Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0673510 | XP_003605525.1 | 77.778 | 99 | 22 | 0 | 1 | 99 | 1 | 99 | 1.86e-47 | 161 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0673510 | sp|Q9FJK3|AGL80_ARATH | 56.000 | 100 | 44 | 0 | 1 | 100 | 1 | 100 | 5.28e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0673510 | G7JN85 | 77.778 | 99 | 22 | 0 | 1 | 99 | 1 | 99 | 8.88e-48 | 161 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0673510 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0673510 | MtrunA17_Chr4g0014321 | 77.778 | 99 | 22 | 0 | 1 | 99 | 1 | 99 | 1.71e-51 | 161 |
| Msa0673510 | MtrunA17_Chr4g0013041 | 71.717 | 99 | 26 | 1 | 1 | 99 | 1 | 97 | 1.21e-46 | 148 |
| Msa0673510 | MtrunA17_Chr8g0351751 | 62.376 | 101 | 36 | 1 | 1 | 99 | 1 | 101 | 2.38e-41 | 136 |
| Msa0673510 | MtrunA17_Chr2g0296831 | 62.626 | 99 | 37 | 0 | 1 | 99 | 1 | 99 | 1.64e-38 | 125 |
| Msa0673510 | MtrunA17_Chr3g0110131 | 60.606 | 99 | 39 | 0 | 1 | 99 | 1 | 99 | 6.13e-37 | 124 |
| Msa0673510 | MtrunA17_Chr4g0014031 | 62.626 | 99 | 37 | 0 | 1 | 99 | 1 | 99 | 8.55e-37 | 124 |
| Msa0673510 | MtrunA17_Chr4g0014121 | 65.657 | 99 | 34 | 0 | 1 | 99 | 29 | 127 | 1.54e-36 | 122 |
| Msa0673510 | MtrunA17_Chr3g0107381 | 50.505 | 99 | 49 | 0 | 1 | 99 | 1 | 99 | 2.86e-35 | 120 |
| Msa0673510 | MtrunA17_Chr3g0107331 | 48.958 | 96 | 49 | 0 | 4 | 99 | 3 | 98 | 7.76e-34 | 114 |
| Msa0673510 | MtrunA17_Chr4g0014131 | 60.606 | 99 | 39 | 0 | 1 | 99 | 1 | 99 | 9.38e-33 | 114 |
| Msa0673510 | MtrunA17_Chr5g0432691 | 46.465 | 99 | 53 | 0 | 1 | 99 | 1 | 99 | 3.49e-30 | 105 |
| Msa0673510 | MtrunA17_Chr3g0107341 | 45.263 | 95 | 52 | 0 | 5 | 99 | 4 | 98 | 6.17e-30 | 104 |
| Msa0673510 | MtrunA17_Chr2g0283941 | 45.918 | 98 | 53 | 0 | 1 | 98 | 1 | 98 | 1.50e-29 | 105 |
| Msa0673510 | MtrunA17_Chr3g0107351 | 44.211 | 95 | 53 | 0 | 5 | 99 | 4 | 98 | 2.89e-29 | 102 |
| Msa0673510 | MtrunA17_Chr3g0107361 | 43.158 | 95 | 54 | 0 | 5 | 99 | 4 | 98 | 3.48e-29 | 102 |
| Msa0673510 | MtrunA17_Chr3g0107461 | 42.268 | 97 | 56 | 0 | 1 | 97 | 1 | 97 | 2.18e-28 | 100 |
| Msa0673510 | MtrunA17_Chr5g0420351 | 42.424 | 99 | 56 | 1 | 1 | 99 | 1 | 98 | 1.46e-27 | 98.6 |
| Msa0673510 | MtrunA17_Chr1g0188681 | 43.299 | 97 | 54 | 1 | 1 | 97 | 1 | 96 | 7.01e-27 | 96.7 |
| Msa0673510 | MtrunA17_Chr1g0197461 | 42.708 | 96 | 54 | 1 | 4 | 99 | 3 | 97 | 5.04e-26 | 94.4 |
| Msa0673510 | MtrunA17_Chr1g0197321 | 40.404 | 99 | 58 | 1 | 1 | 99 | 1 | 98 | 6.57e-26 | 94.4 |
| Msa0673510 | MtrunA17_Chr1g0197261 | 42.424 | 99 | 56 | 1 | 1 | 99 | 1 | 98 | 2.23e-25 | 92.8 |
| Msa0673510 | MtrunA17_Chr7g0267601 | 42.105 | 95 | 55 | 0 | 4 | 98 | 7 | 101 | 5.99e-23 | 91.7 |
| Msa0673510 | MtrunA17_Chr4g0008701 | 40.000 | 95 | 57 | 0 | 4 | 98 | 7 | 101 | 3.65e-22 | 89.4 |
| Msa0673510 | MtrunA17_Chr2g0296861 | 33.684 | 95 | 63 | 0 | 1 | 95 | 1 | 95 | 6.95e-22 | 84.0 |
| Msa0673510 | MtrunA17_Chr1g0193311 | 43.299 | 97 | 54 | 1 | 1 | 97 | 1 | 96 | 1.77e-21 | 82.8 |
| Msa0673510 | MtrunA17_Chr4g0031421 | 47.826 | 69 | 36 | 0 | 29 | 97 | 1 | 69 | 2.45e-21 | 82.0 |
| Msa0673510 | MtrunA17_Chr7g0218151 | 39.394 | 99 | 59 | 1 | 1 | 99 | 1 | 98 | 8.46e-21 | 81.3 |
| Msa0673510 | MtrunA17_Chr3g0105791 | 46.377 | 69 | 37 | 0 | 29 | 97 | 1 | 69 | 2.16e-20 | 79.7 |
| Msa0673510 | MtrunA17_Chr1g0188661 | 38.384 | 99 | 60 | 1 | 1 | 99 | 1 | 98 | 1.51e-19 | 78.2 |
| Msa0673510 | MtrunA17_Chr3g0128721 | 37.778 | 90 | 56 | 0 | 4 | 93 | 7 | 96 | 6.64e-19 | 80.1 |
| Msa0673510 | MtrunA17_Chr1g0188651 | 42.268 | 97 | 55 | 1 | 1 | 97 | 1 | 96 | 7.26e-19 | 76.3 |
| Msa0673510 | MtrunA17_Chr4g0012761 | 40.206 | 97 | 57 | 1 | 1 | 97 | 1 | 96 | 1.21e-18 | 75.9 |
| Msa0673510 | MtrunA17_Chr3g0106501 | 40.845 | 71 | 42 | 0 | 29 | 99 | 1 | 71 | 5.49e-18 | 73.9 |
| Msa0673510 | MtrunA17_Chr3g0106421 | 42.254 | 71 | 41 | 0 | 29 | 99 | 1 | 71 | 9.04e-17 | 72.0 |
| Msa0673510 | MtrunA17_Chr1g0212151 | 36.000 | 100 | 57 | 3 | 5 | 98 | 5 | 103 | 8.14e-15 | 68.2 |
| Msa0673510 | MtrunA17_Chr6g0457851 | 37.000 | 100 | 56 | 4 | 5 | 98 | 15 | 113 | 6.34e-14 | 65.9 |
| Msa0673510 | MtrunA17_Chr6g0449861 | 37.000 | 100 | 56 | 3 | 5 | 98 | 13 | 111 | 2.73e-13 | 63.9 |
| Msa0673510 | MtrunA17_Chr6g0449851 | 36.000 | 100 | 57 | 3 | 5 | 98 | 5 | 103 | 3.35e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0673510 | AT5G48670.1 | 56.000 | 100 | 44 | 0 | 1 | 100 | 1 | 100 | 5.37e-30 | 108 |
| Msa0673510 | AT3G05860.2 | 43.000 | 100 | 57 | 0 | 1 | 100 | 1 | 100 | 8.48e-30 | 105 |
| Msa0673510 | AT3G05860.3 | 44.330 | 97 | 54 | 0 | 1 | 97 | 1 | 97 | 4.64e-29 | 105 |
| Msa0673510 | AT3G05860.1 | 44.330 | 97 | 54 | 0 | 1 | 97 | 1 | 97 | 6.57e-29 | 105 |
| Msa0673510 | AT5G26650.1 | 46.875 | 96 | 51 | 0 | 4 | 99 | 2 | 97 | 1.86e-27 | 103 |
| Msa0673510 | AT5G27960.1 | 46.875 | 96 | 51 | 0 | 4 | 99 | 2 | 97 | 4.63e-27 | 101 |
| Msa0673510 | AT1G65300.1 | 49.462 | 93 | 47 | 0 | 4 | 96 | 3 | 95 | 8.78e-27 | 99.8 |
| Msa0673510 | AT1G65330.1 | 46.739 | 92 | 49 | 0 | 5 | 96 | 4 | 95 | 3.04e-25 | 95.9 |
| Msa0673510 | AT5G27810.1 | 50.000 | 68 | 34 | 0 | 29 | 96 | 1 | 68 | 1.59e-21 | 82.4 |
| Msa0673510 | AT1G31630.1 | 40.206 | 97 | 55 | 2 | 5 | 101 | 4 | 97 | 5.81e-21 | 85.5 |
| Msa0673510 | AT5G26630.1 | 43.000 | 100 | 57 | 0 | 1 | 100 | 1 | 100 | 9.08e-21 | 82.8 |
| Msa0673510 | AT1G31640.1 | 38.947 | 95 | 57 | 1 | 5 | 99 | 4 | 97 | 1.21e-20 | 85.5 |
| Msa0673510 | AT2G28700.1 | 36.735 | 98 | 62 | 0 | 1 | 98 | 1 | 98 | 3.32e-20 | 83.2 |
| Msa0673510 | AT5G06500.1 | 39.216 | 102 | 62 | 0 | 1 | 102 | 1 | 102 | 9.93e-20 | 80.5 |
| Msa0673510 | AT1G22590.2 | 32.653 | 98 | 66 | 0 | 1 | 98 | 1 | 98 | 1.86e-19 | 78.2 |
| Msa0673510 | AT5G26580.1 | 39.394 | 99 | 46 | 1 | 1 | 99 | 1 | 85 | 3.71e-19 | 80.5 |
| Msa0673510 | AT2G40210.1 | 37.374 | 99 | 62 | 0 | 1 | 99 | 1 | 99 | 5.82e-18 | 77.4 |
| Msa0673510 | AT5G58890.1 | 38.462 | 91 | 46 | 3 | 1 | 81 | 1 | 91 | 1.20e-12 | 62.4 |
| Msa0673510 | AT5G55690.1 | 33.333 | 108 | 59 | 3 | 1 | 101 | 1 | 102 | 1.12e-11 | 59.7 |
| Msa0673510 | AT5G55690.2 | 33.333 | 108 | 59 | 3 | 1 | 101 | 1 | 102 | 1.12e-11 | 59.7 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCTTGTGCAGTAGTTTA+TGG | 0.155587 | 4_4:+19902762 | Msa0673510:CDS |
| CACAACAATGACTGGGAAAA+AGG | 0.190222 | 4_4:+19902632 | None:intergenic |
| TCATTGTCGATGATGATTCT+AGG | 0.260008 | 4_4:+19902667 | Msa0673510:CDS |
| AGATCTCTGGTTGAGGCTCA+TGG | 0.361064 | 4_4:-19902788 | None:intergenic |
| TGTGGTGATGGCCAGATCTC+TGG | 0.397343 | 4_4:-19902801 | None:intergenic |
| GGAAAGCAACATACAATAGA+AGG | 0.409123 | 4_4:+19902688 | Msa0673510:CDS |
| GATCTCTGGTTGAGGCTCAT+GGG | 0.428787 | 4_4:-19902787 | None:intergenic |
| TCAAAATTCAGAACAATGAC+TGG | 0.434871 | 4_4:+19902843 | Msa0673510:CDS |
| AAGAGACTTACATGAAAGAA+AGG | 0.463147 | 4_4:+19902895 | Msa0673510:CDS |
| ATGAGCCTCAACCAGAGATC+TGG | 0.467494 | 4_4:+19902790 | Msa0673510:CDS |
| ATCTCTGGTTGAGGCTCATG+GGG | 0.492174 | 4_4:-19902786 | None:intergenic |
| AGAGATCTGGCCATCACCAC+AGG | 0.494291 | 4_4:+19902803 | Msa0673510:CDS |
| ATCACCACAGGGAGTTCAAA+CGG | 0.524260 | 4_4:+19902815 | Msa0673510:CDS |
| AGAATCATCATCGACAATGA+AGG | 0.542377 | 4_4:-19902664 | None:intergenic |
| GATGGCCAGATCTCTGGTTG+AGG | 0.555241 | 4_4:-19902795 | None:intergenic |
| AACATACAATAGAAGGCGGA+AGG | 0.566279 | 4_4:+19902695 | Msa0673510:CDS |
| GTTTGAACTCCCTGTGGTGA+TGG | 0.567517 | 4_4:-19902813 | None:intergenic |
| AGAGACTTACATGAAAGAAA+GGG | 0.577279 | 4_4:+19902896 | Msa0673510:CDS |
| GCGGAAGGGTCTAATGATGA+AGG | 0.578779 | 4_4:+19902710 | Msa0673510:CDS |
| TATGAACTAAGCACTTTGTG+TGG | 0.579542 | 4_4:+19902735 | Msa0673510:CDS |
| CAAAATTCAGAACAATGACT+GGG | 0.592691 | 4_4:+19902844 | Msa0673510:CDS |
| GCGGAAGAAGAGCAACAAAA+CGG | 0.630975 | 4_4:+19902866 | Msa0673510:CDS |
| ACATACAATAGAAGGCGGAA+GGG | 0.677825 | 4_4:+19902696 | Msa0673510:CDS |
| GAGATCTGGCCATCACCACA+GGG | 0.678612 | 4_4:+19902804 | Msa0673510:CDS |
| AAGCAACATACAATAGAAGG+CGG | 0.694844 | 4_4:+19902691 | Msa0673510:CDS |
| AATTCAGAACAATGACTGGG+CGG | 0.724414 | 4_4:+19902847 | Msa0673510:CDS |
| AGCACCGTTTGAACTCCCTG+TGG | 0.787870 | 4_4:-19902819 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTAAAAACTGAAGATACAA+AGG | + | chr4_4:19902920-19902939 | Msa0673510:CDS | 25.0% |
| TCAAAATTCAGAACAATGAC+TGG | + | chr4_4:19902843-19902862 | Msa0673510:CDS | 30.0% | |
| CAAAATTCAGAACAATGACT+GGG | + | chr4_4:19902844-19902863 | Msa0673510:CDS | 30.0% | |
| AAGAGACTTACATGAAAGAA+AGG | + | chr4_4:19902895-19902914 | Msa0673510:CDS | 30.0% | |
| AGAGACTTACATGAAAGAAA+GGG | + | chr4_4:19902896-19902915 | Msa0673510:CDS | 30.0% | |
| AGAATCATCATCGACAATGA+AGG | - | chr4_4:19902667-19902686 | None:intergenic | 35.0% | |
| TCATTGTCGATGATGATTCT+AGG | + | chr4_4:19902667-19902686 | Msa0673510:CDS | 35.0% | |
| GGAAAGCAACATACAATAGA+AGG | + | chr4_4:19902688-19902707 | Msa0673510:CDS | 35.0% | |
| AAGCAACATACAATAGAAGG+CGG | + | chr4_4:19902691-19902710 | Msa0673510:CDS | 35.0% | |
| TATGAACTAAGCACTTTGTG+TGG | + | chr4_4:19902735-19902754 | Msa0673510:CDS | 35.0% | |
| AACATACAATAGAAGGCGGA+AGG | + | chr4_4:19902695-19902714 | Msa0673510:CDS | 40.0% | |
| ACATACAATAGAAGGCGGAA+GGG | + | chr4_4:19902696-19902715 | Msa0673510:CDS | 40.0% | |
| GAAGCTTGTGCAGTAGTTTA+TGG | + | chr4_4:19902762-19902781 | Msa0673510:CDS | 40.0% | |
| AATTCAGAACAATGACTGGG+CGG | + | chr4_4:19902847-19902866 | Msa0673510:CDS | 40.0% | |
| ATCACCACAGGGAGTTCAAA+CGG | + | chr4_4:19902815-19902834 | Msa0673510:CDS | 45.0% | |
| GCGGAAGAAGAGCAACAAAA+CGG | + | chr4_4:19902866-19902885 | Msa0673510:CDS | 45.0% | |
| GCGGAAGGGTCTAATGATGA+AGG | + | chr4_4:19902710-19902729 | Msa0673510:CDS | 50.0% | |
| ATCTCTGGTTGAGGCTCATG+GGG | - | chr4_4:19902789-19902808 | None:intergenic | 50.0% | |
| GATCTCTGGTTGAGGCTCAT+GGG | - | chr4_4:19902790-19902809 | None:intergenic | 50.0% | |
| AGATCTCTGGTTGAGGCTCA+TGG | - | chr4_4:19902791-19902810 | None:intergenic | 50.0% | |
| ATGAGCCTCAACCAGAGATC+TGG | + | chr4_4:19902790-19902809 | Msa0673510:CDS | 50.0% | |
| GTTTGAACTCCCTGTGGTGA+TGG | - | chr4_4:19902816-19902835 | None:intergenic | 50.0% | |
| GATGGCCAGATCTCTGGTTG+AGG | - | chr4_4:19902798-19902817 | None:intergenic | 55.0% | |
| !! | TGTGGTGATGGCCAGATCTC+TGG | - | chr4_4:19902804-19902823 | None:intergenic | 55.0% |
| AGAGATCTGGCCATCACCAC+AGG | + | chr4_4:19902803-19902822 | Msa0673510:CDS | 55.0% | |
| GAGATCTGGCCATCACCACA+GGG | + | chr4_4:19902804-19902823 | Msa0673510:CDS | 55.0% | |
| AGCACCGTTTGAACTCCCTG+TGG | - | chr4_4:19902822-19902841 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 19902639 | 19902947 | 19902639 | ID=Msa0673510;Name=Msa0673510 |
| chr4_4 | mRNA | 19902639 | 19902947 | 19902639 | ID=Msa0673510-mRNA-1;Parent=Msa0673510;Name=Msa0673510-mRNA-1;_AED=0.43;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|102 |
| chr4_4 | exon | 19902639 | 19902947 | 19902639 | ID=Msa0673510-mRNA-1:exon:5755;Parent=Msa0673510-mRNA-1 |
| chr4_4 | CDS | 19902639 | 19902947 | 19902639 | ID=Msa0673510-mRNA-1:cds;Parent=Msa0673510-mRNA-1 |
| Gene Sequence |
| Protein sequence |
