 Msa0672200:three_prime_UTR 25.0%
! TAGTAAGAAATGATCAGTTT+AGG + chr4_4:16478430-16478449 Msa0672200:three_prime_UTR 25.0%
! AATTAAGAATCCTCTCTTTA+TGG + chr4_4:16478459-16478478 Msa0672200:three_prime_UTR 25.0%
AGACAAGGCATAGTAATTTA+TGG - chr4_4:16475461-16475480 None:intergenic 30.0%
AAAATGCTCAGATATCAATG+TGG - chr4_4:16475553-16475572 None:intergenic 30.0%
!! GTGTTGTGTTTTTGTTAAGA+TGG - chr4_4:16475733-16475752 None:intergenic 30.0%
! GAACAAAGGTGTAAACTTTA+TGG + chr4_4:16476216-16476235 Msa0672200:intron 30.0%
!!! TTATGGTCTTTTGAGACTTT+TGG + chr4_4:16476233-16476252 Msa0672200:intron 30.0%
!!! TATGGTCTTTTGAGACTTTT+GGG + chr4_4:16476234-16476253 Msa0672200:intron 30.0%
!! TTTTGAGGAATTTCAAGTGA+AGG + chr4_4:16476356-16476375 Msa0672200:CDS 30.0%
! TTTGAGGAATTTCAAGTGAA+GGG + chr4_4:16476357-16476376 Msa0672200:CDS 30.0%
ATCAACCTCAACAACAAAAT+TGG + chr4_4:16476442-16476461 Msa0672200:CDS 30.0%
AAGATGAAAATGGAGAACTA+GGG - chr4_4:16476530-16476549 None:intergenic 30.0%
GACAATGTTGAAGATGAAAA+TGG - chr4_4:16476540-16476559 None:intergenic 30.0%
GAGAATAGTGAAGAAAAATG+TGG + chr4_4:16476651-16476670 Msa0672200:CDS 30.0%
AATAGTGAAGAAAAATGTGG+TGG + chr4_4:16476654-16476673 Msa0672200:CDS 30.0%
!!! GTCACTTTTGAGGTTAATTA+TGG + chr4_4:16476716-16476735 Msa0672200:CDS 30.0%
! TTTTGTTCAATCCAGCATAA+GGG - chr4_4:16476755-16476774 None:intergenic 30.0%
! ATTTTGTTCAATCCAGCATA+AGG - chr4_4:16476756-16476775 None:intergenic 30.0%
TTGAACAAAATCTTGCAAAG+TGG + chr4_4:16476765-16476784 Msa0672200:CDS 30.0%
!!! CAAAATGTTGATTTTCATGG+TGG + chr4_4:16476801-16476820 Msa0672200:CDS 30.0%
! AATTGGTAAGCCTTTTCAAA+GGG + chr4_4:16477229-16477248 Msa0672200:CDS 30.0%
! TGAATGAAGCATTAACTGTA+AGG - chr4_4:16477276-16477295 None:intergenic 30.0%
CAGTTAATGCTTCATTCAAA+CGG + chr4_4:16477278-16477297 Msa0672200:CDS 30.0%
GAGATTGTACTAAGAAGTAA+CGG + chr4_4:16477503-16477522 Msa0672200:CDS 30.0%
!!! CATACTGACTCAAATTTTCT+TGG - chr4_4:16477598-16477617 None:intergenic 30.0%
TTACTATCAAAGAAATGACC+CGG - chr4_4:16477695-16477714 None:intergenic 30.0%
! TTTGATAGTAATGAAGCCAT+TGG + chr4_4:16477704-16477723 Msa0672200:CDS 30.0%
TTACTTATGAAACTGGAAAC+AGG - chr4_4:16477740-16477759 None:intergenic 30.0%
!! TGATGGATTACTTATGAAAC+TGG - chr4_4:16477747-16477766 None:intergenic 30.0%
TTAAAAAATGAAGAGCCACA+GGG - chr4_4:16477775-16477794 None:intergenic 30.0%
TTTAAAAAATGAAGAGCCAC+AGG - chr4_4:16477776-16477795 None:intergenic 30.0%
TTGAAGAACATGAATGACAT+CGG - chr4_4:16477867-16477886 None:intergenic 30.0%
ATGTACTCCAAAAGATTGAA+AGG + chr4_4:16477936-16477955 Msa0672200:CDS 30.0%
! TGTAATGCCTTTCAATCTTT+TGG - chr4_4:16477946-16477965 None:intergenic 30.0%
AAGGTTGAAATTTCTTAAGG+TGG + chr4_4:16478231-16478250 Msa0672200:three_prime_UTR 30.0%
AGTTAACAAACATTAGGGTA+TGG - chr4_4:16478319-16478338 None:intergenic 30.0%
AAAGAACAAGGACAAAGCTT+AGG - chr4_4:16475526-16475545 None:intergenic 35.0%
! AATTGCGTGCTGAGATATTT+TGG - chr4_4:16475687-16475706 None:intergenic 35.0%
ATCAAGATTCAAGACCCATT+TGG + chr4_4:16476095-16476114 Msa0672200:intron 35.0%
!! TTTGAGACTTTTGGGTCATA+TGG + chr4_4:16476242-16476261 Msa0672200:intron 35.0%
!! TTGAGACTTTTGGGTCATAT+GGG + chr4_4:16476243-16476262 Msa0672200:intron 35.0%
!! TTTTGGGTCATATGGGTTAT+TGG + chr4_4:16476250-16476269 Msa0672200:intron 35.0%
!!! CATATGGGTTATTGGTTTTG+TGG + chr4_4:16476258-16476277 Msa0672200:intron 35.0%
! ATTTCAAGTGAAGGGAATGT+TGG + chr4_4:16476365-16476384 Msa0672200:CDS 35.0%
CTTGAATCAAGAACTGAAGT+GGG - chr4_4:16476501-16476520 None:intergenic 35.0%
TCTTGAATCAAGAACTGAAG+TGG - chr4_4:16476502-16476521 None:intergenic 35.0%
GAAGATGAAAATGGAGAACT+AGG - chr4_4:16476531-16476550 None:intergenic 35.0%
TCAACATTGTCATCTTCTCA+TGG + chr4_4:16476549-16476568 Msa0672200:CDS 35.0%
CATCATTTGAAAGATGAGCA+AGG - chr4_4:16476632-16476651 None:intergenic 35.0%
AACAAAATCTTGCAAAGTGG+TGG + chr4_4:16476768-16476787 Msa0672200:CDS 35.0%
!! GTTGATTTTCATGGTGGTTT+TGG + chr4_4:16476807-16476826 Msa0672200:CDS 35.0%
TGGATCATCAACAACAACAA+TGG + chr4_4:16476835-16476854 Msa0672200:CDS 35.0%
ACAACAACAATGGTTGAACA+TGG + chr4_4:16476845-16476864 Msa0672200:CDS 35.0%
TTGCAATTCTCAGGAATGAA+TGG - chr4_4:16476906-16476925 None:intergenic 35.0%
! TCATGATGATGAGGAAACAA+TGG - chr4_4:16476999-16477018 None:intergenic 35.0%
AAGGTGAGTTCTATTACTAC+CGG + chr4_4:16477077-16477096 Msa0672200:CDS 35.0%
! CTTTGAAAAGGCTTACCAAT+TGG - chr4_4:16477230-16477249 None:intergenic 35.0%
! CAATTGGTAAGCCTTTTCAA+AGG + chr4_4:16477228-16477247 Msa0672200:CDS 35.0%
! TAGAAAGAAGCCCTTTGAAA+AGG - chr4_4:16477242-16477261 None:intergenic 35.0%
TTCAAACGGTAACAATCTCA+CGG + chr4_4:16477292-16477311 Msa0672200:CDS 35.0%
GGCTTGATTACAAGTAAAGT+TGG - chr4_4:16477396-16477415 None:intergenic 35.0%
! ATATTGGGTTTGGAGTACAA+TGG + chr4_4:16477465-16477484 Msa0672200:CDS 35.0%
ACAGCAGTAATCTTAAGTGA+AGG - chr4_4:16477533-16477552 None:intergenic 35.0%
!!! TGATTTTGTTGATGGTTGGT+TGG - chr4_4:16477970-16477989 None:intergenic 35.0%
!!! AGGATGATTTTGTTGATGGT+TGG - chr4_4:16477974-16477993 None:intergenic 35.0%
ACCATCAACAAAATCATCCT+CGG + chr4_4:16477974-16477993 Msa0672200:CDS 35.0%
!! GTGAATTTGCTGAATGTGAA+GGG - chr4_4:16478064-16478083 None:intergenic 35.0%
CTTCACATTCAGCAAATTCA+CGG + chr4_4:16478063-16478082 Msa0672200:CDS 35.0%
AACTCATCTCAGTTTCAACT+TGG + chr4_4:16478182-16478201 Msa0672200:CDS 35.0%
! AACTTGGAGATGTTGATAAG+TGG + chr4_4:16478198-16478217 Msa0672200:exon 35.0%
GATAACAGAGAAACAGTGAA+CGG - chr4_4:16478389-16478408 None:intergenic 35.0%
CAAGACTAAACCATAAAGAG+AGG - chr4_4:16478472-16478491 None:intergenic 35.0%
GTCTTGTCTTTCTTGATCTA+AGG + chr4_4:16478486-16478505 Msa0672200:three_prime_UTR 35.0%
TAAACACTTGATCGAGTGTT+TGG + chr4_4:16478550-16478569 Msa0672200:three_prime_UTR 35.0%
ATTTGGACATGTCAGAGACA+AGG - chr4_4:16475476-16475495 None:intergenic 40.0%
TCAAGACCCATTTGGCTAAA+AGG + chr4_4:16476103-16476122 Msa0672200:intron 40.0%
!!! AAATCTGTTTTCTGGGGTAG+TGG + chr4_4:16476167-16476186 Msa0672200:intron 40.0%
AGAGGGTAAGAAGAGAACAA+AGG + chr4_4:16476202-16476221 Msa0672200:intron 40.0%
TTGTCATCTTCTCATGGTAG+TGG + chr4_4:16476555-16476574 Msa0672200:CDS 40.0%
!! TCTCATGGTAGTGGTAGTAA+TGG + chr4_4:16476564-16476583 Msa0672200:CDS 40.0%
! GGTAGTGGTAGTAATGGTAA+TGG + chr4_4:16476570-16476589 Msa0672200:CDS 40.0%
AGTGAAGAAAAATGTGGTGG+TGG + chr4_4:16476657-16476676 Msa0672200:CDS 40.0%
TGGTATGAGAATGGAGGATT+GGG + chr4_4:16476680-16476699 Msa0672200:CDS 40.0%
!!! GTCAAGATCAGTCACTTTTG+AGG + chr4_4:16476706-16476725 Msa0672200:CDS 40.0%
!!! TCATGGTGGTTTTGGAGTTT+TGG + chr4_4:16476815-16476834 Msa0672200:CDS 40.0%
GGTAACACATTGCAATTCTC+AGG - chr4_4:16476915-16476934 None:intergenic 40.0%
CAAATATACTCTGCTGAAGC+TGG - chr4_4:16477118-16477137 None:intergenic 40.0%
GCTGATAAAAGCTGGTAATC+CGG + chr4_4:16477160-16477179 Msa0672200:CDS 40.0%
AATCACCAGCTCTTACCAAT+TGG + chr4_4:16477212-16477231 Msa0672200:CDS 40.0%
CTGAAGAACAGGTGATATCT+CGG - chr4_4:16477369-16477388 None:intergenic 40.0%
AAGTTGGCAAACTGAAGAAC+AGG - chr4_4:16477380-16477399 None:intergenic 40.0%
TGAACTCAAACGACATGTTG+AGG - chr4_4:16477628-16477647 None:intergenic 40.0%
AACAGGAAAGTTCACACCAA+TGG - chr4_4:16477723-16477742 None:intergenic 40.0%
TCCAAAGTTACGACCACTTT+CGG - chr4_4:16477809-16477828 None:intergenic 40.0%
AGAACATGAATGACATCGGT+GGG - chr4_4:16477863-16477882 None:intergenic 40.0%
AAGAACATGAATGACATCGG+TGG - chr4_4:16477864-16477883 None:intergenic 40.0%
!!! CAATCTTTTGGAGTACATCG+AGG - chr4_4:16477934-16477953 None:intergenic 40.0%
! ACCGAGGATGATTTTGTTGA+TGG - chr4_4:16477978-16477997 None:intergenic 40.0%
TGAAGAAACATGTTCCTCCA+TGG - chr4_4:16478028-16478047 None:intergenic 40.0%
AGGAACATGTTTCTTCAGTC+CGG + chr4_4:16478031-16478050 Msa0672200:CDS 40.0%
!! CGTGAATTTGCTGAATGTGA+AGG - chr4_4:16478065-16478084 None:intergenic 40.0%
! AAGCTGAGTGTTTGGTTCAA+AGG + chr4_4:16478092-16478111 Msa0672200:CDS 40.0%
! AGCTGAGTGTTTGGTTCAAA+GGG + chr4_4:16478093-16478112 Msa0672200:CDS 40.0%
! TCTTGATCTAAGGCTGTGTT+TGG + chr4_4:16478496-16478515 Msa0672200:three_prime_UTR 40.0%
CAATGTGGCAGGAAAGAACA+AGG - chr4_4:16475538-16475557 None:intergenic 45.0%
TGCTCAGATATCAATGTGGC+AGG - chr4_4:16475549-16475568 None:intergenic 45.0%
!!! TTCTGGGGTAGTGGATTTTG+TGG + chr4_4:16476176-16476195 Msa0672200:intron 45.0%
!!! TCTGGGGTAGTGGATTTTGT+GGG + chr4_4:16476177-16476196 Msa0672200:intron 45.0%
!!! TAGTGGATTTTGTGGGGAAG+AGG + chr4_4:16476184-16476203 Msa0672200:intron 45.0%
!! AGTGGATTTTGTGGGGAAGA+GGG + chr4_4:16476185-16476204 Msa0672200:intron 45.0%
! GGCTTTGCCCTTAACTTTTG+AGG + chr4_4:16476341-16476360 Msa0672200:CDS 45.0%
!! GAAGAAAAATGTGGTGGTGG+TGG + chr4_4:16476660-16476679 Msa0672200:CDS 45.0%
GTGGTATGAGAATGGAGGAT+TGG + chr4_4:16476679-16476698 Msa0672200:CDS 45.0%
ATGAGAATGGAGGATTGGGA+AGG + chr4_4:16476684-16476703 Msa0672200:CDS 45.0%
GATGTTGAAGACCCTTATGC+TGG + chr4_4:16476741-16476760 Msa0672200:CDS 45.0%
ATCTTGCAAAGTGGTGGTTG+TGG + chr4_4:16476774-16476793 Msa0672200:CDS 45.0%
ATGGTTGAACATGGACGCTT+CGG + chr4_4:16476854-16476873 Msa0672200:CDS 45.0%
TTGCAATGTGTTACCGCTCT+TGG + chr4_4:16476920-16476939 Msa0672200:CDS 45.0%
TCTTGACCAGAATCCAAGAG+CGG - chr4_4:16476936-16476955 None:intergenic 45.0%
!! CTGGTCAAGAAGTGTTTGCT+AGG + chr4_4:16476946-16476965 Msa0672200:CDS 45.0%
! TGCTGCAGATCATGATGATG+AGG - chr4_4:16477008-16477027 None:intergenic 45.0%
TTACCAACAACAGCAGTCGT+CGG + chr4_4:16477031-16477050 Msa0672200:CDS 45.0%
TCCAATTGCGAAGCAGCAAA+AGG + chr4_4:16477058-16477077 Msa0672200:CDS 45.0%
!! ACCTTTTGCTGCTTCGCAAT+TGG - chr4_4:16477062-16477081 None:intergenic 45.0%
ACTGCTGAGCTGATAAAAGC+TGG + chr4_4:16477152-16477171 Msa0672200:CDS 45.0%
AATATCCCTTGCGCTTGAAC+CGG - chr4_4:16477182-16477201 None:intergenic 45.0%
GCTTACCAATTGGTAAGAGC+TGG - chr4_4:16477220-16477239 None:intergenic 45.0%
AATTCGATCGAACCTCTCCA+AGG - chr4_4:16477429-16477448 None:intergenic 45.0%
TCAATCTCGTTGCAAGACGA+GGG - chr4_4:16477563-16477582 None:intergenic 45.0%
TCAAAGAAATGACCCGGTAG+AGG - chr4_4:16477689-16477708 None:intergenic 45.0%
TAATCCATCATGCTTCCCTG+TGG + chr4_4:16477757-16477776 Msa0672200:CDS 45.0%
! GTAATTTGTCACGCTGGTTG+TGG - chr4_4:16478003-16478022 None:intergenic 45.0%
! ATGGAGGTAATTTGTCACGC+TGG - chr4_4:16478009-16478028 None:intergenic 45.0%
AGCGTGACAAATTACCTCCA+TGG + chr4_4:16478008-16478027 Msa0672200:CDS 45.0%
GTGACAAATTACCTCCATGG+AGG + chr4_4:16478011-16478030 Msa0672200:CDS 45.0%
AGAAACATGTTCCTCCATGG+AGG - chr4_4:16478025-16478044 None:intergenic 45.0%
GAATGTGAAGGGAGAGAAAC+CGG - chr4_4:16478053-16478072 None:intergenic 45.0%
ACCTGTGAGAGGATTTCAAG+TGG + chr4_4:16478117-16478136 Msa0672200:CDS 45.0%
TCCACTTGAAATCCTCTCAC+AGG - chr4_4:16478121-16478140 None:intergenic 45.0%
!!! TGTTTTTTAAATTTTTTAAT+GGG - chr4_4:16475662-16475681 None:intergenic 5.0%
!!! TTGTTTTTTAAATTTTTTAA+TGG - chr4_4:16475663-16475682 None:intergenic 5.0%
AGATGGATGCGTGCATACGT+AGG - chr4_4:16475716-16475735 None:intergenic 50.0%
!!! CTGGGGTAGTGGATTTTGTG+GGG + chr4_4:16476178-16476197 Msa0672200:intron 50.0%
!! TGGTGGTGGTGGTATGAGAA+TGG + chr4_4:16476671-16476690 Msa0672200:CDS 50.0%
!! TGGTGGTGGTATGAGAATGG+AGG + chr4_4:16476674-16476693 Msa0672200:CDS 50.0%
ATGGACGCTTCGGTTCAACA+AGG + chr4_4:16476864-16476883 Msa0672200:CDS 50.0%
TGGACGCTTCGGTTCAACAA+GGG + chr4_4:16476865-16476884 Msa0672200:CDS 50.0%
GTGTTACCGCTCTTGGATTC+TGG + chr4_4:16476927-16476946 Msa0672200:CDS 50.0%
CTGGATGCTTGCATCATCTC+CGG - chr4_4:16477099-16477118 None:intergenic 50.0%
GTAATCCGGTTCAAGCGCAA+GGG + chr4_4:16477174-16477193 Msa0672200:CDS 50.0%
GGTTCAAGCGCAAGGGATAT+TGG + chr4_4:16477181-16477200 Msa0672200:CDS 50.0%
TCAAGCCCTCATTGAAGCCT+TGG + chr4_4:16477409-16477428 Msa0672200:CDS 50.0%
CCTCTCCAAGGCTTCAATGA+GGG - chr4_4:16477417-16477436 None:intergenic 50.0%
ACCTCTCCAAGGCTTCAATG+AGG - chr4_4:16477418-16477437 None:intergenic 50.0%
CTCAATCTCGTTGCAAGACG+AGG - chr4_4:16477564-16477583 None:intergenic 50.0%
CTTGCCATCATGTCCTCTAC+CGG + chr4_4:16477673-16477692 Msa0672200:CDS 50.0%
TTGCCATCATGTCCTCTACC+GGG + chr4_4:16477674-16477693 Msa0672200:CDS 50.0%
AAAGCAGCTAAGGCCGAAAG+TGG + chr4_4:16477793-16477812 Msa0672200:CDS 50.0%
GCCGAAAGTGGTCGTAACTT+TGG + chr4_4:16477805-16477824 Msa0672200:CDS 50.0%
GGACAAAAACTGCGACCGAA+TGG + chr4_4:16477826-16477845 Msa0672200:CDS 50.0%
TGGGAAGAGGAACGTCCATT+CGG - chr4_4:16477844-16477863 None:intergenic 50.0%
TGAATGACATCGGTGGGAAG+AGG - chr4_4:16477857-16477876 None:intergenic 50.0%
GGAAGCTCAAGCTGAGTGTT+TGG + chr4_4:16478084-16478103 Msa0672200:CDS 50.0%
CAGCATAGCACAAGAGAGGA+AGG - chr4_4:16478151-16478170 None:intergenic 50.0%
CTTCCTCTCTTGTGCTATGC+TGG + chr4_4:16478149-16478168 Msa0672200:CDS 50.0%
TGCTAGGAGACATCAGCAGC+AGG + chr4_4:16476962-16476981 Msa0672200:CDS 55.0%
CAACCGACGACTGCTGTTGT+TGG - chr4_4:16477037-16477056 None:intergenic 55.0%
GGTAATCCGGTTCAAGCGCA+AGG + chr4_4:16477173-16477192 Msa0672200:CDS 55.0%
AAGCGCAAGGGATATTGGCG+CGG + chr4_4:16477186-16477205 Msa0672200:CDS 55.0%
CCCTCATTGAAGCCTTGGAG+AGG + chr4_4:16477414-16477433 Msa0672200:CDS 55.0%
TGACCCGGTAGAGGACATGA+TGG - chr4_4:16477680-16477699 None:intergenic 55.0%
! AGAGCCACAGGGAAGCATGA+TGG - chr4_4:16477764-16477783 None:intergenic 55.0%
!! GTTCAAAGGGCACCTGTGAG+AGG + chr4_4:16478106-16478125 Msa0672200:CDS 55.0%
CTGCCAGCATAGCACAAGAG+AGG - chr4_4:16478155-16478174 None:intergenic 55.0%
CTCTTGTGCTATGCTGGCAG+AGG + chr4_4:16478155-16478174 Msa0672200:CDS 55.0%
! CACGCTGGTTGTGGTGACCG+AGG - chr4_4:16477994-16478013 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 16475441 16478625 16475441 ID=Msa0672200;Name=Msa0672200
chr4_4 mRNA 16475441 16478625 16475441 ID=Msa0672200-mRNA-1;Parent=Msa0672200;Name=Msa0672200-mRNA-1;_AED=0.00;_eAED=0.00;_QI=451|1|1|1|0|0.5|2|412|625
chr4_4 exon 16475441 16475891 16475441 ID=Msa0672200-mRNA-1:exon:4923;Parent=Msa0672200-mRNA-1
chr4_4 exon 16476336 16478625 16476336 ID=Msa0672200-mRNA-1:exon:4924;Parent=Msa0672200-mRNA-1
chr4_4 five_prime_UTR 16475441 16475891 16475441 ID=Msa0672200-mRNA-1:five_prime_utr;Parent=Msa0672200-mRNA-1
chr4_4 CDS 16476336 16478213 16476336 ID=Msa0672200-mRNA-1:cds;Parent=Msa0672200-mRNA-1
chr4_4 three_prime_UTR 16478214 16478625 16478214 ID=Msa0672200-mRNA-1:three_prime_utr;Parent=Msa0672200-mRNA-1
Gene Sequence

>Msa0672200

ATGAAGGCTTTGCCCTTAACTTTTGAGGAATTTCAAGTGAAGGGAATGTTGGATAATTTCTCTTCAACTTCAAATTCAGTTTCAGATTCATTTTCATTGCTTTTGAATCAACCTCAACAACAAAATTGGTTATTAGATAAAGAAGATTATTGCTATGTGGAGCCCACTTCAGTTCTTGATTCAAGAAGAAGCCCTAGTTCTCCATTTTCATCTTCAACATTGTCATCTTCTCATGGTAGTGGTAGTAATGGTAATGGTTCAAATAGCAATACTTCAATGGTAGTTTTTGAGAACCTTGCTCATCTTTCAAATGATGAGAATAGTGAAGAAAAATGTGGTGGTGGTGGTATGAGAATGGAGGATTGGGAAGGTCAAGATCAGTCACTTTTGAGGTTAATTATGGGAGATGTTGAAGACCCTTATGCTGGATTGAACAAAATCTTGCAAAGTGGTGGTTGTGGTTCTCAAAATGTTGATTTTCATGGTGGTTTTGGAGTTTTGGATCATCAACAACAACAATGGTTGAACATGGACGCTTCGGTTCAACAAGGGAATTACAATGTTTTTCCATTCATTCCTGAGAATTGCAATGTGTTACCGCTCTTGGATTCTGGTCAAGAAGTGTTTGCTAGGAGACATCAGCAGCAGGAAACACAGCTTCCATTGTTTCCTCATCATCATGATCTGCAGCAGCATTACCAACAACAGCAGTCGTCGGTTGTTCCAATTGCGAAGCAGCAAAAGGTGAGTTCTATTACTACCGGAGATGATGCAAGCATCCAGCTTCAGCAGAGTATATTTGATCAGCTATTCAAAACTGCTGAGCTGATAAAAGCTGGTAATCCGGTTCAAGCGCAAGGGATATTGGCGCGGCTCAATCACCAGCTCTTACCAATTGGTAAGCCTTTTCAAAGGGCTTCTTTCTACATGAAAGAAGCCTTACAGTTAATGCTTCATTCAAACGGTAACAATCTCACGGCTTTTTCGCCGATTAGTTTCATTTTTAAAATTGGAGCTTATAAGTCTTTTTCCGAGATATCACCTGTTCTTCAGTTTGCCAACTTTACTTGTAATCAAGCCCTCATTGAAGCCTTGGAGAGGTTCGATCGAATTCATGTTATTGATTTTGATATTGGGTTTGGAGTACAATGGTCGTCTTTTATGCAAGAGATTGTACTAAGAAGTAACGGTAAGCCTTCACTTAAGATTACTGCTGTTGTATCTCCCTCGTCTTGCAACGAGATTGAGCTCAATTTCACCCAAGAAAATTTGAGTCAGTATGCAAAAGACCTCAACATGTCGTTTGAGTTCAACGTTTTGAACATTGAATCGTTGAACTTGCCATCATGTCCTCTACCGGGTCATTTCTTTGATAGTAATGAAGCCATTGGTGTGAACTTTCCTGTTTCCAGTTTCATAAGTAATCCATCATGCTTCCCTGTGGCTCTTCATTTTTTAAAGCAGCTAAGGCCGAAAGTGGTCGTAACTTTGGACAAAAACTGCGACCGAATGGACGTTCCTCTTCCCACCGATGTCATTCATGTTCTTCAATGTTACTCAGCTTTACTTGAATCACTTGATGCTGTGAACGTGAACCTCGATGTACTCCAAAAGATTGAAAGGCATTACATCCAACCAACCATCAACAAAATCATCCTCGGTCACCACAACCAGCGTGACAAATTACCTCCATGGAGGAACATGTTTCTTCAGTCCGGTTTCTCTCCCTTCACATTCAGCAAATTCACGGAAGCTCAAGCTGAGTGTTTGGTTCAAAGGGCACCTGTGAGAGGATTTCAAGTGGAAAGAAAGCCTTCCTCTCTTGTGCTATGCTGGCAGAGGAAAGAACTCATCTCAGTTTCAACTTGGAGATGTTGA
Protein sequence

>Msa0672200

MKALPLTFEEFQVKGMLDNFSSTSNSVSDSFSLLLNQPQQQNWLLDKEDYCYVEPTSVLDSRRSPSSPFSSSTLSSSHGSGSNGNGSNSNTSMVVFENLAHLSNDENSEEKCGGGGMRMEDWEGQDQSLLRLIMGDVEDPYAGLNKILQSGGCGSQNVDFHGGFGVLDHQQQQWLNMDASVQQGNYNVFPFIPENCNVLPLLDSGQEVFARRHQQQETQLPLFPHHHDLQQHYQQQQSSVVPIAKQQKVSSITTGDDASIQLQQSIFDQLFKTAELIKAGNPVQAQGILARLNHQLLPIGKPFQRASFYMKEALQLMLHSNGNNLTAFSPISFIFKIGAYKSFSEISPVLQFANFTCNQALIEALERFDRIHVIDFDIGFGVQWSSFMQEIVLRSNGKPSLKITAVVSPSSCNEIELNFTQENLSQYAKDLNMSFEFNVLNIESLNLPSCPLPGHFFDSNEAIGVNFPVSSFISNPSCFPVALHFLKQLRPKVVVTLDKNCDRMDVPLPTDVIHVLQCYSALLESLDAVNVNLDVLQKIERHYIQPTINKIILGHHNQRDKLPPWRNMFLQSGFSPFTFSKFTEAQAECLVQRAPVRGFQVERKPSSLVLCWQRKELISVSTWRC*